966 results match your criteria Biochimica Et Biophysica Acta-biomembranes[Journal]


Miltefosine inhibits the membrane remodeling caused by phospholipase action by changing membrane physical properties.

Biochim Biophys Acta Biomembr 2020 Jul 3:183407. Epub 2020 Jul 3.

Departamento de Química Biológica Ranwel Caputto, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Córdoba, Argentina; Centro de Investigaciones en Química Biológica de Córdoba (CIQUIBIC), CONICET, Haya de la Torre y Medina Allende, Ciudad Universitaria, X5000HUA Córdoba, Argentina. Electronic address:

Miltefosine (hexadecylphosphocholine or HePC) is an alkylphosphocholine approved for the treatment of visceral and cutaneous Leishmaniasis. HePC exerts its effect by interacting with lipid membranes and affecting membrane-dependent processes. The molecular geometry of HePC suggests that the pharmacological function of HePC is to alter membrane curvature. Read More

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http://dx.doi.org/10.1016/j.bbamem.2020.183407DOI Listing

Bacillus subtilis cardiolipin protects its own membrane against surfactin-induced permeabilization.

Biochim Biophys Acta Biomembr 2020 Jun 25:183405. Epub 2020 Jun 25.

Department of Genetics and Microbiology, Faculty of Science, Charles University, Viničná 5, 128 00 Prague, Czech Republic. Electronic address:

Surfactin, a cyclic lipoheptapeptide produced by Bacillus subtilis, is a surface-active antimicrobial that targets the barrier function of lipid membranes. It inserts itself into the membrane, where it forms conductive pores. Depending on its concentration, it eventually disintegrates the membrane in a detergent-like manner. Read More

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http://dx.doi.org/10.1016/j.bbamem.2020.183405DOI Listing

H nuclear magnetic resonance spectroscopy supports larger amplitude fast motion and interference with lipid chain ordering for membrane that contains β sheet human immunodeficiency virus gp41 fusion peptide or helical hairpin influenza virus hemagglutinin fusion peptide at fusogenic pH.

Biochim Biophys Acta Biomembr 2020 Jun 23;1862(10):183404. Epub 2020 Jun 23.

Department of Chemistry, Michigan State University, East Lansing, MI 48824, USA. Electronic address:

Enveloped viruses are surrounded by a membrane which is obtained from an infected host cell during budding. Infection of a new cell requires joining (fusion) of the virus and cell membranes. This process is mediated by a monotopic viral fusion protein with a large ectodomain outside the virus. Read More

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http://dx.doi.org/10.1016/j.bbamem.2020.183404DOI Listing

Enhancement of direct membrane penetration of arginine-rich peptides by polyproline II helix structure.

Biochim Biophys Acta Biomembr 2020 Jun 23;1862(10):183403. Epub 2020 Jun 23.

Department of Biophysical Chemistry, Kyoto Pharmaceutical University, 5 Misasagi-Nakauchi-cho, Yamashina-ku, Kyoto 607-8414, Japan.

The left-handed, extended polyproline II (PPII) helix is a unique secondary structure which potently modulates peptide/protein functions through its constraint conformation. To investigate the effect of PPII helix on the direct cell membrane penetration of arginine-rich peptides, we designed a polyproline-containing arginine-rich peptide P9R7W (PPPPPPPPPRRRRRRRW) by introducing nine proline residues into a linear R7W (RRRRRRRW) peptide. Circular dichroism spectroscopy showed that P9R7W has the PPII helix structure in solution whereas R7W is predominantly in random coil structure. Read More

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http://dx.doi.org/10.1016/j.bbamem.2020.183403DOI Listing

Interplay of hydrophobic and hydrophilic interactions in sequence-dependent cell penetration of spontaneous membrane-translocating peptides revealed by bias-exchange metadynamics simulations.

Biochim Biophys Acta Biomembr 2020 Jun 20;1862(10):183402. Epub 2020 Jun 20.

Shandong Provincial Key Laboratory of Biophysics, Institute of Biophysics, Dezhou University, Dezhou 253023, China; Institute for Glycomics, School of Information and Communication Technology, Griffith University, Parklands Dr, Southport, QLD 4222, Australia. Electronic address:

Spontaneous Membrane Translocating Peptides (SMTPs) can translocate silently across the bilayer and, thus, have the best potential to improve the delivery of therapeutic molecules to cells without toxicity. However, how their translocation mechanisms are affected by a specific peptide sequence remains poorly understood. Here, bias-exchange metadynamics simulations were employed to investigate the translocation mechanisms of five SMTPs with the same composition of amino acids (LLRLR, LRLLR, LLLRR, RLLLR, and LRLRL). Read More

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http://dx.doi.org/10.1016/j.bbamem.2020.183402DOI Listing

Lipid domain formation and membrane shaping by C24-ceramide.

Biochim Biophys Acta Biomembr 2020 Jun 18:183400. Epub 2020 Jun 18.

Research Institute for Medicines (iMed.ULisboa), Faculdade de Farmácia, Universidade de Lisboa, Lisboa, Portugal. Electronic address:

Ceramides are an important group of sphingolipids that modulate several cellular events. The mechanisms underlying biological actions of ceramides are not fully known, but evidence suggests that ceramides can act through regulation of the biophysical properties of the membrane. However, ceramide-induced changes on membrane properties are complex and depend on several factors. Read More

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http://dx.doi.org/10.1016/j.bbamem.2020.183400DOI Listing

The pore domain in glutamate-gated ion channels: Structure, drug binding and similarity with potassium channels.

Biochim Biophys Acta Biomembr 2020 Jun 17;1862(10):183401. Epub 2020 Jun 17.

I.M. Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, 44 Thorez Prospect, St. Petersburg 194223, Russia; Department of Biochemistry and Biomedical Sciences, McMaster University, 1280 Main Street West, Hamilton, Ontario L8N 4K1, Canada.

Ionotropic glutamate receptors in the CNS excitatory synapses of vertebrates are involved in numerous physiological and pathological processes. Decades of intensive studies greatly advanced our understanding of molecular organization of these transmembrane proteins. Here we focus on the channel pore domain, its selectivity filter and the activation gate, and the pore block by organic ligands. Read More

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http://dx.doi.org/10.1016/j.bbamem.2020.183401DOI Listing

Discovery and mechanistic characterization of a structurally-unique membrane active peptide.

Biochim Biophys Acta Biomembr 2020 Jun 18;1862(10):183394. Epub 2020 Jun 18.

Department of Biochemistry and Molecular Medicine, School of Medicine, University of California, Davis, United States of America; Department of Chemistry, University of California, Davis, United States of America. Electronic address:

Membrane active peptides (MAPs) have gained wide interest due to their far reaching applications in drug discovery and drug delivery. The search for new MAPs, however, has been largely skewed with bias selecting for physicochemical parameters believed to be important for membrane activity, such as alpha helicity, cationicity and hydrophobicity. Here we carry out a search-and-find strategy to screen a 100,000-membered one-bead-one-compound (OBOC) combinatorial peptide library for lead compounds, agnostic of those physicochemical constraints. Read More

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http://dx.doi.org/10.1016/j.bbamem.2020.183394DOI Listing
June 2020
3.836 Impact Factor

The vertebrate epithelial apical junctional complex: Dynamic interplay between Rho GTPase activity and cell polarization processes.

Biochim Biophys Acta Biomembr 2020 Jun 17;1862(10):183398. Epub 2020 Jun 17.

Program of Tissue and Organ Homeostasis, Cellular Architecture, and Organogenesis Unit, Centro de Biología Molecular Severo Ochoa, CSIC-UAM, Madrid, Spain. Electronic address:

Epithelial tissues are made of highly specialized cells present in many organs and represent the first barrier of protection from the external environment. Essential for this critical role in protection is their capacity to polarize in the apicobasal axis. The integrity of the epithelium and its properties as a protective barrier is mostly regulated by dynamic intercellular junctions composed of multiprotein complexes. Read More

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http://dx.doi.org/10.1016/j.bbamem.2020.183398DOI Listing

Scaffolding proteins of vertebrate apical junctions: structure, functions and biophysics.

Biochim Biophys Acta Biomembr 2020 Jun 15;1862(10):183399. Epub 2020 Jun 15.

Department of Cell Biology, Faculty of Sciences, University of Geneva, 4 Boulevard d'Yvoy, CH-1211 Geneva, Switzerland. Electronic address:

Tight and adherens junctions are specialized sites of cell-cell interaction in epithelia and endothelia, and are involved in barrier, adhesion, and signaling functions. These functions are orchestrated by a highly organized meshwork of macromolecules in the membrane and cytoplasmic compartments. In this review, we discuss the structural organization and functions of the major cytoplasmic scaffolding and adaptor proteins of vertebrate apical junctions (ZO proteins, afadin, PLEKHA7, cingulin, paracingulin, polarity complex proteins, and a few others), focusing on their interactions with cytoskeletal and signaling proteins. Read More

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http://dx.doi.org/10.1016/j.bbamem.2020.183399DOI Listing

The helix 0 of endophilin modifies membrane material properties and induces local curvature.

Biochim Biophys Acta Biomembr 2020 Jun 11;1862(10):183397. Epub 2020 Jun 11.

Department of Physics, University of South Florida, Tampa, FL 33620, United States of America. Electronic address:

The amphipathic helix 0 of endophilin (i.e., H0-Endo) is important to membrane binding, but its function of curvature generation remains controversial. Read More

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http://dx.doi.org/10.1016/j.bbamem.2020.183397DOI Listing

Differential adaptations in rod outer segment disc membranes in different models of congenital stationary night blindness.

Biochim Biophys Acta Biomembr 2020 Jun 11;1862(10):183396. Epub 2020 Jun 11.

Department of Ophthalmology and Visual Sciences, Case Western Reserve University, Cleveland, OH 44106, USA. Electronic address:

Rod photoreceptor cells initiate scotopic vision when the light receptor rhodopsin absorbs a photon of light to initiate phototransduction. These photoreceptor cells are exquisitely sensitive and have adaptive mechanisms in place to maintain optimal function and to overcome dysfunctional states. One adaptation rod photoreceptor cells exhibit is in the packing properties of rhodopsin within the membrane. Read More

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http://dx.doi.org/10.1016/j.bbamem.2020.183396DOI Listing

DAPTOMYCIN, its membrane-active mechanism vs. that of other antimicrobial peptides.

Authors:
Huey W Huang

Biochim Biophys Acta Biomembr 2020 Jun 9;1862(10):183395. Epub 2020 Jun 9.

Department of Physics and Astronomy, Rice University, Houston, TX, USA. Electronic address:

Over 3000 membrane-active antimicrobial peptides (AMPs) have been discovered, but only three of them have been approved by the U.S. Food and Drug Administration (FDA) for therapeutic applications, i. Read More

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http://dx.doi.org/10.1016/j.bbamem.2020.183395DOI Listing

Effect of betulin and betulonic acid on isolated rat liver mitochondria and liposomes.

Biochim Biophys Acta Biomembr 2020 Jun 6;1862(10):183383. Epub 2020 Jun 6.

Mari State University, pl. Lenina 1, Yoshkar-Ola, Mari El, 424001, Russia; Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Institutskaya 3, Pushchino, Moscow Region, 142290, Russia.

The paper considers the effects of plant triterpenoid betulin and its derivative betulonic acid on rat liver mitochondria and liposomes. It was found that betulonic acid and, to a lesser extent, betulin, activate mitochondrial respiration in states 2 and 4 and inhibit ADP- and DNP-stimulated (uncoupled) respiration. The effect of betulonic acid resulted in a significant decrease of the respiratory control and ADP/O ratios and decrease in Δψ. Read More

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http://dx.doi.org/10.1016/j.bbamem.2020.183383DOI Listing

Physiological roles of transverse lipid asymmetry of animal membranes.

Biochim Biophys Acta Biomembr 2020 Jun 6;1862(10):183382. Epub 2020 Jun 6.

School of Chemistry, University of Sydney, Sydney, NSW 2006, Australia.

The plasma membrane phospholipid distribution of animal cells is markedly asymmetric. Phosphatidylserine (PS) and phosphatidylethanolamine (PE) are concentrated in the inner leaflet, whereas phosphatidylcholine (PC) and sphingomyelin (SM) are concentrated in the outer leaflet. This non-equilibrium situation is maintained by lipid pumps (flippases or floppases), which utilize energy in the form of ATP to translocate lipids from one leaflet to the other. Read More

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http://dx.doi.org/10.1016/j.bbamem.2020.183382DOI Listing

Effect of membrane potential on pore formation by the antimicrobial peptide magainin 2 in lipid bilayers.

Biochim Biophys Acta Biomembr 2020 Jun 3;1862(10):183381. Epub 2020 Jun 3.

Integrated Bioscience Section, Graduate School of Science and Technology, Shizuoka University, Shizuoka 422-8529, Japan; Nanomaterials Research Division, Research Institute of Electronics, Shizuoka University, Shizuoka 422-8529, Japan; Department of Physics, Faculty of Science, Shizuoka University, Shizuoka 422-8529, Japan. Electronic address:

The effect of membrane potential on plasma membrane damage generated by antimicrobial peptides (AMPs) is an important, yet poorly characterized, process. Here, we studied the effect of membrane potential (φ) on pore formation by magainin 2 (Mag) in single giant unilamellar vesicles (GUVs) composed of dioleoylphosphatidylglycerol (DOPG)/dioleoylphosphatidylcholine (DOPC) membranes. Various membrane potentials in GUVs containing gramicidin A were generated as a result of K concentration gradients. Read More

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http://dx.doi.org/10.1016/j.bbamem.2020.183381DOI Listing

Oligomerization analysis as a tool to elucidate the mechanism of EBV latent membrane protein 1 inhibition by pentamidine.

Biochim Biophys Acta Biomembr 2020 Jun 1;1862(10):183380. Epub 2020 Jun 1.

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences RAS, str. Miklukho-Maklaya 16/10, Moscow 117997, Russian Federation. Electronic address:

Latent membrane protein 1 (LMP1) is a gene product of the Epstein-Barr virus (EBV), a widely spread virus present in 90-95% of the world's population. EBV can lead to several malignancies, in which LMP1 was shown to play a key role. LMP1 is active only in the oligomeric form and its fifth transmembrane domain (TMD-5) is critical for the oligomerization, with D150 identified as a key residue for LMP1 activation. Read More

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http://dx.doi.org/10.1016/j.bbamem.2020.183380DOI Listing
June 2020
3.836 Impact Factor

Spicule movement on RBCs during echinocyte formation and possible segregation in the RBC membrane.

Biochim Biophys Acta Biomembr 2020 May 30;1862(10):183338. Epub 2020 May 30.

Institute for Transfusion Medicine and Immunology, Flowcore Mannheim, Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany. Electronic address:

We use phase contrast microscopy of red blood cells to observe the transition between the initial discocyte shape and a spiculated echinocyte form. During the early stages of this change, spicules can move across the surface of the cell; individual spicules can also split apart into pairs. One possible explanation of this behaviour is that the membrane forms large scale domains in association with the spicules. Read More

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http://dx.doi.org/10.1016/j.bbamem.2020.183338DOI Listing

Magnetically-orientable Tween-based model membranes for NMR studies of proteins.

Biochim Biophys Acta Biomembr 2020 May 29;1862(10):183379. Epub 2020 May 29.

Department of Chemistry, Université du Québec à Montréal, P.O. Box 8888, Montréal, QC, H3C 3P8, Canada. Electronic address:

We present a new membrane mimetic system using a membrane softening detergent commonly known as Tween 80 (TW80), to form oriented systems for solid-state NMR applications. TW80 is a fatty acid ester (oleate) of sorbitan polyethoxylate and a mild non-ionic surfactant. Phosphatidylcholine (PC)/TW80 model membrane systems were characterized by solid-state NMR and FTIR spectroscopy. Read More

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http://dx.doi.org/10.1016/j.bbamem.2020.183379DOI Listing

The insecticide fipronil affects the physical properties of model membranes: A combined experimental and molecular dynamics simulations study in Langmuir monolayers.

Biochim Biophys Acta Biomembr 2020 May 25;1862(10):183378. Epub 2020 May 25.

Universidad Nacional de Córdoba, Facultad de Ciencias Exactas, Físicas y Naturales, Departamento de Química, Cátedra de Química Biológica, Córdoba, Argentina; Instituto de Investigaciones Biológicas y Tecnológicas, Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad Nacional de Córdoba, Córdoba, Argentina. Electronic address:

Fipronil is a widely used commercial insecticide whose action mechanism consists in blocking the influx of chloride ions through the γ-aminobutyric acid type A receptor (GABA-R), an integral membrane protein. The present study investigates the interaction of fipronil with phospholipid Langmuir monolayers, in order to characterize the effects that its partition could exert on the physical properties of these model membranes. A combined experimental and molecular dynamics (MD) simulations approach was performed. Read More

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http://dx.doi.org/10.1016/j.bbamem.2020.183378DOI Listing

Phase separation behavior of binary mixture of photopolymerizable diacetylene and unsaturated phospholipids in liposomes.

Biochim Biophys Acta Biomembr 2020 Sep 25;1862(9):183377. Epub 2020 May 25.

Center for Membrane and Film Technology, Department of Chemical Science and Engineering, Kobe University, 1-1 Rokkodai, Nada, Kobe 657-8501, Japan. Electronic address:

Photopolymerizable lipids, such as diacetylene lipids have attracted much attention for their ability to stabilize lipid bilayer structure. In this study, we investigated the phase separation behavior of a lipid bilayer containing 1,2‑bis(10,12‑tricosadiynoyl)‑sn‑glycero‑3‑phosphocholine (DiynePC). The phase separation behavior of liposomes composed of DiynePC and the non-polymerizable lipid, 1,2‑dioleoyl‑sn‑glycero‑3‑phosphocholine (DOPC) was evaluated using a fluorescence resonance energy transfer (FRET) assay during both the cooling and heating processes. Read More

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http://dx.doi.org/10.1016/j.bbamem.2020.183377DOI Listing
September 2020

The effect of hydrophobic alkyl sidechains on size and solution behaviors of nanodiscs formed by alternating styrene maleamic copolymer.

Biochim Biophys Acta Biomembr 2020 May 23;1862(10):183360. Epub 2020 May 23.

Department of Biochemistry, University of Alberta, Edmonton, AB T6G 2H7, Canada. Electronic address:

The development of amphipathic polymers, including various formulations of styrene-maleic acid (SMA) copolymers, has allowed the purification of increasing sizes and complexities of biological membrane protein assemblies in native nanodiscs. However, the factors determining the sizes and shapes of the resulting bio-nano particles remain unclear. Here, we show how grafting on short alkyl amine sidechains onto the polar residues leads to a broad set of nanoparticle sizes with improved solution behavior. Read More

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http://dx.doi.org/10.1016/j.bbamem.2020.183360DOI Listing

Molecular organization in mixed SOPC and SDPC model membranes: Water permeability studies of polyunsaturated lipid bilayers.

Biochim Biophys Acta Biomembr 2020 Sep 23;1862(9):183365. Epub 2020 May 23.

Department of Chemistry, Iona College, 715 North Avenue, New Rochelle, NY 10801, USA. Electronic address:

Polyunsaturated phospholipids in plasma membranes modulate order and dynamics of the lipid bilayer and influence integral membrane protein function. In this study, we investigate the effects of polyunsaturated fatty acid (PUFA) chains in phosphatidylcholine (PC) on osmotic water permeability and packing characteristics, using a droplet interface bilayer (DIB) as a model cell membrane. The lipid bilayer system is composed of mixtures of PC lipids with varying ratio of 1-stearoyl-2-oleoylphosphatidylcholine (SOPC, 18: 0-18:1 PC) to 1-stearoyl-2-docosahexaenoylphosphatidylcholine (SDPC, 18:0-22:6 PC). Read More

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http://dx.doi.org/10.1016/j.bbamem.2020.183365DOI Listing
September 2020

Central residues of the amphipathic β-hairpin loop control the properties of Clostridium perfringens epsilon-toxin channel.

Biochim Biophys Acta Biomembr 2020 Sep 22;1862(9):183364. Epub 2020 May 22.

Institut Pasteur, Bacterial Toxins, 28 rue du Dr Roux, 75724 Paris cedex 15, France. Electronic address:

Clostridium perfringens epsilon toxin (ETX) is a heptameric pore-forming toxin of the aerolysin toxin family. ETX is the most potent toxin of this toxin family and the third most potent bacterial toxin with high cytotoxic and lethal activities in animals. In addition, ETX shows a demyelinating activity in nervous tissue leading to devastating multifocal central nervous system white matter disease in ruminant animals. Read More

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http://dx.doi.org/10.1016/j.bbamem.2020.183364DOI Listing
September 2020

The effects of melatonin, serotonin, tryptophan and NAS on the biophysical properties of DPPC monolayers.

Biochim Biophys Acta Biomembr 2020 Sep 22;1862(9):183363. Epub 2020 May 22.

Department of Physics and Astronomy, University of Waterloo, Waterloo, Canada; Department of Biology, University of Waterloo, Waterloo, Canada. Electronic address:

Melatonin is a neurohormone that has been shown to be protective in Alzheimer's diseases against amyloid-β (Aβ) toxicity, which involves interaction of Aβ with neuronal membrane. Non-specific interactions of melatonin with cell membrane may play a physiological role in this process by preserving membrane fluidity. In the brain, melatonin is derived from the amino acid tryptophan through a pathway that includes serotonin and N-acetylserotonin (NAS). Read More

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http://dx.doi.org/10.1016/j.bbamem.2020.183363DOI Listing
September 2020

Dual benefit of combined neuroprotection: Cholesterol depletion restores membrane microviscosity but not lipid order and enhances neuroprotective action of hypothermia in rat cortex nerve terminals.

Biochim Biophys Acta Biomembr 2020 Sep 21;1862(9):183362. Epub 2020 May 21.

Dep. of Neurochemistry of the Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, 9 Leontovicha Str., Kyiv 01054, Ukraine.

Here, both neuroprotectants, i.e. cholesterol depletion of the plasma membrane of rat brain nerve terminals (synaptosomes) using methyl-β-cyclodextrin (MβCD) and deep/propound hypothermia, were analyzed during their combined administration and regarding additive neuroprotective effect. Read More

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http://dx.doi.org/10.1016/j.bbamem.2020.183362DOI Listing
September 2020

Channel functions of claudins in the organization of biological systems.

Biochim Biophys Acta Biomembr 2020 Sep 20;1862(9):183344. Epub 2020 May 20.

Institute of Clinical Physiology/Nutritional Medicine, Medical Department, Division of Gastroenterology, Infectiology, Rheumatology, Charité - Universitätsmedizin Berlin, 12203 Berlin, Germany. Electronic address:

Claudins are tight junction proteins mostly appreciated in their function of paracellular barrier-formation. Compared to a virtual absence of any tight junctions, their paracellular sealing role certainly stands out. Yet, it was recognized immediately after the discovery of the first claudins, that some members of the claudin protein family were able to convey size and charge selectivity to the paracellular pathway. Read More

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http://dx.doi.org/10.1016/j.bbamem.2020.183344DOI Listing
September 2020

Hyaluronic acid binding to CD44S is indiscriminate of molecular weight.

Biochim Biophys Acta Biomembr 2020 Sep 16;1862(9):183348. Epub 2020 May 16.

Department of Pharmaceutics and Pharmaceutical Chemistry, University of Utah, Salt Lake City, UT 84112, United States of America; Department of Biomedical Engineering, Department of Medicinal Chemistry, University of Utah, Salt Lake City, UT 84112, United States of America. Electronic address:

The ubiquitous presence of hyaluronic acid (HA) in the extracellular matrix (ECM) of both healthy and diseased tissues underscores its importance in human physiology. Previous studies suggest that HA can be used as a probe to qualitatively monitor cell surface levels of CD44 and other important HA receptors; however, these studies use mixtures of HA at various molecular weights. Using fluorescently labeled HA, we evaluated the apparent differences of low (25 kilodalton) and high (700 kilodalton) molecular weight HA interacting with breast cancer cell lines of varying levels of CD44. Read More

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http://dx.doi.org/10.1016/j.bbamem.2020.183348DOI Listing
September 2020

Synergistic inhibition mechanism of pediocin PA-1 and L-lactic acid against Aeromonas hydrophila.

Biochim Biophys Acta Biomembr 2020 May 16;1862(10):183346. Epub 2020 May 16.

Tianjin Key Laboratory of Aqua-ecology and Aquaculture, College of Fisheries, Tianjin Agricultural University, 22 Jinjing Road, 300384 Tianjin, China.

Pediocin PA-1 (PA-1) is a membrane-targeting bacteriocin from lactic acid bacteria, which shows antimicrobial activity against a wide range of Gram-positive pathogens. However, the outer membrane of Gram-negative bacteria does not allow pediocin access to its target. In this work, the synergistic inhibitory mechanism of PA-1 with L-lactic acid against Gram-negative aquaculture and food pathogen Aeromonas hydrophila (A. Read More

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http://dx.doi.org/10.1016/j.bbamem.2020.183346DOI Listing

The effect of lipid phase on liposome stability upon exposure to the mechanical stress.

Biochim Biophys Acta Biomembr 2020 Sep 15;1862(9):183361. Epub 2020 May 15.

Lipid Systems sp. z o.o., 54-613 Wrocław, ul. Krzemieniecka 48C, Poland.

Mechanical properties of a lipid bilayer are parameters determined mainly for giant unilamellar vesicles (GUVs). It is not clear if values obtained on the GUV model can be directly translated to submicron large unilamellar vesicles (LUVs). This ambiguity is a major obstacle in exploring the effect of lipid bilayer mechanics on membrane associated processes and effectiveness of liposome-based targeted drug delivery systems. Read More

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http://dx.doi.org/10.1016/j.bbamem.2020.183361DOI Listing
September 2020

Regulation and function of V-ATPases in physiology and disease.

Biochim Biophys Acta Biomembr 2020 May 16:183341. Epub 2020 May 16.

Cell, Molecular and Developmental Biology, Tufts University Graduate School of Biomedical Sciences, United States of America; Dept. of Developmental, Molecular and Chemical Biology, Tufts University School of Medicine, Boston, MA 02111, United States of America. Electronic address:

The vacuolar H-ATPases (V-ATPases) are essential, ATP-dependent proton pumps present in a variety of eukaryotic cellular membranes. Intracellularly, V-ATPase-dependent acidification functions in such processes as membrane traffic, protein degradation, autophagy and the coupled transport of small molecules. V-ATPases at the plasma membrane of certain specialized cells function in such processes as bone resorption, sperm maturation and urinary acidification. Read More

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http://dx.doi.org/10.1016/j.bbamem.2020.183341DOI Listing

Magnetically aligned membrane mimetics enabling comparable chiroptical and magnetic resonance spectroscopy studies.

Biochim Biophys Acta Biomembr 2020 Sep 14;1862(9):183343. Epub 2020 May 14.

Centre for Biological Sciences, University of Southampton, Southampton SO17 1BJ, United Kingdom. Electronic address:

Lipidic bicelles have been widely used for the analysis of integral membrane proteins where their spontaneous alignment in an magnetic field has been exploited for oriented sample solid-state NMR studies. Many of their physical properties however make them well suited to the analysis of membrane proteins by circular dichroism (CD) and synchrotron radiation circular dichroism (SRCD). In this paper we have identified bicelle compositions that permit comparable studies of integral membrane proteins by solid-state NMR, CD, and SRCD; complementary methods that can provide insights into protein structure and the interactions with drugs and other small molecules. Read More

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http://dx.doi.org/10.1016/j.bbamem.2020.183343DOI Listing
September 2020

Cholesterol and desmosterol incorporation into ram sperm membrane before cryopreservation: Effects on membrane biophysical properties and sperm quality.

Biochim Biophys Acta Biomembr 2020 Sep 13;1862(9):183357. Epub 2020 May 13.

Laboratorio Biotecnología de la Reproducción, Departamento de Producción Animal, Instituto Nacional de Tecnología Agropecuaria (INTA), EEA Balcarce, Ruta 226 km 73.5, 7620 Balcarce, Buenos Aires, Argentina. Electronic address:

Ram sperm are particularly sensitive to freeze-thawing mainly due to their lipid composition, limiting their use in artificial insemination programs. We evaluated the extent of cholesterol and desmosterol incorporation into ram sperm through incubation with increasing concentrations of methyl-β-cyclodextrin (MβCD)-sterol complexes, and its effect on membrane biophysical properties, membrane lateral organization and cryopreservation outcome. Sterols were effectively incorporated into the sperm membrane at 10 and 25 mM MβCD-sterols, similarly increasing membrane lipid order at physiological temperature and during temperature decrease. Read More

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http://dx.doi.org/10.1016/j.bbamem.2020.183357DOI Listing
September 2020

Characterization of lipid composition and diffusivity in OLA generated vesicles.

Biochim Biophys Acta Biomembr 2020 Sep 13;1862(9):183359. Epub 2020 May 13.

Cavendish Laboratory, University of Cambridge, JJ Thomson Avenue, Cambridge CB3 0HE, United Kingdom. Electronic address:

Giant Unilamellar Vesicles (GUVs) are a versatile tool in many branches of science, including biophysics and synthetic biology. Octanol-Assisted Liposome Assembly (OLA), a recently developed microfluidic technique enables the production and testing of GUVs within a single device under highly controlled experimental conditions. It is therefore gaining significant interest as a platform for use in drug discovery, the production of artificial cells and more generally for controlled studies of the properties of lipid membranes. Read More

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http://dx.doi.org/10.1016/j.bbamem.2020.183359DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7322398PMC
September 2020

Solution NMR spectroscopy of membrane proteins.

Biochim Biophys Acta Biomembr 2020 Sep 13;1862(9):183356. Epub 2020 May 13.

Department of Biochemistry, University of Alberta, Edmonton, Alberta T6G 2H7, Canada; Department of Medicine, University of Alberta, Edmonton, Alberta T6G 2H7, Canada. Electronic address:

Integral membrane proteins (IMPs) perform unique and indispensable functions in the cell, making them attractive targets for fundamental research and drug discovery. Developments in protein production, isotope labeling, sample preparation, and pulse sequences have extended the utility of solution NMR spectroscopy for studying IMPs with multiple transmembrane segments. Here we review some recent applications of solution NMR for studying structure, dynamics, and interactions of polytopic IMPs, emphasizing strategies used to overcome common technical challenges. Read More

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http://dx.doi.org/10.1016/j.bbamem.2020.183356DOI Listing
September 2020

Effect of tetracaine on dynamic reorganization of lipid membranes.

Biochim Biophys Acta Biomembr 2020 Sep 13;1862(9):183351. Epub 2020 May 13.

College of Chemistry and Chemical Engineering, Shanghai University of Engineering Science, Shanghai 201620, China.

To understand the intrinsic influence of a drug on lipid membranes is of critical importance in pharmacological science. Herein, we report fluorescence microscopy analysis of the interaction between the local anesthetic tetracaine (TTC) and planar supported lipid bilayers (SLBs), as model membranes. Our results show that TTC increases lipid chain mobility, destabilizes the SLBs and remarkably induces membrane disruption and solubilization. Read More

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http://dx.doi.org/10.1016/j.bbamem.2020.183351DOI Listing
September 2020

The SecA ATPase motor protein binds to Escherichia coli liposomes only as monomers.

Biochim Biophys Acta Biomembr 2020 Sep 19;1862(9):183358. Epub 2020 May 19.

Department of Physiology and Biophysics, School of Medicine, University of California, Irvine, Irvine, CA 92697, United States of America. Electronic address:

The essential SecA motor ATPase acts in concert with the SecYEG translocon to secrete proteins into the periplasmic space of Escherichia coli. In aqueous solutions, SecA exists largely as dimers, but the oligomeric state on membranes is less certain. Crystallographic studies have suggested several possible solution dimeric states, but its oligomeric state when bound to membranes directly or indirectly via the translocon is controversial. Read More

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http://dx.doi.org/10.1016/j.bbamem.2020.183358DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7316483PMC
September 2020

Proteasome-dependent protein quality control of the peroxisomal membrane protein Pxa1p.

Biochim Biophys Acta Biomembr 2020 Sep 13;1862(9):183342. Epub 2020 May 13.

Department of Cell Biochemistry, University of Groningen, the Netherlands. Electronic address:

Peroxisomes are eukaryotic organelles that function in numerous metabolic pathways and defects in peroxisome function can cause serious developmental brain disorders such as adrenoleukodystrophy (ALD). Peroxisomal membrane proteins (PMPs) play a crucial role in regulating peroxisome function. Therefore, PMP homeostasis is vital for peroxisome function. Read More

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http://dx.doi.org/10.1016/j.bbamem.2020.183342DOI Listing
September 2020

Optimizing the α-adrenergic receptor for solution NMR studies.

Biochim Biophys Acta Biomembr 2020 May 13;1862(10):183354. Epub 2020 May 13.

Department of Chemistry, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland. Electronic address:

Sample preparation for NMR studies of G protein-coupled receptors faces special requirements: Proteins need to be stable for prolonged measurements at elevated temperatures, they should ideally be uniformly labeled with the stable isotopes C, N, and all carbon-bound protons should be replaced by deuterons. In addition, certain NMR experiments require protonated methyl groups in the presence of a perdeuterated background. All these requirements are most easily satisfied when using Escherichia coli as the expression host. Read More

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http://dx.doi.org/10.1016/j.bbamem.2020.183354DOI Listing

Dynamics and structural communication in the ternary complex of fully phosphorylated V2 vasopressin receptor, vasopressin, and β-arrestin 1.

Biochim Biophys Acta Biomembr 2020 Sep 12;1862(9):183355. Epub 2020 May 12.

Department of Life Sciences, University of Modena and Reggio Emilia, via Campi 103, 41125 Modena, Italy; Center for Neuroscience and Neurotechnology, University of Modena and Reggio Emilia, via Campi 287, 41125 Modena, Italy. Electronic address:

G protein-coupled receptors (GPCRs) are critically regulated by arrestins, which not only desensitize G-protein signaling but also initiate a G protein-independent wave of signaling. The information from structure determination was herein exploited to build a structural model of the ternary complex, comprising fully phosphorylated V2 vasopressin receptor (V2R), the agonist arginine vasopressin (AVP), and β-arrestin 1 (β-arr1). Molecular simulations served to explore dynamics and structural communication in the ternary complex. Read More

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http://dx.doi.org/10.1016/j.bbamem.2020.183355DOI Listing
September 2020

The binding of the APT1 domains to phosphoinositides is regulated by metal ions in vitro.

Biochim Biophys Acta Biomembr 2020 Sep 11;1862(9):183349. Epub 2020 May 11.

Institute of Biochemistry and Biophysics Polish Academy of Sciences, Pawinskiego 5A, 02-106 Warsaw, Poland.

Chorein is a protein of the Vps13 family, and defects in this protein cause the rare neurodegenerative disorder chorea-acanthocytosis (ChAc). Chorein is involved in the actin cytoskeleton organization, calcium ion flux, neuronal cell excitability, exocytosis and autophagy. The function of this protein is poorly understood, and obtaining this knowledge is a key to finding a cure for ChAc. Read More

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http://dx.doi.org/10.1016/j.bbamem.2020.183349DOI Listing
September 2020

Potent in vitro and in vivo antimicrobial activity of semisynthetic amphiphilic γ-mangostin derivative LS02 against Gram-positive bacteria with destructive effect on bacterial membrane.

Biochim Biophys Acta Biomembr 2020 Sep 11;1862(9):183353. Epub 2020 May 11.

Key Laboratory of Molecular Target & Clinical Pharmacology and the State Key Laboratory of Respiratory Disease, School of Pharmaceutical Sciences & the Fifth Affiliated Hospital, Guangzhou Medical University, Guangzhou 511436, PR China. Electronic address:

Semisynthetic γ-mangostin derivative LS02 is a novel cationic amphiphilic peptidomimetic antimicrobial agent containing a hydrophobic scaffold and three hydrophilic and positively charged residues of arginine. LS02 showed low in vitro toxicity, potent activities against Gram-positive bacteria including MRSA (MIC = 1.56-6. Read More

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http://dx.doi.org/10.1016/j.bbamem.2020.183353DOI Listing
September 2020

Identifying lipids tightly bound to an integral membrane protein.

Biochim Biophys Acta Biomembr 2020 May 11;1862(10):183345. Epub 2020 May 11.

Department of Physics, University of Guelph, Guelph, ON N1G 2W1, Canada; Biophysics Interdepartmental Group, University of Guelph, Guelph, ON N1G 2W1, Canada. Electronic address:

Anabaena Sensory Rhodopsin (ASR) is a microbial photosensor from the cyanobacterium Anabaena sp. PCC 7120. It was found in previous studies that ASR co-purifies with several small molecules, although their identities and structural or functional roles remained unclear. Read More

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http://dx.doi.org/10.1016/j.bbamem.2020.183345DOI Listing

Nanomechanical characterization of single phospholipid bilayer in ripple phase with PF-QNM AFM.

Biochim Biophys Acta Biomembr 2020 Sep 11;1862(9):183347. Epub 2020 May 11.

Institute of Physical Chemistry, Polish Academy of Sciences, Kasprzaka 44/52, 01-224 Warsaw, Poland. Electronic address:

Topography and nanomechanical properties of the supported 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) single bilayer in the ripple phase were investigated by atomic force microscopy with the use of PeakForce Quantitative Nanomechanical Mapping (PF-QNM) mode under liquid conditions. The DMPC single bilayer was deposited on the mica surface by Langmuir-Blodgett and Langmuir-Schaefer techniques combined. Next, the sample was stored overnight at 4 °C under buffer solution (pH = 7. Read More

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http://dx.doi.org/10.1016/j.bbamem.2020.183347DOI Listing
September 2020

Structure and dynamics of phospholipids in membranes elucidated by combined use of NMR and vibrational spectroscopies.

Authors:
Hideo Akutsu

Biochim Biophys Acta Biomembr 2020 Sep 11;1862(9):183352. Epub 2020 May 11.

Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Suita 565-0871, Japan; Graduate School of Medical Life Science, Yokohama City University, 1-7-29 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045, Japan. Electronic address:

NMR is a sophisticated method for investigation of structure and dynamics of lipid and protein molecules in membranes. Vibrational spectroscopy is also powerful because of relatively high resolution and sensitivity, and easier access than NMR. A combined use of these spectroscopies could provide important insights into the membrane biophysics. Read More

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http://dx.doi.org/10.1016/j.bbamem.2020.183352DOI Listing
September 2020

A machine learning approach to estimation of phase diagrams for three-component lipid mixtures.

Biochim Biophys Acta Biomembr 2020 Sep 11;1862(9):183350. Epub 2020 May 11.

Department of Chemical and Biomolecular Engineering, Russ College of Engineering and Technology, Ohio University, Athens, OH 45701, USA. Electronic address:

The plasma membrane of eukaryotic cells is commonly believed to contain ordered lipid domains. The interest in understanding the origin of such domains has led to extensive studies on the phase behavior of mixed lipid systems. Three-component phase diagrams, composed of a high melting temperature (Tm) lipid, cholesterol, and a low Tm lipid have been valuable in studying lipid phase behavior. Read More

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http://dx.doi.org/10.1016/j.bbamem.2020.183350DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7301216PMC
September 2020

Ruffles and spikes: Control of tight junction morphology and permeability by claudins.

Biochim Biophys Acta Biomembr 2020 Sep 7;1862(9):183339. Epub 2020 May 7.

Division of Pulmonary, Allergy Critical Care and Sleep Medicine, Department of Medicine, Emory University School of Medicine, Atlanta, GA 30322, USA; Department of Cell Biology, Emory University School of Medicine, Atlanta, GA 30322, USA. Electronic address:

Epithelial barrier function is regulated by a family of transmembrane proteins known as claudins. Functional tight junctions are formed when claudins interact with other transmembrane proteins, cytosolic scaffold proteins and the actin cytoskeleton. The predominant scaffold protein, zonula occludens-1 (ZO-1), directly binds to most claudin C-terminal domains, crosslinking them to the actin cytoskeleton. Read More

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http://dx.doi.org/10.1016/j.bbamem.2020.183339DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7299829PMC
September 2020

Inherited disorders of lysosomal membrane transporters.

Biochim Biophys Acta Biomembr 2020 May 8:183336. Epub 2020 May 8.

Human Biochemical Genetics Section, Medical Genetics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892, USA.

Disorders caused by defects in lysosomal membrane transporters form a distinct subgroup of lysosomal storage disorders (LSDs). To date, defects in only 10 lysosomal membrane transporters have been associated with inherited disorders. The clinical presentations of these diseases resemble the phenotypes of other LSDs; they are heterogeneous and often present in children with neurodegenerative manifestations. Read More

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http://dx.doi.org/10.1016/j.bbamem.2020.183336DOI Listing

Pleiotropic effects of statins on brain cells.

Biochim Biophys Acta Biomembr 2020 Sep 6;1862(9):183340. Epub 2020 May 6.

Institute of Biomedical Research (BIOMED), Pontifical Catholic University of Argentina (UCA) and National Scientific and Technical Research Council (CONICET), Av. Alicia Moreau de Justo 1600, C1107AFF Buenos Aires, Argentina. Electronic address:

Starting with cholesterol homeostasis, the first part of the review addresses various aspects of cholesterol metabolism in neuronal and glial cells and the mutual crosstalk between the two cell types, particularly the transport of cholesterol from its site of synthesis to its target loci in neuronal cells, discussing the multiple mechanistic aspects and transporter systems involved. Statins are next analyzed from the point of view of their chemical structure and its impingement on their pharmacological properties and permeability through cell membranes and the blood-brain barrier in particular. The following section then discusses the transcriptional effects of statins and the changes they induce in brain cell genes associated with a variety of processes, including cell growth, signaling and trafficking, uptake and synthesis of cholesterol. Read More

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http://dx.doi.org/10.1016/j.bbamem.2020.183340DOI Listing
September 2020

Real-time monitoring of heat transfer between gold nanoparticles and tethered bilayer lipid membranes.

Biochim Biophys Acta Biomembr 2020 Sep 4;1862(9):183334. Epub 2020 May 4.

School of Life Sciences, University of Technology Sydney, Sydney, New South Wales 2007, Australia; Institute for Biomedical Materials and Devices, University of Technology Sydney, Sydney, New South Wales 2007, Australia; ARC Research Hub for Integrated Devices for End-user Analysis at Low-levels (IDEAL), Faculty of Science, University of Technology Sydney, NSW 2007, Australia. Electronic address:

Plasmon resonance frequency irradiated gold nanoparticles (GNPs) have gained interest as a laser-targeted treatment for infections, tumors and for the controlled release of drugs in situ. Questions still remain, however, as to the efficiency of heat delivery within biological tissues and how this can be reliably determined. Here, we demonstrate how a nanomaterial-electrode interface that mimics cell membranes can detect the localized heat transfer characteristics arising from plasmon resonance frequency-matched laser excitation of GNPs. Read More

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http://dx.doi.org/10.1016/j.bbamem.2020.183334DOI Listing
September 2020