101,208 results match your criteria Biochimica et Biophysica Acta[Journal]


Depletion of Ubiquilin induces an augmentation in soluble ubiquitinated Drosophila TDP-43 to drive neurotoxicity in the fly.

Biochim Biophys Acta 2018 Jun 21. Epub 2018 Jun 21.

Department of Applied Biology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan; The Center for Advanced Insect Research, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan. Electronic address:

The proteostasis machinery has critical functions in metabolically active cells such as neurons. Ubiquilins (UBQLNs) may decide the fate of proteins, with its ability to bind and deliver ubiquitinated misfolded or no longer functionally required proteins to the ubiquitin-proteasome system (UPS) and/or autophagy. Missense mutations in UBQLN2 have been linked to X-linked dominant amyotrophic lateral sclerosis with frontotemporal dementia (ALS-FTD). Read More

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mTORC1 pathway in DNA damage response.

Biochim Biophys Acta 2018 Jun 21. Epub 2018 Jun 21.

CNRS UMR 8126, Université Paris-Sud 11, Institut Gustave Roussy, 114, rue Edouard Vaillant, 94805 Villejuif, France. Electronic address:

Living organisms have evolved various mechanisms to control their metabolism and response to various stresses, allowing them to survive and grow in different environments. In eukaryotes, the highly conserved mechanistic target of rapamycin (mTOR) signaling pathway integrates both intracellular and extracellular signals and serves as a central regulator of cellular metabolism, proliferation and survival. A growing body of evidence indicates that mTOR signaling is closely related to another cellular protection mechanism, the DNA damage response (DDR). Read More

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Residue-specific mobility changes in soluble oligomers of the prion protein define regions involved in aggregation.

Biochim Biophys Acta 2018 Jun 21. Epub 2018 Jun 21.

Department of Biochemistry, University of Alberta, Edmonton T6G 2H7, Canada.

Prion (PrP) diseases are neurodegenerative diseases characterized by the formation of β-sheet rich, insoluble and protease resistant protein deposits (called PrP) that occur throughout the brain. Formation of synthetic or in vitro PrP can occur through on-pathway toxic oligomers. Similarly, toxic and infectious oligomers identified in cell and animal models of prion disease indicate that soluble oligomers are likely intermediates in the formation of insoluble PrP. Read More

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The role of PNPLA3 in health and disease.

Authors:
P Pingitore S Romeo

Biochim Biophys Acta 2018 Jun 20. Epub 2018 Jun 20.

Department of Molecular and Clinical Medicine, University of Gothenburg, Sweden; Cardiology Department, Sahlgrenska University Hospital, Gothenburg, Sweden; Clinical Nutrition Unit, Department of Medical and Surgical Sciences, Magna Graecia University, Catanzaro, Italy. Electronic address:

The human patatin-like phospholipase domain-containing 3 (PNPLA3) gene encodes for a protein of 481 amino-acids. The variant rs738409 is a cytosine to guanine substitution, encoding for the isoleucine to methionine substitution at position 148 (I148M) of the protein. This variant is strongly associated with the entire spectrum of liver disease. Read More

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Genetic re-engineering of polyunsaturated phospholipid profile of Saccharomyces cerevisiae identifies a novel role for Cld1 in mitigating the effects of cardiolipin peroxidation.

Biochim Biophys Acta 2018 Jun 20. Epub 2018 Jun 20.

Department of Biological Sciences, Wayne State University, Detroit, MI, United States. Electronic address:

Cardiolipin (CL) is a unique phospholipid localized almost exclusively within the mitochondrial membranes where it is synthesized. Newly synthesized CL undergoes acyl remodeling to produce CL species enriched with unsaturated acyl groups. Cld1 is the only identified CL specific phospholipase in yeast and is required to initiate the CL remodeling pathway. Read More

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June 2018
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MicroRNAs of miR-17-92 cluster increase gene expression by targeting mRNA-destabilization pathways.

Biochim Biophys Acta 2018 Jun 20. Epub 2018 Jun 20.

Department of Biomedical Sciences, College of Medicine, Korea University, Seoul 02841, Republic of Korea. Electronic address:

MicroRNAs (miRNAs) of the miR-17-92 cluster are overexpressed in human cancers, and their enforced expression is tumorigenic in mouse models. A number of genes are reported to be targets of these miRNAs and are implicated in their tumorigenic potential. However, the mode of action by miRNAs suggests that global analysis of their targets is required to understand their cellular roles. Read More

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Heterodimerization of MaTCP proteins modulates the transcription of MaXTH10/11 genes during banana fruit ripening.

Biochim Biophys Acta 2018 Jun 20. Epub 2018 Jun 20.

State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources/Guangdong Key Laboratory for Postharvest Science, College of Horticultural Science, South China Agricultural University, Guangzhou 510642, PR China. Electronic address:

The biological processes involved in banana fruit ripening are extremely complex and modulated by a number of genes such as transcription factors (TFs). Although TFs like MADS, ERF and NAC are implicated in controlling banana ripening, little is known about other TFs such as TCP in this process. In this work, 25 MaTCPs named MaTCP1 to MaTCP25 were characterized from our previously reported transcriptomes related to banana ripening. Read More

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Tunable cell-surface mimetics as engineered cell substrates.

Biochim Biophys Acta 2018 Jun 20. Epub 2018 Jun 20.

Department of Chemistry and Chemical Biology, Indiana University-Purdue University, Indianapolis 46202, USA; Integrated Nanosystems Development Institute, IUPUI, Indianapolis, IN, USA. Electronic address:

Most recent breakthroughs in understanding cell adhesion, cell migration, and cellular mechanosensitivity have been made possible by the development of engineered cell substrates of well-defined surface properties. Traditionally, these substrates mimic the extracellular matrix (ECM) environment by the use of ligand-functionalized polymeric gels of adjustable stiffness. However, such ECM mimetics are limited in their ability to replicate the rich dynamics found at cell-cell contacts. Read More

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June 2018
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Cardiolipin synthases of Escherichia coli have phospholipid class specific phospholipase D activity dependent on endogenous and foreign phospholipids.

Biochim Biophys Acta 2018 Jun 19. Epub 2018 Jun 19.

Department of Biochemistry and Cell Biology, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 2, 3584CM Utrecht, the Netherlands. Electronic address:

E. coli has three Cls-isoenzymes for cardiolipin (CL) synthesis but the differences between these three enzymes remain unresolved. All three Cls enzymes contain the phospholipase D (PLD) characteristic HKD motive and synthesize CL using PLD activity. Read More

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The intracellular domain of the leptin receptor prevents mitochondrial depolarization and mitophagy.

Biochim Biophys Acta 2018 Jun 19. Epub 2018 Jun 19.

Cytokine Receptor Laboratory, Faculty of Medicine and Health Sciences, Department of Biochemistry, Ghent University, Ghent, Belgium; Center for Medical Biotechnology, VIB, Ghent, Belgium.. Electronic address:

Hypothalamic leptin receptor (LR) signaling regulates body weight by balancing food intake and energy expenditure. It is well established that the human LR undergoes ectodomain shedding, but little is known about the fate of the remaining cytosolic domain. This study demonstrates that regulated intramembrane proteolysis (RIP) releases the LR intracellular domain (LR ICD), which translocates to the mitochondria where it binds to SOCS6. Read More

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NLRX1 resides in mitochondrial RNA granules and regulates mitochondrial RNA processing and bioenergetic adaptation.

Biochim Biophys Acta 2018 Jun 19. Epub 2018 Jun 19.

Department of Biochemistry, Faculty of Science, The M.S. University of Baroda, Vadodara, 390002, Gujarat, India. Electronic address:

The role of mitochondria is emerging in regulation of innate immunity, inflammation and cell death beyond its primary role in energy metabolism. Mitochondria act as molecular platform for immune adaptor protein complexes, which participate in innate immune signaling. The mitochondrial localized immune adaptors are widely expressed in non-immune cells, however their role in regulation of mitochondrial function and metabolic adaption is not well understood. Read More

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Inhibition of the AnxA1/FPR1 autocrine axis reduces MDA-MB-231 breast cancer cell growth and aggressiveness in vitro and in vivo.

Biochim Biophys Acta 2018 Jun 19. Epub 2018 Jun 19.

Laboratory of Nanobiotechnology, Institute of Genetics and Biochemistry, Federal University of Uberlândia, Uberlândia, MG, Brazil. Electronic address:

Breast Cancer (BC) is a highly heterogeneous disease whose most aggressive behavior is displayed by triple-negative breast cancer (TNBC), which lacks an efficient targeted therapy. Despite its controversial role, one of the proteins that having been linked with BC is Annexin A1 (AnxA1), which is a Ca binding protein that acts modulating the immune system, cell membrane organization and vesicular trafficking. In this work we analyzed tissue microarrays of BC samples and observed a higher expression of AnxA1 in TNBCs and in lymph node metastasis. Read More

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Hepatic Rac1 GTPase contributes to liver-mediated basal immune homeostasis and LPS-induced endotoxemia.

Biochim Biophys Acta 2018 Jun 19. Epub 2018 Jun 19.

Institute of Toxicology, Medical Faculty, Heinrich Heine University Duesseldorf, Germany. Electronic address:

Background: The Ras-homologous GTPase Rac1 plays a key role in the regulation of gene expression, cytoskeleton-associated processes and cell death as well as carcinogenesis and inflammation. Here, we investigated the impact of Rac1 signaling on liver-mediated immune homeostasis.

Methods: We employed a constitutive Alb-Cre driven rac1 knock-out and a poly I:C inducible Mx1-Cre based knock-out model and analyzed cytokine expression profiles in liver and other organs under basal situation and following LPS-induced endotoxemia by flow cytometry, qRT-PCR and immunocytochemistry. Read More

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June 2018
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Photoinduction of electron transport on the acceptor side of PSI in Synechocystis PCC 6803 mutant deficient in flavodiiron proteins Flv1 and Flv3.

Biochim Biophys Acta 2018 Jun 19. Epub 2018 Jun 19.

Department of Genetics, Faculty of Biology, Moscow State University, 119991 Moscow, Russia.

After transferring the dark-acclimated cyanobacteria to light, flavodiiron proteins Flv1/Flv3 serve as a main electron acceptor for PSI within the first seconds because Calvin cycle enzymes are inactive in the dark. Synechocystis PCC 6803 mutant Δflv1/Δflv3 devoid of Flv1 and Flv3 retained the PSI chlorophyll P700 in the reduced state over 10 s ((Helman et al., 2003); Allahverdiyeva et al. Read More

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The regulatory subunit ε in Escherichia coli FF-ATP synthase.

Biochim Biophys Acta 2018 Jun 19. Epub 2018 Jun 19.

Single-Molecule Microscopy Group, Jena University Hospital, Friedrich Schiller University, Jena, Germany. Electronic address:

F-type ATP synthases are extraordinary multisubunit proteins that operate as nanomotors. The Escherichia coli (E. coli) enzyme uses the proton motive force (pmf) across the bacterial plasma membrane to drive rotation of the central rotor subunits within a stator subunit complex. Read More

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June 2018
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Development and validation of an improved diced electrophoresis gel assay cutter-plate system for enzymomics studies.

Biochim Biophys Acta 2018 Jun 18. Epub 2018 Jun 18.

Drug Discovery Initiative, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.

Diced electrophoresis gel (DEG) assay is a methodology to identify enzymes with a specified activity in complex cell or tissue lysates by means of two-dimensional separation using isoelectric focusing and native PAGE, followed by dicing of the gel into small pieces that are assayed separately, and digestion and peptide fingerprinting to identify the protein(s) of interest in positive wells. The existing hand-made system has some disadvantages, and here we describe the development and validation of an improved cutter-plate system that enables simple, reliable and reproducible DEG assay in a 384-well plate-based format with signal readout using fluorometric or LC-MS-based reaction monitoring. To illustrate the usefulness of this system, we describe its application to profile esterase activities in ovarian adenocarcinoma SKOV3 cell lysate and mouse liver lysate that activate a fluorogenic substrate, fluorescein dibutyrate (FDBu), as well as esterase activities in mouse liver lysate that activate S-bromobenzylglutathione dicyclopentyl ester (BBGDC), a prodrug of anti-tumor agent S-bromobenzylglutathione. Read More

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The MELAS mutation m.3243A>G promotes reactivation of fetal cardiac genes and an epithelial-mesenchymal transition-like program via dysregulation of miRNAs.

Biochim Biophys Acta 2018 Jun 18. Epub 2018 Jun 18.

RNA Modification and Mitochondrial Diseases Laboratory, Centro de Investigación Príncipe Felipe (CIPF), Carrer d'Eduardo Primo Yúfera 3, Valencia 46012, Spain; Centro de Investigación Biomédica en Red de Enfermedades Raras (CIBERER) node 721, Madrid 28029, Spain. Electronic address:

The pathomechanisms underlying oxidative phosphorylation (OXPHOS) diseases are not well-understood, but they involve maladaptive changes in mitochondria-nucleus communication. Many studies on the mitochondria-nucleus cross-talk triggered by mitochondrial dysfunction have focused on the role played by regulatory proteins, while the participation of miRNAs remains poorly explored. MELAS (mitochondrial encephalomyopathy, lactic acidosis, and stroke-like episodes) is mostly caused by mutation m. Read More

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The emerging role of circRNAs and their clinical significance in human cancers.

Biochim Biophys Acta 2018 Jun 18. Epub 2018 Jun 18.

Department of Integrative Oncology, Fudan University Shanghai Cancer Center, Shanghai 200032, China; Department of Oncology, Shanghai Medical College, Fudan University, Shanghai 2000332, China. Electronic address:

Circular RNA (circRNA), a recently discovered subclass of non-coding RNAs (ncRNAs), forms a covalently closed loop with neither a 5' cap structure nor a 3' polyadenylated tail. Generated from precursor mRNA (pre-mRNA) through "backsplicing" (a type of alternative RNA splicing), the majority of circRNAs are located in the cytoplasm and are widespread among living organisms. They are stable and conserved and exhibit spatiotemporal-specific expression. Read More

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Joker: An algorithm to insert patterns into sequences for designing antimicrobial peptides antimicrobial peptides.

Biochim Biophys Acta 2018 Jun 18. Epub 2018 Jun 18.

S-Inova Biotech, Pós-graduação em Biotecnologia, Universidade Católica Dom Bosco, Campo Grande, MS, Brazil; Centro de Análises Proteômicas e Bioquímicas. Programa de Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, Brasília, DF, Brazil; Programa de Pós-graduação em Patologia Molecular, Universidade de Brasília, Brasília, Brazil. Electronic address:

The rise of bacterial resistance makes it necessary to find innovative alternatives to control bacterial infections. Antimicrobial peptides (AMPs) have been considered as the new generation of antimicrobial agents. Based on the fact that AMPs are sequence-dependent, a linguistic model for designing AMPs was previously developed. Read More

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The psychoactive substance of cannabis Δ9-tetrahydrocannabinol (THC) negatively regulates CFTR in airway cells.

Biochim Biophys Acta 2018 Jun 18. Epub 2018 Jun 18.

Department of Veterinary Biosciences, The Ohio State University, Columbus, OH, USA; Davis Heart and Lung Research Institute, The Ohio State University, Columbus, OH, USA. Electronic address:

Background: Marijuana consumption is on the rise in the US but the health benefits of cannabis smoking are controversial and the impact of cannabis components on lung homeostasis is not well-understood. Lung function requires a fine regulation of the ion channel CFTR, which is responsible for fluid homeostasis and mucocilliary clearance. The goal of this study was to assess the effect that exposure to Δ9-tetrahydrocannabinol (THC), the psychoactive substance present in marijuana, has on CFTR expression and function. Read More

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June 2018
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Correlated fluorescence quenching and topographic mapping of Light-Harvesting Complex II within surface-assembled aggregates and lipid bilayers.

Biochim Biophys Acta 2018 Jun 18. Epub 2018 Jun 18.

Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield S10 2TN, UK.

Light-Harvesting Complex II (LHCII) is a chlorophyll-protein antenna complex that efficiently absorbs solar energy and transfers electronic excited states to photosystems I and II. Under excess light intensity LHCII can adopt a photoprotective state in which excitation energy is safely dissipated as heat, a process known as Non-Photochemical Quenching (NPQ). In vivo NPQ is triggered by combinatorial factors including transmembrane ΔpH, PsbS protein and LHCII-bound zeaxanthin, leading to dramatically shortened LHCII fluorescence lifetimes. Read More

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The long periodicity phase (LPP) controversy part I: The influence of a natural-like ratio of the CER[EOS] analogue [EOS]-br in a CER[NP]/[AP] based stratum corneum modelling system: A neutron diffraction study.

Biochim Biophys Acta 2018 Jun 17. Epub 2018 Jun 17.

Institute of Applied Dermatopharmacy at the Martin Luther University Halle-Wittenberg (IADP), Weinbergweg 23, 06120 Halle/Saale, Germany; Institute of Pharmacy, Martin Luther University Halle-Wittenberg (MLU), Wolfgang-Langenbeck-Str. 4, 06120 Halle/Saale, Germany. Electronic address:

This study used neutron diffraction to investigate a ceramide-[NP] C24/[AP] C24 /[EOS]-br C30/cholesterol/lignoceric acid (0.6: 0.3: 0. Read More

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LncRNA TDRG1 enhances tumorigenicity in endometrial carcinoma by binding and targeting VEGF-A protein.

Biochim Biophys Acta 2018 Jun 18. Epub 2018 Jun 18.

Department of Obstetrics and Gynecology, The Third Affiliated Hospital of Guangzhou Medical University, Key laboratory for Major Obstetric Diseases of Guangdong Province, and Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institute in Guangdong Province, Guangzhou 510150, PR China. Electronic address:

Endometrial carcinoma is one of the most frequently diagnosed cancers in females. Long non-coding RNAs (lncRNAs) have been associated with cancer; its role in endometrial carcinoma is an emerging area of research. In this article, lncRNA TDRG1 expression in human endometrial carcinoma tissues and normal endometrial tissues was quantified by qRT-PCR. Read More

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Effect of bedaquiline on the functions of rat liver mitochondria.

Biochim Biophys Acta 2018 Jun 16. Epub 2018 Jun 16.

Mari State University, pl. Lenina 1, Yoshkar-Ola, Mari El 424001, Russia.

The paper considers the effects of bedaquiline (BDQ), an antituberculous preparation of the new generation, on rat liver mitochondria. It was shown that 50 μM BDQ inhibited mitochondrial respiration measured with substrates of complexes I and II (glutamate/malate and succinate/rotenone systems respectively) in the states V and V. At the same time, at concentrations below 50 μM, BDQ slightly stimulated respiration with substrates of complex I in the state V. Read More

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Effects of GM1 on brain spectrin-aminophospholipid interactions.

Biochim Biophys Acta 2018 Jun 16. Epub 2018 Jun 16.

Crystallography & Molecular Biology Division, Saha Institute of Nuclear Physics, 1/AF, Bidhannagar, Kolkata 700064, India; Homi Bhabha National Institute, Mumbai 400094, India. Electronic address:

Spectrin, a major component of the membrane skeletal meshwork of metazoan cells, is implicated to associate with membrane domains and is known to act as a scaffold for stabilization and activation of different signalling modules. We have studied the effect of GM1 (monosialotetrahexosyl ganglioside), a well-known model ganglioside and a signalling moiety, on the interaction of non-erythroid brain spectrin with both saturated and unsaturated aminophospholipids by spectroscopic methods. We observe that GM1 modulates brain spectrin-aminophospholipid interaction to the greatest degree whereas its effect on erythroid spectrin is not as pronounced. Read More

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June 2018
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Sensitivity of peripheral membrane proteins to the membrane context: A case study of phosphatidylserine and the TIM proteins.

Biochim Biophys Acta 2018 Jun 16. Epub 2018 Jun 16.

Program in Biophysical Sciences, Institute for Biophysical Dynamics, The University of Chicago, Chicago, IL, United States of America; Department of Chemistry, The University of Chicago, Chicago, IL, United States of America; James Franck Institute, The University of Chicago, Chicago, IL, United States of America. Electronic address:

There is a diverse class of peripheral membrane-binding proteins that specifically bind phosphatidylserine (PS), a lipid that signals apoptosis or cell fusion depending on the membrane context of its presentation. PS-receptors are specialized for particular PS-presenting pathways, indicating that they might be sensitive to the membrane context. In this review, we describe a combination of thermodynamic, structural, and computational techniques that can be used to investigate the mechanisms underlying this sensitivity. Read More

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June 2018
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Mitochondrial therapy promotes regeneration of injured hippocampal neurons.

Biochim Biophys Acta 2018 Jun 15. Epub 2018 Jun 15.

Institute of Molecular Medicine, National Tsing Hua University, Hsinchu 30013, Taiwan, ROC; Department of Medical Science, National Tsing Hua University, Hsinchu 30013, Taiwan, ROC. Electronic address:

Due to the inhibitory microenvironment and reduced intrinsic growth capacity of neurons, neuronal regeneration of central nervous system remains challenging. Neurons are highly energy demanding and require sufficient mitochondria to support cellular activities. In response to stimuli, mitochondria undergo fusion/fission cycles to adapt to environment. Read More

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Prazosin induced lysosomal tubulation interferes with cytokinesis and the endocytic sorting of the tumour antigen CD98hc.

Biochim Biophys Acta 2018 Jun 15;1865(9):1211-1229. Epub 2018 Jun 15.

Research Unit Functional Proteomics and Metabolic Pathways, Institute of Pathology, Medical University of Graz, Auenbruggerplatz 25, 8036 Graz, Austria; Omics Center Graz, BioTechMed-Graz, Stiftingtalstrasse 24, 8010 Graz, Austria.

The quinazoline based drug prazosin (PRZ) is a potent inducer of apoptosis in human cancer cells. We recently reported that PRZ enters cells via endocytosis and induces tubulation of the endolysosomal system. In a proteomics approach aimed at identifying potential membrane proteins with binding affinity to quinazolines, we detected the oncoprotein CD98hc. Read More

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Myocardin-related transcription factor A (MRTF-A) contributes to acute kidney injury by regulating macrophage ROS production.

Biochim Biophys Acta 2018 Jun 14. Epub 2018 Jun 14.

Key Laboratory of Targeted Intervention of Cardiovascular Disease and Collaborative Innovation Center for Cardiovascular Translational Medicine, Department of Pathophysiology, Nanjing Medical University, Nanjing, China. Electronic address:

A host of pathogenic factors induce acute kidney injury (AKI) leading to insufficiencies of renal function. In the present study we evaluated the role of myocardin-related transcription factor A (MRTF-A) in the pathogenesis of AKI. We report that systemic deletion of MRTF-A or inhibition of MRTF-A activity with CCG-1423 significantly attenuated AKI in mice induced by either ischemia-reperfusion or LPS injection. Read More

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Expression of AGPAT2, an enzyme involved in the glycerophospholipid/triacylglycerol biosynthesis pathway, is directly regulated by HIF-1 and promotes survival and etoposide resistance of cancer cells under hypoxia.

Biochim Biophys Acta 2018 Jun 14. Epub 2018 Jun 14.

Laboratory of Physiology, Faculty of Medicine, University of Thessaly, Larissa, Greece. Electronic address:

Hypoxia inducible factor-1 (HIF-1) supports survival of normal cells under low oxygen concentration and cancer cells in the hypoxic tumor microenvironment. This involves metabolic reprogramming via upregulation of glycolysis, downregulation of oxidative phosphorylation and, less well documented, effects on lipid metabolism. To investigate the latter, we examined expression of relevant enzymes in cancer cells grown under hypoxia. Read More

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Insights into mechanism and functional consequences of heme binding to hemolysin-activating lysine acyltransferase HlyC from Escherichia coli.

Biochim Biophys Acta 2018 Jun 14. Epub 2018 Jun 14.

Pharmaceutical Biochemistry and Bioanalytics, Pharmaceutical Institute, University of Bonn, 53121 Bonn, Germany. Electronic address:

Background: Tight regulation of heme homeostasis is a critical mechanism in pathogenic bacteria since heme functions as iron source and prosthetic group, but is also toxic at elevated concentrations. Hemolysin-activating lysine-acyltransferase (HlyC) from Escherichia coli is crucial for maturation of hemolysin A, which lyses several mammalian cells including erythrocytes liberating large amounts of heme for bacterial uptake. A possible impact and functional consequences of the released heme on events employing bacterial HlyC have remained unexplored. Read More

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June 2018
2 Reads

Structural basis of pyrrole polymerization in human porphobilinogen deaminase.

Biochim Biophys Acta 2018 Jun 14. Epub 2018 Jun 14.

Protein Stability and Inherited Disease Laboratory, CIC bioGUNE, Derio, Bizkaia, 48160, Spain. Electronic address:

Human porphobilinogen deaminase (PBGD), the third enzyme in the heme pathway, catalyzes four times a single reaction to convert porphobilinogen into hydroxymethylbilane. Remarkably, PBGD employs a single active site during the process, with a distinct yet chemically equivalent bond formed each time. The four intermediate complexes of the enzyme have been biochemically validated and they can be isolated but they have never been structurally characterized other than the apo- and holo-enzyme bound to the cofactor. Read More

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TAG synthesis and storage under osmotic stress. A requirement for preserving membrane homeostasis in renal cells.

Biochim Biophys Acta 2018 Jun 14;1863(9):1108-1120. Epub 2018 Jun 14.

Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Departamento de Ciencias Biológicas, Cátedra de Biología Celular y Molecular, Buenos Aires, Argentina; Universidad de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Química y Fisicoquímica Biológicas Prof. Dr. Alejandro C. Paladini (IQUIFIB)-Facultad de Farmacia y Bioquímica, Buenos Aires, Argentina. Electronic address:

Hyperosmolarity is a controversial signal for renal cells. It can induce cell stress or differentiation and both require an active lipid metabolism. We showed that hyperosmolarity upregulates phospholipid (PL) de novo synthesis in renal cells. Read More

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June 2018
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Cell proliferation and anti-apoptosis: Essential processes for recruitment of the full thermogenic capacity of brown adipose tissue.

Biochim Biophys Acta 2018 Jun 13. Epub 2018 Jun 13.

Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, Stockholm, Sweden.

In mice living under normal animal house conditions, the brown adipocytes in classical brown adipose tissue depots are already essentially fully differentiated: UCP1 mRNA and UCP1 protein levels are practically saturated. This means that any further recruitment - in response to cold exposure or any other browning agent - does not result in significant augmentation of these parameters. This may easily be construed to indicate that classical brown adipose tissue cannot be further recruited. Read More

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G ganglioside-enriched extracellular vesicles stimulate melanocyte migration.

Biochim Biophys Acta 2018 Jun 13. Epub 2018 Jun 13.

Center for Translational Research in Oncology (LIM-24), Instituto do Câncer do Estado de São Paulo, Faculdade de Medicina, Universidade de São Paulo, 01246-000 São Paulo, SP, Brazil. Electronic address:

Melanomas often accumulate gangliosides, sialic acid-containing glycosphingolipids found in the outer leaflet of plasma membranes, as disialoganglioside G and its derivatives. Here, we have transfected the G synthase gene (ST8Sia I) in a normal melanocyte cell line in order to evaluate changes in the biological behavior of non-transformed cells. G-synthase expressing cells converted G into G and accumulated both G and its acetylated form, 9-O-acetyl-G. Read More

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Locating macromolecules and determining structures inside bacterial cells using electron cryotomography.

Biochim Biophys Acta 2018 Jun 13. Epub 2018 Jun 13.

Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom. Electronic address:

Electron cryotomography (cryo-ET) is an imaging technique uniquely suited to the study of bacterial ultrastructure and cell biology. Recent years have seen a surge in structural and cell biology research on bacteria using cryo-ET. This research has driven major technical developments in the field, with applications emerging to address a wide range of biological questions. Read More

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Telomere length alterations in microsatellite stable colorectal cancer and association with the immune response.

Biochim Biophys Acta 2018 Jun 13. Epub 2018 Jun 13.

Unit of Biomarkers and Susceptibility, Cancer Prevention and Control Program, Catalan Institute of Oncology (ICO), Oncobell Program, Bellvitge Biomedical Research Institute (IDIBELL) and CIBERESP, L'Hospitalet de Llobregat, Barcelona, Spain; Department of Clinical Sciences, Faculty of Medicine, University of Barcelona, Barcelona, Spain. Electronic address:

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Nucleic acids binding strategies of small molecules: Lessons from alkaloids.

Biochim Biophys Acta 2018 Jun 13. Epub 2018 Jun 13.

Biophysical Chemistry Laboratory, Organic and Medicinal Chemistry Division, CSIR-Indian Institute of Chemical Biology, Kolkata 700 032, India. Electronic address:

Background: Nucleic acids are now important targets for therapeutic intervention. Alkaloids are an important class of molecules that have myriad therapeutic utility. Isoquinoline and benzophenanthridine alkaloids exhibit multiple pharmacological activities which are often related to their strong nucleic acid binding abilities. Read More

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SIRT2 reduces actin polymerization and cell migration through deacetylation and degradation of HSP90.

Biochim Biophys Acta 2018 Jun 14;1865(9):1230-1238. Epub 2018 Jun 14.

Department of Microbiology & Molecular Biology, College of Biological Science and Biotechnology, Chungnam National University, Daejeon 34134, Republic of Korea. Electronic address:

SIRT2, a member of the class III histone deacetylase family, has been identified as a tumor suppressor, which is associated with various cellular processes including metabolism and proliferation. However, the effects of SIRT2 on cancer cell migration caused by cytoskeletal rearrangement remain uncertain. Here we show that SIRT2 inhibits cell motility by suppressing actin polymerization. Read More

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June 2018
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Lipidomic adaptations of the Metarhizium robertsii strain in response to the presence of butyltin compounds.

Biochim Biophys Acta 2018 Jun 14. Epub 2018 Jun 14.

Department of Industrial Microbiology and Biotechnology, Faculty of Biology and Environmental Protection, University of Lodz, Banacha 12/16, 90-237 Lodz, Poland. Electronic address:

Metarhizium robertsii, a butyltin-resistant filamentous fungus, can rapid and complete biodegradation of di- (DBT) and tributyltin (TBT) under conditions of intensive aeration and ascorbic acid supplementation. In this paper, lipidomic investigations were performed to find the membrane adaptations necessary for effective butyltins degradation. HPLC-MS/MS analysis showed that the phospholipid profile was greatly modified during M. Read More

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June 2018
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Fatty acid-binding protein 5 (FABP5) promotes lipolysis of lipid droplets, de novo fatty acid (FA) synthesis and activation of nuclear factor-kappa B (NF-κB) signaling in cancer cells.

Biochim Biophys Acta 2018 Jun 12;1863(9):1057-1067. Epub 2018 Jun 12.

Department of Bioscience and Food Production Science, Interdisciplinary Graduate School of Science and Technology, Shinshu University, Japan; Department of Biomedical Engineering, Graduate School of Science and Technology, Shinshu University, Japan; Department of Bioscience & Biotechnology, Shinshu University, Japan; Department of Interdisciplinary Genome Sciences and Cell Metabolism, Institute for Biomedical Sciences, Interdisciplinary Cluster for Cutting-Edge Research, Shinshu University, Minami-minowa, Kami-ina, Nagano 399-4598, Japan. Electronic address:

Fatty acid-binding proteins (FABPs) are involved in binding and storing hydrophobic ligands such as long-chain fatty acids, as well as transporting them to the appropriate compartments in the cell. Epidermal fatty acid-binding protein (FABP5) is an intracellular lipid-binding protein that is abundantly expressed in adipocytes and macrophages. Previous studies have revealed that the FABP5 expression level is closely related to malignancy in various types of cancer. Read More

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Insights into the RNA quadruplex binding specificity of DDX21.

Biochim Biophys Acta 2018 Jun 12. Epub 2018 Jun 12.

Department of Chemistry, University of Manitoba, Winnipeg, Manitoba, Canada; Department of Biochemistry & Medical Genetics, University of Manitoba, Winnipeg, Manitoba, Canada; Manitoba Institute for Materials, University of Manitoba, Winnipeg, Manitoba, Canada. Electronic address:

Guanine quadruplexes can form in both DNA and RNA and influence many biological processes through various protein interactions. The DEAD-box RNA helicase protein DDX21 has been shown to bind and remodel RNA quadruplexes but little is known about its specificity for different quadruplex species. Previous reports have suggested DDX21 may interact with telomeric repeat containing RNA quadruplex (TERRA), an integral component of the telomere that contributes to telomeric heterochromatin formation and telomere length regulation. Read More

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Pathophysiological consequences of isoform-specific IP receptor mutations.

Biochim Biophys Acta 2018 Jun 12. Epub 2018 Jun 12.

KU Leuven, Laboratory for Molecular and Cellular Signaling, Department of Cellular and Molecular Medicine, Leuven Kanker Instituut, Campus Gasthuisberg O/N-1 B-802, Herestraat 49, BE-3000 Leuven, Belgium. Electronic address:

Ca signaling governs a diverse range of cellular processes and, as such, is subject to tight regulation. A main component of the complex intracellular Ca-signaling network is the inositol 1,4,5-trisphosphate (IP) receptor (IPR), a tetrameric channel that mediates Ca release from the endoplasmic reticulum (ER) in response to IP. IPR function is controlled by a myriad of factors, such as Ca, ATP, kinases and phosphatases and a plethora of accessory and regulatory proteins. Read More

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Corrigendum to "Interleukin-1β and tumor necrosis factor-α augment acidosis-induced rat articular chondrocyte apoptosis via nuclear factor-kappaB-dependent upregulation of ASIC1a channel" [Biochimica et Biophysica Acta 1864/1 (2018) 162-177].

Biochim Biophys Acta 2018 Jun 11. Epub 2018 Jun 11.

Anhui Key Laboratory of Bioactivity of Natural Products, School of Pharmacy, Anhui Medical University, Hefei 230032, China; The Key Laboratory of Anti-inflammatory and Immune Medicine, Anhui Medical University, Ministry of Education, Hefei 230032, China. Electronic address:

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Diacylglycerol acyltransferase-2 contains a c-terminal sequence that interacts with lipid droplets.

Biochim Biophys Acta 2018 Jun 11;1863(9):1068-1081. Epub 2018 Jun 11.

Department of Biochemistry, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E5, Canada. Electronic address:

Diacylglycerol acyltranferase-2 (DGAT2) is a resident protein of the endoplasmic reticulum that catalyzes the synthesis of triacylglycerol. When lipid droplet formation is stimulated by incubating cells with fatty acids, DGAT2 becomes concentrated around the surface of cytosolic lipid droplets. Using confocal microscopy and directed mutagenesis, we have identified a 17-amino acid sequence in the C-terminal region of DGAT2 that is necessary and sufficient for targeting DGAT2 to lipid droplets. Read More

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Profiling of phosphoinositide molecular species in human and mouse platelets identifies new species increasing following stimulation.

Biochim Biophys Acta 2018 Jun 11. Epub 2018 Jun 11.

INSERM U1048, I2MC, Université Paul Sabatier, 31432 Toulouse, France; CHU de Toulouse, Laboratoire d'Hématologie, 31059 Toulouse Cedex 03, France. Electronic address:

Phosphoinositides are bioactive lipids essential in the regulation of cell signaling as well as cytoskeleton and membrane dynamics. Their metabolism is highly active in blood platelets where they play a critical role during activation, at least through two well identified pathways involving phospholipase C and phosphoinositide 3-kinases (PI3K). Here, using a sensitive high-performance liquid chromatography-mass spectrometry method recently developed, we monitored for the first time the profiling of phosphatidylinositol (PI), PIP, PIP and PIP molecular species (fatty-acyl profiles) in human and mouse platelets during the course of stimulation by thrombin and collagen-related peptide. Read More

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Resolvin E1 and its precursor 18R-HEPE restore mitochondrial function in inflammation.

Biochim Biophys Acta 2018 Jun 12;1863(9):1016-1028. Epub 2018 Jun 12.

Department of Internal Medicine II, University Hospital of Giessen, Universities of Giessen and Marburg Lung Center, Giessen, Germany.

Inflammatory disorders such as sepsis are a major cause of morbidity and mortality. Mitochondrial dysfunction is considered a key factor in the pathogenesis of severe inflammation. In the present study, we aimed to investigate the impact of arachidonic acid, omega-3 (n-3) fatty acids, and n-3-derived lipid mediators 18R-HEPE and resolvin (Rv) E1 on mitochondrial function in experimental inflammation. Read More

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Antioxidant and pro-oxidant actions of resveratrol on human serum albumin in the presence of toxic diabetes metabolites: Glyoxal and methyl-Glyoxal.

Biochim Biophys Acta 2018 Jun 11. Epub 2018 Jun 11.

IPROCAR Research Institute, Food Technology, University of Extremadura, 10003, Cáceres. Spain. Electronic address:

Methylglyoxal (MGO) and glyoxal (GO) are attracting considerable attention because of their role in the onset of diabetes symptoms. Therefore, to comprehend the molecular fundamentals of their pathological actions is of the utmost importance. In this study, the molecular interactions between resveratrol (RES) and human serum albumin (HSA) and the ability of the stilbene to counteract the oxidative damage caused by pathological concentrations of MGO and GO to the human plasma protein, was assessed. Read More

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Exploring the major cross-talking edges of competitive endogenous RNA networks in human Chronic and Acute Myeloid Leukemia.

Biochim Biophys Acta 2018 Jun 15;1862(9):1883-1892. Epub 2018 Jun 15.

Bioinformatics Centre, Bose Institute, Kolkata 700 054, India. Electronic address:

Background: Human Chronic and Acute Myeloid Leukemia are myeloproliferative disorders in myeloid lineage of blood cells characterized by accumulation of aberrant white blood cells. In cancer, the anomalous transcriptome includes deregulated expression of non-coding RNAs in conjunction with protein-coding mRNAs in human genome. The coding or non-coding RNA transcripts harboring miRNA-binding sites can converse with and regulate each other by explicitly contending for a limited pool of shared miRNAs and act as competitive endogenous RNAs (ceRNAs). Read More

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