539 results match your criteria Bioengineered[Journal]


Optimization of N-hydroxysuccinimide ester coupling with aminoallyl-modified RNA for fluorescent labeling.

Authors:
Mengyang Li

Bioengineered 2020 Dec;11(1):599-606

State Key Laboratory of Microbial Metabolism, School of Life Science and Biotechnology, Shanghai Jiao Tong University , Shanghai, P. R. China.

Site-specific fluorescent labeling of RNA is crucial for obtaining the structural and dynamic information of RNAs by fluorescence techniques. Post-synthetic modification of RNA based on N-hydroxysuccinimide (NHS) coupling reaction is an economic, efficient and simple strategy to introduce fluorophore to samples. However, this strategy are not that frequently used in RNA molecules, and the reported reaction conditions and yields varied among different systems. Read More

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http://dx.doi.org/10.1080/21655979.2020.1765487DOI Listing
December 2020

From stale bread and brewers spent grain to a new food source using edible filamentous fungi.

Bioengineered 2020 Dec;11(1):582-598

Swedish Centre for Resource Recovery, University of Borås , Borås, Sweden.

By-products from the food sector with a high load of organic matter present both a waste-handling problem related to expenses and to the environment, yet also an opportunity. This study aims to increase the value of stale bread and brewers spent grain (BSG) by re-introducing these residues to the food production chain by converting them to new protein-enriched products using the edible filamentous fungi and . After 6 days of solid state fermentation (at 35°C, with a95% relative humidity and moisture content of 40% in the substrate) on stale bread, a nutrient-rich fungal-fermented product was produced. Read More

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http://dx.doi.org/10.1080/21655979.2020.1768694DOI Listing
December 2020

Role of bioengineering and laborers in integration of farmland resources toward to improve dimension of sustainable agriculture in China.

Bioengineered 2020 Dec;11(1):559-571

College of Natural Resources and Environment, Northwest A&F University, Yangling, P.R. China.

Farmland transfer is one of the essential approaches for achieving large-scale farming and its management affects productive efficiency, environment pollution and food sustainable security supply. Present study was carried out investigation based representative agricultural development area Guanzhong Plain of Shaanxi, aimed at explore the role of biotechnology and laborers in integration of farmland toward to improve sustainable agriculture in rural China by employed the profit and Tobit models evaluation. The conclusion demonstrated that labor's and agricultural management model as main stay, intensive farming has positive effect-based economic and environmental benefits than fragmentation management, female laborers have weaker effect on farmland renting-out behavior among smallholders while male laborers were superior promoters in increasing the area of rented-in farmland and farm scale. Read More

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http://dx.doi.org/10.1080/21655979.2020.1765523DOI Listing
December 2020

Dendritic cells in tumor microenvironment promoted the neuropathic pain via paracrine inflammatory and growth factors.

Bioengineered 2020 May 20. Epub 2020 May 20.

Department of Pain Management, Hospital Affiliated 5 to Nantong University (Taizhou people's Hospital) , Taizhou, 225300, China.

Neuropathic pain associated with cancers was caused by tumor itself or tumor therapy, which was aggravated by sensitizing nociceptor sensory neurons. The tumor microenvironment contributed to tumorigenesis, tumor progress, tumor metastasis, tumor immune resistance, tumor chemotherapy, and tumor immunotherapy. In the current study, we explored the contributions of the infiltrated dendritic cells insulted by Wnt1 in tumor microenvironment to neuropathic pain associated with cancers. Read More

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http://dx.doi.org/10.1080/21655979.2020.1771068DOI Listing

The clinicopathological and prognostic value of CD44 expression in bladder cancer: a study based on meta-analysis and TCGA data.

Bioengineered 2020 Dec;11(1):572-581

Department of Urology, The First Hospital of Jilin University, Changchun, Jilin, China.

CD44 is reported to be involved in tumor invasion and metastasis. However, the role of cancer stem cell marker CD44 in bladder cancer still remains controversial. Hence, the correlations between CD44 expression and the clinicopathological features and the prognosis of bladder cancer were investigated. Read More

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http://dx.doi.org/10.1080/21655979.2020.1765500DOI Listing
December 2020

miR-211 alleviates ischaemia/reperfusion-induced kidney injury by targeting TGFβR2/TGF-β/SMAD3 pathway.

Bioengineered 2020 Dec;11(1):547-557

Department of Anesthesia, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, China.

MicroRNA-211 (miR-211) is closely related to apoptosis and plays an important role in ischemia/reperfusion (I/R) injury. Whether miR-211 is involved in the protective effects in renal I/R injury is unknown. In this study, we evaluated the role of miR-211 in human tubular epithelial cells in response to hypoxia-reoxygenation (H/R) stimulation and I/R injury and . Read More

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http://dx.doi.org/10.1080/21655979.2020.1765501DOI Listing
December 2020

Valorizing agricultural biomass for sustainable development: biological engineering aspects.

Bioengineered 2020 Dec;11(1):522-523

College of Mechanical and Electrical Engineering, Henan Agricultural University, Zhengzhou, China.

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http://dx.doi.org/10.1080/21655979.2020.1759185DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7202690PMC
December 2020

Down-regulation of lncRNA DNAJC3-AS1 inhibits colon cancer via regulating miR-214-3p/LIVIN axis.

Bioengineered 2020 Dec;11(1):524-535

Department of General Surgery, Shengjing Hospital of China Medical University, Shenyang, Liaoning, People's Republic of China.

Long non-coding RNAs (lncRNAs) play a key role in the development and metastasis of cancer. However, the biological role and clinical significance of lncRNA DNAJC3-AS1 in the development of colon cancer is still unknown. In this study, the effects of DNAJC3-AS1 on cell proliferation, migration, and invasion were evaluated by MTT assay, wound-healing assay, and transwell assay, respectively. Read More

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http://dx.doi.org/10.1080/21655979.2020.1757224DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7202691PMC
December 2020

Long non-coding RNA H19 regulates proliferation and doxorubicin resistance in MCF-7 cells by targeting PARP1.

Bioengineered 2020 Dec;11(1):536-546

Department of Breast Surgery, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, China.

Chemoresistance is a major obstacle to effective breast cancer chemotherapy. However, the underlying molecular mechanisms remain unclear. The long noncoding RNA H19 (H19) is involved in various stages of tumorigenesis, however, its role in doxorubicin resistance remains unknown. Read More

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http://dx.doi.org/10.1080/21655979.2020.1761512DOI Listing
December 2020

Propofol inhibits migration and induces apoptosis of pancreatic cancer PANC-1 cells through miR-34a-mediated E-cadherin and LOC285194 signals.

Bioengineered 2020 Dec;11(1):510-521

Department of Anesthesia, Linyi Cancer Hospital, Linyi, Shandong, China.

Propofol has exhibited potent antitumor activity in pancreatic cancer cells and . The study aimed to investigate the anti-tumor mechanisms of propofol on pancreatic cancer PANC-1 cells . PANC-1 cells were exposure to concentration 20 μg/ml of propofol for 72 h. Read More

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http://dx.doi.org/10.1080/21655979.2020.1754038DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7185861PMC
December 2020

Effect of organic compounds on dry anaerobic digestion of food and paper industry wastes.

Bioengineered 2020 Dec;11(1):502-509

Swedish Centre for Resource Recovery, University of Borås, Borås, Sweden.

Effects of antimicrobial compounds on dry anaerobic digestion (dry-AD) processes were investigated. Four compounds with known inhibition effects on traditional wet digestion, car-3-ene, hexanal, 1-octanol and phenol were selected and investigated at concentrations of 0.005%, 0. Read More

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http://dx.doi.org/10.1080/21655979.2020.1752594DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7185885PMC
December 2020

Nucleus segmentation of cervical cytology images based on multi-scale fuzzy clustering algorithm.

Bioengineered 2020 Dec;11(1):484-501

School of Computer Science, Harbin University of Science and Technology, Harbin, China.

In the screening of cervical cancer cells, accurate identification and segmentation of nucleus in cell images is a key part in the early diagnosis of cervical cancer. Overlapping, uneven staining, poor contrast, and other reasons present challenges to cervical nucleus segmentation. We propose a segmentation method for cervical nuclei based on a multi-scale fuzzy clustering algorithm, which segments cervical cell clump images at different scales. Read More

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http://dx.doi.org/10.1080/21655979.2020.1747834DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7161549PMC
December 2020

Overexpression of secretory clusterin (sCLU) induces chemotherapy resistance in human gastric cancer cells by targeting miR-195-5p.

Bioengineered 2020 Dec;11(1):472-483

Department of General Surgery, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, China.

Recent focus has turned to secretory clusterin (sCLU) as a key contributor to chemoresistance of anticancer agents, but the role of sCLU on chemotherapy drug response to gastric cancer cells is not fully understood. Previous research found that sCLU was overexpressed in the induced multidrug-resistant MGC-803/5-FU cell line, suggesting that sCLU upregulation was closely related to chemoresistance to anticancer agents. In the present study, we aimed to clarify the role and mechanisms of sCLU in regulating the chemoresistance of gastric cancer cells. Read More

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http://dx.doi.org/10.1080/21655979.2020.1747825DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7161562PMC
December 2020

Nutrient supplementation strategy improves cell concentration and longevity, monoclonal antibody production and lactate metabolism of Chinese hamster ovary cells.

Bioengineered 2020 Dec;11(1):463-471

Programa de Investigación de Producción de Biomoléculas, Departamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Coyoacán, Ciudad De México, México.

A careful selection of culture mediums and feeds has become necessary to maximize yields of recombinant proteins during bioprocesses of mammalian cells. Supplements contain a variety of concentrate nutrients, and their beneficial effects vary according to recombinant cell lines. In this study, the effects of PowerFeed A on growth kinetics, productivity and cellular metabolism were evaluated for two Chinese hamster ovary cell lines producing a monoclonal antibody in a batch culture. Read More

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http://dx.doi.org/10.1080/21655979.2020.1744266DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7161567PMC
December 2020

Effect of histone demethylase KDM5A on the odontogenic differentiation of human dental pulp cells.

Bioengineered 2020 Dec;11(1):449-462

Guanghua School of Stomatology, Hospital of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-Sen University, Guangzhou, Guangdong, P.R. China.

Human dental pulp cells (hDPCs) possess the capacity to differentiate into odontoblast-like cells in response to exogenous stimuli. Histone methylation is one of the most robust epigenetic marks and is essential for the regulation of multiple cellular processes. Previous studies have shown that histone methyltransferases (HMTs) and histone demethylases (HDMs) are crucial for the osteogenic differentiation of human bone marrow, adipose tissue, and tooth tissue. Read More

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http://dx.doi.org/10.1080/21655979.2020.1743536DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7161540PMC
December 2020

Process optimization for the anaerobic digestion of poplar (.) leaves.

Bioengineered 2020 12;11(1):439-448

Key Laboratory of Renewable Energy of Ministry of Agriculture, Henan Agricultural University/Collaborative Innovation Center of Biomass Energy, Zhengzhou, China.

This study investigates the optimized condition for enhancing biogas production in the anaerobic digestion of fallen poplar leaves. Two experiments were conducted: (1) The calcium hydroxide concentration, bacteria concentration, and composting time were used as three parameters to optimize the fermentation pretreatment condition and contrasting tests were performed; and (2) a series of fermentation tests were conducted to explore the best process parameters and biogas production characteristics. The results showed that a biological and chemical combined pretreatment effectively improved the biogas productivity of poplar leaves as fermentation substrates, and the parameter that had the greatest effect during anaerobic digestion was temperature followed by the solid concentration and pH value. Read More

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http://dx.doi.org/10.1080/21655979.2020.1739823DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7161560PMC
December 2020

Statistical optimization of simultaneous saccharification fermentative hydrogen production from corn stover.

Bioengineered 2020 12;11(1):428-438

Key Laboratory of New Materials and Facilities for Rural Renewable Energy Ministry of Agriculture, Henan Agricultural University, Zhengzhou, China.

Corn stovers are rich in carbohydrates and can be used by anaerobic bacteria to produce hydrogen by fermentation. In the present study, using hydrogen production as the main experimental index, the effect of different influential factors on hydrogen production from corn stover saccharification and fermentation was studied, using the response surface method BBD model. The significance of interactions between different influential factors on hydrogen production by simultaneous saccharification and fermentation of corn stover material were investigated and optimized. Read More

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http://dx.doi.org/10.1080/21655979.2020.1739405DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7161564PMC
December 2020

Fatty acid methyl ester analysis of isolated from fruit pulps for biodiesel production using GC-MS spectrometry.

Bioengineered 2020 12;11(1):408-415

Department of Bioengineering, Faculty of Chemical and Metallurgical Engineering, Yildiz Technical University, Istanbul, Turkey.

In this study, a total of six fungal samples were isolated from apple, strawberry and orange pulp. DNA sequence analysis was used as molecular identification method. ITS region was aligned in DNA sequence analysis, and an algorithm sequence similarity was done using BLAST (Basic Local Alignment Search Tool) program to identify these isolates. Read More

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http://dx.doi.org/10.1080/21655979.2020.1739379DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7161539PMC
December 2020

Biocatalytic synthesis of lactosucrose using a recombinant thermostable β-fructofuranosidase from sp. 10138.

Bioengineered 2020 12;11(1):416-427

Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi, China.

As a prebiotics, lactosucrose plays an important role in maintaining human gastrointestinal homeostasis. In this study, a thermostable enzyme from sp. 10138 was screened from six β-fructofuranosidase-producing strains for the lactosucrose production and the coding gene was heterologously expressed in for efficient expression. Read More

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http://dx.doi.org/10.1080/21655979.2020.1739404DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7161541PMC
December 2020

Enhancement on enzymolysis of pigskin with ultrasonic assistance.

Bioengineered 2020 12;11(1):397-407

Key Laboratory of New Materials and Facilities for Rural Renewable Energy of China's Ministry of Agriculture and Rural Affairs, College of Mechanical & Electrical Engineering, Henan Agricultural University, Zhengzhou, China.

The fur is hard to decompose during the fermentation process of diseased swine carcasses. In order to enhance the enzymolysis of pigskin, the ultrasonic was proposed to use during the process of the enzymatic hydrolysis. The response surface optimization experiments were carried out with the DH (degree of hydrolysis) as the response value and the optimum conditions for enzymatic hydrolysis were determined. Read More

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http://dx.doi.org/10.1080/21655979.2020.1736736DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7170554PMC
December 2020

Improved production of recombinant lipase by coexpressing protein folding chaperones in , which triggered ER stress.

Bioengineered 2020 12;11(1):375-385

State Key Laboratory of Agrobiotechnology and MOA Key Laboratory of Soil Microbiology, College of Biological Sciences, China Agricultural University, Beijing, China.

lipase (RML) is a biocatalyst that widely used in laboratory and industrial. Previously, RML with a 70-amino acid propeptide (pRML) was cloned and expressed in . Recombinant strains with (strain containing 4-copy ) and without ER stress (strain containing 2-copy ) were obtained. Read More

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http://dx.doi.org/10.1080/21655979.2020.1738127DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7161542PMC
December 2020

Rapid propagation in vitro and accumulation of active substances of endangered Rchb. f.

Bioengineered 2020 12;11(1):386-396

Bioresource Institute for Healthy Utilization, Zunyi Medical University, Zunyi, China.

is a valuable ornamental and medicinal plant rich with polysaccharides, alkaloid, and other bioactive compounds, which are potential raw materials for pharmacological utilization. In this study, an efficient protocol for the rapid propagation of was developed. By using the tissue culture protocol, the effects of pH, hormone combinations, temperatures, light intensity, culture time protocorm proliferation, seedlings rooting, and accumulation of biomass with bioactive compounds were investigated. Read More

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http://dx.doi.org/10.1080/21655979.2020.1739406DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7161565PMC
December 2020

Expression of chromogranin A-derived antifungal peptide CGA-N12 in .

Bioengineered 2020 12;11(1):318-327

Department of Respiratory Medicine, Affiliated Fuzhou First Hospital of Fujian Medical University, Fuzhou, Fujian, China.

The human chromogranin A-derived peptide CGA-N12, which is composed of 12 amino acid residues with the sequence ALQGAKERAHQQ, showed strong antifungal activity and the least hemolytic activity in previous studies. However, synthetic peptides are relatively expensive to produce. Recombinant expression of peptides in the host cells, such as bacteria or yeast, can fastly provide cost-efficient products of peptides. Read More

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http://dx.doi.org/10.1080/21655979.2020.1736237DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7161563PMC
December 2020

Metagenomics for taxonomy profiling: tools and approaches.

Bioengineered 2020 12;11(1):356-374

College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi Province, China.

The study of metagenomics is an emerging field that identifies the total genetic materials in an organism along with the set of all genetic materials like deoxyribonucleic acid and ribose nucleic acid, which play a key role with the maintenance of cellular functions. The best part of this technology is that it gives more flexibility to environmental microbiologists to instantly pioneer the immense genetic variability of microbial communities. However, it is intensively complex to identify the suitable sequencing measures of any specific gene that can exclusively indicate the involvement of microbial metagenomes and be able to advance valuable results about these communities. Read More

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http://dx.doi.org/10.1080/21655979.2020.1736238DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7161568PMC
December 2020

Advanced in developmental organic and inorganic nanomaterial: a review.

Bioengineered 2020 12;11(1):328-355

Nanotechnology & Catalysis Research Centre (NANOCAT), University of Malaya (UM), Kuala Lumpur, Malaysia.

With the unique properties such as high surface area to volume ratio, stability, inertness, ease of functionalization, as well as novel optical, electrical, and magnetic behaviors, nanomaterials have a wide range of applications in various fields with the common types including nanotubes, dendrimers, quantum dots, and fullerenes. With the aim of providing useful insights to help future development of efficient and commercially viable technology for large-scale production, this review focused on the science and applications of inorganic and organic nanomaterials, emphasizing on their synthesis, processing, characterization, and applications on different fields. The applications of nanomaterials on imaging, cell and gene delivery, biosensor, cancer treatment, therapy, and others were discussed in depth. Read More

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http://dx.doi.org/10.1080/21655979.2020.1736240DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7161543PMC
December 2020

Combined freezing-thawing pretreatment and microbial electrolysis cell for enhancement of highly concentrated organics degradation from dewatered sludge.

Bioengineered 2020 12;11(1):301-310

Hydrology and Water Resources Bureau of Henan Province, Zhengzhou, P.R. China.

The influence of freezing-thawing (F/T) pretreatment on the degradation of highly concentrated organic matters from dewatered sludge (DS) in microbial electrolysis cell (MEC) was investigated in this study. Extended freezing disintegrated the DS matrix and resulted in accelerated hydrolysis rate. The biogas production and stabilization were increased due to the pretreatment by 25-70% of H production rate and 17. Read More

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http://dx.doi.org/10.1080/21655979.2020.1736735DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7161561PMC
December 2020

Enhancement of pH values stability and photo-fermentation biohydrogen production by phosphate buffer.

Bioengineered 2020 12;11(1):291-300

Collaborative Innovation Center of Biomass Energy, Henan Agricultural University, Zhengzhou, China.

The main aim of this study was to investigate the effects of the initial pH values of the buffer on photo-fermentation biohydrogen production. Hydrogen production and the kinetics of it under different initial pH values were analyzed. Effects of initial pH values on reducing sugar consumption, hydrogen production rate, and byproduct production were evaluated at initial pH values of 5-7. Read More

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http://dx.doi.org/10.1080/21655979.2020.1736239DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7161566PMC
December 2020

Biological pretreatment of corn straw for enhancing degradation efficiency and biogas production.

Bioengineered 2020 12;11(1):251-260

Key Laboratory of New Materials and Facilities for Rural Renewable Energy, Ministry of Agriculture, College of Mechanical & Electrical Engineering, Henan Agricultural University, Zhengzhou, China.

In order to explore the effect of pretreatment on corn straw degradation and biogas production, corn straw was pretreated with mixed microbes and composting at 30°C for 14 days. The characteristics of material were measured and analyzed in the pretreatment process. Then, the pretreated and untreated corn straw was digested by anaerobic fermentation. Read More

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http://dx.doi.org/10.1080/21655979.2020.1733733DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7161559PMC
December 2020

Optimization of extraction of loquat flowers polyphenolics and its antioxidant and anti-polyphenol oxidase properties.

Bioengineered 2020 12;11(1):281-290

Agricultural Product Storage and Processing Laboratory, Suzhou Academy of Agricultural Sciences, Suzhou, China.

In this study, the conditions of extraction of loquat flowers polyphenolics were optimized through response surface methodology (RSM). Proper extraction conditions were: solid to liquid ratio 1 g per 50 mL and ethanol concentration 50% at 61°C for 9 min. Furthermore, the antioxidant and anti-polyphenol oxidase (PPO) activity of purified total polyphenolics (PTP) were investigated. Read More

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http://dx.doi.org/10.1080/21655979.2020.1735604DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7161536PMC
December 2020

Serum miR-125b levels associated with epithelial ovarian cancer (EOC) development and treatment responses.

Bioengineered 2020 12;11(1):311-317

Department of Gynecology, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, China.

Downexpression of miRs was associated with tumor development, progression, and metastasis. This study explored the serum levels of miR-125b in patients with epithelial ovarian cancer (EOC) and to assess its diagnostic value and monitor treatment responses for patients with EOC. A total of 379 individuals were recruited and assigned to the study groups. Read More

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http://dx.doi.org/10.1080/21655979.2020.1736755DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7161537PMC
December 2020

Glucose fermentation with biochar-amended consortium: microbial consortium shift.

Bioengineered 2020 12;11(1):272-280

Department of Chemical Engineering, National Taiwan University, Taipei, Taiwan.

The effects of adding biochar rice husk (R), white popinee (WP), bamboo (BB), or coconut (CT) on microbial community in fermentation broths from glucose were investigated. The added biochars acted as biofilm carriers on which 11 sp., 12 sp. Read More

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http://dx.doi.org/10.1080/21655979.2020.1735668DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7161558PMC
December 2020

FAM136A immunoreactivity is associated with nodal involvement and survival in lung adenocarcinoma in a Chinese case series.

Bioengineered 2020 12;11(1):261-271

Department of Thoracic Surgery, The Third Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, P.R. China.

Lung cancer patients with lymph node metastasis usually had short overall survival and occurred distant metastases at the early stage. However, some of these people did have more prolonged survival. The underlying reason is still unclear. Read More

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http://dx.doi.org/10.1080/21655979.2020.1735611DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7051133PMC
December 2020

Ultrasound-assisted hydrolysis of lard for free fatty acids catalyzed by combined two lipases in aqueous medium.

Bioengineered 2020 12;11(1):241-250

Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, School of Life Sciences, Jiangsu Normal University, Xuzhou, P. R. China.

Lard is a by-product of animal processing. It is inexpensive compared with vegetable oils; however, its use is limited due to the high calorific value and high-saturated fatty acid content. While using lard as the source of free fatty acids (FFA) can significantly increase its utilization value. Read More

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http://dx.doi.org/10.1080/21655979.2020.1729678DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7039637PMC
December 2020

MicroRNA-145 overexpression inhibits neuroblastoma tumorigenesis and .

Bioengineered 2020 12;11(1):219-228

Department of Pediatric Surgery, Hospital of Qingdao University, Qingdao, Shandong, China.

Neuroblastoma (NB) is responsible for 15% of all childhood cancer deaths. Despite advances in treatment and disease management, the overall 5-year survival rates remain poor in high-risk disease (25-40%). It is well known that miR-145 functions as a tumor suppressor in several types of cancer. Read More

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http://dx.doi.org/10.1080/21655979.2020.1729928DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7039631PMC
December 2020

Special issue on algae bioprocess engineering.

Bioengineered 2020 12;11(1):188

National Cheng Kung University, Tainan City,

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http://dx.doi.org/10.1080/21655979.2020.1729546DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7039636PMC
December 2020

miR-22 protect PC12 from ischemia/reperfusion-induced injury by targeting p53 upregulated modulator of apoptosis (PUMA).

Bioengineered 2020 12;11(1):209-218

Department of Anesthesia, Linyi Cancer Hospital, Linyi, Shandong, China.

MicroRNAs have been implicated as critical regulatory molecules in many cerebrovascular diseases. Recent studies demonstrated miR-22 might provide a potential neuroprotective effect. However, the neuroprotective effect of miR-22 in ischemia/reperfusion (I/R) injury has not been thoroughly elucidated. Read More

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http://dx.doi.org/10.1080/21655979.2020.1729321DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7039629PMC
December 2020

Rhizoremediation of Cu(II) ions from contaminated soil using plant growth promoting bacteria: an outlook on pyrolysis conditions on plant residues for methylene orange dye biosorption.

Bioengineered 2020 12;11(1):175-187

Department of Biotechnology, Rajalakshmi Engineering College, Chennai, India.

Rhizoremediation is one of the most accepted, cost-effective bioremediation techniques focusing on the application of rhizospheric microorganisms in combination with plants for the remediation of organic and inorganic pollutants from the contaminated sites. This work focuses on isolation and identification of metal resistant bacteria to grow on medium with the copper ion concentration of 1500 mg/L. The resistant isolate was identified as by a 16S rRNA sequencing. Read More

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http://dx.doi.org/10.1080/21655979.2020.1728034DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7039635PMC
December 2020

COTI-2 induces cell apoptosis in pediatric acute lymphoblastic leukemia via upregulation of miR-203.

Bioengineered 2020 12;11(1):201-208

Department of Clinical Laboratory, Qingdao Women and Children's Hospital, Qingdao, Shandong, China.

COTI-2 is a third-generation thiosemicarbazone, which is effective against a diverse group of human cancer cell lines at nanomolar concentrations. COTI-2 also showed superior activity against tumor cells, in vitro and in vivo. As a high efficacy and low toxicity agent, it currently candidates in a phase I clinical study of gynecological malignancies and head and neck squamous cell carcinoma (HNSCC). Read More

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http://dx.doi.org/10.1080/21655979.2020.1729927DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7039633PMC
December 2020

Dominant protozoan species in rhizosphere soil over growth of . in Northeast China.

Bioengineered 2020 12;11(1):229-240

Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei, Taiwan.

This paper identified the dominant protozoan species in the four layers of rhizosphere soil during the six growth stages of . and analyzed the correlations of the abundance and diversity of the dominant protozoan species with soil properties at different growth stages and soil depth. A total of 15 species of protozoa were identified; among them, sp. Read More

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http://dx.doi.org/10.1080/21655979.2020.1729929DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7039641PMC
December 2020

MiR-211 protects cerebral ischemia/reperfusion injury by inhibiting cell apoptosis.

Bioengineered 2020 12;11(1):189-200

Department of Anesthesia, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, China.

MicroRNAs (miRNAs) have emerged as critical regulators of neuronal survival during cerebral ischemia/reperfusion injury. Accumulating evidence has shown that miR-211 plays a crucial role in regulating apoptosis and survival in various cell types. However, whether miR-211 is involved in regulating neuronal survival during cerebral ischemia/reperfusion injury remains unknown. Read More

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http://dx.doi.org/10.1080/21655979.2020.1729322DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7039642PMC
December 2020

Development of a 15-gene signature for predicting prognosis in advanced colorectal cancer.

Bioengineered 2020 12;11(1):165-174

Department of Anesthesiology, Beijing Shijitan Hospital, Capital Medical University, Beijing, Haidian, China.

Advanced colorectal cancer (CRC) is a significant cause of cancer mortality, with a poor prognosis. Here, we identified a novel prognostic signature for predicting survival of advanced CRC. Advanced CRC data were used (training set: n = 267 and validation set: n = 264). Read More

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http://dx.doi.org/10.1080/21655979.2020.1718459DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7039649PMC
December 2020

Estimation of carbon dioxide (CO) reduction by utilization of algal biomass bioplastic in Malaysia using carbon emission pinch analysis (CEPA).

Bioengineered 2020 12;11(1):154-164

Department of Chemical and Environment Engineering, Faculty of Science and Engineering, University of Nottingham Malaysia, Semenyih, Malaysia.

Carbon dioxide (CO) emission will increase due to the increasing global plastic demand. Statistical data shows that plastic production alone will contribute to at least 20% of the annual global carbon budget in the near future. Hence, several alternative methods are recommended to overcome this problem, such as bio-product synthesis. Read More

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http://dx.doi.org/10.1080/21655979.2020.1718471DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6999637PMC
December 2020

Impact of magnetic immobilization on the cell physiology of green unicellular algae .

Bioengineered 2020 12;11(1):141-153

Department of Medical Nanotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran.

Cell immobilization on the magnetic nanoparticles (MNPs) and magnetic harvesting is a novel approach for microalgal cells separation. To date, the effect of these nanoparticles on microalgal cells was only studied over a short period of time. More studies are hence needed for a better understanding of the magnetic harvesting proposes or environmental concerns relating to long-term exposure to nanoparticles. Read More

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http://dx.doi.org/10.1080/21655979.2020.1718477DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6999624PMC
December 2020

Targeting survivin sensitizes cervical cancer cells to radiation treatment.

Bioengineered 2020 12;11(1):130-140

Department of Radiotherapy, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, China.

Survivin is an inhibitor of apoptosis protein that functions to inhibit apoptosis, promote proliferation, and enhance invasion. It is selectively up-regulated in many human tumors and implicated in cellular radiation response through its role in apoptosis, cell division, and DNA damage response. This study aimed to investigate the effect and mechanisms of targeting survivin radiosensitivity in cervical cancer C33A cells. Read More

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http://dx.doi.org/10.1080/21655979.2020.1717297DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6984589PMC
December 2020

miR-25-3p inhibition impairs tumorigenesis and invasion in gastric cancer cells and .

Bioengineered 2020 12;11(1):81-90

Department of General Surgery, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, China.

Deregulated expression of microRNAs (miRNAs) plays a role in the pathogenesis and progression of gastric cancer (GC). Among upregulated miRNAs, miR-25-3p has oncogenic potential and therefore represents an attractive target for the treatment of GC. Here, we investigated the role of miR-25-3p on GC cells and . Read More

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http://dx.doi.org/10.1080/21655979.2019.1710924DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6961587PMC
December 2020

A review on microalgae cultivation and harvesting, and their biomass extraction processing using ionic liquids.

Bioengineered 2020 12;11(1):116-129

Department of Chemical and Environmental Engineering, Faculty of Science and Engineering, University of Nottingham Malaysia, Semenyih, Malaysia.

The richness of high-value bio-compounds derived from microalgae has made microalgae a promising and sustainable source of useful product. The present work starts with a review on the usage of open pond and photobioreactor in culturing various microalgae strains, followed by an in-depth evaluation on the common harvesting techniques used to collect microalgae from culture medium. The harvesting methods discussed include filtration, centrifugation, flocculation, and flotation. Read More

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http://dx.doi.org/10.1080/21655979.2020.1711626DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6999644PMC
December 2020

MiR-132 inhibits migration and invasion and increases chemosensitivity of cisplatin-resistant oral squamous cell carcinoma cells via targeting TGF-β1.

Bioengineered 2020 12;11(1):91-102

Department of Oral and Maxillofacial Surgery, The affiliated Hospital of Qingdao University, Qingdao, Shandong, China.

Numerous findings have demonstrated that MicroRNAs dysregulation plays a key role in many neoplasms, including oral squamous cell carcinoma (OSCC), yet the potential mechanisms of microRNAs in chemo-resistance remain elusive. Here, we analyzed the miR-132 expression in OSCC tissues and OSCC cell lines, and explored it role and mechanisms on invasion and migration and cisplatin (CDDP)-induced cell death. The clinical tissues of 37 patients with OSCCs and paired normal tissues were collected. Read More

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http://dx.doi.org/10.1080/21655979.2019.1710925DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6961592PMC
December 2020

Genome-wide analysis of family in .

Bioengineered 2020 12;11(1):103-115

State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, China.

Abscisic acid (ABA) signaling regulates plant growth and development and participates in response to abiotic stressors. However, details about the gene family, which is the core component of ABA signaling in , are unknown. In this work, we identified 14 pyrabactin resistance-likes (), 84 type 2C protein phosphatase (), and 8 SNF1-related protein kinase 2s () from . Read More

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http://dx.doi.org/10.1080/21655979.2019.1710932DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6961588PMC
December 2020

MiR-187 suppresses non-small-cell lung cancer cell proliferation by targeting FGF9.

Bioengineered 2020 12;11(1):70-80

Department of Respiratory, HeXian Memorial Hospital Affiliated with Southern Medical University, Guang zhou, China.

Non-small-cell lung cancer (NSCLC) is the main pathological type of lung cancer and has a low overall five-year survival rate. miR-187 has been reported to play major roles in various tumor types. In this study, we explored the impact of miR-187 on NSCLC. Read More

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http://dx.doi.org/10.1080/21655979.2019.1706287DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6961586PMC
December 2020