8,451 results match your criteria Biochimie[Journal]


Vitexin abrogates invasion and survival of hepatocellular carcinoma cells through targeting STAT3 signaling pathway.

Biochimie 2020 May 20. Epub 2020 May 20.

College of Korean Medicine, Kyung Hee University, #47, Kyungheedae-gil, Dongdaemoon-gu, Seoul, 130-701, Republic of Korea. Electronic address:

Hepatocellular carcinoma (HCC) is a major malignancy that stands second in terms of global cancer-related mortality. STAT3 has been described as a latent transcription factor that promotes tumorigenesis. This study was designed to examine the effect of vitexin on STAT3 signaling and important hallmarks of cancer. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biochi.2020.05.006DOI Listing

Preventive as well as therapeutic significances of linoleic acid in the containment of Leishmania donovani infection.

Biochimie 2020 May 18. Epub 2020 May 18.

Department of Biotechnology, Motilal Nehru National Institute of Technology (MNNIT), Allahabad, 211004, India. Electronic address:

People suffering from malnutrition show compromised levels of ω-6 fatty acid and malnutrition is frequently observed among visceral leishmaniasis (VL) patients as disease inflicts primarily the socioeconomic destitute communities. Dietary linoleic acid (LA, 18:2; ω-6 fatty acid) is the principal source of essential fatty acid and its derivatives i.e. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biochi.2020.04.024DOI Listing

The beclin 1 interactome: Modification and roles in the pathology of autophagy-related disorders.

Biochimie 2020 May 16. Epub 2020 May 16.

Department of Biotechnology and Bioinformatics, Jaypee University of Information Technology, Solan, 173 234, Himachal Pradesh, India. Electronic address:

Beclin 1 a yeast Atg6/VPS30 orthologue has a significant role in autophagy process (Macroautophagy) and protein sorting. The function of beclin 1 depends on the interaction with several autophagy-related genes (Atgs) and other proteins during the autophagy process. The role mediated by beclin 1 is controlled by various conditions and factors. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biochi.2020.04.025DOI Listing

Crystal structures and RNA-binding properties of Lsm proteins from archaea Sulfolobus acidocaldarius and Methanococcus vannielii: Similarity and difference of the U-binding mode.

Biochimie 2020 May 15. Epub 2020 May 15.

Institute of Protein Research Russian Academy of Sciences, Institutskaya 4, Moscow Region, Pushchino, 142290, Russia. Electronic address:

Sm and Sm-like (Lsm) proteins are considered as an evolutionary conserved family involved in RNA metabolism in organisms from bacteria and archaea to human. Currently, the function of Sm-like archaeal proteins (SmAP) is not well understood. Here, we report the crystal structures of SmAP proteins from Sulfolobus acidocaldarius and Methanococcus vannielii and a comparative analysis of their RNA-binding sites. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biochi.2020.05.001DOI Listing

GRP78 and next generation cancer hallmarks: An underexplored molecular target in cancer chemoprevention research.

Biochimie 2020 May 15. Epub 2020 May 15.

Cancer Biology Laboratory, Anusandhan Kendra II, School of Chemical and Biotechnology, SASTRA Deemed University, Thirumalaisamudram, Thanjavur, 613401, Tamil Nadu, India. Electronic address:

Glucose regulated protein 78 (GRP 78), a master regulator of endoplasmic reticulum stress has been reported to be up regulated in various cancers and remains a crucial link between tumor glycolysis and tumor microenvironment. Overexpressed GRP78 has also shown to induce immune suppressive molecules and thereby tumor immune evasion. On the other hand emerging reports indicates that the next generation hallmarks viz. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biochi.2020.05.005DOI Listing

Tough synthetic spider-silk fibers obtained by titanium dioxide incorporation and formaldehyde cross-linking in a simple wet-spinning process.

Biochimie 2020 May 14. Epub 2020 May 14.

Institute of Biological Sciences and Biotechnology, Donghua University, Shanghai 201620, China. Electronic address:

Due to its unique mechanical properties, spider silk shows great promise as a strong super-thin fiber in many fields. Although progress has been made in the field of synthesizing spider-silk fiber from recombinant spidroin (spider silk protein) in the last few decades, methods to obtain synthetic spider-silk fibers as tough as natural silk from small-sized recombinant protein with a simple spinning process have eluded scientists. In this paper, a recombinant spidroin (MW: 93. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biochi.2020.05.003DOI Listing

A glance into the evolution of template-free protein structure prediction methodologies.

Biochimie 2020 May 14. Epub 2020 May 14.

Université de Nantes, CNRS, UFIP, UMR6286, F-44000, Nantes, France. Electronic address:

Prediction of protein structures using computational approaches has been explored for over two decades, paving a way for more focused research and development of algorithms in comparative modelling, ab intio modelling and structure refinement protocols. A tremendous success has been witnessed in template-based modelling protocols, whereas strategies that involve template-free modelling still lag behind, specifically for larger proteins (>150 a.a. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biochi.2020.04.026DOI Listing

Acute exposure to bis(2-ethylhexyl)phthalate disrupts calcium homeostasis, energy metabolism and induces oxidative stress in the testis of Danio rerio.

Biochimie 2020 May 14. Epub 2020 May 14.

Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, CEP: 88040-900, Florianópolis, Santa Catarina, Brazil. Electronic address:

Bis(2-ethylhexyl)phthalate (BEHP) negatively affects testicular functions in different animal species, disturbing reproductive physiology and male fertility. The present study investigated the in vitro acute effect of BEHP on the mechanism of action of ionic calcium (Ca) homeostasis and energy metabolism. In addition, the effect of BEHP on oxidative stress was studied in vitro and in vivo in the testis of Danio rerio (D. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biochi.2020.05.002DOI Listing

Protein promiscuity in drug discovery, drug-repurposing and antibiotic resistance.

Biochimie 2020 May 13. Epub 2020 May 13.

JH-Institute of Molecular Medicine, Jamia Hamdard, New Delhi, 110062, India; Dr Reddy's Institute of Life Sciences, University of Hyderabad Campus, Professor CR Rao Road, Hyderabad, 500046, India. Electronic address:

Proteins are supposed to bind to their substrates/ligands in a specific manner via their pre-formed binding sites, according to classical biochemistry. In recent years, several types of deviations from this norm have been observed and called promiscuous behavior. Enzymatic promiscuities allow several biochemical functions to be carried out by the same enzyme. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biochi.2020.05.004DOI Listing
May 2020
2.963 Impact Factor

Human health benefits of very-long-chain polyunsaturated fatty acids from microalgae.

Biochimie 2020 May 7. Epub 2020 May 7.

MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing, MI, 48824, USA; Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI, 48824, USA; Department of Plant Biology, Michigan State University, East Lansing, MI, 48824, USA.

Microalgae are single-cell, photosynthetic organisms whose biodiversity places them at the forefront of biological producers of high-value molecules including lipids and pigments. Some of these organisms particular are capable of synthesizing n-3 very long chain polyunsaturated fatty acids (VLC-PUFAs), known to have beneficial effects on human health. Indeed, VLC-PUFAs are the precursors of many signaling molecules in humans involved in the complexities of inflammatory processes. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biochi.2020.04.022DOI Listing

Folinic acid improves the score of Autism in the EFFET placebo-controlled randomized trial.

Biochimie 2020 May 7. Epub 2020 May 7.

Inserm UMRS 1256 N-GERE (Nutrition-Genetics-Environmental Risks), Regional University Hospital of Nancy and University of Lorraine, BP 184, 54511, Nancy (Vandoeuvre), France. Electronic address:

Autism spectrum disorders (ASD) are influenced by interacting maternal and environmental risk factors. High-dose folinic acid has shown improvement in verbal communication in ASD children. The EFFET randomized placebo-controlled trial (NCT02551380) aimed to evaluate the efficacy of folinic acid (FOLINORAL®) at a lower dose of 5 mg twice daily. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biochi.2020.04.019DOI Listing

Alternagin-C (ALT-C), a disintegrin-like protein, attenuates alpha2beta1 integrin and VEGF receptor 2 signaling resulting in angiogenesis inhibition.

Biochimie 2020 Apr 30;174:144-158. Epub 2020 Apr 30.

Biochemistry and Molecular Biology Laboratory, Department of Physiological Sciences, Federal University of São Carlos, Rod. Washington Luís, km 235 - SP-310 - São Carlos, São Paulo, CEP 13565-905, Brazil.

Angiogenesis, a crucial process in tumor progression, is mainly regulated by vascular endothelial growth factor (VEGF) and its receptor, VEGFR2. Studies have shown the interaction between αβ integrin, a collagen receptor, and VEGFR2 in VEGF-driven angiogenesis in vitro and in vivo. Alternagin-C (ALT-C), an ECD-disintegrin-like protein from Bothrops alternatus snake venom, has high affinity for αβ integrin and shows antiangiogenic activity in concentrations higher than 100 nM. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biochi.2020.04.023DOI Listing

StpA represses CRISPR-Cas immunity in H-NS deficient Escherichia coli.

Biochimie 2020 Apr 28;174:136-143. Epub 2020 Apr 28.

Department of Biology, Faculty of Science, University of Zagreb, 10000, Zagreb, Croatia. Electronic address:

Functional CRISPR-Cas systems provide many bacteria and most archaea with adaptive immunity against invading DNA elements. CRISPR arrays store DNA fragments of previous infections while products of cas genes provide immunity by integrating new DNA fragments and using this information to recognize and destroy invading DNA. Escherichia coli contains the CRISPR-Cas type I-E system in which foreign DNA targets are recognized by Cascade, a crRNA-guided complex comprising five proteins (CasA, CasB, CasC, CasD, CasE), and degraded by Cas3. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biochi.2020.04.020DOI Listing

Physico-chemical properties of two point mutants of small heat shock protein HspB6 (Hsp20) with abrogated cardioprotection.

Biochimie 2020 Apr 27;174:126-135. Epub 2020 Apr 27.

Department of Biochemistry, School of Biology, Moscow State University, Moscow, 119991, Russian Federation. Electronic address:

Physico-chemical properties of HspB6 S10F and P20L mutants with abrogated cardioprotective activity and associated with different forms of cardiomyopathy were analyzed. Under normal conditions both the wild-type HspB6 and its mutants formed small size oligomers (dimers) with apparent molecular weight of 50-60 kDa. Under crowding conditions (0. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biochi.2020.04.021DOI Listing

SPCA1 governs the stability of TMEM165 in Hailey-Hailey disease.

Biochimie 2020 Apr 23;174:159-170. Epub 2020 Apr 23.

Univ. Lille, CNRS, UMR 8576 - UGSF - Unité de Glycobiologie Structurale et Fonctionnelle, F-59000, Lille, France. Electronic address:

TMEM165 is a Golgi protein whose deficiency causes a Congenital Disorder of Glycosylation (CDG). We have demonstrated that Mn supplementation could suppress the glycosylation defects observed in TMEM165-deficient cells and that TMEM165 was a Mn-sensitive protein. In the Golgi, the other transmembrane protein capable to regulate Mn/Ca homeostasis is SPCA1, encoded by the ATP2C1 gene. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biochi.2020.04.017DOI Listing

Murine cellular model of mucopolysaccharidosis, type IIIB (MPS IIIB) - A preliminary study with particular emphasis on the non-oxidative l-cysteine metabolism.

Biochimie 2020 Apr 23;174:84-94. Epub 2020 Apr 23.

Jagiellonian University, Medical College, Faculty of Medicine, Chair of Medical Biochemistry, Kopernika 7 St., 31-034, Krakow, Poland.

The lack of the N-alpha-glucosaminidase (Naglu) is responsible for the incidence of a rare disease - mucopolysaccharidosis, type IIIB (MPS IIIB). To date, studies have been conducted based on cells derived from patients suffering from MPS or using in vivo MPS mouse models. These limitations have allowed for defining our research goal - to create and characterize the first in vitro murine cellular MPS IIIB model. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biochi.2020.04.015DOI Listing

Conformational selection in the flaviviral NS2B-NS3 protease.

Biochimie 2020 Apr 23;174:117-125. Epub 2020 Apr 23.

Medicinal Chemistry, Institute of Pharmacy and Molecular Biotechnology IPMB, Heidelberg University, Im Neuenheimer Feld 364, 69120, Heidelberg, Germany. Electronic address:

The first x-ray structures of flaviviral proteases defined two conformational states, open and closed, depending on the relative position of NS2B with respect to NS3, a feature that affects the shape of the binding site. The degree of flexibility in the active site was limited to changes in the fold of NS2B rather than NS3 and an induced-fit mechanism was regarded as the main factor for ligand binding. A minor degree of conformational plasticity in NS3 is observed in the two protein chains in the asymmetric unit for the structure of Zika protease with a dipeptide boronate, synthesized in our group. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biochi.2020.04.014DOI Listing

Riboflavin supplementation alters global and gene-specific DNA methylation in adults with the MTHFR 677 TT genotype.

Biochimie 2020 Apr 22. Epub 2020 Apr 22.

Genomic Medicine Research Group, Ulster University, Coleraine, Northern Ireland, United Kingdom. Electronic address:

DNA methylation is important in regulating gene expression and genomic stability while aberrant DNA methylation is associated with disease. Riboflavin (FAD) is a cofactor for methylenetetrahydrofolate reductase (MTHFR), a critical enzyme in folate recycling, which generates methyl groups for homocysteine remethylation to methionine, the pre-cursor to the universal methyl donor S-adenosylmethionine (SAM). A polymorphism (C677T) in MTHFR results in decreased MTHFR activity and increased homocysteine concentration. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biochi.2020.04.007DOI Listing

B vitamins and one carbon metabolism micronutrients in health and disease.

Biochimie 2020 Apr 22. Epub 2020 Apr 22.

Inserm and Medical Faculty of University of Lorraine, Nancy-Vandoeuvre, France. Electronic address:

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biochi.2020.04.018DOI Listing

Knockdown of the α5 laminin chain affects differentiation of colorectal cancer cells and their sensitivity to chemotherapy.

Biochimie 2020 Apr 22;174:107-116. Epub 2020 Apr 22.

Faculty of Biology and Biotechnology, National Research University Higher School of Economics, Myasnitskaya str. 13/4, 117997, Moscow, Russia; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Miklukho-Maklaya str. 16/10, 117997, Moscow, Russia; Scientific Research Center Bioclinicum, Ugreshskaya str. 2/85, 115088, Moscow, Russia. Electronic address:

The interaction of tumor cells with the extracellular matrix (ECM) may affect the rate of cancer progression and metastasis. One of the major components of ECM are laminins, the heterotrimeric glycoproteins consisting of α-, β-, and γ-chains (αβγ). Laminins interact with their cell surface receptors and, thus, regulate multiple cellular processes. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biochi.2020.04.016DOI Listing

Impact of the MTHFR C677T polymorphism on one-carbon metabolites: Evidence from a randomised trial of riboflavin supplementation.

Biochimie 2020 Apr 21. Epub 2020 Apr 21.

Nutrition Innovation Centre for Food and Health (NICHE), Ulster University, Cromore Rd, Coleraine, BT52 1SA, Northern Ireland, UK. Electronic address:

Homozygosity for the C677T polymorphism in MTHFR (TT genotype) is associated with a 24-87% increased risk of hypertension. Blood pressure (BP) lowering was previously reported in adults with the TT genotype, in response to supplementation with the MTHFR cofactor, riboflavin. Whether the BP phenotype associated with the polymorphism is related to perturbed one-carbon metabolism is unknown. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biochi.2020.04.004DOI Listing

Tetracycline derivatives resist the assembly behavior of human islet amyloid polypeptide.

Biochimie 2020 Apr 21;174:95-106. Epub 2020 Apr 21.

Department of Chemistry, Renmin University of China, Beijing, 100872, China. Electronic address:

The misfolding of amyloid proteins is closely correlated with the pathogenesis of protein conformation-related diseases, such as Alzheimer's disease (AD), prion disease, and type 2 diabetes mellitus (T2DM). The deposition of human islet amyloid polypeptide (hIAPP) and amyloid-β (Aβ) protein is entangled in AD and diabetes mellitus. The development of potential inhibitors is a feasible therapeutic strategy to treat these diseases by resisting peptide aggregation. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biochi.2020.04.012DOI Listing

Differential levels of Alpha-1-inhibitor III, Immunoglobulin heavy chain variable region, and Hypertrophied skeletal muscle protein GTF3 in rat mammary tumorigenesis.

Biochimie 2020 Apr 20;174:57-68. Epub 2020 Apr 20.

Department of Biotechnology, School of Chemical and Life Sciences, JamiaHamdard, New Delhi, 110062, India. Electronic address:

Early detection of breast cancer can be best facilitated by the development of precancerous markers. Serum proteins being the sensitive signatures, can be the ideal choice. We previously demonstrated the reduced levels of two serum proteins at a very early stage of tumorigenesis in a breast cancer model, developed in Wistar rats by 7,12-dimethylbenz[a]anthracene (DMBA) administration. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biochi.2020.04.013DOI Listing
April 2020
2.963 Impact Factor

Fusion-PCR generates attL recombination site adaptors and allows Rapid One-Step Gateway (ROG) cloning.

Biochimie 2020 Apr 20;174:69-73. Epub 2020 Apr 20.

Key Laboratory of Plant Genetics and Molecular Breeding, Zhoukou Normal University, Zhoukou, 466001, China; Henan Key Laboratory of Crop Molecular Breeding & Bioreactor, Zhoukou, 466001, China; Henan Engineering Research Center of Crop Genome Editing, Henan Institute of Science and Technology, Xinxiang, 453003, China. Electronic address:

Gateway recombination-based cloning, which eliminates the use of restriction endonucleases and ligase, has been widely used for the construction of high-throughput (HTP) vectors. However, this approach is very expensive and its two-stage reaction process is laborious and time consuming. Therefore, we developed a Gateway cloning method that uses fusion-PCR to generate attL recombination site adaptors, and the PCR products, which can be directly cloned into destination vectors, giving rise to Rapid One-Step Gateway (ROG) Cloning. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biochi.2020.04.002DOI Listing
April 2020
2.963 Impact Factor

Inhibition of nuclease activity by a splice-switching oligonucleotide targeting deoxyribonuclease 1 mRNA prevents apoptosis progression and prolong viability of normal human CD4 T-lymphocytes.

Biochimie 2020 Apr 18;174:34-43. Epub 2020 Apr 18.

Institute of Biomedical Chemistry, Pogodinskaya st. 10/8, 119121, Moscow, Russia.

The nuclease activity of deoxyribonuclease 1 (DNase I) is regulated by alternative splicing (AS) of its mRNA. The aim of this study was to define the ability of a splice-switching oligonucleotide (SSO) that base-paired with DNase I pre-mRNA to induce AS and inhibit nuclease activity in human T, B and NK lymphocytes. The SSO for DNase I could significantly downregulate the expression of full-length active DNase I and upregulate a truncated splice variant with a deleted exon 4. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biochi.2020.04.009DOI Listing

ARE-binding protein ZFP36L1 interacts with CNOT1 to directly repress translation via a deadenylation-independent mechanism.

Biochimie 2020 Apr 18;174:49-56. Epub 2020 Apr 18.

Kindai University, Higashi, Osaka, Japan. Electronic address:

Eukaryotic gene expression can be spatiotemporally tuned at the post-transcriptional level by cis-regulatory elements in mRNA sequences. An important example is the AU-rich element (ARE), which induces mRNA destabilization in a variety of biological contexts in mammals and can also mediate translational control. Regulation is mediated by trans-acting factors that recognize the ARE, such as Tristetraprolin (TTP) and BRF1/ZFP36L1. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biochi.2020.04.010DOI Listing

Aberrant interactions between amyloid-beta and alpha5 laminins as possible driver of neuronal disfunction in Alzheimer's disease.

Biochimie 2020 Apr 18;174:44-48. Epub 2020 Apr 18.

Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia.

It has been widely accepted that laminins are involved in pathogenesis of Alzheimer's disease (AD). Amyloid plaques in AD patients are associated with immunostaining using antibodies raised against laminin-111, and laminin-111 has been shown to prevent aggregation of amyloid peptides. Although numerous articles describe small peptides from laminin-111 that are capable to disaggregate amyloid buildups and reduce neurotoxicity in in vitro and in vivo models, there is no approved laminin-111-based therapeutic approaches for treatment of AD. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biochi.2020.04.011DOI Listing

Renin-angiotensin system at the heart of COVID-19 pandemic.

Biochimie 2020 Apr 16;174:30-33. Epub 2020 Apr 16.

Digestive Disease Department, Archet 2 Hospital, Nice University Hospital, University of Nice Côte d'Azur, Nice, France; Inserm, U1065, Team 8 "Hepatic Complications of Obesity", University Nice Côte d'Azur, France.

Significant aspects of COVID-19 pandemic remain obscure. Angiotensin converting enzyme 2 (ACE2), a component of the renin-angiotensin system, whose expression dominates on lung alveolar epithelial cells, is the human cell receptor of SARS-CoV-2, the causative agent of COVID-19. We strongly encourage the concept that thorough considerations of receptor-ligand interactions should be kept at the heart of scientific debate on infection. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biochi.2020.04.008DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7161528PMC

EDA2R mediates podocyte injury in high glucose milieu.

Biochimie 2020 Apr 15;174:74-83. Epub 2020 Apr 15.

Feinstein Institute for Medical Research and Donald and Barbara Zucker School of Medicine at Hofstra/Northwell, Manhasset, NY, United States. Electronic address:

EDA2R is a member of the large family of tumor necrosis factor receptor (TNFR). Previous studies suggested that EDA2R expression might be increased in the kidneys of diabetic mice. However, its mRNA and protein expression in kidneys were not analyzed; moreover, its role in the development of diabetic kidney disease was not explored. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biochi.2020.04.003DOI Listing

Effects of folic acid withdrawal on transcriptomic profiles in murine triple-negative breast cancer cell lines.

Biochimie 2020 Apr 15. Epub 2020 Apr 15.

Department of Nutrition, University of North Carolina at Chapel Hill, Chapel Hill, USA; Nutrition Research Institute, University of North Carolina at Chapel Hill, Kannapolis, USA. Electronic address:

We have previously shown that withdrawal of folic acid led to metabolic reprogramming and a less aggressive phenotype in a mouse cell model of triple-negative breast cancer (TNBC). Herein, we evaluate the effects of folic acid withdrawal on transcriptomic profiles in these cells. Murine cell lines were originally derived from a pool of spontaneous mammary tumors grown in MMTV-Wnt1 transgenic mice. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biochi.2020.04.005DOI Listing

Toxinological characterization of venom from Leptodeira annulata (Banded cat-eyed snake; Dipsadidae, Imantodini).

Biochimie 2020 Apr 14;174:171-188. Epub 2020 Apr 14.

Departamento de Farmacologia, Faculdade de Ciências Médicas, Universidade Estadual de Campinas (UNICAMP), Rua Tessália Vieira de Camargo, 126, Cidade Universitária Zeferino Vaz, 13083-887, Campinas, SP, Brazil. Electronic address:

We investigated the histology of Duvernoy's venom gland and the biochemical and biological activities of Leptodeira annulata snake venom. The venom gland had a lobular organization, with secretory tubules formed by serous epithelial cells surrounding each lobular duct. The latter drained into a common lobular duct and subsequently into a central cistern. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biochi.2020.04.006DOI Listing

Pharmacologic inhibition of N-linked glycan trimming with kifunensine disrupts GLUT1 trafficking and glucose uptake.

Biochimie 2020 Apr 13;174:18-29. Epub 2020 Apr 13.

Calvin College, Department of Chemistry & Biochemistry, 1726 Knollcrest Circle SE, Grand Rapids, MI 49546, USA. Electronic address:

The facilitative glucose transport GLUT1 (SLC2A1) is a constitutively expressed membrane protein involved in basal uptake of blood glucose. GLUT1 modification by N-linked glycosylation at a single asparagine residue (N45) appears to play multiple roles in the trafficking, stability and transport activity of this protein. Here we examine the role of complex N-glycosylation on GLUT1 function in renal epithelial cells by arresting this modification at the high-mannose stage with the mannosidase I inhibitor kifunensine. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biochi.2020.04.001DOI Listing

Critical review of nutrition, blood pressure and risk of hypertension through the lifecycle: do B vitamins play a role?

Biochimie 2020 Apr 11. Epub 2020 Apr 11.

Nutrition Innovation Centre for Food and Health (NICHE), School of Biomedical Sciences, Ulster University, Coleraine, Northern Ireland, BT52 1SA, United Kingdom. Electronic address:

Hypertension is the leading cause of preventable mortality worldwide, contributing to over 9 million deaths per annum, predominantly owing to cardiovascular disease. The association of obesity, physical inactivity and alcohol with elevated blood pressure (BP) is firmly established. Weight loss or other dietary strategies, such as the Dietary Approaches to Stop Hypertension (DASH) diet, have been shown to be effective in lowering BP. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biochi.2020.03.016DOI Listing

Genetic, epigenetic and genomic mechanisms of methionine dependency of cancer and tumor-initiating cells: What could we learn from folate and methionine cycles.

Biochimie 2020 Apr 11. Epub 2020 Apr 11.

INSERM UMR_S 1256 NGERE - Nutrition, Genetics, and Environmental Risk Exposure and National Center of Inborn Errors of Metabolism, University of Lorraine, Nancy (Vandoeuvre-lès-Nancy), F-54000, France.

Methionine-dependency is a common feature of cancer cells, which cannot proliferate without constant inputs of exogenous methionine even in the presence of its precursor, homocysteine. The endogenous synthesis of methionine is catalyzed by methionine synthase, which transfers the methyl group of 5-methyltetrahydrofolate (5-methylTHF) to homocysteine in the presence of vitamin B12 (cobalamin, cbl). Diverse mechanisms can produce it, including somatic mutations, aberrant DNA methylation (epimutations) and altered expression of genes. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biochi.2020.03.015DOI Listing

Regulatory T cells in patients with early untreated rheumatoid arthritis: Phenotypic changes in the course of methotrexate treatment.

Biochimie 2020 Apr 7;174:9-17. Epub 2020 Apr 7.

Department of Vascular Problems in Rheumatic Diseases, V.A. Nasonova Research Institute of Rheumatology, 115522, Moscow, Kashirskoe Shosse 34A, Russian Federation. Electronic address:

Rheumatoid arthritis (RA) is frequent systemic autoimmune disease characterized by excessive activation of collagen-specific T helper cells, and elevated level of autoantibodies in the serum. Development of RA is associated with defect in compartment of regulatory CD4+Foxp3+ T cells (Treg), but data concerning suppressive potential of Treg population in RA patients are contradictory and depend on the stage of disease. In this study we aimed to characterize abundance and phenotypic markers of CD4+Foxp3+ Treg in peripheral blood of healthy donors compared to untreated early RA patients to find potential correlations with the disease activity, antibody level, and absolute numbers and proportion of different subpopulations of T cells. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biochi.2020.03.014DOI Listing

Unique functional properties of slow skeletal muscle tropomyosin.

Biochimie 2020 Mar 26;174:1-8. Epub 2020 Mar 26.

A. N. Bach Institute of Biochemistry, Research Center of Biotechnology, Russian Academy of Sciences, Moscow, 119071, Russia; A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow, 119234, Russia. Electronic address:

Tropomyosin (Tpm) is an α-helical coiled-coil actin-binding protein playing an essential role in the regulation of muscle contraction. The α- (Tpm 1.1) and γ- (Tpm 3. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biochi.2020.03.013DOI Listing

Protein-protein interactions of ER-resident selenoproteins with their physiological partners.

Biochimie 2020 Apr - May;171-172:197-204. Epub 2020 Mar 15.

Federal State Institution of Science Institute of Cell Biophysics, Russian Academy of Sciences, 142290, Pushchino, Moscow Region, Institutskaya St. 3, Russia. Electronic address:

ER is a highly specialized complex of branched microtubules enclosed in a membrane and communicating with each other, its functions in the cell are important and very diverse: lipid and phospholipid synthesis, calcium storage, hormone synthesis, protein synthesis and maturation, membrane production, toxin neutralization, etc. The high concentration of calcium ions and the oxidizing properties of the contents of the ER cavities contribute to the proper synthesis and folding of proteins designed for secretion or exposure on the surface of the cell membrane. However, disturbance of redox regulation can lead to the accumulation of improperly folded proteins in the ER, disruption of calcium regulation, which can cause ER-stress. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biochi.2020.03.012DOI Listing

Development and validation of a rapid, specific and sensitive LC-MS/MS bioanalytical method for eicosanoid quantification - assessment of arachidonic acid metabolic pathway activity in hypertensive rats.

Biochimie 2020 Apr - May;171-172:223-232. Epub 2020 Mar 13.

Jagiellonian University, Jagiellonian Centre for Experimental Therapeutics (JCET), Bobrzynskiego 14, 30-348, Krakow, Poland; Jagiellonian University Medical College, Chair and Department of Toxicology, Medyczna 9, 30-688, Krakow, Poland. Electronic address:

Lipid mediators such as eicosanoids maintain various physiological processes, and their alterations are involved in the development of numerous cardiovascular diseases. Therefore, the reliable assessment of their profile could be helpful in diagnosis as well as in eicosanoid biomarker-based treatment. Hence, the presented study aimed to develop and validate a new rapid, specific and sensitive LC-MS/MS method for quantification of arachidonic acid-derived eicosanoids in plasma, including lipid mediators generated via COX-, LOX- and CYP450-dependent pathways. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biochi.2020.03.010DOI Listing

Structural analysis of the CARB β-lactamase from Vibrio parahaemolyticus facilitates application of the β-lactam/β-lactamase inhibitor therapy.

Biochimie 2020 Apr - May;171-172:213-222. Epub 2020 Mar 13.

Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China; Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao, China. Electronic address:

β-Lactams are the most widely used antibiotics in treating bacterial infections. However, they are rarely applied in infections caused by Vibrio parahaemolyticus, as the bacterium is intrinsically resistant to penicillins by expressing β-lactamase. Here we report structural characterization of the CARB β-lactamase from V. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biochi.2020.03.011DOI Listing

Upregulation of enzymatic antioxidants in CD4 T cells of autistic children.

Biochimie 2020 Apr - May;171-172:205-212. Epub 2020 Mar 12.

Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.

Autism spectrum disorder (ASD) is a neurodevelopmental disorder which begins in early childhood and presents itself with characteristic symptoms such as repetitive behavioral patterns and problems in speech/social interactions. Adaptive immune system is thought to be involved in the etiology of ASD. T cells orchestrate amplification of inflammation through release of inflammatory mediators; however, antioxidant defenses have not been evaluated in CD4 T cells of ASD subjects. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biochi.2020.03.009DOI Listing

In vivo evidence that bezafibrate prevents oxidative stress and mitochondrial dysfunction caused by 3-methylglutaric acid in rat liver.

Biochimie 2020 Apr - May;171-172:187-196. Epub 2020 Mar 10.

Programa de Pós-Graduação Em Ciências Biológicas: Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal Do Rio Grande Do Sul, Rua Ramiro Barcelos, 2600-Anexo, CEP 90035-003, Porto Alegre, RS, Brazil; Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal Do Rio Grande Do Sul, Rua Ramiro Barcelos, 2600-Anexo, CEP 90035-003, Porto Alegre, RS, Brazil; Programa de Pós-Graduação Em Fisiologia, Instituto de Ciências Básicas da Saúde, Universidade Federal Do Rio Grande Do Sul, Rua Sarmento Leite, 500, CEP 90035-190, Porto Alegre, RS, Brazil. Electronic address:

High urinary excretion and tissue accumulation of 3-methylglutaric acid (MGA) are observed in patients affected by 3-hydroxy-3-methylglutaric (HMGA) and 3-methylglutaconic (MGTA) acidurias. The pathomechanisms underlying the hepatic dysfunction commonly observed in these disorders are not fully elucidated so that we investigated here the effects of intraperitoneal administration of MGA on redox homeostasis, mitochondrial bioenergetics, biogenesis and dynamics in rat liver. The effects of a pre-treatment with the protective compound bezafibrate (BEZ) were also determined. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biochi.2020.03.007DOI Listing

Truncation of Huia versabilis Bowman-Birk inhibitor increases its selectivity, matriptase-1 inhibitory activity and proteolytic stability.

Biochimie 2020 Apr - May;171-172:178-186. Epub 2020 Mar 10.

Faculty of Chemistry, Department of Molecular Biochemistry, University of Gdańsk, Wita Stwosza 63, 80-308, Gdansk, Poland.

A gradual truncation of the primary structure of frog skin-derived Huia versabilis Bowman-Birk peptidic inhibitor (HV-BBI) resulted in 18-times stronger inhibitor of matriptase-1 (peptide 6, K = 8 nm) in comparison to the full-length HV-BBI (K = 155 nm). Analogous increase in the inhibitory activity in correlation with the peptide length reduction was not observed in case of other serine proteases, bovine trypsin (K = 151 nm for peptide 6 and K = 120 nm for HV-BBI) and plasmin (K = 120 nm for peptide 6 and 82 nm for HV-BBI). Weaker binding affinity to these enzymes emphasized an inhibitory specificity of peptide 6. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biochi.2020.03.006DOI Listing

Body mass index determines the response of plasma sulfur amino acids to methionine loading.

Biochimie 2020 Mar 5. Epub 2020 Mar 5.

Department of Pharmacology, University of Oxford, Oxford, UK; Department of Nutrition, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway.

Evidence from human, animal and cellular studies suggests that high plasma total cysteine (tCys) is causally linked to human obesity, but determinants of population tCys variability are unknown. We hypothesized that tCys elevation in obesity may be mediated by an altered tCys response to intake of its precursor, methionine. We investigated whether BMI influences the change in plasma tCys, total homocysteine (tHcy) and total cysteinylglycine (tCysGly) 6h following a 100 mg/kg oral methionine load in 800 healthy subjects and 750 cardiovascular disease (CVD) cases. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biochi.2020.03.001DOI Listing

Interaction of 5-substituted pyrimidine nucleoside analogues and M.Tuberculosis: A view through an electron microscope.

Biochimie 2020 Apr - May;171-172:170-177. Epub 2020 Mar 6.

Engelhardt Institute of Molecular Biology of the Russian Academy of Sciences, 32 Vavilov St., Moscow, 119991, Russia. Electronic address:

The data of transmission electron microscopy (TEM) on morphology of M. tuberculosis H37Rv bacterial cells treated with four analogues of pyrimidine nucleosides with different substituents at 5 position of base are presented. We showed that the growth of M. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biochi.2020.03.004DOI Listing

Identification and characterization of dihydropyrimidinase inhibited by plumbagin isolated from Nepenthes miranda extract.

Biochimie 2020 Apr - May;171-172:124-135. Epub 2020 Mar 5.

School of Biomedical Sciences, Chung Shan Medical University, No.110, Sec.1, Chien-Kuo N. Rd., Taichung City, Taiwan; Department of Medical Research, Chung Shan Medical University Hospital, No.110, Sec.1, Chien-Kuo N. Rd., Taichung City, Taiwan. Electronic address:

Dihydropyrimidinase is a member of the cyclic amidohydrolase family, which also includes allantoinase, dihydroorotase, hydantoinase, and imidase. This enzyme is important in pyrimidine metabolism, and blocking its activity would be detrimental to cell survival. This study investigated the dihydropyrimidinase inhibition by plumbagin isolated from the extract of carnivorous plant Nepenthes miranda (Nm). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biochi.2020.03.005DOI Listing

In vitro and computational studies of natural products related to perezone as anti-neoplastic agents.

Biochimie 2020 Apr - May;171-172:158-169. Epub 2020 Mar 5.

Química inorgánica-orgánica del Departamento de Ciencias Químicas, Facultad de Estudios Superiores Cuautitlán Campo 1, Universidad Nacional Autónoma de México, Estado de México, Mexico. Electronic address:

Many natural phyto-products as perezone (Per) exhibit anti-cancer activities. Using experimental and computational studies, it was described that Poly ADP-ribose polymerase 1(PARP-1) inhibition and the induction of oxidative stress state explain the pro-apoptotic activity of Per. The aim of this study was to evaluate two phyto-products related to Per as anti-cancer agents: hydroxyperezone (OHPer) and its monoangelate (OHPer-MAng). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biochi.2020.03.003DOI Listing

MksB, an alternate condensin from Mycobacterium smegmatis is involved in DNA binding and condensation.

Biochimie 2020 Apr - May;171-172:136-146. Epub 2020 Mar 5.

Department of Molecular Nutrition, CSIR-Central Food Technological Research Institute (CFTRI), Mysuru, Karnataka, 570020, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002, India. Electronic address:

The structural maintenance of chromosomes (SMC) proteins play a vital role in genome stability and chromosome organization in all domains of life. Previous reports show that smc deletion causes decondensation of chromosome and an increased frequency of anucleated cells in bacteria. However, smc deletion in both Mycobacterium smegmatis and Mycobacterium tuberculosis did not affect chromosome condensation or the frequency of anucleated cells. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biochi.2020.03.002DOI Listing
March 2020
2.963 Impact Factor

Exosomal Nrf2: From anti-oxidant and anti-inflammation response to wound healing and tissue regeneration in aged-related diseases.

Biochimie 2020 Apr - May;171-172:103-109. Epub 2020 Feb 26.

Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Department of Medical Nanotechnology, School of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.

Accumulation of oxidative stress in cells is an essential feature of cellular senescence and aging. This phenomenon is involved in different age-related diseases through dysregulation of homeostasis and impairing repair and regeneration (wound healing) capacity, which can suppress antioxidant responses such as the activity of antioxidant enzymes and damaged protein clearance system. Nuclear factor erythroid 2-related factor 2 (Nrf2) is a transcription factor which regulates basal and inducible expression pattern of specific genes (antioxidants and detoxifications) through antioxidant element response (ARE) sites in the stress condition, specifically in chronic and age-related stresses. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biochi.2020.02.011DOI Listing
February 2020

Molecular characterization of Ditylenchus destructor voltage-gated calcium channel α1 subunits and analysis of the effect of their knockdown on nematode activity.

Biochimie 2020 Apr - May;171-172:91-102. Epub 2020 Feb 25.

College of Plant Protection, Hunan Agricultural University, Changsha, Hunan, 410128, China; Hunan Provincial Engineering & Technology Research Center for Bio-pesticide and Formulation Processing, Changsha, Hunan, 410128, China. Electronic address:

Voltage-gated calcium channels (VGCCs) mediate the entry of Ca ions into cells in response to membrane depolarization and play fundamental roles in the nervous system, and the α1 subunits are the main subunits of Ca channels. Caenorhabditis elegans possesses genes encoding α1 subunits; however, very few of these genes have been cloned in plant-parasitic nematodes (PPNs). Ditylenchus destructor is a PPN that has been proposed as a new model for studying the biology and control of PPNs. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biochi.2020.02.010DOI Listing
February 2020

Deiminated proteins and extracellular vesicles as novel biomarkers in pinnipeds: Grey seal (Halichoerus gryptus) and harbour seal (Phoca vitulina).

Biochimie 2020 Apr - May;171-172:79-90. Epub 2020 Feb 24.

Tissue Architecture and Regeneration Research Group, School of Life Sciences, University of Westminster, London, W1W 6UW, UK. Electronic address:

Peptidylarginine deiminases (PADs) are phylogenetically conserved calcium-dependent enzymes which post-translationally convert arginine into citrulline in target proteins in an irreversible manner, leading to functional and structural changes in target proteins. Protein deimination can cause the generation of neo-epitopes, affect gene regulation and also allow for protein moonlighting and therefore facilitate multifaceted functions of the same protein. PADs are furthermore a key regulator of cellular release of extracellular vesicle (EVs), which are found in most body fluids and participate in cellular communication via transfer of cargo proteins and genetic material. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.biochi.2020.02.017DOI Listing
February 2020