291,072 results match your criteria Bioengineering & translational medicine[Journal]


Risk of Bleeding with Exposure to Warfarin and Nonsteroidal Anti-Inflammatory Drugs: A Systematic Review and Meta-Analysis.

Thromb Haemost 2020 May 26. Epub 2020 May 26.

College of Pharmacy, L. S. Skaggs Research Institute, University of Utah, Salt Lake City, Utah, United States.

Background:  Warfarin use can trigger the occurrence of bleeding independently or as a result of a drug-drug interaction when used in combination with nonsteroidal anti-inflammatory drugs (NSAIDs).

Objectives:  This article examines the risk of bleeding in individuals exposed to concomitant warfarin and NSAID compared with those taking warfarin alone (Prospero Registry ID 145237).

Methods:  PubMed, EMBASE, Scopus, and Web of Science were searched. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1055/s-0040-1710592DOI Listing

Pro-inflammatory Cytokine Interleukin 1β Disrupts β cell Circadian Clock Function and Regulation of Insulin Secretion.

Endocrinology 2020 May 26. Epub 2020 May 26.

Department of Physiology and Biomedical Engineering, Mayo Clinic College of Medicine and Science, Rochester, Minnesota.

Intrinsic β cell circadian clocks are important regulators of insulin secretion and overall glucose homeostasis. Whether the circadian clock in β cells is perturbed following exposure to pro-diabetogenic stressors such as pro-inflammatory cytokines, and whether these perturbations are featured during the development of diabetes, remains unknown. To address this, we examined the effects of cytokine-mediated inflammation common to the pathophysiology of diabetes, on the physiological and molecular regulation of the β cell circadian clock. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1210/endocr/bqaa084DOI Listing

Indocyanine Green-Coated Polycaprolactone Micelles for Fluorescence Imaging of Tumors.

ACS Appl Bio Mater 2020 Apr 8;3(4):2344-2349. Epub 2020 Mar 8.

Department of Bioengineering, School of Engineering and Applied Sciences, University of Pennsylvania, Philadelphia, Pennsylvania 19104, United States.

Recently, near-infrared (NIR) fluorescent dyes such as indocyanine green (ICG) have received tremendous interest as contrast agents for use in fluorescence-guided, intraoperative cancer resection surgery. However, despite showing great promise, ICG has many shortcomings such as rapid clearance and poor tumor accumulation. To improve the selective accumulation of ICG within tumors, numerous groups have formulated ICG into nanoparticles, but these approaches can suffer from rapid leakage of ICG, use of materials that exhibit poor or incomplete excretion, or complex chemistries that are not easily amenable to scale up for clinical use. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsabm.0c00091DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7245056PMC

Cell shape, and not 2D migration, predicts extracellular matrix-driven 3D cell invasion in breast cancer.

APL Bioeng 2020 Jun 5;4(2):026105. Epub 2020 May 5.

Department of Biomedical Engineering, Tufts University, Medford, Massachusetts 02155, USA.

Metastasis, the leading cause of death in cancer patients, requires the invasion of tumor cells through the stroma in response to migratory cues, in part provided by the extracellular matrix (ECM). Recent advances in proteomics have led to the identification of hundreds of ECM proteins, which are more abundant in tumors relative to healthy tissue. Our goal was to develop a pipeline to easily predict which ECM proteins are more likely to have an effect on cancer invasion and metastasis. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1063/1.5143779DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7202897PMC

Dual-Surfactant-Capped Ag Nanoparticles as a Highly Selective and Sensitive Colorimetric Sensor for Citrate Detection.

ACS Omega 2020 May 7;5(19):10696-10703. Epub 2020 May 7.

School of Chemical Engineering, Sungkyunkwan University, Suwon 16419, Republic of Korea.

A colorimetric sensor for the detection of citrate ions is reported here using dual-surfactant-capped Ag nanoparticles (dual-AgNP sensor). A mixture of cetyl trimethyl ammonium bromide and a newly prepared gemini nonionic (GFEO) surfactant was used as a capping agent to synthesize dual-surfactant-capped Ag NPs for selective and sensitive citrate detection. The GFEO surfactant was designed with a specific chemical structure to provide strong binding with citrate for selective and sensitive detection. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1021/acsomega.9b04199DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7240824PMC

Using transcranial magnetic stimulation to map the cortical representation of lower-limb muscles.

Clin Neurophysiol Pract 2020 29;5:87-99. Epub 2020 Apr 29.

School of Healthcare Sciences, Cardiff University, United Kingdom.

Objective: To evaluate the extent to which transcranial magnetic stimulation (TMS) can identify discrete cortical representation of lower-limb muscles in healthy individuals.

Methods: Motor evoked potentials were recorded from resting vastus medialis, rectus femoris, vastus lateralis, medial and lateral hamstring, and medial and lateral gastrocnemius muscles on the right leg of 16 young healthy adults using bipolar surface electrodes. TMS was delivered through a 110-mm double-cone coil at 63 sites over the left hemisphere. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.cnp.2020.04.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7235616PMC

Lessons learned from conventional animals: Encouragement to use specific-pathogen-free animals.

Regen Ther 2020 Jun 16;14:296-298. Epub 2020 May 16.

Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, Tokyo, Japan.

Experimental rabbits provide evidence for translational research regarding the pathogenies or treatment of human diseases. We developed a novel method for regenerating the middle ear mucosa using autologous cultured nasal mucosal epithelial cell sheets, and evaluated the wound healing process in the middle ear mucosa of experimental rabbits. Nonetheless, vigilant microbiological monitoring of experimental animals is essential to effectively prevent a decline in their health conditions, which may affect the research results. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.reth.2020.03.003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7235937PMC

Intelligent Diagnosis of Heart Murmurs in Children with Congenital Heart Disease.

J Healthc Eng 2020 9;2020:9640821. Epub 2020 May 9.

Department of Mechanical Engineering, Texas Tech University, Lubbock, TX 79409, USA.

Heart auscultation is a convenient tool for early diagnosis of heart diseases and is being developed to be an intelligent tool used in online medicine. Currently, there are few studies on intelligent diagnosis of pediatric murmurs due to congenital heart disease (CHD). The purpose of the study was to develop a method of intelligent diagnosis of pediatric CHD murmurs. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1155/2020/9640821DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7238385PMC

Closed-loop feedback control of microbubble diameter from a flow-focusing microfluidic device.

Biomicrofluidics 2020 May 7;14(3):034101. Epub 2020 May 7.

Department of Biomedical Engineering, University of Virginia, Charlottesville 22908, USA.

Real-time observation and control of particle size and production rate in microfluidic devices are important capabilities for a number of applications, including the production, sorting, and manipulation of microbubbles and droplets. The production of microbubbles from flow-focusing microfluidic devices had been investigated in multiple studies, but each lacked an approach for on-chip measurement and control of microbubble diameter in real time. In this work, we implement a closed-loop feedback control system in a flow-focusing microfluidic device with integrated on-chip electrodes. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1063/5.0005205DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7211089PMC

Synthesis and Anticancer and Antimicrobial Evaluation of Novel Ether-linked Derivatives of Ornidazole.

Turk J Pharm Sci 2020 Feb 19;17(1):81-93. Epub 2020 Feb 19.

Marmara University, Faculty of Pharmacy, Department of Pharmaceutical Chemistry, Haydarpaşa, İstanbul, Turkey.

Objectives: Some novel 1-(2-methyl-5-nitro-1H-imidazol-1-yl)-3-(substituted phenoxy)propan-2-ol derivatives were designed and synthesized.

Materials And Methods: Compounds were obtained by refluxing ornidazole with the corresponding phenolic compounds in the presence of anhydrous KCO in acetonitrile.

Results: Following the structure elucidation, the antimicrobial activity and cytotoxic effects of compounds on K562 leukemia and NIH/3T3 mouse embryonic fibroblast cells were measured. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.4274/tjps.galenos.2018.59389DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7227874PMC
February 2020

Identifying the Common and Subject-specific Functional Units of Speech Movements via a Joint Sparse Non-negative Matrix Factorization Framework.

Proc SPIE Int Soc Opt Eng 2020 Feb 10;11313. Epub 2020 Mar 10.

Gordon Center for Medical Imaging, Department of Radiology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA.

The tongue is capable of producing intelligible speech because of successful orchestration of muscle groupings-i.e., functional units-of the highly complex muscles over time. Read More

View Article

Download full-text PDF

Source
February 2020

Neonatal EEG sleep stage classification based on deep learning and HMM.

J Neural Eng 2020 May 26. Epub 2020 May 26.

faculty of Medicine, University of Picardie-Jules Verne, Amiens, FRANCE.

Automatic sleep stage scoring is of great importance for investigating sleep architecture during infancy. In this work, we introduce a novel multichannel approach based on deep learning networks and hidden Markov models (HMM) to improve the accuracy of sleep stage classification in term neonates. The classification performance was evaluated on quiet sleep (QS) and active sleep (AS) stages, each with two sub-states, using multichannel EEG data recorded from sixteen neonates with postmenstrual age of 38-40 weeks. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1088/1741-2552/ab965aDOI Listing

Modeling the effects of intervention strategies on COVID-19 transmission dynamics.

J Clin Virol 2020 May 15;128:104440. Epub 2020 May 15.

Anhembi Morumbi University, Biomedical Engineering Department, São Paulo, SP, Brazil; Arena235 Research Lab - São José dos Campos, SP, Brazil; Center for Innovation, Technology and Education - CITE, Parque Tecnológico de São José dos Campos, São José dos Campos, SP, Brazil. Electronic address:

Objectives: To model the effects of continuous, intermittent, and stepping-down social distancing (SD) strategies and personal protection measures on COVID-19 transmission dynamics.

Methods: Constant, intermittent, and stepping-down SD strategies were modeled at 4 mean magnitudes (5%, 10 %, 15 % and 20 %), 2 time windows (40-days, 80-days), and 2 levels of personal caution (30 % and 50 %).

Results: The stepping-down strategy was the best long-term SD strategy to minimize the peak number of active COVID-19 cases and associated deaths. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jcv.2020.104440DOI Listing

Accurate and efficient sequential ensemble learning for highly imbalanced multi-class data.

Authors:
Chi-Man Vong Jie Du

Neural Netw 2020 May 19;128:268-278. Epub 2020 May 19.

School of Biomedical Engineering, Health Science Center, Shenzhen University, Shenzhen, 518060, China. Electronic address:

Multi-class classification for highly imbalanced data is a challenging task in which multiple issues must be resolved simultaneously, including (i) accuracy on classifying highly imbalanced multi-class data; (ii) training efficiency for large data; and (iii) sensitivity to high imbalance ratio (IR). In this paper, a novel sequential ensemble learning (SEL) framework is designed to simultaneously resolve these issues. SEL framework provides a significant property over traditional AdaBoost, in which the majority samples can be divided into multiple small and disjoint subsets for training multiple weak learners without compromising accuracy (while AdaBoost cannot). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.neunet.2020.05.010DOI Listing

Effect of variations in tissue-level ductility on human vertebral strength.

Bone 2020 May 23:115445. Epub 2020 May 23.

Department of Mechanical Engineering, University of California, Berkeley, CA, USA; Department of Bioengineering, University of California, Berkeley, CA, USA. Electronic address:

Although the ductility of bone tissue is a unique element of bone quality and varies with age and across the population, the extent to which and mechanisms by which typical population-variations in tissue-level ductility can alter whole-bone strength remains unclear. To provide insight, we conducted a finite element analysis parameter study of whole-vertebral (monotonic) compressive strength on six human L1 vertebrae. Each model was generated from micro-CT scans, capturing the trabecular micro-architecture in detail, and included a non-linear constitutive model for the bone tissue that allowed for plastic yielding, different strengths in tension and compression, large deformations, and, uniquely, localized damage once a specified limit in tissue-level ultimate strain was exceeded. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bone.2020.115445DOI Listing

iTRAQ-based comparative proteomic analysis reveals high temperature accelerated leaf senescence of tobacco (Nicotiana tabacum L.) during flue-curing.

Genomics 2020 May 23. Epub 2020 May 23.

Department of Agronomy, College of Agriculture and Biotechnology, Key Laboratory of Crop Germplasm Resource, Zhejiang University, Hangzhou 310058, PR China. Electronic address:

Tobacco (Nicotiana tabacum) is extensively cultivated all over the world for its economic value. During curing and storage, senescence occurs, which is associated with physiological and biochemical changes in postharvest plant organs. However, the molecular mechanisms involved in accelerated senescence due to high temperatures in tobacco leaves during curing need further elaboration. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ygeno.2020.05.019DOI Listing

Copper mediates mitochondrial biogenesis in retinal pigment epithelial cells.

Biochim Biophys Acta Mol Basis Dis 2020 May 23:165843. Epub 2020 May 23.

R S Mehta Jain Department of Biochemistry and Cell Biology, KBIRVO, Vision Research Foundation, Sankara Nethralaya, College Road, Nungambakkam, Chennai - 6, India. Electronic address:

Age related macular degeneration (AMD) is a multifactorial disease with genetic, biochemical and environmental risk factors. We observed a significant increase in copper levels in choroid-RPE from donor eyeballs with AMD. Adult retinal pigment epithelial cells (ARPE19 cells) exposed to copper in-vitro showed a 2-fold increase in copper influx transporter CTR1 and copper uptake at 50 μM concentration. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbadis.2020.165843DOI Listing

Morphological, chemical and thermal analysis of cellulose nanocrystals extracted from bamboo fibre.

Int J Biol Macromol 2020 May 23. Epub 2020 May 23.

Center of Excellence for Advanced Materials Research, King Abdulaziz University, P.O. Box 80203, Jeddah 21589, Saudi Arabia; Chemistry Department, Faculty of Science, King Abdulaziz University, P.O. Box 80203, Jeddah 21589, Saudi Arabia.

This work investigates the extraction of cellulose nanocrystals (CNC) from bamboo fibre as an alternative approach to utilize the waste bamboo fibre. In this study, bamboo fibre was subjected to acid hydrolysis for efficient isolation of CNC from bamboo fibre. The extracted CNC's were morphologically, characterized via Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM) and Atomic Force Microscopy (AFM). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ijbiomac.2020.05.170DOI Listing

Phosphorylation of the acyl-CoA binding pocket of the FadR transcription regulator in Sulfolobus acidocaldarius.

Biochimie 2020 May 23. Epub 2020 May 23.

Research Group of Microbiology, Department of Bioengineering Sciences, Vrije Universiteit Brussel, Pleinlaan 2, B-1050, Brussels, Belgium. Electronic address:

The archaeal model organism Sulfolobus acidocaldarius possesses a TetR-like transcription factor that represses a 30-kb gene cluster encoding fatty acid metabolism enzymes. Interaction of this regulator, FadR, with acyl-CoA molecules causes a DNA dissociation, which may lead to a derepression of the gene cluster. Previously, a phosphoproteome analysis revealed the phosphorylation of three consecutive amino acids in the acyl-CoA ligand binding pocket. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biochi.2020.05.007DOI Listing

Lentivirally administered glial cell line-derived neurotrophic factor promotes post-ischemic neurological recovery, brain remodeling and contralesional pyramidal tract plasticity by regulating axonal growth inhibitors and guidance proteins.

Exp Neurol 2020 May 23:113364. Epub 2020 May 23.

Regenerative and Restorative Medicine Research Center (REMER), Istanbul Medipol University, Istanbul, Turkey; Dept. of Physiology, Faculty of Medicine, Istanbul Medipol University, Istanbul, Turkey. Electronic address:

Owing to its potent longterm neuroprotective and neurorestorative properties, glial cell line-derived neurotrophic factor (GDNF) is currently studied in neurodegenerative disease clinical trials. However, little is known about the longterm effect of GDNF on neurological recovery, brain remodeling and neuroplasticity in the post-acute phase of ischemic stroke. In a comprehensive set of experiments, we examined the effects of lentiviral GDNF administration after ischemic stroke. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.expneurol.2020.113364DOI Listing

Paradoxical aortic stiffening and subsequent cardiac dysfunction in Hutchinson-Gilford progeria syndrome.

J R Soc Interface 2020 May 27;17(166):20200066. Epub 2020 May 27.

Department of Biomedical Engineering, Yale University, New Haven, CT, USA.

Hutchinson-Gilford progeria syndrome (HGPS) is an ultra-rare disorder with devastating sequelae resulting in early death, presently thought to stem primarily from cardiovascular events. We analyse novel longitudinal cardiovascular data from a mouse model of HGPS () using allometric scaling, biomechanical phenotyping, and advanced computational modelling and show that late-stage diastolic dysfunction, with preserved systolic function, emerges with an increase in the pulse wave velocity and an associated loss of aortic function, independent of sex. Specifically, there is a dramatic late-stage loss of smooth muscle function and cells and an excessive accumulation of proteoglycans along the aorta, which result in a loss of biomechanical function (contractility and elastic energy storage) and a marked structural stiffening despite a distinctly low intrinsic material stiffness that is consistent with the lack of functional lamin A. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1098/rsif.2020.0066DOI Listing

Programming and simulating chemical reaction networks on a surface.

J R Soc Interface 2020 May 27;17(166):20190790. Epub 2020 May 27.

Bioengineering, California Institute of Technology, Pasadena, CA 91125, USA.

Models of well-mixed chemical reaction networks (CRN) have provided a solid foundation for the study of programmable molecular systems, but the importance of spatial organization in such systems has increasingly been recognized. In this paper, we explore an alternative chemical computing model introduced by Qian & Winfree in 2014, the surface CRN, which uses molecules attached to a surface such that each molecule only interacts with its immediate neighbours. Expanding on the constructions in that work, we first demonstrate that surface CRNs can emulate asynchronous and synchronous deterministic cellular automata and implement continuously active Boolean logic circuits. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1098/rsif.2019.0790DOI Listing

Ultrasensitive detection of microRNA using a bismuthene-enabled fluorescence quenching biosensor.

Chem Commun (Camb) 2020 May 26. Epub 2020 May 26.

State Key Laboratory of Automotive Simulation and Control, School of Materials Science and Engineering, Key Laboratory of Automobile Materials of MOE, Jilin University, Changchun 130012, P. R. China.

Bismuthene, a monoelemental two-dimensional material, has shown promise in the biomedical, electronic, and energy fields due to its high carrier mobility and stability at room temperature. However, its use in biosensing applications is restricted due to its undefined quenching mechanism for dye molecules. Herein, we developed a novel ultrathin bismuthene-based sensing platform for microRNA (miRNA)-specific detection that even discriminates single-base mismatches. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/d0cc01004aDOI Listing

Effectiveness of electrical stimulation on nerve regeneration after crush injury: Comparison between invasive and non-invasive stimulation.

PLoS One 2020 26;15(5):e0233531. Epub 2020 May 26.

Department of Creative IT Engineering, Pohang University of Science and Technology, Pohang, Republic of Korea.

Several studies have investigated the use of invasive and non-invasive stimulation methods to enhance nerve regeneration, and varying degrees of effectiveness have been reported. However, due to the use of different parameters in these studies, a fair comparison between the effectiveness of invasive and non-invasive stimulation methods is not possible. The present study compared the effectiveness of invasive and non-invasive stimulation using similar parameters. Read More

View Article

Download full-text PDF

Source
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0233531PLOS

Myocardial protection by nanomaterials formulated with CHIR99021 and FGF1.

JCI Insight 2020 May 26. Epub 2020 May 26.

Department of Biomedical Engineering, University of Alabama at Birmingham, Birmingham, United States of America.

The mortality of patients suffering from acute myocardial infarction (AMI) is linearly related to the infarct size. As regeneration of cardiomyocytes from cardiac progenitor cells is minimal in the mammalian adult heart, we have explored a new therapeutic approach which leverages the capacity of nanomaterials to release chemicals over time to promote myocardial protection and infarct size reduction. Initial screening identified two chemicals, FGF1 and CHIR99021 (a Wnt1 agonist/GSK-3ß antagonist) which synergistically enhance cardiomyocyte cell cycle in vitro. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1172/jci.insight.132796DOI Listing

Current Approaches in Treatment of Diabetic Retinopathy and Future Perspectives.

J Ocul Pharmacol Ther 2020 May 21. Epub 2020 May 21.

Depatment of Bioengineering, Yıldız Technical University, Istanbul, Turkey.

Diabetes mellitus (DM) is a metabolic disease, which is the most common cause of low vision in developing countries and affects almost all systems of the body. In view of the increase in DM prevalence in the world, it would not be a surprise that diabetic retinopathy (DR) and other vascular complications related to diabetes become a serious public health problem. Currently, vascular endothelial growth factor, laser photocoagulation, and intravitreal steroids are the mainstays for DR treatment, but the efficacy of these treatment strategies remains insufficient. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1089/jop.2019.0137DOI Listing

Molecular Imaging of Infective Endocarditis With 6''-[F]Fluoromaltotriose Positron Emission Tomography-Computed Tomography.

Circulation 2020 May 26;141(21):1729-1731. Epub 2020 May 26.

Department of Radiology, Molecular Imaging Program at Stanford (MIPS), Bio-X Program (M.W., G.G., E.C., M.N., T.H., M.T.G., J.C.W., S.S.G.), Stanford University School of Medicine, CA.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1161/CIRCULATIONAHA.119.043924DOI Listing

Single-Cell Transcriptomics: New Insights in Heart Research.

Circulation 2020 May 26;141(21):1720-1723. Epub 2020 May 26.

Department of Biomedical Engineering, School of Medicine, School of Engineering, University of Alabama at Birmingham, Birmingham, AL, USA.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1161/CIRCULATIONAHA.120.046043DOI Listing

Excipient Enhanced Growth Aerosol Surfactant Replacement Therapy in an Rat Lung Injury Model.

J Aerosol Med Pulm Drug Deliv 2020 May 21. Epub 2020 May 21.

Department of Biomedical Engineering, Virginia Commonwealth University, Richmond, Virginia, USA.

In neonatal respiratory distress syndrome, breathing support and surfactant therapy are commonly used to enable the alveoli to expand. Surfactants are typically delivered through liquid instillation. However, liquid instillation does not specifically target the small airways. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1089/jamp.2020.1593DOI Listing

Deep-Learning-Based Characterization of Tumor-Infiltrating Lymphocytes in Breast Cancers From Histopathology Images and Multiomics Data.

JCO Clin Cancer Inform 2020 May;4:480-490

Department of Medicine, Indiana University School of Medicine, Indianapolis, IN.

Purpose: Tumor-infiltrating lymphocytes (TILs) and their spatial characterizations on whole-slide images (WSIs) of histopathology sections have become crucial in diagnosis, prognosis, and treatment response prediction for different cancers. However, fully automatic assessment of TILs on WSIs currently remains a great challenge because of the heterogeneity and large size of WSIs. We present an automatic pipeline based on a cascade-training U-net to generate high-resolution TIL maps on WSIs. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1200/CCI.19.00126DOI Listing

A Three-way Accuracy Comparison of the Dexcom G5, Abbott Freestyle Libre Pro, and Senseonics Eversense CGM Devices in an Home-Use Study of Subjects with Type 1 Diabetes.

Diabetes Technol Ther 2020 May 26. Epub 2020 May 26.

Massachusetts General Hospital, 2348, Endocrinology, Boston, Massachusetts, United States;

Background: There is a dearth of comparative accuracy studies of continuous glucose monitoring (CGM) devices in the home-use setting, and none with the Eversense implantable CGM.

Methods: We evaluated the accuracy of the Dexcom G5, Abbott Freestyle Libre Pro, and Senseonics Eversense during a 6-week, free-living, home-use bionic pancreas study involving 23 subjects with type 1 diabetes who wore all 3 devices concurrently. The primary outcome was the mean absolute relative difference (MARD) between CGM readings and point-of-care (POC) plasma-glucose (PG) values obtained approximately twice daily by the subjects. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1089/dia.2019.0449DOI Listing

In Situ Ni Stain for Liposome Imaging by Liquid-Cell Transmission Electron Microscopy.

Nano Lett 2020 May 26. Epub 2020 May 26.

Department of Chemistry, Northwestern University, Evanston, Illinois 60208, United States.

Solvated soft matter, both biological and synthetic, can now be imaged in liquids using liquid-cell transmission electron microscopy (LCTEM). However, such systems are usually composed solely of organic molecules (low elements) producing low contrast in TEM, especially within thick liquid films. We aimed to visualize liposomes by LCTEM rather than requiring cryogenic TEM (cryoTEM). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1021/acs.nanolett.0c00898DOI Listing

Artificial Biosystem for Modulation of Interactions between Antigen-Presenting Cells and T Cells.

Adv Biosyst 2020 May 26:e2000039. Epub 2020 May 26.

Laboratory for Immuno Bioengineering Research and Applications, Division of Engineering, New York University Abu Dhabi, Abu Dhabi, UAE.

T cell activation is triggered by signal molecules on the surface of antigen-presenting cells (APC) and subsequent exertion of cellular forces. Deciphering the biomechanical and biochemical signals in this complex process is of interest and will contribute to an improvement in immunotherapy strategies. To address underlying questions, coculture and biomimetic models are established. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/adbi.202000039DOI Listing

Machine Learning and Multiparametric Brain MRI to Differentiate Hereditary Diffuse Leukodystrophy with Spheroids from Multiple Sclerosis.

J Neuroimaging 2020 May 26. Epub 2020 May 26.

Department of Clinical Neuroscience, Karolinska Institutet, Stockholm, Sweden.

Background And Purpose: Hereditary diffuse leukoencephalopathy with spheroids (HDLS) and multiple sclerosis (MS) are demyelinating and neurodegenerative disorders that can be hard to distinguish clinically and radiologically. HDLS is a rare disorder compared to MS, which has led to occurrent misdiagnosis of HDLS as MS. That is problematic since their prognosis and treatment differ. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/jon.12725DOI Listing

Association of Intrauterine Growth Restriction and Small for Gestational Age Status With Childhood Cognitive Outcomes: A Systematic Review and Meta-analysis.

JAMA Pediatr 2020 May 26. Epub 2020 May 26.

Department of Developmental Psychology and Socialization, University of Padova, Padova, Italy.

Importance: The magnitude of the association of intrauterine growth restriction (IUGR) and small for gestational age (SGA) status with cognitive outcomes in preterm and term-born children has not been established.

Objective: To examine cognitive outcomes of preterm and term-born children who had IUGR and were SGA compared with children who were appropriate for gestational age (AGA) during the first 12 years of life.

Data Sources: For this systematic review and meta-analysis, the Scopus, PubMed, Web of Science, Science Direct, PsycInfo, and ERIC databases were searched for English-language, peer-reviewed literature published between January 1, 2000, and February 20, 2020. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1001/jamapediatrics.2020.1097DOI Listing

Four-dimensional Flow Magnetic Resonance Imaging Quantification of Blood Flow in Bicuspid Aortic Valve.

J Thorac Imaging 2020 May 22. Epub 2020 May 22.

Department of Radiology, Northwestern University, Chicago.

Background: Four-dimensional (D) flow magnetic resonance imaging (MRI) is limited by time-consuming and nonstandardized data analysis. We aimed to test the efficiency and interobserver reproducibility of a dedicated 4D flow MRI analysis workflow.

Materials And Methods: Thirty retrospectively identified patients with bicuspid aortic valve (BAV, age=47. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1097/RTI.0000000000000535DOI Listing

The Intelligent Automated Pressure-Adjustable Orthosis for Patients with Adolescent Idiopathic Scoliosis (AIS): A Bi-Center Randomized Controlled Trial.

Spine (Phila Pa 1976) 2020 May 21. Epub 2020 May 21.

Department of Biomedical Engineering, The Hong Kong Polytechnic University, Hong Kong, PRC.

Study Design: Randomized controlled trial.

Objective: To compare the effectiveness of the automated pressure-adjustable orthosis (PO) and conventional orthosis (CO) for treatment of adolescent idiopathic scoliosis (AIS).

Summary Of Background Data: Orthosis wearing quality may influence its effectiveness for AIS. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1097/BRS.0000000000003559DOI Listing

Zero Echo Time-Based PET/MRI Attenuation Correction in Patients With Oral Cavity Cancer: Initial Experience.

Clin Nucl Med 2020 May 20. Epub 2020 May 20.

From the Biomedical Imaging Research Center.

Purpose: The aims of this study were to demonstrate the feasibility of zero echo time (ZTE) MRI for jawbone identification, and to evaluate the quantitative performance of F-FDG PET/MRI with ZTE-based attenuation correction (ZTE-AC) compared with PET/CT and PET/MRI with Dixon MR-based AC (Dixon-AC) in patients with oral cavity cancer (OCC).

Materials And Methods: Thirteen OCC patients underwent whole-body F-FDG PET/CT and subsequent regional PET/MRI with Dixon-AC and ZTE-AC in 1 day. SUVs of the primary OCC and metastatic cervical lymph nodes (CLNs) were measured on PET/CT (SUVCT), PET/MRI with Dixon-AC (SUVDixon), and ZTE-AC (SUVZTE). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1097/RLU.0000000000003091DOI Listing

Osteoclast and osteoblast response to strontium-doped struvite coatings on titanium for improved bone integration.

Biomed Tech (Berl) 2020 May 26. Epub 2020 May 26.

Department for Functional Materials in Medicine and Dentistry, University of Würzburg, Pleicherwall 2, 97070, Würzburg, Germany.

To develop implants with improved bone ingrowth, titanium substrates were coated with homogeneous and dense struvite (MgNH4PO4·6H2O) layers by means of electrochemically assisted deposition. Strontium nitrate was added to the coating electrolyte in various concentrations, in order to fabricate Sr-doped struvite coatings with Sr loading ranging from 10.6 to 115 μg/cm2. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1515/bmt-2019-0265DOI Listing

Signaling diversity enabled by Rap1-regulated plasma membrane ERK with distinct temporal dynamics.

Elife 2020 May 26;9. Epub 2020 May 26.

Department of Pharmacology, Department of Bioengineering, Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, United States.

A variety of different signals induce specific responses through a common, Extracellular-signal regulated kinase (ERK)-dependent cascade. It has been suggested that signaling specificity can be achieved through precise temporal regulation of ERK activity. Given the wide distrubtion of ERK susbtrates across different subcellular compartments, it is important to understand how ERK activity is temporally regulated at specific subcellular locations. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.7554/eLife.57410DOI Listing

Invasion and Defense Interactions between Enzyme-Active Liquid Coacervate Protocells and Living Cells.

Small 2020 May 26:e2002073. Epub 2020 May 26.

State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University, Changsha, 410082, P. R. China.

The design and construction of mutual interaction models between artificial microsystems and living cells have the potential to open a wide range of novel applications in biomedical and biomimetic technologies. In this study, an artificial form of invasion-defense mutual interactions is established in a community of glucose oxidase (GOx)-containing liquid coacervate microdroplets and living cells, which interact via enzyme-mediated reactive oxygen species (ROS) damage. The enzyme-containing coacervate microdroplets, formed via liquid-liquid phase separation, act as invader protocells to electrostatically bind with the host HepG2 cell, resulting in assimilation. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/smll.202002073DOI Listing

Overcoming Obstacles in Pathological Diagnosis of Pulmonary Nodules through Circulating Tumor Cell Enrichment.

Small 2020 May 26:e2001695. Epub 2020 May 26.

Department of Respiratory Medicine, Shanghai Pulmonary Hospital, Tongji University, 507 Zhengmin Road, Shanghai, 200433, China.

With the popularity of low-dose computed tomography (LDCT) in clinical examination of the lung, the prevalence of pulmonary nodules has significantly increased, thus significantly improving the early diagnosis of lung cancer, but also potentially contributing to overtreatment. This study aims to develop a noninvasive method to assist in diagnosing the pulmonary nodules. To do so, 3798 patients are recruited from the Department of Thoracic Surgery at Shanghai Pulmonary Hospital and peripheral blood samples are collected from them before surgery. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/smll.202001695DOI Listing

Temporal Sampling of Enzymes from Live Cells by Localized Electroporation and Quantification of Activity by SAMDI Mass Spectrometry.

Small 2020 May 26:e2000584. Epub 2020 May 26.

Department of Mechanical Engineering, Northwestern University, Evanston, IL, 60208, USA.

Measuring changes in enzymatic activity over time from small numbers of cells remains a significant technical challenge. In this work, a method for sampling the cytoplasm of cells is introduced to extract enzymes and measure their activity at multiple time points. A microfluidic device, termed the live cell analysis device (LCAD), is designed, where cells are cultured in microwell arrays fabricated on polymer membranes containing nanochannels. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/smll.202000584DOI Listing

MicroRNA-204-5p modulates mitochondrial biogenesis in C2C12 myotubes and associates with oxidative capacity in humans.

J Cell Physiol 2020 May 26. Epub 2020 May 26.

Department of Nutrition and Movement Sciences, NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University, Maastricht, The Netherlands.

Using an unbiased high-throughput microRNA (miRNA)-silencing screen combined with functional readouts for mitochondrial oxidative capacity in C2C12 myocytes, we previously identified 19 miRNAs as putative regulators of skeletal muscle mitochondrial metabolism. In the current study, we highlight miRNA-204-5p, identified from this screen, and further studied its role in the regulation of skeletal muscle mitochondrial function. Following silencing of miRNA-204-5p in C2C12 myotubes, gene and protein expression were assessed using quantitative polymerase chain reaction, microarray analysis, and western blot analysis, while morphological changes were studied by confocal microscopy. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/jcp.29797DOI Listing

Design of nanomaterials for applications in maternal/fetal medicine.

J Mater Chem B 2020 May 26. Epub 2020 May 26.

Department of Biomedical Engineering, University of Delaware, Newark, DE 19716, USA. and Department of Materials Science and Engineering, University of Delaware, Newark, DE 19716, USA and Helen F. Graham Cancer Center & Research Institute, Newark, DE 19713, USA.

Pregnancy complications are commonplace and the challenges of treatment during pregnancy with few options available pose a risk to the health of both the mother and baby. Patients suffering from conditions such as preeclampsia, placenta accreta, and intrauterine growth restriction have few treatment options apart from emergency caesarean section. Fortunately, researchers are beginning to develop nanomedicine-based therapies that could be utilized to treat conditions affecting the mother, placenta, or fetus to improve the prognosis for mothers and their unborn children. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/d0tb00612bDOI Listing

Molecular and nanoengineering approaches towards activatable cancer immunotherapy.

Authors:
Chi Zhang Kanyi Pu

Chem Soc Rev 2020 May 26. Epub 2020 May 26.

School of Chemical and Biomedical Engineering, Nanyang Technological University, 70 Nanyang Drive, 637457, Singapore.

Cancer immunotherapy is an emerging treatment strategy that modulates the immune system to fight against cancer. Although several immunotherapeutic agents have been utilized in the clinic for cancer treatment, low patient response rates and potential immune-related adverse events remain two major challenges. With the merits of delivery controllability and modular flexibility, nanomedicines provide opportunities to facilitate immunotherapies for clinical translation in a safe and effective manner. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/c9cs00773cDOI Listing

Comparative study of α-helical and β-sheet self-assembled peptide nanofiber vaccine platforms: influence of integrated T-cell epitopes.

Biomater Sci 2020 May 26. Epub 2020 May 26.

Biomedical Engineering Department, Duke University, Durham, NC 27708, USA.

Several different self-assembling peptide systems that form nanofibers have been investigated as vaccine platforms, but design principles for adjusting the character of the immune responses they raise have yet to be well articulated. Here we compared the immune responses raised by two structurally dissimilar peptide nanofibers, one a β-sheet fibrillar system (Q11), and one an α-helical nanofiber system (Coil29), hypothesizing that integrated T-cell epitopes within the latter would promote T follicular helper (Tfh) cell engagement and lead to improved antibody titers and quality. Despite significantly different internal structures, nanofibers of the two peptides exhibited surprisingly similar nanoscale morphologies, and both were capable of raising strong antibody responses to conjugated peptide epitopes in mice without adjuvant. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1039/d0bm00521eDOI Listing