844 results match your criteria Bioconjugate Chemistry [Journal]


Multifunctional hybrid 3D network based on hyaluronic acid and a copolymer containing pendant spiroacetal moieties.

Int J Biol Macromol 2018 Dec 5. Epub 2018 Dec 5.

"Gr. T. Popa" University of Medicine and Pharmacy, Iasi, Romania.

The elasticity, soft consistency and similarity to the human body are important characteristics of the gels. Such similarities allow the hydrogels to be biocompatible and, as a consequence, they are very attractive to be applied as biomaterials. This study presents a new strategy for developing a biohybrid complex based on a natural/synthetic polymer conjugate as gel type structure enriched with quercetin, which can be a relevant biomaterial for its antimicrobial properties. Read More

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December 2018

Aldehydes can switch the chemoselectivity of electrophiles in protein labeling.

Org Biomol Chem 2018 Dec;16(48):9377-9381

Organic and Bioconjugate Chemistry Laboratory, Department of Chemistry, Indian Institute of Science Education and Research Bhopal, Bhauri, Bhopal Bypass Road, Bhopal 462066, India.

We show that the chemoselectivity of an electrophile in protein labeling can be promiscuous. An aldehyde enables switching of chemoselectivity of an epoxide and a sulfonate ester along with an enhanced rate of reaction. The chemical technology renders single-site installation of diverse probes on a protein and delivers analytically pure tagged proteins. Read More

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December 2018

How nanomedicine can be used in the treatment of tumors: an interview with Dr Gang Zheng.

Nanomedicine (Lond) 2018 Nov 13. Epub 2018 Nov 13.

Princess Margaret Cancer Centre, Toronto, M5G 1L7, Canada.

Dr Zheng received his PhD in 1999 from SUNY Buffalo in Medicinal Chemistry. Following 2 years of postdoctoral training in photodynamic therapy at the Roswell Park Cancer Institute, he joined the University of Pennsylvania in 2001 as an Assistant Professor of Radiology, where he established the molecular imaging chemistry program and introduced photodynamic molecular beacons and lipoprotein-like nanoparticles. Since moving to Canada in 2006, his research has been focused on developing clinically translatable technology platforms to combat cancer. Read More

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November 2018
2 Reads

NOTA and NODAGA [Tc]Tc- and [Re]Re-Tricarbonyl Complexes: Radiochemistry and First Example of a [Tc]Tc-NODAGA Somatostatin Receptor-Targeting Bioconjugate.

Bioconjug Chem 2018 Nov 26. Epub 2018 Nov 26.

Research Service , Harry S. Truman Memorial Veterans' Hospital , Columbia , Missouri 65201 , United States.

With the long-term goal of developing theranostic agents for applications in nuclear medicine, in this work we evaluated the well-known NOTA and NODAGA chelators as bifunctional chelators (BFCs) for the [Tc/Re]Tc/Re-tricarbonyl core. In particular, we report model complexes of the general formula fac-[M(L)(CO)] (M = Re, Tc, Re) where L denotes NOTA-Pyr (1) or NODAGA-Pyr (2), which are derived from conjugation of NOTA/NODAGA with pyrrolidine (Pyr). Further, as proof-of-principle, we synthesized the peptide bioconjugate NODAGA-sst-ANT (3) and explored its complexation with the fac-[Re(CO)] and fac-[Tc][Tc(CO)] cores; sst-ANT denotes the somatostatin receptor (SSTR) antagonist 4-NO-Phe-c(DCys-Tyr-DTrp-Lys-Thr-Cys)-DTyr-NH. Read More

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November 2018
14 Reads

Array-based "Chemical Nose" Sensing in Diagnostics and Drug Discovery.

Angew Chem Int Ed Engl 2018 Oct 22. Epub 2018 Oct 22.

University of Massachusetts, Department of Chemistry, 710 North Pleasant Street, 1003, Amherst, UNITED STATES.

Array-based sensor 'chemical nose/tongue' platforms are inspired by the mammalian olfactory system. Multiple sensor elements in these devices selectively interact with target analytes, producing a distinct pattern of response and enabling analyte identification. This approach offers unique opportunities relative to 'traditional' highly specific sensor elements such as antibodies. Read More

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October 2018
6 Reads

Site-Specific Antibody Drug Conjugates Using Streamlined Expressed Protein Ligation.

Bioconjug Chem 2018 Nov 22;29(11):3503-3508. Epub 2018 Oct 22.

ProteoDesign , C/Baldiri Reixach 10 , Barcelona 08028 , Spain.

Antibody-Drug Conjugates (ADCs) have been shown to produce clinical benefit in cancer patient thanks to their ability to target highly cytotoxic small molecules to tumor cells. However, the development of these complex molecules faces significant challenges due to the need to combine a large biologic drug with a small molecule drug to generate the desired bioconjugate. We describe here the use of a protein ligation methodology, based on the native chemical ligation reaction to generate site-specific Antibody-Drug Conjugates, which does not require the incorporation of unnatural modifications into the antibody. Read More

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November 2018
4 Reads

Synthesis of monofunctional platinum(iv) carboxylate precursors for use in Pt(iv)-peptide bioconjugates.

Dalton Trans 2018 Nov 18;47(43):15465-15476. Epub 2018 Oct 18.

Inorganic Chemistry I - Bioinorganic Chemistry, Faculty of Chemistry and Biochemistry, Ruhr-University Bochum, Universitätsstraße 150, 44801 Bochum, Germany.

Herein we present platinum(iv) bioconjugates with polyarginine peptides as prospective prodrug delivery systems. Asymmetrical platinum(iv) complexes 3 were obtained via oxidation of parent platinum(ii) complexes 2 with N-bromosuccinimide (NBS) in the presence of succinic anhydride. The combination of these two oxidation reagents furnishes the platinum(iv) environment with two different axial ligands, one of which bears a free carboxylic acid. Read More

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November 2018
3 Reads

Nanofilamentous Virus-Based Dynamic Hydrogels with Tunable Internal Structures, Injectability, Self-Healing, and Sugar Responsiveness at Physiological pH.

Langmuir 2018 Oct 17;34(43):12914-12923. Epub 2018 Oct 17.

Key Laboratory of Functional Polymer Materials of Ministry of Education, Institute of Polymer Chemistry, College of Chemistry , Nankai University , Tianjin 300071 , China.

With expanding applications of hydrogels in diverse fields ranging from biomaterials to sensors, actuators, and soft robotics, there is an urgent need to endow one single gel with multiple physicochemical properties, such as stimuli-responsiveness, injectability, self-healing, and tunable internal structures. However, it is challenging to simultaneously incorporate these highly sought-after properties into one single gel. Herein, a conceptual hydrogel system with all of these properties is presented via combining bioconjugate chemistry, filamentous viruses, and dynamic covalent bonds. Read More

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October 2018
1 Read

Synthesis and Characterization of Placental Chondroitin Sulfate A (plCSA)-Targeting Lipid-Polymer Nanoparticles.

J Vis Exp 2018 Sep 18(139). Epub 2018 Sep 18.

Laboratory for Reproductive Health, Institute of Biomedicine and Biotechnology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences (CAS);

An effective cancer therapeutic method reduces and eliminates tumors with minimal systemic toxicity. Actively targeting nanoparticles offer a promising approach to cancer therapy. The glycosaminoglycan placental chondroitin sulfate A (plCSA) is expressed on a wide range of cancer cells and placental trophoblasts, and malarial protein VAR2CSA can specifically bind to plCSA. Read More

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September 2018
1 Read

Ru(ii)-Peptide bioconjugates with the cppH linker (cppH = 2-(2'-pyridyl)pyrimidine-4-carboxylic acid): synthesis, structural characterization, and different stereochemical features between organic and aqueous solvents.

Dalton Trans 2018 Oct 4. Epub 2018 Oct 4.

Inorganic Chemistry I - Bioinorganic Chemistry, Ruhr University Bochum, Faculty of Chemistry and Biochemistry, Universitätsstraße 150, 44801 Bochum, Germany.

Three new Ru(ii) bioconjugates with the C-terminal hexapeptide sequence of neurotensin, RRPYIL, namely trans,cis-RuCl2(CO)2(cppH-RRPYIL-κNp) (7), [Ru([9]aneS3)(cppH-RRPYIL-κNp)(PTA)](Cl)2 (8), and [Ru([9]aneS3)Cl(cppH-RRPYIL-κNp)]Cl (11), where cppH is the asymmetric linker 2-(2'-pyridyl)pyrimidine-4-carboxylic acid, were prepared in pure form and structurally characterized in solution. The cppH linker is capable of forming stereoisomers (i.e. Read More

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October 2018
1 Read

A photoelectrochemical sensor for highly sensitive detection of amyloid beta based on sensitization of Mn:CdSe to BiWO/CdS.

Biosens Bioelectron 2018 Dec 10;122:37-42. Epub 2018 Sep 10.

Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China; State Key Laboratory of Analytical Chemistry for Life Science, Department of Chemistry, Nanjing University, Nanjing 210023, PR China.

A high sensitivity photoelectrochemical (PEC) immunosensor for amyloid beta (Aβ) detection, which has great neurotoxic effect on the progression of Alzheimer's disease (AD), was presented based on the sensitization of Mn doped CdSe (Mn:CdSe) to BiWO/CdS electrode. BiWO was synthesized successfully with a unique flower-like amorphous structure, providing a merit to load functional CdS for obtaining an expected PEC response. Mn:CdSe was used to label Aβ for acquiring Mn:CdSe-Aβ bioconjugate and enhancing the detection sensitivity via the competitive immunoreaction of Mn:CdSe-Aβ and Aβ with antibody immobilized on BiWO/CdS electrode. Read More

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December 2018
8 Reads

Probing the interaction of a quercetin bioconjugate with Bcl-2 in living human cancer cells with in-cell NMR spectroscopy.

FEBS Lett 2018 Oct 29;592(20):3367-3379. Epub 2018 Sep 29.

Section of Organic Chemistry and Biochemistry, Department of Chemistry, University of Ioannina, Greece.

In-cell NMR spectroscopy has emerged as a powerful technique for monitoring biomolecular interactions at an atomic level inside intact cells. However, current methodologies are inadequate at charting intracellular interactions of nonlabeled proteins and require their prior isotopic labeling. Herein, we describe for the first time the monitoring of the quercetin-alanine bioconjugate interaction with the nonlabeled antiapoptotic protein Bcl-2 inside living human cancer cells. Read More

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October 2018
3.170 Impact Factor

Antibody-Drug Conjugates with Pyrrole-Based KSP Inhibitors as the Payload Class.

Angew Chem Int Ed Engl 2018 Nov 15;57(46):15243-15247. Epub 2018 Oct 15.

Bayer AG R&D Pharmaceuticals, Aprather Weg 18a, 42113, Wuppertal, Germany.

The number of cytotoxic payload classes successfully employed in antibody-drug conjugates (ADCs) is still rather limited. The identification of ADC payloads with a novel mode of action will increase therapeutic options and potentially increase the therapeutic window. Herein, we describe the utilization of kinesin spindle protein inhibitors (KSPi) as a novel payload class providing highly potent ADCs against different targets, for instance HER-2 or TWEAKR/Fn14. Read More

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November 2018
14 Reads

Bioconjugation with Maleimides: A Useful Tool for Chemical Biology.

Chemistry 2018 Aug 10. Epub 2018 Aug 10.

Bioorganic Chemistry Department, Research Institute for Medicines, (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, Av. Prof. Gama Pinto, 1649-003, Lisboa, Portugal.

Maleimide chemistry stands out in the bioconjugation toolbox by virtue of its synthetic accessibility, excellent reactivity, and practicability. The second-generation of clinically approved antibody-drug conjugates (ADC) and much of the current ADC pipeline in clinical trials contain the maleimide linkage. However, thiosuccinimide linkages are now known to be less robust than once thought, and ergo, are correlated with suboptimal pharmacodynamics, pharmacokinetics, and safety profiles in some ADC constructs. Read More

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August 2018
9 Reads

Vaccine Development for Urinary Tract Infections: Where Do We Stand?

Eur Urol Focus 2018 Aug 6. Epub 2018 Aug 6.

Department of Urology, Ludwig-Maximilians-University, Munich, Germany.

Urinary tract infections (UTIs) are among the most common bacterial infections. Its management has become increasingly challenging due to antimicrobial resistance. The four mainstays to tackle this crisis rely on the development of new antibiotic agents, the introduction of preventive and alternative antimicrobial strategies, the concept of antimicrobial stewardship, and effective hygiene measures. Read More

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August 2018
1 Read

Immunochemical rapid determination of quinoxyfen, a priority hazardous pollutant.

Chemosphere 2018 Nov 30;211:302-307. Epub 2018 Jul 30.

Institute of Agrochemistry and Food Technology (IATA), Spanish Council for Scientific Research (CSIC), Agustí Escardino 7, 46980, Paterna, Valencia, Spain. Electronic address:

In 2013, quinoxyfen was included in the list of priority hazard pollutants of the European Water Framework Directive due to its toxicity to aquatic organisms. However, few analytical methods for the analysis of this fungicide have been reported and no rapid immunochemical methods have been published so far. In the present study, immunoreagents for quinoxyfen analysis were generated for the first time and an enzyme-linked immunosorbent assay was developed. Read More

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November 2018
1 Read

'Reverse' Hofmeister effects on the sol-gel transition rates for an α-helical peptide-PEG bioconjugate.

Phys Chem Chem Phys 2018 Aug;20(30):20287-20295

Department of Chemical Engineering, The City College of City University of New York, 140th Street and Convent Avenue, Steinman Hall T313, New York, New York 10031, USA.

We examine the dynamics of the sol-gel transition for end-functionalized linear- and 4-arm-peptides bioconjugated to poly-ethylene glycol (PEG) in aqueous environments with increasingly chaotropic (Cl- < Br- < I-) anions. A 23-amino acid peptide sequence is rationally designed to self-assemble upon folding into the ordered α-helical conformation due to the hydrophobic effect. We use Attenuated Total Reflection-Fourier Transform Infrared Spectroscopy (ATR-FTIR) to quantify the ensemble average reversible secondary structure transitions as a function of electrolyte concentration and specific ion effects along the Hofmeister series. Read More

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High-Throughput Signal-On Photoelectrochemical Immunoassay of Lysozyme Based on Hole-Trapping Triggered by Disintegrating Bioconjugates of Dopamine-Grafted Silica Nanospheres.

ACS Sens 2018 Aug 23;3(8):1480-1488. Epub 2018 Jul 23.

Key Laboratory of Pesticide & Chemical Biology of Ministry of Education, College of Chemistry , Central China Normal University , Wuhan 430079 , P. R. China.

A unique split-type photoelectrochemical (PEC) immunoassay has been constructed for detection of low-abundance biocompounds (lysozyme, Lyz, used in this case) via a new trigger strategy by disintegrating bioconjugates of dopamine-grafted silica nanospheres (DA@SiONSs) for signal amplification. The preferred electron donor assembly of DA@SiONSs is first used as a molecular printboard for positioning anti-Lyz secondary antibody (Ab) through an amide reaction. With specific immunoreactions in a high-binding microplate, a sandwich immunoassay, the DA@SiONSs-based bioconjugate is achieved. Read More

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August 2018
9 Reads

Magnetic force assisted electrochemical sensor for the detection of thrombin with aptamer-antibody sandwich formation.

Biosens Bioelectron 2018 Oct 5;117:480-486. Epub 2018 Jul 5.

Department of Chemistry, Pusan National University, 2 Busandaehak-ro 63beon-gil, Geumjeong-gu, Busan 46241, Republic of Korea. Electronic address:

A magnetic force assisted electrochemical aptamer-antibody sandwich assay (MESA) was developed for the detection of thrombin as a model protein in serum samples. The MESA using the formation of sandwich complexes on the electrochemical sensor probe for reaction and the removal of unbound bioconjugates from the sensor surface without washing are controlled by a magnetic field. Thrombin was determined by the cathodic currents of a toluidine blue O (TBO) attached with thrombin antibody modified magnetic nanoparticle (MNP) at the sensor surface. Read More

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October 2018
25 Reads

DNA Oligonucleotide-Functionalized Liposomes: Bioconjugate Chemistry, Biointerfaces, and Applications.

Langmuir 2018 Jul 9. Epub 2018 Jul 9.

Department of Chemistry, Waterloo Institute for Nanotechnology , University of Waterloo , Waterloo , Ontario N2L 3G1 , Canada.

Interfacing DNA with liposomes has produced a diverse range of programmable soft materials, devices, and drug delivery vehicles. By simply controlling liposomal composition, bilayer fluidity, lipid domain formation, and surface charge can be systematically varied. Recent development in DNA research has produced not only sophisticated nanostructures but also new functions including ligand binding and catalysis. Read More

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Site-Specific Dual Labeling of Proteins on Cysteine Residues with Chlorotetrazines.

Angew Chem Int Ed Engl 2018 Aug 5;57(33):10646-10650. Epub 2018 Jul 5.

Institut de Chimie Moléculaire de l'Université de Bourgogne, UMR6302, CNRS, Université Bourgogne Franche-Comté, 9 avenue Alain Savary, 21000, Dijon, France.

Dual-labeled biomolecules constitute a new generation of bioconjugates with promising applications in therapy and diagnosis. Unfortunately, the development of these new families of biologics is hampered by the technical difficulties associated with their construction. In particular, the site specificity of the conjugation is critical as the number and position of payloads can have a dramatic impact on the pharmacokinetics of the bioconjugate. Read More

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August 2018
2 Reads

Brain Delivery of Multifunctional Dendrimer Protein Bioconjugates.

Adv Sci (Weinh) 2018 May 23;5(5):1700897. Epub 2018 Feb 23.

Institute of Physiology University Medical Center of Johannes Gutenberg University Mainz Duesbergweg 6 D-55128 Mainz Germany.

Neurological disorders are undoubtedly among the most alarming diseases humans might face. In treatment of neurological disorders, the blood-brain barrier (BBB) is a challenging obstacle preventing drug penetration into the brain. Advances in dendrimer chemistry for central nervous system (CNS) treatments are presented here. Read More

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Diatom Allantoin Synthase Provides Structural Insights into Natural Fusion Protein Therapeutics.

ACS Chem Biol 2018 Aug 28;13(8):2237-2246. Epub 2018 Jun 28.

Department of Chemistry, Life Sciences, and Environmental Sustainability , University of Parma , 43124 , Parma , Italy.

Humans have lost the ability to convert urate into the more soluble allantoin with the evolutionary inactivation of three enzymes of the uricolytic pathway. Restoration of this function through enzyme replacement therapy can treat severe hyperuricemia and Lesch-Nyhan disease. Through a genomic exploration of natural gene fusions, we found that plants and diatoms independently evolved a fusion protein (allantoin synthase) complementing two human pseudogenes. Read More

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August 2018
2 Reads

Ultrasensitive cathode photoelectrochemical immunoassay based on TiO photoanode-enhanced 3D CuO nanowire array photocathode and signal amplification by biocatalytic precipitation.

Anal Chim Acta 2018 Oct 4;1027:33-40. Epub 2018 May 4.

State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210093, PR China.

Cathode photoelectrochemical immunoassay usually shows better anti-interference capacity toward real samples than anode photoelectrochemical immunoassay. However, its poor photocurrent response has greatly restricted the detection sensitivity. Herein, a promising ultrasensitive cathode photoelectrochemical immunoassay was developed based on TiO photoanode-enhanced 3D CuO nanowire array (NWA) photocathode, and coupled with signal amplification by horseradish peroxidase (HRP)-induced biocatalytic precipitation (BCP). Read More

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October 2018
6 Reads

Ag Nanoparticles Synthesized in R-phycoerythrin: Change in Bioconjugate Properties upon Ripening of Nanoparticles.

Curr Pharm Biotechnol 2018 ;19(5):422-427

Bach Institute of Biochemistry, Fundamentals of Biotechnology Federal Research Centre, Russian Academy of Sciences, Leninskii pr. 33, Moscow 119071, Russian Federation.

Background: Bioconjugates prepared from photoactive molecules and metal nanoparticles are suitable for the development of new optoelectronic devices and for theranostic applications in medicine.

Methods: We produced a bioconjugate of R-phycoerythrin (R-PE), a photosynthetic pigment of red algae, with Ag0 nanoparticles (Ag0 NPs) synthesized in its tunnel cavities by mixing aqueous solutions of AgNO3 and R-PE without exogenous reductant. In freshly prepared bioconjugate (Ag0⋅R-PE), the diameter of Ag0 NPs measured in electron microscopic images was 6. Read More

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November 2018
2 Reads

Ultrasensitive dual probe immunosensor for the monitoring of nicotine induced-brain derived neurotrophic factor released from cancer cells.

Biosens Bioelectron 2018 Sep 28;116:108-115. Epub 2018 May 28.

Department of Chemistry and Institute of BioPhysio Sensor Technology (IBST), Pusan National University, Busan 46241, South Korea. Electronic address:

Brain-derived neurotrophic factor (BDNF) was detected in the extracellular matrix of neuronal cells using a dual probe immunosensor (DPI), where one of them was used as a working and another bioconjugate loading probe. The working probe was fabricated by covalently immobilizing capture anti-BDNF (Cap Ab) on the gold nanoparticles (AuNPs)/conducting polymer composite layer. The bioconjugate probe was modified by drop casting a bioconjugate particles composed of conducting polymer self-assembled AuNPs, immobilized with detection anti-BDNF (Det Ab) and toluidine blue O (TBO). Read More

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September 2018

A Quantum Dot-Protein Bioconjugate That Provides for Extracellular Control of Intracellular Drug Release.

Bioconjug Chem 2018 Jul 18;29(7):2455-2467. Epub 2018 Jun 18.

Center for Bio/Molecular Science and Engineering, Code 6900 , U.S. Naval Research Laboratory , Washington , D.C. 20375 , United States.

The ability to control the intracellular release of drug cargos from nanobioconjugate delivery scaffolds is critical for the successful implementation of nanoparticle (NP)-mediated drug delivery. This is particularly true for hard NP carriers such as semiconductor quantum dots (QDs) and gold NPs. Here, we report the development of a QD-based multicomponent drug release system that, when delivered to the cytosol of mammalian cells, is triggered to release its drug cargo by the simple addition of a competitive ligand to the extracellular medium. Read More

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July 2018
3 Reads

Exploiting the Concept of Multivalency with Ga- and Zr-Labelled Fusarinine C-Minigastrin Bioconjugates for Targeting CCK2R Expression.

Contrast Media Mol Imaging 2018 10;2018:3171794. Epub 2018 Apr 10.

Department of Nuclear Medicine, Medical University Innsbruck, Anichstrasse 35, 6020 Innsbruck, Austria.

Cholecystokinin-2 receptors (CCK2R) are overexpressed in a variety of malignant diseases and therefore have gained certain attention for peptide receptor radionuclide imaging. Among extensive approaches to improve pharmacokinetics and metabolic stability of minigastrin (MG) based radioligands, the concept of multivalency for enhanced tumour targeting has not been investigated extensively. We therefore utilized fusarinine C (FSC) as chelating scaffold for novel mono-, di-, and trimeric bioconjugates for targeting CCK2R expression. Read More

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April 2018
8 Reads

Elastin-like Polypeptide (ELP) Charge Influences Self-Assembly of ELP-mCherry Fusion Proteins.

Biomacromolecules 2018 Jul 23;19(7):2517-2525. Epub 2018 May 23.

Department of Chemical Engineering , Massachusetts Institute of Technology , Cambridge , Massachusetts 02139 , United States.

Self-assembly of protein-polymer bioconjugates presents an elegant strategy for controlling nanostructure and orientation of globular proteins in functional materials. Recent work has shown that genetic fusion of globular protein mCherry to an elastin-like polypeptide (ELP) yields similar self-assembly behavior to these protein-polymer bioconjugates. In the context of studying protein-polymer bioconjugate self-assembly, the mutability of the ELP sequence allows several different properties of the ELP block to be tuned orthogonally while maintaining consistent polypeptide backbone chemistry. Read More

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July 2018
5 Reads

The Chemistry of Lyophilized Blood Products.

Bioconjug Chem 2018 Jul 13;29(7):2150-2160. Epub 2018 Jun 13.

Division of Trauma, Surgical Critical Care & Emergency Surgery , Perelman School of Medicine at the University of Pennsylvania , Philadelphia , Pennsylvania 19104 , United States.

With the development of new biologics and bioconjugates, storage and preservation have become more critical than ever before. Lyophilization is a method of cell and protein preservation by removing a solvent such as water from a substance followed by freezing. This technique has been used in the past and still holds promise for overcoming logistic challenges in safety net hospitals with limited blood banking resources, austere environments such as combat, and mass casualty situations where existing resources may be outstripped. Read More

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July 2018
11 Reads

Multifunctional, High Molecular Weight, Post-Translationally Modified Proteins through Oxidative Cysteine Coupling and Tyrosine Modification.

Bioconjug Chem 2018 Jun 22;29(6):1876-1884. Epub 2018 May 22.

Department of Chemical Engineering , Massachusetts Institute of Technology , 77 Massachusetts Avenue , Cambridge , Massachusetts 02139 , United States.

Glycoproteins and their mimics are challenging to produce via chemical or biological methods because of their long protein backbones and large number of polysaccharide side chains that form a densely grafted protein-polysaccharide brush architecture. Herein, we demonstrate a new approach to protein bioconjugate synthesis that can approach the molar mass and functionalization densities of natural glycoproteins such as mucins and aggrecans. In this method, a tyrosine-enriched protein sequence is engineered and synthesized in E. Read More

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June 2018
1 Read

Enhanced stability of L-asparaginase by its bioconjugation to poly(styrene-co-maleic acid) and Ecoflex nanoparticles.

IET Nanobiotechnol 2018 Jun;12(4):466-472

Department of Pharmaceutics, Faculty of Pharmacy and Novel Drug Delivery Systems Research Centre, Isfahan University of Medical Sciences, Isfahan, Iran.

Acute lymphoblastic leukemia (ALL) is the white blood cell cancer in children. L-asparaginase (L-ASNase) is one of the first drugs used in ALL treatment. Anti-tumor activity of L-ASNase is not specific and indicates limited stability in different biological environments, in addition to its quick clearance from blood. Read More

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June 2018
4 Reads

Preparation and preclinical evaluation of a Ga-labelled c(RGDfK) conjugate comprising the bifunctional chelator NODIA-Me.

EJNMMI Radiopharm Chem 2018 2;3(1). Epub 2018 May 2.

Department of Nuclear Medicine, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, Hugstetterstrasse 55, D-79106 Freiburg, Germany.

Background: We recently developed a chelating platform based on the macrocycle 1,4,7-triazacyclononane with up to three, five-membered azaheterocyclic arms for the development of Ga- and Cu-based radiopharmaceuticals. Here, a Ga-labelled conjugate comprising the bifunctional chelator NODIA-Me in combination with the αß-targeting peptide c(RGDfK) has been synthesized and characterized. The primary aim was to evaluate further the potential of our NODIA-Me chelating system for the development of Ga-labelled radiotracers. Read More

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May 2018
8 Reads

Protein purification by chemo-selective precipitation using thermoresponsive polymers.

Biopolymers 2018 Jun 6;109(6):e23222. Epub 2018 May 6.

College of Biological Science and Engineering, Fuzhou University, Fuzhou, 350002, China.

A recoverable and thermoresponsive polymer-protein bioconjugate is synthesized and employed in the purification of protein with free sulfhydryl groups. Initiator with disulphide was modified on the cysteine residue of the target protein. Poly(N-isopropylacrylamide) exhibiting a lower critical solution temperature was grown from the protein. Read More

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June 2018
3 Reads

Caged siRNAs with Single cRGD Modification for Photoregulation of Exogenous and Endogenous Gene Expression in Cells and Mice.

Biomacromolecules 2018 Jul 16;19(7):2526-2534. Epub 2018 May 16.

State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences and Center for Noncoding RNA Medicine , Peking University , No. 38, Xueyuan Rd , Beijing 100191 , China.

RNA interference (RNAi) mediated gene silencing holds significant promise in gene therapy. It is very important to manually regulate the activity of small interference RNAs (siRNAs) in the controllable mode. Here, we designed and synthesized a series of caged siRNAs through bioconjugation of cyclo(Arg-Gly-Asp-d-Phe-Lys) (cRGD) peptide to the 5' end of siRNA through a photolabile linker. Read More

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July 2018
6 Reads

New Formulation for the Delivery of Oligonucleotides Using "Clickable" siRNA-Polyisoprenoid-Conjugated Nanoparticles: Application to Cancers Harboring Fusion Oncogenes.

Bioconjug Chem 2018 Jun 11;29(6):1961-1972. Epub 2018 May 11.

Université Paris-Saclay , Laboratoire de Vectorologie et Thérapeutiques Anticancéreuses, UMR 8203, Villejuif , France - 94805.

The aim of the present study is to take advantage of the unique property of polyisoprenoid chains to adopt a compact molecular conformation and to use these natural and biocompatible lipids as nanocarriers of drugs to deliver siRNA. A new chemical strategy is applied here to conjugate squalene (SQ) and solanesol (SOLA) to siRNA consisting of an activated variant of the azide-alkyne Huisgen cycloaddition also known as copper-free (Cu-free) click chemistry. We conjugated siRNA against TMPRSS2-ERG, a fusion oncogene found in more than 50% of prostate cancers to SQ or SOLA. Read More

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June 2018
132 Reads

Combined electrochemiluminescent and electrochemical immunoassay for interleukin 6 based on the use of TiO mesocrystal nanoarchitectures.

Mikrochim Acta 2018 May 2;185(5):277. Epub 2018 May 2.

College of Chemistry and Chemical Engineering & College of Geographical Science, Fujian Normal University, Fuzhou, 350108, Fujian, China.

A dual-responsive sandwich-type immunosensor is described for the detection of interleukin 6 (IL-6) by combining electrochemiluminescent (ECL) and electrochemical (EC) detection based on the use of two kinds of TiO mesocrystal nanoarchitectures. A composite was prepared from TiO (anatase) mesocages (AMCs) and a carboxy-terminated ionic liquid (CTIL) and then placed on a glassy carbon electrode (GCE). In the next step, the ECL probe Ru(bpy)(II) and antibody against IL-6 (Ab) were immobilized on the GCE. Read More

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May 2018
1 Read

Synthesis and in vitro biochemical evaluation of oxime bond-linked daunorubicin-GnRH-III conjugates developed for targeted drug delivery.

Beilstein J Org Chem 2018 4;14:756-771. Epub 2018 Apr 4.

MTA-ELTE Research Group of Peptide Chemistry, Hungarian Academy of Sciences, Eötvös L. University, 1117 Budapest, Hungary.

Gonadotropin releasing hormone-III (GnRH-III), a native isoform of the human GnRH isolated from sea lamprey, specifically binds to GnRH receptors on cancer cells enabling its application as targeting moieties for anticancer drugs. Recently, we reported on the identification of a novel daunorubicin-GnRH-III conjugate (GnRH-III-[Lys(Bu), Lys(Dau=Aoa)] with efficient in vitro and in vivo antitumor activity. To get a deeper insight into the mechanism of action of our lead compound, the cellular uptake was followed by confocal laser scanning microscopy. Read More

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Cellular delivery of doxorubicin mediated by disulfide reduction of a peptide-dendrimer bioconjugate.

Int J Pharm 2018 Jul 27;545(1-2):64-73. Epub 2018 Apr 27.

Center for Bio/Molecular Science and Engineering, U.S. Naval Research Laboratory, Code 6900, Washington DC 20375, United States. Electronic address:

In this study, we developed a peptide-dendrimer-drug conjugate system for the pH-triggered direct cytosolic delivery of the cancer chemotherapeutic doxorubicin (DOX) using the pH Low Insertion Peptide (pHLIP). We synthesized a pHLIP-dendrimer-DOX conjugate in which a single copy of pHLIP displayed a generation three dendrimer bearing multiple copies of DOX via disulfide linkages. Biophysical analysis showed that both the dendrimer and a single DOX conjugate inserted into membrane bilayers in a pH-dependent manner. Read More

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July 2018
2 Reads

Polypeptides with High Zwitterion Density for Safe and Effective Therapeutics.

Angew Chem Int Ed Engl 2018 Jun 24;57(26):7743-7747. Epub 2018 May 24.

Department of Chemical Engineering, University of Washington, Seattle, WA, 98195, USA.

The commonly used "stealth material" poly(ethylene glycol) (PEG) effectively promotes the pharmacokinetics of therapeutic cargos while reducing their immune response. However, recent studies have suggested that PEG could induce adverse reactions, including the emergence of anti-PEG antibodies and tissue histologic changes. An alternative stealth material with no or less immunogenicity and organ toxicity is thus urgently needed. Read More

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June 2018
1 Read
11.260 Impact Factor

Exploring the DNA damaging potential of chitosan and citrate-reduced gold nanoparticles: Physicochemical approach.

Int J Biol Macromol 2018 Aug 24;115:801-810. Epub 2018 Apr 24.

Nano-bioconjugate Chemistry Lab, Cluster Innovation Centre, University of Delhi, Delhi, India. Electronic address:

Nanomaterials offer a wide range of biomedical applications including gene/drug delivery, biosensing and bioimaging. The cytotoxic and genotoxic potential of nanoparticles need to be thoroughly investigated before their biomedical usage. This study aims to investigate and compare the nanotoxicology of chitosan (CH-Au-Np) and citrate (CI-Au-Np) reduced gold nanoparticles via exploring their interaction with Calf thymus DNA (Ct-DNA) utilizing various physicochemical techniques. Read More

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August 2018
25 Reads

Quantitative and Label-Free Detection of Protein Kinase A Activity Based on Surface-Enhanced Raman Spectroscopy with Gold Nanostars.

Anal Chem 2018 May 26;90(10):6071-6080. Epub 2018 Apr 26.

Department of Biomedical Engineering , National University of Singapore , Singapore 117583.

The activity of extracellular protein kinase A (PKA) is known to be a biomarker for cancer. However, conventional PKA assays based on colorimetric, radioactive, and fluorometric techniques suffer from intensive labeling-related preparations, background interference, photobleaching, and safety concerns. While surface-enhanced Raman spectroscopy (SERS)-based assays have been developed for various enzymes to address these limitations, their use in probing PKA activity is limited due to subtle changes in the Raman spectrum with phosphorylation. Read More

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May 2018
1 Read

Electrochemical biointerfaces based on carbon nanotubes-mesoporous silica hybrid material: Bioelectrocatalysis of hemoglobin and biosensing applications.

Biosens Bioelectron 2018 Jul 4;111:144-151. Epub 2018 Apr 4.

INFIQC, Departamento de Físicoquimica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, 5000 Córdoba, Argentina. Electronic address:

We are reporting a novel biosensing platform based on a hybrid nanomaterial that combines the advantages of Nafion-coated multiwalled carbon nanotubes (MWCNTs) and mesoporous silica MCM41 nanoparticles functionalized with hemoglobin (Hb). MWCNTs-MCM41-Hb hybrid bioconjugate was characterized by scanning electron microscopy (SEM), UV-vis spectroscopy and electrochemical techniques after deposition at glassy carbon electrodes (GCE). The combination of the high surface area, biocompatibility and protein loading capacity of MCM41 nanoparticles and the high surface area and catalytic properties of MWCNTs allowed the direct electron transfer (DET) between Hb and the electrode surface. Read More

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July 2018
1 Read

Fluorescent microarray for multiplexed quantification of environmental contaminants in seawater samples.

Talanta 2018 Jul 16;184:499-506. Epub 2018 Mar 16.

Nanobiotechnology for diagnostics (Nb4D), Department of Chemical and Biomolecular Nanotechnology, Institute for Advanced Chemistry of Catalonia (IQAC) of the Spanish Council for Scientific Research (CSIC), Jordi Girona 18-26, 08034 Barcelona, Spain; CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Spain.

The development of a fluorescent multiplexed microarray platform able to detect and quantify a wide variety of pollutants in seawater is reported. The microarray platform has been manufactured by spotting 6 different bioconjugate competitors and it uses a cocktail of 6 monoclonal or polyclonal antibodies raised against important families of chemical pollutants such as triazine biocide (i.e. Read More

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Recyclable Thermoresponsive Polymer-β-Glucosidase Conjugate with Intact Hydrolysis Activity.

Biomacromolecules 2018 Jun 9;19(6):2286-2293. Epub 2018 May 9.

β-Glucosidase (BG) catalyzes the hydrolysis of cellobiose to glucose and is a rate-limiting enzyme in the conversion of lignocellulosic biomass to sugars toward biofuels. Since the cost of enzyme is a major contributor to biofuel economics, we report the bioconjugation of a temperature-responsive polymer with the highly active thermophilic β-glucosidase (B8CYA8) from Halothermothrix orenii toward improving enzyme recyclability. The bioconjugate, with a lower critical solution temperature (LCST) of 33 °C withstands high temperatures up to 70 °C. Read More

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June 2018
3 Reads
5.750 Impact Factor

Delivery of Proapoptotic Agents in Glioma Cell Lines by TSPO Ligand-Dextran Nanogels.

Int J Mol Sci 2018 Apr 11;19(4). Epub 2018 Apr 11.

Department of Pharmacy-Drug Sciences, University of Bari "Aldo Moro", Via Orabona, St. 4, 70125 Bari, Italy.

Translocator protein 18-kDa (TSPO) is a versatile mitochondrial target for molecular imaging and therapy. Moreover, selective TSPO ligands have been widely investigated for diagnostic purposes and explored to target drug delivery systems directed to cancer cells overexpressing TSPO. Indeed, poly(d,l-lactic-co-glycolic acid (PLGA) polymers and nanocarriers decorated with TSPO ligands are capable of transporting TSPO ligands inside cancer cells, inducing survival inhibition in cancer cells and producing mitochondrial morphology modification. Read More

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April 2018
2 Reads

Site-Specific Incorporation of Chemical Fluorescence on Live Enterovirus-71 Virion by Using an Organometallic Palladium Reagent To Monitor Virus Entry.

Chembiochem 2018 Apr 6. Epub 2018 Apr 6.

Collaborative Innovation Center of Biotherapy and MOE Laboratory of Protein Science, School of Medicine, Tsinghua University, Beijing, 100084, China.

Imaging live virus to monitor the viral entry process is essential to understand virus-host interactions during pathogen infection. However, methods for efficient labeling of live viruses, in particular labeling non-enveloped viruses and tracing virus entry processes, remain limited. Recently, labeling by using organometallic palladium reagents has provided a highly efficient and selective way to bioconjugate cysteines of virus proteins. Read More

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April 2018
3 Reads

Functionalized TiO nanoparticles labelled with Ac for targeted alpha radionuclide therapy.

J Nanopart Res 2018 20;20(3):83. Epub 2018 Mar 20.

1Institute of Nuclear Chemistry and Technology, Dorodna 16, 03-195 Warsaw, Poland.

The Ac radioisotope exhibits very attractive nuclear properties for application in radionuclide therapy. Unfortunately, the major challenge for radioconjugates labelled with Ac is that traditional chelating moieties are unable to sequester the radioactive daughters in the bioconjugate which is critical to minimize toxicity to healthy, non-targeted tissues. In the present work, we propose to apply TiO nanoparticles (NPs) as carrier for Ac and its decay products. Read More

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March 2018
1 Read

Catalytic characteristics and application of l-asparaginase immobilized on aluminum oxide pellets.

Int J Biol Macromol 2018 Jul 21;114:504-511. Epub 2018 Mar 21.

Enzyme Technology and Molecular Catalysis Laboratory, Department of Microbiology, Dr. Harisingh Gour Vishwavidyalaya (A Central University), Sagar, M.P. 470003, India. Electronic address:

l-asparaginase from Escherichia coli (l-ASNase) was covalently immobilized on aluminum oxide pellets (AlOPs) using a cross-linking agent, glutaraldehyde. Maximum immobilization yield (85.0%) was obtained after optimizing immobilization parameters using response surface methodology (RSM). Read More

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July 2018
8 Reads

In vitro and in vivo evaluation of the bifunctional chelator NODIA-Me in combination with a prostate-specific membrane antigen targeting vector.

Nucl Med Biol 2018 May 10;60:45-54. Epub 2018 Mar 10.

Department of Nuclear Medicine, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, Hugstetterstrasse 55, D-79106 Freiburg, Germany. Electronic address:

Introduction: We recently developed a chelating platform based on the macrocycle 1,4,7-triazacyclononane with up to three five-membered azaheterocyclic arms for complexation of the PET nuclides gallium-68 and copper-64. The main objective of this study was to evaluate the stability and pharmacokinetics of Ga- and Cu-complexes of the bifunctional chelator NODIA-Me 1 covalently bound to a PSMA targeting vector in vivo.

Methods: NODIA-Me 1 was conjugated to the PSMA targeting Glu-NH-CO-NH-Lys moiety to give the bioconjugate NODIA-Me-NaI-Ahx-PSMA 4. Read More

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May 2018
4 Reads