6 results match your criteria Biochip Journal[Journal]

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Multiscale Biofluidic and Nanobiotechnology Approaches for Treating Sepsis in Extracorporeal Circuits.

Authors:
Joo H Kang

Biochip J 2020 Mar 12:1-9. Epub 2020 Mar 12.

Department of Biomedical Engineering, Ulsan National Institute of Science and Technology (UNIST), 50, UNIST-gil, Ulsan, 44919 Republic of Korea.

Infectious diseases and their pandemics periodically attract public interests due to difficulty in treating the patients and the consequent high mortality. Sepsis caused by an imbalanced systemic inflammatory response to infection often leads to organ failure and death. The current therapeutic intervention mainly includes "the sepsis bundles," antibiotics (antibacterial, antiviral, and antifungal), intravenous fluids for resuscitation, and surgery, which have significantly improved the clinical outcomes in past decades; however, the patients with fulminant sepsis are still in desperate need of alternative therapeutic approaches. Read More

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http://dx.doi.org/10.1007/s13206-020-4106-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7095347PMC

A Simple and Multiplex Loop-Mediated Isothermal Amplification (LAMP) Assay for Rapid Detection of SARS-CoV.

Biochip J 2019 11;13(4):341-351. Epub 2019 Nov 11.

6Department of Microbiology and Immunology, Seoul National University College of Medicine, Seoul, South Korea.

The current diagnosis of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) relies on laboratory-based tests since its clinical features are nonspecific, unlike other respiratory pathogens. Therefore, the development of a rapid and simple method for on-site detection of SARS-CoV is crucial for the identification and prevention of future SARS outbreaks. In this study, a simple colorimetric and multiplex loop-mediated isothermal amplification (LAMP) assay was developed to rapid screening of severe acute respiratory syndrome-associated coronavirus (SARS-CoV). Read More

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http://dx.doi.org/10.1007/s13206-019-3404-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7097549PMC
November 2019
1.090 Impact Factor

Direct buffer composition of blood pre-process for nucleic acid based diagnostics.

Biochip J 2017 12;11(4):255-261. Epub 2017 Dec 12.

School of Integrative Engineering, Chung-Ang University, Heuksukdong, Dongjak-gu, Seoul, 06974 Republic of Korea.

Recently, a variety of methods, so called "direct buffer", have been developed to utilize nucleic acid in the blood for the measurement of infectious bacteria and virus without any equipment in the field. In here, we first investigated the individual and combinatory effects of candidate chemicals which might be composed of the direct buffer on the PCR inhibition reduction of main compositions in whole blood. The long and short PEGs, NaSO and GuSCN were selected as representative kosmotropic and chaotropic salts, respectively. Read More

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http://dx.doi.org/10.1007/s13206-017-1401-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7097592PMC
December 2017

Rolling circle amplification as isothermal gene amplification in molecular diagnostics.

Biochip J 2016 29;10(4):262-271. Epub 2016 Jul 29.

Department of Bioscience and Biotechnology, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul, 05029 Korea.

Rolling circle amplification (RCA) developed in the mid-1990s has been widely used as an efficient isothermal DNA amplification process for molecular diagnosis. This enzymatic process amplifies target DNA sequences with high fidelity and specificity by using the strand displacing DNA polymerases. The product of RCA is long single-stranded DNA that contains tandem repeat of target sequence. Read More

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http://dx.doi.org/10.1007/s13206-016-0402-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7096790PMC

Passive micromixer using by convection and surface tension effects with air-liquid interface.

Biochip J 2013 Dec;7(4):361-366

Wisconsin Institute for Medical Research, University of Wisconsin-Madison, Madison, WI, 53705 USA ; Wisconsin Institute for Discovery, University of Wisconsin-Madison, Madison, WI, 53715 USA.

This article describes a passive micromixer that utilizes an air-liquid interface and surface tension effects to enhance fluid mixing via convection and Marangoni effects. Performance of the microfluidic component is tested within a passive-pumping-based device that consists of three microchannels connected in succession using passive micro-mixers. Mixing was quantified at 5 key points along the length of the device using microscope images of patterned streams of Alexa 488 fluorescent-dyed water and pure DI water flowing through the device. Read More

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http://dx.doi.org/10.1007/s13206-013-7407-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4120973PMC
December 2013
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A comparison of oligonucleotide-based microarray and real-time PCR for the detection of sexually transmitted infections.

Biochip J 2013 20;7(1):68-74. Epub 2013 Mar 20.

Molecular Diagnostic Research Institute, Bioneer Corporation, Daejeon, Korea.

Many diagnostic methods for sexually transmitted infections (STIs) have been developed. Because various infection agents are associated with STIs, and because infected persons sometimes show no symptoms, the diagnosis of STIs using nucleic acid amplification tests(NAATs) has required not only simultaneous multi-targeting, but also sensitive detection. Here, we compare microarray and real-time PCR for the detection of three common STIs agents, , , and , using human urine samples. Read More

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http://dx.doi.org/10.1007/s13206-013-7111-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7096812PMC
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