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    Temozolomide Affects Extracellular Vesicles Released by Glioblastoma Cells.
    Biochimie 2018 Feb 14. Epub 2018 Feb 14.
    CRCINA, Team SOAP, Inserm, CNRS, Université de Nantes, Université D'Angers, France. Electronic address:
    Glioblastoma multiforme (GBM) is the most aggressive primary tumour within the brain as well as the most common and lethal cerebral cancer, mainly because of treatment failure. Indeed, tumour recurrence is inevitable and fatal in a short period of time. Glioblastoma stem-like cells (GSCs) are thought to participate in tumour initiation, expansion, resistance to treatments, including to the alkylating chemotherapeutic agent temozolomide, and relapse. Read More

    Sticholysin II-mediated cytotoxicity involves the activation of regulated intracellular responses that anticipates cell death.
    Biochimie 2018 Feb 13. Epub 2018 Feb 13.
    Center for Protein Studies, Biology Faculty, University of Havana, Cuba, CP10400. Electronic address:
    Sticholysin II (StII) is a pore-forming toxin of biomedical interest that belongs to the actinoporin protein family. Sticholysins are currently under examination as an active immunomodulating component of a vaccinal platform against tumoral cells and as a key element of a nucleic acids delivery system to cell cytosol. These proteins form pores in the plasma membrane leading to ion imbalance and cell lysis. Read More

    Biophysical methods: complementary tools to study the influence of human steroid hormones on the liposome membrane properties.
    Biochimie 2018 Feb 13. Epub 2018 Feb 13.
    Bioactive Molecules Research Laboratory, Faculty of Sciences, Lebanese University, Lebanon. Electronic address:
    Human steroid hormones are involved in many aspects of physiology and have long been known to exert rapid and delayed effects. They are lipophilic molecules which can be incorporated into the lipid membranes. Through non-covalent interactions they can alter the properties of the membrane, including fluidity, lipid raft formation and others. Read More

    G-quadruplexes in Prague: a Bohemian Rhapsody.
    Biochimie 2018 Feb 13. Epub 2018 Feb 13.
    School of Pharmacy, University of East Anglia, Norwich Research Park, Norwich, NR4 7TJ, UK; Centre for Molecular and Structural Biochemistry, University of East Anglia, Norwich Research Park, Norwich, NR4 7TJ, UK. Electronic address:
    The Sixth International Meeting on Quadruplex Nucleic Acids was held at the Hotel Internationale in Prague, Czech Republic from 31 May - 3 June 2017. A vibrant interdisciplinary community of almost over 300 scientists gathered to share their newest results in this exciting field and exchange ideas for further investigations. Read More

    Oleic acid induces the novel apolipoprotein O and reduces mitochondrial membrane potential in chicken and human hepatoma cells.
    Biochimie 2018 Feb 9. Epub 2018 Feb 9.
    Department of Medical Biochemistry, Max F. Perutz Laboratories, Medical University of Vienna, Vienna, Austria. Electronic address:
    Non-alcoholic fatty liver disease (NAFLD) is marked by hepatic fat accumulation and reflects a spectrum of chronic liver diseases associated with obesity, impaired insulin sensitivity and dyslipidemia. Apolipoprotein O (ApoO) is a new member of the plasma apolipoprotein family that may play a role in lipid metabolism and electron transport activity of the mitochondrium. However, its physiological functions have not been elucidated yet. Read More

    NK-18, a promising antimicrobial peptide: anti-multidrug resistant leukemia cells and LPS neutralizing properties.
    Biochimie 2018 Feb 7. Epub 2018 Feb 7.
    The Key Laboratory, The First Hospital of Lanzhou University, Lanzhou, 730000, China. Electronic address:
    With the increase of multidrug resistance, novel anti-leukemia agents with diverse mechanisms of action are required to address this challenge. NK-18, the core region of mammalian derived protein NK-lysin, effectively inhibited the viability of both multidrug resistant and sensitive leukemia cell lines. Meanwhile, this proliferation inhibition effect was not distinct between sensitive and multidrug resistant leukemia cell line. Read More

    Punicalagin, an active pomegranate component, is a new inhibitor of PDIA3 reductase activity.
    Biochimie 2018 Feb 6. Epub 2018 Feb 6.
    Department of Biochemical Sciences "A. Rossi Fanelli" and Istituto Pasteur-Fondazione Cenci Bolognetti, Sapienza University ", P.le A. Moro 5, 00185, Rome, Italy. Electronic address:
    Background: Polyphenolic compounds isolated from pomegranate fruit possess several pharmacological activities including anti-inflammatory, hepatoprotective, antigenotoxic and anticoagulant activities. The present work focuses the attention on PDIA3 interaction with punicalagin and ellagic acid, the most predominant components of pomegranate extracts. PDIA3, a member of the protein disulfide isomerase family involved in several cellular functions, is associated with different human diseases and it has the potential to be a pharmacological target. Read More

    Simulating the γ-secretase enzyme: recent advances and future directions.
    Biochimie 2018 Feb 1. Epub 2018 Feb 1.
    Facultad de Química, Departamento de Fisicoquímica, Universidad Nacional Autónoma de México, CDMX, México, 04510, Mexico. Electronic address:
    γ-secretase is an intra-membrane aspartyl protease involved in the production of amyloid-β peptides. Aberrant cleavage of 99-residue C-terminal fragment of the amyloid precursor protein leads to the formation of a 42-amino acid length of Aβ. Further oligomerization and aggregation of this isoform is implicated in the onset and progression of Alzheimer's disease. Read More

    Cloning and sequencing of three-finger toxins from the venom glands of four Micrurus species from Mexico and heterologous expression of an alpha-neurotoxin from Micrurus diastema.
    Biochimie 2018 Jan 29. Epub 2018 Jan 29.
    Instituto de Biotecnología, Universidad Nacional Autónoma de México, Av. Universidad # 2001, Colonia Chamilpa, CP: 62210 Cuernavaca, Morelos, Mexico. Electronic address:
    The three-finger toxins (3FTxs) represent an extremely diverse protein family in elapid venoms, where the short chain α-neurotoxins are the most relevant toxin group from the clinical point of view. Essentially, the 3FTxs variability and the low proportions of α-neurotoxins in the venoms of North American coral snakes make it difficult to obtain effective elapid antivenoms against the envenomation symptoms caused mainly by these α-neurotoxins. In this work, thirty 3FTx transcript sequences were obtained from the venom glands of four coral snake species from Mexico (M. Read More

    Functional attribution of LdISP, an endogenous serine protease inhibitor from Leishmania donovani in promoting infection.
    Biochimie 2018 Jan 20. Epub 2018 Jan 20.
    Department of Biochemistry and Biophysics, University of Kalyani, Kalyani 741235, West Bengal, India. Electronic address:
    Leishmaniasis, a parasitic disease caused by unicellular eukaryotic protozoa of the genus Leishmania, affects more than 12 million people worldwide. Events of leishmaniasis are based on the infection of the mammalian host, precisely macrophages, where both host and parasite derived proteases and endogenous inhibitors are significant. Pathogen derived protease inhibitors have generated considerable interest as they often act as an agent promoting infection and parasitic survivability. Read More

    Glycine betaine rather than acting only as an osmolyte also plays a role as regulator in cellular metabolism.
    Biochimie 2018 Jan 20. Epub 2018 Jan 20.
    Centro de Investigación en Alimentación y Desarrollo A.C., Apartado Postal 1735, Hermosillo 83000, Sonora, Mexico. Electronic address:
    For many years, glycine betaine (GB) has been widely studied as an osmolyte in plants and bacteria. In animal cells, GB is an osmolyte mainly in the kidneys, but in humans many studies have shown its role as a methyl donor in homocysteine metabolism in the liver. GB is also a protein stabilizer, and thus, it became known as an osmoprotector. Read More

    Sodium thiosulfate mediated cardioprotection against myocardial ischemia-reperfusion injury is defunct in rat heart with co-morbidity of vascular calcification.
    Biochimie 2018 Jan 20. Epub 2018 Jan 20.
    Vascular Biology Lab, SASTRA Deemed University, Thanjavur, India-613401. Electronic address:
    Sodium thiosulfate (STS) has shown promising effects in amelioration of myocardial ischemia-reperfusion injury (IR) in a rat model and is clinically useful in the treatment of chronic kidney disease (CKD) associated calciphylaxis. As the prevalence of cardiac complications is higher in CKD, we tested the effectiveness of STS in a rat model of adenine-induced vascular calcification and subjected the heart to IR. We observed an increased infarct size (29%) by TTC staining, lactate dehydrogenase (54%) and creatine kinase (32%) release in the coronary perfusate and altered hemodynamics compared to a normal rat treated with STS and subjected to IR. Read More

    MiR-143-3p inhibits the proliferation, cell migration and invasion of human breast cancer cells by modulating the expression of MAPK7.
    Biochimie 2018 Jan 19. Epub 2018 Jan 19.
    Department of Oncology, Affiliated Hospital of Jining Medical University, Jining 272029, Shandong, China.
    Micro-RNAs have been reported to play crucial roles in a diversity of cellular processes such as cell proliferation, differentiation and development by regulating the expression of specific genes. They have also been shown to play vital roles in several diseases such as cancer. In the present study, we investigated the role of miR-143-3p in breast cancer. Read More

    Biochemical characterization of Campylobacter jejuni PNPase, an exoribonuclease important for bacterial pathogenicity.
    Biochimie 2018 Jan 12. Epub 2018 Jan 12.
    Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Ava da República, 2780-157 Oeiras, Portugal. Electronic address:
    Bacteria need to promptly respond to environmental changes. Ribonucleases (RNases) are key factors in the adaptation to new environments by enabling a rapid adjustment in RNA levels. The exoribonuclease polynucleotide phosphorylase (PNPase) is essential for low-temperature cell survival, affects the synthesis of proteins involved in virulence and has an important role in swimming, cell adhesion/invasion ability, and chick colonization in C. Read More

    Biosensors for Alzheimer's disease biomarker detection: A review.
    Biochimie 2018 Jan 4. Epub 2018 Jan 4.
    Hubei Province Key Laboratory of Occupational Hazard Identification and Control, Medical College, Wuhan University of Science and Technology, Wuhan 430065, PR China. Electronic address:
    Alzheimer's disease (AD) is a chronic disease amongst people aged 65 and older. Increasing evidence has illustrated that early diagnosis holds the key to effective treatment of AD. A variety of detection techniques have been developed. Read More

    Inhibition of autophagy reverses alcohol-induced hepatic stellate cells activation through activation of Nrf2-Keap1-ARE signaling pathway.
    Biochimie 2018 Jan 2. Epub 2018 Jan 2.
    Department of Gastroenterology, The Second Affiliated Hospital of Nanchang University, Nanchang; 330006, China.
    Background: Numerous documents have indicated a critical role of autophagy in alcoholic liver fibrosis (ALF), but few papers have reported its function in hepatic stellate cells (HSCs) activation. The current study aimed to investigate the regulation effect of autophagy in HSCs activation, in further to explore the underlying mechanism involved.

    Methods: HSC-T6 cells were treated with ethanol, 3-MA (autophagy inhibitor) or rapamycin (autophagy inducer), and cells were also transfected with si-Nrf2 or si-Keap1. Read More

    Direct visualization of the oligomeric state of hemagglutinins of influenza virus by high-resolution atomic force microscopy.
    Biochimie 2018 Mar 2;146:148-155. Epub 2018 Jan 2.
    Chemistry Department of Lomonosov Moscow State University, Moscow, Russian Federation. Electronic address:
    High-resolution atomic force microscopy (AFM) is a powerful technique for the direct visualization of single molecules. Here, AFM is applied to characterize the oligomeric state of hemagglutinins of the influenza virus. Hemagglutinins are known to be present in a trimeric form inside the viral envelope. Read More

    ssDNA Hybridization Facilitated by T7 ssDNA Binding Protein (gp2.5) Rapidly Initiates from the Strand Terminus or Internally Followed by a Slow Zippering Step.
    Biochimie 2017 Dec 29. Epub 2017 Dec 29.
    Public health laboratory sciences and toxicology, West China school of Public Health, Sichuan University, Chengdu, China. Electronic address:
    The hybridization of two complementary ssDNA is essential for molecular biology and biological physics. T7 ssDNA binding protein (gp2.5) can rapidly facilitate this hybridization, but the mechanism and kinetic process remain unknown. Read More

    L-ascorbic acid: a true substrate for HIF proly hydroxylase?
    Biochimie 2017 Dec 28. Epub 2017 Dec 28.
    Department of Chemical Enzymology, School of Chemistry, M.V.Lomonosov Moscow State University, Moscow 119992, Russia; A.N. Bach Institute of Biochemistry RAS, Leninsky prospect 33, 119071 Moscow, Russia; Innovation and High Technologies MSU Ltd, Tsymlyanskaya, 16, of 96, Moscow 109599, Russian Federation.
    L-Ascorbate (L-Asc), but not D-isoascorbate (D-Asc) and N-acetylcysteine (NAC) suppress HIF1 ODD-luc reporter activation induced by various inhibitors of HIF prolyl hydroxylase (PHD). The efficiency of suppression by L-Asc was sensitive to the nature of HIF PHD inhibitor chosen for reporter activation. In particular, the inhibitors developed to compete with alpha-ketoglutarate (αKG), were less sensitive to suppression by the physiological range of L-Asc (40-100 μM) than those having a strong iron chelation motif. Read More

    The gene product of a Trypanosoma equiperdum ortholog of the cAMP-dependent protein kinase regulatory subunit is a monomeric protein that is not capable of binding cyclic nucleotides.
    Biochimie 2018 Mar 27;146:166-180. Epub 2017 Dec 27.
    Department of Chemistry, Biochemistry and Pharmacology, University of California San Diego, La Jolla, CA 92093-0654, USA. Electronic address:
    The full gene sequence encoding for the Trypanosoma equiperdum ortholog of the cAMP-dependent protein kinase (PKA) regulatory (R) subunits was cloned. A poly-His tagged construct was generated [TeqR-like(His)], and the protein was expressed in bacteria and purified to homogeneity. The size of the purified TeqR-like(His)was determined to be ∼57,000 Da by molecular exclusion chromatography indicating that the parasite protein is a monomer. Read More

    Membrane permeabilization design of antimicrobial peptides based on chikungunya virus fusion domain scaffold and its antibacterial activity against gram-positive Streptococcus pneumoniae in respiratory infection.
    Biochimie 2018 Mar 24;146:139-147. Epub 2017 Dec 24.
    Department of Respiratory Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China.
    The structural dynamics of membrane permeabilization are investigated systematically and compared between viral fusion peptides (VFPs) and antimicrobial peptides (AMPs). It is revealed that the permeabilization process can be divided into two phases: a fast motion phase in water (first phase) and a slow diffusion phase in lipid (second phase). Difference in peptide permeability to neutrally or weakly charged mammalian membrane and to negatively charged bacterial membrane is primarily determined by the first phase, which is dominated by the direct electrostatic interaction between peptide and the hydrophilic surface of membranes. Read More

    Effect of dC → d(mC) substitutions on the folding of intramolecular triplexes with mixed TAT and CGC base triplets.
    Biochimie 2018 Mar 24;146:156-165. Epub 2017 Dec 24.
    Department of Pharmaceutical Sciences, University of Nebraska Medical Center, 986025 Nebraska Medical Center, Omaha, NE, 68198-6025, USA. Electronic address:
    Oligonucleotide-directed triple helix formation has been recognized as a potential tool for targeting genes with high specificity. Cystosine methylation in the 5' position is both ubiquitous and a stable regulatory modification, which could potentially stabilize triple helix formation. In this work, we have used a combination of calorimetric and spectroscopic techniques to study the intramolecular unfolding of four triplexes and two duplexes. Read More

    An archaeal aminoacyl-tRNA synthetase complex for improved substrate quality control.
    Biochimie 2017 Dec 19. Epub 2017 Dec 19.
    Department of Chemistry, Faculty of Science, University of Zagreb, Horvatovac 102A, 10000 Zagreb, Croatia. Electronic address:
    Aminoacyl-tRNA synthetases (aaRS) decode genetic information by coupling tRNAs with cognate amino acids. In the archaeon Methanothermobacter thermautotrophicus arginyl- and seryl-tRNA synthetase (ArgRS and SerRS, respectively) form a complex which enhances serylation and facilitates tRNArecycling through its association with the ribosome. Yet, the way by which complex formation participates in Arg-tRNAsynthesis is still unresolved. Read More

    Deoxynucleoside 5-monophosphate N-glycosidase from a phylogenetically distant metazoa, sponge.
    Biochimie 2018 Mar 19;146:113-118. Epub 2017 Dec 19.
    Department of Chemistry and Biotechnology, School of Science, Tallinn University of Technology, Akadeemia tee 15, Tallinn 12618, Estonia. Electronic address:
    Deoxynucleoside 5-monophosphate N-glycosidase or DNPH1 (former name Rcl) is a nucleotide hydrolase whose expression in mammalian cancer tissues has been associated with its tumorigenic potential. Therefore, the enzyme has been studied principally in rat and human models. We found the corresponding gene also in the freshwater sponge Ephydatia muelleri, an animal phylogenetically very distant from mammals. Read More

    Toluidine blue O modifies hippocampal amyloid pathology in a transgenic mouse model of Alzheimer's disease.
    Biochimie 2018 Mar 14;146:105-112. Epub 2017 Dec 14.
    Department of Biochemistry, School of Pharmacy, Hacettepe University, 06100, Ankara, Turkey. Electronic address:
    Recently, we have demonstrated that toluidine blue O (TBO), a phenothiazine dye, shows inhibitory effects on both cholinesterases and amyloid pathology in Alzheimer's disease (AD) cellular model. In the present study, we aimed to determine the effects of TBO (in a purity of 85%) on amyloid and tau pathologies in a triple transgenic mouse model of AD (3xTg-AD). Beginning at 7. Read More

    Chicken GRIFIN: Structural characterization in crystals and in solution.
    Biochimie 2018 Mar 15;146:127-138. Epub 2017 Dec 15.
    Institute of Physiological Chemistry, Faculty of Veterinary Medicine, Ludwig-Maximilians-University, Veterinärstr. 13, 80539 Munich, Germany. Electronic address:
    Despite its natural abundance in lenses of vertebrates the physiological function(s) of the galectin-related inter-fiber protein (GRIFIN) is (are) still unclear. The same holds true for the significance of the unique interspecies (fish/birds vs mammals) variability in the capacity to bind lactose. In solution, ultracentrifugation and small angle X-ray scattering (at concentrations up to 9 mg/mL) characterize the protein as compact and stable homodimer without evidence for aggregation. Read More

    Secretory lipase from the human pathogen Leishmania major: Heterologous expression in the yeast Pichia pastoris and biochemical characterization.
    Biochimie 2018 Mar 13;146:119-126. Epub 2017 Dec 13.
    Laboratoire de Biochimie et de Génie Enzymatique des Lipases, Université de Sfax, ENIS Route de Soukra, Sfax, Tunisia. Electronic address:
    Leishmaniasis is a parasitic reticuloendotheliosis whose pathogen is a zooflagellate belonging to the genus Leishmania transmitted by the bite of an infected phlebotome. Recently, a unique secretory lipase from the human pathogen Leishmania donovani Ldlip3 has been identified and characterized. This lipase has a high identity with a putative triacylglycerol lipase of Leishmania major (Lmlip2). Read More

    Aptamers as potential therapeutic agents for ovarian cancer.
    Biochimie 2018 Feb 7;145:34-44. Epub 2017 Dec 7.
    School of Medicine and Centre for Molecular and Medical Research, Deakin University, 75 Pigdons Road, Waurn Ponds, Victoria 3216, Australia. Electronic address:
    Current therapy for ovarian cancer typically involves indiscriminate chemotherapies that can have severe off target effects on healthy tissue and are still plagued by aggressive recurrence. Recent shifts towards targeted therapies offer the possibility of circumventing the obstacles experienced by these traditional treatments. While antibodies are the pioneering agents in targeted therapies, clinical experience has demonstrated that their antitumor efficacy is limited due to their high immunogenicity, large molecular size, and costly and laborious production. Read More

    Nobiletin induces brown adipocyte-like phenotype and ameliorates stress in 3T3-L1 adipocytes.
    Biochimie 2018 Mar 5;146:97-104. Epub 2017 Dec 5.
    Department of Biotechnology, Daegu University, Kyungsan, Kyungbuk, 38543, Republic of Korea. Electronic address:
    Browning of white adipocytes (beiging) is an attractive therapeutic strategy against obesity and its associated metabolic complications. Nobiletin (NOB) is a polymethoxylated flavone present in citrus fruits and has been reported to have anti-obesity effects. Here, we report that nobiletin exerts dual modulatory effects on adipocytes via induction of browning in 3T3-L1 white adipocytes and amelioration of stress in adipocytes. Read More

    Multi-targeted effects of G4-aptamers and their antiproliferative activity against cancer cells.
    Biochimie 2018 Feb 5;145:163-173. Epub 2017 Dec 5.
    N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health, Moscow, Russian Federation.
    We selected and investigated nine G-quadruplex (G4)-forming aptamers originally designed against different proteins involved in the regulation of cellular proliferation (STAT3, nucleolin, TOP1, SP1, VEGF, and SHP-2) and considered to be potential anticancer agents. We showed that under physiological conditions all the aptamers form stable G4s of different topology. G4 aptamers designed against STAT3, nucleolin and SP1 inhibit STAT3 transcriptional activity in human breast adenocarcinoma MCF-7 cells, and all the studied aptamers inhibit TOP1-mediated relaxation of supercoiled plasmid DNA. Read More

    Aptamers targeting cell surface proteins.
    Biochimie 2018 Feb 2;145:63-72. Epub 2017 Dec 2.
    Division of RNA Medical Science, The Institute of Medical Science, The University of Tokyo, 4-6-1, Shirokanedai, Minato-ku, Tokyo, Japan. Electronic address:
    High affinity binders targeting specific cell surface proteins are vital for development of basic and applied biosciences. However, despite sustained efforts to generate such binders by chemicals and antibodies, there are still many cell surface proteins that lack high affinity binders. Nucleic acid aptamers have potential as binding molecules for cell surface proteins, because they form distinct structures that have high affinity and specificity for a wide range of targets. Read More

    Degradation of tropoelastin and skin elastin by neprilysin.
    Biochimie 2018 Mar 29;146:73-78. Epub 2017 Nov 29.
    Institute of Pharmacy, Faculty of Natural Sciences I, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany; Department of Pharmacy, University of Copenhagen, Copenhagen, Denmark. Electronic address:
    Neprilysin is also known as skin fibroblast-derived elastase, and its up-regulation during aging is associated with impairments of the elastic fiber network, loss of skin elasticity and wrinkle formation. However, information on its elastase activity is still limited. The aim of this study was to investigate the degradation of fibrillar skin elastin by neprilysin and the influence of the donor's age on the degradation process using mass spectrometry and bioinformatics approaches. Read More

    The binding efficiency of RPA to telomeric G-strands folded into contiguous G-quadruplexes is independent of the number of G4 units.
    Biochimie 2018 Mar 29;146:68-72. Epub 2017 Nov 29.
    Structure et Instabilité des Génomes, Sorbonne Universités, Muséum National d'Histoire Naturelle, CNRS UMR 7196, INSERM U1154, 43 rue Cuvier, F-75005, Paris, France; Sorbonne Universités, UPMC Univ Paris 06, UFR927, F-75005, Paris, France. Electronic address:
    Replication protein A (RPA) is a single-stranded DNA binding protein involved in replication and in telomere maintenance. During telomere replication, G-quadruplexes (G4) can accumulate on the lagging strand template and need to be resolved. It has been shown that human RPA is able to unfold a single G4. Read More

    Serine 51 residue of Citrobacter freundii tyrosine phenol-lyase assists in C-α-proton abstraction and transfer in the reaction with substrate.
    Biochimie 2017 Nov 25. Epub 2017 Nov 25.
    Nesmeyanov Institute of Organoelement Compounds, Russian Academy of Sciences, Moscow 119991, Vavilov Street 28, Russia. Electronic address:
    In the spatial structure of tyrosine phenol-lyase, the Ser51 residue is located in the active site of the enzyme. The replacement of Ser51 with Ala by site-directed mutagenesis led to a decrease of the k/Kparameter for reactions with l-tyrosine and 3-fluoro-l-tyrosine by three orders of magnitude, compared to wild type enzyme. For the elimination reactions of S-alkylcysteines, the values of k/Kdecreased by an average of two orders of magnitude. Read More

    Insulin resistance in 3T3-L1 adipocytes by TNF-α is improved by punicic acid through upregulation of insulin signalling pathway and endocrine function, and downregulation of proinflammatory cytokines.
    Biochimie 2018 Mar 26;146:79-86. Epub 2017 Nov 26.
    Cell Culture and Molecular Mechanism Lab, Agroprocessing and Technology Division, CSIR-National Institute for Interdisciplinary Science and Technology (NIIST), Thiruvananthapuram, Kerala, 695019, India. Electronic address:
    Insulin resistance (IR) has become a major threat to public health due to its role in metabolic syndrome. Inflammation associated with IR is an interesting area of biomedical research in recent years and is expected to affect insulin signalling pathway via downregulating glucose transporters. In the present study, we evaluate the potential of punicic acid (PA), a nutraceutical found in pomegranate seed oil, against TNF-α induced alteration in 3T3-L1 adipocytes on glucose metabolism, endocrine function and inflammation. Read More

    Aptamers in Bordeaux 2017: An exceptional "millésime".
    Biochimie 2018 Feb 24;145:2-7. Epub 2017 Nov 24.
    ARNA Laboratory, University of Bordeaux, 33076 Bordeaux, France.
    About 150 participants attended the symposium organised at the Palais de la Bourse in Bordeaux, France on September 22-23, 2017. Thirty speakers from all over the world delivered lectures covering selection processes, aptamer chemistry and innovative applications of these powerful tools that display major advantages over antibodies. Beyond the remarkable science presented, lively discussion and fruitful exchange between participants made this meeting a great success. Read More

    DNA mismatch repair proteins MLH1 and PMS2 can be imported to the nucleus by a classical nuclear import pathway.
    Biochimie 2018 Mar 24;146:87-96. Epub 2017 Nov 24.
    Departamento de Física e Biofísica, Instituto de Biociências, Universidade Estadual Paulista, Botucatu, SP, Brazil. Electronic address:
    MLH1 and PMS2 proteins form the MutLα heterodimer, which plays a major role in DNA mismatch repair (MMR) in humans. Mutations in MMR-related proteins are associated with cancer, especially with colon cancer. The N-terminal region of MutLα comprises the N-termini of PMS2 and MLH1 and, similarly, the C-terminal region of MutLα is composed by the C-termini of PMS2 and MLH1, and the two are connected by linker region. Read More

    VDR independent induction of acid-sphingomyelinase by 1,23(OH)Din gastric cancer cells: Impact on apoptosis and cell morphology.
    Biochimie 2018 Mar 20;146:35-42. Epub 2017 Nov 20.
    Department of Pharmaceutical Science, University of Perugia, Italy. Electronic address:
    1 alpha,25-dihydroxyvitamin D(1,23(OH)D) is known to play a dual role in cancer, by promoting or inhibiting carcinogenesis via 1,23(OH)Dreceptor (VDR) and phosphatase and tensin homolog deleted on chromosome 10 (PTEN). Fok I polymorphism of VDR may indirectly influence the receptor levels through autoregulation. The involvement of neutral sphingomyelinase in the non-classic VDR-mediated genomic pathway response to 1,23(OH)Dtreatment has been reported. Read More

    1,25(OH)Dinduced apoptosis of human hepatocellular carcinoma cells in vitro and inhibited their growth in a nude mouse xenograft model by regulating histone deacetylase 2.
    Biochimie 2018 Mar 20;146:28-34. Epub 2017 Nov 20.
    Medical Laboratory, Guizhou Medical University, Guizhou, China. Electronic address:
    The aim of this study was to evaluate the effects of 1,25(OH)Don the apoptosis of human hepatocellular carcinoma (HCC) cells. The effects of 1,25(OH)Don the proliferation and apoptosis of human HCC cells by regulating histone deacetylase 2 (HDAC2) were assessed by MTT assay and flow cytometry. The effects of 1,25(OH)Don the expressions of related proteins in the apoptosis pathway by regulating HDAC2 as well as the mechanism were studied by Western blot and quantitative real-time PCR. Read More

    Probing the interaction of copper cofactor and azachalcone substrate with G-quadruplex of DNA based Diels-Alderase by site-specific fluorescence quenching titration.
    Biochimie 2018 Mar 17;146:20-27. Epub 2017 Nov 17.
    State Key Laboratory of Catalysis, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China. Electronic address:
    DNAzymes have been widely used in biosensors, asymmetric synthesis and pharmaceuticals. Typically, metal cofactor and substrate interact with DNA by supramolecular interactions in DNAzyme based asymmetric catalysis. However, binding positions of cofactor and substrate with DNA scaffold are not well understood, which is an obstacle to reveal the assembly and catalysis mechanisms of DNAzyme. Read More

    Heat stress in the heart and muscle of the Antarctic fishes Notothenia rossii and Notothenia coriiceps: Carbohydrate metabolism and antioxidant defence.
    Biochimie 2018 Mar 16;146:43-55. Epub 2017 Nov 16.
    Department of Cell Biology, Federal University of Paraná, Curitiba, Paraná, Brazil. Electronic address:
    Carbohydrate metabolism and the antioxidant defence system of heart and muscle of the Antarctic notothenioids Notothenia rossii and Notothenia coriiceps were evaluated in response to heat stress (8 °C) over 144 h. N. rossii heart exhibited decreased glycolysis and aerobic metabolism after up to 12 h of exposure to 8 °C, and anaerobiosis was inhibited within 24 h. Read More

    Targeting of NT5E by miR-30b and miR-340 attenuates proliferation, invasion and migration of gallbladder carcinoma.
    Biochimie 2018 Mar 17;146:56-67. Epub 2017 Nov 17.
    Department of General Surgery, Affiliated Hospital, Logistics University of People's Armed Police Force, Tianjin 300162, China. Electronic address:
    MicroRNAs (miRNAs) have been closely associated with the proliferation, invasion and migration of various cancers, including gallbladder carcinoma (GBC). Previous studies have revealed dysregulation of miR-30b and miR-340 in many types of cancer. However, the role of miR-30b and miR-340 in the development and progression of GBC remains unclear. Read More

    Functional characterization of a serine protease inhibitor modulated in the infection of the Aedes aegypti with dengue virus.
    Biochimie 2018 Jan 11;144:160-168. Epub 2017 Nov 11.
    Departamento de Bioquímica, Escola Paulista de Medicina, Universidade Federal de São Paulo, Rua 3 de Maio 100, 04044-020, São Paulo, SP, Brazil. Electronic address:
    During feeding with blood meal, female Aedes aegypti can transmit infectious agents, such as dengue, yellow fever, chikungunya and Zika viruses. Dengue virus causes human mortality in tropical regions of the world, and there is no specific treatment or vaccine with maximum efficiency being used for these infections. In the vector-virus interaction, the production of several molecules is modulated by both mosquitoes and invading agents. Read More

    Application of mixed peptide arrays to study combinatorial readout of chromatin modifications.
    Biochimie 2018 Mar 11;146:14-19. Epub 2017 Nov 11.
    Department of Biochemistry, Institute of Biochemistry and Technical Biochemistry, University Stuttgart, Allmandring 31, 70569 Stuttgart, Germany. Electronic address:
    The N-terminal tails of histone proteins are massively decorated with post-translational modifications (PTMs), which play important roles in the regulation of gene expression. Several highly conserved chromatin interacting proteins can bind to histone modifications in a sequence and modification specific manner employing specific reading domains. These proteins often contain several reading domains, which can cooperate in the readout of different PTMs. Read More

    Structural features of the interaction of the 3'-untranslated region of mRNA containing exosomal RNA-specific motifs with YB-1, a potential mediator of mRNA sorting.
    Biochimie 2018 Jan 11;144:134-143. Epub 2017 Nov 11.
    Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Prospekt Lavrentieva 8, Novosibirsk, 630090, Russia; Department of Molecular Biology, Novosibirsk State University, Novosibirsk, 630090, Russia. Electronic address:
    We have previously shown that YB-1 is the only protein of the HEK293 cell cytoplasmic (S100) extract that specifically interacts with RNA hairpins each containing one of the motifs ACCAGCCU (1), CAGUGAGC (2) and UAAUCCCA (3), which had been identified as often found in exosomal RNA and proposed as potential cis-acting elements targeting RNAs into exosomes. Here we explored the interactions of YB-1 with a fragment of the 3'-untranslated region (UTR) of septin 14 mRNA (SEPT14 RNA), which contains all three motifs. We demonstrated the occurrence of YB-1 among proteins pulled down from the HEK293 S100 extract using biotinylated SEPT14 RNA. Read More

    Fluorescent light-up acridine orange derivatives bind and stabilize KRAS-22RT G-quadruplex.
    Biochimie 2018 Jan 10;144:144-152. Epub 2017 Nov 10.
    CICS-UBI - Centro de Investigação em Ciências da Saúde, Universidade da Beira Interior, Av. Infante D. Henrique, 6200-506, Covilhã, Portugal. Electronic address:
    KRAS is often found mutated in lethal cancers and should be an important target for anticancer drug development. However, no effective inhibitor has been reported so far, prompting the scientific community to describe the RAS proteins as nearly "undruggable". Recent approaches developed to modulate KRAS protein expression comprises the targeting of G-quadruplex (G4) structures formed within the nuclease hypersensitive element of KRAS promoter region, by designing small and specific ligands to stabilize the tertiary fold and reduce gene expression. Read More

    Upregulation of aquaporin 3 expression by diterpenoids in Euphorbia pekinensis is associated with activation of the NF-κB signaling pathway in the co-culture system of HT-29 and RAW 264.7 cells.
    Biochimie 2018 Jan 9;144:153-159. Epub 2017 Nov 9.
    School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. Electronic address:
    This study was designed to evaluate the toxic effects of diterpenoids separated from the roots of Euphorbia pekinensis, a type of widely used traditional Chinese medicine. This herb has intestinal toxicity associated with its complex diterpenoids. In this study, the diterpenoids (pekinenin A, pekinenin C, pekinenin F, pekinenin G, yuexiandajisu A, (-)-(1S)-15-hydroxy-18-carboxycembrene) elevated the expression of interleukin 1 beta and tumor necrosis factor alpha in a dose-dependent manner at doses of 6. Read More

    Comprehensive identification of proteins binding to RNA G-quadruplex motifs in the 5' UTR of tumor-associated mRNAs.
    Biochimie 2018 Jan 10;144:169-184. Epub 2017 Nov 10.
    Department of Applied Biochemistry, Institute of Biotechnology, TIB 4/3-2, Technische Universität Berlin, Gustav-Meyer-Allee 25, 13355, Berlin, Germany. Electronic address:
    G-quadruplex structures in the 5' UTR of mRNAs are widely considered to suppress translation without affecting transcription. The current study describes the comprehensive analysis of proteins binding to four different G-quadruplex motifs located in mRNAs of the cancer-related genes Bcl-2, NRAS, MMP16, and ARPC2. Following metabolic labeling (Stable Isotope Labeling with Amino acids in Cell culture, SILAC) of proteins in the human cell line HEK293, G-quadruplex binding proteins were enriched by pull-down assays and identified by LC-orbitrap mass spectrometry. Read More

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