8,210 results match your criteria Biochimie[Journal]


Metabolic and physiological perturbations of Escherichia coli W3100 by bacterial small RNA RyhB.

Biochimie 2019 Apr 16. Epub 2019 Apr 16.

Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, 230027, People's Republic of China. Electronic address:

RyhB is a key regulator of iron level in Escherichia coli (E. coli), which assists in conserving iron for life-sustaining cellular functions when cytoplasmic levels of the ferrous form of iron is limited. RyhB affects glucose metabolism. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S03009084193013
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http://dx.doi.org/10.1016/j.biochi.2019.04.016DOI Listing
April 2019
1 Read

TLR2 and TLR4 mediate an activation of adipose tissue renin-angiotensin system induced by uric acid.

Biochimie 2019 Apr 16. Epub 2019 Apr 16.

Department of Endocrinology, Central War Zone General Hospital of the Chinese People's Liberation Army, Wuhan, China.

Both hyperuricemia and adipose tissue renin-angiotensin system (RAS) are closely associated with multiple metabolic and cardiovascular diseases. We previously reported that uric acid could upregulate tissue RAS in adipocytes. In the present study, we aimed to reveal the involvement of toll-like receptors (TLRs) in uric acid-induced RAS activation in adipose tissue. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S03009084193012
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http://dx.doi.org/10.1016/j.biochi.2019.04.013DOI Listing
April 2019
1 Read

Up-regulation of microRNA-496 suppresses proliferation, invasion, migration and in vivo tumorigenicity of human osteosarcoma cells by targeting eIF4E.

Biochimie 2019 Apr 15. Epub 2019 Apr 15.

The Second Department of Orthopedics, The Fifth Hospital of Harbin, Harbin, 150040, Heilongjiang Province, PR China. Electronic address:

Osteosarcoma is an aggressive bone tumor characterized by a high level of genetic instability and recurring DNA deletions and amplifications. This study aims to investigate how microRNA-496 (miR-496) affects proliferation, invasion, and migration of human osteosarcoma (OS) cells and in vivo tumorigenicity by targeting eukaryotic translation initiation factor 4E (eIF4E). Microarray-based gene expression profiling involving OS was used in order to identify differentially expressed genes. Read More

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http://dx.doi.org/10.1016/j.biochi.2019.04.017DOI Listing

CREPT promotes glioma cell proliferation and invasion by activating Wnt/β-catenin pathway and is a novel target of microRNA-596.

Biochimie 2019 Apr 14. Epub 2019 Apr 14.

Department of Neurosurgery, Tangdu Hospital, Fourth Military Medical University, Xi'an, 710038, Shaanxi Province, China. Electronic address:

Cell cycle-related and expression elevated protein in tumor (CREPT) is emerging as a novel cancer-related gene that is dysregulated in many kinds of malignancies. However, the expression and biological role of CREPT in glioma remains unclear. In the present study, we aimed to explore the potential function and regulation mechanism of CREPT in glioma. Read More

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http://dx.doi.org/10.1016/j.biochi.2019.04.014DOI Listing

Physiological roles of antisense RNAs in prokaryotes.

Biochimie 2019 Apr 14. Epub 2019 Apr 14.

CNRS UMR8261, IBPC, 13 Rue Pierre et Marie Curie, 75005, Paris, France. Electronic address:

Prokaryotes encounter constant and often brutal modifications to their environment. In order to survive, they need to maintain fitness, which includes adapting their protein expression patterns. Many factors control gene expression but this review focuses on just one, namely antisense RNAs (asRNAs), a class of non-coding RNAs (ncRNAs) characterized by their location in cis and their perfect complementarity with their targets. Read More

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http://dx.doi.org/10.1016/j.biochi.2019.04.015DOI Listing

70 years of serotonin.

Biochimie 2019 Apr 12. Epub 2019 Apr 12.

Institut national de la recherche scientifique (INRS), Centre Armand Frappier Santé Biotechnologie, 531 boulevard des Prairies, Laval, Québec, H7V 1B7, Canada. Electronic address:

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http://dx.doi.org/10.1016/j.biochi.2019.04.012DOI Listing

Crataeva tapia bark lectin (CrataBL) is a chemoattractant for endothelial cells that targets heparan sulfate and promotes in vitro angiogenesis.

Biochimie 2019 Apr 11. Epub 2019 Apr 11.

Department of Biochemistry Federal University of São Paulo (UNIFESP), Brazil. Electronic address:

Formation of new blood vessels from preexisting ones, a process known as angiogenesis, is one of the limiting steps for success in treatment of ischemic disorders. Therefore, efforts to understanding and characterize new agents capable to stimulate neovascularization are a worldwide need. Crataeva tapia bark lectin (CrataBL) has been shown to have chemoattractant properties for endothelial cells through the stimulation of migration and invasiveness of human umbilical vein endothelial cells (HUVEC) because it is a positively charged protein with high affinity to glycosaminoglycan. Read More

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http://dx.doi.org/10.1016/j.biochi.2019.04.011DOI Listing

GLUT1 is associated with sphingolipid-organized, cholesterol-independent domains in L929 mouse fibroblast cells.

Biochimie 2019 Apr 11;162:88-96. Epub 2019 Apr 11.

Department of Chemistry and Biochemistry, Calvin College, Grand Rapids, MI, 49546, USA. Electronic address:

Glucose is a preferred metabolite in most mammalian cells, and proper regulation of uptake is critical for organism homeostasis. The glucose transporter 1 (GLUT1) is responsible for glucose uptake in a wide variety of cells and appears to be regulated in a tissue specific manner. Therefore, a better understanding of GLUT1 regulation within its various cellular environments is essential for developing therapeutic strategies to treat disorders associated with glucose homeostasis. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S03009084193011
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http://dx.doi.org/10.1016/j.biochi.2019.04.010DOI Listing
April 2019
2 Reads

Identification of a peptide derived from a Bothrops moojeni metalloprotease with in vitro inhibitory action on the Plasmodium falciparum purine nucleoside phosphorylase enzyme (PfPNP).

Biochimie 2019 Apr 9;162:97-106. Epub 2019 Apr 9.

Center for the Study of Biomolecules Applied to Health, CEBio, Oswaldo Cruz Foundation Rondônia, FIOCRUZ, Porto Velho, Rondônia, Brazil; Graduate Program in Experimental Biology, PGBIOEXP, Federal University of Rondônia, UNIR, Porto Velho, Rondônia, Brazil; National Institute of Science and Technology in Epidemiology of the Western Amazonia, INCT-EpiAmO, Porto Velho, Rondônia, Brazil; São Lucas University Center, UniSL, Porto Velho, Rondônia, Brazil.

There is a growing need for research on new antimalarial agents against Plasmodium falciparum infection, especially in regards to planning molecular architecture for specific molecular targets of the parasite. Thus, a metalloprotease from Bothrops moojeni, known as BmooMPα-I, was explored in this study, through in silico assays, aiming at the development of a peptide generated from this molecule with potential inhibitory action on PfPNP, an enzyme necessary for the survival of the parasite. In order to isolate BmooMPα-I, cation exchange and reverse phase chromatographies were performed, followed by in vitro assays of antiparasitic activity against the W2 strain of P. Read More

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http://dx.doi.org/10.1016/j.biochi.2019.04.009DOI Listing
April 2019
2 Reads

In vivo assembly of eukaryotic signal recognition particle: A still enigmatic process involving the SMN complex.

Biochimie 2019 Apr 9. Epub 2019 Apr 9.

Ingénierie Moléculaire et Physiopathologie Articulaire, UMR 7365 CNRS-University of Lorraine, Biopôle de l'Université de Lorraine, Campus Brabois-Santé, 9 avenue de la forêt de Haye, BP 20199, 54505 Vandoeuvre-les-Nancy, France. Electronic address:

The signal recognition particle (SRP) is a universally conserved non-coding ribonucleoprotein complex that is essential for targeting transmembrane and secretory proteins to the endoplasmic reticulum. Its composition and size varied during evolution. In mammals, SRP contains one RNA molecule, 7SL RNA, and six proteins: SRP9, 14, 19, 54, 68 and 72. Read More

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http://dx.doi.org/10.1016/j.biochi.2019.04.007DOI Listing

Kinetics of inactivation of staphylolytic enzymes: Qualitative and quantitative description.

Biochimie 2019 Apr 6;162:77-87. Epub 2019 Apr 6.

Department of Chemical Enzymology, Faculty of Chemistry, M.V. Lomonosov Moscow State University, Moscow, Russia; Division of Molecular Pharmaceutics, Center for Nanotechnology in Drug Delivery, UNC Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, USA.

Lysin 2638aR and chimeric Ply187AN-KSH3b fusion protein are capable of lysing antibiotic-resistant strains of Staphylococcus aureus and are promising alternatives to antibiotics. Studies on the stability and structure of lysins 2638aR and Ply187AN-KSH3b are important for assessing the feasibility of their practical use. Both lysins are highly active at physiological pH (7. Read More

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http://dx.doi.org/10.1016/j.biochi.2019.04.005DOI Listing

The homeoprotein Msx1 cooperates with Pkn1 to prevent terminal differentiation in myogenic precursor cells.

Biochimie 2019 Apr 6;162:55-65. Epub 2019 Apr 6.

State Key Laboratory of Genetic Engineering and Collaborative Innovation Center of Genetics and Development, School of Life Sciences and Zhongshan Hospital, Fudan University, Shanghai, 200438, PR China. Electronic address:

The homeoprotein Msx1 plays a critical role in embryonic patterning, particularly to maintain myogenic progenitor cell fate. However, the mechanisms underlying Msx1-mediated inhibition of myogenesis remain largely unknown. Here, we show that Msx1 cooperates with the protein kinase C-related kinase 1 (Pkn1), a member of the protein kinase C-related kinase family, to prevent the terminal differentiation of myogenic precursor cells. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S03009084193010
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http://dx.doi.org/10.1016/j.biochi.2019.04.003DOI Listing
April 2019
8 Reads

Suppression in advanced glycation adducts of human serum albumin by bio-enzymatically synthesized gold and silver nanoformulations: A potential tool to counteract hyperglycemic condition.

Biochimie 2019 Apr 6;162:66-76. Epub 2019 Apr 6.

Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, 202002, UP, India. Electronic address:

Advanced glycation end-products (AGEs) from non-enzymatic glycation are implicated in several degenerative diseases, including being a crucial contributor in secondary complications of diabetes. This has garnered significant scientific interest in inhibiting agents of AGEs to prevent and remediate disorders arising due to glycation. In the current study, inhibitory effects on AGEs formation were investigated using bio-enzymatically synthesized nanoformulations of gold (AuNPs) and silver (AgNPs) by physiologically important enzyme, β galactosidase. Read More

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http://dx.doi.org/10.1016/j.biochi.2019.04.004DOI Listing
April 2019
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TEMPORARY REMOVAL: The apoptosis and GLP-1 hyposecretion induced by LPS via RIP/ROS/mTOR pathway in GLUTag cells.

Biochimie 2019 Apr 4. Epub 2019 Apr 4.

Division of Endocrinology, Department of Internal Medicine, The First Affiliated Hospital of Zhengzhou University, 450052, Zhengzhou, China.

The publisher regrets that this article has been temporarily removed. A replacement will appear as soon as possible in which the reason for the removal of the article will be specified, or the article will be reinstated. The full Elsevier Policy on Article Withdrawal can be found at https://www. Read More

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http://dx.doi.org/10.1016/j.biochi.2019.04.001DOI Listing
April 2019
2 Reads

Serotonin and orthodontic tooth movement.

Biochimie 2019 Apr 3. Epub 2019 Apr 3.

Institut Imagine, INSERM U1163, CNRS ERL, 8254, Hôpital Necker, Laboratoire d'excellence GR-Ex, Paris, France; Département d'Hématologie, Gustave Roussy Cancer Campus Grand Paris, Villejuif, France. Electronic address:

Peripheral serotonin continuously reveals its unexpected involvements in many organ functions. In bone tissue, there is an increasing evidence for a local serotonergic system affecting the cellular and molecular actors involved in bone turnover. During orthodontic treatment, tooth movement relies on bone remodeling, itself a result of the inflammatory process triggered by application of orthodontic forces to the teeth. Read More

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http://dx.doi.org/10.1016/j.biochi.2019.04.002DOI Listing
April 2019
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Serotonin-estrogen interactions: What can we learn from pregnancy?

Biochimie 2019 Apr 1. Epub 2019 Apr 1.

INRS-Institut Armand-Frappier, 531, boulevard des Prairies, Laval, QC, H7V 1B7, Canada; Center for Interdisciplinary Research on Well-Being, Health, Society and Environment (Cinbiose), Université du Québec à Montréal, C.P.8888, succ. Centre-Ville, Montréal, QC, H3C 3P8, Canada. Electronic address:

We have reviewed the scientific literature related to four diseases in which to serotonin (5-HT) is involved in the etiology, herein named 5-HT-linked diseases, and whose prevalence is influenced by estrogenic status: depression, migraine, irritable bowel syndrome and eating disorders. These diseases all have in common a sex-dimorphic prevalence, with women more frequently affected than men. The co-occurrence between these 5-HT-linked diseases suggests that they have common physiopathological mechanisms. Read More

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http://dx.doi.org/10.1016/j.biochi.2019.03.023DOI Listing

Repurposing of auranofin: Thioredoxin reductase remains a primary target of the drug.

Biochimie 2019 Apr 1;162:46-54. Epub 2019 Apr 1.

Department of Oncology-Pathology, Karolinska Institutet, SE-171 76, Stockholm, Sweden; Department of Medical and Health Sciences, Linköping University, SE-581 83, Linköping, Sweden. Electronic address:

Auranofin is a gold (I)-containing compound used for the treatment of rheumatic arthritis. Auranofin has anticancer activity in animal models and is approved for clinical trials for lung and ovarian carcinomas. Both the cytosolic and mitochondrial forms of the selenoprotein thioredoxin reductase (TrxR) are well documented targets of auranofin. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S03009084193008
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http://dx.doi.org/10.1016/j.biochi.2019.03.015DOI Listing
April 2019
7 Reads

Biochemical and molecular characterization of the hyaluronidase from Bothrops atrox Peruvian snake venom.

Biochimie 2019 Apr 1;162:33-45. Epub 2019 Apr 1.

Research and Development Center, Ezequiel Dias Foundation, 30510-010, Belo Horizonte, MG, Brazil.

Snake venoms are a rich source of enzymes such as metalloproteinases, serine proteinases phospholipases A2 and myotoxins, that have been well characterized structurally and functionally. However, hyaluronidases (E.C. Read More

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http://dx.doi.org/10.1016/j.biochi.2019.03.022DOI Listing
April 2019
2 Reads

An ancient mechanism of arginine-specific substrate cleavage: What's 'up' with NSP4?

Biochimie 2019 Apr 1. Epub 2019 Apr 1.

Department of Early Discovery Biochemistry, Genentech Inc, 1 DNA Way, South San Francisco, CA 94080, USA. Electronic address:

The recently discovered neutrophil serine protease 4 (NSP4) is the fourth member of the NSP family, which includes the well-studied neutrophil elastase, proteinase 3 and cathepsin G. Like the other three NSP members, NSP4 is synthesized by myeloid precursors in the bone marrow and, after cleavage of the two-amino acid activation peptide, is stored as an active protease in azurophil granules of neutrophils. Based on its primary amino acid sequence, NSP4 is predicted to have a shallow S1 specificity pocket with elastase-like substrate specificity. Read More

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http://dx.doi.org/10.1016/j.biochi.2019.03.020DOI Listing
April 2019
1 Read
2.963 Impact Factor

Design of cross-linked RNA/protein complexes for structural studies.

Biochimie 2019 Mar 30. Epub 2019 Mar 30.

Laboratoire de Cristallographie et RMN Biologiques, CNRS, Université Paris Descartes, Sorbonne Paris Cité, Paris, France; Laboratoire d'Expression Génétique Microbienne, CNRS, Université Paris Diderot, Sorbonne Paris Cité, Institut de Biologie Physico-Chimique, IBPC, Paris, France. Electronic address:

Crystallographic studies of RNA/protein complexes are primordial for the understanding of recognition determinants and catalytic mechanisms in the case of enzymes. However, due to the flexibility and propensity to conformational heterogeneity of RNAs, as well as the mostly electrostatic interactions of RNA/protein complexes, they are difficult to crystallize. We present here a method to trap the two interacting partners in a covalent complex, based on a modified reactive RNA allowing the use of the full range of common crystallogenesis tools. Read More

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http://dx.doi.org/10.1016/j.biochi.2019.03.021DOI Listing

SOX11 hypermethylation as a tumor biomarker in endometrial cancer.

Biochimie 2019 Mar 29;162:8-14. Epub 2019 Mar 29.

Department of Obstetrics and Gynecology, Medical College of Wisconsin, Milwaukee, WI, 53226, USA. Electronic address:

We previously reported that SOX4 is overexpressed in endometrial cancer and that it partially contributes to hypermethylation of miR-129-2 and miR-203. The current study seeks to identify methylation and expression levels of the SOX gene family in endometrial carcinomas. Methylation levels of the 16 SOX gene family members were measured by combining bisulfite restriction analysis (COBRA), MassARRAY, and pyrosequencing assays of cell lines and endometrial cancer samples. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S03009084193008
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http://dx.doi.org/10.1016/j.biochi.2019.03.019DOI Listing
March 2019
5 Reads

Sex-specific polymorphism of MET1 and ARR17 genes in Populus × sibirica.

Biochimie 2019 Mar 29;162:26-32. Epub 2019 Mar 29.

Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilova 32, Moscow, 119991, Russia. Electronic address:

The genus Populus is an effective model in tree genetics. This genus includes dioecious species and, recently, whole genome resequencing of P. trichocarpa and P. Read More

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http://dx.doi.org/10.1016/j.biochi.2019.03.018DOI Listing

Structural insights into the 3'-end mRNA maturation machinery: Snapshot on polyadenylation signal recognition.

Biochimie 2019 Mar 28. Epub 2019 Mar 28.

INSERM U1212 - CNRS 5320 & Université de Bordeaux, 146 rue Léo Saignat, 33000, Bordeaux, France. Electronic address:

Pre-mRNA 3'-end maturation is achieved by a mechanism requiring four different protein complexes assembled from approximately twenty factors. A global understanding of this essential process is still missing due to the inability to structurally characterize the entire complexes, even though structures of the isolated factors have been obtained. In this review, we summarize recent findings regarding the atomic description of one of the major players, the Cleavage and Polyadenylation Specificity Factor complex (CPSF in human, CPF in yeast). Read More

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http://dx.doi.org/10.1016/j.biochi.2019.03.016DOI Listing
March 2019
1 Read
2.963 Impact Factor

Dual cellular localization of the Leishmania amazonensis Rbp38 (LaRbp38) explains its affinity for telomeric and mitochondrial DNA.

Biochimie 2019 Mar 28;162:15-25. Epub 2019 Mar 28.

Department of Genetics, Biosciences Institute, São Paulo State University (UNESP), Botucatu, SP, Brazil. Electronic address:

Rbp38 is a protein exclusively found in trypanosomatid parasites, including Leishmania amazonensis, the etiologic agent of tegumentar leishmaniasis in the Americas. The protein was first described as a Leishmania tarentolae mitochondrial RNA binding protein. Later, it was shown that the trypanosomes Rbp38 orthologues were exclusively found in the mitochondria and involved in the stabilization and replication of kinetoplast DNA (kDNA). Read More

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http://dx.doi.org/10.1016/j.biochi.2019.03.017DOI Listing

Differential micro-RNA expression in diabetic patients with abdominal aortic aneurysm.

Biochimie 2019 Mar 26;162:1-7. Epub 2019 Mar 26.

Université Côte d'Azur, CHU, Inserm U1065, C3M, Nice, France; Division of Cardiovascular Medicine, Department of Medicine, University of Cambridge, Cambridge, CB20 SZ, UK; Institut National de la Santé et de la Recherche Médicale (Inserm), Unit 970, Paris Cardiovascular Research Center, 75015, Paris, France; Department of Clinical Biochemistry, University Hospital of Nice, France. Electronic address:

Objectives: The potential implication of micro-RNAs (miRs) in the negative association between diabetes and abdominal aortic aneurysm (AAA) has so far never been addressed. The aim of this study was to compare miR expression between diabetic and non-diabetic patients with AAA.

Methods: Ten diabetic patients were prospectively included and compared to 10 age- and sex-matched non-diabetic patients with infrarenal AAA. Read More

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http://dx.doi.org/10.1016/j.biochi.2019.03.012DOI Listing
March 2019
1 Read

Imaging of extracellular cathepsin S activity by a selective near infrared fluorescence substrate-based probe.

Biochimie 2019 Mar 23. Epub 2019 Mar 23.

Université de Tours, Tours, France; INSERM, UMR 1100, Research Center for Respiratory Diseases (CEPR), Team: "Proteolytic Mechanisms in Inflammation", Tours, France. Electronic address:

We designed a near-infrared fluorescent substrate-based probe (SBP), termed MG101, for monitoring extracellular cathepsin S (CatS) activity. We conceived a fused peptide hairpin loop-structure, combining a CatS recognition domain, an electrostatic zipper (with complementary charges of a polyanionic (D-Glu) segment and a polycationic (D-Arg) motif, as well as a N and C terminal Förster resonance energy transfer pair (donor: AlexaFluor680; quencher: BHQ3) to facilitate activity-dependent imaging. MG101 showed excellent stability since no fluorescence release corresponding to a self-dequenching was observed in the presence of either 2 M NaCl or after incubation at a broad range of pH (2. Read More

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http://dx.doi.org/10.1016/j.biochi.2019.03.013DOI Listing

The yeast C/D box snoRNA U14 adopts a "weak" K-turn like conformation recognized by the Snu13 core protein in solution.

Biochimie 2019 Mar 23. Epub 2019 Mar 23.

Structure and Dynamics of Biomolecular Machines, Université de Strasbourg, CNRS, Architecture & Réactivité de l'ARN, UPR 9002, Institut de Biologie Moléculaire et Cellulaire (IBMC), 15 rue René Descartes, F-67000, Strasbourg, France. Electronic address:

Non-coding RNAs associate with proteins to form ribonucleoproteins (RNPs), such as ribosome, box C/D snoRNPs, H/ACA snoRNPs, ribonuclease P, telomerase and spliceosome to ensure cell viability. The assembly of these RNA-protein complexes relies on the ability of the RNA to adopt the correct bound conformation. K-turn motifs represent ubiquitous binding platform for proteins found in several cellular environment. Read More

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http://dx.doi.org/10.1016/j.biochi.2019.03.014DOI Listing

HIV-1 gRNA, a biological substrate, uncovers the potency of DDX3X biochemical activity.

Biochimie 2019 Mar 22. Epub 2019 Mar 22.

CiTCOM, Université Paris Descartes, CNRS UMR 8038, Paris, France. Electronic address:

DEAD-box helicases play central roles in the metabolism of many RNAs and ribonucleoproteins by assisting their synthesis, folding, function and even their degradation or disassembly. They have been implicated in various phenomena, and it is often difficult to rationalize their molecular roles from in vivo studies. Once purified in vitro, most of them only exhibit a marginal activity and poor specificity. Read More

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http://dx.doi.org/10.1016/j.biochi.2019.03.008DOI Listing
March 2019
2 Reads

Heavy metal hypertolerant eukaryotic aldehyde dehydrogenase isolated from metal contaminated soil by metatranscriptomics approach.

Biochimie 2019 May 21;160:183-192. Epub 2019 Mar 21.

Department of Biotechnology, Thapar Institute of Engineering & Technology, Patiala, Punjab, 147004, India. Electronic address:

Constant addition of heavy metal pollutants in soil resulting from anthropogenic activities can prove detrimental to both macro and micro life forms inhabiting the ecosystem. The potential functional roles of eukaryotic microbes in such environment were explored in this study by metatranscriptomics approach. Sized eukaryotic cDNA libraries, library A (<0. Read More

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http://dx.doi.org/10.1016/j.biochi.2019.03.010DOI Listing

MicroRNA-34 suppresses proliferation of human ovarian cancer cells by triggering autophagy and apoptosis and inhibits cell invasion by targeting Notch 1.

Biochimie 2019 May 21;160:193-199. Epub 2019 Mar 21.

Department of Gynecology and Obstetrics, The Second Hospital of Jilin University, Changchun, Jilin, 130041, China. Electronic address:

Ovarian cancer is one the prevalent cancers in women and is responsible for 5% of all the cancer related mortalities in women. Owing to late diagnosis, frequent relapses, side effects of chemotherapy, development of drug resistance, there is pressing need to screen out novel and effective treatment options. Accumulating evidences suggest that miRNAs may prove essential therapeutic targets for the treatment of cancer. Read More

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http://dx.doi.org/10.1016/j.biochi.2019.03.011DOI Listing
May 2019
1 Read

Highly active extracellular α-class carbonic anhydrase of Cyanothece sp. ATCC 51142.

Biochimie 2019 May 18;160:200-209. Epub 2019 Mar 18.

К.А. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya street 35, Moscow, 127276, Russia.

Here, for the first time, we report the presence of highly active extracellular carbonic anhydrase (CA) of α-class in cyanobacterial cells. The enzyme activity was confirmed both in vivo in intact cells and in vitro, using the recombinant protein. CA activity in intact cells of Cyanothece sp. Read More

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http://dx.doi.org/10.1016/j.biochi.2019.03.009DOI Listing

Periostin contributes to renal and cardiac dysfunction in rats with chronic kidney disease: Reduction of PPARα.

Biochimie 2019 May 16;160:172-182. Epub 2019 Mar 16.

Department of Nephrology, Shengjing Hospital of China Medical University, Shenyang, 110004, People's Republic of China. Electronic address:

POSTN knockdown inhibits the formation of NLRP3 inflammasome in rat myocardium.Periostin (POSTN), an extracellular matrix protein, and peroxisome proliferator-activated receptor alpha (PPARα), a ligand-activated nuclear transcription factor, are reported to be involved in renal and cardiac dysfunction associated with chronic kidney disease (CKD), respectively. This study is performed to investigate how POSTN-PPARα axis affects the progress of CKD. Read More

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http://dx.doi.org/10.1016/j.biochi.2019.03.003DOI Listing
May 2019
1 Read

Primers on nutrigenetics and nutri(epi)genomics: Origins and development of precision nutrition.

Biochimie 2019 May 13;160:156-171. Epub 2019 Mar 13.

Unit of Molecular Biology, School of Pharmacy, University of Camerino, 62032, Camerino, MC, Italy. Electronic address:

Understanding the relationship between genotype and phenotype is a central goal not just for genetics but also for medicine and biological sciences. Despite outstanding technological progresses, genetics alone is not able to completely explain phenotypes, in particular for complex diseases. Given the existence of a "missing heritability", growing attention has been given to non-mendelian mechanisms of inheritance and to the role of the environment. Read More

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http://dx.doi.org/10.1016/j.biochi.2019.03.006DOI Listing

Characterization of the nicotinamide adenine dinucleotides (NAD and NADP) binding sites of the monomeric isocitrate dehydrogenases from Campylobacter species.

Biochimie 2019 May 12;160:148-155. Epub 2019 Mar 12.

Institute of Molecular Biology and Biotechnology and the Research Center of Life Omics and Health, College of Life Sciences, Anhui Normal University, Wuhu, 241000, Anhui, China. Electronic address:

Monomeric isocitrate dehydrogenases (IDHs) have once been proposed to be exclusively NADP-specific. Intriguingly, we recently have reported an NAD-specific monomeric IDH from Campylobacter sp. FOBRC14 (CaIDH). Read More

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http://dx.doi.org/10.1016/j.biochi.2019.03.007DOI Listing
May 2019
2.963 Impact Factor

Cyanidin-3-glucoside induces mesenchymal to epithelial transition via activating Sirt1 expression in triple negative breast cancer cells.

Biochimie 2019 Mar 12;162:107-115. Epub 2019 Mar 12.

Department of Clinical Nutrition, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200080, China. Electronic address:

Triple-negative breast cancer (TNBC) is a heterogeneous group of breast cancer with one common feature: distinctly metastatic nature with higher rate of relapse and shorter survival compared with other subtypes of breast cancer. The epithelial to mesenchymal transition (EMT) is highly associated with cancer metastasis. Cyanidin-3-glucoside (C3G), the most abundant anthocyanin pigment enriched in fresh fruits and vegetables, showed ideal anti-oxidant property. Read More

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http://dx.doi.org/10.1016/j.biochi.2019.03.004DOI Listing
March 2019
1 Read

Proteinase 3 phosphonic inhibitors.

Biochimie 2019 Mar 13. Epub 2019 Mar 13.

Wroclaw University of Science and Technology, Faculty of Chemistry, Division of Medicinal Chemistry and Microbiology, Wybrzeże Wyspiańskiego 27, 50-370, Wrocław, Poland. Electronic address:

Neutrophils are one of the most important military services of the armed forces of the immune system, a crucial line of defense against bacterial or fungal onslaughts. One of their mechanisms of action relies on the production of serine proteases. One of these enzymes is proteinase 3 (PR3), which is engaged in the processing of pro-inflammatory cytokines, receptors, heat shock proteins and in the generation of antibacterial peptides. Read More

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http://dx.doi.org/10.1016/j.biochi.2019.03.005DOI Listing
March 2019
2.963 Impact Factor

Impact of proteolysis on cancer stem cell functions.

Biochimie 2019 Mar 12. Epub 2019 Mar 12.

Institute of Molecular Medicine and Cell Research, Medical Faculty, University of Freiburg, Freiburg, Germany; BIOSS Centre for Biological Signaling Studies, Freiburg, Germany; German Cancer Consortium (DKTK) Partner Site Freiburg, German Cancer Research Center (DKFZ), Heidelberg, Germany; Comprehensive Cancer Center Freiburg, Freiburg, Germany. Electronic address:

Cancer cells within a tumor are heterogeneous and exist in a variety of functionally distinct cell states, which are thought to be hierarchically organized. The cell on top of this hierarchy, the cancer stem cell (CSC) or, alternatively, tumor initiating cell (TIC), is responsible for initiation, maintenance, progression, and relapse of tumors. For the execution of these functions, CSC are equipped with distinct molecular tools. Read More

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http://dx.doi.org/10.1016/j.biochi.2019.03.002DOI Listing
March 2019
2 Reads

Erratum to 'Folate and choline absorption and uptake: Their role in fetal development' [Biochimie 158 (2019) 10-19].

Biochimie 2019 05 12;160:129. Epub 2019 Mar 12.

Institute of Human Nutrition and Dietetics, Poznań University of Life Sciences, Poland. Electronic address:

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http://dx.doi.org/10.1016/j.biochi.2019.03.001DOI Listing

LC-MSMS characterisations of scymnol and oxoscymnol biotransformations in incubation mixtures of rat liver microsomes.

Biochimie 2019 May 4;160:130-140. Epub 2019 Mar 4.

Natural Products Research Group, School of Health & Biomedical Sciences, RMIT University, Bundoora, Victoria, 3083, Australia.

The bile alcohol 5β-scymnol ([24R]-(+)-5β-cholestan-3α,7α,12α,24,26,27-hexol) is a therapeutic nutraceutical derived from marine sources, however very little is known about its potential for biotransformation as a xenobiotic in higher vertebrates. In this study, biotransformation products of scymnol catalysed by liver microsomes isolated from normal and streptozotocin (STZ)-treated male Wistar rats were characterised by liquid chromatography-tandem mass spectroscopy (LC-MSMS). In order of increasing polarity relative to the reversed phase sorbent, structural assignments were made for four biotransformation products, namely 3-oxoscymnol (5β-cholestan-3-one-7α,12α,24,26,27-pentol); 7-oxoscymnol (5β-cholestan-7-one-3α,12α,24,26,27-pentol); 3β-scymnol (5β-cholestan-3β,7α,12α,24,26,27-hexol) and 6β-hydroxyscymnol (5β-cholestan-3α,6β,7α,12α,24,26,27-heptol). Read More

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http://dx.doi.org/10.1016/j.biochi.2019.02.016DOI Listing

Conformational features of intramolecular G4-DNA constrained by single-nucleotide loops.

Biochimie 2019 May 3;160:122-128. Epub 2019 Mar 3.

Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991, Moscow, Russia. Electronic address:

Conformation of the telomeric DNA fragment dG(TTAG) depends on multiple factors including solution conditions, length, and the nucleotide sequence of the flanking regions. In potassium solution, this sequence tends to adopt hybrid (3 + 1) G-quadruplex (G4) Form 1 or Form 2 conformation contingent on the flanking nucleotides. Theoretically, other (3 + 1) G4 folds (beyond Forms 1 and 2) are not sterically forbidden, but are presumably energetically disfavored. Read More

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http://dx.doi.org/10.1016/j.biochi.2019.02.013DOI Listing
May 2019
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Grem2 mediates podocyte apoptosis in high glucose milieu.

Biochimie 2019 May 1;160:113-121. Epub 2019 Mar 1.

Feinstein Institute for Medical Research and Donald and Barbara Zucker School of Medicine at Hofstra/Northwell, Manhasset, NY, United States. Electronic address:

Background: Increased DAN protein (Grem1, Grem2, Grem3, Cerberus, NBL1, SOST, and USAG1) levels are often associated with severe disease-states in adult kidneys. Grem1, SOST, and USAG1 have been demonstrated to be upregulated and play a critical role in the progression of diabetic nephropathy (DN); however, the expression and the role of other DAN family members in DN have not been reported yet. In this study, we investigated the expression and the role of Grem2 in the development of renal lesions in mice with type 2 DN. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S03009084193006
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http://dx.doi.org/10.1016/j.biochi.2019.02.015DOI Listing
May 2019
6 Reads
2.963 Impact Factor

A novel fluorescence based assay for the detection of organophosphorus pesticide exposed cholinesterase activity using 1-naphthyl acetate.

Biochimie 2019 May 26;160:100-112. Epub 2019 Feb 26.

Department of Experimental Medicine and Biotechnology, Postgraduate Institute Institute of Medical Education and Research, Chandigarh, 160012, India. Electronic address:

Acetylcholinesterase (AChE) is the primary target of organophosphorus pesticides (OPs). Ellman's method using Acetylthiocholine (ATCh) is the standard approach for the detection of AChE activity. Though ATCh is a popular substrate, it has certain drawbacks as well. Read More

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http://dx.doi.org/10.1016/j.biochi.2019.02.014DOI Listing
May 2019
2 Reads

Nybomycin-producing Streptomyces isolated from carpenter ant Camponotus vagus.

Biochimie 2019 May 21;160:93-99. Epub 2019 Feb 21.

Skolkovo Institute of Science and Technology, Skolkovo, Moscow Region, 143025, Russia; Department of Chemistry and A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, 119992, Russia. Electronic address:

A novel strain of Actinomycetes was isolated from the body of an ant (Camponotus vagus Scopoli) and its genetic and morphological properties were characterized. The 16S rDNA gene sequence analysis of the isolate revealed its high phylogenetic relationship with type strains of Streptomyces violaceochromogenes NBRC 13100. As a result of antimicrobial activity assessment, it was found that the fermentation broth of the isolated strain both inhibited the growth and induced the SOS response in E. Read More

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http://dx.doi.org/10.1016/j.biochi.2019.02.010DOI Listing
May 2019
1 Read

Fatty acids and lipopolysaccharides from health to disease.

Biochimie 2019 Apr 22;159:1-2. Epub 2019 Feb 22.

UMR Lipides/Nutrition/Cancer U1231 INSERM/Univ Bourgogne-Franche Comté/AgroSupDijon, AgroSup Dijon, Team NUTox, 1, Esplanade Erasme, 21000, Dijon, France. Electronic address:

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http://dx.doi.org/10.1016/j.biochi.2019.02.008DOI Listing

Strategies in the design of endosomolytic agents for facilitating endosomal escape in nanoparticles.

Biochimie 2019 May 21;160:61-75. Epub 2019 Feb 21.

Research and Scientific Studies Unit, College of Nursing and Allied Health Sciences, Jazan University, Jazan, 45142, Saudi Arabia.

Nanoparticles (NPs) are one of the leading and promising technologies for gene and drug delivery. However, despite continuous advancements in the delivery of NPs, endosomal escape remains a major issue and a matter of grave concern for developing an efficient and targeted delivery system for therapeutic applications. Most of NPs generally follow endocytic pathway for internalization into the cells. Read More

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http://dx.doi.org/10.1016/j.biochi.2019.02.012DOI Listing
May 2019
2.963 Impact Factor

Brain-derived neurotrophic factor induces thioredoxin-1 expression through TrkB/Akt/CREB pathway in SH-SY5Y cells.

Biochimie 2019 May 20;160:55-60. Epub 2019 Feb 20.

Laboratory of Molecular Neurobiology, Medical Faculty, Kunming University of Science and Technology, Kunming, 650500, China. Electronic address:

Brain-derived neurotrophic factor (BDNF) is one of the neurotrophic factors that are vital to the survival and proliferation of neuron. Thioredoxin-1 (Trx-1) is a redox regulating protein and plays various roles in regulating transcript factors and inhibiting apoptosis. It has been reported that Trx-1 is required for nerve growth factor-mediated signal transduction and neurite outgrowth, and is involved in synaptic protein expression induced by BDNF. Read More

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http://dx.doi.org/10.1016/j.biochi.2019.02.011DOI Listing

L-Dopa decarboxylase interaction with the major signaling regulator ΡΙ3Κ in tissues and cells of neural and peripheral origin.

Biochimie 2019 May 20;160:76-87. Epub 2019 Feb 20.

Molecular Virology Laboratory, Hellenic Pasteur Institute, 127 Vas. Sofias ave., 11521, Athens, Greece. Electronic address:

L-Dopa decarboxylase (DDC) catalyzes the decarboxylation of L-Dopa to dopamine and 5-hydroxytryptophan (5-HTP) to serotonin. Although DDC has been purified from a variety of peripheral organs, including the liver, kidney and pancreas, the physiological significance of the peripherally expressed enzyme is not yet fully understood. DDC has been considered as a potential novel biomarker for various types of cancer, however, the role of DDC in the development of hepatocellular carcinoma (HCC) remains to be evaluated. Read More

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http://dx.doi.org/10.1016/j.biochi.2019.02.009DOI Listing
May 2019
2 Reads

Corrigendum to "Regulation of β-catenin stabilization in human platelets" [Biochimie 95 (6) (2013) 1252-1257].

Biochimie 2019 Feb 18. Epub 2019 Feb 18.

Department of Biochemistry, Institute of Medical Sciences, Banaras Hindu University, Varanasi, 221005, UP, India. Electronic address:

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http://dx.doi.org/10.1016/j.biochi.2019.02.001DOI Listing
February 2019

A minimum set of stable blocks for rational design of polypeptide chains.

Biochimie 2019 May 19;160:88-92. Epub 2019 Feb 19.

Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilov St. 32, 119991, Moscow, Russia. Electronic address:

The aim of this work was to find a minimal set of structurally stable pentapeptides, which allows forming a polypeptide chain of a required 3D structure. To search for factors that ensure structural stability of the pentapeptide, we generated peptide sequences with no more than three functional groups, based on the alanine pentapeptide AAAAA. We analyzed 44,860 structures of peptides by the molecular dynamics method and found that 1,225 pentapeptides over 80% of the simulation time were in a stable conformation. Read More

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http://dx.doi.org/10.1016/j.biochi.2019.02.006DOI Listing

Microcin J25 inhibits ubiquinol oxidase activity of purified cytochrome bd-I from Escherichia coli.

Biochimie 2019 May 19;160:141-147. Epub 2019 Feb 19.

Instituto Superior de Investigaciones Biológicas (INSIBIO, CONICET-UNT) e Instituto de Química Biológica "Dr. Bernabé Bloj", Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, Chacabuco 461, San Miguel de Tucumán, Argentina. Electronic address:

Microcin J25 (MccJ25), an antimicrobial peptide, targets the respiratory chain but the exact mechanism by which it does so remains unclear. Here, we reveal that MccJ25 is able to inhibit the enzymatic activity of the isolated cytochrome bd-I from E. coli and induces at the same time production of reactive oxygen species. Read More

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http://dx.doi.org/10.1016/j.biochi.2019.02.007DOI Listing