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    PRDM14, a putative histone methyl-transferase, interacts with and decreases the stability and activity of the HOXA1 transcription factor.
    Biochim Biophys Acta 2018 Feb 19. Epub 2018 Feb 19.
    Animal Molecular and Cellular Biology Group, Institut des Sciences de la Vie (ISV), Université catholique de Louvain, Croix du Sud 5, 1348 Louvain-la-Neuve, Belgium. Electronic address:
    Understanding how the activity of transcription factors like HOX proteins is regulated remains a widely open question. In a recent screen for proteins interacting with HOXA1, we identified a PRDM protein family member, PRDM14, which is known to be transiently co-expressed with HOXA1 in epiblast cells before their specification towards somatic versus germ cell fate. Here, we confirm PRDM14 is an interactor of HOXA1 and we identify the homeodomain of HOXA1 as well as the PR domain and Zinc fingers of PRDM14 to be required for the interaction. Read More

    eNOS S-nitrosylation mediated OxLDL-induced endothelial dysfunction via increasing the interaction of eNOS with β-catenin.
    Biochim Biophys Acta 2018 Feb 19. Epub 2018 Feb 19.
    Key Laboratory of Cardiovascular and Cerebrovascular Medicine, Key Laboratory of Targeted Intervention of Cardiovascular Disease, Collaborative Innovation Center for Cardiovascular Disease Translational Medicine, Nanjing Medical University, Nanjing, China. Electronic address:
    Protein S-nitrosylation plays an important role in the progression of cardiovascular diseases. eNOS can be S-nitrosylated in endothelial cells, and this modification reversibly attenuates enzyme activity. Under physiological conditions, eNOS directly interacts with β-catenin. Read More

    Releasing iron from ferritin protein nanocage by reductive method: The role of electron transfer mediator.
    Biochim Biophys Acta 2018 Feb 19. Epub 2018 Feb 19.
    Department of Chemistry, National Institute of Technology, Rourkela 769008, Odisha, India. Electronic address:
    Background: Ferritin detoxifies excess of free Fe(II) and concentrates it in the form of ferrihydrite (FeO·xHO) mineral. When in need, ferritin iron is released for cellular metabolic activities. However, the low solubility of Fe(III) at neutral pH, its encapsulation by stable protein nanocage and presence of dissolved Olimits in vitro ferritin iron release. Read More

    RACK1/TRAF2 regulation of modulator of apoptosis (MOAP-1).
    Biochim Biophys Acta 2018 Feb 19. Epub 2018 Feb 19.
    Department of Pediatrics, Faculty of Science, National University of Singapore, Singapore 117543, Singapore; Oncology, Faculty of Medicine and Dentistry, 3055 Katz Group Centre for Pharmacy and Health Research, 113 Street 87 Avenue, University of Alberta, Edmonton, AB, Canada, T6G 2E1; Biochemistry, Faculty of Medicine and Dentistry, 3055 Katz Group Centre for Pharmacy and Health Research, 113 Street 87 Avenue, University of Alberta, Edmonton, AB, Canada, T6G 2E1; Member, Cancer Research Institute of Northern Alberta; Women and Children's Health Research Institute. Electronic address:
    MOAP-1 is a pro-apoptotic tumor suppressor molecule with a growing set of known interacting partners. We have demonstrated that during death receptor-dependent apoptosis, MOAP-1 is recruited to TNF-R1 or TRAIL-R1, followed by RASSF1A and Bax association. MOAP-1/Bax association promotes Bax conformational change resulting in the translocation of Bax into the mitochondrial membrane, mitochondrial membrane insertion and dysregulation resulting in several hallmark events that execute apoptosis. Read More

    Amputation of a C-terminal helix of the γ subunit increases ATP-hydrolysis activity of cyanobacterial FATP synthase.
    Biochim Biophys Acta 2018 Feb 19. Epub 2018 Feb 19.
    Laboratory for Chemistry and Life Science, Tokyo Institute of Technology, Nagatsuta 4259-R1-8, Midori-Ku, Yokohama 226-8503, Japan; Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST), Tokyo 102-0075, Japan. Electronic address:
    Fis a soluble part of FF-ATP synthase and performs a catalytic process of ATP hydrolysis and synthesis. The γ subunit, which is the rotary shaft of Fmotor, is composed of N-terminal and C-terminal helices domains, and a protruding Rossman-fold domain located between the two major helices parts. The N-terminal and C-terminal helices domains of γ assemble into an antiparallel coiled-coil structure, and are almost embedded into the stator ring composed of αβhexamer of the Fmolecule. Read More

    Small and large intestine express a truncated Dab1 isoform that assembles in cell-cell junctions and co-localizes with proteins involved in endocytosis.
    Biochim Biophys Acta 2018 Feb 19. Epub 2018 Feb 19.
    Departamento de Fisiología, Facultad de Farmacia, Universidad de Sevilla, Spain.
    Disabled-1 (Dab1) is an essential intracellular adaptor protein in the reelin pathway. Our previous studies in mice intestine showed that Dab1 transmits the reelin signal to cytosolic signalling pathways. Here, we determine the Dab1 isoform expressed in rodent small and large intestine, its subcellular location and co-localization with clathrin, caveolin-1 and N-Wasp. Read More

    Effect of ubiquinone-10 on the stability of biomimetic membranes of relevance for the inner mitochondrial membrane.
    Biochim Biophys Acta 2018 Feb 19. Epub 2018 Feb 19.
    Department of Chemistry-BMC, Uppsala University, Box 579, SE-75123 Uppsala, Sweden. Electronic address:
    Ubiquinone-10 (Q10) plays a pivotal role as electron-carrier in the mitochondrial respiratory chain, and is also well known for its powerful antioxidant properties. Recent findings suggest moreover that Q10 could have an important membrane stabilizing function. In line with this, we showed in a previous study that Q10 decreases the permeability to carboxyfluorescein (CF) and increases the mechanical strength of 1-palmitoyl-2-oleyl-sn-glycero-phosphocholine (POPC) membranes. Read More

    Low shear stress induces vascular eNOS uncoupling via autophagy-mediated eNOS phosphorylation.
    Biochim Biophys Acta 2018 Feb 18. Epub 2018 Feb 18.
    Department of Cardiology, Nanjing First Hospital, Nanjing Medical University, 210006 Nanjing, China. Electronic address:
    Uncoupled endothelial nitric oxide synthase (eNOS) produces Oinstead of nitric oxide (NO). Earlier, we reported rapamycin, an autophagy inducer and inhibitor of cellular proliferation, attenuated low shear stress (SS) induced Oproduction. Nevertheless, it is unclear whether autophagy plays a critical role in the regulation of eNOS uncoupling. Read More

    Iron transport kinetics through blood-brain barrier endothelial cells.
    Biochim Biophys Acta 2018 Feb 18. Epub 2018 Feb 18.
    School of Mechanical and Materials Engineering, Washington State University, Pullman, WA 99164-2920, United States. Electronic address:
    Background: Transferrin and its receptors play an important role during the uptake and transcytosis of iron through blood-brain barrier (BBB) endothelial cells (ECs) to maintain iron homeostasis in BBB endothelium and brain. Any disruptions in the cell environment may change the distribution of transferrin receptors on the cell surface, which eventually alter the homeostasis and initiate neurodegenerative disorders. In this paper, we developed a comprehensive mathematical model that considers the necessary kinetics for holo-transferrin internalization and acidification, apo-transferrin recycling, and exocytosis of free iron and transferrin-bound iron through basolateral side of BBB ECs. Read More

    Interaction between amyloidogenic proteins and biomembranes in protein misfolding diseases: Mechanisms, contributors, and therapy.
    Biochim Biophys Acta 2018 Feb 18. Epub 2018 Feb 18.
    Tongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan, China, 430030. Electronic address:
    The toxic deposition of misfolded amyloidogenic proteins is associated with more than fifty protein misfolding diseases (PMDs), including Alzheimer's disease, Parkinson's disease and type 2 diabetes mellitus. Protein deposition is a multi-step process modulated by a variety of factors, in particular by membrane-protein interaction. The interaction results in permeabilization of biomembranes contributing to the cytotoxicity that leads to PMDs. Read More

    Unveiling network-based functional features through integration of gene expression into protein networks.
    Biochim Biophys Acta 2018 Feb 18. Epub 2018 Feb 18.
    Department of Systems Biology and Bioinformatics, University of Rostock, 18051 Rostock, Germany. Electronic address:
    Decoding health and disease phenotypes is one of the fundamental objectives in biomedicine. Whereas high-throughput omics approaches are available, it is evident that any single omics approach might not be adequate to capture the complexity of phenotypes. Therefore, integrated multi-omics approaches have been used to unravel genotype-phenotype relationships such as global regulatory mechanisms and complex metabolic networks in different eukaryotic organisms. Read More

    ERK1/2/mTOR/Stat3 pathway-mediated autophagy alleviates traumatic brain injury-induced acute lung injury.
    Biochim Biophys Acta 2018 Feb 18. Epub 2018 Feb 18.
    Department of Neurosurgery, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, China. Electronic address:
    Acute lung injury (ALI) is one of several complications in patients with traumatic brain injury (TBI). Autophagy is a primary homeostatic process that promotes cell survival under stress. Accumulating evidence implicates autophagy in the pathogenesis of ALI under various conditions. Read More

    Cdk-related kinase 9 regulates RNA polymerase II mediated transcription in Toxoplasma gondii.
    Biochim Biophys Acta 2018 Feb 18. Epub 2018 Feb 18.
    National Institute of Animal Biotechnology, Hyderabad, India.
    Cyclin-dependent kinases are an essential part of eukaryotic transcriptional machinery. In Apicomplexan parasites, the role and relevance of the kinases in the multistep process of transcription seeks more attention given the absence of full repertoire of canonical Cdks and cognate cyclin partners. In this study, we functionally characterize T. Read More

    UHRF1 regulates CDH1 via promoter associated non-coding RNAs in prostate cancer cells.
    Biochim Biophys Acta 2018 Feb 18. Epub 2018 Feb 18.
    Department of Biotechnology and Life Sciences, University of Insubria, 21052 Busto Arsizio, VA, Italy. Electronic address:
    Non-coding RNAs (ncRNAs) transcribed from the promoter and the downstream region can affect the expression of the corresponding coding genes. It has been shown that sense-directed ncRNAs arising from the promoter region of the E-cadherin gene (CDH1) mediate its repression. Here, we show that an antisense-directed ncRNA (paRCDH1-AS) transcribed from the CDH1 promoter is necessary for its expression. Read More

    A novel neuropilin-1-binding sequence in the human T-cell lymphotropic virus type 1 envelope glycoprotein.
    Biochim Biophys Acta 2018 Feb 16. Epub 2018 Feb 16.
    Department of Safety Research on Blood and Biological Products, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama, Tokyo 208-0011, Japan. Electronic address:
    Entry of human T-cell lymphotropic virus type 1 (HTLV-1) into host cells is mainly mediated by interactions between the viral envelope glycoprotein surface unit (SU) and three host receptors: glucose transporter type 1, heparin/heparan sulfate proteoglycan, and neuropilin-1 (Nrp1). Here, we analyzed the interaction between HTLV-1 SU and Nrp1 using nuclear magnetic resonance and isothermal titration calorimetry. We found that two SU peptides, residues 85-94 and residues 304-312, bound directly to the Nrp1 b1 domain with affinities of 7. Read More

    Reciprocal regulation of the Cadherin-11/Stat3 axis by caveolin-1 in mouse fibroblasts and lung carcinoma cells.
    Biochim Biophys Acta 2018 Feb 16. Epub 2018 Feb 16.
    Department of Biomedical and Molecular Sciences, Pathology and Molecular Medicine, and Queen's University Cancer Research Institute, Queen's University, Kingston, Ontario, Canada K7L 3N6.
    Caveolin-1 (Cav1) is an integral plasma membrane protein and a complex regulator of signal transduction. The Signal Transducer and Activator of Transcription-3 (Stat3) is activated by a number of receptor and non-receptor tyrosine kinases and is positively implicated in cancer. Despite extensive efforts, the relationship between Cav1 and Stat3 has been a matter of controversy. Read More

    Evidence for a mitochondrial ATP-regulated potassium channel in human dermal fibroblasts.
    Biochim Biophys Acta 2018 Feb 16. Epub 2018 Feb 16.
    Laboratory of Intracellular Ion Channels, Nencki Institute of Experimental Biology, Warsaw, Poland.
    Mitochondrial ATP-regulated potassium channels are present in the inner membrane of the mitochondria of various cells. In the present study, we show for the first time mitochondrial ATP-regulated potassium channels in human dermal fibroblast cells. Using the patch-clamp technique on the inner mitochondrial membrane of fibroblasts, we detected a potassium channel with a mean conductance equal to 100 pS in symmetric 150 mM KCl. Read More

    In vitro studies of disease-linked variants of human tRNA nucleotidyltransferase reveal decreased thermal stability and altered catalytic activity.
    Biochim Biophys Acta 2018 Feb 15. Epub 2018 Feb 15.
    Department of Chemistry and Biochemistry and Centre for Structural and Functional Genomics, Concordia University, 7141 Sherbrooke St. W., Montréal H4B 1R6, Québec, Canada. Electronic address:
    Mutations in the human TRNT1 gene encoding tRNA nucleotidyltransferase (tRNA-NT), an essential enzyme responsible for addition of the CCA (cytidine-cytidine-adenosine) sequence to the 3'-termini of tRNAs, have been linked to disease phenotypes including congenital sideroblastic anemia with B-cell immunodeficiency, periodic fevers and developmental delay (SIFD) or retinitis pigmentosa with erythrocyte microcytosis. The effects of these disease-linked mutations on the structure and function of tRNA-NT have not been explored. Here we use biochemical and biophysical approaches to study how five SIFD-linked amino acid substitutions (T154I, M158V, L166S, R190I and I223T), residing in the N-terminal head and neck domains of the enzyme, affect the structure and activity of human tRNA-NT in vitro. Read More

    A novel β-glucosidase isolated from the microbial metagenome of Lake Poraquê (Amazon, Brazil).
    Biochim Biophys Acta 2018 Feb 15. Epub 2018 Feb 15.
    Laboratory of Molecular Biology, Department of Genetics and Evolution, Federal University of São Carlos, SP, Brazil. Electronic address:
    The Amazon region holds most of the biological richness of Brazil. Despite their ecological and biotechnological importance, studies related to microorganisms from this region are limited. Metagenomics leads to exciting discoveries, mainly regarding non-cultivable microorganisms. Read More

    Optical Fiber Tips for Biological Applications: from Light Confinement, Biosensing to Bioparticles Manipulation.
    Biochim Biophys Acta 2018 Feb 15. Epub 2018 Feb 15.
    INESC Technology and Science, Portugal; Faculty of Engineering, University of Porto, Portugal.
    Background: The tip of an optical fiber has been considered an attractive platform in Biology. The simple cleaved end of an optical fiber can be machined, patterned and/or functionalized, acquiring unique properties enabling the exploitation of novel optical phenomena. Prompted by the constant need to measure and manipulate nanoparticles, the invention of the Scanning Near-Field Optical Microscopy (SNOM) triggered the optimization and development of novel fiber tip microfabrication methods. Read More

    The biological basis of treating jaw discrepancies: An interplay of mechanical forces and skeletal configuration.
    Biochim Biophys Acta 2018 Feb 14. Epub 2018 Feb 14.
    Department of Biological Chemistry, Cellular and Molecular Biomechanics Unit, Medical School, National and Kapodistrian University of Athens, 11527 Athens, Greece. Electronic address:
    Jaw discrepancies and malrelations affect a large proportion of the general population and their treatment is of utmost significance for individuals' health and quality of life. The aim of their therapy is the modification of aberrant jaw development mainly by targeting the growth potential of the mandibular condyle through its cartilage, and the architectural shape of alveolar bone through a suture type of structure, the periodontal ligament. This targeted treatment is achieved via external mechanical force application by using a wide variety of intraoral and extraoral appliances. Read More

    The G protein-coupled P2Yreceptor promotes colorectal cancer tumorigenesis by inhibiting apoptosis.
    Biochim Biophys Acta 2018 Feb 14. Epub 2018 Feb 14.
    Department of Anatomy and Cell Biology, Faculty of Medicine and Health Sciences, Université de Sherbrooke, Sherbrooke, Quebec, Canada. Electronic address:
    Colorectal tumors are immersed in an array of tumor-promoting factors including extracellular nucleotides such as uridine 5'‑diphosphate (UDP). UDP is the endogenous agonist of the G protein-coupled P2Yreceptor (P2YR), which may contribute to the formation of a tumor-promoting microenvironment by coordinating resistance to apoptosis. Colorectal cancer (CRC) was chemically induced in P2ry6 knockout (P2ry6) mice using azoxymethane and dextran sulfate sodium challenges. Read More

    Nutraceuticals as therapeutic agents for atherosclerosis.
    Biochim Biophys Acta 2018 Feb 14. Epub 2018 Feb 14.
    Cardiff School of Biosciences, Cardiff University, Sir Martin Evans Building, Museum Avenue, Cardiff CF10 3AX, UK. Electronic address:
    Atherosclerosis, a chronic inflammatory disorder of medium and large arteries and an underlying cause of cardiovascular disease (CVD), is responsible for a third of all global deaths. Current treatments for CVD, such as optimized statin therapy, are associated with considerable residual risk and several side effects in some patients. The outcome of research on the identification of alternative pharmaceutical agents for the treatment of CVD has been relatively disappointing with many promising leads failing at the clinical level. Read More

    The accumulation of assembly intermediates of the mitochondrial complex I matrix arm is reduced by limiting glucose uptake in a neuronal-like model of MELAS syndrome.
    Biochim Biophys Acta 2018 Feb 14. Epub 2018 Feb 14.
    UMR CNRS 6015-INSERM U1083, MitoVasc Institute, Angers University, Angers, France; Biochemistry and Genetics Department, Angers Hospital, F-49000, France. Electronic address:
    Ketogenic diet (KD) which combined carbohydrate restriction and the addition of ketone bodies has emerged as an alternative metabolic intervention used as an anticonvulsant therapy or to treat different types of neurological or mitochondrial disorders including MELAS syndrome. MELAS syndrome is a severe mitochondrial disease mainly due to the m.3243A > G mitochondrial DNA mutation. Read More

    Expression and therapeutic implications of cyclin-dependent kinase 4 (CDK4) in osteosarcoma.
    Biochim Biophys Acta 2018 Feb 13. Epub 2018 Feb 13.
    Department of Pharmacy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, People's Republic of China; Sarcoma Biology Laboratory, Center for Sarcoma and Connective Tissue Oncology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA. Electronic address:
    Overexpression and/or hyperactivation of cyclin-dependent kinase 4 (CDK4) has been found in many types of human cancers, and a CDK4 specific inhibitor, palbociclib, has been recently approved by the FDA for the treatment of breast cancer. However, the expression and the therapeutic potential of CDK4 in osteosarcoma remain unclear. In the present study, CDK4 was found to be highly expressed in human osteosarcoma tissues and cell lines as compared with normal human osteoblasts. Read More

    Cardiac and placental mitochondrial characterization in a rabbit model of intrauterine growth restriction.
    Biochim Biophys Acta 2018 Feb 13. Epub 2018 Feb 13.
    Muscle Research and Mitochondrial Function Laboratory, Cellex - IDIBAPS, Faculty of Medicine and Health Science, University of Barcelona, Internal Medicine Service, Hospital Clínic of Barcelona, Barcelona, Spain; CIBERER, U722, Madrid, Spain. Electronic address:
    Background: Intrauterine growth restriction (IUGR) is associated with cardiovascular remodeling persisting into adulthood. Mitochondrial bioenergetics, essential for embryonic development and cardiovascular function, are regulated by nuclear effectors as sirtuins. A rabbit model of IUGR and cardiovascular remodeling was generated, in which heart mitochondrial alterations were observed by microscopic and transcriptomic analysis. Read More

    Improved strength of silk fibers in Bombyx mori trimolters induced by an anti-juvenile hormone compound.
    Biochim Biophys Acta 2018 Feb 13;1862(5):1148-1156. Epub 2018 Feb 13.
    State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China. Electronic address:
    Background: Bombyx mori silk fibers with thin diameters have advantages of lightness and crease-resistance. Many studies have used anti-juvenile hormones to induce trimolters in order to generate thin silk; however, there has been comparatively little analysis of the morphology, structure and mechanical properties of trimolter silk.

    Methods: This study induced two kinds of trimolters by appling topically anti-juvenile hormones and obtained thin diameter silk. Read More

    Corrigendum to "energy landscape of the intact and destabilized FMO antennas from C. tepidum and the L122Q mutant: Low temperature spectroscopy and modeling study" [Biochim. Biophys. Acta Bioenerg. 1859 (2018) 165-173].
    Biochim Biophys Acta 2018 Feb 12. Epub 2018 Feb 12.
    Department of Chemistry, Kansas State University, Manhattan, KS 66506, USA; Department of Physics, Kansas State University, Manhattan, KS 66506, USA. Electronic address:

    Insertion of dengue E into lipid bilayers studied by neutron reflectivity and molecular dynamics simulations.
    Biochim Biophys Acta 2018 Feb 12. Epub 2018 Feb 12.
    Sandia National Laboratories, Albuquerque, NM, United States. Electronic address:
    The envelope (E) protein of Dengue virus rearranges to a trimeric hairpin to mediate fusion of the viral and target membranes, which is essential for infectivity. Insertion of E into the target membrane serves to anchor E and possibly also to disrupt local order within the membrane. Both aspects are likely to be affected by the depth of insertion, orientation of the trimer with respect to the membrane normal, and the interactions that form between trimer and membrane. Read More

    Helix formation and stability in membranes.
    Biochim Biophys Acta 2018 Feb 12. Epub 2018 Feb 12.
    Department of Chemistry and Biochemistry, University of Arkansas, Fayetteville, AR, 72701, USA. Electronic address:
    In this article we review current understanding of basic principles for the folding of membrane proteins, focusing on the more abundant alpha-helical class. Membrane proteins, vital to many biological functions and implicated in numerous diseases, fold into their active conformations in the complex environment of the cell bilayer membrane. While many membrane proteins rely on the translocon and chaperone proteins to fold correctly, others can achieve their functional form in the absence of any translation apparatus or other aides. Read More

    Phosphatidylserine-stimulated production of N-acyl-phosphatidylethanolamines by Ca-dependent N-acyltransferase.
    Biochim Biophys Acta 2018 Feb 12. Epub 2018 Feb 12.
    Department of Biochemistry, Kagawa University School of Medicine, 1750-1 Ikenobe, Miki, Kagawa 761-0793, Japan. Electronic address:
    N-Acyl-phosphatidylethanolamine (NAPE) is known to be a precursor for various bioactive N-acylethanolamines including the endocannabinoid anandamide. NAPE is produced in mammals through the transfer of an acyl chain from certain glycerophospholipids to phosphatidylethanolamine (PE) by Ca-dependent or -independent N-acyltransferases. The Ɛ isoform of mouse cytosolic phospholipase A(cPLAƐ) was recently identified as a Ca-dependent N-acyltransferase (Ca-NAT). Read More

    PI3K/AKT/mTOR activation and autophagy inhibition plays a key role in increased cholesterol during IL-17A mediated inflammatory response in psoriasis.
    Biochim Biophys Acta 2018 Feb 9. Epub 2018 Feb 9.
    Functional Genomics Unit, CSIR-Institute of Genomics and Integrative Biology, Mall Road, Delhi -110007, India; Academy of Scientific & Innovative Research, CSIR-Institute of Genomics and Integrative Biology, Delhi 110007, India. Electronic address:
    Psoriasis is an immune-mediated inflammatory disease of the skin. Previous studies including ours have shown that IL-17A plays a major role in its pathogenesis; however, its precise molecular mechanism of action is not well understood. Cytokines like TNF α and IL-23 are also important in mediating the disease and some studies have also reported autophagy as a novel mechanism by which cytokines controls the immune response. Read More

    4-PBA ameliorates cellular homeostasis in fibroblasts from osteogenesis imperfecta patients by enhancing autophagy and stimulating protein secretion.
    Biochim Biophys Acta 2018 Feb 9. Epub 2018 Feb 9.
    aDepartment of Molecular Medicine, Biochemistry Unit, University of Pavia, Pavia, 27100, Italy. Electronic address:
    The clinical phenotype in osteogenesis imperfecta (OI) is attributed to the dominant negative function of mutant type I collagen molecules in the extracellular matrix, by altering its structure and function. Intracellular retention of mutant collagen has also been reported, but its effect on cellular homeostasis is less characterized. Using OI patient fibroblasts carrying mutations in the α1(I) and α2(I) chains we demonstrate that retained collagen molecules are responsible for endoplasmic reticulum (ER) enlargement and activation of the unfolded protein response (UPR) mainly through the eukaryotic translation initiation factor 2 alpha kinase 3 (PERK) branch. Read More

    Probing the extended lipid anchorage with cytochrome c and liposomes containing Diacylphosphatidylglycerol lipids.
    Biochim Biophys Acta 2018 Feb 9. Epub 2018 Feb 9.
    Department of Chemistry and Biochemistry, Providence College, Providence, RI 02918, United States. Electronic address:
    Experiments investigating the adsorption and desorption of cytochrome c onto and from liposomes containing 50 mol% 1,2-diacylphosphatidylglycerol lipids [10:0, 12:0, 14:0, 16:0, 18:1(Δ9 cis)] with 1,2-dioleoyl-sn-glycero-3-phosphatidylcholine (DOPC) in pH 7.4 buffered solutions of low to moderate ionic strength are reported. Fluorescence experiments show that cytochrome c has a similar adsorption affinity for the five labeled 50 mol% PG liposome systems investigated. Read More

    Corrigendum to "LMD proteomics provides evidence for hippocampus field-specific motor protein abundance changes with relevance to Alzheimer's disease" [BBAPAP 1865 (6) (2017) 703-714].
    Biochim Biophys Acta 2018 Feb 8. Epub 2018 Feb 8.
    Leibniz-Institut für Analytische Wissenschaften -ISAS- e. V., Dortmund, Germany.; Medizinische Fakultät, Medizinische Proteom-Center (MPC), Ruhr-Universität Bochum, 44801 Bochum, Germany.

    Tyr120Asp mutation alters domain flexibility and dynamics of MeCP2 DNA binding domain leading to impaired DNA interaction: Atomistic characterization of a Rett syndrome causing mutation.
    Biochim Biophys Acta 2018 Feb 8. Epub 2018 Feb 8.
    Department of Informatics, Institute of Computational Science, Università della Svizzera Italiana, Lugano, Switzerland. Electronic address:
    Mutations in the X-linked MECP2 gene represent the main origin of Rett syndrome, causing a profound intellectual disability in females. MeCP2 is an epigenetic transcriptional regulator containing two main functional domains: a methyl-CpG binding domain (MBD) and a transcription repression domain (TRD). Over 600 pathogenic mutations were reported to affect the whole protein; almost half of missense mutations affect the MBD. Read More

    Protein 4.1N is required for the formation of the lateral membrane domain in human bronchial epithelial cells.
    Biochim Biophys Acta 2018 Feb 8;1860(5):1143-1151. Epub 2018 Feb 8.
    School of Life Sciences, Zhengzhou University, Zhengzhou 450001, China. Electronic address:
    The membrane skeleton forms a scaffold on the cytoplasmic side of the plasma membrane. The erythrocyte membrane represents an archetype of such structural organization. It has been documented that a similar membrane skeleton also exits in the Golgi complex. Read More

    Insulin-eukaryotic model membrane interaction: Mechanistic insight of insulin fibrillation and membrane disruption.
    Biochim Biophys Acta 2018 Feb 9. Epub 2018 Feb 9.
    Department of Biophysics, P-1/12 CIT Scheme VII (M), Kolkata 700054, India. Electronic address:
    Injection of exogenous insulin in the subcutaneous mass has been a proven therapy for type II diabetes. However, chronic administration of insulin often develops local amyloidosis at the injection site, pathologically known as "Insulin Ball". This reduces the insulin bioavailability and exacerbates the disease pathology. Read More

    Electrostatic and hydrophobic interactions of lipid-associated α-synuclein: The role of a water-limited interfaces in amyloid fibrillation.
    Biochim Biophys Acta 2018 Feb 8. Epub 2018 Feb 8.
    Department of Chemistry, Korea University, Seoul 02841, Republic of Korea. Electronic address:
    Human α‑synuclein (αSyn) is an intrinsically disordered protein (IDP) whose biological and pathological functions in brain neuronal cells have not yet been fully elucidated. αSyn intrinsically participates in aiding neurotransmitter trafficking through αSyn the association with lipid membranes. However, lipid-associated states of αSyn also induce amyloid self-assembly that is linked to the pathogenesis of various synucleinopathies. Read More

    Atomistic-level study of the interactions between hIAPP protofibrils and membranes: Influence of pH and lipid composition.
    Biochim Biophys Acta 2018 Feb 9. Epub 2018 Feb 9.
    Basic Science Program, Leidos Biomedical Research, Inc., Cancer and Inflammation Program, National Cancer Institute, Frederick, MD 21702, United States; Department of Human Genetics and Molecular Medicine, Sackler School of Medicine, Sackler Institute of Molecular Medicine, Tel Aviv University, Tel Aviv 69978, Israel. Electronic address:
    The pathology of type 2 diabetes mellitus is associated with the aggregation of human islet amyloid polypeptide (hIAPP) and aggregation-mediated membrane disruption. The interactions of hIAPP aggregates with lipid membrane, as well as the effects of pH and lipid composition at the atomic level, remain elusive. Herein, using molecular dynamics simulations, we investigate the interactions of hIAPP protofibrillar oligomers with lipids, and the membrane perturbation that they induce, when they are partially inserted in an anionic dipalmitoyl-phosphatidylglycerol (DPPG) membrane or a mixed dipalmitoyl-phosphatidylcholine (DPPC)/DPPG (7:3) lipid bilayer under acidic/neutral pH conditions. Read More

    Interactions between RNAP III transcription machinery and tRNA processing factors.
    Biochim Biophys Acta 2018 Feb 9. Epub 2018 Feb 9.
    Molecular Genetics Laboratory, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi 110067, India. Electronic address:
    Eukaryotes have at least three nuclear RNA polymerases to carry out transcription. While RNA polymerases I and II are responsible for ribosomal RNA transcription and messenger RNA transcription, respectively, RNA Polymerase III transcribes approximately up to 300 nt long noncoding RNAs, including tRNA. For all three RNAPs, the nascent transcripts generated undergo extensive post-transcriptional processing. Read More

    2,4-Di-tert-butylphenol, a potential HDAC6 inhibitor, induces senescence and mitotic catastrophe in human gastric adenocarcinoma AGS cells.
    Biochim Biophys Acta 2018 Feb 7. Epub 2018 Feb 7.
    Subtropical/Tropical Organism Gene Bank, Jeju National University, Jeju 63243, Republic of Korea; Faculty of Biotechnology, College of Applied Life Sciences, Jeju National University, SARI, Jeju 63243, Republic of Korea. Electronic address:
    The natural product 2,4-di-tert-butylphenol (DTBP) has a wide spectrum of biological functions, including anticancer activities, although the underlying mechanisms are poorly understood. Here, we found that DTBP induces senescence in human gastric adenocarcinoma AGS cells as evidenced by upregulation of p21 and Rb and increased β-galactosidase activity. DTBP also induces mitotic catastrophe and generates multinucleated cells, which is accompanied by an increase in the proportion of polymerized tubulin, possibly caused by inhibition of HDAC6 enzyme activity. Read More

    Ablation of C/EBP homologous protein attenuates renal fibrosis after ureteral obstruction by reducing autophagy and microtubule disruption.
    Biochim Biophys Acta 2018 Feb 6. Epub 2018 Feb 6.
    Department of Anatomy and BK21 Plus, Kyungpook National University School of Medicine, 680 Gukchaebosang-ro, Junggu, Daegu 41944, Republic of Korea. Electronic address:
    Fibrosis is an undesirable consequence of injury and a critical problem in many diseases. Recent studies have demonstrated an association of C/EBP homologous protein (CHOP) with fibrosis. We investigated the mechanism of CHOP in kidney fibrosis progression after unilateral ureteral obstruction (UUO) using Chop gene-deleted (Chop) mice and their wild-type littermates (Chop). Read More

    Inhibition of Echis carinatus venom by DNA, a promising therapeutic molecule for snakebite management.
    Biochim Biophys Acta 2018 Feb 7;1862(5):1115-1125. Epub 2018 Feb 7.
    DOS in Biochemistry, University of Mysore, Manasagangotri, Mysuru 570 006, India. Electronic address:
    Background: E. carinatus bite is a serious threat to South-Asian countries including India, as it causes the highest number of deaths and debilitating sustained tissue necrosis at the bite site. One of our previous studies has demonstrated the strong interaction between DNA and E. Read More

    Hyperactivated m-calpain affects acquisition of doxorubicin resistance in breast cancer cells.
    Biochim Biophys Acta 2018 Feb 7;1862(5):1126-1133. Epub 2018 Feb 7.
    College of Pharmacy, Graduate School of Pharmaceutical Sciences, Ewha Womans University, Seoul, 03760, Republic of Korea. Electronic address:
    Background: Doxorubicin is commonly using chemotherapeutic agents for breast cancer. However, doxorubicin has limitations in clinical use because of dose-dependent cardiotoxicity and drug resistance. Despite of previously reported studies about mechanisms of doxorubicin resistance including overexpression of P-gp and abnormal expression and mutation of topoisomerase IIα, resistance to this agent still abundantly occur and is regarded as a major obstacle to successful treatment. Read More

    Structural stability of purified human CFTR is systematically improved by mutations in nucleotide binding domain 1.
    Biochim Biophys Acta 2018 Feb 6. Epub 2018 Feb 6.
    Department of Cell Biology and Biochemistry, and Center for Membrane Protein Research, Texas Tech University Health Sciences Center, 3601 4th Street, Stop 6540, Lubbock, TX 79430, USA. Electronic address:
    The Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) is an ABC transporter containing two transmembrane domains forming a chloride ion channel, and two nucleotide binding domains (NBD1 and NBD2). CFTR has presented a formidable challenge to obtain monodisperse, biophysically stable protein. Here we report a comprehensive study comparing effects of single and multiple NBD1 mutations on stability of both the NBD1 domain alone and on purified full length human CFTR. Read More

    Molecular simulation aspects of amyloid peptides at membrane interface.
    Biochim Biophys Acta 2018 Feb 6. Epub 2018 Feb 6.
    Department of Chemical and Biomolecular Engineering, The University of Akron, Akron, OH 44325, United States. Electronic address:
    The interactions of amyloid peptides with cell membranes play an important role in maintaining the integrity and functionality of cell membrane. A thorough molecular-level understanding of the structure, dynamics, and interactions between amyloid peptides and cell membranes is critical to amyloid aggregation and toxicity mechanisms for the bench-to-bedside applications. Here we review the most recent computational studies of amyloid peptides at model cell membranes. Read More

    Nontypeable Haemophilus influenzae DNA stimulates type I interferon expression via STING signaling pathway.
    Biochim Biophys Acta 2018 Feb 5;1865(4):665-673. Epub 2018 Feb 5.
    Key Laboratory of Diagnostic Medicine Designated by the Ministry of Education, Chongqing Medical University, 400016 Chongqing, China; School of Laboratory Medicine, Chongqing Medical University, 400016 Chongqing, China. Electronic address:
    Nontypeable Haemophilus influenzae (NTHI) is one of the leading causes of acute exacerbations of COPD (AECOPD). Although the immunoregulation function of NTHI outer member protein and endotoxin were confirmed, the role of NTHI DNA in activating immune responses remains to be elucidated. In this study, we found expression of IFN-β and IFN stimulated gene CXCL10 in host cells was forcefully elevated after treating with NTHI and NTHI DNA. Read More

    HMGB1-mediated DNA bending: Distinct roles in increasing p53 binding to DNA and the transactivation of p53-responsive gene promoters.
    Biochim Biophys Acta 2018 Feb 6;1861(3):200-210. Epub 2018 Feb 6.
    Laboratory of Analysis of Chromosomal Proteins, Institute of Biophysics of the Czech Academy of Sciences, Brno, Czech Republic.
    HMGB1 is a chromatin-associated protein that has been implicated in many important biological processes such as transcription, recombination, DNA repair, and genome stability. These functions include the enhancement of binding of a number of transcription factors, including the tumor suppressor protein p53, to their specific DNA-binding sites. HMGB1 is composed of two highly conserved HMG boxes, linked to an intrinsically disordered acidic C-terminal tail. Read More

    Long noncoding RNA complementarity and target transcripts abundance.
    Biochim Biophys Acta 2018 Feb 6;1861(3):224-234. Epub 2018 Feb 6.
    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, SC 29425, USA; Laboratory of Genetics, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224, USA. Electronic address:
    Eukaryotic mRNA metabolism regulates its stability, localization, and translation using complementarity with counter-part RNAs. To modulate their stability, small and long noncoding RNAs can establish complementarity with their target mRNAs. Although complementarity of small interfering RNAs and microRNAs with target mRNAs has been studied thoroughly, partial complementarity of long noncoding RNAs (lncRNAs) with their target mRNAs has not been investigated clearly. Read More

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