8,163 results match your criteria Biochimie[Journal]


Corrigendum to "Regulation of β-catenin stabilization in human platelets" [Biochimie 95 (6) (2013) 1252-1257].

Biochimie 2019 Feb 18. Epub 2019 Feb 18.

Department of Biochemistry, Institute of Medical Sciences, Banaras Hindu University, Varanasi, 221005, UP, India. Electronic address:

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http://dx.doi.org/10.1016/j.biochi.2019.02.001DOI Listing
February 2019

A minimum set of stable blocks for rational design of polypeptide chains.

Biochimie 2019 Feb 18. Epub 2019 Feb 18.

Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilov St. 32, 119991 Moscow, Russia.

The aim of this work was to find a minimal set of structurally stable pentapeptides, which allows forming a polypeptide chain of a required 3D structure. To search for factors that ensure structural stability of the pentapeptide, we generated peptide sequences with no more than three functional groups, based on the alanine pentapeptide AAAAA. We analyzed 44,860 structures of peptides by the molecular dynamics method and found that 1,225 pentapeptides over 80% of the simulation time were in a stable conformation. Read More

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http://dx.doi.org/10.1016/j.biochi.2019.02.006DOI Listing
February 2019

Microcin J25 inhibits ubiquinol oxidase activity of purified cytochrome bd-I from Escherichia coli.

Biochimie 2019 Feb 18. Epub 2019 Feb 18.

Instituto Superior de Investigaciones Biológicas (INSIBIO, CONICET-UNT) e Instituto de Química Biológica "Dr. Bernabé Bloj", Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, Chacabuco 461, San Miguel de Tucumán, Argentina. Electronic address:

Microcin J25 (MccJ25), an antimicrobial peptide, targets the respiratory chain but the exact mechanism by which it does so remains unclear. Here, we reveal that MccJ25 is able to inhibit the enzymatic activity of the isolated cytochrome bd-I from E. coli and induces at the same time production of reactive oxygen species. Read More

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http://dx.doi.org/10.1016/j.biochi.2019.02.007DOI Listing
February 2019

Cross-talk between the RcsCDB and RstAB systems to control STM1485 gene expression in Salmonella Typhimurium during acid-resistance response.

Biochimie 2019 Feb 11;160:46-54. Epub 2019 Feb 11.

Instituto Superior de Investigaciones Biológicas (INSIBIO), CONICET-UNT, and Instituto de Química Biológica "Dr. Bernabé Bloj", Facultad de Bioquímica, Química y Farmacia, UNT, Chacabuco 461, T4000ILI, San Miguel de Tucumán, Tucumán, Argentina. Electronic address:

Bacterial survive and respond to adverse changes in the environment by regulating gene transcription through two-component regulatory systems. In Salmonella Typhimurium the STM1485 gene expression is induced under low pH (4.5) during replication inside the epithelial host cell, but it is not involved in sensing or resisting to this condition. Read More

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http://dx.doi.org/10.1016/j.biochi.2019.02.005DOI Listing
February 2019

Morphine, but not methadone, inhibits microsomal prostaglandin E synthase-1 and prostaglandin-endoperoxide synthase 2 in lipopolysaccharide-stimulated horse synoviocytes.

Biochimie 2019 Feb 11;160:28-33. Epub 2019 Feb 11.

Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering, University of São Paulo, Pirassununga, SP, Brazil. Electronic address:

Osteoarthritis (OA) is one of the main locomotor disorders in horses. Although nonsteroidal anti-inflammatory drugs are the first-line treatment for OA, opioids could also be used. In previous studies, opioids showed promising anti-inflammatory and analgesic effects. Read More

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http://dx.doi.org/10.1016/j.biochi.2019.02.004DOI Listing
February 2019

The C7-aminomethylpyrrolidine group rescues the activity of a thio-fluoroquinolone.

Biochimie 2019 Feb 11;160:24-27. Epub 2019 Feb 11.

Department of Pharmacology, University of Minnesota Medical School, Minneapolis, MN, 55455, USA. Electronic address:

A Mg-water bridge between the C-3, C-4 diketo moiety of fluoroquinolones and the conserved amino acid residues in the GyrA/ParC subunit is critical for the binding of a fluoroquinolone to a topoisomerase-DNA covalent complex. The fluoroquinolone UING-5-249 (249) can bind to the GyrB subunit through its C7-aminomethylpyrrolidine group. This interaction is responsible for enhanced activities of 249 against the wild type and quinolone-resistant mutant topoisomerases. Read More

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http://dx.doi.org/10.1016/j.biochi.2019.02.002DOI Listing
February 2019

Thyroid peroxidase as a dual active site enzyme: Focus on biosynthesis, hormonogenesis and thyroid disorders of autoimmunity and cancer.

Biochimie 2019 Feb 10;160:34-45. Epub 2019 Feb 10.

King's College London, London, UK. Electronic address:

Thyroid peroxidase (TPO) is the key enzyme involved in thyroid hormone synthesis. Autoantibodies to TPO (TPOAbs) are a hallmark of autoimmune thyroid disease (AITD). Here, we highlight recent progress over several years in understanding TPO biochemistry and function in various pathologies. Read More

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http://dx.doi.org/10.1016/j.biochi.2019.02.003DOI Listing
February 2019
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The molecular link between tyrosol binding to tri6 transcriptional regulator and downregulation of trichothecene biosynthesis.

Biochimie 2019 Feb 4;160:14-23. Epub 2019 Feb 4.

Department of Biotechnology, Babasaheb Bhimrao Ambedkar University, Vidya Vihar, Raebareli Road, Lucknow, Uttar Pradesh, 226025, India. Electronic address:

Tri cluster is responsible for the biosynthesis of trichothecenes in Trichoderma spp. Tri6 gene present within the cluster encodes for a transcriptional regulator and is vital for the expression of all other tri genes of the cluster. Tri6 encodes for a 218-amino-acid residues long protein which contains three zinc finger motifs. Read More

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http://dx.doi.org/10.1016/j.biochi.2019.01.021DOI Listing
February 2019
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Role of epoxy-fatty acids and epoxide hydrolases in the pathology of neuro-inflammation.

Biochimie 2019 Feb 1. Epub 2019 Feb 1.

Department of Entomology and Nematology, UCD Comprehensive Cancer Center, University of California Davis, One Shields Avenue, Davis, CA, 95616, USA. Electronic address:

Neuroinflammation is a physiologic response aimed at protecting the central nervous system during injury. However, unresolved and chronic neuroinflammation can lead to long term damage and eventually neurologic disease including Parkinson's disease, Alzheimer's disease and dementia. Recently, enhancing the concentration of epoxyeicosatrienoic acids (EETs) through blocking their hydrolytic degradation by soluble epoxide hydrolase (sEH) has been applied towards reducing the long-term damage associated with central neurologic insults. Read More

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http://dx.doi.org/10.1016/j.biochi.2019.01.020DOI Listing
February 2019
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The n-3 docosapentaenoic acid (DPA): a new player in the n-3 long chain polyunsaturated fatty acid family.

Biochimie 2019 Feb 1. Epub 2019 Feb 1.

Laboratory of Biochemistry and Human Nutrition, Agrocampus Ouest, Rennes, F-35042, France. Electronic address:

The n-3 docosapentaenoic acid (n-3 DPA) is less studied n-3 long-chain polyunsaturated fatty acid (LCPUFA), compared to its counterparts eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). Present in food sources in non-negligible quantities, as well as in human milk, dietary n-3 DPA is of current interest both for its ability to increase EPA and DHA tissue status and for its specific or shared biological effects. Indeed, some evidence showed that dietary n-3 DPA is a source of EPA and slightly DHA in the major metabolic organs. Read More

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http://dx.doi.org/10.1016/j.biochi.2019.01.022DOI Listing
February 2019

Foreword.

Biochimie 2018 02 22;145. Epub 2017 Dec 22.

The University of Tokyo, Division of RNA Medical Science, Institute of Medical Science, 4-6-1 Shirokanedai, Minato-ku, Tokyo, 108-8639 Japan. Electronic address:

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http://dx.doi.org/10.1016/j.biochi.2017.12.009DOI Listing
February 2018

Elucidation of the mechanism of disulfide exchange between staphylococcal thioredoxin2 and thioredoxin reductase2: A structural insight.

Biochimie 2019 Jan 31;160:1-13. Epub 2019 Jan 31.

Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur, 721302, India. Electronic address:

The redox homeostasis of cytoplasm is maintained by a series of disulfide exchange reactions mediated by proteins belonging to the thioredoxin superfamily. Thioredoxin and thioredoxin reductase, being the major members of the family, play a key role in oxidative stress response of Staphylococcus aureus. In this report, we have identified and characterised an active thioredoxin system of the mentioned pathogen. Read More

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http://dx.doi.org/10.1016/j.biochi.2019.01.019DOI Listing
January 2019
2.963 Impact Factor

Development of conformational antibodies targeting Cripto-1 with neutralizing effects in vitro.

Biochimie 2019 Mar 28;158:246-256. Epub 2019 Jan 28.

Institute of Biostructure and Bioimaging, National Research Council (IBB-CNR), Naples, Italy. Electronic address:

Human Cripto-1 (Cripto-1), the founding member of the EGF-CFC superfamily, is a key regulator of many processes during embryonic development and oncogenesis. Cripto-1 is barely present or even absent in normal adult tissues while it is aberrantly re-expressed in various tumors. Blockade of the CFC domain-mediated Cripto-1 functions is acknowledged as a promising therapeutic intervention point to inhibit the tumorigenic activity of the protein. Read More

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http://dx.doi.org/10.1016/j.biochi.2019.01.016DOI Listing
March 2019
1 Read

Emergence of membrane sphingolipids as a potential therapeutic target.

Biochimie 2019 Mar 28;158:257-264. Epub 2019 Jan 28.

State Key Laboratory of Biocontrol, Guangdong Key Laboratory of Plant Resources, School of Life Science, Sun Yat-sen University, Guangzhou, 510275, China. Electronic address:

Background: Though sphingolipids are ubiquitously present in eukaryotic cells, but until the last decade, they were merely considered as a structural component of the plasma membrane with limited function. However, over the last decade, numerous functions have been ascribed to sphingolipids after the seminal discoveries on the bioactivities of several sphingolipids.

Scope Of Review: Sphingolipids are now well-recognized signals for fundamental cellular processes. Read More

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http://dx.doi.org/10.1016/j.biochi.2019.01.018DOI Listing

Structure function relationships in three lipids A from the Ralstonia genus rising in obese patients.

Biochimie 2019 Jan 28. Epub 2019 Jan 28.

I2BC, Centre National de la Recherche Scientifique, Université de Paris-Sud, Université de Paris-Saclay, Orsay, France; LPS-BioSciences, I2BC, Bâtiment 409, Université de Paris-Sud, Orsay, France. Electronic address:

The identification of a functional molecular moiety relating the lipopolysaccharides (LPSs) to their capacity to induce inflammation-mediated metabolic diseases needed to be performed. We previously described a proportional increase in the relative abundance of the 16 SrDNA bacterial gene from the genus Ralstonia, within the microbiota from the adipose tissue stroma vascular fraction of obese patients, suggesting a causal role of the bacteria. Therefore, we first characterized the structures of the lipids A, the inflammatory inducing moieties of LPSs, of three Ralstonia species: Ralstonia eutropha, R. Read More

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http://dx.doi.org/10.1016/j.biochi.2019.01.015DOI Listing
January 2019
2 Reads

New pieces to the carbon metabolism puzzle of Nitrosomonas europaea: Kinetic characterization of glyceraldehyde-3 phosphate and succinate semialdehyde dehydrogenases.

Biochimie 2019 Mar 26;158:238-245. Epub 2019 Jan 26.

Laboratorio de Enzimología Molecular, Instituto de Agrobiotecnología del Litoral (IAL, CONICET-UNL) & FBCB, Centro Científico Tecnológico CONICET Santa Fe, Santa Fe, Argentina; Instituto Pasteur de Montevideo, Montevideo, Uruguay. Electronic address:

Nitrosomonas europaea is a chemolithotroph that obtains energy through the oxidation of ammonia to hydroxylamine while assimilates atmospheric CO to cover the cell carbon demands for growth. This microorganism plays a relevant role in the nitrogen biogeochemical cycle on Earth but its carbon metabolism remains poorly characterized. Based on sequence homology, we identified two genes (cbbG and gabD) coding for redox enzymes in N. Read More

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http://dx.doi.org/10.1016/j.biochi.2019.01.013DOI Listing

N-3 polyunsaturated fatty acids: An innovative strategy against obesity and related metabolic disorders, intestinal alteration and gut microbiota dysbiosis.

Biochimie 2019 Jan 25. Epub 2019 Jan 25.

Université de Bourgogne Franche-Comté, UFR Sciences de la Vie, de la Terre et de l'Environnement, Lipides Nutrition Cancer UMR UMR1231, 6 Boulevard Gabriel, 21000, Dijon, France; INSERM, Lipides Nutrition Cancer UMR1231, 21000, Dijon, France; LipSTIC LabEx, Fondation de Coopération Scientifique Bourgogne-Franche Comté, 21000, Dijon, France. Electronic address:

Obesity is now widely recognized to be associated with low-grade systemic inflammation. It has been shown that high-fat feeding modulates gut microbiota which strongly increased intestinal permeability leading to lipopolysaccharide absorption causing metabolic endotoxemia that triggers inflammation and metabolic disorders. N-3 polyunsaturated fatty acids (PUFAs) have been shown associated with anti-obesity properties, but results still remain heterogeneous and very few studies underlined the metabolic pathways involved. Read More

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http://dx.doi.org/10.1016/j.biochi.2019.01.017DOI Listing
January 2019
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Dimers of serotonin receptors: Impact on ligand affinity and signaling.

Biochimie 2019 Jan 24. Epub 2019 Jan 24.

INSERM UMR-S839, S1270, Paris, 75005, France; Sorbonne Université, Paris, 75005, France; Institut du Fer à Moulin, Paris, 75005, France.

Membrane receptors often form complexes with other membrane proteins that directly interact with different effectors of the signal transduction machinery. G-protein-coupled receptors (GPCRs) were for long time considered as single pharmacological entities. However, evidence for oligomerization appeared for various classes and subtypes of GPCRs. Read More

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http://dx.doi.org/10.1016/j.biochi.2019.01.009DOI Listing
January 2019
2.963 Impact Factor

An aptamer based thermofluorimetric assay for ethanolamine.

Biochimie 2019 Mar 25;158:233-237. Epub 2019 Jan 25.

Furtwangen University, Institute of Precision Medicine, Jakob-Kienzle-Straße 17, 78054, Villingen-Schwenningen, Germany; Fraunhofer Institute IZI, Leipzig, EXIM Department, Schillingallee 68, D-18057, Rostock, Germany. Electronic address:

There is a great need for fast, simple and precise diagnostic assays capable of direct quantification of biomarkers in complex biological matrices. Yet, the commonly used techniques such as ELISA/Immunoassays are tedious and involve various steps e.g. Read More

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http://dx.doi.org/10.1016/j.biochi.2019.01.014DOI Listing
March 2019
1 Read

Kinetic mechanism of Enterococcus faeciumd-aspartate ligase.

Biochimie 2019 Mar 22;158:217-223. Epub 2019 Jan 22.

Faculty of Pharmacy, University of Ljubljana, Aškerčeva 7, 1000, Ljubljana, Slovenia. Electronic address:

Enterococcus faeciumd-aspartate ligase (Asl) is a peptide bond-forming enzyme that is involved in the peptidoglycan assembly pathway. It catalyzes the ATP-dependent ligation of the β-carboxylate of D-Asp to the ε-amino group of L-Lys in the nucleotide precursor UDP- MurNAc-pentapeptide. The enzyme is of interest as a target of new, potential, narrow-spectrum antibiotics directed against multiresistant E. Read More

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http://dx.doi.org/10.1016/j.biochi.2019.01.012DOI Listing
March 2019
2 Reads

Hydrogen sulfide preconditioning could ameliorate reperfusion associated injury in diabetic cardiomyopathy rat heart through preservation of mitochondria.

Biochimie 2019 Mar 22;158:208-216. Epub 2019 Jan 22.

Vascular Biology Lab, School of Chemical and Biotechnology, SASTRA University, Thanjavur, 613401, India. Electronic address:

Evidence suggests that hydrogen sulfide precondition (HIPC) is an effective protocol in the management of ischemia reperfusion (I/R) by attenuating free radical and calcium overload in mitochondria. However the efficacy of HIPC is largely unknown in diabetic cardiomyopathy (DCM) hearts subjected to I/R procedure. Male Wistar rats were randomly divided into three groups: i) normal, ii) diabetes mellitus (DM), and iii) diabetic cardiomyopathy (DCM). Read More

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http://dx.doi.org/10.1016/j.biochi.2019.01.011DOI Listing
March 2019
1 Read

Vasopressin receptors in islets enhance glucose tolerance, pancreatic beta-cell secretory function, proliferation and survival.

Biochimie 2019 Mar 21;158:191-198. Epub 2019 Jan 21.

SAAD Centre for Pharmacy and Diabetes, University of Ulster, Coleraine, Northern Ireland, UK.

Arginine vasopressin (AVP), a peptide secreted from the posterior pituitary, is chiefly regarded as a hormone involved in the regulation of body fluid balance and osmolality. However, recent evidence has revealed that posterior pituitary hormones can exert important actions on endocrine pancreatic function. In the present study, the presence of AVP receptors, namely Avpr1a (V1a), Avpr1b (V1b) and Avpr2 (V2) was demonstrated in murine islets as well as rodent BRIN BD11 and human 1. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S03009084193001
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http://dx.doi.org/10.1016/j.biochi.2019.01.008DOI Listing
March 2019
5 Reads

Properties of l-amino acid deaminase: En route to optimize bioconversion reactions.

Biochimie 2019 Mar 20;158:199-207. Epub 2019 Jan 20.

Dipartimento di Biotecnologie e Scienze della Vita, Università degli Studi dell'Insubria, via J. H. Dunant 3, 21100, Varese, Italy. Electronic address:

Interest is rising in the agrochemical and pharmaceutical industries concerning the use of enantiomerically pure amino acids. l-Amino acids are easily produced by deracemization of D,L-mixtures or by stereoinversion of d-amino acids, employing the flavoenzyme d-amino acid oxidase. On the other hand, the production of the D-enantiomers is hampered by the lack of a suitable enzyme with reversed stereoselectivity. Read More

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http://dx.doi.org/10.1016/j.biochi.2019.01.010DOI Listing
March 2019
1 Read
2.963 Impact Factor

Structural basis for the design of selective inhibitors for Schistosoma mansoni dihydroorotate dehydrogenase.

Biochimie 2019 Mar 19;158:180-190. Epub 2019 Jan 19.

Laboratório de Cristalografia de Proteínas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, 14040-903, Brazil.

Trematode worms of the genus Schistosoma are the causing agents of schistosomiasis, a parasitic disease responsible for a considerable economic and healthy burden worldwide. In the present work, the characterization of the enzyme dihydroorotate dehydrogenase from Schistosoma mansoni (SmDHODH) is presented. Our studies demonstrated that SmDHODH is a member of class 2 DHODHs and catalyzes the oxidation of dihydroorotate into orotate using quinone as an electron acceptor by employing a ping-pong mechanism of catalysis. Read More

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http://dx.doi.org/10.1016/j.biochi.2019.01.006DOI Listing
March 2019
2 Reads

M17 aminopeptidases diversify function by moderating their macromolecular assemblies and active site environment.

Biochimie 2019 Jan 14. Epub 2019 Jan 14.

Biomedicine Discovery Institute, Department of Microbiology, Monash University, Melbourne, VIC, 3800, Australia. Electronic address:

The family of M17 aminopeptidases (alias 'leucine aminopeptidases', M17-LAPs) utilize a highly conserved hexameric structure and a binuclear metal center to selectively remove N-terminal amino acids from short peptides. However, M17-LAPs are responsible for a wide variety of functions that are seemingly unrelated to proteolysis. Herein, we aimed to investigate the myriad of functions attributed to M17. Read More

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http://dx.doi.org/10.1016/j.biochi.2019.01.007DOI Listing
January 2019
1 Read

Biomarkers mapping of neuropathic pain in a nerve chronic constriction injury mice model.

Biochimie 2019 Mar 11;158:172-179. Epub 2019 Jan 11.

School of Biosciences and Veterinary Medicine, Italy.

Neuropathic pain is caused by a lesion or disease of the somatosensory nervous system and has a considerable impact on the quality of life. Neuropathic pain has a dynamic and complex aetiology and gives heterogeneous symptoms across patients; therefore, it represents an important clinical challenge. Current pharmacological treatment includes tricyclic antidepressant serotonin-noradrenaline uptake inhibitors such as duloxetine, pregabalin, and gabapentin. Read More

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http://dx.doi.org/10.1016/j.biochi.2019.01.005DOI Listing
March 2019
3 Reads

Oxalomalate reduces tumor progression in melanoma via ROS-dependent proapoptotic and antiangiogenic effects.

Biochimie 2019 Mar 9;158:165-171. Epub 2019 Jan 9.

School of Life Sciences and Biotechnology, BK21 Plus KNU Creative BioResearch Group, College of Natural Sciences, Kyungpook National University, Taegu, South Korea. Electronic address:

The potent cytotoxicity of reactive oxygen species (ROS) can cause various diseases, however, it may also serve as a powerful chemotherapeutic strategy capable of killing cancer cells. Oxalomalate (OMA, α-hydroxy-β-oxalosuccinic acid), a tricarboxylic acid intermediate, is a well-known competitive inhibitor of two classes of NADP-dependent isocitrate dehydrogenase (IDH) isoenzymes, which serve as the major antioxidants and redox regulators in the mitochondria and cytosol. In this study, we investigated the therapeutic effects of OMA in melanoma and elucidated the associated underlying mechanisms of action using in vitro and in vivo models. Read More

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http://dx.doi.org/10.1016/j.biochi.2019.01.004DOI Listing
March 2019
2 Reads

Molecular cloning and characterization of porcine Na⁺/K⁺-ATPase isoform α4.

Biochimie 2019 Mar 8;158:149-155. Epub 2019 Jan 8.

Department of Molecular Biology and Genetics, Aarhus University, C.F. Møllers Allé 3, DK-8000, Aarhus C, Denmark. Electronic address:

Na/K-ATPase is responsible for maintaining electrochemical gradients of Na and K, which is essential for a variety of cellular functions including neuronal activity. The α-subunit of the Na/K-ATPase is composed of four different polypeptides (α1-α4) encoded by different genes. Na,K-ATPase α4, encoded by the ATP1A4 gene, is expressed in testis and in male germ cells of humans, rats and mice. Read More

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http://dx.doi.org/10.1016/j.biochi.2019.01.003DOI Listing
March 2019
3 Reads

Osmolytes resist against harsh osmolarity: Something old something new.

Biochimie 2019 Mar 7;158:156-164. Epub 2019 Jan 7.

Kidney Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. Electronic address:

From the halophilic bacteria to human, cells have to survive under the stresses of harsh environments. Hyperosmotic stress is a process that triggers cell shrinkage, oxidative stress, DNA damage, and apoptosis and it potentially contributes to a number of human diseases. Remarkably, by high salts and organic solutes concentrations, a variety of organisms struggle with these conditions. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S03009084193000
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http://dx.doi.org/10.1016/j.biochi.2019.01.002DOI Listing
March 2019
8 Reads

FNDC5: A novel player in metabolism and metabolic syndrome.

Biochimie 2019 Mar 3;158:111-116. Epub 2019 Jan 3.

Cardiac Rehabilitation Program, Shanghai Xuhui Central Hospital/Zhongshan-Xuhui Hospital, Fudan University/Shanghai Clinical Research Center, Chinese Academy of Sciences, 966 Middle Huaihai Road, Shanghai 200031, China. Electronic address:

Half a decade ago, transmembrane protein fibronectin type III domain-containing protein 5 (FNDC5) was found to be cleaved as a novel myokine irisin, which burst into prominence for browning of white adipose tissue during exercise. However, FNDC5, the precursor of irisin, has been paid relatively little attention compared with irisin despite evidence that FNDC5 is associated with the metabolic syndrome, which accounts for one-fourth of the world's adult population and contributes to diabetes, cardiovascular disease and all-cause mortality. Besides N-terminal and C-terminal sequences, the FNDC5 protein contains an irisin domain and a short transmembrane region. Read More

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http://dx.doi.org/10.1016/j.biochi.2019.01.001DOI Listing
March 2019
2 Reads

The relationship between the placental serotonin pathway and fetal growth restriction.

Biochimie 2018 Dec 31. Epub 2018 Dec 31.

The Ritchie Centre, Hudson Institute of Medical Research, Clayton, Victoria, Australia; Department of Maternal-Fetal Medicine, Pregnancy Research Centre, Royal Women's Hospital, Parkville, Victoria, Australia; Department of Medicine, School of Clinical Sciences, Clayton, Victoria, Australia; Department of Obstetrics and Gynaecology, University of Melbourne, Royal Women's Hospital, Parkville, Victoria, Australia. Electronic address:

Fetal growth restriction (FGR) is a complex disorder of human pregnancy that leads to poor health outcomes in offspring. These range from immediate risks such as perinatal morbidity and stillbirths, to long-term complications including severe neurodevelopmental problems. Despite its relatively high global prevalence, the aetiology of FGR and its complications is not currently well understood. Read More

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http://dx.doi.org/10.1016/j.biochi.2018.12.016DOI Listing
December 2018
1 Read

Biochemical and structural insights into PLP fold type IV transaminase from Thermobaculum terrenum.

Biochimie 2019 Mar 29;158:130-138. Epub 2018 Dec 29.

Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Ave. 33, Bld. 2, 119071, Moscow, Russian Federation; Kurchatov Complex of NBICS-technologies, National Research Centre "Kurchatov Institute", Akad. Kurchatova Sqr 1, 123182, Moscow, Russian Federation.

The high catalytic efficiency of enzymes under reaction conditions is one of the main goals in biocatalysis. Despite the dramatic progress in the development of more efficient biocatalysts by protein design, the search for natural enzymes with useful properties remains a promising strategy. The pyridoxal 5'-phosphate (PLP)-dependent transaminases represent a group of industrially important enzymes due to their ability to stereoselectively transfer amino groups between diverse substrates; however, the complex mechanism of substrate recognition and conversion makes the design of transaminases a challenging task. Read More

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http://dx.doi.org/10.1016/j.biochi.2018.12.017DOI Listing
March 2019
3 Reads

Amelioration of sepsis-induced acute kidney injury through inhibition of inflammatory cytokines and oxidative stress in dendritic cells and neutrophils respectively in mice: Role of spleen tyrosine kinase signaling.

Biochimie 2019 Mar 29;158:102-110. Epub 2018 Dec 29.

Department of Pharmacology & Toxicology, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.

Sepsis often leads to complications such as acute kidney injury (AKI) which is reported to range from 30 to 50% in critically ill patients. Dendritic (DCs) and neutrophils play a decisive role in the advancement of AKI through release of inflammatory cytokines and reactive oxygen species (ROS) respectively. Both of these processes are assumed to be controlled by spleen tyrosine kinase (Syk) signaling in DCs and neutrophils. Read More

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http://dx.doi.org/10.1016/j.biochi.2018.12.014DOI Listing
March 2019
2 Reads

Cloning and expression of ATP N-glycosidase from the freshwater sponge Ephydatia muelleri.

Biochimie 2019 Mar 29;158:126-129. Epub 2018 Dec 29.

Department of Chemistry and Biotechnology, Division of Gene Technology, Tallinn University of Technology, Akadeemia tee 15, Tallinn, 12618, Estonia. Electronic address:

Previously we had discovered unusual enzymatic activity in the marine sponge Axinella polypoides, ATP N-glycosidase (Reintamm et al., 2003). We show here that the Ephydatia muelleri mRNA encoding protein with PNP_UDP_1 (phosphorylase superfamily) signature is the secreted ATP N-glycosidase. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S03009084183036
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http://dx.doi.org/10.1016/j.biochi.2018.12.018DOI Listing
March 2019
6 Reads

The human ribosome can interact with the abasic site in mRNA via a specific peptide of the uS3 protein located near the mRNA entry channel.

Biochimie 2019 Mar 27;158:117-125. Epub 2018 Dec 27.

Institute of Chemical Biology and Fundamental Medicine SB RAS, Novosibirsk, 630090, Russia; Novosibirsk State University, Novosibirsk, 630090, Russia. Electronic address:

The small subunit ribosomal protein uS3 is a critically important player in the ribosome-mRNA interactions during translation and has numerous functions not directly related to protein synthesis in eukaryotes. A peculiar feature of the human uS3 protein is the ability of its fragment 55-64 exposed on the 40S subunit surface near the mRNA entry channel to form cross-links with 3'-terminal dialdehyde derivatives of various unstructured RNAs and with abasic sites in single-stranded DNAs. Here we showed that the ability of the above uS3 fragment to cross-link to abasic sites in DNAs is inherent only in mature cytoplasmic 40S subunits, but not nuclear pre-40S particles, which implies that it may be relevant to the ribosome-mRNA interplay. Read More

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http://dx.doi.org/10.1016/j.biochi.2018.12.015DOI Listing
March 2019
1 Read

ORP2 interacts with phosphoinositides and controls the subcellular distribution of cholesterol.

Biochimie 2019 Mar 24;158:90-101. Epub 2018 Dec 24.

Minerva Foundation Institute for Medical Research, Biomedicum 2U, FI-00290, Helsinki, Finland; Department of Anatomy, Faculty of Medicine, FI-00014, University of Helsinki, Finland. Electronic address:

ORP2 is a sterol-binding protein with documented functions in lipid and glucose metabolism, Akt signaling, steroidogenesis, cell adhesion, migration and proliferation. Here we investigate the interactions of ORP2 with phosphoinositides (PIPs) by surface plasmon resonance (SPR), its affinity for cholesterol with a pull-down assay, and its capacity to transfer sterol in vitro. Moreover, we determine the effects of wild-type (wt) ORP2 and a mutant with attenuated PIP binding, ORP2(mHHK), on the subcellular distribution of cholesterol, and analyze the interaction of ORP2 with the related cholesterol transporter ORP1L. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S03009084183035
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http://dx.doi.org/10.1016/j.biochi.2018.12.013DOI Listing
March 2019
6 Reads

A comparative study of the complete lipopolysaccharide structures and biosynthesis loci of Bordetella avium, B. hinzii, and B. trematum.

Biochimie 2018 Dec 19. Epub 2018 Dec 19.

LPS-BioSciences, Bâtiment 409, Université de Paris-Sud, Paris-Saclay, F-91405, Orsay, France; Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université de Paris-Sud, Université Paris-Saclay, F-91405, Orsay, France. Electronic address:

A dozen species of human and animal pathogens have been described to date in the Bordetella genus, with the majority being respiratory tract pathogens. Bordetella avium lipopolysaccharides have been shown to be important virulence factors for this bird pathogen. B. Read More

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http://dx.doi.org/10.1016/j.biochi.2018.12.011DOI Listing
December 2018
5 Reads

l-arginine inhibited apoptosis of fish leukocytes via regulation of NF-κB-mediated inflammation, NO synthesis, and anti-oxidant capacity.

Biochimie 2019 Mar 19;158:62-72. Epub 2018 Dec 19.

Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei, 430070, China. Electronic address:

The increased apoptosis plays an important role in bacterial invasion. In addition, LPS can induce inflammation and apoptosis of leukocytes via the production of reactive oxygen and nitrogen species. In the present study, we investigated the potential protective role of l-arginine (L-Arg) against the apoptosis of fish leukocytes in vitro. Read More

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http://dx.doi.org/10.1016/j.biochi.2018.12.010DOI Listing
March 2019
2 Reads

Inhibitor of polyamine catabolism MDL72.527 restores the sensitivity to doxorubicin of monocytic leukemia Thp-1 cells infected with human cytomegalovirus.

Biochimie 2019 Mar 19;158:82-89. Epub 2018 Dec 19.

Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia. Electronic address:

Leukemic cells from different patients exhibit different sensitivity to anticancer drugs including doxorubicin (DOX). Resistance to chemotherapy decreases efficacy of the treatment and promotes cancer recurrence and metastases. One of the approaches to overcome drug resistance includes E2F1-mediated regulation of the р73 protein that belongs to the р53 family. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S03009084183035
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http://dx.doi.org/10.1016/j.biochi.2018.12.012DOI Listing
March 2019
9 Reads

Role of human Keap1 S53 and S293 residues in modulating the binding of Keap1 to Nrf2.

Biochimie 2019 Mar 18;158:73-81. Epub 2018 Dec 18.

Hainan Province Key Laboratory for Sustainable Utilization of Tropical Bioresources, Hainan University, Hainan, 570228, China; Laboratory of Biotechnology and Molecular Pharmacology, College of Biology, Institute of Tropical Agriculture and Forestry, Hainan University, Hainan, 570228, China. Electronic address:

Keap1 is deemed as a suppressor of Nrf2 in cytoplasm by sequestrating Nrf2 to proteolysis as an adapter of the Cul3-Rbx1 E3 ubiquitin ligase complex. In the study, it was proposed that post-translational modification might affect the interaction between Nrf2 and Keap1, and the profiles of the phosphorylation of amino acid residues of Keap1 and its effects on the binding of Keap1 to Nrf2 was investigated. A mass spectrometry analysis revealed that S53 and S293 were phosphorylated upon an oxidative stress. Read More

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http://dx.doi.org/10.1016/j.biochi.2018.12.008DOI Listing
March 2019
9 Reads

Genetic depletion of p53 attenuates cocaine-induced hepatotoxicity in mice.

Biochimie 2019 Mar 18;158:53-61. Epub 2018 Dec 18.

Neuropsychopharmacology and Toxicology Program, College of Pharmacy, Kangwon National University, Chunchon, 24341, Republic of Korea. Electronic address:

Cocaine, an addictive drug, is known to induce hepatotoxicity via oxidative damage and proapoptosis. Since p53, a tumor suppressor gene, plays a major role in inducing oxidative stress and apoptosis, we examined the role of p53 inhibition against cocaine-induced hepatotoxicity. Cocaine treatment significantly increased oxidative parameters (i. Read More

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http://dx.doi.org/10.1016/j.biochi.2018.12.009DOI Listing
March 2019
4 Reads
2.963 Impact Factor

Parathyroid hormone-stimulation of Runx2 during osteoblast differentiation via the regulation of lnc-SUPT3H-1:16 (RUNX2-AS1:32) and miR-6797-5p.

Biochimie 2019 Mar 15;158:43-52. Epub 2018 Dec 15.

Department of Biotechnology, School of Bioengineering, SRM Institute of Science and Technology, Kattankulathur, Tamil Nadu, India. Electronic address:

Parathyroid hormone (PTH) acts as a regulator of calcium homeostasis and bone remodeling. Runx2, an essential transcription factor in bone, is required for osteoblast differentiation. Noncoding RNAs such as long noncoding RNAs (lncRNAs) and microRNAs (miRNAs) play crucial roles in regulating gene expression in osteoblasts. Read More

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http://dx.doi.org/10.1016/j.biochi.2018.12.006DOI Listing
March 2019
5 Reads

Dioclea violacea lectin ameliorates inflammation in the temporomandibular joint of rats by suppressing intercellular adhesion molecule-1 expression.

Biochimie 2019 Mar 14;158:34-42. Epub 2018 Dec 14.

Faculdade São Leopoldo Mandic, Instituto de Pesquisas São Leopoldo Mandic, Área de Imunologia, Campinas, Brazil. Electronic address:

Inflammation of temporomandibular joint (TMJ) tissues are the most common cause of pain conditions associated with temporomandibular disorders (TMDs). After a tissue and/or neural damage, the inflammatory response is characterized by plasma extravasation and leukocytes infiltration in the TMJ tissues, which in turn, release inflammatory cytokines cascades responsible for inflammatory pain. Lectins are glycoproteins widely distributed in nature that may exhibit anti-inflammatory properties. Read More

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http://dx.doi.org/10.1016/j.biochi.2018.12.007DOI Listing
March 2019
3 Reads

Ribosomal protein eL42 contributes to the catalytic activity of the yeast ribosome at the elongation step of translation.

Biochimie 2019 Mar 11;158:20-33. Epub 2018 Dec 11.

Sorbonne Université, Campus Pierre et Marie Curie, Laboratoire "Enzymologie de l'ARN", SU-UR6, (Bâtiment B), Case courrier 60 - 4, Place Jussieu, F-75252, Paris Cedex 05, France.

The GGQ minidomain of the ribosomal protein eL42 was previously shown to contact the CCA-arm of P-site bound tRNA in human ribosome, indicating a possible involvement of the protein in the catalytic activity. Here, using Schizosaccharomyces pombe (S. pombe) cells, we demonstrate that the GGQ minidomain and neighboring region of eL42 is critical for the ribosomal function. Read More

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http://dx.doi.org/10.1016/j.biochi.2018.12.005DOI Listing
March 2019
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TRPC channels mediated calcium entry is required for proliferation of human airway smooth muscle cells induced by nicotine-nAChR.

Biochimie 2019 Mar 11;158:139-148. Epub 2018 Dec 11.

Respiratory Medicine, Hunan Provincial People's Hospital, No. 61 Jiefang Xi Road, Changsha, 410219, Hunan, PR China; Institute of Respiratory Medicine, Changsha Medical College, No. 1501 Leifeng Road, Changsha, 410219, Hunan, PR China. Electronic address:

The present study was designed to explore the role of transient receptor potential canonical 3 (TRPC3) in nicotine-induced chronic obstructive pulmonary disease (COPD) and its underlying mechanism. In this study, the expression and localization of α5 nicotinic acetylcholine receptor (α5-nAchR) in lung tissues were determined by western blotting and immunohistochemistry. The quantitative real-time PCR (qRT-PCR) analysis was performed to examine the mRNA expression levels of α5-nAchR and TRPC3 in human airway smooth muscle cells (HASMCs). Read More

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http://dx.doi.org/10.1016/j.biochi.2018.12.004DOI Listing
March 2019
1 Read
2.963 Impact Factor

MiR-204-5p promotes apoptosis and inhibits migration of osteosarcoma via targeting EBF2.

Biochimie 2019 Mar 6;158:224-232. Epub 2018 Dec 6.

Department of Orthopedics, The 306th Hospital of PLA, Beijing, 100101, China. Electronic address:

Osteosarcoma is one of the most malignant cancer adolescents and young adults and metastatic osteosarcoma is a huge life threat with a 5-year survival lower than 20%. However, the mechanisms through which localized osteosarcoma turned metastatic are not fully understood. Here, we studied the role of miR-204-5p in osteosarcoma and found that miR-204-5p is downregulated in both osteosarcoma patients and osteosarcoma cell lines. Read More

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http://dx.doi.org/10.1016/j.biochi.2018.12.003DOI Listing
March 2019
2 Reads

Folate and choline absorption and uptake: Their role in fetal development.

Biochimie 2019 Mar 6;158:10-19. Epub 2018 Dec 6.

Institute of Human Nutrition and Dietetics, Poznań University of Life Sciences, Poland.

Scope: In this review, we attempt to assess how choline and folate transporters affect fetal development. We focus on how the expression of these transporters in response to choline and folate intake affects transport effectiveness. We additionally describe allelic variants of the genes encoding these transporters and their phenotypic effects. Read More

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http://dx.doi.org/10.1016/j.biochi.2018.12.002DOI Listing
March 2019
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SOS genes contribute to Bac8c induced apoptosis-like death in Escherichia coli.

Biochimie 2019 Feb 5;157:195-203. Epub 2018 Dec 5.

School of Life Sciences, BK 21 Plus KNU Creative BioResearch Group, College of Natural Sciences, Kyungpook National University, Daehakro 80, Bukgu, Daegu, 41566, Republic of Korea. Electronic address:

Antimicrobial peptides are one of the promising substitutes for conventional antibiotics. To examine their potential usefulness for controlling bacterial infections, the present study aimed to determine the induction of the self-destruction of Escherichia coli mediated by bac8c, which was modified from template peptide bactenecin. The potential of bac8c (RIWVIWRR) was reflected by the change in physiological following exposure, which led to the accumulation of reactive oxygen species (ROS) with an imbalance in the antioxidant system and damage to the intracellular lipids. Read More

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http://dx.doi.org/10.1016/j.biochi.2018.12.001DOI Listing
February 2019
2 Reads

Endonuclease G modulates the alternative splicing of deoxyribonuclease 1 mRNA in human CD4 T lymphocytes and prevents the progression of apoptosis.

Biochimie 2019 Feb 3;157:158-176. Epub 2018 Dec 3.

Institute of Biomedical Chemistry, Pogodinskaya St 10/8, 119121, Moscow, Russia.

Apoptotic endonucleases act cooperatively to fragment DNA and ensure the irreversibility of apoptosis. However, very little is known regarding the potential regulatory links between endonucleases. Deoxyribonuclease 1 (DNase I) inactivation is caused by alternative splicing (AS) of DNase I pre-mRNA skipping exon 4, which occurs in response to EndoG overexpression in cells. Read More

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http://dx.doi.org/10.1016/j.biochi.2018.11.020DOI Listing
February 2019
6 Reads

Thermodynamic investigation of kissing-loop interactions.

Biochimie 2019 Feb 28;157:177-183. Epub 2018 Nov 28.

Department of Pharmaceutical Sciences, University of Nebraska Medical Center, 986025 Nebraska Medical Center, Omaha, NE, 68198-6025, USA. Electronic address:

Kissing loop interactions (KLIs) are a common motif that is critical in retroviral dimerization, viroid replication, mRNA, and riboswitches. In addition, KLIs are currently used in a variety of biotechnology applications, such as in aptamer sensors, RNA scaffolds and to stabilize vaccines for therapeutics. Here we describe the thermodynamics of a basic intramolecular DNA capable of engaging in a KLI, consisting of two hairpins connected by a flexible linker. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S03009084183033
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http://dx.doi.org/10.1016/j.biochi.2018.11.012DOI Listing
February 2019
13 Reads
2.963 Impact Factor