52 results match your criteria Biochimie open[Journal]


Vanadate inhibits transcription of the rat insulin receptor gene via a proximal sequence of the 5'flanking region.

Biochim Open 2018 Dec 10;7:26-32. Epub 2018 Oct 10.

INSERM U1016, Institut Cochin, CNRS UMR8104, Université Paris Descartes, Sorbonne Paris Cité, Paris, France.

Vanadate, a protein tyrosine phosphatase inhibitor which elicits insulin-like effects, has previously been shown to inhibit expression of the insulin receptor gene at the transcriptional level in rat hepatoma cells. In an attempt to identify the DNA sequence and transcription factors potentially involved in this effect, a fragment of the proximal 5'flanking region of the IR gene (-1143/-252 upstream the ATG codon) has been cloned and functionally characterized. RNase protection allowed the identification of several transcription start sites in the conserved region of the gene, among which two major sites at -455 and -396. Read More

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http://dx.doi.org/10.1016/j.biopen.2018.09.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6205930PMC
December 2018
1 Read

Retroviral nucleocapsid proteins and DNA strand transfers.

Biochim Open 2018 Dec 20;7:10-25. Epub 2018 Jul 20.

LBPA, ENS Paris-Saclay, UMR 8113, CNRS, Université Paris-Saclay, 61 Avenue du Président Wilson, 94235, Cachan, France.

An infectious retroviral particle contains 1000-1500 molecules of the nucleocapsid protein (NC) that cover the diploid RNA genome. NC is a small zinc finger protein that possesses nucleic acid chaperone activity that enables NC to rearrange DNA and RNA molecules into the most thermodynamically stable structures usually those containing the maximum number of base pairs. Thanks to the chaperone activity, NC plays an essential role in reverse transcription of the retroviral genome by facilitating the strand transfer reactions of this process. Read More

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http://dx.doi.org/10.1016/j.biopen.2018.07.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6088434PMC
December 2018
115 Reads

AhR signaling pathways and regulatory functions.

Biochim Open 2018 Dec 11;7:1-9. Epub 2018 Jun 11.

INSERM UMR-S 1124, Toxicologie Pharmacologie et Signalisation Cellulaire, 45 rue des Saints-Pères, 75006 Paris, France.

Animals and humans are exposed each day to a multitude of chemicals in the air, water and food. They have developed a battery of enzymes and transporters that facilitate the biotransformation and elimination of these compounds. Moreover, a majority of these enzymes and transporters are inducible due to the activation of xenobiotic receptors which act as transcription factors for the regulation of their target genes (such as xenobiotic metabolizing enzymes, see below §4 for the AhR). Read More

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https://linkinghub.elsevier.com/retrieve/pii/S22140085183000
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http://dx.doi.org/10.1016/j.biopen.2018.05.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6039966PMC
December 2018
5 Reads

Isoprostanes as markers for muscle aging in older athletes.

Biochim Open 2018 Jun 15;6:1-8. Epub 2017 Dec 15.

Institute of Metabolic and Cardiovascular Diseases, Joint Research Unit 1048 INSERM Adipolab Unit - Paul Sabatier University, Toulouse, France.

Introduction: Production of isoprostanes (IsoPs) is enhanced after acute, intense, and prolonged exercise, in untrained subjects. This effect is greater in older subjects. The present study aims to delineate the profile of acute-exercise-induced IsoPs levels in young and older endurance-trained subjects. Read More

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http://dx.doi.org/10.1016/j.biopen.2017.12.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5991887PMC
June 2018
17 Reads

Lack of current observed in HEK293 cells expressing NALCN channels.

Biochim Open 2018 Jun 5;6:24-28. Epub 2018 Feb 5.

Department of Biological Sciences, University of Manitoba, Winnipeg, MB, R3T 2N2, Canada.

The sodium leak channel NALCN is poorly understood, but is reported as a Na-permeable, nonselective cation leak channel which regulates resting membrane potential and electrical excitability. Previous work has indicated that NALCN currents can be stimulated by activation of several G protein coupled receptors, including the M3 muscarinic receptor. We undertook a study using voltage clamp electrophysiology to investigate NALCN currents. Read More

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http://dx.doi.org/10.1016/j.biopen.2018.01.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5991895PMC

Glycan binding profile of a fucolectin-related protein (FRP) encoded by the SP2159 gene of .

Biochim Open 2018 Jun 23;6:17-23. Epub 2017 Dec 23.

Department of Biochemistry and Molecular Biology, Tulane University, School of Medicine, 1430 Tulane Ave., New Orleans, LA 70112, USA.

The recombinant fucolectin-related protein (FRP) of unknown function, encoded by the SP2159 gene of was expressed in . In this study, its glycan-recognition epitopes and their binding potencies were examined by enzyme-linked lectinosorbent and inhibition assays. The results indicate that FRP reacted strongly with human blood group and l-Fucα1→2-active glycotopes and in their polyvalent (super) forms. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S22140085173001
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http://dx.doi.org/10.1016/j.biopen.2017.12.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5991896PMC
June 2018
16 Reads

Herbal genomics as tools for dissecting new metabolic pathways of unexplored medicinal plants and drug discovery.

Biochim Open 2018 Jun 10;6:9-16. Epub 2018 Jan 10.

Kalpana Chawla Center for Space and Nanosciences, Indian Institute of Chemical Biology (retd.), Kolkata, 700032, India.

Herbal drugs, on which 80% of the world's population rely, are relatively safe over conventional drugs. Conventional drugs are costly, have serious side effects and hence over the past few decades researchers have focused on drug discovery from herbal medicines or botanical sources. The majority of new herbal drugs have been generated from secondary metabolites (alkaloids, terpenoids and phenolic compounds) of plant metabolism. Read More

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http://dx.doi.org/10.1016/j.biopen.2017.12.003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5991880PMC
June 2018
9 Reads

Comparison of antimicrobial activities of natural essential oils and synthetic fragrances against selected environmental pathogens.

Biochim Open 2017 Dec 13;5:8-13. Epub 2017 Sep 13.

4Life Holdings, LLC, 9850 South 300 West, Sandy, UT 84070, USA.

Plant essential oils (EOs) are known to inhibit the growth of bacteria and fungi. Whether these antimicrobial effects are comparable to synthetic household products is less clear. Furthermore, limited research is available on the potential additive effect of blending EOs. Read More

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http://dx.doi.org/10.1016/j.biopen.2017.09.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5805554PMC
December 2017
4 Reads

Chemical composition analysis and biological activities of ten essential oils in human skin cells.

Biochim Open 2017 Dec 26;5:1-7. Epub 2017 Apr 26.

dōTERRA International, LLC, 389 S. 1300 W., Pleasant Grove, UT 84062, USA.

Research on the biological effects of essential oils on human skin cells is scarce. In the current study, we primarily explored the biological activities of 10 essential oils (nine single and one blend) in a pre-inflamed human dermal fibroblast system that simulated chronic inflammation. We measured levels of proteins critical for inflammation, immune responses, and tissue-remodeling processes. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S22140085173001
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http://dx.doi.org/10.1016/j.biopen.2017.04.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5805555PMC
December 2017
5 Reads

Human and mouse microarrays-guided expression analysis of membrane protein trafficking-related genes in MDCK cells, a canine epithelial model for apical and basolateral differential protein targeting.

Biochim Open 2017 Jun 30;4:119-126. Epub 2017 Apr 30.

Department of Exercise and Nutrition Sciences, University at Buffalo, Buffalo, NY 14214, USA.

MDCK cells are widely used to study the differential targeting of membrane transporters to apical and basolateral membrane but its canine origin limited the commercial tools available for the analysis of protein trafficking machinery. Because apical and basolateral membranes are only found in differentiated epithelial cells, genes critical for differential targeting may be specifically up-regulated upon MDCK cell differentiation. To search for these genes, a cross-species screening strategy was used. Read More

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http://dx.doi.org/10.1016/j.biopen.2017.04.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5801818PMC
June 2017
1 Read

The effect of endurance training with and without vitamin E on expression of p53 and PTEN tumor suppressing genes in prostate glands of male rats.

Biochim Open 2017 Jun 1;4:112-118. Epub 2017 Apr 1.

Department of Physical Education & Sport Sciences, University of Birjand, Avini Blvd, Birjand, South Khorasan Province, Iran.

The aim of this study was to investigate the effect of endurance training with and without vitamin E on the expression of p53 and Phosphatase and tension homolog (PTEN) tumor suppressor genes of prostate glands in male rats. For this purpose, 50 Sprague-Dawley male rats were randomly assigned into 5 groups: (1) control group (CON, n = 10), (2) sham (S, n = 10), (3) endurance training (ET, n = 10), (4) endurance training + vitamin E (ET + VE, n = 10), (5) vitamin E (VE, n = 10). Endurance training protocol was implemented for 6 weeks, 6 days per week, in accordance with the overload principle. Read More

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http://dx.doi.org/10.1016/j.biopen.2017.03.005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5801830PMC

Lemongrass () essential oil demonstrated anti-inflammatory effect in pre-inflamed human dermal fibroblasts.

Biochim Open 2017 Jun 21;4:107-111. Epub 2017 Mar 21.

dōTERRA International, LLC, 389 S. 1300 W., Pleasant Grove, UT 84062, USA.

Lemongrass () essential oil (LEO), which has citral as its main component, has exhibited anti-inflammatory effect in both animal and human cells. In this study, we evaluated the anti-inflammatory activity of a commercially available LEO in pre-inflamed human dermal fibroblasts. We first studied the impact of LEO on 17 protein biomarkers that are critically associated with inflammation and tissue remodeling. Read More

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http://dx.doi.org/10.1016/j.biopen.2017.03.004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5801909PMC
June 2017
5 Reads

Novel proteins from proteomic analysis of the trunk disease fungus (Botryosphaeriaceae).

Biochim Open 2017 Jun 14;4:88-98. Epub 2017 Mar 14.

Centro de Investigación Científica y de Educación Superior de Ensenada (CICESE), Carretera Ensenada-Tijuana 3918, Zona Playitas, 22860 Ensenada, B.C., Mexico.

Many basic science questions remain regarding protein functions in the pathogen: host interaction, especially in the trunk disease fungi family, the Botryosphaeriaceae, which are a global problem for economically important plants, especially fruiting trees. Proteomics is a highly useful technology for studying protein expression and for discovering novel proteins in unsequenced and poorly annotated organisms. Current fungal proteomics approaches involve 2D SDS-PAGE and extensive, complex, protein extraction methodologies. Read More

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http://dx.doi.org/10.1016/j.biopen.2017.03.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5802045PMC
June 2017
2 Reads

Phosphorylation of purified mitochondrial Voltage-Dependent Anion Channel by c-Jun N-terminal Kinase-3 modifies channel voltage-dependence.

Biochim Open 2017 Jun 10;4:78-87. Epub 2017 Mar 10.

Department of Biophysics, University of Delhi South Campus, India.

Voltage-Dependent Anion Channel (VDAC) phosphorylated by c-Jun N-terminal Kinase-3 (JNK3) was incorporated into the bilayer lipid membrane. Single-channel electrophysiological properties of the native and the phosphorylated VDAC were compared. The open probability versus voltage curve of the native VDAC displayed symmetry around the voltage axis, whereas that of the phosphorylated VDAC showed asymmetry. Read More

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http://dx.doi.org/10.1016/j.biopen.2017.03.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5802065PMC
June 2017
3 Reads

Anti-inflammatory, tissue remodeling, immunomodulatory, and anticancer activities of oregano () essential oil in a human skin disease model.

Biochim Open 2017 Jun 3;4:73-77. Epub 2017 Mar 3.

dōTERRA International, LLC, 389 S. 1300 W., Pleasant Grove, UT 84062, USA.

The use of oregano () essential oil (OEO) has become popular in skin care products. However, scientific research regarding its effects on human skin cells is scarce. In this study, we investigated the biological activity of a commercially available OEO, which is high in carvacrol content, in a human skin cell disease model. Read More

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http://dx.doi.org/10.1016/j.biopen.2017.02.005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5801825PMC
June 2017
2 Reads

Purified horse milk exosomes contain an unpredictable small number of major proteins.

Biochim Open 2017 Jun 1;4:61-72. Epub 2017 Mar 1.

SB RAS Institute of Chemical Biology and Fundamental Medicine, 8 Lavrentiev Ave., Novosibirsk 630090, Russia.

Exosomes are 40-100 nm nanovesicles containing RNA and different proteins. Exosomes containing proteins, lipids, mRNAs, and microRNAs are important in intracellular communication and immune function. Exosomes from different sources are usually obtained by combination of centrifugation and ultracentrifugation and according to published data can contain from a few dozens to thousands of different proteins. Read More

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http://dx.doi.org/10.1016/j.biopen.2017.02.004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5801828PMC
June 2017
4 Reads

Arborvitae () essential oil significantly inhibited critical inflammation- and tissue remodeling-related proteins and genes in human dermal fibroblasts.

Biochim Open 2017 Jun 20;4:56-60. Epub 2017 Feb 20.

dōTERRA International, LLC, 389 S. 1300 W., Pleasant Grove, UT 84062, USA.

Arborvitae () essential oil (AEO) is becoming increasingly popular in skincare, although its biological activity in human skin cells has not been investigated. Therefore, we sought to study AEO's effect on 17 important protein biomarkers that are closely related to inflammation and tissue remodeling by using a pre-inflamed human dermal fibroblast culture model. AEO significantly inhibited the expression of vascular cell adhesion molecule 1 (VCAM-1), intracellular cell adhesion molecule 1 (ICAM-1), interferon gamma-induced protein 10 (IP-10), interferon-inducible T-cell chemoattractant (I-TAC), monokine induced by interferon gamma (MIG), and macrophage colony-stimulating factor (M-CSF). Read More

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http://dx.doi.org/10.1016/j.biopen.2017.02.003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5801906PMC
June 2017
3 Reads

Putative roles of mitochondrial Voltage-Dependent Anion Channel, Bcl-2 family proteins and c-Jun N-terminal Kinases in ischemic stroke associated apoptosis.

Biochim Open 2017 Jun 16;4:47-55. Epub 2017 Feb 16.

Department of Biophysics, University of Delhi South Campus, New Delhi, India.

There is a constant need for better stroke treatments. Neurons at the periphery of an ischemic stroke affected brain tissue remains metabolically active for several hours or days after stroke onset. They later undergo mitochondrion-mediated apoptosis. Read More

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http://dx.doi.org/10.1016/j.biopen.2017.02.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5802046PMC
June 2017
1 Read

JNK3 phosphorylates Bax protein and induces ability to form pore on bilayer lipid membrane.

Biochim Open 2017 Jun 11;4:41-46. Epub 2017 Feb 11.

Department of Biophysics, University of Delhi South Campus, India.

Bax is a pro-apoptotic cytosolic protein. In this work native (unphosphorylated) and JNK3 phosphorylated Bax proteins are studied on artificial bilayer membranes for pore formation. Phosphorylated Bax formed pore on the bilayer lipid membrane whereas native one does not. Read More

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http://dx.doi.org/10.1016/j.biopen.2017.02.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5801821PMC
June 2017
1 Read

The nature of the rate-limiting step of blue multicopper oxidases: Homogeneous studies heterogeneous.

Biochim Open 2017 Jun 3;4:36-40. Epub 2017 Feb 3.

CNRS, CRPP, UPR 8641, F-33600 Pessac, France.

Multicopper oxidases (MCOs) catalyzed two half reactions (linked by an intramolecular electron transfer) through a Ping-Pong mechanism: the substrate oxidation followed by the O reduction. MCOs have been characterized in details in solution or immobilized on electrode surfaces. The nature of the rate-limiting steps, which is controversial in the literature, is discussed in this mini review for both cases. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S22140085173000
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http://dx.doi.org/10.1016/j.biopen.2017.01.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5801829PMC
June 2017
6 Reads

Biological activities of frankincense essential oil in human dermal fibroblasts.

Biochim Open 2017 Jun 3;4:31-35. Epub 2017 Feb 3.

dōTERRA International, LLC, 389 S. 1300 W, Pleasant Grove, UT 84062, USA.

Although frankincense essential oil (FREO) has become increasingly popular in skin care, research on its biological activities in human skin cells is scarce, if not completely absent. In the current study, we explored the biological activities of FREO in pre-inflamed human dermal fibroblasts by analyzing the levels of 17 important protein biomarkers pertinent to inflammation and tissue remodeling. FREO exhibited robust anti-proliferative activity in these skin cells. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S22140085173000
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http://dx.doi.org/10.1016/j.biopen.2017.01.003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5801908PMC
June 2017
2 Reads

Liver and Metformin: Lessons of a fructose diet in mice.

Biochim Open 2017 Jun 3;4:19-30. Epub 2017 Feb 3.

Laboratory of Morphometry, Metabolism, and Cardiovascular Diseases, Biomedical Center, Institute of Biology, State University of Rio de Janeiro, Brazil.

Studies show that the continuous consumption of fructose can lead to nonalcoholic fatty liver disease (NAFLD) and steatohepatitis. We aimed to investigate the role of Metformin in an animal model of liver injury caused by fructose intake, focusing on the molecular markers of lipogenesis, beta-oxidation, and antioxidant defenses. Male three months old C57BL/6 mice were divided into control group (C) and fructose group (F, 47% fructose), maintained for ten weeks. Read More

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http://dx.doi.org/10.1016/j.biopen.2017.01.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5801827PMC
June 2017
3 Reads

Initiative action of tumor-associated macrophage during tumor metastasis.

Biochim Open 2017 Jun 5;4:8-18. Epub 2017 Jan 5.

Department of Oral and Maxillofacial Surgery, The Affiliated Hospital of Stomatology, Chongqing Medical University, No. 426 Songshibei Road, Yubei District, Chongqing 401147, China.

Tumor-associated macrophages (TAMs) are a significant component of the microenvironment of any solid tumors in the majority of cancers, associated with unfavorable prognosis. TAMs emerge as attractive targets for therapeutic strategies aimed at reprogramming their protumor phenotype into an effective antitumor activity. In this review article, we present an overview of mechanisms responsible for TAMs recruitment and highlight the roles of TAMs in the regulation of tumor angiogenesis, invasion, metastasis, immunosuppression, and chemotherapeutic resistance. Read More

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http://dx.doi.org/10.1016/j.biopen.2016.11.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5801826PMC
June 2017
3 Reads

Subcellular localization of the five members of the human steroid 5α-reductase family.

Biochim Open 2017 Jun 21;4:99-106. Epub 2017 Mar 21.

Dept. of Biochemical Sciences, Sapienza University of Rome, P.le A.Moro 5, 00185, Rome, Italy.

In humans the steroid 5alpha-reductase (SRD5A) family comprises five integral membrane enzymes that carry out reduction of a double bond in lipidic substrates: Δ-3-keto steroids, polyprenol and trans-enoyl CoA. The best-characterized reaction is the conversion of testosterone into the more potent dihydrotestosterone carried out by SRD5A1-2. Some controversy exists on their possible nuclear or endoplasmic reticulum localization. Read More

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http://dx.doi.org/10.1016/j.biopen.2017.03.003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5656259PMC
June 2017
2 Reads

Development of a specific index to detect malnutrition in athletes: Validity in weight class or intermittent fasted athletes.

Biochim Open 2017 Jun 23;4:1-7. Epub 2016 Nov 23.

EA 7329, Paris Descartes University, Paris, France.

Fasted or weight-category athletes manage their training under strict diet conditions that could impair the stress-recovery balance and result in acute or chronic fatigue. However, to date, no validated biomarker are available to quantify this phenomena. The aim of this study was to assess the validity of a specific index combining plasma albumin and weight change to detect nutrition-related risks of fatigue increase and under-performance in athletes experiencing particular nutritional conditions. Read More

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http://dx.doi.org/10.1016/j.biopen.2016.10.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5802101PMC
June 2017
46 Reads

Inhibition of the MAPK pathway alone is insufficient to account for all of the cytotoxic effects of naringenin in MCF-7 breast cancer cells.

Biochim Open 2016 Dec 22;3:64-71. Epub 2016 Nov 22.

Department of Biology, University of North Carolina at Greensboro, Greensboro, NC 27412, USA.

Estrogen receptor (ER) antagonists such as tamoxifen (Tam) have been used successfully to treat ER+ breast cancers for more than 30 years. Unfortunately, long term use of Tam can result in resistance. Tam resistance is associated with the activation of growth factor signaling pathways that promote cell proliferation and survival. Read More

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http://dx.doi.org/10.1016/j.biopen.2016.09.004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5801822PMC
December 2016

Green tea polyphenols affect invasiveness of human gastric MKN-28 cells by inhibition of LPS or TNF-α induced Matrix Metalloproteinase-9/2.

Biochim Open 2016 Dec 5;3:56-63. Epub 2016 Nov 5.

Dipartimento di Medicina Clinica e Chirurgica, Università di Napoli Federico II, Via S. Pansini 5, 80131 Napoli, Italy.

Several studies demonstrated a correlation between green tea consumption and a reduced cancer risk. Among different components, green tea polyphenols have been identified as molecules responsible for the beneficial effects showed by the green tea against oxidative stress and cell invasiveness. In this study, we investigated the effects of green tea polyphenol extracts (GTPs) in human gastric MKN-28 cell line. Read More

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http://dx.doi.org/10.1016/j.biopen.2016.10.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5802102PMC
December 2016
3 Reads

Dimethyl-Benz(a)anthracene: A mammary carcinogen and a neuroendocrine disruptor.

Biochim Open 2016 Dec 8;3:49-55. Epub 2016 Oct 8.

INSERM UMR-S 1124, Université Paris Descartes, PRES Sorbonne Paris Cité, Pharmacologie Toxicologie et Signalisation Cellulaire, Faculté des Sciences Fondamentales et Biomédicales, 45 rue des Saints Pères, 75006 Paris, France.

Polycyclic Aromatic Hydrocarbons (PAHs) are potent carcinogens. Among these, dimethylbenz(a)anthracene (DMBA) is well known for its capacity to induce mammary carcinomas in female Sprague-Dawley (SD) rats. Ovariectomy suppresses the susceptibility of this model to DMBA, thus suggesting that the inducible action of the carcinogen depends on ovarian hormones. Read More

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http://dx.doi.org/10.1016/j.biopen.2016.09.003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5801823PMC
December 2016
1 Read

Thyroid and reproductive hormones disruption as well as kallikrein-3 level in dimethyl nitrosamine-induced toxicity: Effects of ascorbate treatment in male wistar rats.

Biochim Open 2016 Dec 28;3:40-46. Epub 2016 Sep 28.

Department of Biochemistry, College of Biosciences, Federal University of Agriculture Abeokuta, Abeokuta, Nigeria.

Information on dimethyl nitrosamine (DMN)-induced toxicity on endocrine functions is still scanty. This study therefore investigated the outcomes of DMN-induced toxicity on endocrine (thyroid and reproductive) functions, as well as kallikrein-3 level, and effects of ascorbate treatments in male wistar rats. Thirty animals divided into six groups of five rats each were used. Read More

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http://dx.doi.org/10.1016/j.biopen.2016.09.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5801907PMC
December 2016
4 Reads

Mobile phones electromagnetic radiation and NAD+-dependent isocitrate dehydrogenase as a mitochondrial marker in asthenozoospermia.

Biochim Open 2016 Dec 22;3:47-48. Epub 2016 Sep 22.

Oral and Maxillofacial Radiology Department, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.

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http://dx.doi.org/10.1016/j.biopen.2016.09.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5801824PMC
December 2016

Mobile phones electromagnetic radiation and NAD-dependent isocitrate dehydrogenase as a mitochondrial marker in asthenozoospermia.

Biochim Open 2016 Dec 25;3:19-25. Epub 2016 Jul 25.

Department of Medical Biochemistry, Faculty of Medicine, Suez Canal University, P.O. 41522, Ismailia, Egypt.

NAD-dependent Isocitrate Dehydrogenase (NAD-IDH) could be one of the cell phone radiation targets. Enzyme activity alteration may lead to decline in sperm motility during radio-frequency electromagnetic waves (RF-EMW) exposure. The current case control study aimed to investigate the possible relationship between mitochondrial NAD-IDH activity in human seminal plasma and sperm motility among asthenozoospermic cellular phone users. Read More

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http://dx.doi.org/10.1016/j.biopen.2016.07.003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5802047PMC
December 2016
1 Read

Structural properties of the linkers connecting the N- and C- terminal domains in the MocR bacterial transcriptional regulators.

Biochim Open 2016 Dec 20;3:8-18. Epub 2016 Jul 20.

Dipartimento di Scienze biochimiche "A. Rossi Fanelli", Sapienza Università di Roma, 00185 Roma, Italy.

Peptide inter-domain linkers are peptide segments covalently linking two adjacent domains within a protein. Linkers play a variety of structural and functional roles in naturally occurring proteins. In this work we analyze the sequence properties of the predicted linker regions of the bacterial transcriptional regulators belonging to the recently discovered MocR subfamily of the GntR regulators. Read More

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http://dx.doi.org/10.1016/j.biopen.2016.07.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5801912PMC
December 2016

Molecular characterization and analysis of the porcine gene.

Biochim Open 2016 Dec 19;3:26-39. Epub 2016 Jul 19.

Department of Molecular Biology and Genetics, Aarhus University, Blichers Allé 20, DK-8830 Tjele, Denmark.

Orphan receptor NURR1 (also termed NR4A2) belongs to the nuclear receptor superfamily and functions as a regulatory factor of differentiation, migration, maturation and maintenance of mesencephalic dopaminergic neurons. NURR1 plays an important role in nigrostriatal dopamine neuron development and is therefore implicated in the pathogenesis of neurodegenerative diseases linked to the dopamine system of the midbrain. Here we report the isolation and characterization of porcine cDNA. Read More

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http://dx.doi.org/10.1016/j.biopen.2016.07.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5801910PMC
December 2016
4 Reads

Characterization of the activity of β-galactosidase from and in fixed and non-fixed tissues.

Biochim Open 2016 Dec 22;3:1-7. Epub 2016 Jun 22.

Department of Biomolecular Science, Faculty of Science, Toho University, 2-2-1 Miyama, Funabashi, Chiba 274-8510, Japan.

β-Galactosidase encoded by the gene, is widely used as a reporter molecule in molecular biology in a wide variety of animals. β-Galactosidase retains its enzymatic activity in cells or tissues even after fixation and can degrade X-Gal, a frequently used colormetric substrate, producing a blue color. Therefore, it can be used for the activity staining of fixed tissues. Read More

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http://dx.doi.org/10.1016/j.biopen.2016.06.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5801911PMC
December 2016
2 Reads

The proto-oncogenic protein TAL1 controls TGF-β1 signaling through interaction with SMAD3.

Biochim Open 2016 Jun 14;2:69-78. Epub 2016 May 14.

Univ Lyon, ENS de Lyon, Univ Claude Bernard, CNRS UMR 5239, INSERM U1210, Laboratory of Biology and Modelling of the Cell, 46 Allée d'Italie Site Jacques Monod, F-69007, Lyon, France.

TGF-β1 is involved in many aspects of tissue development and homeostasis including hematopoiesis. The TAL1 transcription factor is also an important player of this latter process and is expressed very early in the myeloid and erythroid lineages. We previously established a link between TGF-β1 signaling and TAL1 by showing that the cytokine was able to induce its proteolytic degradation by the ubiquitin proteasome pathway. Read More

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http://dx.doi.org/10.1016/j.biopen.2016.05.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5889486PMC

Anti-inflammatory and antinociceptive activities of leaf lectin.

Biochim Open 2016 Jun 24;2:62-68. Epub 2016 Mar 24.

Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Pernambuco, UFPE, Av. Prof. Moraes Rego, 1235, Cidade Universitária, CEP 50.670-901 Recife, PE, Brazil.

A galactose-specific lectin from leaves (BmoLL) has been purified through ammonium sulfate fractionation followed by guar gel affinity chromatography column. This study aimed to evaluate the potential anti-inflammatory and antinociceptive activity of pure BmoLL in mice. Anti-inflammatory activity was evaluated by 1% carrageenan-induced inflammation in mice treated with BmoLL. Read More

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http://dx.doi.org/10.1016/j.biopen.2016.03.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5889483PMC
June 2016
1 Read

Association of human mitochondrial lysyl-tRNA synthetase with HIV-1 GagPol does not require other viral proteins.

Biochim Open 2016 Jun 5;2:52-61. Epub 2016 Mar 5.

Institute for Integrative Biology of the Cell (I2BC), Université Paris-Saclay, CEA, CNRS, Université Paris-Sud, 1 avenue de la Terrasse, 91190 Gif-sur-Yvette, France.

In human, the cytoplasmic (cLysRS) and mitochondrial (mLysRS) species of lysyl-tRNA synthetase are encoded by a single gene. Following HIV-1 infection, mLysRS is selectively taken up into viral particles along with the three tRNA isoacceptors. The GagPol polyprotein precursor is involved in this process. Read More

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http://dx.doi.org/10.1016/j.biopen.2016.02.004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5889485PMC

Nitric oxide overcomes Cd and Cu toxicity in grown tobacco plants through increasing contents and activities of rubisco and rubisco activase.

Biochim Open 2016 Jun 2;2:41-51. Epub 2016 Mar 2.

Department of Biological Sciences, Keimyung University, Daegu 42601, South Korea.

Toxic heavy metals such as cadmium (Cd) and copper (Cu) are global problems that are a growing threat to the environment. Despite some heavy metals are required for plant growth and development, others are considered toxic elements and do not play any known physiological role in plant cells. Elevated doses of Cd or Cu cause toxicity in plants and generate damages due to the stress condition and eventually cause a significant reduction in quantity and quality of crop plants. Read More

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http://dx.doi.org/10.1016/j.biopen.2016.02.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5889489PMC
June 2016
1 Read

Screening of endophytic sources of exopolysaccharides: Preliminary characterization of crude exopolysaccharide produced by submerged culture of sp. JF766998 under different cultivation time.

Biochim Open 2016 Jun 2;2:33-40. Epub 2016 Mar 2.

Universidade Estadual de Maringá, Departamento de Biotecnologia, Genética e Biologia Celular, CEP 87020-900, Maringá, Paraná, Brazil.

Endophytic fungi have been described as producers of important bioactive compounds; however, they remain under-exploited as exopolysaccharides (EPS) sources. Therefore, this work reports on EPS production by submerged cultures of eight endophytes isolated from Sw., belonging to genera , , , , and . Read More

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http://dx.doi.org/10.1016/j.biopen.2016.02.003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5889487PMC
June 2016
1 Read

CRISPR/Cas9-mediated target validation of the splicing inhibitor Pladienolide B.

Biochim Open 2016 Dec 24;3:72-75. Epub 2016 Feb 24.

Laboratory for Genome Engineering, Division of Biological Sciences & Center for Desert Agriculture, 4700 King Abdullah University of Science and Technology, Thuwal, 23955-6900, Saudi Arabia.

CRISPR/Cas9 system confers molecular immunity in archeal and bacterial species against invading foreign nucleic acids. CRISPR/Cas9 system is used for genome engineering applications across diverse eukaryotic species. In this study, we demonstrate the utility of the CRISPR/Cas9 genome engineering system for drug target validation in human cells. Read More

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http://dx.doi.org/10.1016/j.biopen.2016.02.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5801905PMC
December 2016
1 Read

A spinach -acetylserine(thiol)lyase homologue, SoCSaseLP, suppresses cysteine biosynthesis catalysed by other enzyme isoforms.

Biochim Open 2016 Jun 8;2:24-32. Epub 2016 Feb 8.

Graduate School of Advanced Integration Science, Chiba University, 1-33 Yayoi-cho, Inage-ku, Chiba 263-8522, Japan.

An enzyme, -acetylserine(thiol)lyase (OASTL), also known as -acetylserine sulfhydrylase or cysteine synthase (CSase), catalyses the incorporation of sulfide into -acetylserine and produces cysteine. We previously identified a cDNA encoding an OASTL-like protein from , (), but a recombinant SoCSaseLP produced in did not show OASTL activity. The exon-intron structure of the gene shared conserved structures with other spinach OASTL genes. Read More

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http://dx.doi.org/10.1016/j.biopen.2016.01.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5889488PMC
June 2016
2 Reads

Assessing Na/H exchange and cell effector functionality in metastatic breast cancer.

Biochim Open 2016 Jun 27;2:16-23. Epub 2016 Jan 27.

Department of Biochemistry, University of Alberta, Edmonton, Alberta, T6G 2H7, Canada.

Metastasis is the leading cause of mortality in patients with breast cancer. In triple-negative breast cancer, high recurrence rates, increased invasive capacity of cells, and their aggressive ability to metastasize at secondary sites dictate patient survival. The Na/H exchanger isoform 1 (NHE1) plays a critical role in controlling the metastatic potential of these cells. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S22140085160000
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http://dx.doi.org/10.1016/j.biopen.2016.01.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5889484PMC
June 2016
1 Read

The peptidyl-prolyl isomerase activity of the wheat cyclophilin, TaCypA-1, is essential for inducing thermotolerance in .

Biochim Open 2016 Jun 30;2:9-15. Epub 2015 Nov 30.

Department of Biotechnology, Guru Nanak Dev University, Amritsar, 143005, Punjab, India.

Growth at high temperatures is one of the desired features for industrial applications of microbes, as it results in decrease in contamination and enhanced solubility of certain substrates. In this study, it is demonstrated that heterologous expression of a wheat cyclophilin, TaCypA-1, confers thermotolerance to . The TaCypA-1 possesses peptidyl-prolyl isomerase (PPIase) activity that catalyses to isomerization of the peptidyl prolyl bonds, a rate limiting step in protein folding. Read More

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http://dx.doi.org/10.1016/j.biopen.2015.11.003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5889472PMC
June 2016
1 Read

Uncovering the role of the flexible C-terminal tail: A model study with Strep-tagged GFP.

Biochim Open 2016 Jun 30;2:1-8. Epub 2015 Nov 30.

Ehime Prefectural Police HQ, Forensic Science Laboratory, Japan.

Recently, it has been recognized that, much like an electric current in an electric circuit, dynamic disruptions from flexible, unstructured regions distal to the active region are transferred through the contact network to the active site and influence protein stability and/or function. As transmembrane proteins frequently possess the β-barrel structure, studies of proteins with this topology are required. The unstructured lid segments of the β-barrel GFP protein are conserved and could play a role in the backbone stabilization required for chromophore function. Read More

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http://dx.doi.org/10.1016/j.biopen.2015.11.004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5889473PMC

Identification of a nicotinamide/nicotinate mononucleotide adenylyltransferase in (GlNMNAT).

Biochim Open 2015 18;1:61-69. Epub 2015 Nov 18.

Department of Chemistry, Universidad Nacional de Colombia, Bogotá Cundinamarca, Colombia.

is an intestinal protozoan parasite that causes giardiasis, a disease of high prevalence in Latin America, Asia and Africa. Giardiasis leads to poor absorption of nutrients, severe electrolyte loss and growth retardation. In addition to its clinical importance, this parasite is of special biological interest due to its basal evolutionary position and simplified metabolism, which has not been studied thoroughly. Read More

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http://dx.doi.org/10.1016/j.biopen.2015.11.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5889475PMC
November 2015
2 Reads

Editorial.

Authors:
Xavier Coumoul

Biochim Open 2015 14;1:60. Epub 2015 Nov 14.

Biochemistry and Toxicology, University Paris Descartes, INSERM UMR-S 1124, Pharmacology, Toxicology and Cell Signaling, France.

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http://dx.doi.org/10.1016/j.biopen.2015.11.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5889471PMC
November 2015
1 Read

Argan oil prevents down-regulation induced by endotoxin on liver fatty acid oxidation and gluconeogenesis and on peroxisome proliferator-activated receptor gamma coactivator-1α, (PGC-1α), peroxisome proliferator-activated receptor α (PPARα) and estrogen related receptor α (ERRα).

Biochim Open 2015 31;1:51-59. Epub 2015 Oct 31.

Univ. Bourgogne-Franche Comté, Laboratoire BioPeroxIL (Biochimie du Peroxysome, Inflammation et Métabolisme Lipidique), EA 7270, 21000 Dijon, France.

In patients with sepsis, liver metabolism and its capacity to provide other organs with energetic substrates are impaired. This and many other pathophysiological changes seen in human patients are reproduced in mice injected with purified endotoxin (lipopolysaccharide, LPS). In the present study, down-regulation of genes involved in hepatic fatty acid oxidation (FAOx) and gluconeogenesis in mice exposed to LPS was challenged by nutritional intervention with Argan oil. Read More

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http://dx.doi.org/10.1016/j.biopen.2015.10.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5889474PMC
October 2015
3 Reads

Method for estimating protein binding capacity of polymeric systems.

Biochim Open 2015 24;1:40-50. Epub 2015 Oct 24.

RAFT Institute of Plastic Surgery, Mount Vernon Hospital, Northwood, HA6 2RN, UK.

Composite biomaterials made from synthetic and protein-based polymers are extensively researched in tissue engineering. To successfully fabricate a protein-polymer composite, it is critical to understand how strongly the protein binds to the synthetic polymer, which occurs through protein adsorption. Currently, there is no cost-effective and simple method for characterizing this interfacial binding. Read More

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http://dx.doi.org/10.1016/j.biopen.2015.10.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5889478PMC
October 2015
1 Read

Proline substitutions in a Mip-like peptidyl-prolyl isomerase severely affect its structure, stability, shape and activity.

Biochim Open 2015 23;1:28-39. Epub 2015 Jul 23.

Department of Biochemistry, Bose Institute, P-1/12, CIT Scheme VII M, Kolkata 700054, West Bengal, India.

FKBP22, an -specific peptidyl-prolyl - isomerase, shows substantial homology with the Mip-like virulence factors. Mip-like proteins are homodimeric and possess a V-shaped conformation. Their N-terminal domains form dimers, whereas their C-terminal domains bind protein/peptide substrates and distinct inhibitors such as rapamycin and FK506. Read More

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http://dx.doi.org/10.1016/j.biopen.2015.07.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5889476PMC

Palmitoyl-ceramide accumulation with necrotic cell death in A549 cells, followed by a steep increase in sphinganine content.

Authors:
Mototeru Yamane

Biochim Open 2015 21;1:11-27. Epub 2015 Jun 21.

Department of Biochemistry, Tokyo Medical University, 6-1-1 Shinjuku, Shinjuku-ku, Tokyo 160-0023, Japan.

Ceramides (Cers) have recently been identified as key signaling molecules that mediate biological functions such as cell growth, differentiation, senescence, apoptosis, and autophagy. However, the functions of Cer accumulation in necrotic cell death remain unknown. The aim of this study was to clarify the relationship between Cer accumulation with inhibition of the conversion pathway of Cer and concomitant necrotic cell death. Read More

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http://dx.doi.org/10.1016/j.biopen.2015.06.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5889477PMC