4,104 results match your criteria Biochimica et Biophysica Acta[Journal]


Quantitative Determination of 18--Glycyrrhetinic Acid in HepG2 Cell Line by High Performance Liquid Chromatography Method.

Int J Anal Chem 2018 13;2018:5673186. Epub 2018 Nov 13.

UOC Odontoiatria Generale e Ortodonzia, Dip Scienze dell'Invecchiamento, Neurologiche, Ortopediche e della Testa Collo. Fondazione Policlinico Universitario A. Gemelli, IRCCS, Università Cattolica del Sacro Cuore Rome, Italy.

A reverse phase high performance liquid chromatographic (RP-HPLC) method was developed for identification and estimation of 18--glycyrrhetinic acid (GA) in HepG2 cell line. The analysis was carried out using a JASCO HPLC system with a C-18 (3 m) Supelco reversed phase column (150 x 4.7 mm) using a mobile phase of 80% CHOH and 20% of CHCN: tetrahydrofuran: water (10:80:10, v/v/v). Read More

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November 2018
2 Reads

Utility of peripheral blood smear in rapid diagnosis of Pertussis.

Int J Lab Hematol 2018 Nov 27. Epub 2018 Nov 27.

A.O.R.N. "G. Rummo" Benevento, U.O. Patologia Clinica, Benevento, Italy.

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November 2018

Dietary phytochemicals in colorectal cancer prevention and treatment: A focus on the molecular mechanisms involved.

Biotechnol Adv 2018 Nov 23. Epub 2018 Nov 23.

Nutrition and Food Science Group, Dept. of Analytical and Food Chemistry, CITACA, CACTI, University of Vigo, Vigo Campus, Vigo, (Spain); Dipartimento di Scienze Cliniche Specialistiche ed Odontostomatologiche (DISCO)-Sez. Biochimica, Facoltà di Medicina, Università Politecnica delle Marche, Ancona 60131, Italy. Electronic address:

Worldwide, colorectal cancer (CRC) remains a major cancer type and leading cause of death. Unfortunately, current medical treatments are not sufficient due to lack of effective therapy, adverse side effects, chemoresistance and disease recurrence. In recent decades, epidemiologic observations have highlighted the association between the ingestion of several phytochemical-enriched foods and nutrients and the lower risk of CRC. Read More

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November 2018
1 Read

Molecular and Cellular Functions of the Warsaw Breakage Syndrome DNA Helicase DDX11.

Genes (Basel) 2018 Nov 21;9(11). Epub 2018 Nov 21.

Elettra⁻Sincrotrone Trieste S.C.p.A., AREA Science Park Basovizza, 34149 Trieste, Italy.

DDX11/ChlR1 (Chl1 in yeast) is a DNA helicase involved in sister chromatid cohesion and in DNA repair pathways. The protein belongs to the family of the iron⁻sulphur cluster containing DNA helicases, whose deficiencies have been linked to a number of diseases affecting genome stability. Mutations of human DDX11 are indeed associated with the rare genetic disorder named Warsaw breakage syndrome, showing both chromosomal breakages and chromatid cohesion defects. Read More

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November 2018
1 Read

The antioxidant power of horseradish, Armoracia rusticana, underlies antimicrobial and antiradical effects, exerted in vitro.

Nat Prod Res 2018 Nov 21:1-4. Epub 2018 Nov 21.

b Institute for Coastal Marine Environment (IAMC), National Council Research (CNR) , Messina , Italy.

Armoracia rusticana (AR) was tested for antimicrobial and antioxidants power. The compound demonstrated to inhibit fish pathogens such as Vibrio anguillarum, V. harvey, V. Read More

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November 2018
2 Reads

Maria Teresa Carrì Obituary.

Authors:
Giuseppe Rotilio

Cell Death Dis 2018 Nov 19;9(12):1153. Epub 2018 Nov 19.

University of Tor Vergata, Dept Biochimica, Rome, Italy.

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November 2018
2 Reads

Fibrosis rescue improves cardiac function in dystrophin-deficient mice and Duchenne patient-specific cardiomyocytes by immunoproteasome modulation.

Am J Pathol 2018 Nov 15. Epub 2018 Nov 15.

Stem Cell Laboratory, Department of Pathophysiology and Transplantation, Universitá degli Studi di Milano, Unit of Neurology, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, Centro Dino Ferrari, Milan, Italy. Electronic address:

Patients affected by Duchenne muscular dystrophy (DMD) develop a progressive dilated cardiomyopathy characterized by inflammatory cell infiltration, necrosis, and cardiac fibrosis. Standard treatments consider the use of β-blockers and angiotensin-converting enzyme inhibitors that are symptomatic and unspecific towards DMD disease. Medications that target DMD cardiac fibrosis are in early stages of development. Read More

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November 2018
3 Reads

A new case report of severe mucopolysaccharidosis type VII: diagnosis, treatment with haematopoietic cell transplantation and prenatal diagnosis in a second pregnancy.

Ital J Pediatr 2018 Nov 16;44(Suppl 2):128. Epub 2018 Nov 16.

Clinica Pediatrica, Fondazione MBBM, Università Milano-Bicocca, Monza, Italy.

A new patient with severe mucopolysaccharidosis (MPS) type VII is reported. Non-immune hydrops fetalis (NIHF) was diagnosed during pregnancy. At birth, he showed generalized hydrops and dysmorphic features typical of MPS. Read More

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November 2018
5 Reads
1.240 Impact Factor

Emerging Role of Purine Metabolizing Enzymes in Brain Function and Tumors.

Int J Mol Sci 2018 Nov 14;19(11). Epub 2018 Nov 14.

Dipartimento di Biologia, Unità di Biochimica, Via San Zeno 51, 56127 Pisa, Italy.

The growing evidence of the involvement of purine compounds in signaling, of nucleotide imbalance in tumorigenesis, the discovery of purinosome and its regulation, cast new light on purine metabolism, indicating that well known biochemical pathways may still surprise. Adenosine deaminase is important not only to preserve functionality of immune system but also to ensure a correct development and function of central nervous system, probably because its activity regulates the extracellular concentration of adenosine and therefore its function in brain. A lot of work has been done on extracellular 5'-nucleotidase and its involvement in the purinergic signaling, but also intracellular nucleotidases, which regulate the purine nucleotide homeostasis, play unexpected roles, not only in tumorigenesis but also in brain function. Read More

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November 2018
2 Reads

Prospective diagnostic and prognostic study of copeptin in suspected acute aortic syndromes.

Sci Rep 2018 Nov 13;8(1):16713. Epub 2018 Nov 13.

S.C.U. Medicina d'Urgenza, A.O.U. Città della Salute e della Scienza, Molinette Hospital, Torino, Italy.

Acute aortic syndromes (AAS) are cardiovascular emergencies with unmet diagnostic needs. Copeptin is released upon stress conditions and is approved for rule-out of myocardial infarction (MI). As MI and AAS share presenting symptoms, stress mechanisms and necessity for rapid diagnosis, copeptin appears as an attractive biomarker also for AAS. Read More

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November 2018
1 Read

Changes in renal papillary density after hydration therapy in calcium stone formers.

BMC Urol 2018 Nov 12;18(1):101. Epub 2018 Nov 12.

U.O.C. Nefrologia, Fondazione Policlinico Universitario A. Gemelli IRCCS, Roma, Italia.

Background: Previous studies have shown that, compared with non-stone formers, stone formers have a higher papillary density measured with computer tomography (CT) scan. The effect of increased hydration on such papillary density in idiopathic calcium stone formers is not known.

Methods: Patients with recurrent calcium oxalate stones undergoing endourological procedures for renal stones at our Institution from June 2013 to June 2014 were considered eligible for enrolment. Read More

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November 2018
2 Reads

The Neuroprotective Effects of 17β-Estradiol Pretreatment in a Model of Neonatal Hippocampal Injury Induced by Trimethyltin.

Front Cell Neurosci 2018 26;12:385. Epub 2018 Oct 26.

Institute of Anatomy and Cell Biology, Università Cattolica del Sacro Cuore, Rome, Italy.

Hippocampal dysfunction plays a central role in neurodevelopmental disorders, resulting in severe impairment of cognitive abilities, including memory and learning. On this basis, developmental studies represent an important tool both to understanding the cellular and molecular phenomena underlying early hippocampal damage and to study possible therapeutic interventions, that may modify the progression of neuronal death. Given the modulatory role played by 17β-estradiol (E2) on hippocampal functions and its neuroprotective properties, the present study investigates the effects of pretreatment with E2 in a model of neonatal hippocampal injury obtained by trimethyltin (TMT) administration, characterized by neuronal loss in CA1 and CA3 subfields and astroglial and microglial activation. Read More

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October 2018
3 Reads

G protein subunit phosphorylation as a regulatory mechanism in heterotrimeric G protein signaling in mammals, yeast, and plants.

Biochem J 2018 Nov 9;475(21):3331-3357. Epub 2018 Nov 9.

Biology Department, Penn State University, 208 Mueller Laboratory, University Park, PA 16802, U.S.A.

Heterotrimeric G proteins composed of Gα, Gβ, and Gγ subunits are vital eukaryotic signaling elements that convey information from ligand-regulated G protein-coupled receptors (GPCRs) to cellular effectors. Heterotrimeric G protein-based signaling pathways are fundamental to human health [ (2007) , 994-1005] and are the target of >30% of pharmaceuticals in clinical use [ (2013) , 1676-1694; (2017) , 829-842]. This review focuses on phosphorylation of G protein subunits as a regulatory mechanism in mammals, budding yeast, and plants. Read More

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November 2018
2 Reads

Recurrent kidney stones in a family with a mitochondrial disorder due to the m.3243A>G mutation.

Urolithiasis 2018 Nov 7. Epub 2018 Nov 7.

U.O.C. Nefrologia, Fondazione Policlinico Universitario A. Gemelli IRCCS, Roma, Italia.

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November 2018

Lipid exchange and signaling at ER-Golgi contact sites.

Curr Opin Cell Biol 2018 Oct 31;57:8-15. Epub 2018 Oct 31.

Interfaculty Institute of Bioengineering, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland; Istituto di Biochimica delle Proteine, CNR, Naples, Italy. Electronic address:

Meticulous observations of the cell perinuclear region where the Golgi and the endoplasmic reticulum (ER) networks intermingle have revealed close contact sites of barely 20 nm between these two organelles. Recent studies demonstrate that molecular machineries, including lipid-transfer proteins, enriched in membrane contact sites between ER and trans-Golgi are capable of bridging membranes and exchanging key lipids such as sphingolipid precursors and cholesterol while bypassing the early secretory compartments. This occurs at the cost of an intense phosphoinositide turnover in order to prepare a membrane environment conducive to the signaling and trafficking functions of the trans-Golgi network. Read More

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October 2018
4 Reads

Effects of phytochemicals on thyroid function and their possible role in thyroid disease.

Ann N Y Acad Sci 2018 Nov 1. Epub 2018 Nov 1.

Dipartimento di Scienze Cliniche Specialistiche ed Odontostomatologiche, Sez, Biochimica, Università Politecnica delle Marche, Ancona, Italy.

About 1 of 10 women, particularly those older than 60 years of age, shows some degree of thyroid hormone deficiency. Thyroid diseases are generally characterized by perturbations of thyroid signaling homeostasis. The most common examples of thyroid diseases include hypothyroidism, hyperthyroidism, and several types of thyroid cancers. Read More

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November 2018
6 Reads

The extreme hyper-reactivity of Cys94 in lysozyme avoids its amorphous aggregation.

Sci Rep 2018 Oct 30;8(1):16050. Epub 2018 Oct 30.

Department of Chemical Sciences and Technologies, University of Rome "Tor Vergata", Rome, Italy.

Many proteins provided with disulfide bridges in the native state undergo amorphous irreversible aggregation when these bonds are not formed. Here we show that egg lysozyme displays a clever strategy to prevent this deleterious aggregation during the nascent phase when disulfides are still absent. In fact, when the reduced protein assembles into a molten globule state, its cysteines acquire strong hyper-reactivity towards natural disulfides. Read More

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October 2018
4 Reads

Validation of UPLC-MS/MS Method for Determination of Urinary Lactulose/Mannitol.

Molecules 2018 Oct 20;23(10). Epub 2018 Oct 20.

Fondazione Policlinico Universitario A. Gemelli IRCCS, 00168 Roma, Italy.

Determination of urinary lactulose/mannitol is one of the most used tests to evaluate intestinal barrier function. High-performance liquid chromatography (HPLC) separation with electrospray ionization tandem mass spectrometry guarantees high levels of selectivity and reproducibility. In this paper we report an upgrade of the previous published liquid chromatography tandem mass spectrometry method, introducing more reliable internal standards and ultra-performance liquid chromatography with ethylene bridged hybrid amide columns. Read More

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October 2018

Protein nitration profile of CD3 lymphocytes from Alzheimer disease patients: Novel hints on immunosenescence and biomarker detection.

Free Radic Biol Med 2018 Dec 12;129:430-439. Epub 2018 Oct 12.

Department of Biomedical Sciences and Biotechnologies, University of Brescia, Brescia, Italy.

Alzheimer's disease (AD) is a progressive form of dementia characterized by increased production of amyloid-β plaques and hyperphosphorylated tau protein, mitochondrial dysfunction, elevated oxidative stress, reduced protein clearance, among other. Several studies showed systemic modifications of immune and inflammatory systems due, in part, to decreased levels of CD3 lymphocytes in peripheral blood in AD. Considering that oxidative stress, both in the brain and in the periphery, can influence the activation and differentiation of T-cells, we investigated the 3-nitrotyrosine (3-NT) proteome of blood T-cells derived from AD patients compared to non-demented (ND) subjects by using a proteomic approach. Read More

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December 2018
4 Reads

Interaction of the Warsaw breakage syndrome DNA helicase DDX11 with the replication fork-protection factor Timeless promotes sister chromatid cohesion.

PLoS Genet 2018 Oct 10;14(10):e1007622. Epub 2018 Oct 10.

Istituto di Biochimica delle Proteine, Consiglio Nazionale Ricerche, Naples, Italy.

Establishment of sister chromatid cohesion is coupled to DNA replication, but the underlying molecular mechanisms are incompletely understood. DDX11 (also named ChlR1) is a super-family 2 Fe-S cluster-containing DNA helicase implicated in Warsaw breakage syndrome (WABS). Herein, we examined the role of DDX11 in cohesion establishment in human cells. Read More

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October 2018
1 Read

Proteasome-mediated remodeling of the proteome and phosphoproteome during kiwifruit pollen germination.

J Proteomics 2018 Sep 27. Epub 2018 Sep 27.

Dipartimento di Scienze Biomolecolari, Sezione di Biochimica e Biologia Molecolare, Università degli Studi di Urbino "Carlo Bo", via Saffi 2, Urbino 61029, Italy. Electronic address:

Proteasome activity is essential for pollen tube emergence and growth; nevertheless, little is known about proteasome function at the molecular level. The objective of this study was to identify molecular targets and pathways which are directly/indirectly controlled by the proteasome during pollen germination. To this aim, changes in the proteome and phosphoproteome of Actinidia pollen, germinated in the presence of the proteasome inhibitor MG132, were investigated. Read More

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September 2018
9 Reads

Broad phenotypic spectrum and genotype-phenotype correlations in GMPPB-related dystroglycanopathies: an Italian cross-sectional study.

Orphanet J Rare Dis 2018 Sep 26;13(1):170. Epub 2018 Sep 26.

Department of Developmental Neuroscience and Molecular Medicine Neuromuscular Unit and Child Neurology, IRCCS Fondazione Stella Maris, Via dei Giacinti 2, 56018, Pisa, Italy.

Background: Dystroglycanopathy (α-DG) is a relatively common, clinically and genetically heterogeneous category of congenital forms of muscular dystrophy (CMD) and limb-girdle muscular dystrophy (LGMD) associated with hypoglycosylated α-dystroglycan. To date, mutations in at least 19 genes have been associated with α-DG. One of them, GMPPB, encoding the guanosine-diphosphate-mannose (GDP-mannose) pyrophosphorylase B protein, has recently been associated with a wide clinical spectrum ranging from severe Walker-Warburg syndrome to pseudo-metabolic myopathy and even congenital myasthenic syndromes. Read More

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September 2018
5 Reads

Author Correction: MYC-driven epigenetic reprogramming favors the onset of tumorigenesis by inducing a stem cell-like state.

Nat Commun 2018 09 20;9(1):3921. Epub 2018 Sep 20.

Laboratory of Chromatin Biology & Epigenetics, Center for Integrative Biology (CIBIO), University of Trento, 38123, Trento, Italy.

The original version of this Article contained an error in the spelling of the author Miriam Gaggianesi, which was incorrectly given as Miriam Giaggianesi. Furthermore, the affiliation details for Gabriella Gaudioso, Valentina Vaira, and Silvano Bosari incorrectly omitted 'Division of Pathology, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, Milan, 20122, Italy'. Finally, the affiliation details for Alice Turdo, Miriam Gaggianesi, Aurora Chinnici and Elisa Lipari were incorrectly given as 'Dipartimento di Biotecnologie Mediche e Medicina Legale Sezione di Biochimica Medica, Facoltà di Medicina e Chirurgia, Policlinico "P. Read More

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September 2018
2 Reads

Beeswax by-Products Efficiently Counteract the Oxidative Damage Induced by an Oxidant Agent in Human Dermal Fibroblasts.

Int J Mol Sci 2018 Sep 19;19(9). Epub 2018 Sep 19.

Dipartimento di Scienze Cliniche Specialistiche ed Odontostomatologiche (DISCO)-Sez. Biochimica, Facoltà di Medicina, Università Politecnica delle Marche, 60131 Ancona, Italy.

The antioxidant capacity and the phytochemical composition of two by-products from beeswax recycling processes were recently investigated. The aim of the present work was to evaluate the efficacy of one of these by-products, MUD1, against the oxidative stress induced by 2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH) in human dermal fibroblast (HDF) cells. After a preliminary viability assay, the protective effect of MUD1 was investigated through the measurement of apoptosis level, the reactive oxygen species (ROS) and nitrite (NO₂) production, the level of protein and lipid biomarkers (carbonyl groups, total glutathione and thiobarbituric acid-reactive substance) of oxidative damage, and the measurement of antioxidant enzymes activities (glutatione peroxidase, glutathione reductase, glutathione transferase, superoxide dismutase and catalase). Read More

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September 2018
6 Reads

Phenolic Compounds in Honey and Their Associated Health Benefits: A Review.

Molecules 2018 Sep 11;23(9). Epub 2018 Sep 11.

Dipartimento di Scienze Cliniche Specialistiche ed Odontostomatologiche (DISCO)-Sez. Biochimica, Facoltà di Medicina, Università Politecnica delle Marche, 60131 Ancona, Italy.

Honey is a natural substance appreciated for its therapeutic abilities since ancient times. Its content in flavonoids and phenolic acids plays a key role on human health, thanks to the high antioxidant and anti-inflammatory properties that they exert. Honey possesses antimicrobial capacity and anticancer activity against different types of tumors, acting on different molecular pathways that are involved on cellular proliferation. Read More

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September 2018

Protective effects of Manuka honey on LPS-treated RAW 264.7 macrophages. Part 1: Enhancement of cellular viability, regulation of cellular apoptosis and improvement of mitochondrial functionality.

Food Chem Toxicol 2018 Nov 3;121:203-213. Epub 2018 Sep 3.

Dipartimento di Scienze Cliniche Specialistiche ed Odontostomatologiche (DISCO)-Sez. Biochimica, Facoltà di Medicina, Università Politecnica delle Marche, Via Ranieri 65, 60131 Ancona, Italy. Electronic address:

Manuka honey (MH) is a monofloral honey from Australia and New Zealand, well-known for its healthy properties, such as antioxidant, antimicrobial and wound healing capacities. The aim of this work was to assess the phenolic composition and the total antioxidant capacity (TAC) of MH, as well as its effects on cellular viability, proliferation, apoptosis and metabolism in lipopolysaccharide (LPS)-treated RAW 264.7 macrophages, highlighting the molecular mechanisms involved. Read More

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November 2018
3 Reads

Corrigendum to "Sequence Analysis of the Gene in a Severe Obese Population from Southern Italy".

J Obes 2018 19;2018:3260210. Epub 2018 Jul 19.

CEINGE Biotecnologie Avanzate S.C. a R.L., Via Gaetano Salvatore 486, 80145 Naples, Italy.

[This corrects the article DOI: 10.1155/2011/269043.]. Read More

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July 2018
1 Read

Acid Derivatives of Pyrazolo[1,5-a]pyrimidine as Aldose Reductase Differential Inhibitors.

Cell Chem Biol 2018 Nov 16;25(11):1414-1418.e3. Epub 2018 Aug 16.

Dipartimento di Farmacia, Università di Pisa, Via Bonanno 6, Pisa 56126, Italy. Electronic address:

Aldose reductase (AKR1B1), the key enzyme of the polyol pathway, plays a crucial role in the development of long-term complications affecting diabetic patients. Nevertheless, the expedience of inhibiting this enzyme to treat diabetic complications has failed, due to the emergence of side effects from compounds under development. Actually AKR1B1 is a Janus-faced enzyme which, besides ruling the polyol pathway, takes part in the antioxidant defense mechanism of the body. Read More

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November 2018
8 Reads

Insights into the GTP-dependent allosteric control of c-di-GMP hydrolysis from the crystal structure of PA0575 protein from Pseudomonas aeruginosa.

FEBS J 2018 Oct 7;285(20):3815-3834. Epub 2018 Sep 7.

Department of Biochemical Sciences "A. Rossi Fanelli", Sapienza University of Rome, Italy.

Bis-(3'-5')-cyclic diguanylic acid (c-di-GMP) belongs to the class of cyclic dinucleotides, key carriers of cellular information in prokaryotic and eukaryotic signal transduction pathways. In bacteria, the intracellular levels of c-di-GMP and their complex physiological outputs are dynamically regulated by environmental and internal stimuli, which control the antagonistic activities of diguanylate cyclases (DGCs) and c-di-GMP specific phosphodiesterases (PDEs). Allostery is one of the major modulators of the c-di-GMP-dependent response. Read More

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October 2018
6 Reads

Phenolic Compounds Isolated from Olive Oil as Nutraceutical Tools for the Prevention and Management of Cancer and Cardiovascular Diseases.

Int J Mol Sci 2018 Aug 6;19(8). Epub 2018 Aug 6.

Dipartimento di Scienze Cliniche Specialistiche ed Odontostomatologiche (DISCO)-Sez. Biochimica, Facoltà di Medicina, Università Politecnica delle Marche, 60131 Ancona, Italy.

Non-communicable diseases (NCDs) have become the largest contributor to worldwide morbidity and mortality. Among them, cancer and cardiovascular diseases (CVDs) are responsible for a 47% of worldwide mortality. In general, preventive approaches modifying lifestyle are more cost-effective than treatments after disease onset. Read More

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August 2018
5 Reads

Assessment of Poly(ADP-ribose) Polymerase1 (PARP1) expression and activity in cells purified from blood and milk of dairy cattle.

Vet Immunol Immunopathol 2018 Aug 30;202:102-108. Epub 2018 Jun 30.

Consiglio per la ricerca in agricoltura e l'analisi dell'economia agraria (CREA)- Centro di ricerca Zootecnia e Acquacoltura, Via Salaria, 31- Monterotondo, Rome, Italy.

Poly(ADP-ribosyl)ation (PAR) is a post-translational protein modification catalysed by enzyme member of the poly(ADP-ribose) polymerases (PARPs) family. The activation of several PARPs is triggered by DNA strand breakage and the main PARP enzyme involved in this process is PARP1. Besides its involvement in DNA repair, PARP1 is involved in several cellular processes including transcription, epigenetics, chromatin re-modelling as well as in the maintenance of genomic stability. Read More

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August 2018
10 Reads

Protective effects of Manuka honey on LPS-treated RAW 264.7 macrophages. Part 2: Control of oxidative stress induced damage, increase of antioxidant enzyme activities and attenuation of inflammation.

Food Chem Toxicol 2018 Oct 2;120:578-587. Epub 2018 Aug 2.

Dipartimento di Scienze Cliniche Specialistiche Ed Odontostomatologiche (DISCO)-Sez. Biochimica, Facoltà di Medicina, Università Politecnica Delle Marche, 60131, Ancona, Italy. Electronic address:

The redox-system is altered by oxidative stress that is initiated by oxidative agents such as lipopolysaccharides (LPS) and reactive oxygen species (ROS), which are strongly involved in chronic inflammation. Even if Manuka honey (MH) is a good source of polyphenol rich antioxidants, its antioxidant and anti-inflammatory effects are still elusive. The aim of the present work was to explore the protective effects of MH against E. Read More

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October 2018
1 Citation
2.900 Impact Factor

Phytochemical Composition and Cytotoxic Effects on Liver Hepatocellular Carcinoma Cells of Different Berries Following a Simulated In Vitro Gastrointestinal Digestion.

Molecules 2018 Aug 1;23(8). Epub 2018 Aug 1.

Dipartimento di Scienze Cliniche Specialistiche ed Odontostomatologiche-Sez. Biochimica, Facoltà di Medicina, Università Politecnica delle Marche, 60131 Ancona, Italy.

Berry fruits are rich in nutrients and polyphenols, providing potential health benefits. Understanding the factors that affect their bioavailability is becoming of utmost importance for evaluating their biological significance and efficacy as functional food. In this study, the phytochemical composition and the total antioxidant capacity of different varieties of five berries (blackberry, blackcurrant, blueberry, raspberry, and strawberry) were evaluated after an in vitro gastrointestinal digestion process. Read More

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August 2018
5 Reads
2.420 Impact Factor

Manuka honey synergistically enhances the chemopreventive effect of 5-fluorouracil on human colon cancer cells by inducing oxidative stress and apoptosis, altering metabolic phenotypes and suppressing metastasis ability.

Free Radic Biol Med 2018 Oct 26;126:41-54. Epub 2018 Jul 26.

Dipartimento di Scienze Cliniche Specialistiche ed Odontostomatologiche (DISCO)-Sez. Biochimica, Facoltà di Medicina, Università Politecnica delle Marche, 60131 Ancona, Italy. Electronic address:

The development of chemo-sensitizers is urgently needed to overcome 5-fluorouracil (5-FU) therapeutic resistance and adverse toxicity in colorectal cancer. This work aims to evaluate the synergic effects of 5-FU and Manuka honey (MH), a rich source of bioactive compounds, in enhancing the anticancer effects of this drug on human colon cancer HCT-116 and LoVo cells. Compared to 5-FU alone, MH synergistically enhanced the chemotherapeutic effects of 5-FU, by reducing cell proliferation through the suppression of EGFR, HER2, p-Akt and p-mTOR expression, and promoting apoptosis by the modulation pro-apoptotic (p53, Bax, Cyto c, FasL caspase-3, -8, -9 and cleave-PARP) and anti-apoptotic (Bcl-2) markers. Read More

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October 2018
4 Reads
5.740 Impact Factor

Structural and conformational studies of the heparan sulfate mimetic PI-88.

Glycobiology 2018 10;28(10):731-740

Istituto Scientifico di Chimica e Biochimica "G. Ronzoni", Milan, Italy.

The heparan sulfate mimetic PI-88 is a complex mixture of sulfated oligosaccharides with anti-metastatic and anti-angiogenic activity due to its potent inhibition of heparanase and heparan sulfate-dependent angiogenic growth factors. It was recently in Phase III clinical trials for postresection hepatocellular carcinoma. The major oligosaccharide constituents of PI-88 were prepared for the first time by sulfonation of individually purified phosphorylated oligosaccharides isolated from the PI-88 precursor. Read More

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October 2018
11 Reads

Strawberry and Achenes Hydroalcoholic Extracts and Their Digested Fractions Efficiently Counteract the AAPH-Induced Oxidative Damage in HepG2 Cells.

Int J Mol Sci 2018 Jul 26;19(8). Epub 2018 Jul 26.

Dipartimento di Scienze Cliniche Specialistiche ed Odontostomatologiche (DISCO)-Sez. Biochimica, Facoltà di Medicina, Università Politecnica delle Marche, 60131 Ancona, Italy.

Strawberry fruits are highly appreciated by consumers worldwide due to their bright red color, typical aroma, and juicy texture. While the biological activity of the complete fruit has been widely studied, the potential beneficial effects of the achenes (commonly named seeds) remain unknown. In addition, when raw fruit and achenes are consumed, the digestion process could alter the release and absorption of their phytochemical compounds, compromising their bioactivity. Read More

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July 2018
1 Read
2.860 Impact Factor

Osteo-regeneration personalized for children by rapid maxillary expansion: an imaging study based on synchrotron radiation microtomography.

BMC Oral Health 2018 Jul 25;18(1):125. Epub 2018 Jul 25.

Department of Biomedical Sciences, Dentistry and Morphological and Functional Imaging, University of Messina, Messina, Italy.

Background: Personalized maxillary expansion procedure has been proposed to correct maxillary transversal deficiency; different protocols of stem cell activation have been suggested and rapid maxillary expansion (RME) is the most commonly used among clinicians. The present study aimed to quantify in three-dimensions (3D) the osteo-regeneration of the midpalatal suture in children submitted to RME.

Methods: Three patients (mean age 8. Read More

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July 2018
1 Read

Cytosolic 5'-Nucleotidase II Silencing in a Human Lung Carcinoma Cell Line Opposes Cancer Phenotype with a Concomitant Increase in p53 Phosphorylation.

Int J Mol Sci 2018 Jul 20;19(7). Epub 2018 Jul 20.

Unità di Biochimica, Dipartimento di Biologia, Università di Pisa, Via San Zeno 51, 56127 Pisa, Italy.

Purine homeostasis is maintained by a purine cycle in which the regulated member is a cytosolic 5'-nucleotidase II (cN-II) hydrolyzing IMP and GMP. Its expression is particularly high in proliferating cells, indeed high cN-II activity or expression in hematological malignancy has been associated to poor prognosis and chemoresistance. Therefore, a strong interest has grown in developing cN-II inhibitors, as potential drugs alone or in combination with other compounds. Read More

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July 2018
1 Read

Comparison of the T-cell response to human cytomegalovirus (HCMV) as detected by cytokine flow cytometry and QuantiFERON-CMV assay in HCMV-seropositive kidney transplant recipients.

New Microbiol 2018 07 20;41(3):195-202. Epub 2018 Jul 20.

Laboratorio Genetica - Trapiantologia e Malattie cardiovascolari, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy.

Human cytomegalovirus (HCMV)-specific T-cell response in kidney transplant recipients (KTR) helps to identify patients at risk for severe infection. To assess the T-cell response, this study compared our in-house developed reference test, based on T-cell (both CD4+ and CD8+) stimulation by autologous HCMV-infected dendritic cells (iDC) and subsequent detection by cytokine flow cytometry (CFC-iDC), with the Quanti-FERON-CMV (QF-CMV) assay. Fifty-three HCMV-seropositive KTR were enrolled. Read More

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July 2018
1 Read

Heterogeneity within the PF-EPN-B ependymoma subgroup.

Acta Neuropathol 2018 Aug 17;136(2):227-237. Epub 2018 Jul 17.

Programme in Developmental and Stem Cell Biology, The Hospital for Sick Children, Toronto, ON, Canada.

Posterior fossa ependymoma comprise three distinct molecular variants, termed PF-EPN-A (PFA), PF-EPN-B (PFB), and PF-EPN-SE (subependymoma). Clinically, they are very disparate and PFB tumors are currently being considered for a trial of radiation avoidance. However, to move forward, unraveling the heterogeneity within PFB would be highly desirable. Read More

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August 2018
10 Reads
10.760 Impact Factor

A multicenter study for the evaluation of the reference interval for TSH in Italy (ELAS TSH Italian Study).

Clin Chem Lab Med 2018 Jul 17. Epub 2018 Jul 17.

Università degli Studi di Genova, Genova, Italy.

Background The aims of this study were: (1) to calculate reliable thyroid stimulating hormone (TSH) reference intervals using laboratory databases; (2) to evaluate the relationship between TSH, sex and age values in different large Italian populations. Methods The TSH values stored in the laboratory information system of clinical laboratories of four Italian city hospitals, including 146,801 TSH measurements (with the respective age and sex data of individuals) were taken in consideration. Assuming a log-normal distribution, to log-transformed TSH values were applied the Dixon's iterative principle in order to exclude the outliers. Read More

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July 2018
3 Reads

Effects of Leaves Extract on High Glucose-Induced Metabolic Changes in HepG2 Cells.

Biology (Basel) 2018 Jun 26;7(3). Epub 2018 Jun 26.

Facultad de Ciencias Químicas, Universidad Juárez del Estado de Durango Campus Gómez Palacio, Avenida Artículo 123 S/N, Fracc, Filadelfia, 35010 Gómez Palacio, Mexico.

Mitochondrial dysfunction is a hallmark of diabetes, but the metabolic alterations during early stages of the disease remain unknown. The ability of liver cells to rearrange their metabolism plays an important role in compensating the energy shortage and may provide cell survival. leaves have been studied for its health properties against diabetes, insulin resistance, and non-alcoholic liver disease. Read More

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June 2018
12 Reads

Autophagy in Human Health and Disease: Novel Therapeutic Opportunities.

Antioxid Redox Signal 2018 Aug 10. Epub 2018 Aug 10.

1 Dipartimento di Scienze Cliniche Specialistiche ed Odontostomatologiche-Sez. Biochimica , Facoltà di Medicina, Università Politecnica delle Marche , Ancona, Italy .

Significance: In eukaryotes, autophagy represents a highly evolutionary conserved process, through which macromolecules and cytoplasmic material are degraded into lysosomes and recycled for biosynthetic or energetic purposes. Dysfunction of the autophagic process has been associated with the onset and development of many human chronic pathologies, such as cardiovascular, metabolic, and neurodegenerative diseases as well as cancer. Recent Advances: Currently, comprehensive research is being carried out to discover new therapeutic agents that are able to modulate the autophagic process in vivo. Read More

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August 2018
11 Reads

Corrigendum to "Interleukin-1β and tumor necrosis factor-α augment acidosis-induced rat articular chondrocyte apoptosis via nuclear factor-kappaB-dependent upregulation of ASIC1a channel" [Biochimica et Biophysica Acta 1864/1 (2018) 162-177].

Biochim Biophys Acta Mol Basis Dis 2019 Jan 11;1865(1):263-264. Epub 2018 Jun 11.

Anhui Key Laboratory of Bioactivity of Natural Products, School of Pharmacy, Anhui Medical University, Hefei 230032, China; The Key Laboratory of Anti-inflammatory and Immune Medicine, Anhui Medical University, Ministry of Education, Hefei 230032, China. Electronic address:

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January 2019
1 Read

Human iPSC-based models highlight defective glial and neuronal differentiation from neural progenitor cells in metachromatic leukodystrophy.

Cell Death Dis 2018 Jun 13;9(6):698. Epub 2018 Jun 13.

San Raffaele Telethon Institute for Gene Therapy (SR-Tiget), IRCCS San Raffaele Scientific Institute, Via Olgettina 58, 20132, Milano, Italy.

The pathological cascade leading from primary storage to neural cell dysfunction and death in metachromatic leukodystrophy (MLD) has been poorly elucidated in human-derived neural cell systems. In the present study, we have modeled the progression of pathological events during the differentiation of patient-specific iPSCs to neuroepithelial progenitor cells (iPSC-NPCs) and mature neurons, astrocytes, and oligodendrocytes at the morphological, molecular, and biochemical level. We showed significant sulfatide accumulation and altered sulfatide composition during the differentiation of MLD iPSC-NPCs into neuronal and glial cells. Read More

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June 2018
2 Reads
5.010 Impact Factor

Corrigendum to "Mitigation of NADH:ubiquinone oxidoreductase deficiency by chronic Trolox treatment" [Biochimica et Biophysica Acta 1777/7-8 (2008) 853-859].

Biochim Biophys Acta Bioenerg 2018 Dec 6;1859(12):1328. Epub 2018 Jun 6.

Department of Biochemistry, Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen Medical Centre, The Netherlands; Microscopical Imaging Centre, Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen Medical Centre, The Netherlands.

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December 2018
2 Reads

Multi-omics profiling reveals a distinctive epigenome signature for high-risk acute promyelocytic leukemia.

Oncotarget 2018 May 22;9(39):25647-25660. Epub 2018 May 22.

Department of Molecular Biology, Radboud University, Nijmegen, Netherlands.

Epigenomic alterations have been associated with both pathogenesis and progression of cancer. Here, we analyzed the epigenome of two high-risk APL (hrAPL) patients and compared it to non-high-risk APL cases. Despite the lack of common genetic signatures, we found that human hrAPL blasts from patients with extremely poor prognosis display specific patterns of histone H3 acetylation, specifically hyperacetylation at a common set of enhancer regions. Read More

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May 2018
11 Reads
6.360 Impact Factor

Corrigendum to "Tensin1 positively regulates RhoA activity through its interaction with DLC1" [Biochimica et Biophysica Acta 1853/12 (2015) 3258-3265].

Biochim Biophys Acta Mol Cell Res 2018 09 2;1865(9):1383. Epub 2018 Jun 2.

Department of Biochemistry and Molecular Medicine, University of California-Davis, Sacramento, CA 95817, USA. Electronic address:

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September 2018
1 Read

Nutraceuticals in Periodontal Health: A Systematic Review on the Role of Vitamins in Periodontal Health Maintenance.

Molecules 2018 May 20;23(5). Epub 2018 May 20.

Department of Physiology, Institute of Nutrition and Food Technology "Jose Mataix", Biomedical Research Center, University of Granada, Avda. Conocimiento s/n, 18100 Armilla, Granada, Spain.

Periodontal disease, a relevant public health problem worldwide, is generally considered a common pathology of elderly people. In this respect, there is agreement about that nutritional status may be a modifying factor in the progression and healing of the periodontal tissues. Vitamins have been recommended as nutraceuticals for prevention and treatment of some pathological conditions, such as cardiovascular diseases, obesity or cancer. Read More

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May 2018
1 Read