102,485 results match your criteria Biochimica et Biophysica Acta[Journal]


Animal models for hepatocellular carcinoma.

Biochim Biophys Acta Mol Basis Dis 2019 May 10;1865(5):993-1002. Epub 2018 Aug 10.

Centenary Institute, The University of Sydney, Newtown, New South Wales, 2042, Australia; The University of Sydney Faculty of Medicine and Health, New South Wales, 2006, Australia. Electronic address:

Hepatocellular carcinoma (HCC) represents ~90% of all cases of primary liver cancer and occurs predominantly in patients with underlying chronic liver disease and cirrhosis. Establishing appropriate animal models for HCC is required for basic and translational studies, especially the models that can recapitulate one of the human disease settings. Current animal models can be categorized as chemically-induced, genetically-engineered, xenograft, or a combination of these with each other or with a metabolic insult. Read More

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http://dx.doi.org/10.1016/j.bbadis.2018.08.009DOI Listing

Outside the liver box: The gut microbiota as pivotal modulator of liver diseases.

Biochim Biophys Acta Mol Basis Dis 2019 May 6;1865(5):912-919. Epub 2018 Jul 6.

Sahlgrenska Academy, Institute of Medicine, Department of Molecular and Clinical Medicine, Wallenberg Laboratory, University of Gothenburg, S-413 45 Gothenburg, Sweden. Electronic address:

The gut microbiota affects host physiology and has evolved as an important contributor to health and disease. Gut and liver are closely connected and communicate via the portal vein and the biliary system so the liver is constantly exposed to gut-derived bacterial products and metabolites. The intestinal barrier is important for maintaining physical and functional separation between microbes in the gut and the interior of the host and disruption of the barrier function can lead to bacterial translocation and increased leakage of bacterial metabolites. Read More

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http://dx.doi.org/10.1016/j.bbadis.2018.07.004DOI Listing

Animal models of drug-induced liver injury.

Biochim Biophys Acta Mol Basis Dis 2019 May 3;1865(5):1031-1039. Epub 2018 Sep 3.

Dept. of Pharmacology, Toxicology and Therapeutics, University of Kansas Medical Center, Kansas City, KS, USA. Electronic address:

Drug-induced liver injury (DILI) presents unique challenges for consumers, clinicians, and regulators. It is the most common cause of acute liver failure in the US. It is also one of the most common reasons for termination of new drugs during pre-clinical testing and withdrawal of new drugs post-marketing. Read More

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http://dx.doi.org/10.1016/j.bbadis.2018.08.037DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6478394PMC

Tailoring the CRISPR system to transactivate coagulation gene promoters in normal and mutated contexts.

Biochim Biophys Acta Gene Regul Mech 2019 Apr 18. Epub 2019 Apr 18.

Department of Life Sciences and Biotechnology, University of Ferrara, Italy.

Engineered transcription factors (TF) have expanded our ability to modulate gene expression and hold great promise as bio-therapeutics. The first-generation TF, based on Zinc Fingers or Transcription-Activator-like Effectors (TALE), required complex and time-consuming assembly protocols, and were indeed replaced in recent years by the CRISPR activation (CRISPRa) technology. Here, with coagulation F7/F8 gene promoters as models, we exploited a CRISPRa system based on deactivated (d)Cas9, fused with a transcriptional activator (VPR), which is driven to its target by a single guide (sg)RNA. Read More

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http://dx.doi.org/10.1016/j.bbagrm.2019.04.002DOI Listing

Lipid-modulation of membrane insertion and refolding of the apoptotic inhibitor Bcl-xL.

Biochim Biophys Acta Proteins Proteom 2019 Apr 17. Epub 2019 Apr 17.

Department of Biochemistry and Molecular Biology, University of Kansas Medical Center,Kansas City, KS 66160, USA. Electronic address:

Bcl-xL is a member of the Bcl-2 family of apoptotic regulators, responsible for inhibiting the permeabilization of the mitochondrial outer membrane, and a promising anti-cancer target. Bcl-xL exists in the following conformations, each believed to play a role in the inhibition of apoptosis: (a) a soluble folded conformation, (b) a membrane-anchored (by its C-terminal α8 helix) form, which retains the same fold as in solution and (c) refolded membrane-inserted conformations, for which no structural data are available. Previous studies established that in the cell Bcl-xL exists in a dynamic equilibrium between soluble and membranous states, however, no direct evidence exists in support of either anchored or inserted conformation of the membranous state in vivo. Read More

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http://dx.doi.org/10.1016/j.bbapap.2019.04.006DOI Listing
April 2019
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Structural insights into a novel Ca-independent PL-6 alginate lyase from Vibrio OU02 identify the possible subsites responsible for product distribution.

Biochim Biophys Acta Gen Subj 2019 Apr 17. Epub 2019 Apr 17.

MOE Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266235, China. Electronic address:

Alginate lyases have a wide range of industrial applications, such as oligosaccharide preparation, medical treatment, and bioconversion. Therefore, the discovery and characterization of novel alginate lyases are extremely important. PL-6 alginate lyases are classified into two groups: those with a single domain or two domains. Read More

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http://dx.doi.org/10.1016/j.bbagen.2019.04.013DOI Listing

MICAL2 is expressed in cancer associated neo-angiogenic capillary endothelia and it is required for endothelial cell viability, motility and VEGF response.

Biochim Biophys Acta Mol Basis Dis 2019 Apr 17. Epub 2019 Apr 17.

Scuola Superiore Sant'Anna, Institute of Life Sciences, 56124 Pisa, Italy. Electronic address:

The capacity of inducing angiogenesis is a recognized hallmark of cancer cells. The cancer microenvironment, characterized by hypoxia and inflammatory signals, promotes proliferation, migration and activation of quiescent endothelial cells (EC) from surrounding vascular network. Current anti-angiogenic drugs present side effects, temporary efficacy, and issues of primary resistance, thereby calling for the identification of new therapeutic targets. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S09254439193011
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http://dx.doi.org/10.1016/j.bbadis.2019.04.008DOI Listing
April 2019
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A structural prospective for collagen receptors such as DDR and their binding of the collagen fibril.

Biochim Biophys Acta Mol Cell Res 2019 Apr 17. Epub 2019 Apr 17.

Departments of Biology and Biomedical Engineering, Illinois Institute of Technology, Chicago, IL, USA.

The structure of the collagen fibril surface directly effects and possibly assists the management of, collagen receptor interactions. An important class of collagen receptors, the receptor tyrosine kinases of the Discoidin Domain Receptor family (DDR1 and DDR2), are differentially activated by specific collagen types and play important roles in cell adhesion, migration, proliferation, and matrix remodeling. This review discusses their structure and function as it pertains directly to the fibrillar collagen structure with which they interact far more readily than they do with isolated molecular collagen. Read More

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http://dx.doi.org/10.1016/j.bbamcr.2019.04.008DOI Listing

SMP domain proteins in membrane lipid dynamics.

Biochim Biophys Acta Mol Cell Biol Lipids 2019 Apr 16. Epub 2019 Apr 16.

Lee Kong Chian School of Medicine, Nanyang Technological University, 308232, Singapore; Institute of Resource Development and Analysis, Kumamoto University, Kumamoto 860-0811, Japan. Electronic address:

Synaptotagmin-like mitochondrial-lipid-binding (SMP) domain proteins are evolutionarily conserved family of proteins in eukaryotes that localize between the endoplasmic reticulum (ER) and either plasma membrane (PM) or other organelles. They are involved in tethering of these membrane contact sites through interaction with other proteins and membrane lipids. Recent structural and biochemical studies have demonstrated that SMP domain proteins transport a wide variety of lipid species by the ability of the SMP domain to harbor lipids through its unique hydrophobic cavity. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S13881981193005
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http://dx.doi.org/10.1016/j.bbalip.2019.04.007DOI Listing
April 2019
4 Reads

The complexity of a monogenic neurodegenerative disease: More than two decades of therapeutic driven research into Niemann-Pick type C disease.

Biochim Biophys Acta Mol Cell Biol Lipids 2019 Apr 16. Epub 2019 Apr 16.

Department of Biology, Barnard College-Columbia University, New York, NY 10027, United States of America. Electronic address:

Niemann-Pick type C (NP-C) disease is a rare and fatal neurodegenerative disease typified by aberrations in intracellular lipid transport. Cholesterol and other lipids accumulate in the late endosome/lysosome of all diseased cells thereby causing neuronal and visceral atrophy. A cure for NP-C remains elusive despite the extensive molecular advances emanating from the identification of the primary genetic defect in 1997. Read More

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http://dx.doi.org/10.1016/j.bbalip.2019.04.002DOI Listing
April 2019
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Lung cancer-derived extracellular vesicles induced myotube atrophy and adipocyte lipolysis via the extracellular IL-6-mediated STAT3 pathway.

Biochim Biophys Acta Mol Cell Biol Lipids 2019 Apr 16. Epub 2019 Apr 16.

Department of Pathogenic Biology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China. Electronic address:

Cancer-associated cachexia (CAC) constitutes a metabolic dysfunction characterized by systemic inflammation and body weight loss. Muscle atrophy and adipose tissue lipolysis might explain weight loss in CAC. Specific functions of numerous hormones and cytokines derived from tumours can provoke cachexia. Read More

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http://dx.doi.org/10.1016/j.bbalip.2019.04.006DOI Listing
April 2019
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Compromising human skin in vivo and ex vivo to study skin barrier repair.

Biochim Biophys Acta Mol Cell Biol Lipids 2019 Apr 16. Epub 2019 Apr 16.

Department of Drug Delivery Technology, Cluster BioTherapeutics, Leiden Academic Centre for Drug Research, Leiden University, Leiden, the Netherlands. Electronic address:

Ex vivo regenerated stratum corneum (SC) after tape-stripping can be used as a model to study the barrier function of compromised skin. Yet, details about how close the regenerated SC model mimics the lipid properties (e.g. Read More

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http://dx.doi.org/10.1016/j.bbalip.2019.04.005DOI Listing

Biosynthesis of long-chain polyunsaturated fatty acids in the razor clam Sinonovacula constricta: Characterization of four fatty acyl elongases and a novel desaturase capacity.

Biochim Biophys Acta Mol Cell Biol Lipids 2019 Apr 16. Epub 2019 Apr 16.

Key Laboratory of Applied Marine Biotechnology, Ningbo University, Ministry of Education of China, Ningbo, Zhejiang 315211, China. Electronic address:

As an unusual economically important aquaculture species, Sinonovacula constricta possesses high levels of long-chain polyunsaturated fatty acids (LC-PUFA). Previously, our group identified fatty acyl desaturases (Fad) with Δ5 and Δ6 activities in S. constricta, which was the first report of Δ6 Fad in a marine mollusc. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S13881981193005
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http://dx.doi.org/10.1016/j.bbalip.2019.04.004DOI Listing
April 2019
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Metformin ameliorates endotoxemia-induced endothelial pro-inflammatory responses via AMPK-dependent mediation of HDAC5 and KLF2.

Biochim Biophys Acta Mol Basis Dis 2019 Apr 16. Epub 2019 Apr 16.

Department of Critical Care Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, PR China. Electronic address:

Exaggerated endothelial pro-inflammatory response is a hallmark in the early stage of sepsis and contributes to the subsequent tissue injury and organ failure. The anti-inflammatory effects of AMP-activated protein kinase (AMPK) activator metformin in sepsis has been revealed. However, the underlying mechanisms remain not fully understood. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S09254439193012
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http://dx.doi.org/10.1016/j.bbadis.2019.04.009DOI Listing
April 2019
2 Reads

Biophysical characterization of mycobacterial model membranes and their interaction with rifabutin: Towards lipid-guided drug screening in tuberculosis.

Biochim Biophys Acta Biomembr 2019 Apr 17. Epub 2019 Apr 17.

Department of Chemistry, Indian Institute of Technology (IIT) Bombay, Mumbai, Maharashtra 400076, India. Electronic address:

Lipid structure critically dictates the molecular interactions of drugs with membranes influencing passive diffusion, drug partitioning and accumulation, thereby underpinning a lipid-composition specific interplay. Spurring selective passive drug diffusion and uptake through membranes is an obvious solution to combat growing antibiotic resistance with minimized toxicities. However, the spectrum of complex mycobacterial lipids and lack thereof of suitable membrane platforms limits the understanding of mechanisms underlying drug-membrane interactions in tuberculosis. Read More

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http://dx.doi.org/10.1016/j.bbamem.2019.04.004DOI Listing

Altered dynamics may drift pathological fibrillization in membraneless organelles.

Biochim Biophys Acta Proteins Proteom 2019 Apr 16. Epub 2019 Apr 16.

MTA-DE Laboratory of Protein Dynamics, University of Debrecen, Hungary. Electronic address:

Protein phase transition can generate non-membrane bound cellular compartments, which can convert from liquid-like to solid-like states. While the molecular driving forces of phase separation have been largely understood, much less is known about the mechanisms of material-state conversion. We apply a recently developed algorithm to describe the weak interaction network of multivalent motifs, and simulate the effect of pathological mutations. Read More

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http://dx.doi.org/10.1016/j.bbapap.2019.04.005DOI Listing

The nanoscopic molecular pathway through human skin.

Biochim Biophys Acta Gen Subj 2019 Apr 15. Epub 2019 Apr 15.

Department of Biochemistry and Molecular Biology, University of Southern Denmark, Denmark. Electronic address:

Background: Knowledge regarding the barrier properties of human skin is important for understanding skin pathology, developing of transdermal drug delivery systems and computational skin absorption models; however, the molecular pathways through human skin remains to be fully investigated on a nanoscopic level. In particular the nanoscopic pathway of molecules passing the intercellular lipid bilayers separating the corneocytes in the stratum corneum (SC) is not fully elucidated.

Methods: Using stimulated emission depletion microscopy (STED) and Förster resonance energy transfer (FRET) the molecular pathways through the SC, the main barrier of the skin, are determined for lipophilic and water-soluble molecules at a nanoscopic resolution. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S03044165193008
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http://dx.doi.org/10.1016/j.bbagen.2019.04.012DOI Listing
April 2019
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Ca signals triggered by bacterial pathogens and microdomains.

Biochim Biophys Acta Mol Cell Res 2018 Nov 18;1865(11 Pt B):1838-1845. Epub 2018 Aug 18.

Université Paris Sud, UMRS1174, Orsay F-91405, France; Institut National de la Santé et de la Recherche Médicale (Inserm), UMRS1174, Orsay F-91405, France. Electronic address:

Recent reports have highlighted the pivotal role of Ca during host cell infection by bacterial pathogens. Here, we review how bacterial pore-forming toxins (PFTs) trigger global Ca signals to regulate cell adhesion-, inflammatory- or death processes. We comment recent reports describing the role of bacterial effectors injected by a type III secretion system (T3SS) as well as host cell players in the formation of Ca microdomains during Shigella invasion and Chlamydia extrusion of host cells. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01674889183026
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http://dx.doi.org/10.1016/j.bbamcr.2018.08.007DOI Listing
November 2018
1 Read

Intracellular calcium dysregulation in autism spectrum disorder: An analysis of converging organelle signaling pathways.

Biochim Biophys Acta Mol Cell Res 2018 Nov 9;1865(11 Pt B):1718-1732. Epub 2018 Aug 9.

Department of Physiology and Biophysics, University of California, Irvine, Irvine, CA, USA; UCI Center for Autism Research and Translation, School of Medicine, University of California, Irvine, Irvine, CA, USA; Department of Pediatrics, Section of Human Genetics and Genomics, University of California, Irvine, Irvine, CA, USA. Electronic address:

Autism spectrum disorder (ASD) is a group of complex, neurological disorders that affect early cognitive, social, and verbal development. Our understanding of ASD has vastly improved with advances in genomic sequencing technology and genetic models that have identified >800 loci with variants that increase susceptibility to ASD. Although these findings have confirmed its high heritability, the underlying mechanisms by which these genes produce the ASD phenotypes have not been defined. Read More

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http://dx.doi.org/10.1016/j.bbamcr.2018.08.003DOI Listing
November 2018
1 Read

Calcium signaling and the lytic cycle of the Apicomplexan parasite Toxoplasma gondii.

Biochim Biophys Acta Mol Cell Res 2018 Nov 10;1865(11 Pt B):1846-1856. Epub 2018 Aug 10.

Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, GA 30602, USA; Department of Cellular Biology, University of Georgia, Athens, GA 30602, USA. Electronic address:

Toxoplasma gondii has a complex life cycle involving different hosts and is dependent on fast responses, as the parasite reacts to changing environmental conditions. T. gondii causes disease by lysing the host cells that it infects and it does this by reiterating its lytic cycle, which consists of host cell invasion, replication inside the host cell, and egress causing host cell lysis. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01674889183025
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http://dx.doi.org/10.1016/j.bbamcr.2018.08.004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6477927PMC
November 2018
1 Read

New insight into phase behavior and permeability of skin lipid models based on sphingosine and phytosphingosine ceramides.

Biochim Biophys Acta Biomembr 2019 Apr 13. Epub 2019 Apr 13.

Division BioTherapeutics, Leiden Academic Centre for Drug Research, Leiden University, Netherlands. Electronic address:

The intercellular lipid matrix of the stratum corneum (SC), which consist mainly of ceramides (CERs), free fatty acids and cholesterol, is fundamental to the skin barrier function. These lipids assemble into two lamellar phases, known as the long and short periodicity phases (LPP and SPP respectively). The LPP is unique in the SC and is considered important for the skin barrier function. Read More

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http://dx.doi.org/10.1016/j.bbamem.2019.04.005DOI Listing

Mechanistic approaches to understand the prion-like propagation of aggregates of the human tau protein.

Biochim Biophys Acta Proteins Proteom 2019 Apr 12. Epub 2019 Apr 12.

National Centre for Biological Sciences, Tata Institute of Fundamental Research, Bengaluru 560065, India; Indian Institute of Science Education and Research, Pune 411008, India. Electronic address:

The dynamic nature of the tau protein under physiological conditions is likely to be critical for it to perform its diverse functions inside a cell. Under some conditions, this intrinsically disordered protein assembles into pathogenic aggregates that are self-perpetuating, toxic and infectious in nature. The role of liquid-liquid phase separation in the initiation of the aggregation reaction remains to be delineated. Read More

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http://dx.doi.org/10.1016/j.bbapap.2019.04.004DOI Listing

Light-induced formation of NO in endothelial cells by photoactivatable NADPH analogues targeting nitric-oxide synthase.

Biochim Biophys Acta Gen Subj 2019 Apr 13;1863(6):1127-1137. Epub 2019 Apr 13.

LBPA, CNRS UMR8113, IDA FR3242, ENS Paris-Saclay, Université Paris-Saclay, F-94235 Cachan, France. Electronic address:

Background: Nitric-oxide synthases (NOS) catalyze the formation of NO using NADPH as electron donor. We have recently designed and synthesized a new series of two-photon absorbing and photoactivatable NADPH analogues (NT). These compounds bear one or two carboxymethyl group(s) on the 2'- or/and 3'-position(s) of the ribose in the adenosine moiety, instead of a 2'-phosphate group, and differ by the nature of the electron donor in their photoactivatable chromophore (replacing the nicotinamide moiety). Read More

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http://dx.doi.org/10.1016/j.bbagen.2019.04.004DOI Listing

The Protein Disulfide Isomerase Family: from proteostasis to pathogenesis.

Biochim Biophys Acta Gen Subj 2019 Apr 12. Epub 2019 Apr 12.

Frontier Research Institute for Interdisciplinary Sciences, Tohoku University, Aramaki aza Aoba 6-3, Aoba-ku, Sendai, Miyagi 980-8578, Japan. Electronic address:

In mammalian cells, nearly one-third of proteins are inserted into the endoplasmic reticulum (ER), where they undergo oxidative folding and chaperoning assisted by approximately 20 members of the protein disulfide isomerase family (PDIs). PDIs consist of multiple thioredoxin-like domains and recognize a wide variety of proteins via highly conserved interdomain flexibility. Although PDIs have been studied intensely for almost 50 years, exactly how they maintain protein homeostasis in the ER remains unknown, and is important not only for fundamental biological understanding but also for protein misfolding- and aggregation-related pathophysiology. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S03044165193008
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http://dx.doi.org/10.1016/j.bbagen.2019.04.003DOI Listing
April 2019
5 Reads

An insight into the dependence of the deamination rate of human APOBEC3F on the length of single-stranded DNA, which is affected by the concentrations of APOBEC3F and single-stranded DNA.

Biochim Biophys Acta Gen Subj 2019 Apr 13. Epub 2019 Apr 13.

Institute of Advanced Energy, Kyoto University, Gokasho, Uji, Kyoto, Japan; Graduate School of Energy Science, Kyoto University, Gokasho, Uji, Kyoto, Japan. Electronic address:

Background: APOBEC3F (A3F), a member of the human APOBEC3 (A3) family of cytidine deaminases, acts as an anti-HIV-1 factor by deaminating deoxycytidine in the complementary DNA of the viral genome. A full understanding of the deamination behavior of A3F awaits further investigation.

Methods: The real-time NMR method and uracil-DNA glycosylase assay were used to track the activities of the C-terminal domain (CTD) of A3F at different concentrations of A3F-CTD and ssDNA. Read More

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http://dx.doi.org/10.1016/j.bbagen.2019.04.011DOI Listing
April 2019
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Antimicrobial peptide hybrid fluorescent protein based sensor array discriminate ten most frequent clinic isolates.

Biochim Biophys Acta Gen Subj 2019 Apr 12;1863(6):1158-1166. Epub 2019 Apr 12.

Center for Clinical Laboratory Medicine of Zhongda Hospital, Southeast University, China. Electronic address:

Antimicrobial peptides killed bacteria through intercalating into the bacterial membrane. Their antimicrobial efficiencies varied in bacterial species and were affected by ion strength in the culture medium. A recombinant IGP protein consisted of an antimicrobial peptide, Ib-AMP4 fused with the Green Fluorescent Protein was expressed from E. Read More

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http://dx.doi.org/10.1016/j.bbagen.2019.04.010DOI Listing
April 2019
4.381 Impact Factor

Mechanism and impact of catecholamine conversion by Vibrio cholerae.

Biochim Biophys Acta Bioenerg 2019 Apr 12. Epub 2019 Apr 12.

Institute of Microbiology, University of Hohenheim, Stuttgart, Germany. Electronic address:

Bacterial pathogens are influenced by signaling molecules including the catecholamines adrenaline and noradrenaline which are host-derived hormones and neurotransmitters. Adrenaline and noradrenaline modulate growth, motility and virulence of bacteria. We show that adrenaline is converted by the pathogen Vibrio cholerae to adrenochrome in the course of respiration, and demonstrate that superoxide produced by the respiratory, Na - translocating NADH:quinone oxidoreductase (NQR) acts as electron acceptor in the oxidative conversion of adrenaline to adrenochrome. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00052728193003
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http://dx.doi.org/10.1016/j.bbabio.2019.04.003DOI Listing
April 2019
2 Reads

Fourier transform visible and infrared difference spectroscopy for the study of P700 in photosystem I from Fischerella thermalis PCC 7521 cells grown under white light and far-red light: Evidence that the A cofactor is chlorophyll f.

Biochim Biophys Acta Bioenerg 2019 Apr 13;1860(6):452-460. Epub 2019 Apr 13.

Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA, USA; Department of Chemistry and Biochemistry, Montana State University, Bozeman, MT, USA.

(P700 - P700) Fourier transform visible and infrared difference spectra (DS) have been obtained using photosystem I (PSI) complexes isolated from cells of Fischerella thermalis PCC 7521 grown under white light (WL) or far-red light (FRL). PSI from cells grown under FRL (FRL-PSI) contain ~8 chlorophyll f (Chl f) molecules (Shen et al., Photosynth. Read More

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http://dx.doi.org/10.1016/j.bbabio.2019.04.002DOI Listing

Ultrashort nanosecond electric pulses evoke heterogeneous patterns of Ca release from the endoplasmic reticulum of adrenal chromaffin cells.

Biochim Biophys Acta Biomembr 2019 Apr 12;1861(6):1180-1188. Epub 2019 Apr 12.

Department of Pharmacology, University of Nevada, Reno School of Medicine, Reno, NV 89557, USA.

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http://dx.doi.org/10.1016/j.bbamem.2019.04.006DOI Listing

Calcium, mitochondria and cell metabolism: A functional triangle in bioenergetics.

Biochim Biophys Acta Mol Cell Res 2019 Jul 26;1866(7):1068-1078. Epub 2018 Oct 26.

Department of Biomedical Sciences, University of Padova, Via U. Bassi 58/B, 35131 Padova, Italy.

The versatility of mitochondrial metabolism and its fine adjustments to specific physiological or pathological conditions regulate fundamental cell pathways, ranging from proliferation to apoptosis. In particular, Ca signalling has emerged as a key player exploited by mitochondria to tune their activity according with cell demand. The functional interaction between mitochondria and endoplasmic reticulum (ER) deeply impacts on the correct mitochondrial Ca signal, thus modulating cell bioenergetics and functionality. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01674889183047
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http://dx.doi.org/10.1016/j.bbamcr.2018.10.016DOI Listing
July 2019
1 Read

Tumor protein D52 (isoform 3) interacts with and promotes peroxidase activity of Peroxiredoxin 1 in prostate cancer cells implicated in cell growth and migration.

Biochim Biophys Acta Mol Cell Res 2019 Apr 11. Epub 2019 Apr 11.

Department of Applied Biology, CSIR-Indian Institute of Chemical Technology (CSIR-IICT), Hyderabad, India; Academy of Scientific & Innovative Research (AcSIR), CSIR-Indian Institute of Chemical Technology (CSIR-IICT), Hyderabad, India. Electronic address:

Tumor protein D52 (TPD52) is overexpressed in multiple cancers including prostate cancer due to gene amplification and investigations to understand its role in the pathophysiology of different cancers are continuing. GST pull-down assays and Tandem affinity purification of TPD52 as bait identified novel prey Peroxiredoxin 1 (PRDX1) in prostate cancer (PCa) cells. PRDX1 interaction with TPD52 was confirmed in immunoprecipitation and affinity interaction assays. Read More

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http://dx.doi.org/10.1016/j.bbamcr.2019.04.007DOI Listing

The metastasis suppressor, NDRG1, differentially modulates the ER stress response in the presence and absence of the anti-cancer agent, Dp44mT.

Biochim Biophys Acta Mol Basis Dis 2019 Apr 11. Epub 2019 Apr 11.

Molecular Pharmacology and Pathology Program, Discipline of Pathology and Bosch Institute, Medical Foundation Building (K25), The University of Sydney, Sydney, New South Wales 2006, Australia; Department of Pathology and Biological Responses, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa-ku, Nagoya 466-8550, Japan. Electronic address:

The metastasis suppressor, N-myc downstream regulated gene-1 (NDRG1), is a stress response protein that is involved in the inhibition of multiple oncogenic signaling pathways. Initial studies have linked NDRG1 and the endoplasmic reticulum (ER) stress response. Considering this, we extensively examined the mechanism by which NDRG1 regulates the ER stress response in pancreatic and colon cancer cells. Read More

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http://dx.doi.org/10.1016/j.bbadis.2019.04.007DOI Listing

The power of small changes: Comprehensive analyses of microbial dysbiosis in breast cancer.

Biochim Biophys Acta Rev Cancer 2019 Apr 11;1871(2):392-405. Epub 2019 Apr 11.

Department of Oncology, Johns Hopkins University School of Medicine and the Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins, Baltimore, MD, USA. Electronic address:

Disparate occurrence of breast cancer remains an intriguing question since only a subset of women with known risk factors develop cancer. Recent studies suggest an active role of local and distant microbiota in breast cancer initiation, progression, and overall prognosis. A dysbiotic microbiota predisposes the body to develop cancer by inducing genetic instability, initiating DNA damage and proliferation of the damaged progeny, eliciting favorable immune response, metabolic dysregulation and altered response to therapy. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S0304419X193004
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http://dx.doi.org/10.1016/j.bbcan.2019.04.001DOI Listing
April 2019
1 Read

Yeast Dop1 is required for glycosyltransferase retrieval from the trans-Golgi network.

Biochim Biophys Acta Gen Subj 2019 Apr 11;1863(6):1147-1157. Epub 2019 Apr 11.

Key Laboratory of Carbohydrate Chemistry, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, Jiangsu 214122, China. Electronic address:

Background: Glycosyltransferases are type II membrane proteins that are responsible for glycan modification of proteins and lipids, and localize to distinct cisternae in the Golgi apparatus. During cisternal maturation, retrograde trafficking helps maintain the steady-state localization of these enzymes in the sub-compartments of the Golgi.

Methods: To understand how glycosyltransferases are recycled in the late Golgi complex, we searched for genes that are essential for budding yeast cell growth and that encode proteins localized in endosomes and in the Golgi. Read More

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http://dx.doi.org/10.1016/j.bbagen.2019.04.009DOI Listing
April 2019
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Metabolic activation triggered by cAMP in MCF-7 cells generates lethal vulnerability to combined oxamate/etomoxir.

Biochim Biophys Acta Gen Subj 2019 Apr 11. Epub 2019 Apr 11.

Department of Experimental, Diagnostic and Specialty Medicine (DIMES), University of Bologna, Italy. Electronic address:

Background: Altered energy metabolism is a biochemical fingerprint of cancer cells, widely recognized as one of the "hallmarks of cancer". Cancer cells show highly increased rates of glucose uptake and glycolysis, after which the resulting pyruvate is converted to lactate. The maintenance of this metabolic asset is warranted by lactate dehydrogenase A (LDH-A) and for this reason the development of novel LDH-targeted anticancer therapeutics is underway. Read More

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http://dx.doi.org/10.1016/j.bbagen.2019.04.008DOI Listing

Modulation of biological responses to 2 ns electrical stimuli by field reversal.

Biochim Biophys Acta Biomembr 2019 Apr 11. Epub 2019 Apr 11.

Frank Reidy Research Center for Bioelectrics, Old Dominion University, 4211 Monarch Way Suite 300, Norfolk, VA 23508, USA.

Nanosecond bipolar pulse cancellation, a recently discovered phenomenon, is modulation of the effects of a unipolar electric pulse exposure by a second pulse of opposite polarity. This attenuation of biological response by reversal of the electric field direction has been reported with pulse durations from 60 ns to 900 ns for a wide range of endpoints, and it is not observed with conventional electroporation pulses of much longer duration (>100 μs) where pulses are additive regardless of polarity. The most plausible proposed mechanisms involve the field-driven migration of ions to and from the membrane interface (accelerated membrane discharge). Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00052736193007
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http://dx.doi.org/10.1016/j.bbamem.2019.03.019DOI Listing
April 2019
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Lipid mediators in platelet concentrate and extracellular vesicles: Molecular mechanisms from membrane glycerophospholipids to bioactive molecules.

Biochim Biophys Acta Mol Cell Biol Lipids 2019 Apr 10. Epub 2019 Apr 10.

Finnish Red Cross Blood Service, Kivihaantie 7, 00310 Helsinki, Finland. Electronic address:

Platelets are collected for transfusion to patients with different hematological disorders, and for logistical reasons, platelets are stored as concentrates. Despite the carefully controlled conditions, platelets become activated during storage, and platelet concentrates (PLCs) may cause adverse inflammatory reactions in the recipients. We studied by mass spectrometry the lipidomic changes during storage of the clinical PLCs, the platelets isolated from PLCs, and the extracellular vesicles (EVs) thereof. Read More

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http://dx.doi.org/10.1016/j.bbalip.2019.03.011DOI Listing

Myristic acid specifically stabilizes diacylglycerol kinase δ protein in C2C12 skeletal muscle cells.

Biochim Biophys Acta Mol Cell Biol Lipids 2019 Jul 11;1864(7):1031-1038. Epub 2019 Apr 11.

Department of Chemistry, Graduate School of Science, Chiba University, Chiba 263-8522, Japan. Electronic address:

Decreased levels of the δ isozyme of diacylglycerol kinase (DGK) in skeletal muscle attenuate glucose uptake and, consequently, are critical for the pathogenesis of type 2 diabetes. We recently found that free myristic acid (14:0), but not free palmitic acid (16:0), increased the DGKδ protein levels and enhanced glucose uptake in C2C12 myotube cells. However, it has been unclear how myristic acid regulates the level of DGKδ2 protein. Read More

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http://dx.doi.org/10.1016/j.bbalip.2019.04.003DOI Listing
July 2019
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Tripeptide IRW initiates differentiation in osteoblasts differentiation via the RUNX2 pathway.

Biochim Biophys Acta Gen Subj 2019 Apr 10;1863(6):1138-1146. Epub 2019 Apr 10.

Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB, Canada. Electronic address:

Background: Osteoblasts maintain the structural integrity of bone via differentiation and mineralization; therefore, their malfunction or reduced activity can cause serious bone disorders. Although studies have demonstrated the association between nutrients and bone, research on food-derived bioactive peptides and bone health are scanty.

Methods: Osteoblasts MC3T3-E1 were treated with IRW (50 and 25 μM). Read More

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http://dx.doi.org/10.1016/j.bbagen.2019.04.007DOI Listing
April 2019
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Dietary cholesterol is essential to mast cell activation and associated obesity and diabetes in mice.

Biochim Biophys Acta Mol Basis Dis 2019 Apr 9. Epub 2019 Apr 9.

Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA. Electronic address:

Mast cell (MC) deficiency in Kit mice and inhibition with disodium chromoglycate (DSCG) or ketotifen reduced obesity and diabetes in mice on a high-cholesterol (1.25%) Western diet. Yet, Kit-independent MC-deficient mice and mice treated with DSCG disproved MC function in obesity and diabetes when mice are fed a high-fat diet (HFD) that contains no cholesterol. Read More

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http://dx.doi.org/10.1016/j.bbadis.2019.04.006DOI Listing
April 2019
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4.882 Impact Factor

Atomistic modeling and molecular dynamics analysis of human Ca1.2 channel using external electric field and ion pulling simulations.

Biochim Biophys Acta Gen Subj 2019 Apr 9;1863(6):1116-1126. Epub 2019 Apr 9.

Faculty Of Pharmacy And Pharmaceutical Sciences, University Of Alberta, Edmonton, Ab, Canada; Li Ka Shing Institute Of Virology, University Of Alberta, Edmonton, Alberta, Canada; Li Ka Shing Applied Virology Institute, University Of Alberta, Edmonton, Alberta, Canada. Electronic address:

Background: Human Ca1.2 (hCav1.2), a calcium selective voltage-gated channel, plays important roles in normal cardiac and neuronal functions. Read More

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http://dx.doi.org/10.1016/j.bbagen.2019.04.006DOI Listing
April 2019
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A biophysical study of the interactions between the antimicrobial peptide indolicidin and lipid model systems.

Biochim Biophys Acta Biomembr 2019 Apr 9. Epub 2019 Apr 9.

Department of Chemistry, University of Oslo, 0315 Oslo, Norway. Electronic address:

The naturally occurring peptide indolicidin from bovine neutrophils exhibits strong biological activity against a broad spectrum of microorganisms. This is believed to arise from selective interactions with the negatively charged cytoplasmic lipid membrane found in bacteria. We have investigated the peptide interaction with supported lipid model membranes using a combination of complementary surface sensitive techniques: neutron reflectometry (NR), atomic force microscopy (AFM), and quartz crystal microbalance with dissipation monitoring (QCM-D). Read More

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http://dx.doi.org/10.1016/j.bbamem.2019.04.003DOI Listing
April 2019
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An SOD1 deficiency aggravates proteasome inhibitor bortezomib-induced testicular damage in mice.

Biochim Biophys Acta Gen Subj 2019 Apr 8;1863(6):1108-1115. Epub 2019 Apr 8.

Department of Biochemistry and Molecular Biology, Graduate School of Medical Science, Yamagata University, 2-2-2 Iidanishi, Yamagata 990-9585, Japan.

Proteasomes play a key role in maintaining cellular homeostasis by the proteolytic removal of proteins, including ubiquitinated proteins and/or oxidatively-damaged proteins. The proteasome inhibitor bortezomib (BTZ) has been reported to exert testicular toxicity in mice. In the current study, we treated SOD1-knockout (KO) mice with BTZ and investigated the issue of whether oxidative stress is involved in the development of testicular toxicity. Read More

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http://dx.doi.org/10.1016/j.bbagen.2019.04.005DOI Listing
April 2019
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Sub-inhibitory membrane damage undermines Staphylococcus aureus virulence.

Biochim Biophys Acta Biomembr 2019 Apr 8;1861(6):1172-1179. Epub 2019 Apr 8.

Department of Biotechnology and Food Engineering, Technion-Israel Institute of Technology, Haifa, Israel. Electronic address:

We investigated antibacterial properties of a recently described membrane-active lipopeptide, COOcO (decanoyl-ornithyl-ornithyl-dodecanoyl-ornithyl-amide) against Gram-positive bacteria (GPB). Minimal inhibitory concentrations (MICs) and kinetics were compared in culture media and plasma. Chemo-sensitization to antibiotics was determined using the checkerboard assay. Read More

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http://dx.doi.org/10.1016/j.bbamem.2019.04.002DOI Listing
April 2019
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Phospholipid distributions in purple phototrophic bacteria and LH1-RC core complexes.

Biochim Biophys Acta Bioenerg 2019 Apr 8. Epub 2019 Apr 8.

Faculty of Science, Ibaraki University, Mito 310-8512, Japan. Electronic address:

In contrast to plants, algae and cyanobacteria that contain glycolipids as the major lipid components in their photosynthetic membranes, phospholipids are the dominant lipids in the membranes of anoxygenic purple phototrophic bacteria. Although the phospholipid compositions in whole cells or membranes are known for a limited number of the purple bacteria, little is known about the phospholipids associated with individual photosynthetic complexes. In this study, we investigated the phospholipid distributions in both membranes and the light-harvesting 1-reaction center (LH1-RC) complexes purified from several purple sulfur and nonsulfur bacteria. Read More

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http://dx.doi.org/10.1016/j.bbabio.2019.04.001DOI Listing

The intracellular NPxY motif is critical in maintaining the function and expression of human organic anion transporting polypeptide 1B1.

Biochim Biophys Acta Biomembr 2019 Apr 7;1861(6):1189-1196. Epub 2019 Apr 7.

College of Life Sciences, South China Agricultural University, Guangzhou, China; Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, South China Agricultural University, Guangzhou, China. Electronic address:

Organic anion transporting polypeptides (OATPs, gene symbol SLCO) mediate sodium-independent transport of endogenous compounds such as bile salts, hormones and their conjugates as well as toxins and drugs. OATP1B1 is the major OATP specifically expressed at the basolateral membrane of human hepatocytes and many clinically important drugs have been shown to be substrates of the transporter. According to the computer-based hydropathy analysis, a large intracellular loop 3 (IL3) is situated between transmembrane domain 6 and 7 of OATPs, in which a conserved NPxY motif is found. Read More

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http://dx.doi.org/10.1016/j.bbamem.2019.04.001DOI Listing

Analysis of the pathogenic I326T variant of human tRNA nucleotidyltransferase reveals reduced catalytic activity and thermal stability in vitro linked to a conformational change.

Biochim Biophys Acta Proteins Proteom 2019 Apr 5;1867(6):616-626. Epub 2019 Apr 5.

Department of Chemistry and Biochemistry and Centre for Structural and Functional Genomics, Concordia University, 7141 Sherbrooke St. W., Montréal H4B 1R6, Québec, Canada. Electronic address:

The I326T mutation in the TRNT1 gene encoding human tRNA nucleotidyltransferase (tRNA-NT) is linked to a relatively mild form of SIFD. Previous work indicated that the I326T variant was unable to incorporate AMP into tRNAs in vitro, however, expression of the mutant allele from a strong heterologous promoter supported in vivo CCA addition to both cytosolic and mitochondrial tRNAs in a yeast strain lacking tRNA-NT. To address this discrepancy, we determined the biochemical and biophysical characteristics of the I326T variant enzyme and the related variant, I326A. Read More

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http://dx.doi.org/10.1016/j.bbapap.2019.04.003DOI Listing

Zbtb7c is a critical gluconeogenic transcription factor that induces glucose-6-phosphatase and phosphoenylpyruvate carboxykinase 1 genes expression during mice fasting.

Biochim Biophys Acta Gene Regul Mech 2019 Apr 5. Epub 2019 Apr 5.

BK21 plus project of Medical Science, Department of Biochemistry and Molecular Biology, Yonsei University School of Medicine, 50-1 Yonsei-Ro, SeoDaeMoon-Ku, Seoul 03722, Republic of Korea. Electronic address:

Gluconeogenesis is essential for blood glucose homeostasis during fasting and is regulated by various enzymes, which are encoded by gluconeogenic genes. Those genes are controlled by various transcription factors. Zinc finger and BTB domain-containing 7c (Zbtb7c, also called Kr-pok) is a BTB-POZ family transcription factor with proto-oncogenic activity. Read More

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http://dx.doi.org/10.1016/j.bbagrm.2019.04.001DOI Listing
April 2019
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6.332 Impact Factor

Substrate channeling in the glycerol-3-phosphate pathway regulates the synthesis, storage and secretion of glycerolipids.

Biochim Biophys Acta Mol Cell Biol Lipids 2019 Apr 5. Epub 2019 Apr 5.

Atlantic Research Center, Depts. of Pediatrics and Biochemistry & Molecular Biology, Dalhousie University, Halifax, NS, Canada. Electronic address:

The successive acylation of glycerol-3-phosphate (G3P) by glycerol-3-phosphate acyltransferases and acylglycerol-3-phosphate acyltransferases produces phosphatidic acid (PA), a precursor for CDP-diacylglycerol-dependent phospholipid synthesis. PA is further dephosphorylated by LIPINs to produce diacylglycerol (DG), a substrate for the synthesis of triglyceride (TG) by DG acyltransferases and a precursor for phospholipid synthesis via the CDP-choline and CDP-ethanolamine (Kennedy) pathways. The channeling of fatty acids into TG for storage in lipid droplets and secretion in lipoproteins or phospholipids for membrane biogenesis is dependent on isoform expression, activity and localization of G3P pathway enzymes, as well as dietary and hormonal and tissue-specific factors. Read More

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http://dx.doi.org/10.1016/j.bbalip.2019.03.010DOI Listing

P53/NRF2 mediates SIRT1's protective effect on diabetic nephropathy.

Biochim Biophys Acta Mol Cell Res 2019 Apr 6;1866(8):1272-1281. Epub 2019 Apr 6.

Department of Toxicology and Nutrition, School of Public Health, Shandong University, 44 Wenhua Xi Rd., Jinan, Shandong 250012, China. Electronic address:

Diabetic nephropathy (DN) is the leading cause of end stage renal disease, posing a severe threat to public health. Previous studies reported the protective role of sirtuin 1 (SIRT1) in DN, encouraging the investigation of more potent and specific SIRT1 activators. SRT2104 is a novel, first-in-class, highly selective small-molecule activator of SIRT1, with its effect and mechanism unknown on DN. Read More

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http://dx.doi.org/10.1016/j.bbamcr.2019.04.006DOI Listing
April 2019
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