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    SUMO1/sentrin/SMT3 specific peptidase 2 modulates target molecules and its corresponding functions.
    Biochimie 2018 Jun 13. Epub 2018 Jun 13.
    Department of Oncology, Shanghai 9th People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 201900, China. Electronic address:
    Small ubiquitin-like modifier (SUMOylation) is a reversible post-translational modification, which plays important roles in numerous biological processes. SUMO could be covalently attached to target proteins in an isopeptide bond manner that occurs via a lysine ε-amino group on the target proteins and the glycine on SUMO C-terminus. This covalent binding could affect the subcellular localization and stability of target proteins. Read More

    Screening and characterization of a Annenix A2 binding aptamer that inhibits the proliferation of myeloma cells.
    Biochimie 2018 Jun 12. Epub 2018 Jun 12.
    Molecular Biology Research Center, School of Life Sciences, Central South University, Changsha, 410078, China. Electronic address:
    Multiple myeloma (MM) is a malignant plasma cell disease and is considered incurable. Annexin A2 (ANXA2) is closely related to the proliferation and adhesion of MM. Using protein-SELEX, we performed a screen for aptamers that bind GST-ANXA2 from a library, and GST protein was used for negative selection. Read More

    Implication of oxysterols in chronic inflammatory human diseases.
    Biochimie 2018 Jun 9. Epub 2018 Jun 9.
    Department of Clinical and Biological Sciences, University of Turin, San Luigi Hospital, 10043, Orbassano, Turin, Italy. Electronic address:
    A growing bulk of evidence suggests that cholesterol oxidation products, known as oxysterols, are potentially involved in the pathogenesis of major chronic diseases, including atherosclerosis, Alzheimer's disease, and inflammatory bowel disease. Oxysterols are involved in various key steps of these complex processes, mainly thanks to their ability to act through up-regulation of oxidative stress, inflammation, and cell toxicity. This review summarizes the current knowledge of the effects induced by these compounds on cells, after their accumulation in the arterial wall, brain, and intestine. Read More

    Substituted Phosphonic Analogues of Phenylglycine as Inhibitors of Phenylalanine Ammonia Lyase from Potatoes.
    Biochimie 2018 Jun 8. Epub 2018 Jun 8.
    Department of Bioorganic Chemistry, Faculty of Chemistry, Wrocław University of Science and Technology, Wybrzeże Wyspiańskiego 27, 50-370, Wrocław, Poland. Electronic address:
    A series of phosphonic acid analogues of phenylglycine variously substituted in phenyl ring have been synthesized and evaluated for their inhibitory activity towards potato L-phenylalanine ammonia lyase. Most of the compounds appeared to act as moderate (micromolar) inhibitors of the enzyme. Analysis of their binding performed using molecular modeling have shown that they might be bound either in active site of the enzyme or in the non-physiologic site. Read More

    Emerging fields in chaperone proteins: a French workshop.
    Biochimie 2018 Jun 8. Epub 2018 Jun 8.
    Aix Marseille Univ, CNRS, BIP, Bioénergétique et Ingénierie des Protéines, Marseille, France. Electronic address:
    The "Bioénergétique et Ingénierie des Protéines (BIP)" laboratory, CNRS (France), organized its first French workshop on molecular chaperone proteins and protein folding in November 2017. The goal of this workshop was to gather scientists working in France on chaperone proteins and protein folding. This initiative was a great success with excellent talks and fruitful discussions. Read More

    G4RNA screener web server: User focused interface for RNA G-quadruplex prediction.
    Biochimie 2018 Jun 6. Epub 2018 Jun 6.
    RNA Group/Groupe ARN, Département de Biochimie, Faculté de Médecine et des Sciences de la Santé, Pavillon de Recherche Appliquée au Cancer, Université de Sherbrooke, 3201 rue Jean-Mignault, Sherbrooke, Québec, J1E 4K8, Canada. Electronic address:
    Though RNA G-quadruplexes became a focus of study over a decade ago, the main challenge associated with the identification of new potential G-quadruplexes remains a bottleneck step. It slows the study of these non-canonical structures in nucleic acids, and thus the understanding of their significance. The G4RNA screener is an accurate tool for the prediction of RNA G-quadruplexes but its deployment has brought to light an issue with its accessibility to G-quadruplex experts and biologists. Read More

    Inflammation induced mTORC2-Akt-mTORC1 signaling promotes macrophage foam cell formation.
    Biochimie 2018 Jun 5. Epub 2018 Jun 5.
    Metabolic Disease Biology Laboratory, Department of Molecular Biology and Biotechnology, Tezpur University, Tezpur, 784028, Assam, India. Electronic address:
    The transformation of macrophages into lipid loaded foam cells is a critical and early event in the pathogenesis of atherosclerosis. Several recent reports highlighted that induction of TLR4 signaling promotes macrophage foam cell formation; however, the underlying molecular mechanisms have not been clearly elucidated. Here, we found that the TLR4 mediated inflammatory signaling communicated with mTORC2-Akt-mTORC1 metabolic cascade in macrophage and thereby promoting lipid uptake and foam cell formation. Read More

    Consecutive Ribonucleoside Monophosphates on Template Inhibit DNA Replication by T7 DNA Polymerase or by T7 Polymerase and Helicase Complex.
    Biochimie 2018 Jun 5. Epub 2018 Jun 5.
    Public Health Laboratory Sciences and Toxicology, West China School of Public Health, Sichuan University, Chengdu, China. Electronic address:
    rNTPs are structurally similar to dNTPs but are largely molar excessive in cell than dNTPs. rNTPs are inevitably incorporated into DNA to form rNMPs. Long RNA primers can also be incorporated into lagging-strand DNA. Read More

    Cysteine cathepsins as a prospective target for anticancer therapies-current progress and prospects.
    Biochimie 2018 Jun 2. Epub 2018 Jun 2.
    Department of Biology and Pharmaceutical Botany, Medical University of Gdansk, Hallera 107, 80-416 Gdansk, Poland.
    Cysteine cathepsins (CTS), being involved in both physiological and pathological processes, play an important role in the human body. During the last 30 years, it has been shown that CTS are highly upregulated in a wide variety of cancer types although they have received a little attention as a potential therapeutic target as compared to serine or metalloproteinases. Studies on the increasing problem of neoplastic progression have revealed that secretion of cell-surface- and intracellular cysteine proteases is aberrant in tumor cells and has an impact on their growth, invasion, and metastasis by taking part in tumor angiogenesis, in apoptosis, and in events of inflammatory and immune responses. Read More

    Expression of long non-coding RNA LINC00973 is consistently increased upon treatment of colon cancer cells with different chemotherapeutic drugs.
    Biochimie 2018 Jun 2. Epub 2018 Jun 2.
    Engelhardt Institute of Molecular Biology, the Russian Academy of Sciences, 119991, Moscow, Russia. Electronic address:
    Early prediction of tumor relapse depends on the identification of new prognostic cancer biomarkers, which are suitable for monitoring tumor response to different chemotherapeutic drugs. Using RNA-Seq, RT-qPCR, bioinformatics, and studies utilizing the murine tumor xenograft model, we have found significant and consistent changes in the abundance of five lincRNAs (LINC00973, LINC00941, CASC19, CCAT1, and BCAR4) upon treatment of both HT-29 and HCT-116 cells with 5-fluorouracil, oxaliplatin, and irinotecan at different doses and durations; both in vitro and in vivo. The most frequent changes were detected for LINC00973, whose content is most strongly and consistently increased upon treatment of both colon cancer cell lines with all three chemotherapeutic drugs. Read More

    Enhanced expression and distinctive characterization of a long-acting FGF21 and its potential to alleviate nonalcoholic steatohepatitis.
    Biochimie 2018 Jun 2. Epub 2018 Jun 2.
    Jiangsu Key Laboratory of Druggability of Biopharmaceuticals, State Key Laboratory of Natural Medicines, School of Life Science and Technology, China Pharmaceutical University, Nanjing, China. Electronic address:
    We have previously constructed a novel polypeptide, PsTag, that should be useful in the development of biologics with properties comparable to those achievable by PEGylation, but with potentially less side effects. However, the low fermentation yields of polypeptide fusion proteins may limit the application of this technology. We suspected that when polypeptide fusion protein was expressed in E. Read More

    A novel fully human anti-CD47 antibody as a potential therapy for human neoplasms with good safety.
    Biochimie 2018 Jun 1. Epub 2018 Jun 1.
    Beijing Institute of Biotechnology, 20 Dongdajie Street, Fengtai District, Beijing, 100071, China. Electronic address:
    Strategies for targeting CD47 are becoming a hot spot of cancer immunotherapy. However the ubiquitous expression of CD47, especially on the RBC, makes the targeted therapy facing safety risk issues. So, how to balance the safety and efficacy during CD47 inhibition is currently a major question. Read More

    Leishmania mexicana gp63 is the enzyme responsible for cyclooxygenase (COX) activity in this parasitic protozoa.
    Biochimie 2018 Jun 1. Epub 2018 Jun 1.
    Departamento de Infectómica y Patogénesis Molecular, CINVESTAV-IPN, México, D.F, Mexico. Electronic address:
    Cyclooxygenase-2 (COX-2) is an enzyme responsible of prostaglandins production, such as prostaglandin E (PGE), an immune response modulator that regulates the immune system to inhibit Th1 and to promote Th2 cytokines production. Many parasites modulate their host immune response through PGE effects; however, in parasites, only one protein with COX activity has been described, the α-actinin of Entamoeba histolytica. Prostanoids production has been reported in some species of Leishmania but not the enzymes responsible of their production. Read More

    Secretome within the bone marrow microenvironment: A basis for mesenchymal stem cell treatment and role in cancer dormancy.
    Biochimie 2018 May 31. Epub 2018 May 31.
    Department of Medicine - Division of Hematology/Oncology, Rutgers, New Jersey Medical School, Newark, NJ 07103, USA. Electronic address:
    The secretome produced by cells within the bone marrow is significant to homeostasis. The bone marrow, a well-studied organ, has multiple niches with distinct roles for supporting stem cell functions. Thus, an understanding of mediators involved in the regulation of stem cells could serve as a model for clinical problems and solutions such as tissue repair and regeneration. Read More

    Diacylglycerol kinase δ controls down-regulation of cyclin D1 for C2C12 myogenic differentiation.
    Biochimie 2018 May 30. Epub 2018 May 30.
    Department of Chemistry, Graduate School of Science, Chiba University, Chiba, Japan. Electronic address:
    Diacylglycerol kinase (DGK) is a lipid-metabolizing enzyme that phosphorylates diacylglycerol (DG) to produce phosphatidic acid (PA). DGKδ is highly expressed in the skeletal muscle, and a decrease in DGKδ expression increases the severity of type 2 diabetes. However, the role of DGKδ in myogenic differentiation is still unknown. Read More

    Secretome from SH-SY5Y APP cells trigger time-dependent CHME3 microglia activation phenotypes, ultimately leading to miR-21 exosome shuttling.
    Biochimie 2018 May 29. Epub 2018 May 29.
    Neuron Glia Biology in Health and Disease, Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, Lisboa, Portugal; Department of Biochemistry and Human Biology, Faculty of Pharmacy, Universidade de Lisboa, Lisboa, Portugal. Electronic address:
    Exosome-mediated intercellular communication has been increasingly recognized as having a broad impact on Alzheimer's disease (AD) pathogenesis. Still, limited information exists regarding their "modus operandi", as it critically depends on exosomal cargo, environmental context and target cells. Therefore, a more thorough understanding of the role of exosomes from different cell types as mediators of neuroinflammation in AD context is a decisive step to open avenues for innovative and efficient therapies. Read More

    Quercetin inhibits glucose transport by binding to an exofacial site on GLUT1.
    Biochimie 2018 May 29. Epub 2018 May 29.
    Department of Chemistry and Biochemistry, Calvin College, Grand Rapids, MI, 49546, USA. Electronic address:
    Quercetin, a common dietary flavone, is a competitive inhibitor of glucose uptake and is also thought to be transported into cells by GLUT1. In this study, we confirm that quercetin is a competitive inhibitor of GLUT1 and also demonstrate that newly synthesized compounds, WZB-117 and BAY-876 are robust inhibitors of GLUT1 in L929 cells. To measure quercetin interaction with L929 cells, we develop a new fluorescent assay using flow cytometry. Read More

    MyPMFs: a simple tool for creating statistical potentials to assess protein structural models.
    Biochimie 2018 May 29. Epub 2018 May 29.
    Sorbonne Université, UMR 7590 CNRS, MNHN, IRD, Institut de Minéralogie de Physique des Matériaux et de Cosmochimie (IMPMC), Paris, France.
    Evaluating the model quality of protein structures that evolve in environments with particular physicochemical properties requires scoring functions that are adapted to their specific residue compositions and/or structural characteristics. Thus, computational methods developed for structures from the cytosol cannot work properly on membrane or secreted proteins. Here, we present MyPMFs, an easy-to-use tool that allows users to train statistical potentials of mean force (PMFs) on the protein structures of their choice, with all parameters being adjustable. Read More

    Exosomes in lung cancer diagnosis and treatment. From the translating research into future clinical practice.
    Biochimie 2018 May 29. Epub 2018 May 29.
    First Department of Pathology, National and Kapodistrian University of Athens, Athens, Greece. Electronic address:
    Lung cancer is one of the main causes of cancer-related death worldwide. Despite advances in lung cancer pathophysiology, diagnosis and prognosis, a better understanding of the disease is strongly needed in order to establish novel diagnostic and therapeutic approaches that should improve treatment outcomes. Exosomes are a type of cell-secreted extracellular vesicles, which transfer a wide variety of biomolecules, such as proteins, mRNAs, microRNAs, and lipids, are implicated in intercellular communication and modulate tumor-host interactions. Read More

    Non-stereoselective decomposition of (±)-S-alk(en)yl-L-cysteine sulfoxides to antibacterial thiosulfinates catalyzed by C115H mutant methionine γ-lyase from Citrobacter freundii.
    Biochimie 2018 May 24. Epub 2018 May 24.
    Engelhardt Institute of Molecular Biology of the Russian Academy of Sciences, Moscow, Russia. Electronic address:
    S-Alk(en)yl-L-cysteine sulfoxides, initially found in plants of the genus Allium, are converted to antimicrobial thiosulfinates by pyridoxal 5'-phosphate(PLP)-dependent alliinase (EC 4.4.1. Read More

    Trans-anethole ameliorates obesity via induction of browning in white adipocytes and activation of brown adipocytes.
    Biochimie 2018 May 24. Epub 2018 May 24.
    Department of Biotechnology, Daegu University, Gyeongsan, Gyeongbuk, 38453, Republic of Korea. Electronic address:
    To treat obesity, suppression of white adipose tissue (WAT) expansion and activation of brown adipose tissue (BAT) are considered as potential therapeutic targets. Recent advances have been made in the induction of brown fat-like adipocytes (beige) in WAT, which represents an attractive potential strategy for the management and treatment of obesity. Use of natural compounds for browning of white adipocytes can be considered as a safe and novel strategy against obesity. Read More

    Proteomic analysis of Tn-bearing glycoproteins from different stages of melanoma cells reveals new biomarkers.
    Biochimie 2018 May 23. Epub 2018 May 23.
    Department of Glycoconjugate Biochemistry, Institute of Zoology and Biomedical Research, Jagiellonian University, Gronostajowa 9, 30-387, Krakow, Poland. Electronic address:
    Cutaneous melanoma, the most aggressive form of skin cancer, responds poorly to conventional therapy. The appearance of Tn antigen-modified proteins in cancer is correlated with metastasis and poor prognoses. The Tn determinant has been recognized as a powerful diagnostic and therapeutic target, and as an object for the development of anti-tumor vaccine strategies. Read More

    Chlorogenic acid ameliorates alcohol-induced liver injuries through scavenging reactive oxygen species.
    Biochimie 2018 Jul 19;150:131-138. Epub 2018 May 19.
    School of Life Sciences, BK21 Plus KNU Creative BioResearch Group, College of Natural Sciences, Kyungpook National University, Taegu, Republic of Korea. Electronic address:
    The key role of oxidative stress in alcoholic liver disease (ALD) has been established by the large body of evidence from previous studies. Excessive consumption of ethanol induces the production of a variety of reactive oxygen species (ROS) in the liver, such as superoxide, HO, and hydroxyl radical. These products activate oxidant-sensitive signaling cascades and modulators of apoptosis. Read More

    A translational view of cells' secretome analysis - from untargeted proteomics to potential circulating biomarkers.
    Biochimie 2018 May 18. Epub 2018 May 18.
    CNC - Center for Neuroscience and Cell Biology, University of Coimbra, Portugal.
    The identification of circulating biomarkers capable of being used as routine for diagnosis, prognosis, risk stratification and therapeutic monitoring is still the major aim at clinical proteomics. However, the direct search in blood samples or other clinical relevant biofluids is frequently associated with technical limitations that make the biomarker discovery process extremely difficult when using these types of samples. In this sense, the use of different approaches, such as the analysis of cells' secretomes, can be an important and promising complementary method for the identification and pre-selection of potential circulating biomarkers. Read More

    Type I collagen induces mesenchymal cell differentiation into myofibroblasts through YAP-induced TGF-β1 activation.
    Biochimie 2018 Jul 17;150:110-130. Epub 2018 May 17.
    China-Japan Research Institute of Medical Pharmaceutical Sciences, Shenyang Pharmaceutical University, Shenyang, 110016, China. Electronic address:
    In organ fibrosis, mechanical stress and transforming growth factor beta-1 (TGF-β1) promote differentiation into myofibroblast from mesenchymal cells, leading to extracellular matrix (ECM) remodeling or active synthesis, deposition or degradation of ECM components. A major component of ECM, type I collagen (col I) triple helical molecules assemble into fibrils or are denatured to gelatin without triple-helicity in remodeling. However, whether changes of ECM components in remodeling have influence on mesenchymal cell differentiation remains elusive. Read More

    The direction of protein evolution is destined by the stability.
    Biochimie 2018 Jul 26;150:100-109. Epub 2018 May 26.
    Department of Biomolecular Chemistry, Kyoto Prefectural University, Hangi-cho, Shimogamo, Sakyo-ku, Kyoto 606-8522, Japan. Electronic address:
    Protein evolution is potentially governed by protein stability. Here, we investigated the relationship between protein evolution and stability through the random mutational drift of a thermophilic bacterial protein, an esterase of Alicyclobacillus acidocaldarius (Aac-Est), at high and low temperatures. In the first random mutation of Aac-Est, few proteins exhibit increased activity at 65 °C, indicating that the wild-type (WT) Aac-Est is located on the peak of a mountain in a fitness landscape for activity at high temperature. Read More

    The amyloidogenicity of a C-terminal region of TDP-43 implicated in Amyotrophic Lateral Sclerosis can be affected by anions, acetylation and homodimerization.
    Biochimie 2018 Jul 8;150:76-87. Epub 2018 May 8.
    Department of Biotechnology, Indian Institute of Technology Hyderabad, Kandi, Sangareddy, Telangana, 502285, India. Electronic address:
    Amyotrophic Lateral Sclerosis (ALS) is a fatal neurodegenerative disease associated with accumulation of hyper-phosphorylated, and ubiquitinated TAR DNA-binding protein-43 (TDP-43) as inclusion deposits in neuronal cells. Recently, amyloid-like fibrillar aggregates of TDP-43 have been reported from several ALS patients. The C-terminal region of TDP-43 is central to TDP-43's pathological aggregation and most of the familial ALS mutations in the encoding TARDBP gene are located in this domain. Read More

    Chromatography of oxysterols.
    Biochimie 2018 May 9. Epub 2018 May 9.
    Department of Chemistry, University of Oslo, PO Box 1033, Blindern, NO-0315, Oslo, Norway. Electronic address:
    Oxysterols play important roles in development and diseases, but can be highly challenging to analyze. To ensure satisfactory measurements, oxysterols must typically be separated with chromatography prior to detection. Here, we will devote attention to the chromatography of oxysterols, focusing on gas chromatography and liquid chromatography. Read More

    Importance of genetic background of oxysterol signaling in cancer.
    Biochimie 2018 May 7. Epub 2018 May 7.
    Toxicogenomics Unit, National Institute of Public Health, Srobarova 48, 100 42, Prague 10, Czech Republic; Biomedical Center, Faculty of Medicine in Pilsen, Charles University, Alej Svobody 76, Pilsen, 32300, Czech Republic. Electronic address:
    Oxysterols, oxygenated derivatives of cholesterol, are formed in the human body or ingested. Experimental evidence suggests that due to their diverse functions, e.g. Read More

    Bioinformatics analyses and in vitro evidence for five and six stacked G-quadruplex forming sequences.
    Biochimie 2018 Jul 4;150:70-75. Epub 2018 May 4.
    Department of Biology and Ecology/Institute of Environmental Technologies, Faculty of Science, University of Ostrava, Chittussiho 10, Ostrava, CZ, 71000, Czech Republic. Electronic address:
    Quadruplexes are noncanonical DNA structures that arise in guanine rich loci and have important biological functions. Classically, quadruplexes contain four stacked intramolecular G-tetrads. Surprisingly, although some algorithms allow searching for longer than 4G tracts for quadruplex formation, these have not yet been systematically studied. Read More

    Sabellastarte magnifica Carboxypeptidase Inhibitor: The first Kunitz inhibitor simultaneously interacting with carboxypeptidases and serine proteases.
    Biochimie 2018 Jul 3;150:37-47. Epub 2018 May 3.
    Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Av. Carlos Chagas Filho, 373 - Bloco E, sala 10, Rio de Janeiro, RJ, 21941-902, Brazil. Electronic address:
    Multi-domain inhibitors capable to block the activity of different classes of proteases are not very common in nature. However, these kinds of molecules are attractive systems for biomedical or biotechnological applications, where two or more different targets need to be neutralized. SmCI, the Sabellastarte magnifica Carboxypeptidase Inhibitor, is a tri-domain BPTI-Kunitz inhibitor capable to inhibit serine proteases and A-like metallocarboxypeptidases. Read More

    Phosphorylation-induced conformational changes of photoactivated rhodopsin probed by fluorescent labeling at Cys and Cys.
    Biochimie 2018 Jul 4;150:57-69. Epub 2018 May 4.
    Departamento de Biología Celular, Universidad Simón Bolívar, Caracas, Venezuela. Electronic address:
    In order to monitor conformational changes following photoactivation and phosphorylation of bovine rhodopsin, the two reactive sulfhydryl groups at Cys and Cys were specifically labeled with the monobromobimane (mBBr) fluorophore. Although alterations in conformation after light exposure of rhodopsin were not detected by fluorescence excitation scans (300-450 nm) of the mBBr-labeled protein, the fluorescence signal was reduced ∼ 90% in samples containing photoactivated phosphorhodopsin. Predominant labeling at either Cys or Cys in light-activated and phosphorylated rhodopsin merely generated a decrease of ∼38% and 28%, respectively, in the fluorescence excitation intensity. Read More

    Unusual interaction of human apurinic/apyrimidinic endonuclease 1 (APE1) with abasic sites via the Schiff-base-dependent mechanism.
    Biochimie 2018 Jul 3;150:88-99. Epub 2018 May 3.
    Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia; Novosibirsk State University, Novosibirsk, Russia. Electronic address:
    Clustered apurinic/apyrimidinic (AP) sites are more cytotoxic than isolated AP lesions because double strand breaks (DSB) can be formed during repair of closely positioned bistranded AP sites. Formation of DSB due to simultaneous cleavage of bistranded AP sites may be regulated by proteins specifically interacting with this complex lesion. A set of AP DNA duplexes containing AP sites in both strands in different mutual orientation (BS-AP DNAs) was used for search in the extracts of human cells proteins specifically recognizing clustered AP sites. Read More

    Plasma oxysterol profiling in children reveals 24-hydroxycholesterol as a potential marker for Autism Spectrum Disorders.
    Biochimie 2018 May 4. Epub 2018 May 4.
    Vascular Biology and Mass Spectrometry Laboratory, Department of Medical Sciences and Biotechnology, University of Rome, Latina, Italy. Electronic address:
    Cholesterol and its oxygenated metabolites, such as oxysterols, are intensively investigated as potential players in the pathophysiology of brain disorder. Altered oxysterol levels have been described in patients with numerous neuropsychiatric disorders, including Alzheimer's disease, Amyotrophic Lateral Sclerosis, Parkinson's disease, X-linked adrenoleukodystrophy, and Smith-Lemli-Opitz Syndrome. Recent studies have shown that Autism Spectrum Disorders are associated with disruption of cholesterol metabolism. Read More

    Exploring the biophysical properties of phytosterols in the plasma membrane for novel cancer prevention strategies.
    Biochimie 2018 May 3. Epub 2018 May 3.
    School of Food Science and Nutrition, Faculty of Mathematics and Physical Sciences, University of Leeds, LS2 9JT, UK. Electronic address:
    Cancer is a global problem with no sign that incidences are reducing. The great costs associated with curing cancer, through developing novel treatments and applying patented therapies, is an increasing burden to developed and developing nations alike. These financial and societal problems will be alleviated by research efforts into prevention, or treatments that utilise off-patent or repurposed agents. Read More

    Biological and regulatory roles of acid-induced small RNA RyeC in Salmonella Typhimurium.
    Biochimie 2018 Jul 3;150:48-56. Epub 2018 May 3.
    School of Biotechnology, KIIT University, Bhubaneswar, 751024, India. Electronic address:
    Salmonella Typhimurium is an enteric pathogen that has evolved masterful strategies to enable survival under stress conditions both within and outside a host. The acid tolerance response (ATR) is one such mechanism that enhances the viability of acid adapted bacteria to lethal pH levels. While numerous studies exist on the protein coding components of this response, there is very little data on the roles of small RNAs (sRNAs). Read More

    Notch3 is involved in adipogenesis of human adipose-derived stromal/stem cells.
    Biochimie 2018 Jul 27;150:31-36. Epub 2018 Apr 27.
    School of Biological Sciences, Louisiana Tech University, Ruston, LA, 71272, USA. Electronic address:
    Human adipose-derived stromal/stem cells (hASCs) have tremendous therapeutic potential and the ability to offer insight into human development and disease. Here we subject human ASCs to siRNA-mediated knockdown of Notch3 cultured under both self-renewing and adipogenic differentiation conditions. Self-renewal was monitored by assessing viability and proliferation rates through staining and alamarBlue assays, respectively. Read More

    Increase in liver cytosolic lipases activities and VLDL-TAG secretion rate do not prevent the non-alcoholic fatty liver disease in cafeteria diet-fed rats.
    Biochimie 2018 Jul 26;150:16-22. Epub 2018 Apr 26.
    Laboratory of Physiology, Federal University of São João del-Rei, Divinópolis, Minas Gerais, Brazil. Electronic address:
    We have previously shown that the cafeteria diet increases body fat mass, plasma triacylglycerol (TAG) and insulin levels, glucose uptake by white and brown adipose tissues, as well as the sympathetic activity to both adipose tissues in Wistar rats. The metabolic pathways responsible for the development of non-alcoholic fatty liver disease (NAFLD) were examined in cafeteria diet-fed rats. After 3 weeks offering cafeteria diet, we evaluated: (i) activity of the sympathetic nervous system by norepinephrine turnover rates; (ii) de novo fatty acid synthesis in vivo using HO; (iii) secretion of very low density lipoprotein (VLDL)-TAG secretion measuring serum TAG levels after administration of lipase lipoprotein inhibitor, (iv) liver cytosolic lipases activities and (v) liver mRNA expression of enzymes involved in lipids secretion and oxidation by RT-PCR. Read More

    Determination of the binding properties of p-cresyl glucuronide to human serum albumin.
    Biochimie 2018 Jul 26;150:1-7. Epub 2018 Apr 26.
    Univ. Lyon, CarMeN, INSERM U1060, INRA U1397, INSA de Lyon, Université Claude Bernard Lyon 1, F-69621 Villeurbanne, France. Electronic address:
    p-Cresyl glucuronide (p-CG) is a by-product of tyrosine metabolism that accumulates in patients with end-stage renal disease. p-CG binding to human serum albumin in physiological conditions (37 °C, pH 7.40) was studied by ultrafiltration (MWCO 10 kDa) and data were analyzed assuming one binding site. Read More

    Ophiopogonin D improves osteointegration of titanium alloy implants under diabetic conditions by inhibition of ROS overproduction via Wnt/β-catenin signaling pathway.
    Biochimie 2018 Apr 26. Epub 2018 Apr 26.
    Department of Orthopedics of the 463 Hospital of PLA, Shenyang, Liaoning, 110042, China. Electronic address:
    A high failure rate of titanium implants in diabetic patients has been indicated in clinical evidences. Excessive oxidative stress at the bone-implant interface plays an important role in the impaired osteointegration under diabetic conditions. While the underlying mechanisms remain unknown and the targeted treatments are urgently needed. Read More

    In vitro oxidized HDL and HDL from type 2 diabetes patients have reduced ability to efflux oxysterols from THP-1 macrophages.
    Biochimie 2018 Apr 26. Epub 2018 Apr 26.
    Inserm, U1060, CarMeN Laboratory, Villeurbanne, F-69621, France; INSA-Lyon, IMBL, Villeurbanne, F-69621, France; Université Lyon 1, Villeurbanne, F-69622, France. Electronic address:
    Oxidized LDL (OxLDL) that are enriched in products of lipid peroxidation including oxysterols have been shown to induce cellular oxidative stress and cytotoxicity therefore accelerating atheroma plaque formation. Upon oxLDL exposure of THP-1 macrophages, intracellular oxidation of LDL derived-cholesterol as well as endogenous cholesterol was increased. The oxysterols intracellularly produced were efficiently exported to HDL whereas apolipoprotein A1 was inefficient. Read More

    Cellular mechanism of resistance of human colorectal adenocarcinoma cells against apoptosis-induction by Russell's Viper venom l-amino acid oxidase (Rusvinoxidase).
    Biochimie 2018 Jul 25;150:8-15. Epub 2018 Apr 25.
    School of Biological Sciences, University of Northern Colorado, Greeley, CO, 80639-0017, USA. Electronic address:
    The present study highlights the cellular mechanism of resistance in human adenocarcinoma (Colo-205) cells against apoptosis induction by Rusvinoxidase, an l-amino acid oxidase purified from Russell's Viper venom (RVV). The significantly lower cytotoxicity as well as apoptotic activity of Rusvinoxidase towards Colo-205 cells (compared to MCF-7 breast cancer cells) is correlated with lower depletion of cellular glutathione content and increased down-regulation of catalase activity of Colo-205 cells following Rusvinoxidase treatment. Exposure to Rusvinoxidase subsequently diminished reactive oxygen species (ROS) production and failed to impair mitochondrial membrane potential, resulting in apoptosis induction resistance in Colo-205 cells. Read More

    MiR-98 suppresses the effects of tumor-associated macrophages on promoting migration and invasion of hepatocellular carcinoma cells by regulating IL-10.
    Biochimie 2018 Jul 24;150:23-30. Epub 2018 Apr 24.
    Department of Hepatobiliary and Pancreatic Surgery, Xuzhou Cancer Hospital, Xuzhou 221000, Jiangsu, China. Electronic address:
    We aim to investigate the role of miR-98-mediated macrophage polarization in hepatocellular carcinoma (HCC) progression and to explore the underlying mechanism. A total of 25 paired HCC and matched adjacent normal tissues (ANTs) were collected. We incubated human blood monocytes isolated from healthy male donors with culture medium collected from HepG2 cells for 7 days. Read More

    The pericyte secretome: Potential impact on regeneration.
    Biochimie 2018 Apr 23. Epub 2018 Apr 23.
    Translational Neurology Group, Department of Clinical Science, Wallenberg Neuroscience Center and Wallenberg Centre for Molecular Medicine, Lund University, 22184 Lund, Sweden; Department of Neurology, Scania University Hospital, 22185 Lund, Sweden. Electronic address:
    Personalized and regenerative medicine is an emerging therapeutic strategy that is based on cell biology and biomedical engineering used to develop biological substitutes to maintain normal function or restore damaged tissues and organs. The secretory capacities of different cell types are now explored as such possible therapeutic regenerative agents in a variety of diseases. A secretome can comprise chemokines, cytokines, growth factors, but also extracellular matrix components, microvesicles and exosomes as well as genetic material and may differ depending on the tissue and the stimulus applied to the cell. Read More

    EBI2 - Sensor for dihydroxycholesterol gradients in neuroinflammation.
    Biochimie 2018 Apr 22. Epub 2018 Apr 22.
    Institute for Molecular Medicine, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany.
    Dihydroxycholesterols such as 7α,25-dihydroxysterols (7α,25-OHC) and 7α,27-OHC are generated from cholesterol by the enzymes CH25H, CYP7B1 and CYP27A1 in steady state but also in the context of inflammation. The G-protein coupled receptor (GPCR) Epstein-Barr virus-induced gene 2 (EBI2), also known as GPR183, senses these oxysterols and induces chemotactic migration of immune cells towards higher concentrations of these ligands. We recently showed that these ligands are upregulated in the CNS in experimental autoimmune encephalomyelitis (EAE), an animal model for multiple sclerosis and that EBI2 enhanced early infiltration of encephalitogenic T cells into the CNS. Read More

    Functional characterization and molecular modeling of the mutations in CYP21A2 gene from patients with Congenital Adrenal Hyperplasia.
    Biochimie 2018 Jun 22;149:115-121. Epub 2018 Apr 22.
    Department of Biochemistry, Postgraduate Institute of Medical Education and Research, Chandigarh, India. Electronic address:
    Steroid 21-Hydroxylase deficiency is an inherited autosomal recessive metabolic disorder of the adrenal steroidogenesis caused due to mutations in the CYP21A2 gene in 95% of CAH cases. Notably, the de novo mutations arise at the rate of 3-5%, therefore the functional characterization is of utmost importance for categorization of the novel mutations. Herein, we have functionally characterized the CYP21A2 missense mutations viz. Read More

    The effect of oxysterols on nerve impulses.
    Biochimie 2018 Apr 21. Epub 2018 Apr 21.
    University of Bourgogne Franche-Comté, Team 'Biochemistry of the Peroxisome, Inflammation and Lipid Metabolism', EA 7270 / Inserm, Dijon, France. Electronic address:
    The propagation of nerve impulses in myelinated nerve fibers depends on a number of factors involving the myelin and neural axons. In several neurodegenerative diseases, nerve impulses can be affected by the structural and biochemical characteristics of the myelin sheath and the activity of ion channels located in the nodes of Ranvier. Though it is generally accepted that lipid disorders are involved in the development of neurodegenerative diseases, little is known about their impact on nerve impulses. Read More

    Exposing mesenchymal stem cells to chondroitin sulphated proteoglycans reduces their angiogenic and neuro-adhesive paracrine activity.
    Biochimie 2018 Apr 19. Epub 2018 Apr 19.
    Biological Sciences, Faculty of Medicine, Dentistry and Life Sciences, University of Chester, Parkgate Road, Chester, CH1 4BJ, United Kingdom. Electronic address:
    The multifactorial complexity of spinal cord injuries includes the formation of a glial scar, of which chondroitin sulphated proteoglycans (CSPG) are an integral component. Previous studies have shown CSPG to have inhibitory effects on endothelial and neuronal cell growth, highlighting the difficulty of spinal cord regeneration. Mesenchymal stem/stromal cells (MSC) are widely used as a cell therapy, and there is mounting evidence for their angiogenic and neurotrophic paracrine properties. Read More

    Corrigendum to 'Differentiation-dependent expression of interferon gamma and toll-like receptor 9 in 3T3-F442A adipocytes' [Biochimie 89 (5) (2007) 669-675].
    Biochimie 2018 Jun 17;149:139. Epub 2018 Apr 17.
    Institut National de la Santé et de la Recherche Médicale, unit 747 (Inserm UMR-S 747), Université Paris Descartes, Centre Universitaire des Saints-Peres, 45 rue des Saints-Peres, 75006 Paris, France. Electronic address:

    Biochemical and dynamic basis for combinatorial recognition of H3R2K9me2 by dual domains of UHRF1.
    Biochimie 2018 Jun 12;149:105-114. Epub 2018 Apr 12.
    Department of Biotechnology, Indian Institute of Technology Hyderabad, Kandi, Sangareddy, 502285, Telangana, India. Electronic address:
    UHRF1 is a multi-domain protein comprising of a tandem tudor (UHRF1 TTD), a PHD finger, and a SET and RING-associated domain. It is required for the maintenance of CG methylation, heterochromatin formation and DNA repair. Isothermal titration calorimetry binding studies of unmodified and methylated lysine histone peptides establish that the UHRF1 TTD binds dimethylated Lys9 on histone H3 (H3K9me2). Read More

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