794 results match your criteria Biochimica et biophysica acta. General subjects[Journal]


The nanoscopic molecular pathway through human skin.

Biochim Biophys Acta Gen Subj 2019 Apr 15. Epub 2019 Apr 15.

Department of Biochemistry and Molecular Biology, University of Southern Denmark, Denmark. Electronic address:

Background: Knowledge regarding the barrier properties of human skin is important for understanding skin pathology, developing of transdermal drug delivery systems and computational skin absorption models; however, the molecular pathways through human skin remains to be fully investigated on a nanoscopic level. In particular the nanoscopic pathway of molecules passing the intercellular lipid bilayers separating the corneocytes in the stratum corneum (SC) is not fully elucidated.

Methods: Using stimulated emission depletion microscopy (STED) and Förster resonance energy transfer (FRET) the molecular pathways through the SC, the main barrier of the skin, are determined for lipophilic and water-soluble molecules at a nanoscopic resolution. Read More

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http://dx.doi.org/10.1016/j.bbagen.2019.04.012DOI Listing

Light-induced formation of NO in endothelial cells by photoactivatable NADPH analogues targeting nitric-oxide synthase.

Biochim Biophys Acta Gen Subj 2019 Apr 12. Epub 2019 Apr 12.

LBPA, CNRS UMR8113, IDA FR3242, ENS Paris-Saclay, Université Paris-Saclay, F-94235 Cachan, France. Electronic address:

Background: Nitric-oxide synthases (NOS) catalyze the formation of NO using NADPH as electron donor. We have recently designed and synthesized a new series of two-photon absorbing and photoactivatable NADPH analogues (NT). These compounds bear one or two carboxymethyl group(s) on the 2'- or/and 3'-position(s) of the ribose in the adenosine moiety, instead of a 2'-phosphate group, and differ by the nature of the electron donor in their photoactivatable chromophore (replacing the nicotinamide moiety). Read More

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http://dx.doi.org/10.1016/j.bbagen.2019.04.004DOI Listing

The protein disulfide isomerase family: From proteostasis to pathogenesis.

Biochim Biophys Acta Gen Subj 2019 Apr 12. Epub 2019 Apr 12.

Frontier Research Institute for Interdisciplinary Sciences, Tohoku University, Aramaki aza Aoba 6-3, Aoba-ku, Sendai, Miyagi 980-8578, Japan. Electronic address:

In mammalian cells, nearly one-third of proteins are inserted into the endoplasmic reticulum (ER), where they undergo oxidative folding and chaperoning assisted by approximately 20 members of the protein disulfide isomerase family (PDIs). PDIs consist of multiple thioredoxin-like domains and recognize a wide variety of proteins via highly conserved interdomain flexibility. Although PDIs have been studied intensely for almost 50 years, exactly how they maintain protein homeostasis in the ER remains unknown, and is important not only for fundamental biological understanding but also for protein misfolding- and aggregation-related pathophysiology. Read More

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http://dx.doi.org/10.1016/j.bbagen.2019.04.003DOI Listing
April 2019
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An insight into the dependence of the deamination rate of human APOBEC3F on the length of single-stranded DNA, which is affected by the concentrations of APOBEC3F and single-stranded DNA.

Biochim Biophys Acta Gen Subj 2019 Apr 12. Epub 2019 Apr 12.

Institute of Advanced Energy, Kyoto University, Gokasho, Uji, Kyoto, Japan; Graduate School of Energy Science, Kyoto University, Gokasho, Uji, Kyoto, Japan. Electronic address:

Background: APOBEC3F (A3F), a member of the human APOBEC3 (A3) family of cytidine deaminases, acts as an anti-HIV-1 factor by deaminating deoxycytidine in the complementary DNA of the viral genome. A full understanding of the deamination behavior of A3F awaits further investigation.

Methods: The real-time NMR method and uracil-DNA glycosylase assay were used to track the activities of the C-terminal domain (CTD) of A3F at different concentrations of A3F-CTD and ssDNA. Read More

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http://dx.doi.org/10.1016/j.bbagen.2019.04.011DOI Listing

Antimicrobial peptide hybrid fluorescent protein based sensor array discriminate ten most frequent clinic isolates.

Biochim Biophys Acta Gen Subj 2019 Apr 12. Epub 2019 Apr 12.

Center for Clinical Laboratory Medicine of Zhongda Hospital, Southeast University, China. Electronic address:

Antimicrobial peptides killed bacteria through intercalating into the bacterial membrane. Their antimicrobial efficiencies varied in bacterial species and were affected by ion strength in the culture medium. A recombinant IGP protein consisted of an antimicrobial peptide, Ib-AMP4 fused with the Green Fluorescent Protein was expressed from E. Read More

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http://dx.doi.org/10.1016/j.bbagen.2019.04.010DOI Listing

Yeast Dop1 is required for glycosyltransferase retrieval from the trans-Golgi network.

Biochim Biophys Acta Gen Subj 2019 Apr 11. Epub 2019 Apr 11.

Key Laboratory of Carbohydrate Chemistry, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, Jiangsu 214122, China. Electronic address:

Background: Glycosyltransferases are type II membrane proteins that are responsible for glycan modification of proteins and lipids, and localize to distinct cisternae in the Golgi apparatus. During cisternal maturation, retrograde trafficking helps maintain the steady-state localization of these enzymes in the sub-compartments of the Golgi.

Methods: To understand how glycosyltransferases are recycled in the late Golgi complex, we searched for genes that are essential for budding yeast cell growth and that encode proteins localized in endosomes and in the Golgi. Read More

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http://dx.doi.org/10.1016/j.bbagen.2019.04.009DOI Listing

Metabolic activation triggered by cAMP in MCF-7 cells generates lethal vulnerability to combined oxamate/etomoxir.

Biochim Biophys Acta Gen Subj 2019 Apr 11. Epub 2019 Apr 11.

Department of Experimental, Diagnostic and Specialty Medicine (DIMES), University of Bologna, Italy. Electronic address:

Background: Altered energy metabolism is a biochemical fingerprint of cancer cells, widely recognized as one of the "hallmarks of cancer". Cancer cells show highly increased rates of glucose uptake and glycolysis, after which the resulting pyruvate is converted to lactate. The maintenance of this metabolic asset is warranted by lactate dehydrogenase A (LDH-A) and for this reason the development of novel LDH-targeted anticancer therapeutics is underway. Read More

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http://dx.doi.org/10.1016/j.bbagen.2019.04.008DOI Listing

Tripeptide IRW initiates differentiation in osteoblasts via the RUNX2 pathway.

Biochim Biophys Acta Gen Subj 2019 Apr 10. Epub 2019 Apr 10.

Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB, Canada. Electronic address:

Background: Osteoblasts maintain the structural integrity of bone via differentiation and mineralization; therefore, their malfunction or reduced activity can cause serious bone disorders. Although studies have demonstrated the association between nutrients and bone, research on food-derived bioactive peptides and bone health are scanty.

Methods: Osteoblasts MC3T3-E1 were treated with IRW (50 and 25 μM). Read More

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http://dx.doi.org/10.1016/j.bbagen.2019.04.007DOI Listing
April 2019
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Atomistic modeling and molecular dynamics analysis of human Ca1.2 channel using external electric field and ion pulling simulations.

Biochim Biophys Acta Gen Subj 2019 Apr 9;1863(6):1116-1126. Epub 2019 Apr 9.

Faculty Of Pharmacy And Pharmaceutical Sciences, University Of Alberta, Edmonton, Ab, Canada; Li Ka Shing Institute Of Virology, University Of Alberta, Edmonton, Alberta, Canada; Li Ka Shing Applied Virology Institute, University Of Alberta, Edmonton, Alberta, Canada. Electronic address:

Background: Human Ca1.2 (hCav1.2), a calcium selective voltage-gated channel, plays important roles in normal cardiac and neuronal functions. Read More

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http://dx.doi.org/10.1016/j.bbagen.2019.04.006DOI Listing
April 2019
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An SOD1 deficiency aggravates proteasome inhibitor bortezomib-induced testicular damage in mice.

Biochim Biophys Acta Gen Subj 2019 Apr 8;1863(6):1108-1115. Epub 2019 Apr 8.

Department of Biochemistry and Molecular Biology, Graduate School of Medical Science, Yamagata University, 2-2-2 Iidanishi, Yamagata 990-9585, Japan.

Proteasomes play a key role in maintaining cellular homeostasis by the proteolytic removal of proteins, including ubiquitinated proteins and/or oxidatively-damaged proteins. The proteasome inhibitor bortezomib (BTZ) has been reported to exert testicular toxicity in mice. In the current study, we treated SOD1-knockout (KO) mice with BTZ and investigated the issue of whether oxidative stress is involved in the development of testicular toxicity. Read More

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http://dx.doi.org/10.1016/j.bbagen.2019.04.005DOI Listing
April 2019
3 Reads

Sphingosine 1-phosphate receptor 1 regulates cell-surface localization of membrane proteins in endothelial cells.

Biochim Biophys Acta Gen Subj 2019 Apr 4;1863(6):1079-1087. Epub 2019 Apr 4.

The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325027, China; The Affiliated Kangning Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325007, China; School of Mental Health, Wenzhou Medical University, Wenzhou, Zhejiang 325035, China; The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325015, China. Electronic address:

The endothelial cell (EC) barrier disruption has been implicated in vascular leakage and pulmonary edema. Many reports have shown that the EC barrier dysfunction is regulated by the sphingosine-1-phophate (S1P)/S1P receptor-1 (S1PR1) axis. Identifying downstream effectors for the S1P/S1PR1 axis in pulmonary vasculature has been limited by mixed populations in vitro cultures that do not retain physiological EC phenotype and complex of tedious proteomics. Read More

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http://dx.doi.org/10.1016/j.bbagen.2019.04.001DOI Listing
April 2019
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Multicolor redox sensor proteins can visualize redox changes in various compartments of the living cell.

Biochim Biophys Acta Gen Subj 2019 Apr 4;1863(6):1098-1107. Epub 2019 Apr 4.

Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology, Nagatsuta-cho 4259, Midori-ku, Yokohama 226-8503, Japan; Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST), Chiyoda-Ku, Tokyo 102-0076, Japan. Electronic address:

Change in the intracellular redox state is a consequence of various metabolic reactions, which simultaneously regulates various physiological phenomena in cells. Monitoring the redox state in living cells is thus very important for understanding cellular physiology. Various genetically encoded fluorescent redox sensors have therefore been developed. Read More

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http://dx.doi.org/10.1016/j.bbagen.2019.01.016DOI Listing
April 2019
3 Reads

Allosteric transitions in hemoglobin revisited.

Authors:
Naoya Shibayama

Biochim Biophys Acta Gen Subj 2019 Apr 2. Epub 2019 Apr 2.

Division of Biophysics, Department of Physiology, Jichi Medical University, 3311-1 Yakushiji, Shimotsuke, Tochigi 329-0498, Japan. Electronic address:

Background: Human hemoglobin is an allosteric protein that exerts exquisite control over ligand binding through large-scale conformational changes. The two-state model without intermediates offers a simple qualitative description of the allosteric behavior of hemoglobin, as presented in textbooks. However, there is renewed interest in this topic due to recent experimental breakthroughs that show how hemoglobin actually undergoes conformational transitions in response to environmental changes. Read More

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http://dx.doi.org/10.1016/j.bbagen.2019.03.021DOI Listing

Kinetics and regioselectivity of three GH62 α-L-arabinofuranosidases from plant pathogenic fungi.

Biochim Biophys Acta Gen Subj 2019 Mar 30;1863(6):1070-1078. Epub 2019 Mar 30.

Department of Chemical Engineering and Applied Chemistry, University of Toronto, 200 College Street, Toronto, ON M5S 3E5, Canada; Departments of Animal Biosciences, University of Guelph, Guelph, ON N1G 2W1, Canada. Electronic address:

Backgound: Xylan is the second most abundant plant cell wall polysaccharide after cellulose with α-L-arabinofuranose (L-Araf) as one of the major side substituents. Capacity to degrade xylan is characteristic of many plant pathogens; and corresponding enzymes that debranch arabinoxylan provide tools to tailor xylan functionality or permit its full hydrolysis.

Method: Three GH62_2 family α-arabinofuranosidases (Abfs) from plant pathogenic fungi, NhaAbf62A from Nectria haematococca, SreAbf62A from Sporisorium reilianum and GzeAbf62A from Gibberella zeae, were recombinantly produced in Escherichia coli. Read More

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http://dx.doi.org/10.1016/j.bbagen.2019.03.020DOI Listing
March 2019
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Accelerating structural life science by paramagnetic lanthanide probe methods.

Biochim Biophys Acta Gen Subj 2019 Mar 28. Epub 2019 Mar 28.

Department of Chemistry, Faculty of Science, Hokkaido University, Sapporo, Hokkaido 060-0810, Japan; Graduate School of Chemical Sciences and Engineering, Hokkaido University, Sapporo, Hokkaido 060-8628, Japan.

We describe the recent progress of structural analysis methods exploiting paramagnetic lanthanide ions. In NMR spectroscopy, the paramagnetic effects induced by the trivalent lanthanide ions provide long-range (~40 Å) distance and angular information that can be exploited in protein structure determination, ligand screening, structure-based resonance assignment, and in-cell observation. The paramagnetic lanthanide ions can also be utilized in EPR spectroscopy, providing nanometer-scale distance measurement. Read More

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http://dx.doi.org/10.1016/j.bbagen.2019.03.018DOI Listing
March 2019
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Discovery of pyridoxal reductase activity as part of human vitamin B6 metabolism.

Biochim Biophys Acta Gen Subj 2019 Mar 27;1863(6):1088-1097. Epub 2019 Mar 27.

Department of Genetics, University Medical Center Utrecht, Utrecht, the Netherlands. Electronic address:

Background: Pyridoxal 5'-phosphate (PLP) is the active form of vitamin B6. Mammals cannot synthesize vitamin B6, so they rely on dietary uptake of the different B6 forms, and via the B6 salvage pathway they interconvert them into PLP. Humans possess three enzymes in this pathway: pyridoxal kinase, pyridox(am)ine phosphate oxidase and pyridoxal phosphatase. Read More

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http://dx.doi.org/10.1016/j.bbagen.2019.03.019DOI Listing
March 2019
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Characterization of acetyl-CoA synthetase kinetics and ATP-binding.

Biochim Biophys Acta Gen Subj 2019 Mar 28;1863(6):1040-1049. Epub 2019 Mar 28.

Department of Biochemistry and Molecular Biology and Immunology (B), Faculty of Chemistry, University of Murcia, Campus of Espinardo, Regional Campus of International Excellence "Campus Mare Nostrum", P.O. Box 4021, Murcia E-30100, Spain.

Background: The superfamily of adenylating enzymes is a large family of enzymes broadly distributed from bacteria to humans. Acetyl-CoA synthetase (Acs), member of this family, is a metabolic enzyme with an essential role in Escherichia coli (E. coli) acetate metabolism, whose catalytic activity is regulated by acetylation/deacetylation in vivo. Read More

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http://dx.doi.org/10.1016/j.bbagen.2019.03.017DOI Listing
March 2019
2 Reads

Microsystem for the single molecule analysis of membrane transport proteins.

Authors:
Rikiya Watanabe

Biochim Biophys Acta Gen Subj 2019 Mar 26. Epub 2019 Mar 26.

Molecular Physiology Laboratory, RIKEN, Saitama, Japan. Electronic address:

Micro-chamber arrays enable highly sensitive and quantitative bioassays at the single-molecule level. Accordingly, they are widely used for ultra-sensitive biomedical applications, e.g. Read More

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http://dx.doi.org/10.1016/j.bbagen.2019.03.016DOI Listing
March 2019
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The cell resealing technique for manipulating, visualizing, and elucidating molecular functions in living cells.

Biochim Biophys Acta Gen Subj 2019 Mar 23. Epub 2019 Mar 23.

Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 153-8902, Japan; Cell Biology Center, Institute of Innovative Research, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama, Kanagawa 226-8503, Japan; Laboratory of Frontier Image Analysis, Graduate School of Arts and Science, The University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 153-8902, Japan. Electronic address:

Background: Cell-based assays are essential for analyzing molecular functions and spatiotemporal information. The cell resealing technique, in which pore-forming toxins are used to permeabilize cell membranes, enables the delivery of various membrane-impermeable molecules inside cells.

Scope Of Review: We review the basics of the resealed cell system, including optimized protocols, assessment of cellular damage, and recovery following permeabilization of the membrane. Read More

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http://dx.doi.org/10.1016/j.bbagen.2019.03.015DOI Listing

O-GlcNAcylation disrupts STRA6-retinol signals in kidneys of diabetes.

Biochim Biophys Acta Gen Subj 2019 Mar 21;1863(6):1059-1069. Epub 2019 Mar 21.

Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan; Division of Endocrinology and Metabolism, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan; School of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan; Lipid Science and Aging Research Center, Kaohsiung Medical University, Kaohsiung, Taiwan; Institute of Medical Science and Technology, National Sun Yat-Sen University, Kaohsiung, Taiwan. Electronic address:

Background: O-GlcNAcylation is an important mechanism of diabetic complication. Retinoid homeostasis regulates cell-physiological functions through STRA6-retinol signaling. Therefore, we investigated whether O-GlcNAcylation disrupted STRA6-retinol signals in diabetes. Read More

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http://dx.doi.org/10.1016/j.bbagen.2019.03.014DOI Listing
March 2019
2 Reads

Structure and dynamics of Trichoderma harzianum Cel7B suggest molecular architecture adaptations required for a wide spectrum of activities on plant cell wall polysaccharides.

Biochim Biophys Acta Gen Subj 2019 Mar 19;1863(6):1015-1026. Epub 2019 Mar 19.

Instituto de Física de São Carlos, Universidade de São Paulo, Av. Trabalhador Sãocarlense, 400, São Carlos SP 13566-590, Brazil. Electronic address:

Cellulases from glycoside hydrolase family 7 (GH7) play crucial roles in plant lignocellulose deconstruction by fungi, but structural information available for GH7 fungal endoglucanases is limited when compared to the number of known sequences in the family. Here, we report the X-ray structure of the glycosylated catalytic domain (CD) of Trichoderma harzianum endoglucanase, ThCel7B, solved and refined at 2.9 Å resolution. Read More

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http://dx.doi.org/10.1016/j.bbagen.2019.03.013DOI Listing
March 2019
3 Reads

High-speed near-field fluorescence microscopy combined with high-speed atomic force microscopy for biological studies.

Biochim Biophys Acta Gen Subj 2019 Mar 16. Epub 2019 Mar 16.

Nano Life Science Institute (WPI-NanoLSI), Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan. Electronic address:

Background: High-speed atomic force microscopy (HS-AFM) has successfully visualized a variety of protein molecules during their functional activity. However, it cannot visualize small molecules interacting with proteins and even protein molecules when they are encapsulated. Thus, it has been desired to achieve techniques enabling simultaneous optical/AFM imaging at high spatiotemporal resolution with high correlation accuracy. Read More

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http://dx.doi.org/10.1016/j.bbagen.2019.03.011DOI Listing

Antimicrobial garlic-derived diallyl polysulfanes: Interactions with biological thiols in Bacillus subtilis.

Biochim Biophys Acta Gen Subj 2019 Mar 16;1863(6):1050-1058. Epub 2019 Mar 16.

School of Pharmacy, University of East Anglia, Norwich Research Park, Norwich NR4 7TJ, UK. Electronic address:

Background: Diallylpolysulfanes are the key constituents of garlic oils, known to exhibit broad spectrum anticancer and antimicrobial activity. Studies in vitro, and in mammalian cells, have shown they react, via thiol-polysulfane exchange, with their major low molecular weight thiol, glutathione. However, there are no detailed reports of diallylpolysulfane effects on other common thiol metabolites (cysteine and coenzyme A) or major thiol cofactors (e. Read More

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http://dx.doi.org/10.1016/j.bbagen.2019.03.012DOI Listing

Morphological and mechanical stability of bladder cancer cells in response to substrate rigidity.

Biochim Biophys Acta Gen Subj 2019 Mar 14;1863(6):1006-1014. Epub 2019 Mar 14.

Institute of Nuclear Physics, Polish Academy of Sciences, PL-31341 Kraków, Poland.

Background: Morphology of cells can be considered as an interplay between the accessibility of substrate anchoring sites, cytoskeleton properties and cellular deformability. To withstand tension induced by cell's environment, cells tend to spread out and, simultaneously, to remodel actin filament organization.

Methods: In this context, the use of polyacrylamide hydrogel substrates with a surface coated with laminin allows to trace remodeling of actin cytoskeleton during the interaction of cells with laminin-rich basement membrane. Read More

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http://dx.doi.org/10.1016/j.bbagen.2019.03.010DOI Listing

Investigation of quaternary structure of aggregating 3-ketosteroid dehydrogenase from Sterolibacterium denitrificans: In the pursuit of consensus of various biophysical techniques.

Biochim Biophys Acta Gen Subj 2019 Mar 12;1863(6):1027-1039. Epub 2019 Mar 12.

Jerzy Haber Institute of Catalysis and Surface Chemistry, Polish Academy of Sciences, Niezapominajek 8, PL30239, Krakow, Poland.

In this work we analyzed the quaternary structure of FAD-dependent 3-ketosteroid dehydrogenase (AcmB) from Sterolibacterium denitrificans, the protein that in solution forms massive aggregates (>600 kDa). Using size-excursion chromatography (SEC), dynamic light scattering (DLS), native-PAGE and atomic force microscopy (AFM) we studied the nature of enzyme aggregation. Partial protein de-aggregation was facilitated by the presence of non-ionic detergent such as Tween 20 or by a high degree of protein dilution but not by addition of a reducing agent or an increase of ionic strength. Read More

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http://dx.doi.org/10.1016/j.bbagen.2019.03.009DOI Listing
March 2019
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Corrigendum to "Combinatorial anticancer effects of curcumin and 5-fluorouracil loaded thiolated chitosan nanoparticles towards colon cancer treatment" [Biochimica et Biophysica Acta 1840 (2014) 2730-2743].

Biochim Biophys Acta Gen Subj 2019 May 12;1863(5):992. Epub 2019 Mar 12.

Amrita Centre for Nanosciences and Molecular Medicine, Amrita Institute of Medical Sciences and Research Centre, Amrita Vishwa Vidyapeetham University, Ponekkara, Kochi 682041, India.. Electronic address:

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http://dx.doi.org/10.1016/j.bbagen.2019.03.008DOI Listing
May 2019
8 Reads

Role and mechanism of homocysteine in affecting hepatic protein-tyrosine phosphatase 1B.

Biochim Biophys Acta Gen Subj 2019 May 7;1863(5):941-949. Epub 2019 Mar 7.

Medical School, University of Chinese Academy of Sciences, the Campus of Yanqi, Huai Rou, 101407 Beijing, China. Electronic address:

Background: Elevated homocysteine is epidemiologically related to insulin resistance. Protein-tyrosine phosphatase 1B (PTP1B) is a negative regulator of insulin signaling. However, the effect of homocysteine on PTP1B remains unclear. Read More

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http://dx.doi.org/10.1016/j.bbagen.2019.03.007DOI Listing

Understanding molecular features of aggregation-resistant tau conformer using oxidized monomer.

Biochim Biophys Acta Gen Subj 2019 Mar 8;1863(6):993-1005. Epub 2019 Mar 8.

Division of Molecular Medicine, Clinical Proteomics Unit, St. John's Research Institute, St. John's National Academy of Health Sciences, 100 Feet Road, Koramangala, Bangalore 560034, India. Electronic address:

Background: Aggregation of tau into paired helical filament (PHF) is a hallmark of Alzheimer's disease (AD), and Cys-mediated disulfide bond formation plays a vital role in tau fibrillation. While intermolecular disulfide bond between Cys residues in microtubule-binding repeat (MTBR) region facilitates tau aggregation, intramolecular disulfide bond attenuates the same, though the molecular basis for such phenomenon remains obscure. Thus intramolecular disulfide-bonded tau monomer might be an excellent model to understand the unique features of aggregation-resistant tau conformer. Read More

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http://dx.doi.org/10.1016/j.bbagen.2019.03.006DOI Listing
March 2019
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Number and brightness analysis to study spatio-temporal distribution of the angiotensin II AT and the endothelin-1 ET receptors: Influence of ligand binding.

Biochim Biophys Acta Gen Subj 2019 May 6;1863(5):917-924. Epub 2019 Mar 6.

Center of Innovation and Technology Transfer, Institute of Scientific Research and High Technology Services (INDICASAT AIP) Panama, Panama. Electronic address:

The angiotensin II AT and the endothelin 1 ET receptors play a crucial role in the pathogenesis of cardiovascular diseases like hypertension, heart failure, stroke, pulmonary hypertension, and cardiac hypertrophy. Both receptors are members of the rhodopsion-like superfamily of G protein-coupled receptors which can exist as monomers, dimers, and higher order aggregates. Recently, oligomerization of these two receptors have been described by several biophysical methods based mainly on luminescence and fluorescence energy transfer. Read More

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http://dx.doi.org/10.1016/j.bbagen.2019.03.004DOI Listing
May 2019
5 Reads

Lectinology 4.0: Altering modular (ga)lectin display for functional analysis and biomedical applications.

Biochim Biophys Acta Gen Subj 2019 May 6;1863(5):935-940. Epub 2019 Mar 6.

Institute of Physiological Chemistry, Faculty of Veterinary Medicine, Ludwig-Maximilians-University Munich, Veterinärstr. 13, 80539 Munich, Germany. Electronic address:

Background: Recognition of glycans by lectins is emerging as (patho)physiologically broadly used mode of cellular information transfer. Whereas the direct ligand-receptor contact is often already thoroughly characterized, the functional relevance of aspects of architecture such as modular design and valence of lectins is less well defined.

Scope Of Review: Following an introduction to modular lectin design, three levels of methodology are then reviewed that delineate lectin structure-activity relationships beyond glycan binding, with emphasis on domain shuffling. Read More

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http://dx.doi.org/10.1016/j.bbagen.2019.03.005DOI Listing
May 2019
7 Reads

A photo-cross-linking approach to monitor protein dynamics in living cells.

Biochim Biophys Acta Gen Subj 2019 Mar 6. Epub 2019 Mar 6.

Institute for Frontier Life and Medical Sciences, Kyoto University, Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan. Electronic address:

Background: Proteins, which comprise one of the major classes of biomolecules that constitute a cell, interact with other cellular factors during both their biogenesis and functional states. Studying not only static but also transient interactions of proteins is important to understand their physiological roles and regulation mechanisms. However, only a limited number of methods are available to analyze the dynamic behaviors of proteins at the molecular level in a living cell. Read More

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http://dx.doi.org/10.1016/j.bbagen.2019.03.003DOI Listing

Plant components can reduce methylmercury toxication: A mini-review.

Biochim Biophys Acta Gen Subj 2019 Mar 6. Epub 2019 Mar 6.

Department of Preventive Medicine and Public Health Laboratory Sciences, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu 212013, China; Center for Experimental Research, Affiliated Kunshan Hospital to Jiangsu University, Zhenjiang, Jiangsu 212013, China. Electronic address:

Background: Methylmercury (MeHg) is a ubiquitous environmental pollutant, with the nervous system as its main target; however, the neurotoxic mechanisms of MeHg have not been fully elucidated, and no effective therapeutic and preventive drugs are available to mitigate its toxicity. Recent evidence suggests a reduction in the toxicity of MeHg by natural plant extracts.

Scope Of Review: The aim of this review is to provide an overview of effective natural plant extracts and their putative biochemical mechanisms for blocking gut absorption, enhancing excretion and minimizing toxic effects of MeHg. Read More

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http://dx.doi.org/10.1016/j.bbagen.2019.01.012DOI Listing
March 2019
1 Read

Long chain lipid hydroperoxides increase the glutathione redox potential through glutathione peroxidase 4.

Biochim Biophys Acta Gen Subj 2019 May 5;1863(5):950-959. Epub 2019 Mar 5.

Environmental Public Health Division, National Health and Environmental Effects Research Laboratory, U.S. Environmental Protection Agency, Chapel Hill, NC, USA. Electronic address:

Background: Peroxidation of PUFAs by a variety of endogenous and xenobiotic electrophiles is a recognized pathophysiological process that can lead to adverse health effects. Although secondary products generated from peroxidized PUFAs have been relatively well studied, the role of primary lipid hydroperoxides in mediating early intracellular oxidative events is not well understood.

Methods: Live cell imaging was used to monitor changes in glutathione (GSH) oxidation in HAEC expressing the fluorogenic sensor roGFP during exposure to 9-hydroperoxy-10E,12Z-octadecadienoic acid (9-HpODE), a biologically important long chain lipid hydroperoxide, and its secondary product 9-hydroxy-10E,12Z-octadecadienoic acid (9-HODE). Read More

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http://dx.doi.org/10.1016/j.bbagen.2019.03.002DOI Listing

Serum protein N-glycosylation changes in multiple myeloma.

Biochim Biophys Acta Gen Subj 2019 May 5;1863(5):960-970. Epub 2019 Mar 5.

Center for Proteomics and Metabolomics, Leiden University Medical Center, Albinusdreef 2, 2333ZA Leiden, The Netherlands. Electronic address:

Background: Multiple myeloma is characterized by clonal proliferation of malignant plasma cells in the bone marrow that produce monoclonal immunoglobulins. N-glycosylation changes of these monoclonal immunoglobulins have been reported in multiple myeloma, but previous studies only detected limited serum N-glycan features.

Methods: Here, a more detailed study of the human serum N-glycome of 91 multiple myeloma patients and 51 controls was performed. Read More

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http://dx.doi.org/10.1016/j.bbagen.2019.03.001DOI Listing
May 2019
2 Reads

The characterisation of two members of the cytochrome P450 CYP150 family: CYP150A5 and CYP150A6 from Mycobacterium marinum.

Biochim Biophys Acta Gen Subj 2019 May 28;1863(5):925-934. Epub 2019 Feb 28.

Department of Chemistry, University of Adelaide, SA 5005, Australia. Electronic address:

Background: Actinobacteria, including the Mycobacteria, have a large component of cytochrome P450 family monooxygenases. This includes Mycobacterium tuberculosis, M. ulcerans and M. Read More

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http://dx.doi.org/10.1016/j.bbagen.2019.02.016DOI Listing
May 2019
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Induction of autophagy enhances apoptotic cell death via epidermal growth factor receptor inhibition by canertinib in cervical cancer cells.

Biochim Biophys Acta Gen Subj 2019 May 27;1863(5):903-916. Epub 2019 Feb 27.

Department of Clinical Biochemistry, Faculty of Medicine, Istinye University, Istanbul, Turkey. Electronic address:

Background: It has been known epidermal growth factor receptor (EGFR) frequently overexpressed in cervical cancer. High levels of EGFR expression in their tumors leads to a poor prognosis and inhibition frequently induces autophagy in cancer cells. This study aimed to investigate whether EGFR inhibition by canertinib induces autophagy and this induction influence the effect of Palladium (Pd) (II) complex and 5-fluorouracil (5-FU) especially in nontoxic doses. Read More

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http://dx.doi.org/10.1016/j.bbagen.2019.02.014DOI Listing

Direct visualization of avian influenza H5N1 hemagglutinin precursor and its conformational change by high-speed atomic force microscopy.

Biochim Biophys Acta Gen Subj 2019 Feb 27. Epub 2019 Feb 27.

WPI Nano Life Science Institute (WPI-NanoLSI), Kanazawa University, Kakuma-machi, Kanazawa, Japan.; Cell-Bionomics Research Unit, Innovative Integrated Bio-Research Core, Institute for Frontier Science Initiative, Kanazawa University, Kanazawa, Japan; Faculty of Natural System, Institute of Natural Science and Technology, Kanazawa University, Kanazawa, Japan. Electronic address:

Background: Hemagglutinin (HA) of influenza A is one of the key virulence factors that mediates the release of viral components in host cells. HA is initially synthesized as a trimeric precursor (HA0) and then it is cleaved by proteases to become a functional HA. Low pH induces irreversible conformational changes in both HA0 and HA but only HA is fusion compatible. Read More

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http://dx.doi.org/10.1016/j.bbagen.2019.02.015DOI Listing
February 2019
5 Reads

Monitoring Caco-2 to enterocyte-like cells differentiation by means of electric impedance analysis on printed sensors.

Biochim Biophys Acta Gen Subj 2019 May 25;1863(5):893-902. Epub 2019 Feb 25.

Department of Molecular and Translational Medicine, University of Brescia, Brescia, Italy.

Background: Colorectal adenocarcinoma cells (Caco-2) are a widely used model of intestinal barrier to study cancer development, toxicological assessments, absorption and metabolism in food science or drug discovery. Caco-2 spontaneously differentiate into a monolayer expressing several specific characteristics, typically showed by mature enterocytes. For in vitro experiments, it is crucial to identify non-invasive and non-destructive techniques able to evaluate the integrity and differentiation of the cells monolayer. Read More

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http://dx.doi.org/10.1016/j.bbagen.2019.02.008DOI Listing
May 2019
2 Reads

A health concern regarding the protein corona, aggregation and disaggregation.

Biochim Biophys Acta Gen Subj 2019 May 22;1863(5):971-991. Epub 2019 Feb 22.

Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran.

Nanoparticle (NP)-protein complexes exhibit the "correct identity" of NP in biological media. Therefore, protein-NP interactions should be closely explored to understand and modulate the nature of NPs in medical implementations. This review focuses mainly on the physicochemical parameters such as dimension, surface chemistry, morphology of NPs, and influence of pH on the formation of protein corona and conformational changes of adsorbed proteins by different kinds of techniques. Read More

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http://dx.doi.org/10.1016/j.bbagen.2019.02.012DOI Listing
May 2019
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4.381 Impact Factor

A novel membrane-disruptive antimicrobial peptide from frog skin secretion against cystic fibrosis isolates and evaluation of anti-MRSA effect using Galleria mellonella model.

Biochim Biophys Acta Gen Subj 2019 May 22;1863(5):849-856. Epub 2019 Feb 22.

Natural Drug Discovery Group, School of Pharmacy, Queen's University Belfast, Belfast, Northern Ireland BT9 7BL, UK.

Antimicrobial peptides from amphibian skin secretion are a promising source for the development of alternative antibiotics against the urgent antibiotic resistance. Methicillin-resistant S. aureus (MRSA) has been found to persist in both early and late disease course of cystic fibrosis (CF). Read More

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http://dx.doi.org/10.1016/j.bbagen.2019.02.013DOI Listing
May 2019
9 Reads

In Vitro interaction between yeast frataxin and superoxide dismutases: Influence of mitochondrial metals.

Biochim Biophys Acta Gen Subj 2019 May 21;1863(5):883-892. Epub 2019 Feb 21.

Interfaces, Traitements, Organisation et Dynamique des Systèmes (ITODYS), CNRS UMR 7086, Univ Paris Diderot, Sorbonne Paris Cité, 15 rue Jean-Antoine de Baïf, F-75205 Paris Cedex 13, France. Electronic address:

Background: Friedreich's ataxia results from a decreased expression of the nuclear gene encoding the mitochondrial protein, frataxin. Frataxin participates in the biosynthesis of iron-sulfur clusters and heme cofactors, as well as in iron storage and protection against oxidative stress. How frataxin interacts with the antioxidant defence components is poorly understood. Read More

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http://dx.doi.org/10.1016/j.bbagen.2019.02.011DOI Listing
May 2019
1 Read
4.381 Impact Factor

Polypeptide N-acetylgalactosaminyltransferase 18 non-catalytically regulates the ER homeostasis and O-glycosylation.

Biochim Biophys Acta Gen Subj 2019 May 21;1863(5):870-882. Epub 2019 Feb 21.

Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China; SCSB (China)-AIST (Japan) Joint Medical Glycomics Laboratory, Shanghai, China. Electronic address:

Mucin-type O-glycosylation plays important roles in various biological processes. It is initiated by a family of 20 conserved UDP-GalNAc: polypeptide N-acetylgalactosaminyltransferases (ppGalNAc-Ts). Unlike most ppGalNAc-Ts localized to the Golgi apparatus, ppGalNAc-T18 is predominantly distributed on the endoplasmic reticulum (ER) and exhibits no ppGalNAc-T catalytic activity in vitro. Read More

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http://dx.doi.org/10.1016/j.bbagen.2019.01.009DOI Listing
May 2019
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4.381 Impact Factor

Heparan sulfate in chronic kidney diseases: Exploring the role of 3-O-sulfation.

Biochim Biophys Acta Gen Subj 2019 May 19;1863(5):839-848. Epub 2019 Feb 19.

Institute of Cellular Medicine, Faculty of Medical Sciences, Newcastle University, NE2 4HH, UK. Electronic address:

One of the main feature of chronic kidney disease is the development of renal fibrosis. Heparan Sulfate (HS) is involved in disease development by modifying the function of growth factors and cytokines and creating chemokine gradients. In this context, we aimed to understand the function of HS sulfation in renal fibrosis. Read More

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http://dx.doi.org/10.1016/j.bbagen.2019.02.009DOI Listing

Iron-oxide nanoparticles target intracellular HSP90 to induce tumor radio-sensitization.

Biochim Biophys Acta Gen Subj 2019 May 20;1863(5):857-869. Epub 2019 Feb 20.

Radiation Biology & Health Sciences Division, Bhabha Atomic Research Centre, Mumbai 400085, India; Homi Bhabha National Institute, Anushakti Nagar, Mumbai 400085, India. Electronic address:

Background: Nanoparticle-based therapies have emerged as a promising approach to overcome limitations of conventional chemotherapy. Present study investigates the potential of oleic acid-functionalized iron-oxide nanoparticles (MN-OA) to enhance the radiation response of fibrosarcoma tumor and elucidates its underlying mechanism.

Methods: Various cellular and molecular assays (e. Read More

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http://dx.doi.org/10.1016/j.bbagen.2019.02.010DOI Listing
May 2019
4.381 Impact Factor

Intrinsic origin of amyloid aggregation: Behavior of histidine (εεε) and (δδδ) tautomer homodimers of Aβ (1-40).

Biochim Biophys Acta Gen Subj 2019 May 13;1863(5):795-801. Epub 2019 Feb 13.

Department of Chemistry, Sungkyunkwan University, Suwon 16419, South Korea; School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China. Electronic address:

Amyloid-beta protein (Aβ) accumulation in the brain, which is influenced by several factors, is a hallmark of Alzheimer's disease (AD). Despite the important role of histidine in stabilizing the fibrillar structure of the Aβ peptide at neutral pH, the effect of histidine tautomerism on Aβ peptide aggregation is still largely unknown. Histidine is in equilibrium between δ and ε tautomers and there are three histidine residues (H6, H13, and H14) in the Aβ(1-40) peptide. Read More

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http://dx.doi.org/10.1016/j.bbagen.2019.02.007DOI Listing
May 2019
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Involvement of caveolin-1 and CD36 in native LDL endocytosis by endothelial cells.

Biochim Biophys Acta Gen Subj 2019 May 12;1863(5):830-838. Epub 2019 Feb 12.

Institute of Chemistry and Biology of Membranes and Nano-objects (CBMN), UMR 5248, CNRS, University of Bordeaux, INP, F-33600 Pessac, France.

Atherosclerosis is a lipid disease characterized by accumulation of low density lipoprotein (LDL) in the artery wall. The transport of LDL across the endothelium of coronary artery is an initiating event of atherosclerosis, whose mechanism remains poorly understood. In the last decade, it has been shown that in caveolin-1 (Cav-1) deficient mice, LDL infiltration in aorta wall is decreased and CD36 expression in aortas is down-regulated, leading to regression of atherosclerotic lesions. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S03044165193000
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http://dx.doi.org/10.1016/j.bbagen.2019.01.005DOI Listing
May 2019
19 Reads

A multidimensional concept for mercury neuronal and sensory toxicity in fish - From toxicokinetics and biochemistry to morphometry and behavior.

Biochim Biophys Acta Gen Subj 2019 Feb 12. Epub 2019 Feb 12.

Department of Biology and CESAM, University of Aveiro, Aveiro 3810-193, Portugal. Electronic address:

Background: Neuronal and sensory toxicity of mercury (Hg) compounds has been largely investigated in humans/mammals with a focus on public health, while research in fish is less prolific and dispersed by different species. Well-established premises for mammals have been governing fish research, but some contradictory findings suggest that knowledge translation between these animal groups needs prudence [e.g. Read More

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http://dx.doi.org/10.1016/j.bbagen.2019.01.020DOI Listing
February 2019
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4.381 Impact Factor

Regulation of permeability transition pore opening in mitochondria by external NAD(H).

Biochim Biophys Acta Gen Subj 2019 May 11;1863(5):771-783. Epub 2019 Feb 11.

Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Institutskaya 3, Pushchino, Moscow Region 142290, Russia. Electronic address:

Background: The opening of the permeability transition pore (PTP) in mitochondria plays a critical role in the pathogenesis of numerous diseases. Mitochondrial matrix pyridine nucleotides are potent regulators of the PTP, but the role of extramitochondrial nucleotides is unclear.

Methods: The PTP opening was explored in isolated mitochondria and mitochondria in permeabilized differentiated and undifferentiated cells in the presence of added NAD(P)(H) in combination with Mg, adenine nucleotides (AN), and the inhibitors of AN translocase (ANT), voltage-dependent anion channel (VDAC), and cyclophilin D. Read More

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http://dx.doi.org/10.1016/j.bbagen.2019.01.003DOI Listing
May 2019
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AFM study: Cell cycle and probe geometry influences nanomechanical characterization of Panc1 cells.

Biochim Biophys Acta Gen Subj 2019 May 11;1863(5):802-812. Epub 2019 Feb 11.

Department of Biochemistry and Molecular Biology, Mayo Clinic, Jacksonville, FL, USA; Department of Pathology and Biomedical Engineering, Mayo Clinic, Jacksonville, FL, USA. Electronic address:

Atomic force microscope (AFM) is emerging as an immensely promising tool to study the cellular morphology with a nanometer scale resolution and to analyze nanomechanical properties (NPs) at various physiological conditions. Advancement of AFM technology enables studying living cells and differentiating cancer cell from normal cells based on topography and NPs. Though the trend overlaps from different literature; numerical values of nanomechanical readouts depict variations over a wide range. Read More

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http://dx.doi.org/10.1016/j.bbagen.2019.02.006DOI Listing

Human-induced pluripotent stems cells as a model to dissect the selective neurotoxicity of methylmercury.

Biochim Biophys Acta Gen Subj 2019 Feb 10. Epub 2019 Feb 10.

School of Health Sciences, Purdue University, West Lafayette, IN 47907-2051, United States. Electronic address:

Methylmercury (MeHg) is a potent neurotoxicant affecting both the developing and mature central nervous system (CNS) with apparent indiscriminate disruption of multiple homeostatic pathways. However, genetic and environmental modifiers contribute significant variability to neurotoxicity associated with human exposures. MeHg displays developmental stage and neural lineage selective neurotoxicity. Read More

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http://dx.doi.org/10.1016/j.bbagen.2019.02.002DOI Listing
February 2019
1 Read