741 results match your criteria Biochimica et biophysica acta. Molecular cell research[Journal]


Human Mpv17-like protein with a mitigating effect on mtDNA damage is involved in cAMP/PKA signaling in the mitochondrial matrix.

Biochim Biophys Acta Mol Cell Res 2020 Jul 1:118792. Epub 2020 Jul 1.

Life Science Innovation Center, University of Fukui, Fukui 910-1193, Japan.

Human Mpv17-like protein (M-LPH/Mpv17L) is thought to play a role in minimizing mitochondrial dysfunction caused by mitochondrial DNA (mtDNA) damage. We have recently demonstrated that, in addition to an increase of mtDNA damage, M-LPH-knockout (M-LPH-KO) in HepG2 cells causes a significant reduction of mitochondrial transcription factor A (TFAM) protein, an essential factor for mtDNA maintenance, along with an increase in its phosphorylation. These intracellular changes suggested an association of M-LPH with the cAMP/PKA signaling pathway, as selective degradation of TFAM by mitochondrial protease is driven by protein kinase A (PKA)-dependent phosphorylation. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbamcr.2020.118792DOI Listing

Eif2s3y regulates the proliferation of spermatogonial stem cells via Wnt6/-catenin signaling pathway.

Biochim Biophys Acta Mol Cell Res 2020 Jul 1:118790. Epub 2020 Jul 1.

College of Veterinary Medicine, Shaanxi Center of Stem Cells Engineering & Technology, Northwest A & F University, Yangling, Shaanxi, China. Electronic address:

Eukaryotic translation initiation factor 2 subunit 3 and structural gene Y-linked (Eif2s3y) gene, the gene encoding eIF2γ protein, is globally expressed in all tissues and plays important roles in regulating global and gene-specific mRNA translation initiation. It has been noticed that Eif2s3y plays crucial roles in spermatogenesis, however, the mechanism remains unclear. In the current study, transgenic Eif2s3y mice were generated to test our hypothesis that the Eif2s3y promotes the proliferation of spermatogonial stem cells (SSCs). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbamcr.2020.118790DOI Listing

Ryanodine receptor 1 mediated dexamethasone-induced chondrodysplasia in fetal rats.

Biochim Biophys Acta Mol Cell Res 2020 Jun 30:118791. Epub 2020 Jun 30.

Department of Pharmacology, Basic Medical School of Wuhan University, Wuhan 430071, China; Hubei Provincial Key Laboratory of Developmentally Originated Disease, Wuhan, 430071, China. Electronic address:

Background: Osteoarthritis is caused by cartilage dysplasia and has fetal origin. Prenatal dexamethasone exposure (PDE) induced chondrodysplasia in fetal rats by inhibiting transforming growth factor β (TGFβ) signaling. This study aimed to determine the effect of dexamethasone on fetal cartilage development and illustrate the underlying molecular mechanism. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbamcr.2020.118791DOI Listing

Role of calpains in promoting desmin filaments depolymerization and muscle atrophy.

Authors:
Shenhav Cohen

Biochim Biophys Acta Mol Cell Res 2020 Jun 27;1867(10):118788. Epub 2020 Jun 27.

Technion Israel Institute of Technology, Israel. Electronic address:

Muscle atrophy is an inevitable sequel of fasting, denervation, aging, exposure to microgravity, and many human diseases including, cancer, type-2 diabetes, and renal failure. During atrophy the destruction of the muscle's fundamental contractile machinery, the myofibrils, is accelerated leading to a reduction in muscle mass, weakness, frailty, and physical disability. Recent findings indicate that atrophy can be a major cause of death in affected individuals, and inhibition of muscle wasting is likely to prolong survival. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbamcr.2020.118788DOI Listing

JAK2 regulates Nav1.6 channel function via FGF14 phosphorylation.

Biochim Biophys Acta Mol Cell Res 2020 Jun 26:118786. Epub 2020 Jun 26.

Department of Pharmacology & Toxicology, The University of Texas Medical Branch, Galveston, TX, USA; Center for Addiction Research, The University of Texas Medical Branch, Galveston, TX, USA. Electronic address:

Background: Protein interactions between voltage-gated sodium (Nav) channels and accessory proteins play an essential role in neuronal firing and plasticity. However, a surprisingly limited number of kinases have been identified as regulators of these molecular complexes. We hypothesized that numerous as-of-yet unidentified kinases indirectly regulate the Nav channel via modulation of the intracellular fibroblast growth factor 14 (FGF14), an accessory protein with numerous unexplored phosphomotifs and required for channel function in neurons. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbamcr.2020.118786DOI Listing

Kinetic and protective role of autophagy in manganese-exposed BV-2 cells.

Biochim Biophys Acta Mol Cell Res 2020 Jun 24:118787. Epub 2020 Jun 24.

CONICET, Universidad de Buenos Aires, Instituto de Química Biológica Ciencias Exactas y Naturales (IQUIBICEN), Facultad de Ciencias Exactas y Naturales, Departamento de Química Biológica. Pabellón II, Ciudad Universitaria, 1428 Buenos Aires, Argentina. Electronic address:

Manganese (Mn) plays an important role in many physiological processes. Nevertheless, Mn accumulation in the brain can cause a parkinsonian-like syndrome known as manganism. Unfortunately, the therapeutic options for this disease are scarce and of limited efficacy. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbamcr.2020.118787DOI Listing

Impact of intercellular connectivity on epithelial mesenchymal transition plasticity.

Biochim Biophys Acta Mol Cell Res 2020 Jun 23:118784. Epub 2020 Jun 23.

School of Medical Science and Technology, Indian Institute of Technology Kharagpur, 721302, West Bengal, India.

Epithelial mesenchymal transition (EMT) in development, tissue repair and carcinogenesis involves cellular plasticity with varying degrees of epithelial and mesenchymal properties. Several recent studies have focused on EMT phenotypic dynamism; however, information on cellular interaction in the context of EMT is inadequate. In our previous study, we investigated EMT phenotypic plasticity and anticipated it as a population driven interactive process. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbamcr.2020.118784DOI Listing

LGALS1 acts as a pro-survival molecule in AML.

Biochim Biophys Acta Mol Cell Res 2020 Jun 23;1867(10):118785. Epub 2020 Jun 23.

Department of Leukemia, University of Texas MD Anderson Cancer Center, Houston, TX 77030, United States of America; Section of Molecular Hematology and Therapy, University of Texas MD Anderson Cancer Center, Houston, TX 77030, United States of America.

The galectin LGALS1 is a glycan binding protein that regulates intracellular (e.g. signal transduction) and extracellular processes (e. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbamcr.2020.118785DOI Listing

NMDA-induced nitric oxide generation and CREB activation in central nervous system is dependent on eukaryotic elongation factor 2 kinase.

Biochim Biophys Acta Mol Cell Res 2020 Jun 20;1867(10):118783. Epub 2020 Jun 20.

Program of Neurosciences, Institute of Biology, Fluminense Federal University, Niterói, RJ, Brazil; Department of Physiology and Pharmacology, Biomedical Institute, Fluminense Federal University, Niterói, RJ, Brazil. Electronic address:

The NMDA receptor is crucial to several functions in CNS physiology and some of its effects are mediated by promoting nitric oxide production from L-arginine and activation of signaling pathways and the transcription factor CREB. Our previous work demonstrated in retinal cells that increasing intracellular free L-arginine levels directly correlates to nitric oxide (NO) generation and can be promoted by protein synthesis inhibition and increase of free L-arginine concentration. Eukaryotic elongation factor 2 kinase (eEF2K), a calcium/calmodulin-dependent kinase, is also known to be activated by NMDA receptors leading to protein synthesis inhibition. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbamcr.2020.118783DOI Listing

Epithelial-to-mesenchymal transition as the driver of changing carcinoma and glioblastoma microenvironment.

Biochim Biophys Acta Mol Cell Res 2020 Jun 15;1867(10):118782. Epub 2020 Jun 15.

Department of Genetic Toxicology and Cancer Biology, National Institute of Biology, Večna pot 111, 1000 Ljubljana, Slovenia; Jožef Stefan International Postgraduate School, Jamova cesta 39, 1000 Ljubljana, Slovenia; Faculty of Chemistry and Chemical Technology, Večna pot 113, 1000 Ljubljana, Slovenia. Electronic address:

Epithelial-to-mesenchymal transition (EMT) is an essential molecular and cellular process that is part of normal embryogenesis and wound healing, and also has a ubiquitous role in various types of carcinoma and glioblastoma. EMT is activated and regulated by specific microenvironmental endogenous triggers and a complex network of signalling pathways. These mostly include epigenetic events that affect protein translation-controlling factors and proteases, altogether orchestrated by the switching on and off of oncogenes and tumour-suppressor genes in cancer cells. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbamcr.2020.118782DOI Listing

Leading the invasion: The role of Cathepsin S in the tumour microenvironment.

Biochim Biophys Acta Mol Cell Res 2020 Jun 13;1867(10):118781. Epub 2020 Jun 13.

The Patrick G Johnston Centre for Cancer Research, Medical Biology Centre, Queen's University Belfast, 97 Lisburn Road, Belfast BT9 7AE, UK. Electronic address:

Elevated expression of the cysteine protease Cathepsin S has been correlated with a number of different cancer types in recent years. As tools have been developed to enable more accurate examination of individual cathepsin species, our knowledge and appreciation of the role that this protease plays in facilitating cancer has increased exponentially. This review focuses on our current understanding of the role of Cathepsin S within tumours and the surrounding microenvironment. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbamcr.2020.118781DOI Listing

GSK-3 and miRs: Master regulators of therapeutic sensitivity of cancer cells.

Biochim Biophys Acta Mol Cell Res 2020 Jun 7;1867(10):118770. Epub 2020 Jun 7.

Department of Microbiology and Immunology, Brody School of Medicine, East Carolina University, Greenville, NC 27858, USA; Department of Microbiology and Immunology, Brody School of Medicine, East Carolina University, Brody Building 5N98C, Greenville, NC 27858, USA. Electronic address:

Glycogen synthetase kinase-3 (GSK-3) and microRNAs (miRs) affect many critical signaling pathways important in cell growth. GSK-3 is a serine/threonine (S/T) protein kinase. Often when GSK-3 phosphorylates other proteins, they are inactivated and the signaling pathway is shut down. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbamcr.2020.118770DOI Listing

GSK-3-associated signaling is crucial to virus infection of cells.

Biochim Biophys Acta Mol Cell Res 2020 Jun 6;1867(10):118767. Epub 2020 Jun 6.

Department of Microbiology and Immunology, Brody School of Medicine, East Carolina University, Greenville, NC 27858, USA. Electronic address:

Signal transduction pathways play important roles in virus infection, replication, and associated pathogenesis. Some of the best understood cell signaling networks are crucial to virus infections such the mitogen-activated protein kinase (MAPK), phosphoinositide 3-kinase (PI3K), protein kinase C (PKC), and the WNT/β-catenin pathways. Glycogen synthase kinase-3 (GSK-3) is a lesser known signaling molecule in the field of virus research. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbamcr.2020.118767DOI Listing

Glycogen synthase kinase (GSK)-3 and the double-strand RNA-dependent kinase, PKR: When two kinases for the common good turn bad.

Biochim Biophys Acta Mol Cell Res 2020 Jun 5;1867(10):118769. Epub 2020 Jun 5.

Istituto di Genetica Molecolare "Luigi Luca Cavalli-Sforza", Consiglio Nazionale delle Ricerche (IGM-CNR), Bologna, Italy; IRCCS, Istituto Ortopedico Rizzoli, Bologna, Italy. Electronic address:

Glycogen synthase kinase (GSK)-3α/β and the double-stranded RNA-dependent kinase PKR are two sentinel kinases that carry-out multiple similar yet distinct functions in both the cytosol and the nucleus. While these kinases belong to separate signal transduction cascades, they demonstrate an uncanny propensity to regulate many of the same proteins either through direct phosphorylation or by altering transcription/translation, including: c-MYC, NF-κB, p53 and TAU, as well as each another. A significant number of studies centered on the GSK3 kinases have led to the identification of the GSK3 interactome and a number of substrates, which link GSK3 activity to metabolic control, translation, RNA splicing, ribosome biogenesis, cellular division, DNA repair and stress/inflammatory signaling. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbamcr.2020.118769DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7273171PMC

Amlexanox exerts anti-inflammatory actions by targeting phosphodiesterase 4B in lipopolysaccharide-activated macrophages.

Biochim Biophys Acta Mol Cell Res 2020 Jun 3:118766. Epub 2020 Jun 3.

Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China. Electronic address:

Amlexanox, an anti-inflammatory agent, is widely used for treating aphthous ulcers. Recently, amlexanox has received considerable attention because of its efficacy in mitigating metabolic inflammation via directly suppressing IKKε/TBK1. However, because the knockdown of IKKε/TBK1 has no anti-inflammatory effect on lipopolysaccharide (LPS)-primed RAW264. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbamcr.2020.118766DOI Listing

The E3 ubiquitin ligase Slimb/β-TrCP is required for normal copper homeostasis in Drosophila.

Biochim Biophys Acta Mol Cell Res 2020 Jun 2:118768. Epub 2020 Jun 2.

School of Biological Sciences, Monash University, Wellington Rd, Clayton 3800, VIC, Australia. Electronic address:

The Drosophila Slimb (Slmb) gene encodes a Skp1-Cul1-F-box (SCP) E3 ubiquitin ligase orthologous to the human β-TrCP/BTRC protein. Slmb and/or BTRC play regulatory roles in numerous biological processes by ubiquitinating several substrate proteins which are then targeted for proteasomal degradation. Here, we demonstrate an additional role for Slmb in maintaining cellular copper homeostasis. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbamcr.2020.118768DOI Listing

Src couples estrogen receptor to the anticipatory unfolded protein response and regulates cancer cell fate under stress.

Biochim Biophys Acta Mol Cell Res 2020 Jun 2:118765. Epub 2020 Jun 2.

Department of Biochemistry, University of Illinois, Urbana, IL, USA; Cancer Center at Illinois, University of Illinois, Urbana, IL, USA. Electronic address:

Accumulation of unfolded protein, or other stresses, activates the classical reactive unfolded protein response (UPR). In the recently characterized anticipatory UPR, receptor-bound estrogen, progesterone and other mitogenic hormones rapidly elicit phosphorylation of phospholipase C γ (PLCγ), activating the anticipatory UPR. How estrogen and progesterone activating their receptors couples to PLCγ phosphorylation and anticipatory UPR activation was unknown. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbamcr.2020.118765DOI Listing

NMK-BH2, a novel microtubule-depolymerising bis (indolyl)-hydrazide-hydrazone, induces apoptotic and autophagic cell death in cervical cancer cells by binding to tubulin at colchicine - site.

Biochim Biophys Acta Mol Cell Res 2020 Jun 2;1867(10):118762. Epub 2020 Jun 2.

Department of Biotechnology, Dr. B.C. Guha Centre for Genetic Engineering and Biotechnology, University of Calcutta, 35 Ballygunge Circular Road, Kolkata 700019, West Bengal, India. Electronic address:

Background: Microtubules, the key components of the eukaryotic cytoskeleton and mitotic spindle, are one of the most sought-after targets for cancer chemotherapy, especially due to their indispensible role in mitosis. Cervical cancer is a prevalent malignancy among women of developing countries including India. In spite of the remarkable therapeutic advancement, the non-specificity of chemotherapeutic drugs adversely affect the patients' survival and well-being, thus, necessitating the quest for novel indole-based anti-microtubule agent against cervical cancer, with high degree of potency and selectivity. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbamcr.2020.118762DOI Listing

Expression of nuclear XIAP associates with cell growth and drug resistance and confers poor prognosis in breast cancer.

Biochim Biophys Acta Mol Cell Res 2020 May 30:118761. Epub 2020 May 30.

Laboratório de Hemato-Oncologia Celular e Molecular, Programa de Hemato-Oncologia Molecular, Instituto Nacional de Câncer (INCA), Praça da Cruz Vermelha, 23, 6° andar, Centro, 20 230 130 Rio de Janeiro, RJ, Brazil. Electronic address:

Evasion from apoptosis is one of the hallmarks of cancer. X-linked inhibitor of apoptosis protein (XIAP) is known to modulate apoptosis by inhibiting caspases and ubiquitinating target proteins. XIAP is mainly found at the cytoplasm, but recent data link nuclear XIAP to poor prognosis in breast cancer. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbamcr.2020.118761DOI Listing

Hypomorphic CAMKK2 in EA.hy926 endothelial cells causes abnormal transferrin trafficking, iron homeostasis and glucose metabolism.

Biochim Biophys Acta Mol Cell Res 2020 May 30:118763. Epub 2020 May 30.

Canadian Centre for Agri-Food Research in Health and Medicine, St. Boniface Hospital Research Centre, Winnipeg, MB, R2H 2A6, Canada; Department of Physiology and Pathophysiology, University of Manitoba, Winnipeg, MB, R3E 0J9, Canada.

We recently reported that loss of calcium/calmodulin-dependent protein kinase kinase-2 (CAMKK2), a serine/threonine kinase activated by intracellular calcium, in mice leads to tissue-specific aberrant turnover of transferrin (TF), a receptor-mediated iron-transporter that supplies iron to tissues. Iron dyshomeostasis is associated with the pathogenesis of several diseases, making TF transport relevant to health. In this study, we used hemizygous CAMKK2 hypomorphic human endothelial cell (EA. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbamcr.2020.118763DOI Listing

Glycogen synthase kinase-3 signaling in cellular and behavioral responses to psychostimulant drugs.

Biochim Biophys Acta Mol Cell Res 2020 Sep 23;1867(9):118746. Epub 2020 May 23.

Center for Substance Abuse Research, Lewis Katz School of Medicine, Temple University, Philadelphia, PA 19140, USA; Department of Pharmacology, Lewis Katz School of Medicine, Temple University, Philadelphia, PA 19140, USA. Electronic address:

Glycogen synthase kinase-3 (GSK-3) is a serine/threonine kinase implicated in numerous physiological processes and cellular functions through its ability to regulate the function of many proteins, including transcription factors and structural proteins. GSK-3β has been demonstrated to function as a regulator of multiple behavioral processes induced by drugs of abuse, particularly psychostimulant drugs. In this review, we provide an overview of the regulation of GSK-3β activity produced by psychostimulants, and the role of GSK-3β signaling in psychostimulant-induced behaviors including drug reward, associative learning and memory which play a role in the maintenance of drug-seeking. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbamcr.2020.118746DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7313643PMC
September 2020

Control of neuronal excitability by GSK-3beta: Epilepsy and beyond.

Authors:
Tomasz Jaworski

Biochim Biophys Acta Mol Cell Res 2020 Sep 23;1867(9):118745. Epub 2020 May 23.

Laboratory of Animal Models, Nencki Institute of Experimental Biology, Warsaw, Poland. Electronic address:

Glycogen synthase kinase 3beta (GSK-3β) is an enzyme with a variety of cellular functions in addition to the regulation of glycogen metabolism. In the central nervous system, different intracellular signaling pathways converge on GSK-3β through a cascade of phosphorylation events that ultimately control a broad range of neuronal functions in the development and adulthood. In mice, genetically removing or increasing GSK-3β cause distinct functional and structural neuronal phenotypes and consequently affect cognition. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbamcr.2020.118745DOI Listing
September 2020

Complementation studies with human ClpP in Bacillus subtilis.

Biochim Biophys Acta Mol Cell Res 2020 Sep 20;1867(9):118744. Epub 2020 May 20.

Institute of Microbiology of the Department of Biology, University of Greifswald, Germany. Electronic address:

ATP-dependent intracellular proteolysis is essential for all living organisms. ClpP, the proteolytic subunit of the ATP-dependent Clp proteases, shares 56% protein identity between B. subtilis and man. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbamcr.2020.118744DOI Listing
September 2020

Loss of tumor susceptibility gene 101 (TSG101) perturbs endoplasmic reticulum structure and function.

Biochim Biophys Acta Mol Cell Res 2020 Sep 15;1867(9):118741. Epub 2020 May 15.

Biophysics & Structural Genomics Division, Saha Institute of Nuclear Physics, 1/AF Bidhannagar, Kolkata 700064, India; Homi Bhabha National Institute, India. Electronic address:

Tumor susceptibility gene 101 (TSG101), an ESCRT-I protein, is implicated in multiple cellular processes and its functional depletion can lead to blocked lysosomal degradation, cell cycle arrest, demyelination and neurodegeneration. Here, we show that loss of TSG101 results in endoplasmic reticulum (ER) stress and this causes ER membrane remodelling (EMR). This correlates with an expansion of ER, increased vacuolation, altered relative distribution of the rough and smooth ER and disruption of three-way junctions. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbamcr.2020.118741DOI Listing
September 2020
5.019 Impact Factor

Pontin-deficiency causes senescence in fibroblast cells and epidermal keratinocytes but induces apoptosis in cancer cells.

Biochim Biophys Acta Mol Cell Res 2020 Sep 14;1867(9):118740. Epub 2020 May 14.

Department of Biological Sciences, Sookmyung Women's University, Seoul 04310, Republic of Korea; Research Institute of Women's Health, Sookmyung Women's University, Seoul 04310, Republic of Korea. Electronic address:

Pontin, a member of the AAA+ ATPase family, plays important roles in a variety of cellular processes, including transcription regulation, DNA damage response, telomerase activity, and cellular transformation. In the previous studies, Pontin deletion in mice was lethal to embryos. Here, we demonstrate that the depletion of Pontin induced cellular senescence in mouse and human fibroblasts as well as in mouse epidermal keratinocytes. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbamcr.2020.118740DOI Listing
September 2020

GSK-3 in liver diseases: Friend or foe?

Biochim Biophys Acta Mol Cell Res 2020 Sep 15;1867(9):118743. Epub 2020 May 15.

Institute for Biomedical Research and Innovation, National Research Council (CNR), Palermo, Italy. Electronic address:

Liver diseases, including hepatitis due to hepatitis B or C virus infection, non-alcoholic fatty liver disease, and hepatocellular carcinoma pose major challenges for overall health due to limited curative treatment options. Thus, there is an urgent need to develop new therapeutic strategies for the treatment of these diseases. A better understanding of the signaling pathways involved in the pathogenesis of liver diseases can help to improve the efficacy of emerging therapies, mainly based on pharmacological approaches, which influence one or more specific molecules involved in key signal transduction pathways. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbamcr.2020.118743DOI Listing
September 2020

Role of damage and management in muscle hypertrophy: Different behaviors of muscle stem cells in regeneration and hypertrophy.

Biochim Biophys Acta Mol Cell Res 2020 Sep 14;1867(9):118742. Epub 2020 May 14.

Institut National de la Santé et de la Recherche Médicale U1016, Institut Cochin, Paris, France.

Skeletal muscle is a dynamic tissue with two unique abilities; one is its excellent regenerative ability, due to the activity of skeletal muscle-resident stem cells named muscle satellite cells (MuSCs); and the other is the adaptation of myofiber size in response to external stimulation, intrinsic factors, or physical activity, which is known as plasticity. Low physical activity and some disease conditions lead to the reduction of myofiber size, called atrophy, whereas hypertrophy refers to the increase in myofiber size induced by high physical activity or anabolic hormones/drugs. MuSCs are essential for generating new myofibers during regeneration and the increase in new myonuclei during hypertrophy; however, there has been little investigation of the molecular mechanisms underlying MuSC activation, proliferation, and differentiation during hypertrophy compared to those of regeneration. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbamcr.2020.118742DOI Listing
September 2020

Role of the HIF oxygen sensing pathway in cell defense and proliferation through the control of amino acid metabolism.

Biochim Biophys Acta Mol Cell Res 2020 Sep 13;1867(9):118733. Epub 2020 May 13.

Research Unit, Hospital of Santa Cristina, Research Institute Princesa (IP), Autonomous University of Madrid, Madrid 28009, Spain; CIBER de Enfermedades Cardiovasculares, Carlos III Health Institute, Madrid, Spain. Electronic address:

Cell responses to reduced oxygen supply (hypoxia) are largely mediated by hypoxia-inducible transcription factors (HIFs). The pathophysiological role of the HIF pathway is driven by its ability to potentiate key biological processes as part of the adaptation to hypoxia, such as erythropoiesis and angiogenesis. Moreover, the role of HIF signaling in the reprogramming of cell metabolism is also critical to understand the role of these transcription factors in health and disease. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbamcr.2020.118733DOI Listing
September 2020

Corrigendum to "Suppression of androgen receptor enhances the self-renewal of mesenchymal stem cells through elevated expression of EGFR" [Biochim. Biophys. Acta. 2013 May; 1833 (5): 1222-34].

Biochim Biophys Acta Mol Cell Res 2020 Aug 11;1867(8):118730. Epub 2020 May 11.

George Whipple Lab for Cancer Research, Department of Apthology, Urology, and Radiation Oncology and the Wilmot Cancer Center, University of Rochester Medical Center, Rochester, NY, USA; Sex Hormone Research Center, China Medical University and Hospital, Taichung, Taiwan. Electronic address:

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbamcr.2020.118730DOI Listing

Brain-derived neurotrophic factor (BDNF) promotes molecular polarization and differentiation of immature neuroblastoma cells into definitive neurons.

Biochim Biophys Acta Mol Cell Res 2020 Sep 8;1867(9):118737. Epub 2020 May 8.

Department of Experimental Neurobiology, National Institute of Mental Health, Klecany, Czech Republic; Department of Psychiatry and Medical Psychology, 3rd Faculty of Medicine of Charles University, Prague, Czech Republic. Electronic address:

Throughout development, neuronal progenitors undergo complex transformation into polarized nerve cells, warranting the directional flow of information in the neural grid. The majority of neuronal polarization studies have been carried out on rodent-derived precursor cells, programmed to develop into neurons. Unlike rodent neuronal cells, SH-SY5Y cells derived from human bone marrow present a sub-clone of neuroblastoma line, with their transformation into neuron-like cells showing a range of highly instructive neurobiological characteristics. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbamcr.2020.118737DOI Listing
September 2020

The cross-talk of NOTCH and GSK-3 signaling in colon and other cancers.

Authors:
Fred E Bertrand

Biochim Biophys Acta Mol Cell Res 2020 Sep 8;1867(9):118738. Epub 2020 May 8.

Department of Nutrition Sciences, School of Health Professions, University of Alabama at Birmingham, Birmingham, AL 35294, USA. Electronic address:

The GSK-3 kinases, GSK-3α and GSK-3β, have a central role in regulating multiple cellular processes such as glycogen synthesis, insulin signaling, cell proliferation and apoptosis. GSK-3β is the most well studied, and was originally described for its role in regulating glycogen synthase. GSK-3β has been studied as a participant in the oncogenic process in a variety of cancers due to its intersection with the PTEN/PI3K/AKT and RAS/RAF/MEK/ERK pathways. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbamcr.2020.118738DOI Listing
September 2020

Correcting an instance of synthetic lethality with a pro-survival sequence.

Biochim Biophys Acta Mol Cell Res 2020 Sep 7;1867(9):118734. Epub 2020 May 7.

Department of Chemistry and Chemical Engineering, Royal Military College, Kingston, Ontario, Canada. Electronic address:

A human cDNA encoding the LIM domain containing 194 amino acid cysteine and glycine rich protein 3 (CSRP3) was identified as a BAX suppressor in yeast and a pro-survival sequence that abrogated copper mediated regulated cell death (RCD). Yeast lacks a CSRP3 orthologue but it has four LIM sequences, namely RGA1, RGA2, LRG1 and PXL1. These are known regulators of stress responses yet their roles in RCD remain unknown. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbamcr.2020.118734DOI Listing
September 2020

Cooperative roles of PAK1 and filamin A in regulation of vimentin assembly and cell extension formation.

Biochim Biophys Acta Mol Cell Res 2020 Sep 7;1867(9):118739. Epub 2020 May 7.

Faculty of Dentistry, University of Toronto, Toronto, ON M5G 1G6, Canada. Electronic address:

The formation of extensions in cell migration requires tightly coordinated reorganization of all three cytoskeletal polymers but the mechanisms by which intermediate filament networks interact with actin to generate extensions are not well-defined. We examined interactions of the actin binding protein filamin A (FLNA) with vimentin in extension formation by fibroblasts. Knockdown (KD) of vimentin in fibroblasts reduced the lengths of cell extensions by 50% (p < 0. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbamcr.2020.118739DOI Listing
September 2020

Decrease of Rab11 prevents the correct dendritic arborization, synaptic plasticity and spatial memory formation.

Biochim Biophys Acta Mol Cell Res 2020 Sep 7;1867(9):118735. Epub 2020 May 7.

Universidad Nacional de Córdoba (UNC), Av. Haya de la Torre s/n Ciudad Universitaria, 5000 Córdoba, Argentina; Instituto de Investigación Médica Mercedes y Martıín Ferreyra (INIMEC-CONICET-UNC), Av. Friuli 2434, 5016 Córdoba, Argentina. Electronic address:

Emerging evidence shows that Rab11 recycling endosomes (REs Rab11) are essential for several neuronal processes, including the proper functioning of growth cones, synapse architecture regulation and neuronal migration. However, several aspects of REs Rab11 remain unclear, such as its sub-cellular distribution across neuronal development, contribution to dendritic tree organization and its consequences in memory formation. In this work we show a spatio-temporal correlation between the endogenous localization of REs Rab11 and developmental stage of neurons. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbamcr.2020.118735DOI Listing
September 2020
5.019 Impact Factor

Deciphering a distinct regulatory network of TEAD4, CDX2 and GATA3 in humans for trophoblast transition from embryonic stem cells.

Biochim Biophys Acta Mol Cell Res 2020 Sep 7;1867(9):118736. Epub 2020 May 7.

Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, China; Research Centre for Women's and Infants' Health, Lunenfeld-Tanenbaum Research Institute, Sinai Health System, Toronto, M5T 3H7, Canada. Electronic address:

The placenta is an essential organ for the fetus, but its regulatory mechanism for formation of functional trophoblast lineage remains elusive in humans. Although widely known in mice, TEAD4 and its downstream targets CDX2 and GATA3 have not been determined in human models. In this work, we used a human model of trophoblast transition from BAP (BMP4, A83-01 and PD173074)-treated human embryonic stem cells (hESCs) and performed multiple gain- and loss-of-function tests of TEAD4, CDX2 or GATA3 to study their roles during this process. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbamcr.2020.118736DOI Listing
September 2020

Deregulated PTEN/PI3K/AKT/mTOR signaling in prostate cancer: Still a potential druggable target?

Biochim Biophys Acta Mol Cell Res 2020 Sep 29;1867(9):118731. Epub 2020 Apr 29.

Department of Biomedical, Metabolic, and Neurological Sciences, University of Modena and Reggio Emilia, 41124 Modena, MO, Italy. Electronic address:

Although the prognosis of patients with localized prostate cancer is good after surgery, with a favorable response to androgen deprivation therapy, about one third of them invariably relapse, and progress to castration-resistant prostate cancer. Overall, prostate cancer therapies remain scarcely effective, thus it is mandatory to devise alternative treatments enhancing the efficacy of surgical castration and hormone administration. Dysregulation of the phosphoinositide 3-kinase pathway has attracted growing attention in prostate cancer due to the highly frequent association of epigenetic and post-translational modifications as well as to genetic alterations of both phosphoinositide 3-kinase and PTEN to onset and/or progression of this malignancy, and to resistance to canonical androgen-deprivation therapy. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbamcr.2020.118731DOI Listing
September 2020

Protein synthesis inhibition promotes nitric oxide generation and activation of CGKII-dependent downstream signaling pathways in the retina.

Biochim Biophys Acta Mol Cell Res 2020 Aug 29;1867(8):118732. Epub 2020 Apr 29.

Program of Neurosciences, Institute of Biology, Fluminense Federal University, Niterói, RJ, Brazil; Department of Neurobiology, Institute of Biology, Fluminense Federal University, Niterói, RJ, Brazil. Electronic address:

Nitric oxide is an important neuromodulator in the CNS, and its production within neurons is modulated by NMDA receptors and requires a fine-tuned availability of L-arginine. We have previously shown that globally inhibiting protein synthesis mobilizes intracellular L-arginine "pools" in retinal neurons, which concomitantly enhances neuronal nitric oxide synthase-mediated nitric oxide production. Activation of NMDA receptors also induces local inhibition of protein synthesis and L-arginine intracellular accumulation through calcium influx and stimulation of eucariotic elongation factor type 2 kinase. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbamcr.2020.118732DOI Listing

Hsp90 facilitates acquired drug resistance of tumor cells through cholesterol modulation however independent of tumor progression.

Biochim Biophys Acta Mol Cell Res 2020 Aug 25;1867(8):118728. Epub 2020 Apr 25.

CSIR-Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, Telangana, India. Electronic address:

Acquired multidrug resistance of cancer cells challenges the chemotherapeutic interventions. To understand the role of molecular chaperone, Hsp90 in drug adapted tumor cells, we have used in vitro drug adapted epidermoid tumor cells as a model system. We found that chemotherapeutic drug adaptation of tumor cells is mediated by induced activities of both Hsp90 and P-glycoprotein (P-gp). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbamcr.2020.118728DOI Listing

Overexpression of budding yeast protein phosphatase Ppz1 impairs translation.

Biochim Biophys Acta Mol Cell Res 2020 Aug 24;1867(8):118727. Epub 2020 Apr 24.

Institut de Biotecnologia i Biomedicina, Universitat Autònoma de Barcelona, Cerdanyola del Vallès, Catalonia, Spain; Departament de Bioquímica i Biologia Molecular, Facultat de Veterinària, Universitat Autònoma de Barcelona, Cerdanyola del Vallès, Catalonia, Spain. Electronic address:

The Ser/Thr protein phosphatase Ppz1 from Saccharomyces cerevisiae is the best characterized member of a family of enzymes only found in fungi. Ppz1 is regulated in vivo by two inhibitory subunits, Hal3 and Vhs3, which are moonlighting proteins also involved in the decarboxylation of the 4-phosphopantothenoylcysteine (PPC) intermediate required for coenzyme A biosynthesis. It has been reported that, when overexpressed, Ppz1 is the most toxic protein in yeast. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbamcr.2020.118727DOI Listing

Ubiquitin-dependent proteasomal degradation of AMPK gamma subunit by Cereblon inhibits AMPK activity.

Biochim Biophys Acta Mol Cell Res 2020 Aug 22;1867(8):118729. Epub 2020 Apr 22.

Department of Life Science and Environmental Biochemistry, Pusan National University, Miryang 50463, Republic of Korea. Electronic address:

Cereblon (CRBN), a substrate receptor for Cullin-ring E3 ubiquitin ligase (CRL), is a major target protein of immunomodulatory drugs. An earlier study demonstrated that CRBN directly interacts with the catalytic α subunit of AMP-activated protein kinase (AMPK), a master regulator of energy homeostasis, down-regulating the enzymatic activity of AMPK. However, it is not clear how CRBN modulates AMPK activity. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbamcr.2020.118729DOI Listing

Apparent stiffness of vimentin intermediate filaments in living cells and its relation with other cytoskeletal polymers.

Biochim Biophys Acta Mol Cell Res 2020 Aug 19;1867(8):118726. Epub 2020 Apr 19.

Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Química Biológica, Instituto de Química Biológica (IQUIBICEN), Ciudad de Buenos Aires, Argentina. Electronic address:

The cytoskeleton is a complex network of interconnected biopolymers intimately involved in the generation and transmission of forces. Several mechanical properties of microtubules and actin filaments have been extensively explored in cells. In contrast, intermediate filaments (IFs) received comparatively less attention despite their central role in defining cell shape, motility and adhesion during physiological processes as well as in tumor progression. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbamcr.2020.118726DOI Listing

Metabotropic signaling cascade involved in α4β2 nicotinic acetylcholine receptor-mediated PKCβII activation.

Biochim Biophys Acta Mol Cell Res 2020 Aug 15;1867(8):118721. Epub 2020 Apr 15.

Department of Pharmacology, College of Pharmacy, Chonnam National University, Gwang-Ju 61186, Republic of Korea. Electronic address:

Nicotinic acetylcholine receptors (nAChRs) belong to the ionophore receptor family, which regulates plasma membrane conductance to Na, K, and Ca ions. Some studies, however, have shown that nAChRs also employ second messengers for intracellular signaling. We previously showed that α4β2 nAChR mediates the translocation of protein kinase CβII (PKCβII) from the cytoplasm to the plasma membrane, which is a typical activation marker for PKCβII. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbamcr.2020.118721DOI Listing

Corrigendum to "Poly(ADP-ribose) polymerase 1 inhibition protects against low shear stress induced inflammation" [BBA-Mol Cell Res. 1833/1 January 2013 Pages 59-68].

Biochim Biophys Acta Mol Cell Res 2020 Jul 15;1867(7):118706. Epub 2020 Apr 15.

The Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and Chinese Ministry of Public Health, Qilu Hospital of Shandong University, Jinan, Shandong, China. Electronic address:

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbamcr.2020.118706DOI Listing

Functional association of the stress-responsive LiaH protein and the minimal TatAyCy protein translocase in Bacillus subtilis.

Biochim Biophys Acta Mol Cell Res 2020 Aug 14;1867(8):118719. Epub 2020 Apr 14.

University of Groningen, University Medical Center Groningen, Department of Medical Microbiology, Hanzeplein 1, P.O. box 30001, 9700, RB, Groningen, the Netherlands. Electronic address:

The bacterial twin-arginine (Tat) pathway serves in the exclusive secretion of folded proteins with bound cofactors. While Tat pathways in Gram-negative bacteria and chloroplast thylakoids consist of conserved TatA, TatB and TatC subunits, the Tat pathways of Bacillus species and many other Gram-positive bacteria stand out for their minimalist nature with the core translocase being composed of essential TatA and TatC subunits only. Here we addressed the question whether the minimal TatAyCy translocase of Bacillus subtilis recruits additional cellular components that modulate its activity. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbamcr.2020.118719DOI Listing

Interferon-γ and high glucose-induced opening of Cx43 hemichannels causes endothelial cell dysfunction and damage.

Biochim Biophys Acta Mol Cell Res 2020 Aug 14;1867(8):118720. Epub 2020 Apr 14.

Departamento de Neurología, Escuela de Medicina and Centro Interdisciplinario de Neurociencias, Facultad de Medicina, Pontificia Universidad Católica de Chile, Santiago, Chile. Electronic address:

Both IFN-γ or high glucose have been linked to systemic inflammatory imbalance with serious repercussions not only for endothelial function but also for the formation of the atherosclerotic plaque. Although the uncontrolled opening of connexin hemichannels underpins the progression of various diseases, whether they are implicated in endothelial cell dysfunction and damage evoked by IFN-γ plus high glucose remains to be fully elucidated. In this study, by using live cell imaging and biochemical approaches, we demonstrate that IFN-γ plus high glucose augment endothelial connexin43 hemichannel activity, resulting in the increase of ATP release, ATP-mediated Ca dynamics and production of nitric oxide and superoxide anion, as well as impaired insulin-mediated uptake and intercellular diffusion of glucose and cell survival. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbamcr.2020.118720DOI Listing

Cathepsin C is a novel mediator of podocyte and renal injury induced by hyperglycemia.

Biochim Biophys Acta Mol Cell Res 2020 Aug 14;1867(8):118723. Epub 2020 Apr 14.

Laboratory of Molecular and Cellular Nephrology, Mossakowski Medical Research Centre, Polish Academy of Sciences, Gdansk, Poland; Faculty of Chemistry, University of Gdansk, Poland.

A growing body of evidence suggests a role of proteolytic enzymes in the development of diabetic nephropathy. Cathepsin C (CatC) is a well-known regulator of inflammatory responses, but its involvement in podocyte and renal injury remains obscure. We used Zucker rats, a genetic model of metabolic syndrome and insulin resistance, to determine the presence, quantity, and activity of CatC in the urine. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbamcr.2020.118723DOI Listing
August 2020
5.019 Impact Factor

Impact of intercellular crosstalk between epidermal keratinocytes and dermal fibroblasts on skin homeostasis.

Biochim Biophys Acta Mol Cell Res 2020 Aug 14;1867(8):118722. Epub 2020 Apr 14.

Institute for Pharmacy, Pharmacology and Toxicology, Freie Universität Berlin, Germany; Faculty of Pharmaceutical Sciences, University of British Columbia, Vancouver, BC, Canada. Electronic address:

Dermal fibroblasts seem critical for epidermal maturation and differentiation and recent work demonstrated that diseased fibroblasts may drive pathophysiological processes. Nevertheless, still very little is known about the actual crosstalk between epidermal keratinocytes and dermal fibroblasts and the impact of dermal fibroblasts on epidermal maturation and differentiation. Aiming for a more fundamental understanding of the impact of the cellular crosstalk between keratinocytes and fibroblasts on the skin homeostasis, we generated full-thickness skin equivalents with and without fibroblasts and subsequently analysed them for the expression of skin differentiation markers, their barrier function, skin lipid content and epidermal cell signalling. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbamcr.2020.118722DOI Listing

A novel splice variant of Gαq-coupled Bombyx CAPA-PVK receptor 1 functions as a specific Gαi/o-linked receptor for CAPA-PK.

Biochim Biophys Acta Mol Cell Res 2020 Aug 11;1867(8):118718. Epub 2020 Apr 11.

Institute of Biochemistry, College of Life Sciences, Zijingang Campus, Zhejiang University, Hangzhou, Zhejiang 310058, China. Electronic address:

Alternative splicing enables G protein-coupled receptor (GPCR) genes to greatly increase the number of structurally and functionally distinct receptor isoforms. However, the functional role and relevance of the individual GPCR splice variants in regulating physiological processes are still to be assessed. A naturally occurring alternative splice variant of Bombyx CAPA-PVK receptor, BomCAPA-PVK-R1-Δ341, has been shown to act as a dominant-negative protein to regulate cell surface expression and function of the canonical CAPA-PVK receptor. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbamcr.2020.118718DOI Listing

The secretome of skin cancer cells activates the mTOR/MYC pathway in healthy keratinocytes and induces tumorigenic properties.

Biochim Biophys Acta Mol Cell Res 2020 Aug 10;1867(8):118717. Epub 2020 Apr 10.

Institute of Molecular Animal Breeding and Biotechnology, Gene Center, LMU, München, Germany. Electronic address:

Cutaneous squamous cell carcinoma (cSCC) is the most prominent tumor of non-melanoma skin cancers and the most aggressive tumor among keratinocyte carcinoma of the skin, showing a high potential for local invasion and metastasis. The cSCC incidences increased dramatically in recent years and the disease occurs more commonly than any other malignancy. The secretome of cancer cells is currently the focus of many studies in order to identify new marker proteins for different types of cancer and to investigate its influence on the tumor microenvironment. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbamcr.2020.118717DOI Listing