646 results match your criteria Biochimica Et Biophysica Acta-biomembranes[Journal]


New insight into phase behavior and permeability of skin lipid models based on sphingosine and phytosphingosine ceramides.

Biochim Biophys Acta Biomembr 2019 Apr 13. Epub 2019 Apr 13.

Division BioTherapeutics, Leiden Academic Centre for Drug Research, Leiden University, Netherlands. Electronic address:

The intercellular lipid matrix of the stratum corneum (SC), which consist mainly of ceramides (CERs), free fatty acids and cholesterol, is fundamental to the skin barrier function. These lipids assemble into two lamellar phases, known as the long and short periodicity phases (LPP and SPP respectively). The LPP is unique in the SC and is considered important for the skin barrier function. Read More

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http://dx.doi.org/10.1016/j.bbamem.2019.04.005DOI Listing

Ultrashort nanosecond electric pulses evoke heterogeneous patterns of Ca release from the endoplasmic reticulum of adrenal chromaffin cells.

Biochim Biophys Acta Biomembr 2019 Apr 12. Epub 2019 Apr 12.

Department of Pharmacology, University of Nevada, Reno School of Medicine, Reno, NV 89557, USA.

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http://dx.doi.org/10.1016/j.bbamem.2019.04.006DOI Listing

Modulation of biological responses to 2 ns electrical stimuli by field reversal.

Biochim Biophys Acta Biomembr 2019 Apr 11. Epub 2019 Apr 11.

Frank Reidy Research Center for Bioelectrics, Old Dominion University, 4211 Monarch Way Suite 300, Norfolk, VA 23508, USA.

Nanosecond bipolar pulse cancellation, a recently discovered phenomenon, is modulation of the effects of a unipolar electric pulse exposure by a second pulse of opposite polarity. This attenuation of biological response by reversal of the electric field direction has been reported with pulse durations from 60 ns to 900 ns for a wide range of endpoints, and it is not observed with conventional electroporation pulses of much longer duration (>100 μs) where pulses are additive regardless of polarity. The most plausible proposed mechanisms involve the field-driven migration of ions to and from the membrane interface (accelerated membrane discharge). Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00052736193007
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http://dx.doi.org/10.1016/j.bbamem.2019.03.019DOI Listing
April 2019
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A biophysical study of the interactions between the antimicrobial peptide indolicidin and lipid model systems.

Biochim Biophys Acta Biomembr 2019 Apr 9. Epub 2019 Apr 9.

Department of Chemistry, University of Oslo, 0315 Oslo, Norway. Electronic address:

The naturally occurring peptide indolicidin from bovine neutrophils exhibits strong biological activity against a broad spectrum of microorganisms. This is believed to arise from selective interactions with the negatively charged cytoplasmic lipid membrane found in bacteria. We have investigated the peptide interaction with supported lipid model membranes using a combination of complementary surface sensitive techniques: neutron reflectometry (NR), atomic force microscopy (AFM), and quartz crystal microbalance with dissipation monitoring (QCM-D). Read More

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http://dx.doi.org/10.1016/j.bbamem.2019.04.003DOI Listing
April 2019
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Sub-inhibitory membrane damage undermines Staphylococcus aureus virulence.

Biochim Biophys Acta Biomembr 2019 Apr 8;1861(6):1172-1179. Epub 2019 Apr 8.

Department of Biotechnology and Food Engineering, Technion-Israel Institute of Technology, Haifa, Israel. Electronic address:

We investigated antibacterial properties of a recently described membrane-active lipopeptide, COOcO (decanoyl-ornithyl-ornithyl-dodecanoyl-ornithyl-amide) against Gram-positive bacteria (GPB). Minimal inhibitory concentrations (MICs) and kinetics were compared in culture media and plasma. Chemo-sensitization to antibiotics was determined using the checkerboard assay. Read More

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http://dx.doi.org/10.1016/j.bbamem.2019.04.002DOI Listing
April 2019
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The intracellular NPxY motif is critical in maintaining the function and expression of human organic anion transporting polypeptide 1B1.

Biochim Biophys Acta Biomembr 2019 Apr 7. Epub 2019 Apr 7.

College of Life Sciences, South China Agricultural University, Guangzhou, China; Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, South China Agricultural University, Guangzhou, China. Electronic address:

Organic anion transporting polypeptides (OATPs, gene symbol SLCO) mediate sodium-independent transport of endogenous compounds such as bile salts, hormones and their conjugates as well as toxins and drugs. OATP1B1 is the major OATP specifically expressed at the basolateral membrane of human hepatocytes and many clinically important drugs have been shown to be substrates of the transporter. According to the computer-based hydropathy analysis, a large intracellular loop 3 (IL3) is situated between transmembrane domain 6 and 7 of OATPs, in which a conserved NPxY motif is found. Read More

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http://dx.doi.org/10.1016/j.bbamem.2019.04.001DOI Listing

Structural and mechanistic insights into EchAMP: A antimicrobial protein from the Echidna milk.

Biochim Biophys Acta Biomembr 2019 Apr 2. Epub 2019 Apr 2.

CSIR-Centre for Cellular and Molecular Biology, Hyderabad 500007, Telangana, India. Electronic address:

Background: Antibiotic resistance is a problem that necessitates the identification of new antimicrobial molecules. Milk is known to have molecules with antimicrobial properties (AMPs). Echidna Antimicrobial Protein (EchAMP) is one such lactation specific AMP exclusively found in the milk of Echidna, an egg-laying mammal geographically restricted to Australia and New Guinea. Read More

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http://dx.doi.org/10.1016/j.bbamem.2019.03.020DOI Listing

Use of virus-like particles as a native membrane model to study the interaction of insulin with the insulin receptor.

Biochim Biophys Acta Biomembr 2019 Apr 2. Epub 2019 Apr 2.

School of Pharmacy and Biomedical Sciences, Curtin University, Bentley campus, Perth, Western Australia 6102, Australia; Curtin University, Bentley campus, Perth, Western Australia 6102, Australia. Electronic address:

There is emerging evidence of the utility of virus-like particles (VLPs) as a novel model for the study of receptor-ligand interactions in a native plasma membrane environment. VLPs consist of a viral core protein encapsulated by portions of the cell membrane with membrane proteins and receptors expressed in their native conformation. VLPs can be generated in mammalian cells by transfection with the retroviral core protein (gag). Read More

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http://dx.doi.org/10.1016/j.bbamem.2019.03.021DOI Listing

Selective antibacterial activity of the cationic peptide PaDBS1R6 against Gram-negative bacteria.

Biochim Biophys Acta Biomembr 2019 Mar 26. Epub 2019 Mar 26.

Centro de Análises Proteômicas e Bioquímicas, Pós-Graduação em Ciências Genômicas e Biotecnologia Universidade Católica de Brasília, Brasília, DF, Brazil; Programa de pós-graduação em patologia Molecular, Universidade de Brasília, Brasília, DF, Brazil; S-Inova Biotech, Pós-graduação em Biotecnologia, Universidade Católica Dom Bosco, Campo Grande, MS, Brazil. Electronic address:

Infections caused by Gram-negative bacteria, Escherichia coli and Pseudomonas aeruginosa foremost among them, constitute a major worldwide health problem. Bioinformatics methodologies are being used to rationally design new antimicrobial peptides, a potential alternative for treating these infections. One of the algorithms used to develop antimicrobial peptides is the Joker, which was used to design the peptide PaDBS1R6. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00052736193007
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http://dx.doi.org/10.1016/j.bbamem.2019.03.016DOI Listing
March 2019
6 Reads
3.836 Impact Factor

Correlation between the hydration of acyl chains and phosphate groups in lipid bilayers: Effect of phase state, head group, chain length, double bonds and carbonyl groups.

Biochim Biophys Acta Biomembr 2019 Mar 26. Epub 2019 Mar 26.

Applied Biophysics and Food Research Center (Centro de Investigaciones en Biofísica Aplicada y Alimentos, CIBAAL), National University of Santiago del Estero, RN 9 - Km 1125, 4206 Santiago del Estero, Argentina; CONICET, RN 9 - Km 1125, 4206 Santiago del Estero, Argentina. Electronic address:

This paper demonstrates by means of FTIR/ATR analysis that water molecules intercalate at different extents in the acyl chain region of lipid membranes in correlation with the hydration of the phosphate groups. This correlation is sensible to the chain length, the presence of double bonds and the phase state of the lipid membrane. The presence of carbonyl groups CO modifies the profile of hydration of the two regions as observed from the comparison of DMPC and 14:0 Diether PC. Read More

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http://dx.doi.org/10.1016/j.bbamem.2019.03.018DOI Listing
March 2019
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Action of tannin on cellular membranes: Novel insights from concerted studies on lipid bilayers and native cells.

Biochim Biophys Acta Biomembr 2019 Mar 26;1861(6):1103-1111. Epub 2019 Mar 26.

Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Moscow region 142290, Russia.

Hydrolyzable tannin (3,6-bis-O-digalloyl-1,2,4-tri-O-galloyl-β-d-glucose) has a dual effect on the cell membrane: (1) it binds to a plasmalemmal protein of the Chara corallina cell (C = 2.7 ± 0.3 μM) and (2) it forms ionic channels in the lipid membrane. Read More

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http://dx.doi.org/10.1016/j.bbamem.2019.03.017DOI Listing
March 2019
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The influence of terpinen-4-ol and eucalyptol - The essential oil components - on fungi and plant sterol monolayers.

Biochim Biophys Acta Biomembr 2019 Mar 26;1861(6):1093-1102. Epub 2019 Mar 26.

Faculty of Chemistry, Jagiellonian University, Gronostajowa 2, 30-387 Kraków, Poland. Electronic address:

Antifungal and herbicidal activity of terpenes, being the components of the essential oils, is directly related to the incorporation of these compounds into cellular membranes. Thus, the differences in the lipid composition of various pathogenic membranes may be the factor determining the activity of these molecules. One of the class of lipids, which form the membrane environment are sterols. Read More

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http://dx.doi.org/10.1016/j.bbamem.2019.03.015DOI Listing
March 2019
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The acetylcholinesterase (AChE) inhibitor and anti-Alzheimer drug donepezil interacts with human erythrocytes.

Biochim Biophys Acta Biomembr 2019 Mar 20;1861(6):1078-1085. Epub 2019 Mar 20.

Instituto de Química, Pontificia Universidad Católica de Valparaíso, Valparaíso, Chile.

Donepezil is used to treat symptomatically the Alzheimer's disease (AD). This drug is a specific inhibitor of the enzyme acetylcholinesterase (AChE), whose main physiological function is to hydrolyze the neurotransmitter acetylcholine. The main objective of this work was to study the effect of donepezil on human erythrocytes as AChE is present in its membrane. Read More

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http://dx.doi.org/10.1016/j.bbamem.2019.03.014DOI Listing

Effect of sterol structure on ordered membrane domain (raft) stability in symmetric and asymmetric vesicles.

Biochim Biophys Acta Biomembr 2019 Mar 20;1861(6):1112-1122. Epub 2019 Mar 20.

Dept. of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, NY 11794-5215, United States of America. Electronic address:

Sterol structure influences liquid ordered domains in membranes, and the dependence of biological functions on sterol structure can help identify processes dependent on ordered domains. In this study we compared the effect of sterol structure on ordered domain formation in symmetric vesicles composed of mixtures of sphingomyelin, 1, 2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and cholesterol, and in asymmetric vesicles in which sphingomyelin was introduced into the outer leaflet of vesicles composed of DOPC and cholesterol. In most cases, sterol behavior was similar in symmetric and asymmetric vesicles, with ordered domains most strongly stabilized by 7-dehydrocholesterol (7DHC) and cholesterol, stabilized to a moderate degree by lanosterol, epicholesterol and desmosterol, and very little if at all by 4-cholesten-3-one. Read More

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http://dx.doi.org/10.1016/j.bbamem.2019.03.012DOI Listing

Structural determinants of the interaction between influenza A virus matrix protein M1 and lipid membranes.

Biochim Biophys Acta Biomembr 2019 Mar 20;1861(6):1123-1134. Epub 2019 Mar 20.

University of Potsdam, Institute of Biochemistry and Biology, Karl-Liebknecht-Str. 24-25, 14476 Potsdam, Germany. Electronic address:

Influenza A virus is a pathogen responsible for severe seasonal epidemics threatening human and animal populations every year. One of the ten major proteins encoded by the viral genome, the matrix protein M1, is abundantly produced in infected cells and plays a structural role in determining the morphology of the virus. During assembly of new viral particles, M1 is recruited to the host cell membrane where it associates with lipids and other viral proteins. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00052736193006
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http://dx.doi.org/10.1016/j.bbamem.2019.03.013DOI Listing
March 2019
5 Reads

Studies on interaction of green silver nanoparticles with whole bacteria by surface characterization techniques.

Biochim Biophys Acta Biomembr 2019 Mar 19;1861(6):1086-1092. Epub 2019 Mar 19.

Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Lisbon, Portugal; Laboratorio de Compuestos Bioactivos, Centro de Investigaciones en Biofísica Aplicada y Alimentos (CIBAAL), CONICET, Universidad Nacional de Santiago del Estero, RN 9, Km 1125, 4206 Santiago del Estero, Argentina; Laboratorio de Microbiología Molecular, Instituto de Microbiología Básica y Aplicada, Universidad Nacional de Quilmes, Roque Saenz Peña 352, B1876BXD Bernal, Argentina. Electronic address:

The use of silver nanoparticles (AgNPs) with their novel and distinct physical, chemical, and biological properties, has proven to be an alternative for the development of new antibacterial agents. In particular, the possibility to generate AgNPs coated with novel capping agents, such as phytomolecules obtained via a green synthesis (G-AgNPs), is attracting great attention in scientific research. Recently, we showed that membrane interactions seem to be involved in the antibacterial activity of AgNPs obtained via a green chemical synthesis using the aqueous leaf extract of chicory (Cichorium intybus L. Read More

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http://dx.doi.org/10.1016/j.bbamem.2019.03.011DOI Listing
March 2019
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The effect of chlorination degree and substitution pattern on the interactions of polychlorinated biphenyls with model bacterial membranes.

Biochim Biophys Acta Biomembr 2019 Mar 16;1861(6):1057-1068. Epub 2019 Mar 16.

Department of Environmental Chemistry, Faculty of Chemistry, Jagiellonian University, Gronostajowa 2, 30-387 Kraków, Poland. Electronic address:

Polychlorinated biphenyls (PCB) are persistent organic pollutants that due to their chemical resistivity and inflammability found multiple applications. In spite of the global ban for PCB production, due to their long half-lives periods, PCB accumulate in the soils, so effective bioremediation of the polluted lands is of crucial importance. Some of the 209 PCB congeners exhibit increased toxicity to soil bacteria and their presence impoverish the soil decomposer community and slows down the degradation of environmental pollutants in the soils. Read More

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http://dx.doi.org/10.1016/j.bbamem.2019.03.009DOI Listing
March 2019
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Mercury leads to abnormal red blood cell adhesion to laminin mediated by membrane sulfatides.

Biochim Biophys Acta Biomembr 2019 Mar 16;1861(6):1162-1171. Epub 2019 Mar 16.

Case Biomanufacturing and Microfabrication Laboratory, Mechanical and Aerospace Engineering Department, Case Western Reserve University, Cleveland, OH, USA; Biomedical Engineering Department, Case Western Reserve University, Cleveland, OH, USA; Department of Orthopaedics, Case Western Reserve University, Cleveland, OH, USA. Electronic address:

Exposure to mercury is associated with numerous health problems, affecting different parts of the human body, including the nervous and cardiovascular systems in adults and children; however, the underlying mechanisms are yet to be fully elucidated. We investigated the role of membrane sulfatide on mercuric ion (Hg) mediated red blood cell (RBC) adhesion to a sub-endothelial matrix protein, laminin, using a microfluidic system that mimics microphysiological flow conditions. We exposed whole blood to mercury (HgCl), at a range of concentrations to mimic acute (high dose) and chronic (low dose) exposure, and examined RBC adhesion to immobilized laminin in microchannels at physiological flow conditions. Read More

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http://dx.doi.org/10.1016/j.bbamem.2019.03.008DOI Listing

Regulation of intracellular pH by electrogenic Na+/HCO3- co-transporters in embryonic neural stem cell-derived radial glia-like cells.

Biochim Biophys Acta Biomembr 2019 Mar 16;1861(6):1037-1048. Epub 2019 Mar 16.

Faculty of Medicine, Medicum, Division of Physiology, PO Box 63, University of Helsinki, FIN-00014 Helsinki, Finland.

A stroke causes a hypoxic brain microenvironment that alters neural cell metabolism resulting in cell membrane hyperpolarization and intracellular acidosis. We studied how intracellular pH (pH) is regulated in differentiated mouse neural progenitor cells during hyperpolarizing conditions, induced by prompt reduction of the extracellular K concentration. We found that the radial glia-like population in differentiating embryonic neural progenitor cells, but not neuronal cells, was rapidly acidified under these conditions. Read More

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http://dx.doi.org/10.1016/j.bbamem.2019.03.007DOI Listing
March 2019
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In vitro antileishmanial and cytotoxic activities of nerolidol are associated with changes in plasma membrane dynamics.

Biochim Biophys Acta Biomembr 2019 Mar 16;1861(6):1049-1056. Epub 2019 Mar 16.

Instituto de Física, Universidade Federal de Goiás, Goiânia, GO, Brazil. Electronic address:

The sesquiterpene nerolidol is a membrane-active compound that has demonstrated antitumor, antibacterial, antifungal and antiparasitic activities. In this study, we used electron paramagnetic resonance (EPR) spectroscopy and biophysical parameters determined via cell culture assays to study the mechanisms underlying the in vitro antileishmanial activity of nerolidol. The EPR spectra of a spin-labeled stearic acid indicated notable interactions of nerolidol with the cell membrane of Leishmania amazonensis amastigotes. Read More

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http://dx.doi.org/10.1016/j.bbamem.2019.03.006DOI Listing

Free energy analysis of membrane pore formation process in the presence of multiple melittin peptides.

Biochim Biophys Acta Biomembr 2019 Mar 15. Epub 2019 Mar 15.

Department of Materials Chemistry, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8603, Japan. Electronic address:

Understanding the molecular mechanism underlying pore formation in lipid membranes by antimicrobial peptides is of great importance in biological sciences as well as in drug design applications. Melittin has been widely studied as a pore forming peptide, though the molecular mechanism for pore formation is still illusive. We examined the free energy barrier for the creation of a pore in lipid membranes with and without multiple melittin peptides. Read More

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http://dx.doi.org/10.1016/j.bbamem.2019.03.002DOI Listing

Differential activity of lytic α-helical peptides on lactobacilli and lactobacilli-derived liposomes.

Biochim Biophys Acta Biomembr 2019 Mar 13;1861(6):1069-1077. Epub 2019 Mar 13.

Centro de Investigación y Desarrollo en Criotecnología de Alimentos (CIDCA,-CCT-CONICET La Plata, CICPBA, UNLP), RA-1900, Argentina; Cátedra de Microbiología, Facultad de Ciencias Exactas (FCE), UNLP, La Plata, Argentina. Electronic address:

Eukaryotic antimicrobial peptides (AMPs) interact with plasma membrane of bacteria, fungi and eukaryotic parasites. Noteworthy, Lactobacillus delbrueckii subsp. lactis (CIDCA 133) and L. Read More

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http://dx.doi.org/10.1016/j.bbamem.2019.03.004DOI Listing

Calcium-induced transformation of cardiolipin nanodisks.

Biochim Biophys Acta Biomembr 2019 May 13;1861(5):1030-1036. Epub 2019 Mar 13.

Department of Biochemistry and Molecular Biology, University of Nevada, Reno, Reno, NV 89557, United States of America. Electronic address:

Miniature membranes comprised of tetramyristoylcardiolipin (CL) and apolipoprotein (apo) A-I, termed nanodisks (ND), are stable, aqueous soluble, reconstituted high density lipoproteins. When CL ND, but not dimyristoylphosphatidylcholine (PC) ND, were incubated with CaCl, a concentration dependent increase in sample turbidity occurred, consistent with CL undergoing a bilayer to non-bilayer transition. To assess the cation specificity of this reaction, CL ND were incubated with various mono- and divalent cations. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00052736193005
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http://dx.doi.org/10.1016/j.bbamem.2019.03.005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6436997PMC
May 2019
3 Reads

Characterization of conjugated and unconjugated bile acid transport via human organic solute transporter α/β.

Biochim Biophys Acta Biomembr 2019 May 7;1861(5):1023-1029. Epub 2019 Mar 7.

Graduate School of Pharmaceutical Sciences, Tohoku University, 1-1 Seiryo-machi, Aoba-ku, Sendai 980-8574, Japan; Department of Pharmaceutical Sciences, Tohoku University Hospital, 1-1 Seiryo-machi, Aoba-ku, Sendai 980-8574, Japan.

Bile acids are biosynthesized from cholesterol in hepatocytes and usually localize in the enterohepatic circulation system. This system is regulated by several transporters that are expressed in the liver and intestine. Organic solute transporter (OST) α/β, which is known as a bidirectional transporter for some organic anions, contributes to the transport of bile acids; however, the transport properties of individual bile acids are not well understood. Read More

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http://dx.doi.org/10.1016/j.bbamem.2019.03.003DOI Listing

Efficient function of signal peptidase 1 of Escherichia coli is partly determined by residues in the mature N-terminus of exported proteins.

Biochim Biophys Acta Biomembr 2019 May 5;1861(5):1018-1022. Epub 2019 Mar 5.

Institute for Glycomics, Griffith University, Gold Coast Campus, Gold Coast, QLD 4222, Australia. Electronic address:

Exported proteins require an N-terminal signal peptide to direct them from the cytoplasm to the periplasm. Once the protein has been translocated across the cytoplasmic membrane, the signal peptide is cleaved by a signal peptidase, allowing the remainder of the protein to fold into its mature state in the periplasm. Signal peptidase I (LepB) cleaves non-lipoproteins and recognises the sequence Ala-X-Ala. Read More

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http://dx.doi.org/10.1016/j.bbamem.2019.03.001DOI Listing
May 2019
1 Read

Cardiolipin and phosphatidylethanolamine role in dibucaine interaction with the mitochondrial membrane.

Biochim Biophys Acta Biomembr 2019 Mar 3;1861(6):1152-1161. Epub 2019 Mar 3.

LAQV@Requimte, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre, 4169-007 Porto, Portugal. Electronic address:

Mitochondrial membranes are pointed out as the site of cardiotoxic action of local anaesthetics. Its three main phospholipids components are phosphatidylcholine, phosphatidylethanolamine and cardiolipin. Cardiolipins, in eukaryotes, are only found in mitochondria and are essential for the maintenance of its integrity and dynamics. Read More

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http://dx.doi.org/10.1016/j.bbamem.2019.02.011DOI Listing

Live-cell imaging of membrane proteins by a coiled-coil labeling method-Principles and applications.

Biochim Biophys Acta Biomembr 2019 May 1;1861(5):1011-1017. Epub 2019 Mar 1.

Graduate School of Pharmaceutical Sciences, Kyoto University, 46-29 Yoshida-Shimoadachi-cho, Sakyo-ku, Kyoto 606-8501, Japan. Electronic address:

In situ investigations in living cell membranes are important to elucidate the dynamic behaviors of membrane proteins in complex biomembrane environments. Protein-specific labeling is a key technique for the detection of a target protein by fluorescence imaging. The use of post-translational labeling methods using a genetically encodable tag and synthetic probes targeting the tag offer a smaller label size, labeling with synthetic fluorophores, and precise control of the labeling ratio in multicolor labeling compared with conventional genetic fusions with fluorescent proteins. Read More

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http://dx.doi.org/10.1016/j.bbamem.2019.02.009DOI Listing

Insights into water accessible pathways and the inactivation mechanism of proton translocation by the membrane-embedded domain of V-type ATPases.

Biochim Biophys Acta Biomembr 2019 May 1;1861(5):1004-1010. Epub 2019 Mar 1.

Bioinformatics Institute, Agency for Science, Technology and Research (A*STAR), 30 Biopolis Str., #07-01 Matrix, 138671, Singapore; National University of Singapore, Department of Biological Sciences, 14 Science Drive 4, 117543, Singapore. Electronic address:

V-type ATPases are multi-protein complexes, which acidify cellular compartments in eukaryotes. They pump protons against an ion gradient, driven by a mechano-chemical framework that exploits ATP hydrolysis as an energy source. This process drives the rotation of the so-called c-ring, a membrane embedded complex in the V-domain of the V-type ATPase, resulting in translocation of protons across the membrane. Read More

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http://dx.doi.org/10.1016/j.bbamem.2019.02.010DOI Listing

Interactions between RAMP2 and CRF receptors: The effect of receptor subtypes, splice variants and cell context.

Biochim Biophys Acta Biomembr 2019 May 28;1861(5):997-1003. Epub 2019 Feb 28.

Life and Health Sciences, Aston University, Birmingham B4 7ET, UK. Electronic address:

Corticotrophin releasing factor (CRF) acts via two family B G-protein-coupled receptors, CRFR1 and CRFR2. Additional subtypes exist due to alternative splicing. CRFR1α is the most widely expressed subtype and lacks a 29-residue insert in the first intracellular loop that is present in CRFR1β. Read More

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http://dx.doi.org/10.1016/j.bbamem.2019.02.008DOI Listing
May 2019
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Evolution and a revised nomenclature of P4 ATPases, a eukaryotic family of lipid flippases.

Biochim Biophys Acta Biomembr 2019 Feb 23;1861(6):1135-1151. Epub 2019 Feb 23.

Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark. Electronic address:

In all eukaryotic cells, P4 ATPases, also named phospholipid flippases, generate phospholipid asymmetry across biological membranes. This process is essential for cell survival, as it is required for vesicle budding and fusion in the secretory pathway. Several P4 ATPase isoforms can be identified in all sequenced eukaryotic genomes, but their evolution and interrelationships are poorly described. Read More

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http://dx.doi.org/10.1016/j.bbamem.2019.02.006DOI Listing
February 2019

Cooperativity and allostery in aquaporin 0 regulation by Ca.

Biochim Biophys Acta Biomembr 2019 May 22;1861(5):988-996. Epub 2019 Feb 22.

Department of Chemistry, University of California, Irvine, Irvine, CA 92697, United States of America. Electronic address:

Aquaporin 0 (AQP0) is essential for eye lens homeostasis as is regulation of its water permeability by Ca, which occurs through interactions with calmodulin (CaM), but the underlying molecular mechanisms are not well understood. Here, we use molecular dynamics (MD) simulations on the microsecond timescale under an osmotic gradient to explicitly model water permeation through the AQP0 channel. To identify any structural features that are specific to water permeation through AQP0, we also performed simulations of aquaporin 1 (AQP1) and a pure mixed lipid bilayer under the same conditions. Read More

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http://dx.doi.org/10.1016/j.bbamem.2019.02.007DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6437002PMC

Rsp5-dependent endocytosis and degradation of the arsenite transporter Acr3 requires its N-terminal acidic tail as an endocytic sorting signal and arrestin-related ubiquitin-ligase adaptors.

Biochim Biophys Acta Biomembr 2019 May 15;1861(5):916-925. Epub 2019 Feb 15.

Institute of Experimental Biology, University of Wroclaw, 50-328 Wroclaw, Poland. Electronic address:

The yeast plasma membrane transporter Acr3 mediates efflux of toxic arsenite and antimonite. Here, we investigated the mechanisms of Acr3 turnover. We found that after arrival and residence at the plasma membrane, Acr3 is subjected to internalization followed by proteolysis in the vacuole. Read More

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http://dx.doi.org/10.1016/j.bbamem.2019.02.004DOI Listing
May 2019
1 Read

Assemblies of lauryl maltose neopentyl glycol (LMNG) and LMNG-solubilized membrane proteins.

Biochim Biophys Acta Biomembr 2019 May 15;1861(5):939-957. Epub 2019 Feb 15.

Univ. Grenoble Alpes, CNRS, CEA, Institute for Structural Biology (IBS), 38000 Grenoble, France. Electronic address:

Laurylmaltose neopentylglycol (LMNG) bears two linked hydrophobic chains of equal length and two hydrophilic maltoside groups. It arouses a strong interest in the field of membrane protein biochemistry, since it was shown to efficiently solubilize and stabilize membrane proteins often better than the commonly used dodecylmaltopyranoside (DDM), and to allow structure determination of some challenging membrane proteins. However, LMNG was described to form large micelles, which could be unfavorable for structural purposes. Read More

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http://dx.doi.org/10.1016/j.bbamem.2019.02.003DOI Listing
May 2019
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3.836 Impact Factor

Binding of a Naja naja venom acidic phospholipase A cognate complex to membrane-bound vimentin of rat L6 cells: Implications in cobra venom-induced cytotoxicity.

Biochim Biophys Acta Biomembr 2019 May 15;1861(5):958-977. Epub 2019 Feb 15.

Microbial Biotechnology and Protein Research Laboratory, Department of Molecular Biology and Biotechnology, Tezpur University, Tezpur 784028, Assam, India. Electronic address:

An acidic phospholipase A enzyme (NnPLA-I) interacts with three finger toxins (cytotoxin and neurotoxin) from Naja naja venom to form cognate complexes to enhance its cytotoxicity towards rat L6 myogenic cells. The cytotoxicity was further enhanced in presence of trace quantity of venom nerve growth factor. The purified rat myoblast cell membrane protein showing interaction with NnPLA-I was identified as vimentin by LC-MS/MS analysis. Read More

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http://dx.doi.org/10.1016/j.bbamem.2019.02.002DOI Listing
May 2019
3.836 Impact Factor

Structure determination of UL49.5 transmembrane protein from bovine herpesvirus 1 by NMR spectroscopy and molecular dynamics.

Biochim Biophys Acta Biomembr 2019 May 14;1861(5):926-938. Epub 2019 Feb 14.

Faculty of Chemistry, University of Gdańsk, Wita Stwosza 63, 80-308 Gdańsk, Poland. Electronic address:

The transporter associated with antigen processing (TAP) directly participates in the immune response as a key component of the cytosolic peptide to major histocompatibility complex (MHC) class I protein loading machinery. This makes TAP an important target for viruses avoiding recognition by CD8+ T lymphocytes. Its activity can be suppressed by the UL49. Read More

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http://dx.doi.org/10.1016/j.bbamem.2019.02.005DOI Listing
May 2019
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Variations in carotenoid content and acyl chain composition in exponential, stationary and biofilm states of Staphylococcus aureus, and their influence on membrane biophysical properties.

Biochim Biophys Acta Biomembr 2019 May 13;1861(5):978-987. Epub 2019 Feb 13.

Department of Physics, Universidad de los Andes, Bogotá, Colombia. Electronic address:

Bacteria are often found in close association with surfaces, resulting in the formation of biofilms. In Staphylococcus aureus (S. aureus), biofilms are implicated in the resilience of chronic infections, presenting a serious clinical problem world-wide. Read More

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http://dx.doi.org/10.1016/j.bbamem.2019.02.001DOI Listing
May 2019
2 Reads

Interaction of salt with ether- and ester-linked phospholipid bilayers.

Biochim Biophys Acta Biomembr 2019 May 8;1861(5):907-915. Epub 2019 Feb 8.

Department of Physics, University of South Florida, Tampa, FL 33620, United States of America. Electronic address:

A distinguishing feature of Archaeal plasma membranes is that their phospholipids contain ether-links, as opposed to bacterial and eukaryotic plasma membranes where phospholipids primarily contain ester-links. Experiments show that this chemical difference in headgroup-tail linkage does produce distinct differences in model bilayer properties. Here we examine the effects of salt on bilayer structure in the case of an ether-linked lipid bilayer. Read More

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http://dx.doi.org/10.1016/j.bbamem.2019.01.016DOI Listing

Temperature dependent self-organization of DMPC membranes promoted by intermediate amounts of the saponin aescin.

Biochim Biophys Acta Biomembr 2019 May 5;1861(5):897-906. Epub 2019 Feb 5.

Physical and Biophysical Chemistry, Bielefeld University, Universitätsstr. 25, 33615 Bielefeld, Germany. Electronic address:

The plant-derived biosurfactant aescin is naturally present in many plants and is used for treatment of disorders such as varicose veins and inflammation of veins. The hemolytic activity of this saponin is attributed to its interaction with cholesterol in the red blood cell membrane. This work investigates the phase and aggregation behavior of saponin-containing model membranes consisting of the phospholipid 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC). Read More

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http://dx.doi.org/10.1016/j.bbamem.2019.01.015DOI Listing
May 2019
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Labelling strategy and membrane characterization of marine bacteria Vibrio splendidus by in vivoH NMR.

Biochim Biophys Acta Biomembr 2019 Apr 2;1861(4):871-878. Epub 2019 Feb 2.

Department of Chemistry, Université du Québec à Montréal, P.O. Box 8888, Downtown Station, Montreal H3C 3P8, Canada. Electronic address:

Vibrio splendidus is a marine bacterium often considered as a threat in aquaculture hatcheries where it is responsible for mass mortality events, notably of bivalves' larvae. This bacterium is highly adapted to dynamic salty ecosystems where it has become an opportunistic and resistant species. To characterize their membranes as a first and necessary step toward studying bacterial interactions with diverse molecules, we established a labelling protocol for in vivoH solid-state nuclear magnetic resonance (SS-NMR) analysis of V. Read More

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http://dx.doi.org/10.1016/j.bbamem.2019.01.018DOI Listing

Influence of 7α-hydroxycholesterol on sphingomyelin and sphingomyelin/phosphatidylcholine films - The Langmuir monolayer study complemented with theoretical calculations.

Biochim Biophys Acta Biomembr 2019 Apr 1;1861(4):861-870. Epub 2019 Feb 1.

Jagiellonian University, Faculty of Chemistry, Gronostajowa 2, 30-387 Kraków, Poland. Electronic address:

Under pathological conditions, cholesterol oxidation products (oxysterols) appear in enhanced concentration in blood and cerebrospinal fluid, which leads to cytotoxic effect, especially in central nervous system. However, the mode of action of oxysterols on the membrane level has not been fully resolved. In this paper we have investigated the interaction between 7α- hydroxycholesterol, 7α-OH (one of the most abundant oxysterol in human body) and two major membrane lipids: sphingomyelin, SM (basic component of lipid rafts and nerve membrane) and 1-palmitoyl-2-oleoyl-glycero-3-phosphocholine, POPC (main phospholipid of mammalian cell membranes). Read More

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http://dx.doi.org/10.1016/j.bbamem.2019.01.020DOI Listing
April 2019
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Oxygen distribution in the fluid/gel phases of lipid membranes.

Biochim Biophys Acta Biomembr 2019 Apr 1;1861(4):879-886. Epub 2019 Feb 1.

Institut für Physik, Humboldt-Universität zu Berlin, Newtonstraße 15, 12489 Berlin, Germany. Electronic address:

The interactions between oxygen and lipid membranes play fundamental roles in basic biological processes (e.g., cellular respiration). Read More

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http://dx.doi.org/10.1016/j.bbamem.2019.01.019DOI Listing

Selection and redesign for high selectivity of membrane-active antimicrobial peptides from a dedicated sequence/function database.

Biochim Biophys Acta Biomembr 2019 Apr 30;1861(4):827-834. Epub 2019 Jan 30.

Department of Life Sciences, University of Trieste, Trieste, Italy. Electronic address:

Antimicrobial peptides (AMPs) are plausible candidates for the development of novel classes of antibiotics with a low tendency to elicit resistance. They often form lesions in the bacterial membrane making it hard for bacteria to develop permanent resistance. However, a potent antibacterial activity is often accompanied by excessive cytotoxicity towards host cells. Read More

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http://dx.doi.org/10.1016/j.bbamem.2019.01.017DOI Listing
April 2019
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Minimal nanodisc without exogenous lipids for stabilizing membrane proteins in detergent-free buffer.

Biochim Biophys Acta Biomembr 2019 Apr 30;1861(4):852-860. Epub 2019 Jan 30.

Univ. Bordeaux, CBMN UMR 5248, Bordeaux INP, F-33600 Pessac, France; CNRS, CBMN UMR5248, F-33600 Pessac, France. Electronic address:

Membrane protein stabilization after detergent solubilization presents drawbacks for structural and biophysical studies, in particular that of a reduced stability in detergent micelles. Therefore, alternative methods are required for efficient stabilization. Lipid nanodisc made with the membrane scaffold protein MSP is a valuable system but requires a fine optimization of the lipid to protein ratio. Read More

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http://dx.doi.org/10.1016/j.bbamem.2019.01.013DOI Listing
April 2019
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Interaction of the cholesterol reducing agent simvastatin with zwitterionic DPPC and charged DPPG phospholipid membranes.

Biochim Biophys Acta Biomembr 2019 Apr 29;1861(4):810-818. Epub 2019 Jan 29.

Middle East Technical University, Department of Biological Science, Ankara, Turkey; Altınbaş University, Medical School, Department of Biophysics, Istanbul, Turkey; Altınbas University, Biomedical Sciences Graduate Programme, Institute of Health, Istanbul, Turkey. Electronic address:

Simvastatin is a lipid-lowering drug in the pharmaceutical group statins. Interaction of a drug with lipids may define its role in the system and be critical for its pharmacological activity. We examined the interactions of simvastatin with zwitterionic dipalmitoyl phosphatidylcholine (DPPC) and anionic dipalmitoyl phosphatidylglycerol (DPPG) multilamellar vesicles (MLVs) as a function of temperature at different simvastatin concentrations using Fourier transform infrared (FTIR) spectroscopy and differential scanning calorimetry (DSC). Read More

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http://dx.doi.org/10.1016/j.bbamem.2019.01.014DOI Listing

The effect of carotenoids on the concentration of singlet oxygen in lipid membranes.

Biochim Biophys Acta Biomembr 2019 Apr 26;1861(4):845-851. Epub 2019 Jan 26.

Department of Biophysics, Institute of Physics, Maria Curie-Sklodowska University, Lublin, Poland.

An effect of β-carotene and its polar derivative, zeaxanthin, on a concentration of singlet oxygen in lipid membranes was studied in a model system. The carotenoids were incorporated into the membranes of small unilamellar liposomes at a concentration of 0.15 mol% with respect to lipid. Read More

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http://dx.doi.org/10.1016/j.bbamem.2019.01.012DOI Listing

Role of non-electrostatic forces in antimicrobial potency of a dengue-virus derived fusion peptide VG16KRKP: Mechanistic insight into the interfacial peptide-lipid interactions.

Biochim Biophys Acta Biomembr 2019 Apr 25;1861(4):798-809. Epub 2019 Jan 25.

Department of Biophysics, Bose Institute, P-1/12 CIT Scheme VII(M), Kolkata 700054, India. Electronic address:

Cationic antimicrobial peptides (AMPs) are emerging as effective alternatives to conventional therapeutics that are used against the ever-rising number of multidrug-resistant microbial strains. Most studies established the peptide's amphipathicity and electrostatic interaction with the membrane as the basis for their antimicrobial effect. However, the interplay between the stoichiometric ratio of lipids, local geometry, diverse physicochemical properties of the host membranes and antimicrobial peptide efficacy is still poorly understood. Read More

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http://dx.doi.org/10.1016/j.bbamem.2019.01.011DOI Listing
April 2019
3.836 Impact Factor

Exploring electrostatic interactions of relaxin family peptide receptor 3 and 4 with ligands using a NanoBiT-based binding assay.

Biochim Biophys Acta Biomembr 2019 Apr 24;1861(4):776-786. Epub 2019 Jan 24.

Research Center for Translational Medicine at East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai, China. Electronic address:

Relaxin family peptides perform a variety of biological functions by activating four G protein-coupled receptors, namely relaxin family peptide receptor 1-4 (RXFP1-4). We recently disclosed electrostatic interactions of the homologous RXFP3 and RXFP4 with some agonists based on activation complementation. However, this activation assay-based approach cannot be applied to antagonists that do not activate receptors. Read More

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http://dx.doi.org/10.1016/j.bbamem.2019.01.010DOI Listing
April 2019
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Sterol structure dependence of insulin receptor and insulin-like growth factor 1 receptor activation.

Biochim Biophys Acta Biomembr 2019 Apr 22;1861(4):819-826. Epub 2019 Jan 22.

Department of Physiology and Biophysics, Stony Brook University, Stony Brook, NY 11794-8661, United States of America; Department of Veterans Affairs Medical Center, Northport, NY 11768, United States of America. Electronic address:

The plasma membrane is a dynamic environment with a complex composition of lipids, proteins, and cholesterol. Areas enriched in cholesterol and sphingolipids are believed to form lipid rafts, domains of highly ordered lipids. The unique physical properties of these domains have been proposed to influence many cellular processes. Read More

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http://dx.doi.org/10.1016/j.bbamem.2019.01.009DOI Listing

Boundary lipids of the nicotinic acetylcholine receptor: Spontaneous partitioning via coarse-grained molecular dynamics simulation.

Biochim Biophys Acta Biomembr 2019 Apr 18;1861(4):887-896. Epub 2019 Jan 18.

Center for Computational and Integrative Biology, Rutgers University-Camden, Camden, NJ, United States of America; Department of Physics, Rutgers University-Camden, Camden, NJ, United States of America. Electronic address:

Reconstituted nicotinic acetylcholine receptors (nAChRs) exhibit significant gain-of-function upon addition of cholesterol to reconstitution mixtures, and cholesterol affects the organization of nAChRs within domain-forming membranes, but whether nAChR partitions to cholesterol-rich liquid-ordered ("raft" or l) domains or cholesterol-poor liquid-disordered (l) domains is unknown. We use coarse-grained molecular dynamics simulations to observe spontaneous interactions of cholesterol, saturated lipids, and polyunsaturated (PUFA) lipids with nAChRs. In binary Dipalmitoylphosphatidylcholine:Cholesterol (DPPC:CHOL) mixtures, both CHOL and DPPC acyl chains were observed spontaneously entering deep "non-annular" cavities in the nAChR TMD, particularly at the subunit interface and the β subunit center, facilitated by the low amino acid density in the cryo-EM structure of nAChR in a native membrane. Read More

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http://dx.doi.org/10.1016/j.bbamem.2019.01.005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6392067PMC
April 2019
2 Reads

Single-channel permeability and glycerol affinity of human aquaglyceroporin AQP3.

Biochim Biophys Acta Biomembr 2019 Apr 17;1861(4):768-775. Epub 2019 Jan 17.

Department of Biology and Neurosciences Institute, University of Texas at San Antonio, San Antonio, TX 78249, United States of America.

For its fundamental relevance, transport of water and glycerol across the erythrocyte membrane has long been investigated before and after the discovery of aquaporins (AQPs), the membrane proteins responsible for water and glycerol transport. AQP1 is abundantly expressed in the human erythrocyte for maintaining its hydrohomeostasis where AQP3 is also expressed (at a level ~30-folds lower than AQP1) facilitating glycerol transport. This research is focused on two of the remaining questions: How permeable is AQP3 to water? What is the glycerol-AQP3 affinity under near-physiological conditions? Through atomistic modelling and large-scale simulations, we found that AQP3 is two to three times more permeable to water than AQP1 and that the glycerol-AQP3 affinity is approximately 500/M. Read More

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http://dx.doi.org/10.1016/j.bbamem.2019.01.008DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6382548PMC
April 2019
2 Reads