431 results match your criteria Bioengineered[Journal]


Examining the molecular characteristics of glycoside hydrolase family 20 β-N-acetylglucosaminidases with high activity.

Bioengineered 2019 Dec;10(1):71-77

a Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education , Yunnan Normal University , Kunming , P. R. China.

β-N-Acetylglucosaminidases (GlcNAcases) possess many important biological functions and are used for promising applications that are often hampered by low-activity enzymes. We previously demonstrated that most GlcNAcases of the glycoside hydrolase (GH) family 20 showed higher activities than those of other GH families, and we presented two novel GH 20 GlcNAcases that showed higher activities than most GlcNAcases. A highly flexible structure, which was attributed to the presence of to a high proportion of random coils and flexible amino acid residues, was presumed to be a factor in the high activity of GH 20 GlcNAcases. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/21655979.2019.1602427DOI Listing
December 2019
1 Read

Targeting MIAT reduces apoptosis of cardiomyocytes after ischemia/reperfusion injury.

Bioengineered 2019 Apr 11. Epub 2019 Apr 11.

b Department of Anesthesia , the affiliated hospital of Qingdao University , Qingdao Shandong, China .

This study aims to investigate the role of targeting lncRNA myocardial infarction-associated transcript (MIAT) in protection against hypoxia/reoxygenation (H/R) injury in H9c2 cells in vitro and myocardial ischemia/reperfusion (I/R) injury in vivo by regulating expression of NF-kB and p53 upregulated modulator of apoptosis (PUMA). H9C2 cells were infected with lentivirus expressing the short-hairpin RNA direct against human MIAT gene (Lv-MIAT shRNA) or lentivirus expressing scrambled control (Lv-NC shRNA) or PUMA siRNA or p65 siRNA or their control siRNA respectively. Then the H9c2 cells were infected with Lv-shRNA to 2 hours of hypoxia (H) and 24 hour of reoxygenation (R). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/21655979.2019.1605812DOI Listing
April 2019
1 Read

Enhanced secretion of human α1-antitrypsin expressed with a novel glycosylation module in tobacco BY-2 cell culture.

Bioengineered 2019 Dec;10(1):87-97

a Arkansas Biosciences Institute , Jonesboro , AR , USA.

Expression of recombinant proteins fused to a novel glycomodule tag, termed hydroxyproline (Hyp)-O-glycosylated peptides (HypGP), was earlier found to boost secreted protein yields up to 500-fold in plant cell culture. Here, this technology was applied to the expression of human protease inhibitor α1-antitrypsin (AAT) in tobacco BY-2 cell culture. A designer HypGP tag composed of a 'Ala-Pro' motif of 20 units, or (AP), was engineered either at the N- or C-terminal end of AAT. Read More

View Article

Download full-text PDF

Source
https://www.tandfonline.com/doi/full/10.1080/21655979.2019.1
Publisher Site
http://dx.doi.org/10.1080/21655979.2019.1604037DOI Listing
December 2019
1 Read

Hydrolysing the soluble protein secreted by Escherichia coli in trans-4-hydroxy-L-proline fermentation increased dissolve oxygen to promote high-level trans-4-hydroxy-L-proline production.

Authors:
Xiaocui Liu

Bioengineered 2019 Dec;10(1):52-58

a School of Life Sciences of Shanxi Datong University , Datong Shanxi , China.

Trans-4-hydroxy-L-proline (Hyp) production by Escherichia coli (E. coli) in fermentation is a high-oxygen-demand process. E. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/21655979.2019.1600966DOI Listing
December 2019
1 Read

Adaptation effects of medial forebrain bundle micro-electrical stimulation.

Bioengineered 2019 Dec;10(1):78-86

a Neuroscience & Neuroengineering Research Lab., Biomedical Engineering Department , School of Electrical Engineering, Iran University of Science and Technology (IUST) , Tehran , Iran.

Brain micro-electrical stimulation and its applications are among the most important issues in the field of brain science and neurophysiology. Deep brain stimulation techniques have been used in different theraputic or alternative medicine applications including chronic pain control, tremor control, Parkinson's disease control and depression control. Recently, brain electrical stimulation has been used for tele-control and navigation of small animals such as rodents and birds. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/21655979.2019.1599628DOI Listing
December 2019

Application of an immunoglobulin Y-alkaline phosphatase bioconjugate as a diagnostic tool for influenza A virus.

Bioengineered 2019 Dec;10(1):33-42

c GlaxoSmithKline-Vaccine , Istanbul , Turkey.

The diagnosis of influenza A virus is essential since it can be confused with influenza A like illness and lead to inaccurate drug prescription. In this study, the M2e peptide, a strategic antigen that is conserved in all virus subtypes, was used as a diagnostic marker of influenza A. For the first time, M2e-specific IgY antibody was covalently conjugated to alkaline phosphatase (ALP) enzyme in the presence of glutaraldehyde. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/21655979.2019.1586054DOI Listing
December 2019

Utilization of acid hydrolysate of recovered bacterial cell as a novel organic nitrogen source for L-tryptophan fermentation.

Bioengineered 2019 Dec;10(1):23-32

b State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy, Tianjin Key Laboratory of Molecular Drug Research , Nankai University , Tianjin , China.

In this study, waste bacterial cell (WBC) was recovered and used as an alternative to yeast extract in L-tryptophan fermentation. The effects of sulfuric acid concentration and temperature on the hydrolysis of WBC were optimized and the amino acid content in the waste bacterial cell hydrolysate (WBCH) was increased. Plackett-Burman and Box-Behnken design analysis revealed the optimum composition of the WBCH-based fermentation medium to be 22. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/21655979.2019.1586053DOI Listing
December 2019

Engineering of L-amino acid deaminases for the production of α-keto acids from L-amino acids.

Bioengineered 2019 Dec;10(1):43-51

a Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education , Jiangnan University , Wuxi , China.

α-keto acids are organic compounds that contain an acid group and a ketone group. L-amino acid deaminases are enzymes that catalyze the oxidative deamination of amino acids for the formation of their corresponding α-keto acids and ammonia. α-keto acids are synthesized industrially via chemical processes that are costly and use harsh chemicals. Read More

View Article

Download full-text PDF

Source
https://www.tandfonline.com/doi/full/10.1080/21655979.2019.1
Publisher Site
http://dx.doi.org/10.1080/21655979.2019.1595990DOI Listing
December 2019
1 Read

Central metabolic pathway modification to improve L-tryptophan production in Escherichia coli.

Bioengineered 2019 Dec;10(1):59-70

a College of Biotechnology , Tianjin University of Science and Technology , Tianjin , China.

Tryptophan, an aromatic amino acid, has been widely used in food industry because it participates in the regulation of protein synthesis and metabolic network in vivo. In this study, we obtained a strain named TRP03 by enhancing the tryptophan synthesis pathway, which could accumulate tryptophan at approximately 35 g/L in a 5 L bioreactor. We then modified the central metabolic pathway of TRP03, to increase the supply of the precursor phosphoenolpyruvate (PEP), the genes related to PEP were modified. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/21655979.2019.1592417DOI Listing
December 2019

MicroRNA-485-5p suppresses the proliferation, migration and invasion of small cell lung cancer cells by targeting flotillin-2.

Bioengineered 2019 Dec;10(1):1-12

a Department of Thoracic Surgery , the Fourth Hospital of Hebei Medical University , Shijiazhuang , China.

This study is aimed to elucidate the mechanisms underlying the role of miR-485-5p in small cell lung cancer (SCLC). The expression of miR-485-5p were quantified with real time quantitative PCR and it was found that the level of miR-485-5p was lower in SCLC tissues than normal tissues. In cultured SCLC cell lines, overexpression of miR-485-5p reduced cell proliferation, migration, and invasion in vitro, whereas knockdown of miR-485-5p performed contrary. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/21655979.2019.1586056DOI Listing
December 2019
1 Read

Isolation of a novel deep-sea Bacillus circulus strain and uniform design for optimization of its anti-aflatoxigenic bioactive metabolites production.

Bioengineered 2019 Dec;10(1):13-22

a School of Marine Science and Technology , Harbin Institute of Technology , Weihai , Shandong , China.

The deep-sea bacterium strain FA13 was isolated from the sediment of the South Atlantic Ocean and identified as Bacillus circulans based on 16S ribosomal DNA sequence. Through liquid fermentation with five media, the cell-free supernatant fermented with ISP2 showed the highest inhibition activities against mycelial growth of Aspergillus parasiticus mutant strain NFRI-95 and accumulation of norsolorinic acid, a precursor for aflatoxin production. Based on ISP2, uniform design was used to optimize medium formula and fermentation conditions. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/21655979.2019.1586055DOI Listing
December 2019
1 Read

Prediction of RNA-protein interactions using conjoint triad feature and chaos game representation.

Bioengineered 2018 ;9(1):242-251

b College of Informatics , Huazhong Agricultural University , Wuhan P.R. of China.

RNA-protein interactions (RPIs) play a very important role in a wide range of post-transcriptional regulations, and identifying whether a given RNA-protein pair can form interactions or not is a vital prerequisite for dissecting the regulatory mechanisms of functional RNAs. Currently, expensive and time-consuming biological assays can only determine a very small portion of all RPIs, which calls for computational approaches to help biologists efficiently and correctly find candidate RPIs. Here, we integrated a successful computing algorithm, conjoint triad feature (CTF), and another method, chaos game representation (CGR), for representing RNA-protein pairs and by doing so developed a prediction model based on these representations and random forest (RF) classifiers. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/21655979.2018.1470721DOI Listing
March 2019
9 Reads

Decolorization of mordant yellow 1 using Aspergillus sp. TS-A CGMCC 12964 by biosorption and biodegradation.

Bioengineered 2018 ;9(1):222-232

a Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan/School of Chemistry and Chemical Engineering , Shihezi University , Shihezi , PR China.

In this report, the decolorization features of extracellular enzymes and mycelia separately prepared from Aspergillus sp. TS-A CGMCC 12,964 (120 h) were investigated. The fermentation broth of TS-A degraded 98. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/21655979.2018.1472465DOI Listing
March 2019
4 Reads

CRISPR-Cas systems: ushering in the new genome editing era.

Bioengineered 2018 ;9(1):214-221

a Centre for Plant Genetics and Breeding, School of Agriculture and Environment , The University of Western Australia , Crawley , WA , Australia.

In recent years there has been great progress with the implementation and utilization of Clustered Regularly Interspaced Palindromic Repeats (CRISPR) and CRISPR-associated protein (Cas) systems in the world of genetic engineering. Many forms of CRISPR-Cas9 have been developed as genome editing tools and techniques and, most recently, several non-genome editing CRISPR-Cas systems have emerged. Most of the CRISPR-Cas systems have been classified as either Class I or Class II and are further divided among several subtypes within each class. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/21655979.2018.1470720DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6067892PMC

Biosynthesis of ethylene glycol from d-xylose in recombinant Escherichia coli.

Bioengineered 2018 ;9(1):233-241

a CAS Key Laboratory of Biobased Materials , Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences , Qingdao , China.

Ethylene glycol (EG) is an important chemical used as antifreeze and a raw material in polyester synthesis. The EG biosynthetic pathway from D-xylose with D-xylonate as key intermediate has some advantages, but showed low EG production. Here, we reconstructed and optimized this pathway in Escherichia coli. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/21655979.2018.1478489DOI Listing
March 2019
3 Reads

Engineering NOG-pathway in Escherichia coli for poly-(3-hydroxybutyrate) production from low cost carbon sources.

Bioengineered 2018 01;9(1):209-213

a Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences , Tianjin , China.

Poly-(3-hydroxybutyrate) (P3HB) is a polyester with biodegradable and biocompatible characteristics suitable for bio-plastics and bio-medical use. In order to reduce the raw material cost, cheaper carbon sources such as xylose and glycerol were evaluated for P3HB production. We first conducted genome-scale metabolic network analysis to find the optimal pathways for P3HB production using xylose or glycerol respectively as the sole carbon sources. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/21655979.2018.1467652DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5972911PMC
January 2018
2 Reads

Mycobacterium tuberculosis H37Rv infection regulates alternative splicing in Macrophages.

Bioengineered 2018 01;9(1):203-208

d Institute of Biomedical Sciences, Fudan University , Shanghai , China.

Objective: The objective of this study was to evaluate the expression of genes encoding SR proteinsand alternative splicing of IL4 and TLR4 in Mycobacterium tuberculosis (M. tb) H37Rv-infected macrophages.

Materials And Methods: THP-1 cells were induced to differentiate into macrophages with 200 nM PMA, and H37Rv strains were used for macrophage infection. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/21655979.2017.1387692DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5972922PMC
January 2018
2 Reads

A Gene selection approach based on the fisher linear discriminant and the neighborhood rough set.

Bioengineered 2018 Jan 19;9(1):144-151. Epub 2017 Dec 19.

a College of Computer & Information Engineering, Henan Normal University , Xinxiang , Henan , China.

In recent years, tumor classification based on gene expression profiles has drawn great attention, and related research results have been widely applied to the clinical diagnosis of major gene diseases. These studies are of tremendous importance for accurate cancer diagnosis and subtype recognition. However, the microarray data of gene expression profiles have small samples, high dimensionality, large noise and data redundancy. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/21655979.2017.1403678DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5972918PMC
January 2018
6 Reads

So long, farewell, Auf Wiedersehen, Adieu….

Authors:
Roy D Sleator

Bioengineered 2018 01 30;9(1). Epub 2017 Nov 30.

a Department of Biological Sciences , Cork Institute of Technology , Bishopstown , Cork , Ireland.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/21655979.2017.1404788DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5972926PMC
January 2018
2 Reads

Graphene based scaffolds on bone tissue engineering.

Bioengineered 2018 01 30;9(1):38-47. Epub 2017 Nov 30.

d Stem Cell Research Center, Tabriz University of Medical Sciences , Tabriz , Iran.

Tissue engineering has been emerging as a valid approach to the current therapies for bone regeneration/substitution. Tissue-engineered bone constructs have the potential to alleviate the demand arising from the shortage of suitable autograft and allograft materials for augmenting bone healing. Scaffolds play a central role in tissue engineering research, they not only provide as structural support for specific cells but also provide as the templates to guide new tissue growth and construction. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/21655979.2017.1373539DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5972914PMC
January 2018
1 Read

In silico design of a novel chimeric shigella IpaB fused to C terminal of clostridium perfringens enterotoxin as a vaccine candidate.

Bioengineered 2018 01 30;9(1):170-177. Epub 2017 Nov 30.

a Department of Pathobiology , School of Veterinary Medicine, Shiraz University , Shiraz , Iran.

This study aimed to design a novel chimeric protein in silico to serve as a serotype-independent vaccine candidate against Shigella. The chimera contains amino acid residues 240-460 of Shigella invasion plasmid antigen B (IpaB) and the C-terminus of Clostridium perfringens enterotoxin (C-CPE). Amino acid sequences of 537 peptide linkers were obtained from two protein linker databases. Read More

View Article

Download full-text PDF

Source
https://www.tandfonline.com/doi/full/10.1080/21655979.2017.1
Publisher Site
http://dx.doi.org/10.1080/21655979.2017.1373535DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5972921PMC
January 2018
8 Reads

Bio-invasive ascidians in Ireland: A threat for the shellfish industry but also a source of high added value products.

Bioengineered 2018 01 1;9(1):55-60. Epub 2017 Dec 1.

a Department of Zoology , School of Natural Science, Ryan Institute, National University of Ireland , Galway , Ireland.

In October 2016, a rapid assessment survey of ascidian species was conducted in shellfish farms at Killary Fjord, in the west of Ireland. Two non-indigenous solitary ascidians Ascidiella aspersa and Corella eumoyta were recorded for the first time in shellfish farms at this location. Both invasive ascidians have the potential to greatly reduce mussel production in Killary Fjord by competing with shellfish for food and habitat. Read More

View Article

Download full-text PDF

Source
https://www.tandfonline.com/doi/full/10.1080/21655979.2017.1
Publisher Site
http://dx.doi.org/10.1080/21655979.2017.1392421DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5972928PMC
January 2018
7 Reads

An In vivo study: Adjuvant activity of poly-n-vinyl-2-pyrrolidone-co-acrylic acid on immune responses against Melanoma synthetic peptide.

Bioengineered 2018 Jan 28;9(1):134-143. Epub 2017 Sep 28.

c Department of Bioengineering , Yıldız Technical University, Davutpaşa Caddesi , Esenler, İstanbul , Turkey.

Peptides have been studied as an important class of components in medicine to control many major diseases with vaccination. Polymers as adjuvants are capable of enhancing the vaccine potential against various diseases by improving the delivery of antigens, and they reduce the booster doses of vaccines. In brief, polymers are promising candidates for peptide-based vaccine delivery platforms. Read More

View Article

Download full-text PDF

Source
https://www.tandfonline.com/doi/full/10.1080/21655979.2017.1
Publisher Site
http://dx.doi.org/10.1080/21655979.2017.1373529DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5972930PMC
January 2018
11 Reads

Preparation of umami octopeptide with recombined Escherichia coli: Feasibility and challenges.

Bioengineered 2018 01 28;9(1):166-169. Epub 2017 Sep 28.

a Key Laboratory of Meat Processing of Sichuan , Chengdu University , Chengdu , China.

The taste of umami peptide H-Lys-Gly-Asp-Glu-Glu-Ser-Leu-Ala-OH (LGAGGSLA) is controversial. One possible reason for this controversy is the use of chemically synthesized LGAGGSLA to confirm its taste. To explore other ways to further confirm the flavor of LGAGGSLA, we developed a new strategy to prepare a bio-source peptide by adopting a gene engineering method to express LGAGGSLA in recombinant Escherichia coli. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/21655979.2017.1378839DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5972915PMC
January 2018
28 Reads

Green biologics: The algal chloroplast as a platform for making biopharmaceuticals.

Bioengineered 2018 01 29;9(1):48-54. Epub 2017 Sep 29.

b Algal Research Group, Institute of Structural and Molecular Biology , University College London , Gower Street , London , United Kingdom.

Most commercial production of recombinant pharmaceutical proteins involves the use of mammalian cell lines, E. coli or yeast as the expression host. However, recent work has demonstrated the potential of eukaryotic microalgae as platforms for light-driven synthesis of such proteins. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/21655979.2017.1377867DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5972929PMC
January 2018
14 Reads

Optimization of saccharification potential of recombinant xylanase from Bacillus licheniformis.

Bioengineered 2018 01 28;9(1):159-165. Epub 2017 Sep 28.

e Institute of Biochemistry & Biotechnology , University of Veterinary and Animal Sciences , Lahore , Pakistan.

Saccharification potential of xylanase enzyme cloned from Bacillus licheniformis into E. coli BL21 (DE3) was evaluated against plant biomass for the production of bioethanol. The expression of cloned gene was studied and conditions were optimized for its large scale production. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/21655979.2017.1373918DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5972937PMC
January 2018
3 Reads

The effect of three novel feature extraction methods on the prediction of the subcellular localization of multi-site virus proteins.

Bioengineered 2018 01 22;9(1):196-202. Epub 2017 Nov 22.

a School of Information Science and Engineering, University of Jinan , Jinan , China.

Experimental methods play a crucial role in identifying the subcellular localization of proteins and building high-quality databases. However, more efficient, automated computational methods are required to predict the subcellular localization of proteins on a large scale. Various efficient feature extraction methods have been proposed to predict subcellular localization, but challenges remain. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/21655979.2017.1373536DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5972939PMC
January 2018
1 Read

Proteomic analysis reveals that a global response is induced by subinhibitory concentrations of ampicillin.

Bioengineered 2017 Nov 21;8(6):732-741. Epub 2017 Sep 21.

a The First Affiliated Hospital of Guangzhou Medical University , Guangzhou Institute of Respiratory Disease, State Key Laboratory of Respiratory Disease , Guangzhou , China.

In this study, a recipient-donor co-culture system was used to research the effect of subinhibitory concentrations of antibiotics on horizontal transmission in bacteria and the influence of antibiotics on protein expression. We employed two-dimensional gel electrophoresis combined with mass spectrometry to compare the protein expression profiles in systems with or without 0.5 × the minimum inhibitory concentration of ampicillin. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/21655979.2017.1373532DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5736336PMC
November 2017
25 Reads

Survey of gene splicing algorithms based on reads.

Bioengineered 2017 Nov 21;8(6):750-758. Epub 2017 Sep 21.

a Department of Computer Science & Technology , Heilongjiang University , Harbin , China.

Gene splicing is the process of assembling a large number of unordered short sequence fragments to the original genome sequence as accurately as possible. Several popular splicing algorithms based on reads are reviewed in this article, including reference genome algorithms and de novo splicing algorithms (Greedy-extension, Overlap-Layout-Consensus graph, De Bruijn graph). We also discuss a new splicing method based on the MapReduce strategy and Hadoop. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/21655979.2017.1373538DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5736332PMC
November 2017
4 Reads

Bioengineering of crop plants for improved tetrahydrofolate production.

Bioengineered 2018 Jan 21;9(1):152-158. Epub 2017 Sep 21.

a School of Biotechnology , Gautam Buddha University , Greater Noida , U.P. India.

De novo synthesis of folates in plants is tightly regulated through feedback-regulation of certain pathway catalysts. Recently, we investigated the prospects of incessant production of folates in an evolutionary conjunction, through the overexpression of feedback targeted and evolutionarily conserved heterologous E.coli dihydroneopterin aldolase (EcDHNA) in tobacco. Read More

View Article

Download full-text PDF

Source
https://www.tandfonline.com/doi/full/10.1080/21655979.2017.1
Publisher Site
http://dx.doi.org/10.1080/21655979.2017.1373537DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5972932PMC
January 2018
7 Reads

A glutathione peroxidase from Antarctic psychrotrophic bacterium Pseudoalteromonas sp. ANT506: Cloning and heterologous expression of the gene and characterization of recombinant enzyme.

Bioengineered 2017 Nov 21;8(6):742-749. Epub 2017 Sep 21.

a School of Marine and Technology , Harbin Institute of Technology , Weihai , Shandong , P.R. China.

A glutathione peroxidase (GPx) gene, designated as PsGPx, was cloned from Antarctic psychrotrophic bacterium Pseudoalteromonas sp. ANT506 and expressed in Escherichia coli. The full-length PsGPx contained a 585-bp encoding 194 amino acids with predicted molecular masses of approx. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/21655979.2017.1373534DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5736345PMC
November 2017
23 Reads

Recombinant scorpion toxins: Focus on four-disulfide peptide blockers of Kv1-channels.

Bioengineered 2018 01 21;9(1):25-29. Epub 2017 Sep 21.

a Bioengineering Department, Biological Faculty , Lomonosov Moscow State University , Moscow , Russia.

We have recently developed a simple and effective bioengineering approach to large-scale production of alpha-KTx, peptide toxins from scorpion venoms, that block voltage-gated potassium channels with high affinity and specificity. This approach was successfully approved for different peptides containing three disulfide bonds. To extend this method to production of peptide toxins with four disulfide bridges, in particular, maurotoxin and hetlaxin, appropriate conditions of a cleavage reaction with tobacco etch virus (TEV) protease need to be found. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/21655979.2017.1373530DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5972927PMC
January 2018
12 Reads

Specificity of peptidases secreted by filamentous fungi.

Bioengineered 2018 01 21;9(1):30-37. Epub 2017 Sep 21.

a School of Pharmaceutical Sciences of Ribeirão Preto , University of São Paulo , Ribeirão Preto , Brazil.

Peptidases are enzymes that cleave peptide bonds, yielding proteins and peptides. Enzymes in this class also perform several other functions, regulating the activation or inactivation of target substrates via proteolysis. Owing to these functions, peptidases have been extensively used in industrial and biotechnological applications. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/21655979.2017.1373531DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5972931PMC
January 2018
3 Reads

Corrigendum.

Authors:

Bioengineered 2017 11 18;8(6):759. Epub 2017 Aug 18.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/21655979.2017.1361259DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5736342PMC
November 2017

Relationship between PPARα mRNA expression and mitochondrial respiratory function and ultrastructure of the skeletal muscle of patients with COPD.

Bioengineered 2017 Nov 11;8(6):723-731. Epub 2017 Aug 11.

a Department of Respiratory Critical Care Medicine , the First Affiliated Hospital of Kunming Medical University , Kunming , China.

Peripheral muscle dysfunction is an important complication in patients with chronic obstructive pulmonary disease (COPD). The objective of this study was to explore the relationship between the levels of peroxisome proliferator-activated receptor α (PPARα) mRNA expression and the respiratory function and ultrastructure of mitochondria in the vastus lateralis of patients with COPD. Vastus lateralis biopsies were performed on 14 patients with COPD and 6 control subjects with normal lung function. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/21655979.2017.1346757DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5736346PMC
November 2017
2 Reads

Regulatory roles of 24-epibrassinolide in tolerance of Acacia gerrardii Benth to salt stress.

Bioengineered 2018 Jan 1;9(1):61-71. Epub 2017 Nov 1.

e Institute of Landscape Biogeochemistry, Leibniz Centre for Agricultural Landscape Research (ZALF) , Müncheberg , Germany.

This experiment aimed to investigate the role of 24-epibrassinolide (EBL) against NaClinduced salinity stress in Acacia gerrardii Benth. NaCl (200 mM) imparted deleterious effects on the growth and chlorophyll contents of A. gerrardii, but foliar application of EBL (1. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/21655979.2017.1297348DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5972912PMC
January 2018
3 Reads

Surrogate strains of human pathogens for field release.

Bioengineered 2018 01 26;9(1):17-24. Epub 2017 Jul 26.

a Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Plus Program), Center for Systems and Synthetic Biotechnology , Institute for the BioCentury, Korea Advanced Institute of Science and Technology (KAIST) , Daejeon , Republic of Korea.

Surrogate microorganisms, in short surrogates, are an essential part of pathogen research. Compared to surrogates used in controlled laboratory environments, surrogates for field release are restricted by concerns about human and environmental safety. For field research of food-borne pathogens, strains of an attenuated pathogen or strains of genetically close non-pathogenic species have been used as surrogates. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/21655979.2017.1349044DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5972925PMC
January 2018
1 Read

Polyvinylsulfonic acid: A Low-cost RNase inhibitor for enhanced RNA preservation and cell-free protein translation.

Bioengineered 2018 Jan 29;9(1):90-97. Epub 2017 Jun 29.

a Department of Chemical Engineering , Brigham Young University , Provo , UT , USA.

The effectiveness and economics of polyvinyl sulfonic acid (PVSA) as a ribonuclease inhibitor for in vitro systems is reported. PVSA was shown to inhibit RNA cleavage in the presence of RNase A as well as in the presence of Escherichia coli lysate, suggesting that PVSA can act as a broader ribonuclease inhibitor. In addition, PVSA was shown to improve the integrity of mRNA transcripts by up to 5-fold in vitro as measured by their translational viability. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/21655979.2017.1313648DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5972934PMC
January 2018
8 Reads

Genome mining, in silico validation and phase selection of a novel aldo-keto reductase from Candida glabrata for biotransformation.

Bioengineered 2018 01 13;9(1):186-195. Epub 2017 Jul 13.

c International College of Engineering and Management (University of Central Lancashire, UK Affiliation) , Muscat , Oman.

Previously, we published cloning, overexpression, characterization and subsequent exploitation of a carbonyl reductase (cr) gene, belonging to general family aldo-keto reductase from Candida glabrata CBS138 to convert keto ester (COBE) to a chiral alcohol (ethyl-4-chloro-3-hydroxybutanoate or CHBE). Exploiting global transcription factor CRP, rDNA and transporter engineering, we have improved batch production of CHBE by trinomial bioengineering. Herein, we present the exploration of cr gene in Candida glabrata CBS138 through genome mining approach, in silico validation of its activity and selection of its biocatalytic phase. Read More

View Article

Download full-text PDF

Source
https://www.tandfonline.com/doi/full/10.1080/21655979.2017.1
Publisher Site
http://dx.doi.org/10.1080/21655979.2017.1342911DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5972913PMC
January 2018
5 Reads

Uncovering the repertoire of fungal secondary metabolites: From Fleming's laboratory to the International Space Station.

Authors:
Tomasz Boruta

Bioengineered 2018 01 12;9(1):12-16. Epub 2017 Jul 12.

a Lodz University of Technology , Faculty of Process and Environmental Engineering, Department of Bioprocess Engineering , Lodz , Poland.

Fungi produce a variety of secondary metabolites (SMs), low-molecular weight compounds associated with many potentially useful biologic activities. The examples of biotechnologically relevant fungal metabolites include penicillin, a β-lactam antibiotic, and lovastatin, a cholesterol-lowering drug. The discovery of pharmaceutical lead compounds within the microbial metabolic pools relies on the selection and biochemical characterization of promising strains. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/21655979.2017.1341022DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5972916PMC
January 2018
9 Reads

Simple fluorescence-based detection of protein kinase A activity using a molecular beacon probe.

Bioengineered 2017 Nov 11;8(6):716-722. Epub 2017 Aug 11.

a State Key Laboratory of Medical Genetics & School of Life Sciences , Central South University , Changsha , China.

Protein kinase A was detected by quantifying the amount of ATP used after a protein kinase reaction. The ATP assay was performed using the T4 DNA ligase and a molecular beacon (MB). In the presence of ATP, DNA ligase catalyzed the ligation of short DNA. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/21655979.2017.1338219DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5736335PMC
November 2017
23 Reads

Development of CRISPR/Cas9 mediated virus resistance in agriculturally important crops.

Bioengineered 2017 May;8(3):274-279

b Amity Institute of Microbial Technology, Amity University , Noida , India.

Clustered regulatory interspaced short palindromic repeats (CRISPR)/CRISPR associated nuclease 9 (Cas9) system of targeted genome editing has already revolutionized the plant science research. This is a RNA guided programmable endonuclease based system composed of 2 components, the Cas9 nuclease and an engineered guide RNA targeting any DNA sequence of the form N20-NGG for novel genome editing applications. The CRISPR/Cas9 technology of targeted genome editing has been recently applied for imparting virus resistance in plants. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/21655979.2017.1297347DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5470520PMC
May 2017
21 Reads

Microbial production of mevalonate by recombinant Escherichia coli using acetic acid as a carbon source.

Bioengineered 2018 Jan 2;9(1):116-123. Epub 2017 Jun 2.

a CAS Key Laboratory of Biobased Materials , Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences , Qingdao , China.

We sought to produce mevalonate, an important organic acid, by recombinant Escherichia coli, using acetic acid, a less costly alternative feedstock, as a carbon source. In this study, the mevalonate biosynthesis pathway originating with acetate was constructed in recombinant E. coli, resulting in the production of 1. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/21655979.2017.1323592DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5972924PMC
January 2018
67 Reads

Mining of a phospholipase D and its application in enzymatic preparation of phosphatidylserine.

Bioengineered 2018 Jan 16;9(1):80-89. Epub 2017 May 16.

a School of Pharmaceutical Science , Jiangnan University , Wuxi , PR China.

Phosphatidylserine (PS) is useful as the additive in industries for memory improvement, mood enhancement and drug delivery. Conventionally, PS was extracted from soybeans, vegetable oils, egg yolk, and biomass; however, their low availability and high extraction cost were limiting factors. Phospholipase D (PLD) is a promising tool for enzymatic synthesis of PS due to its transphosphatidylation activity. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/21655979.2017.1308992DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5972935PMC
January 2018
28 Reads

FabG can function as PhaB for poly-3-hydroxybutyrate biosynthesis in photosynthetic cyanobacteria Synechocystis sp. PCC 6803.

Bioengineered 2017 Nov 19;8(6):707-715. Epub 2017 May 19.

b Marine Bioengineering Group, Dalian Institute of Chemical Physics , Chinese Academy of Sciences , Dalian , China.

The production of poly-3-hydroxybutyrate (PHB) by photosynthetic cyanobacteria is a potentially sustainable production method for the biodegradable plastics industry. β-Ketoacyl-ACP reductase (FabG), from the photosynthetic cyanobacterium Synechocystis sp. PCC 6803 (SpFabG), is the first NADPH-dependent reductase in the fatty acid biosynthesis pathway. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/21655979.2017.1317574DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5736341PMC
November 2017
6 Reads

Enhanced microalgal lipid production with media engineering of potassium nitrate as a nitrogen source.

Bioengineered 2018 Jan 4;9(1):98-107. Epub 2017 May 4.

a Department of Biotechnology and Bioinformatics , Jaypee University of Information Technology , Waknaghat , HP , India.

Algal biofuels are far from a commercial reality due to the technical challenges associated with their growth and lipid extraction procedures. In this study, we investigated the effect of 4 different media and 5 different nitrogen sources at 5 levels on the growth, biomass and lipid productivity of Scenedesmus sp and Chlorella sp The hypothesis was that a nitrogen source can be identified that provides enough stress to accumulate lipids without compromising significantly on biomass and lipid productivity. A maximum specific growth rate and doubling per day have been observed with algal species using modified BG-11 medium. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/21655979.2017.1316440DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5972933PMC
January 2018
3 Reads

A synthetic hybrid promoter for D-xylonate production at low pH in the tolerant yeast Candida glycerinogenes.

Bioengineered 2017 Nov 4;8(6):700-706. Epub 2017 May 4.

a The Key Laboratory of Carbohydrate Chemistry and Biotechnology , Ministry of Education, School of Biotechnology, Jiangnan University , Wuxi , China.

The tolerant yeast Candida glycerinogenes, with high D-xylonate and low-pH tolerances, was used as the host for D-xylonate production at low pH in this study. A low-pH inducible promoter, pGUKd, was engineered using the core promoter of the glyceraldehyde-3-phosphate dehydrogenase gene (pGAP) combined with the upstream activating sequence of the promoter of the guanylate kinase gene (pGUK1) that had substituted pH-responsive TF binding sites. The recombinant cells that expressed GFP from the hybrid promoter pGUKd displayed dramatically increased fluorescence intensity at pH 2. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/21655979.2017.1312229DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5736338PMC
November 2017
1 Read

Statistical optimization for the production of recombinant cold-adapted superoxide dismutase in E. coli using response surface methodology.

Bioengineered 2017 Nov 4;8(6):693-699. Epub 2017 May 4.

a School of Marine Science and Technology , Harbin Institute of Technology , Weihai , P.R. China.

Cold-adapted superoxide dismutase (SOD) with higher catalytic activity at lower temperature has great amount of applications in many aspects as an industrial enzyme. The application of recombinant enzyme in gene engineering and microbial fermentation technology is an effective way to obtain high-yield product. In this study, to obtain the recombinant SOD in E. Read More

View Article

Download full-text PDF

Source
https://www.tandfonline.com/doi/full/10.1080/21655979.2017.1
Publisher Site
http://dx.doi.org/10.1080/21655979.2017.1303589DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5736329PMC
November 2017
4 Reads

Bioengineering toward direct production of immobilized enzymes: A paradigm shift in biocatalyst design.

Bioengineered 2018 Jan 19;9(1):6-11. Epub 2017 May 19.

b Institute of Fundamental Sciences, Massey University , Palmerston North , New Zealand.

The need for cost-effectively produced and improved biocatalysts for industrial, pharmaceutical and environmental processes is steadily increasing. While enzyme properties themselves can be improved via protein engineering, immobilization by attachment to carrier materials remains a critical step for stabilization and process implementation. A new emerging immobilization approach, the in situ immobilization, enables simultaneous production of highly active enzymes and carrier materials using bioengineering/synthetic biology of microbial cells. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/21655979.2017.1325040DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5972917PMC
January 2018
1 Read

Anti-microbial coating innovations to prevent infectious diseases (AMiCI): Cost action ca15114.

Bioengineered 2017 Nov 19;8(6):679-685. Epub 2017 May 19.

n Zuyd University of Applied Sciences , Heerlen , The Netherlands.

Worldwide, millions of patients are affected annually by healthcare-associated infection (HCAI), impacting up to 80,000 patients in European Hospitals on any given day. This represents not only public health risk, but also an economic burden. Complementing routine hand hygiene practices, cleaning and disinfection, antimicrobial coatings hold promise based, in essence, on the application of materials and chemicals with persistent bactericidal or -static properties onto surfaces or in textiles used in healthcare environments. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/21655979.2017.1323593DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5736330PMC
November 2017
7 Reads