104,316 results match your criteria Biochimica et Biophysica Acta[Journal]


A pericellular hyaluronan matrix is required for the morphological maturation of cortical neurons.

Biochim Biophys Acta Gen Subj 2020 Jul 2:129679. Epub 2020 Jul 2.

Graduate School of Bioagricultural Sciences, Nagoya University, Furocho, Chikusa-Ku, Nagoya 464-8601, Japan; Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwaicho, Fuchu, Tokyo 183-8509, Japan. Electronic address:

Background: Hyaluronan (HA) is a major component of the extracellular matrix (ECM) and is involved in many cellular functions. In the adult brain, HA forms macromolecular aggregates around synapses and plays important roles in neural plasticity. In contrast to the well-characterized function of HA in the adult brain, its roles in the developing brain remain largely unknown. Read More

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http://dx.doi.org/10.1016/j.bbagen.2020.129679DOI Listing

Identification of the electron donor to flavodiiron proteins in Synechocystis sp. PCC 6803 by in vivo spectroscopy.

Biochim Biophys Acta Bioenerg 2020 Jul 2:148256. Epub 2020 Jul 2.

Department of Biological and Environmental Sciences, Faculty of Agriculture, Kobe University, 1-1 Rokkodai, Nada, Kobe 657-8501, Japan.

Flavodiiron proteins (FDPs) of photosynthetic organisms play a photoprotective role by reducing oxygen to water and thus avoiding the accumulation of excess electrons on the photosystem I (PSI) acceptor side under stress conditions. In Synechocystis sp. PCC 6803 grown under high CO, both FDPs Flv1 and Flv3 are indispensable for oxygen reduction. Read More

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http://dx.doi.org/10.1016/j.bbabio.2020.148256DOI Listing

The "discovery" of lipid droplets: A brief history of organelles hidden in plain sight.

Biochim Biophys Acta Mol Cell Biol Lipids 2020 Jul 1:158762. Epub 2020 Jul 1.

Department of Nutrition, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, United States of America. Electronic address:

Mammalian lipid droplets (LDs), first described as early as the 1880s, were virtually ignored for more than 100 years. Between 1991 and the early 2000s, however, a series of discoveries and conceptual breakthroughs led to a resurgent interest in obesity as a disease, in the metabolism of intracellular triacylglycerol (TAG), and in the physical locations of LDs as cellular structures with their associated proteins. Insights included the recognition that obesity underlies major chronic diseases, that appetite is hormonally controlled, that hepatic steatosis is not a benign finding, and that diabetes might fundamentally be a disorder of lipid metabolism. Read More

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http://dx.doi.org/10.1016/j.bbalip.2020.158762DOI Listing

Metformin Preferentially Provides Neuroprotection Following Cardiac Ischemia/Reperfusion in Non-Diabetic Rats.

Biochim Biophys Acta Mol Basis Dis 2020 Jul 1:165893. Epub 2020 Jul 1.

Cardiac Electrophysiology Research and Training Center, Faculty of Medicine, Chiang Mai University, Chiang Mai, 50200, Thailand; Center of Excellence in Cardiac Electrophysiology Research, Chiang Mai University, Chiang Mai, 50200, Thailand; Department of Oral Biology and Diagnostic Sciences, Faculty of Dentistry, Chiang Mai University, Chiang Mai, 50200, Thailand. Electronic address:

Following acute myocardial infarction, re-establishment of coronary perfusion aggravates further injuries in the heart and remote organs including the brain as a consequence of ischemia/reperfusion (I/R) injury. Since pretreatment with metformin attenuated both cardiac and cerebral I/R injury via AMP-activated protein kinase (AMPK) pathways, we hypothesized that metformin given after ischemia mitigates both cardiac and brain pathologies following cardiac I/R. Male Wistar rats were subjected to either cardiac I/R (30 min-ischemia/120 min-reperfusion; n=30) or sham operation (n=5). Read More

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http://dx.doi.org/10.1016/j.bbadis.2020.165893DOI Listing

RNA and gene control in bacteria.

Biochim Biophys Acta Gene Regul Mech 2020 Jul 1:194602. Epub 2020 Jul 1.

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http://dx.doi.org/10.1016/j.bbagrm.2020.194602DOI Listing

Human Mpv17-like protein with a mitigating effect on mtDNA damage is involved in cAMP/PKA signaling in the mitochondrial matrix.

Biochim Biophys Acta Mol Cell Res 2020 Jul 1:118792. Epub 2020 Jul 1.

Life Science Innovation Center, University of Fukui, Fukui 910-1193, Japan.

Human Mpv17-like protein (M-LPH/Mpv17L) is thought to play a role in minimizing mitochondrial dysfunction caused by mitochondrial DNA (mtDNA) damage. We have recently demonstrated that, in addition to an increase of mtDNA damage, M-LPH-knockout (M-LPH-KO) in HepG2 cells causes a significant reduction of mitochondrial transcription factor A (TFAM) protein, an essential factor for mtDNA maintenance, along with an increase in its phosphorylation. These intracellular changes suggested an association of M-LPH with the cAMP/PKA signaling pathway, as selective degradation of TFAM by mitochondrial protease is driven by protein kinase A (PKA)-dependent phosphorylation. Read More

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http://dx.doi.org/10.1016/j.bbamcr.2020.118792DOI Listing

Eif2s3y regulates the proliferation of spermatogonial stem cells via Wnt6/-catenin signaling pathway.

Biochim Biophys Acta Mol Cell Res 2020 Jul 1:118790. Epub 2020 Jul 1.

College of Veterinary Medicine, Shaanxi Center of Stem Cells Engineering & Technology, Northwest A & F University, Yangling, Shaanxi, China. Electronic address:

Eukaryotic translation initiation factor 2 subunit 3 and structural gene Y-linked (Eif2s3y) gene, the gene encoding eIF2γ protein, is globally expressed in all tissues and plays important roles in regulating global and gene-specific mRNA translation initiation. It has been noticed that Eif2s3y plays crucial roles in spermatogenesis, however, the mechanism remains unclear. In the current study, transgenic Eif2s3y mice were generated to test our hypothesis that the Eif2s3y promotes the proliferation of spermatogonial stem cells (SSCs). Read More

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http://dx.doi.org/10.1016/j.bbamcr.2020.118790DOI Listing

Transient depletion of transported metabolites in the streaming cytoplasm of Chara upon shading the long-distance transmission pathway.

Biochim Biophys Acta Bioenerg 2020 Jul 1:148257. Epub 2020 Jul 1.

Department of Biophysics, Faculty of Biology, Moscow State University, Moscow 119991, Russia. Electronic address:

Export of reducing power from chloroplasts to cytoplasm serves to balance the NADPH/ATP ratio that is optimal for CO assimilation. Rapid cytoplasmic streaming in characean algae conveys the exported metabolites downstream towards the shaded plastids where envelope transporters may operate for the import of reducing power in accordance with the direction of concentration gradients. Import of reducing equivalents by chloroplasts in the analyzed area transiently enhances the pulse-modulated chlorophyll fluorescence F' controlled by the redox state of photosystem II acceptor Q. Read More

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http://dx.doi.org/10.1016/j.bbabio.2020.148257DOI Listing

Ryanodine receptor 1 mediated dexamethasone-induced chondrodysplasia in fetal rats.

Biochim Biophys Acta Mol Cell Res 2020 Jun 30:118791. Epub 2020 Jun 30.

Department of Pharmacology, Basic Medical School of Wuhan University, Wuhan 430071, China; Hubei Provincial Key Laboratory of Developmentally Originated Disease, Wuhan, 430071, China. Electronic address:

Background: Osteoarthritis is caused by cartilage dysplasia and has fetal origin. Prenatal dexamethasone exposure (PDE) induced chondrodysplasia in fetal rats by inhibiting transforming growth factor β (TGFβ) signaling. This study aimed to determine the effect of dexamethasone on fetal cartilage development and illustrate the underlying molecular mechanism. Read More

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http://dx.doi.org/10.1016/j.bbamcr.2020.118791DOI Listing

Regulation of light-induced H extrusion and uptake by cyanobacterial homologs of the plastidial FLAP1, DLDG1, and Ycf10 in Synechocystis sp. PCC6803.

Biochim Biophys Acta Bioenerg 2020 Jun 30:148258. Epub 2020 Jun 30.

Department of Life Science and Technology, Tokyo Institute of Technology, Yokohama 226-8501, Japan. Electronic address:

Upon a dark-to-light transition, multiple species of cyanobacteria release a certain amount of H from the inside to the outside of their cells. Previous studies revealed the plasma membrane-localizing Proton exchange A (PxcA) is involved in the light-induced H extrusion in the cyanobacterium Synechocystis sp. PCC6803. Read More

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http://dx.doi.org/10.1016/j.bbabio.2020.148258DOI Listing

State transitions in cyanobacteria studied with picosecond fluorescence at room temperature.

Biochim Biophys Acta Bioenerg 2020 Jun 30:148255. Epub 2020 Jun 30.

Laboratory of Biophysics, Wageningen University, Wageningen, the Netherlands; MicroSpectroscopy Research Facility, Wageningen University, Wageningen, the Netherlands. Electronic address:

Cyanobacteria can rapidly regulate the relative activity of their photosynthetic complexes photosystem I and II (PSI and PSII) in response to changes in the illumination conditions. This process is known as state transitions. If PSI is preferentially excited, they go to state I whereas state II is induced either after preferential excitation of PSII or after dark adaptation. Read More

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http://dx.doi.org/10.1016/j.bbabio.2020.148255DOI Listing

Influence of cytosolic conditions on the reaction equilibrium and the reaction enthalpy of the enolase reaction accessed by calorimetry and van 't HOFF.

Biochim Biophys Acta Gen Subj 2020 Jun 28:129675. Epub 2020 Jun 28.

UFZ - Helmholtz Centre for Environmental Research, Dept. Environmental Microbiology, Permoserstr. 15, D-04318 Leipzig, Germany. Electronic address:

Background: Thermodynamic methods are finding more and more applications in systems biology, which attempts to understand cell functions mechanistically. Unfortunately, the state variables used (reaction enthalpy and Gibbs energy) do not take sufficient account of the conditions inside of cells, especially the crowding with macromolecules.

Methods: For this reason, the influence of crowding agents and various other parameters such as salt concentrations, pH and temperature on equilibrium position and reaction enthalpy of the glycolytic example reaction 9 (2-Phospoglycerate - > Phosphoenolpyruvate + HO) was investigated. Read More

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http://dx.doi.org/10.1016/j.bbagen.2020.129675DOI Listing

Sp4 controls constitutive expression of neuronal serine racemase and NF-E2-related factor-2 mediates its induction by valproic acid.

Biochim Biophys Acta Gene Regul Mech 2020 Jun 27;1863(9):194597. Epub 2020 Jun 27.

School of Optometry and Ophthalmology and the Eye Hospital, Wenzhou Medical University, China; State Key Laboratory of Optometry, Ophthalmology, and Visual Science, 270 Xueyuan Road, Wenzhou, Zhejiang 325003, China. Electronic address:

Serine racemase (SR) synthesizes l-type serine to its enantimor, d-serine which participates in physiological processes and in pathological conditions. In the central nervous system, SR is highly expressed in neurons and astrocytes but expressed at relatively lower amount in microglia. However, the mechanism by which SR is highly expessed in neurons is hitherto unknown. Read More

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http://dx.doi.org/10.1016/j.bbagrm.2020.194597DOI Listing

SARS-CoV-2 pathophysiology and assessment of coronaviruses in CNS diseases with a focus on therapeutic targets.

Biochim Biophys Acta Mol Basis Dis 2020 Jun 27:165889. Epub 2020 Jun 27.

Professor of Internal Medicine, Texas Tech University Health Sciences Center, Lubbock, TX, USA; Department of Biochemistry, Kakatiya Medical College, Warangal 506007, Telangana, India; Applied Biology, CSIR-Indian Institute of Technology, Uppal Road, Tarnaka, Hyderabad 500007, Telangana, India. Electronic address:

The novel Coronavirus disease of 2019 (nCOV-19) is a viral outbreak noted first in Wuhan, China. This disease is caused by Severe Acute Respiratory Syndrome (SARS) Coronavirus (CoV)-2. In the past, other members of the coronavirus family, such as SARS and Middle East Respiratory Syndrome (MERS), have made an impact in China and the Arabian peninsula respectively. Read More

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http://dx.doi.org/10.1016/j.bbadis.2020.165889DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7320676PMC

Role of calpains in promoting desmin filaments depolymerization and muscle atrophy.

Authors:
Shenhav Cohen

Biochim Biophys Acta Mol Cell Res 2020 Jun 27;1867(10):118788. Epub 2020 Jun 27.

Technion Israel Institute of Technology, Israel. Electronic address:

Muscle atrophy is an inevitable sequel of fasting, denervation, aging, exposure to microgravity, and many human diseases including, cancer, type-2 diabetes, and renal failure. During atrophy the destruction of the muscle's fundamental contractile machinery, the myofibrils, is accelerated leading to a reduction in muscle mass, weakness, frailty, and physical disability. Recent findings indicate that atrophy can be a major cause of death in affected individuals, and inhibition of muscle wasting is likely to prolong survival. Read More

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http://dx.doi.org/10.1016/j.bbamcr.2020.118788DOI Listing

Characterization of an NAD(P)-dependent meso-diaminopimelate dehydrogenase from Thermosyntropha lipolytica.

Biochim Biophys Acta Proteins Proteom 2020 Jun 26:140476. Epub 2020 Jun 26.

Research Institute for Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), 3-11-32 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-0046, Japan. Electronic address:

meso-Diaminopimelate dehydrogenase (meso-DAPDH) catalyzes the reversible NADP-dependent oxidative deamination of meso-2,6-diaminopimelate (meso-DAP) to produce L-2-amino-6-oxopimelate. meso-DAPDH is divided into two major clusters, types I and II, based on substrate specificity and structural characteristic. Here, we describe a novel type II meso-DAPDH from Thermosyntropha lipolytica (TlDAPDH). Read More

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http://dx.doi.org/10.1016/j.bbapap.2020.140476DOI Listing

Post-translational modifications contribute to neoepitopes in Type-1 diabetes: Challenges for inducing antigen-specific tolerance.

Biochim Biophys Acta Proteins Proteom 2020 Jun 26;1868(10):140478. Epub 2020 Jun 26.

Department of Microbiology and Immunology, University of Illinois-College of Medicine, Chicago, IL, United States of America. Electronic address:

Type-1 Diabetes (T1D) is the major autoimmune disease affecting the juvenile population in which insulin-producing pancreatic β-cells are destroyed by self-reactive T-cells and B-cells. Emerging studies have identified the presence of autoantibodies and altered T-cell reactivity against several autoantigens in individuals who are at risk of developing T1D even before the clinical onset of diabetes. Whilst these findings could lead to the development of predictive biomarkers for early diagnosis, growing evidence on the generation of neoepitopes, epitope spreading and diverse antigen repertoire in T1D poses a major challenge for developing approaches to induce antigen-specific tolerance. Read More

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http://dx.doi.org/10.1016/j.bbapap.2020.140478DOI Listing

Tyr-nitration in maize CDKA;1 results in lower affinity for ATP binding.

Biochim Biophys Acta Proteins Proteom 2020 Jun 26;1868(10):140479. Epub 2020 Jun 26.

Universidad de Buenos Aires - Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Química y Fisicoquímica Biológicas "Profesor Alejandro C. Paladini" (IQUIFIB), Facultad de Farmacia y Bioquímica, Buenos Aires, Argentina. Electronic address:

Cyclin-dependent kinase A (CDKA) is a key component for cell cycle progression. The catalytic kinase activity depends on the protein's ability to form an active complex with cyclins and on phosphoregulatory mechanisms. Cell cycle arrest and plant growth impairment under abiotic stress have been linked to different molecular processes triggered by increased levels of reactive oxygen and nitrogen species (ROS and RNS). Read More

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http://dx.doi.org/10.1016/j.bbapap.2020.140479DOI Listing

Influence of the conformations of αA-crystallin peptides on the isomerization rates of aspartic acid residues.

Biochim Biophys Acta Proteins Proteom 2020 Jun 26;1868(10):140480. Epub 2020 Jun 26.

Graduate School of Pharmacy, Meijo University, 150 Yagotoyama, Tempaku-ku, Nagoya, Aichi 468-8503, Japan; Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, Kakuma-machi, Kanazawa, Ishikawa 920-1192, Japan; Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Suita, Osaka 565-0871, Japan. Electronic address:

The isomerization rate of aspartic acid (Asp) residue is known to be affected by the three-dimensional structures of peptides and proteins. Although the isomerized Asp residues were experimentally observed, structural features which affect the isomerization cannot be elucidated sufficiently because of protein denaturation and aggregation. In this study, molecular dynamics (MD) simulations were conducted on three αA-crystallin peptides (T6, T10, and T18), each containing a single Asp residue with different isomerization rate (T18 > T6 > T10) to clarify the structural factors of Asp isomerization tendency. Read More

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http://dx.doi.org/10.1016/j.bbapap.2020.140480DOI Listing

The circadian nuclear receptor RORα negatively regulates cerebral ischemia-reperfusion injury and mediates the neuroprotective effects of melatonin.

Biochim Biophys Acta Mol Basis Dis 2020 Jun 26:165890. Epub 2020 Jun 26.

Department of Cardiology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China. Electronic address:

Disruptions of the circadian rhythm and reduced circulating levels of the circadian hormone melatonin predispose to ischemic stroke. Although the nuclear receptor RORα is considered as a circadian rhythm regulator and a mediator of certain melatonin effects, its potential role in cerebral ischemia-reperfusion (CI/R) injury and in the neuroprotective effects of melatonin remain undefined. Here, we observed that CI/R injury in RORα-deficient mice was associated with greater cerebral infarct size, brain edema, and cerebral apoptosis compared with wild-type model. Read More

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http://dx.doi.org/10.1016/j.bbadis.2020.165890DOI Listing

Oxidative stress abrogates the degradation of KMT2D to promote degeneration in nucleus pulposus.

Biochim Biophys Acta Mol Basis Dis 2020 Jun 26;1866(10):165888. Epub 2020 Jun 26.

Department of Orthopaedic Surgery, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China; Key Laboratory of Musculoskeletal System Degeneration and Regeneration Translational Research of Zhejiang Province, Hangzhou, Zhejiang, China. Electronic address:

Nucleus pulposus (NP) degeneration plays pivotal roles in intervertebral disc degeneration. The effect and mechanism of oxidative stress and epigenetics in NP degeneration is still unclear. We performed this study to evaluate the function of oxidative stress in NP and to explore the potential mechanism of ROS induced expression of matrix metalloproteinases (MMPs). Read More

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http://dx.doi.org/10.1016/j.bbadis.2020.165888DOI Listing

Intermediary metabolism: An intricate network at the crossroads of cell fate and function.

Biochim Biophys Acta Mol Basis Dis 2020 Jun 26:165887. Epub 2020 Jun 26.

Molecular Physical Chemistry Research Unit (QFM-UC), Center of Investigation in Environment, Genetics and Oncobiology (CIMAGO) and Department of Life Sciences, University of Coimbra, 3000-456 Coimbra, Portugal. Electronic address:

Intermediary metabolism is traditionally viewed as the large, highly integrated network of reactions that provides cells with metabolic energy, reducing power and biosynthetic intermediates. The elucidation of its major pathways and molecular mechanisms of energy transduction occupied some of the brightest scientific minds for almost two centuries. When these goals were achieved, a sense that intermediary metabolism was mostly a solved problem pervaded the broader biochemical community, and the field lost its vitality. Read More

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http://dx.doi.org/10.1016/j.bbadis.2020.165887DOI Listing
June 2020
4.882 Impact Factor

Chromatin remodeling and transcription of the TPK1 subunit of PKA during stress in Saccharomyces cerevisiae.

Biochim Biophys Acta Gene Regul Mech 2020 Jun 26:194599. Epub 2020 Jun 26.

Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento Química Biológica, Buenos Aires, Argentina; CONICET- Universidad de Buenos Aires, Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN), Buenos Aires, Argentina. Electronic address:

In response to environmental changes cells rapidly rearrange their gene expression pattern in order to adapt to the new conditions. Chromatin remodeling is critical for this process playing a major role in the induction of genes involved in stress responses. We demonstrated previously that TPK1, encoding one of the catalytic subunits of PKA from Saccharomyces cerevisiae, is upregulated under heat shock. Read More

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http://dx.doi.org/10.1016/j.bbagrm.2020.194599DOI Listing

The emerging roles of GCN5L1 in mitochondrial and vacuolar organelle biology.

Biochim Biophys Acta Gene Regul Mech 2020 Jun 26:194598. Epub 2020 Jun 26.

Cardiovascular Branch, National Heart, Lung and Blood Institute, NIH, Bethesda, MD, 20892, USA. Electronic address:

General control of amino acid synthesis 5 like 1 (GCN5L1) was named due to its loose sequence alignment to GCN5, a catalytic subunit of numerous histone N-acetyltransferase complexes. Further studies show that GCN5L1 has mitochondrial and cytosolic isoforms, although functional-domain sequence alignment and experimental studies show that GCN5L1 itself does not possess intrinsic acetyltransferase activity. Nevertheless, GCN5L1 does support protein acetylation in the mitochondria and cytosol and functions as a subunit of numerous intracellular multiprotein complexes that control intracellular vacuolar organelle positioning and function. Read More

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http://dx.doi.org/10.1016/j.bbagrm.2020.194598DOI Listing

Biometals and glycosylation in humans: Congenital disorders of glycosylation shed lights into the crucial role of manganese Golgi homeostasis.

Biochim Biophys Acta Gen Subj 2020 Jun 26:129674. Epub 2020 Jun 26.

Univ. Lille, CNRS, UMR 8576 - UGSF - Unité de Glycobiologie Structurale et Fonctionnelle, F-59000, Lille, France. Electronic address:

About half of the eukaryotic proteins bind biometals that participate in their structure and functions in virtually all physiological processes, including glycosylation. After reviewing the biological roles and transport mechanisms of calcium, magnesium, manganese, zinc and cobalt acting as cofactors of the metalloproteins involved in sugar metabolism and/or glycosylation, the paper will outline the pathologies resulting from a dysregulation of these metals homeostasis and more particularly Congenital Disorders of Glycosylation (CDGs) caused by ion transporter defects. Highlighting of CDGs due to defects in SLC39A8 (ZIP8) and TMEM165, two proteins transporting manganese from the extracellular space to cytosol and from cytosol to the Golgi lumen, respectively, has emphasized the importance of manganese homeostasis for glycosylation. Read More

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http://dx.doi.org/10.1016/j.bbagen.2020.129674DOI Listing

JAK2 regulates Nav1.6 channel function via FGF14 phosphorylation.

Biochim Biophys Acta Mol Cell Res 2020 Jun 26:118786. Epub 2020 Jun 26.

Department of Pharmacology & Toxicology, The University of Texas Medical Branch, Galveston, TX, USA; Center for Addiction Research, The University of Texas Medical Branch, Galveston, TX, USA. Electronic address:

Background: Protein interactions between voltage-gated sodium (Nav) channels and accessory proteins play an essential role in neuronal firing and plasticity. However, a surprisingly limited number of kinases have been identified as regulators of these molecular complexes. We hypothesized that numerous as-of-yet unidentified kinases indirectly regulate the Nav channel via modulation of the intracellular fibroblast growth factor 14 (FGF14), an accessory protein with numerous unexplored phosphomotifs and required for channel function in neurons. Read More

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http://dx.doi.org/10.1016/j.bbamcr.2020.118786DOI Listing

Comparative assessment of purified saponins as permeabilization agents during respirometry.

Biochim Biophys Acta Bioenerg 2020 Jun 26;1861(10):148251. Epub 2020 Jun 26.

Chair of Molecular Nutritional Medicine, TUM School of Life Sciences, Technical University of Munich, Freising, Germany; EKFZ - Else Kröner-Fresenius Center for Nutritional Medicine, Technical University of Munich, Freising, Germany. Electronic address:

Saponins are a diverse group of secondary plant metabolites, some of which display hemolytic toxicity due to plasma membrane permeabilization. This feature is employed in biological applications for transferring hydrophilic molecules through cell membranes. Widely used commercial saponins include digitonin and saponins from soap tree bark, both of which constitute complex mixtures of little definition. Read More

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http://dx.doi.org/10.1016/j.bbabio.2020.148251DOI Listing

Predicting protein subcellular location with network embedding and enrichment features.

Biochim Biophys Acta Proteins Proteom 2020 Jun 25;1868(10):140477. Epub 2020 Jun 25.

School of Life Sciences, Shanghai University, Shanghai 200444, People's Republic of China. Electronic address:

The subcellular location of a protein is highly related to its function. Identifying the location of a given protein is an essential step for investigating its related problems. Traditional experimental methods can produce solid determination. Read More

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http://dx.doi.org/10.1016/j.bbapap.2020.140477DOI Listing

Bacillus subtilis cardiolipin protects its own membrane against surfactin-induced permeabilization.

Biochim Biophys Acta Biomembr 2020 Jun 25:183405. Epub 2020 Jun 25.

Department of Genetics and Microbiology, Faculty of Science, Charles University, Viničná 5, 128 00 Prague, Czech Republic. Electronic address:

Surfactin, a cyclic lipoheptapeptide produced by Bacillus subtilis, is a surface-active antimicrobial that targets the barrier function of lipid membranes. It inserts itself into the membrane, where it forms conductive pores. Depending on its concentration, it eventually disintegrates the membrane in a detergent-like manner. Read More

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http://dx.doi.org/10.1016/j.bbamem.2020.183405DOI Listing

Bibliometric analysis of personalized humanized mouse and Drosophila models for effective combinational therapy in cancer patients.

Biochim Biophys Acta Mol Basis Dis 2020 Jun 24:165880. Epub 2020 Jun 24.

Christian-Albrechts Universität zu Kiel, Zoologisches Institut, Molekulare Physiologie, Olshausenstraße 40, D-24098 Kiel, Germany; German Center for Lung Research, Airway Research Center North, Kiel, Germany. Electronic address:

Research performed using model organisms such as mice and the fruit fly, Drosophila melanogaster has significantly enhanced our knowledge about cancer biology and the fundamental processes of cancer. This is because the major biological properties and genes associated with cancer including signaling pathways, oncogenes, tumor suppressors, and other regulators of cell growth and proliferation are evolutionary conserved. This review provides bibliometric analysis of research productivity, and performance of authors, institutions, countries, and journals associated with personalized animal cancer models, focussing on the role of Drosophila in cancer research, thus highlighting emerging trends in the field. Read More

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http://dx.doi.org/10.1016/j.bbadis.2020.165880DOI Listing

Loss of CLN3, the gene mutated in juvenile neuronal ceroid lipofuscinosis, leads to metabolic impairment and autophagy induction in retina pigment epithelium.

Biochim Biophys Acta Mol Basis Dis 2020 Jun 24:165883. Epub 2020 Jun 24.

Department of Ophthalmology and Visual Sciences, University of Kentucky, Lexington, KY, United States; Markey Cancer Center, University of Kentucky, Lexington, KY, United States. Electronic address:

Juvenile neuronal ceroid lipofuscinosis (JNCL, aka. juvenile Batten disease or CLN3 disease) is a lysosomal storage disease characterized by progressive blindness, seizures, cognitive and motor failures, and premature death. JNCL is caused by mutations in the Ceroid Lipofuscinosis, Neuronal 3 (CLN3) gene, whose function is unclear. Read More

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http://dx.doi.org/10.1016/j.bbadis.2020.165883DOI Listing

Activation of glycogenolysis and glycolysis in breast cancer stem cell models.

Biochim Biophys Acta Mol Basis Dis 2020 Jun 24;1866(10):165886. Epub 2020 Jun 24.

Institute of Molecular Biology and Biophysics, Federal Research Center of Fundamental and Translational Medicine, 630117 Novosibirsk, Russia; Vall D'Hebron Institut de Recerca, 08035 Barcelona, Spain.. Electronic address:

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http://dx.doi.org/10.1016/j.bbadis.2020.165886DOI Listing

Kinetic and protective role of autophagy in manganese-exposed BV-2 cells.

Biochim Biophys Acta Mol Cell Res 2020 Jun 24:118787. Epub 2020 Jun 24.

CONICET, Universidad de Buenos Aires, Instituto de Química Biológica Ciencias Exactas y Naturales (IQUIBICEN), Facultad de Ciencias Exactas y Naturales, Departamento de Química Biológica. Pabellón II, Ciudad Universitaria, 1428 Buenos Aires, Argentina. Electronic address:

Manganese (Mn) plays an important role in many physiological processes. Nevertheless, Mn accumulation in the brain can cause a parkinsonian-like syndrome known as manganism. Unfortunately, the therapeutic options for this disease are scarce and of limited efficacy. Read More

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http://dx.doi.org/10.1016/j.bbamcr.2020.118787DOI Listing

Impact of intercellular connectivity on epithelial mesenchymal transition plasticity.

Biochim Biophys Acta Mol Cell Res 2020 Jun 23:118784. Epub 2020 Jun 23.

School of Medical Science and Technology, Indian Institute of Technology Kharagpur, 721302, West Bengal, India.

Epithelial mesenchymal transition (EMT) in development, tissue repair and carcinogenesis involves cellular plasticity with varying degrees of epithelial and mesenchymal properties. Several recent studies have focused on EMT phenotypic dynamism; however, information on cellular interaction in the context of EMT is inadequate. In our previous study, we investigated EMT phenotypic plasticity and anticipated it as a population driven interactive process. Read More

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http://dx.doi.org/10.1016/j.bbamcr.2020.118784DOI Listing

LGALS1 acts as a pro-survival molecule in AML.

Biochim Biophys Acta Mol Cell Res 2020 Jun 23;1867(10):118785. Epub 2020 Jun 23.

Department of Leukemia, University of Texas MD Anderson Cancer Center, Houston, TX 77030, United States of America; Section of Molecular Hematology and Therapy, University of Texas MD Anderson Cancer Center, Houston, TX 77030, United States of America.

The galectin LGALS1 is a glycan binding protein that regulates intracellular (e.g. signal transduction) and extracellular processes (e. Read More

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http://dx.doi.org/10.1016/j.bbamcr.2020.118785DOI Listing

Bacteria as a double-action sword in cancer.

Biochim Biophys Acta Rev Cancer 2020 Jun 23;1874(1):188388. Epub 2020 Jun 23.

Department of Clinical Oncology, Queen Elizabeth Hospital, Kowloon, Hong Kong, SAR, China. Electronic address:

Bacteria have long been known as one of the primary causative agents of cancer, however, recent studies suggest that they can be used as a promising agent in cancer therapy. Because of the limitations that conventional treatment faces due to the specific pathophysiology and the tumor environment, there is a great need for the new anticancer therapeutic agents. Bacteriotherapy utilizes live, attenuated strains or toxins, peptides, bacteriocins of the bacteria in the treatment of cancer. Read More

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http://dx.doi.org/10.1016/j.bbcan.2020.188388DOI Listing

Post-translational modifications of S1PR1 and endothelial barrier regulation.

Biochim Biophys Acta Mol Cell Biol Lipids 2020 Jun 22:158760. Epub 2020 Jun 22.

Department of Pharmacology and Center for Lung and Vascular Biology, University of Illinois at Chicago Chicago, IL 60126, United States of America. Electronic address:

Sphingosine-1-phosphate receptor-1 (S1PR1), a G-protein coupled receptor that is expressed in endothelium and activated upon ligation by the bioactive lipid sphingosine-1-phosphate (S1P), is an important vascular-barrier protective mechanism at the level of adherens junctions (AJ). Loss of endothelial barrier function is a central factor in the pathogenesis of various inflammatory conditions characterized by protein-rich lung edema formation, such as acute respiratory distress syndrome (ARDS). While several S1PR1 agonists are available, the challenge of arresting the progression of protein-rich edema formation remains to be met. Read More

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http://dx.doi.org/10.1016/j.bbalip.2020.158760DOI Listing

Central administration of β-MSH reduces body weight in obese male Pomc mice.

Biochim Biophys Acta Gen Subj 2020 Jun 22;1864(10):129673. Epub 2020 Jun 22.

Department of Physiology, Faculty of Medical and Health Sciences, University of Auckland, Private Bag 92019, Auckland 1142, New Zealand; Department of Molecular Medicine and Pathology, Faculty of Medical and Health Sciences, University of Auckland, Private Bag 92019, Auckland 1142, New Zealand; Maurice Wilkins Centre for Biodiscovery, University of Auckland, Private Bag 92019, Auckland 1142, New Zealand. Electronic address:

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http://dx.doi.org/10.1016/j.bbagen.2020.129673DOI Listing

H nuclear magnetic resonance spectroscopy supports larger amplitude fast motion and interference with lipid chain ordering for membrane that contains β sheet human immunodeficiency virus gp41 fusion peptide or helical hairpin influenza virus hemagglutinin fusion peptide at fusogenic pH.

Biochim Biophys Acta Biomembr 2020 Jun 23;1862(10):183404. Epub 2020 Jun 23.

Department of Chemistry, Michigan State University, East Lansing, MI 48824, USA. Electronic address:

Enveloped viruses are surrounded by a membrane which is obtained from an infected host cell during budding. Infection of a new cell requires joining (fusion) of the virus and cell membranes. This process is mediated by a monotopic viral fusion protein with a large ectodomain outside the virus. Read More

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http://dx.doi.org/10.1016/j.bbamem.2020.183404DOI Listing

Enhancement of direct membrane penetration of arginine-rich peptides by polyproline II helix structure.

Biochim Biophys Acta Biomembr 2020 Jun 23;1862(10):183403. Epub 2020 Jun 23.

Department of Biophysical Chemistry, Kyoto Pharmaceutical University, 5 Misasagi-Nakauchi-cho, Yamashina-ku, Kyoto 607-8414, Japan.

The left-handed, extended polyproline II (PPII) helix is a unique secondary structure which potently modulates peptide/protein functions through its constraint conformation. To investigate the effect of PPII helix on the direct cell membrane penetration of arginine-rich peptides, we designed a polyproline-containing arginine-rich peptide P9R7W (PPPPPPPPPRRRRRRRW) by introducing nine proline residues into a linear R7W (RRRRRRRW) peptide. Circular dichroism spectroscopy showed that P9R7W has the PPII helix structure in solution whereas R7W is predominantly in random coil structure. Read More

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http://dx.doi.org/10.1016/j.bbamem.2020.183403DOI Listing

Criticality in the conformational phase transition among self-similar groups in intrinsically disordered proteins: Probed by salt-bridge dynamics.

Biochim Biophys Acta Proteins Proteom 2020 Jun 21;1868(10):140474. Epub 2020 Jun 21.

Department of Microbiology, Asutosh College (affiliated to University of Calcutta), Kolkata 700026, India. Electronic address:

Intrinsically disordered proteins (IDP) serve as one of the key components in the global proteome. In contrast to globular proteins, they harbor an enormous amount of physical flexibility enforcing them to be retained in conformational ensembles rather than stable folds. Previous studies in an aligned direction have revealed the importance of transient dynamical phenomena like that of salt-bridge formation in IDPs to support their physical flexibility and have further highlighted their functional relevance. Read More

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http://dx.doi.org/10.1016/j.bbapap.2020.140474DOI Listing

Local and systemic immunosuppression in pancreatic cancer: Targeting the stalwarts in tumor's arsenal.

Biochim Biophys Acta Rev Cancer 2020 Jun 21;1874(1):188387. Epub 2020 Jun 21.

The Eppley Institute for Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, Nebraska 68198, USA; Fred & Pamela Buffett Cancer Center, University of Nebraska Medical Center, Omaha, Nebraska 68198, USA. Electronic address:

Late detection, compromised immune system, and chemotherapy resistance underlie the poor patient prognosis for pancreatic ductal adenocarcinoma (PDAC) patients, making it the 3 leading cause of cancer-related deaths in the United States. Cooperation between the tumor cells and the immune system leads to the immune escape and eventual establishment of the tumor. For more than 20 years, sincere efforts have been made to intercept the tumor-immune crosstalk and identify the probable therapeutic targets for breaking self-tolerance toward tumor antigens. Read More

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http://dx.doi.org/10.1016/j.bbcan.2020.188387DOI Listing

5-Fluorouracil induced dysregulation of the microbiome-gut-brain axis manifesting as depressive like behaviors in rats.

Biochim Biophys Acta Mol Basis Dis 2020 Jun 20;1866(10):165884. Epub 2020 Jun 20.

Department of Radiology, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310003, China. Electronic address:

Disturbances of the gut microbiome have been widely suggested to be associated with 5-fluorouracil (5-Fu) induced digestive pathologies. Furthermore, it has been elucidated that the gut microbiome may play a key role in the pathogenesis of depressive disorders via the microbiota-gut-brain axis. Despite the speculation, there exists no direct evidence proving the causality between disturbances in the gut microbiome induced by 5-Fu and depressive mood dysregulation. Read More

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http://dx.doi.org/10.1016/j.bbadis.2020.165884DOI Listing

Pathways involved in viral oncogenesis: New perspectives from virus-host protein interactomics.

Biochim Biophys Acta Mol Basis Dis 2020 Jun 20;1866(10):165885. Epub 2020 Jun 20.

Department of Bioengineering, Marmara University, Istanbul, Turkey. Electronic address:

Oncogenic viruses are among the apparent causes of cancer-associated mortality. It was estimated that 12% to 15% of human malignancies are linked to oncoviruses. Although modernist strategies and traditional genetic studies have defined host-pathogen interactions of the oncoviruses, their host functions which are critical for the establishment of infection still remain mysterious. Read More

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http://dx.doi.org/10.1016/j.bbadis.2020.165885DOI Listing

Insights into predicting diabetic nephropathy using urinary biomarkers.

Biochim Biophys Acta Proteins Proteom 2020 Jun 20;1868(10):140475. Epub 2020 Jun 20.

College of Life Science and Oceanography, Shenzhen University, Shenzhen 518060, PR China. Electronic address:

Diabetic nephropathy (DN) is a serious complication of diabetes caused by changes in the structure and function of the kidneys. It is important to detect diagnostic biomarkers of DN at an early stage, in which the drug can slow the loss of kidney function and prevent disease progression. In recent years, a variety of biological markers related to DN have been discovered, which is of great significance for predicting the occurrence and development of diseases. Read More

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http://dx.doi.org/10.1016/j.bbapap.2020.140475DOI Listing

The effect of L-theanine and S-ketamine on d-serine cellular uptake.

Biochim Biophys Acta Proteins Proteom 2020 Jun 20;1868(10):140473. Epub 2020 Jun 20.

Department of Pharmacodynamics, Semmelweis University, 4 Nagyvárad tér, Budapest H-1089, Hungary. Electronic address:

Decreased extracellular level of d-Serine (D-Ser), a co-agonist of the N-methyl-d-aspartate (NMDA) receptors was connected to receptor hypofunction in the brain and the related deficit of cognitive functions. Extracellular D-Ser concentration is modulated by ASCT neutral amino acid transporters. L-Theanine (L-Tea), a neutral amino acid component of green tea was reported to improve cognitive functions. Read More

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http://dx.doi.org/10.1016/j.bbapap.2020.140473DOI Listing

NMDA-induced nitric oxide generation and CREB activation in central nervous system is dependent on eukaryotic elongation factor 2 kinase.

Biochim Biophys Acta Mol Cell Res 2020 Jun 20;1867(10):118783. Epub 2020 Jun 20.

Program of Neurosciences, Institute of Biology, Fluminense Federal University, Niterói, RJ, Brazil; Department of Physiology and Pharmacology, Biomedical Institute, Fluminense Federal University, Niterói, RJ, Brazil. Electronic address:

The NMDA receptor is crucial to several functions in CNS physiology and some of its effects are mediated by promoting nitric oxide production from L-arginine and activation of signaling pathways and the transcription factor CREB. Our previous work demonstrated in retinal cells that increasing intracellular free L-arginine levels directly correlates to nitric oxide (NO) generation and can be promoted by protein synthesis inhibition and increase of free L-arginine concentration. Eukaryotic elongation factor 2 kinase (eEF2K), a calcium/calmodulin-dependent kinase, is also known to be activated by NMDA receptors leading to protein synthesis inhibition. Read More

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http://dx.doi.org/10.1016/j.bbamcr.2020.118783DOI Listing

The controversy on the ancestral arsenite oxidizing enzyme; deducing evolutionary histories with phylogeny and thermodynamics.

Biochim Biophys Acta Bioenerg 2020 Jun 20;1861(10):148252. Epub 2020 Jun 20.

Aix-Marseille Univ., CNRS, BIP UMR 7281, FR 3479, IMM, 13402 Marseille Cedex 20, France. Electronic address:

The three presently known enzymes responsible for arsenic-using bioenergetic processes are arsenite oxidase (Aio), arsenate reductase (Arr) and alternative arsenite oxidase (Arx), all of which are molybdoenzymes from the vast group referred to as the Mo/W-bisPGD enzyme superfamily. Since arsenite is present in substantial amounts in hydrothermal environments, frequently considered as vestiges of primordial biochemistry, arsenite-based bioenergetics has long been predicted to be ancient. Conflicting scenarios, however, have been put forward proposing either Arr/Arx or Aio as operating in the ancestral metabolism. Read More

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http://dx.doi.org/10.1016/j.bbabio.2020.148252DOI Listing

Transport of Ca and Ca-dependent permeability transition in heart mitochondria in the early stages of Duchenne muscular dystrophy.

Biochim Biophys Acta Bioenerg 2020 Jun 20;1861(10):148250. Epub 2020 Jun 20.

Mari State University, pl. Lenina 1, Yoshkar-Ola, Mari El 424001, Russia; Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Institutskaya 3, Pushchino, Moscow Region 142290, Russia.

Duchenne muscular dystrophy (DMD) is a progressive skeletal muscle disease that is associated with severe cardiac complications in the late stages. Significant mitochondrial dysfunction is reportedly responsible for the development of cardiomyopathy with age. At the same time, adaptive changes in mitochondrial metabolism in cardiomyocytes were identified in the early stages of DMD. Read More

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http://dx.doi.org/10.1016/j.bbabio.2020.148250DOI Listing

The mitochondrial permeability transition pore: Is it formed by the ATP synthase, adenine nucleotide translocators or both?

Biochim Biophys Acta Bioenerg 2020 Jun 20;1861(10):148249. Epub 2020 Jun 20.

Department of Biochemistry, Facultad de Química, Universidad Nacional Autónoma de México, Mexico City 04510, Mexico. Electronic address:

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http://dx.doi.org/10.1016/j.bbabio.2020.148249DOI Listing