98,091 results match your criteria Biochim. Biophys. Acta[Journal]


The importance of being (slightly) modified: The role of rRNA editing on gene expression control and its connections with cancer.

Biochim Biophys Acta 2016 12 2;1866(2):330-338. Epub 2016 Nov 2.

Department of Experimental, Diagnostic and Specialty Medicine, University of Bologna, Via Massarenti 9, 40138 Bologna, Italy. Electronic address:

In human ribosomal RNAs, over 200 residues are modified by specific, RNA-driven enzymatic complexes or stand-alone, RNA-independent enzymes. In most cases, modification sites are placed in specific positions within important functional areas of the ribosome. Some evidence indicates that the altered control in ribosomal RNA modifications may affect ribosomal function during mRNA translation. Read More

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http://dx.doi.org/10.1016/j.bbcan.2016.10.007DOI Listing
December 2016

Yersinia YopJ negatively regulates IRF3-mediated antibacterial response through disruption of STING-mediated cytosolic DNA signaling.

Biochim Biophys Acta 2016 12 11;1863(12):3148-3159. Epub 2016 Oct 11.

Key Laboratory for Molecular Enzymology & Engineering, the Ministry of Education, Jilin University, Changchun 130012, China; College of Life Science, Jilin University, Changchun 130012, China. Electronic address:

The Yersinia outer protein J (YopJ) plays a pivotal role in evading the host immune response and establishes a persistent infection in host cells after bacterial infection. YopJ is a cysteine protease and can act as a deubiquitinating enzyme that deubiquitinates several targets in multiple signaling pathways. Stimulator of interferon genes (STING) is a critical adapter for the induction of interferon regulatory factor 3 (IRF3) phosphorylation and subsequent production of the cytokines in response to nucleic acids in the cytoplasm. Read More

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http://dx.doi.org/10.1016/j.bbamcr.2016.10.004DOI Listing
December 2016
18 Reads

SAGA complex and Gcn5 are necessary for respiration in budding yeast.

Biochim Biophys Acta 2016 12 11;1863(12):3160-3168. Epub 2016 Oct 11.

Institute of Molecular Biology and Pathology-CNR, Sapienza University of Rome, P.le A. Moro 5, Rome, Italy. Electronic address:

In budding yeast, growth through fermentation and/or respiration is dependent on the type of carbon source present in the medium. SAGA complex is the main acetylation complex and is required, together with Rtg factors, for nucleus-mitochondria communication and transcriptional activation of specific nuclear genes. Even though acetylation is necessary for mitochondria activity and respiratory pathways the direct role of histone acetyltransferases and SAGA complex has never been investigated directly. Read More

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http://dx.doi.org/10.1016/j.bbamcr.2016.10.002DOI Listing
December 2016
1 Read

Nuclear PTEN interferes with binding of Ku70 at double-strand breaks through post-translational poly(ADP-ribosyl)ation.

Biochim Biophys Acta 2016 12 11;1863(12):3106-3115. Epub 2016 Oct 11.

Department of Biotechnology, College of Basic Medical Sciences, Dalian Medical University, Dalian 116044, China. Electronic address:

PTEN is a tumor suppressor gene characterized as a phosphatase that antagonizes the phosphatidylinositol 3-kinase signaling pathway in the cytoplasm. Nuclear PTEN plays roles in chromosomal stability, in which the double-strand breaks (DSB) repair mediated by homologous recombination (HR) and non-homologous end joining (NHEJ) is critical. Herein, the role of nuclear PTEN in DSB repair and the underlying molecular mechanism was investigated in this study. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01674889163025
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http://dx.doi.org/10.1016/j.bbamcr.2016.10.003DOI Listing
December 2016
7 Reads

Inorganic cadmium affects the fluidity and size of phospholipid based liposomes.

Biochim Biophys Acta 2016 12 11;1858(12):3169-3181. Epub 2016 Oct 11.

Department of Biological Sciences, University of Calgary, Calgary, Alberta, T2N 1N4, Canada. Electronic address:

Following intake and absorption of Cd into the bloodstream, one possible target is the lipid membrane surrounding erythrocytes as well as kidney and liver cells where Cd accumulates. We investigated the interactions of Cd with model membranes from a biophysical perspective by using fluorescence spectroscopy and dynamic light scattering to monitor changes in liposome size, membrane fluidity and lipid phase transition. The fluorescent probe Laurdan was incorporated into liposomes and used to quantitate cadmium induced fluidity changes in model systems hydrated in 20mM HEPES, 100mM NaCl pH7. Read More

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http://dx.doi.org/10.1016/j.bbamem.2016.10.005DOI Listing
December 2016
8 Reads

Mechanical strain stimulates vasculogenesis and expression of angiogenesis guidance molecules of embryonic stem cells through elevation of intracellular calcium, reactive oxygen species and nitric oxide generation.

Biochim Biophys Acta 2016 12 8;1863(12):3096-3105. Epub 2016 Oct 8.

Department of Physiology, Faculty of Medicine, Justus Liebig University Giessen, Germany. Electronic address:

Objectives: Differentiation of embryonic stem (ES) cells may be regulated by mechanical strain. Herein, signaling molecules underlying mechanical stimulation of vasculogenesis and expression of angiogenesis guidance cues were investigated in ES cell-derived embryoid bodies.

Methods And Results: Treatment of embryoid bodies with 10% static mechanical strain using a Flexercell strain system significantly increased CD31-positive vascular structures and the angiogenesis guidance molecules plexinB1, ephrin B2, neuropilin1 (NRP1), semaphorin 4D (sem4D) and robo4 as well as vascular endothelial growth factor (VEGF), fibroblast growth factor-2 (FGF-2) and platelet-derived growth factor-BB (PDGF-BB) as evaluated by Western blot and real time RT-PCR. Read More

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http://dx.doi.org/10.1016/j.bbamcr.2016.10.001DOI Listing
December 2016
21 Reads

Ligand binding affinity and changes in the lateral diffusion of receptor for advanced glycation endproducts (RAGE).

Biochim Biophys Acta 2016 12 7;1858(12):3141-3149. Epub 2016 Oct 7.

Department of Chemistry, Iowa State University, 1605 Gilman Hall, Ames, IA 50011, United States. Electronic address:

The effect of ligand on the lateral diffusion of receptor for advanced glycation endproducts (RAGE), a receptor involved in numerous pathological conditions, remains unknown. Single particle tracking experiments that use quantum dots specifically bound to hemagglutinin (HA)-tagged RAGE (HA-RAGE) are reported to elucidate the effect of ligand binding on HA-RAGE diffusion in GM07373 cell membranes. The ligand used in these studies is methylglyoxal modified-bovine serum albumin (MGO-BSA) containing advanced glycation end products modifications. Read More

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http://dx.doi.org/10.1016/j.bbamem.2016.10.001DOI Listing
December 2016
10 Reads

Role of pannexin 1 in Clostridium perfringens beta-toxin-caused cell death.

Biochim Biophys Acta 2016 12 5;1858(12):3150-3156. Epub 2016 Oct 5.

Department of Microbiology, Faculty of Pharmaceutical Sciences, Tokushima Bunri University, Yamashiro-cho 180, Tokushima 770-8514, Japan. Electronic address:

Background: Beta-toxin produced by Clostridium perfringens is a key virulence factor of fatal hemorrhagic enterocolitis and enterotoxemia. This toxin belongs to a family of β-pore-forming toxins (PFTs). We reported recently that the ATP-gated P2X receptor interacts with beta-toxin. Read More

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http://dx.doi.org/10.1016/j.bbamem.2016.10.003DOI Listing
December 2016
6 Reads

Coarse-grained simulations of hemolytic peptide δ-lysin interacting with a POPC bilayer.

Biochim Biophys Acta 2016 12 6;1858(12):3182-3194. Epub 2016 Oct 6.

Department of Chemistry and Biochemistry, University of North Carolina Wilmington, Wilmington, NC 28403, United States. Electronic address:

δ-lysin, secreted by a Gram-positive bacterium Staphylococcus aureus, is a 26-residue membrane active peptide that shares many common features with antimicrobial peptides (AMPs). However, it possesses a few unique features that differentiate itself from typical AMPs. In particular, δ-lysin has zero net charge, even though it has many charged residues, and it preferentially lyses eukaryotic cells over bacterial cells. Read More

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http://dx.doi.org/10.1016/j.bbamem.2016.10.004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5096981PMC
December 2016
14 Reads

Eicosapentaenoic acid reduces membrane fluidity, inhibits cholesterol domain formation, and normalizes bilayer width in atherosclerotic-like model membranes.

Biochim Biophys Acta 2016 12 5;1858(12):3131-3140. Epub 2016 Oct 5.

CSIR-Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India.

Cholesterol crystalline domains characterize atherosclerotic membranes, altering vascular signaling and function. Omega-3 fatty acids reduce membrane lipid peroxidation and subsequent cholesterol domain formation. We evaluated non-peroxidation-mediated effects of eicosapentaenoic acid (EPA), other TG-lowering agents, docosahexaenoic acid (DHA), and other long-chain fatty acids on membrane fluidity, bilayer width, and cholesterol domain formation in model membranes. Read More

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http://dx.doi.org/10.1016/j.bbamem.2016.10.002DOI Listing
December 2016
6 Reads

Cytoplasmic RNA decay pathways - Enzymes and mechanisms.

Biochim Biophys Acta 2016 12 3;1863(12):3125-3147. Epub 2016 Oct 3.

Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5A, 02-106 Warsaw, Poland; Institute of Genetics and Biotechnology, Faculty of Biology, University of Warsaw, Pawinskiego 5A, 02-106 Warsaw, Poland. Electronic address:

RNA decay plays a crucial role in post-transcriptional regulation of gene expression. Work conducted over the last decades has defined the major mRNA decay pathways, as well as enzymes and their cofactors responsible for these processes. In contrast, our knowledge of the mechanisms of degradation of non-protein coding RNA species is more fragmentary. Read More

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http://dx.doi.org/10.1016/j.bbamcr.2016.09.023DOI Listing
December 2016
4 Reads

Regulation of Mitofusin1 by Mahogunin Ring Finger-1 and the proteasome modulates mitochondrial fusion.

Biochim Biophys Acta 2016 12 4;1863(12):3065-3083. Epub 2016 Oct 4.

Biophysics & Structural Genomics Division, Saha Institute of Nuclear Physics, 1/AF Bidhannagar, Kolkata, 700064, India. Electronic address:

Health and homoeostasis are maintained by a dynamic balance between mitochondrial fission and fusion. Mitochondrial fusion machinery is largely unknown in mammals. Only a few reports have illustrated the role of Fzo1 in mitochondrial fusion known in Saccharomyces cerevisiae. Read More

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http://dx.doi.org/10.1016/j.bbamcr.2016.09.022DOI Listing
December 2016
1 Read
3 Citations

Corrigendum to "The transport mechanism of the mitochondrial ADP/ATP carrier" [Biochim. Biophys. Acta 1863/10 (2016) 2379-2393].

Biochim Biophys Acta 2016 12 6;1863(12):3169. Epub 2016 Oct 6.

The Medical Research Council, Mitochondrial Biology Unit, Cambridge Biomedical Campus, Wellcome Trust/MRC Building, Hills Road, Cambridge CB2 0XY, UK.

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http://dx.doi.org/10.1016/j.bbamcr.2016.09.021DOI Listing
December 2016
9 Reads

Cardiac ankyrin repeat protein attenuates cardiomyocyte apoptosis by upregulation of Bcl-2 expression.

Biochim Biophys Acta 2016 12 3;1863(12):3040-3049. Epub 2016 Oct 3.

Department of Cardiology, Cardiovascular Key Lab of Zhejiang Province, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310009, PR China. Electronic address:

Cardiac ankyrin repeat protein (CARP) is a nuclear transcriptional co-factor that has additional functions in the myoplasm as a component of the muscle sarcomere. Previous studies have demonstrated increased expression of CARP in cardiovascular diseases, however, its role in cardiomyocyte apoptosis is unclear and controversial. In the present study, we investigated possible roles of CARP in hypoxia/reoxygenation (H/R) -induced cardiomyocyte apoptosis and the underlying mechanisms. Read More

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http://dx.doi.org/10.1016/j.bbamcr.2016.09.024DOI Listing
December 2016
43 Reads

Differential subcellular distribution of four phospholipase C isoforms and secretion of GPI-PLC activity.

Biochim Biophys Acta 2016 12 29;1858(12):3157-3168. Epub 2016 Sep 29.

Saarland University, Molecular Cell Dynamics, Centre for Human and Molecular Biology, Campus A2 4, 66123 Saarbrücken, Germany. Electronic address:

Phospholipase C (PLC) is an important enzyme of signal transduction pathways by generation of second messengers from membrane lipids. PLCs are also indicated to cleave glycosylphosphatidylinositol (GPI)-anchors of surface proteins thus releasing these into the environment. However, it remains unknown whether this enzymatic activity on the surface is due to distinct PLC isoforms in higher eukaryotes. Read More

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http://dx.doi.org/10.1016/j.bbamem.2016.09.022DOI Listing
December 2016
7 Reads

Inhibition of CDK7 bypasses spindle assembly checkpoint via premature cyclin B degradation during oocyte meiosis.

Biochim Biophys Acta 2016 12;1863(12):2993-3000

Department of Animal Sciences, Chungbuk National University, Cheongju, Republic of Korea. Electronic address:

To ensure accurate chromosome segregation, the spindle assembly checkpoint (SAC) delays anaphase onset by preventing the premature activation of anaphase-promoting complex/cyclosome (APC/C) until all kinetochores are attached to the spindle. Although an escape from mitosis in the presence of unsatisfied SAC has been shown in several cancer cells, it has not been reported in oocyte meiosis. Here, we show that CDK7 activity is required to prevent a bypass of SAC during meiosis I in mouse oocytes. Read More

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http://dx.doi.org/10.1016/j.bbamcr.2016.09.020DOI Listing
December 2016
11 Reads

Mastoparan is a membranolytic anti-cancer peptide that works synergistically with gemcitabine in a mouse model of mammary carcinoma.

Biochim Biophys Acta 2016 12 18;1858(12):3195-3204. Epub 2016 Oct 18.

University of British Columbia, Vancouver, BC, Canada. Electronic address:

Anti-cancer peptides (ACPs) are small cationic and hydrophobic peptides that are more toxic to cancer cells than normal cells. ACPs kill cancer cells by causing irreparable membrane damage and cell lysis, or by inducing apoptosis. Direct-acting ACPs do not bind to a unique receptor, but are rather attracted to several different molecules on the surface of cancer cells. Read More

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http://dx.doi.org/10.1016/j.bbamem.2016.09.021DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5097029PMC
December 2016
21 Reads

TonB-dependent ligand trapping in the BtuB transporter.

Biochim Biophys Acta 2016 12 6;1858(12):3105-3112. Epub 2016 Oct 6.

Department of Biochemistry & Molecular Biology, Life Sciences Institute, University of British Columbia, 2350 Health Sciences Mall, Vancouver, BC V6T 1Z3, Canada. Electronic address:

TonB-dependent transporters are β-barrel outer membrane proteins occluded by a plug domain. Upon ligand binding, these transporters extend a periplasmic motif termed the TonB box. The TonB box permits the recruitment of the inner membrane protein complex TonB-ExbB-ExbD, which drives import of ligands in the cell periplasm. Read More

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http://dx.doi.org/10.1016/j.bbamem.2016.09.019DOI Listing
December 2016

Altered regulation of the Spry2/Dyrk1A/PP2A triad by homocysteine impairs neural progenitor cell proliferation.

Biochim Biophys Acta 2016 12 26;1863(12):3015-3026. Epub 2016 Sep 26.

Área de Fisiología, Facultad de Medicina, Universidad de Cádiz, Spain. Electronic address:

Hyperhomocysteinemia reduces neurogenesis in the adult mouse brain. Homocysteine (Hcy) inhibits postnatal neural progenitor cell (NPC) proliferation by specifically impairing the fibroblast growth factor receptor (FGFR)-Erk1/2-cyclin E signaling pathway. We demonstrate herein that the inhibition of FGFR-dependent NPC proliferation induced by Hcy is mediated by its capacity to alter the cellular methylation potential. Read More

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http://dx.doi.org/10.1016/j.bbamcr.2016.09.018DOI Listing
December 2016
12 Reads

The poly(ADP-ribosyl)ation of FoxO3 mediated by PARP1 participates in isoproterenol-induced cardiac hypertrophy.

Biochim Biophys Acta 2016 12;1863(12):3027-3039

Department of Pharmacology and Toxicology, School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou 510006, Guangdong, PR China; National and Local United Engineering Lab of Druggability and New Drugs Evaluation, School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou 510006, Guangdong, PR China; Guangdong Provincial Key Laboratory of Construction Foundation, School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou 510006, Guangdong, PR China. Electronic address:

The Forkhead box-containing protein, O subfamily 3 (FoxO3) transcription factor negatively regulates myocardial hypertrophy, and its transcriptional activity is finely conditioned by diverse posttranslational modifications, such as phosphorylation, acetylation, ubiquitination, methylation and glycosylation. Here, we introduce a novel modification of the FoxO3 protein in cardiomyocytes: poly(ADP-ribosyl)ation (PARylation) mediated by poly(ADP-ribose) polymerase-1 (PARP1). This process catalyzes the NAD-dependent synthesis of polymers of ADP-ribose (PAR) and their subsequent attachment to target proteins by PARPs. Read More

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http://dx.doi.org/10.1016/j.bbamcr.2016.09.019DOI Listing
December 2016
13 Reads

Cullin9 protects mouse eggs from aneuploidy by controlling microtubule dynamics via Survivin.

Biochim Biophys Acta 2016 12 24;1863(12):2934-2941. Epub 2016 Sep 24.

College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. Electronic address:

The Cullin9 gene encodes a putative E3 ligase that serves a wide variety of biological functions in mitosis, whereas its roles in meiosis have not yet clearly defined. Here, we report that Cullin9 accumulates on the spindle apparatus and colocalizes with the microtubule fibers during mouse oocyte meiotic maturation. Depletion of Cullin9 by morpholino microinjection results in a remarkably higher rate of disorganized spindles and misaligned chromosomes in oocytes, which is coupled with the impaired kinetochore-microtubule attachments. Read More

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http://dx.doi.org/10.1016/j.bbamcr.2016.09.017DOI Listing
December 2016
3 Reads

Effects of flavonol glycosides on liposome stability during freezing and drying.

Biochim Biophys Acta 2016 12 24;1858(12):3050-3060. Epub 2016 Sep 24.

Max-Planck-Institut für Molekulare Pflanzenphysiologie, Am Mühlenberg 1, D-14476 Potsdam, Germany. Electronic address:

Flavonoids are a large and diverse group of plant secondary metabolites that are mainly present as glycosides. They are often accumulated in response to abiotic stresses such as UV radiation, drought, cold and freezing. The most extensively studied function of flavonoids is their antioxidant activity although their importance as antioxidants in plants has been questioned. Read More

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http://dx.doi.org/10.1016/j.bbamem.2016.09.020DOI Listing
December 2016
8 Reads

All-atom molecular dynamics simulations of lung surfactant protein B: Structural features of SP-B promote lipid reorganization.

Biochim Biophys Acta 2016 12 23;1858(12):3082-3092. Epub 2016 Sep 23.

Department of Physics and Physical Oceanography, Memorial University of Newfoundland, St. John's, NL A1B 3X7, Canada; Department of Biochemistry, Memorial University of Newfoundland, St. John's, NL A1B 3X9, Canada. Electronic address:

Lung surfactant protein B (SP-B), a 79 residue, hydrophobic protein from the saposin superfamily, plays an essential role in breathing. Because of the extreme hydrophobicity of SP-B, the experimental structure of this protein has not yet been determined. Here, we run all-atom molecular dynamics simulations using the OPLS-AA force field in GROMACS to study SP-B's structure and mechanisms for promoting lipid reorganization. Read More

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http://dx.doi.org/10.1016/j.bbamem.2016.09.018DOI Listing
December 2016
4 Reads

Biased signalling is an essential feature of TLR4 in glioma cells.

Biochim Biophys Acta 2016 12 23;1863(12):3084-3095. Epub 2016 Sep 23.

Stem Cell Biology and Regenerative Medicine, School of Pharmacy, University of Reading, Reading, United Kingdom. Electronic address:

A distinct feature of the Toll-like receptor 4 (TLR4) is its ability to trigger both MyD88-dependent and MyD88-independent signalling, culminating in activation of pro-inflammatory NF-κB and/or the antiviral IRF3. Although TLR4 agonists (lipopolysaccharides; LPSs) derived from different bacterial species have different endotoxic activity, the impact of LPS chemotype on the downstream signalling is not fully understood. Notably, different TLR4 agonists exhibit anti-tumoural activity in animal models of glioma, but the underlying molecular mechanisms are largely unknown. Read More

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http://dx.doi.org/10.1016/j.bbamcr.2016.09.016DOI Listing
December 2016
8 Reads

Transport of haloacids across biological membranes.

Biochim Biophys Acta 2016 12 23;1858(12):3061-3070. Epub 2016 Sep 23.

Molecular Microbiology Laboratory, School of Biological Sciences, The University of Hong Kong, Pokfulam Road, Hong Kong. Electronic address:

Haloacids are considered to be environmental pollutants, but some of them have also been tested in clinical research. The way that haloacids are transported across biological membranes is important for both biodegradation and drug delivery purposes. In this review, we will first summarize putative haloacids transporters and the information about haloacids transport when studying carboxylates transporters. Read More

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http://dx.doi.org/10.1016/j.bbamem.2016.09.017DOI Listing
December 2016
8 Reads

Inhibition of autophagy sensitises cells to hydrogen peroxide-induced apoptosis: Protective effect of mild thermotolerance acquired at 40°C.

Biochim Biophys Acta 2016 12 22;1863(12):3050-3064. Epub 2016 Sep 22.

Département des sciences biologiques (TOXEN, BIOMED), Université du Québec à Montréal, Montréal, Québec, Canada. Electronic address:

Various toxic compounds produce reactive oxygen species, resulting in oxidative stress that threatens cellular homeostasis. Yet, lower doses of stress can stimulate defence systems allowing cell survival, whereas intense stress activates cell death pathways such as apoptosis. Mild thermal stress (40°C, 3h) induces thermotolerance, an adaptive survival response that renders cells less sensitive to subsequent toxic stress, by activating defence systems like heat shock proteins, antioxidants, anti-apoptotic and ER-stress factors. Read More

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http://dx.doi.org/10.1016/j.bbamcr.2016.09.015DOI Listing
December 2016
1 Read

An extensive simulation study of lipid bilayer properties with different head groups, acyl chain lengths, and chain saturations.

Biochim Biophys Acta 2016 12 21;1858(12):3093-3104. Epub 2016 Sep 21.

Department of Chemical and Biomolecular Engineering, University of Maryland, College Park, MD 20742, USA; Biophysics Program, University of Maryland, College Park, MD 20742, USA. Electronic address:

Previous MD simulations of six phosphocholine (PC) lipid bilayers demonstrated the accuracy of the CHARMM36 force field (C36FF) for PC bilayer simulation at varied temperatures (BBA-Biomembranes, 1838 (2014): 2520-2529). In this work, we further examine the accuracy of C36FF over a wide temperature range for a broader range of lipid types such as various head groups (phosphatidic acid (PA), PC, phosphoethanolamine (PE), phosphoglycerol (PG), and phosphoserine (PS)), and tails (saturated, mono-, mixed- and poly-unsaturated acyl chains with varied chain lengths). The structural properties (surface area per lipid (SA/lip), overall bilayer thickness, hydrophobic thickness, headgroup-to-headgroup thickness, deuterium order parameter (S), and spin-lattice relaxation time (T)) obtained from simulations agree well with nearly all available experimental data. Read More

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http://dx.doi.org/10.1016/j.bbamem.2016.09.016DOI Listing
December 2016
7 Reads

DMSO-induced perturbation of thermotropic properties of cholesterol-containing DPPC liposomes.

Biochim Biophys Acta 2016 12 21;1858(12):3024-3031. Epub 2016 Sep 21.

Dipartimento di Chimica, Biologia e Biotecnologie, Università di Perugia, Via Elce di Sotto 8, 06123 Perugia, Italy. Electronic address:

Dimethyl sulfoxide (DMSO) is a universal solvent widely used in many fields, from basic research to industrial applications. At low concentration, it is the most important cryoprotectant agent against cellular damage caused during a freeze-thaw cycle. Although the effects of this cosolvent on the physico-chemical properties of a lipid bilayer have been extensively studied with both in vitro and in vivo experiments, the molecular mechanism of cryopreservation is not completely understood. Read More

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http://dx.doi.org/10.1016/j.bbamem.2016.09.012DOI Listing
December 2016
5 Reads

Mixed DPPC/POPC Monolayers: All-atom Molecular Dynamics Simulations and Langmuir Monolayer Experiments.

Biochim Biophys Acta 2016 12 21;1858(12):3120-3130. Epub 2016 Sep 21.

J. Heyrovský Institute of Physical Chemistry, Academy of Sciences of the Czech Republic, v.v.i., Dolejškova 3, 18223 Prague 8, Czech Republic; Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Flemingovo nám. 2, 16610 Prague 6, Czech Republic. Electronic address:

To elucidate the consequences of the saturated-unsaturated nature of lipid surface films, monolayers formed by an equimolar mixture of 1-palmitoyl-2-oleyl-sn-glycero-3-phosphocholine (POPC) and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) lipids are investigated in a wide range of surface pressures. As such mixtures share some features with naturally-occurring surfactants, for example the lung surfactant, the systems are studied at the temperature relevant for human body. All-atom molecular dynamics simulations and Langmuir trough experiments are employed. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00052736163031
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http://dx.doi.org/10.1016/j.bbamem.2016.09.015DOI Listing
December 2016
9 Reads

Stability of an amphipathic helix-hairpin surfactant peptide in liposomes.

Biochim Biophys Acta 2016 12 21;1858(12):3113-3119. Epub 2016 Sep 21.

Los Angeles Biomedical Research Institute at Harbor-UCLA Medical Center, Torrance, CA, United States; Department of Pediatrics, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA, United States.

Surfactant protein B (SP-B; 79 residues) is a member of the saposin superfamily and plays a pivotal role in lung function. The N- and C-terminal regions of SP-B, cross-linked by two disulfides, were theoretically predicted to fold as charged amphipathic helices, suggesting participation in surfactant activities. Previous studies with oxidized Super Mini-B (SMB), a construct based on the N- and C-regions of SP-B (i. Read More

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http://dx.doi.org/10.1016/j.bbamem.2016.09.014DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5096961PMC
December 2016
3 Reads
1 Citation

Enhancement of liposome mediated gene transfer by adding cholesterol and cholesterol modulating drugs.

Biochim Biophys Acta 2016 12 21;1858(12):3017-3023. Epub 2016 Sep 21.

Department of Physiology, College of Medicine, Yeungnam University, Daegu 42415, Republic of Korea. Electronic address:

Cholesterol is an important cell membrane component and has been used as co-lipid for cationic liposome to enhance gene delivery. However, the role of cholesterol in transfection efficiency has not been fully understood. In this study, transfection efficiency of liposome was measured after cholesterol was added to the cell culture medium. Read More

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http://dx.doi.org/10.1016/j.bbamem.2016.09.013DOI Listing
December 2016
1 Read

Quinolinic acid neurotoxicity: Differential roles of astrocytes and microglia via FGF-2-mediated signaling in redox-linked cytoskeletal changes.

Biochim Biophys Acta 2016 12 20;1863(12):3001-3014. Epub 2016 Sep 20.

Laboratório do Citoesqueleto, Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil. Electronic address:

QUIN is a glutamate agonist playing a role in the misregulation of the cytoskeleton, which is associated with neurodegeneration in rats. In this study, we focused on microglial activation, FGF2/Erk signaling, gap junctions (GJs), inflammatory parameters and redox imbalance acting on cytoskeletal dynamics of the in QUIN-treated neural cells of rat striatum. FGF-2/Erk signaling was not altered in QUIN-treated primary astrocytes or neurons, however cytoskeleton was disrupted. Read More

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http://dx.doi.org/10.1016/j.bbamcr.2016.09.014DOI Listing
December 2016
9 Reads

Endogenous TRPV1 stimulation leads to the activation of the inositol phospholipid pathway necessary for sustained Ca oscillations.

Biochim Biophys Acta 2016 12 20;1863(12):2905-2915. Epub 2016 Sep 20.

Anatomy, Department of Medicine, University of Fribourg, Route Albert-Gockel 1, Fribourg, CH-1700, Switzerland.

Sensory neuron subpopulations as well as breast and prostate cancer cells express functional transient receptor potential vanilloid type 1 (TRPV1) ion channels; however little is known how TRPV1 activation leads to biological responses. Agonist-induced activation of TRPV1 resulted in specific spatiotemporal patterns of cytoplasmic Ca signals in breast and prostate cancer-derived cells. Capsaicin (CAPS; 50μM) evoked intracellular Ca oscillations and/or intercellular Ca waves in all cell lines. Read More

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http://dx.doi.org/10.1016/j.bbamcr.2016.09.013DOI Listing
December 2016
2 Reads

Activation of apoptosis signalling pathways by reactive oxygen species.

Biochim Biophys Acta 2016 12 17;1863(12):2977-2992. Epub 2016 Sep 17.

Département des Sciences Biologiques (TOXEN, BIOMED), Université du Québec à Montréal, Montréal, Québec, Canada. Electronic address:

Reactive oxygen species (ROS) are short-lived and highly reactive molecules. The generation of ROS in cells exists in equilibrium with a variety of antioxidant defences. At low to modest doses, ROS are considered to be essential for regulation of normal physiological functions involved in development such as cell cycle progression and proliferation, differentiation, migration and cell death. Read More

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http://dx.doi.org/10.1016/j.bbamcr.2016.09.012DOI Listing
December 2016
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HIV-1 matrix-31 membrane binding peptide interacts differently with membranes containing PS vs. PI(4,5)P.

Biochim Biophys Acta 2016 12 15;1858(12):3071-3081. Epub 2016 Sep 15.

Biological Physics Group, Physics Department, Carnegie Mellon University, Pittsburgh, PA 15213, United States. Electronic address:

Efficient assembly of HIV-1 at the plasma membrane (PM) of the T-cell specifically requires PI(4,5)P. It was previously shown that a highly basic region (HBR) of the matrix protein (MA) on the Gag precursor polyprotein Pr55 is required for membrane association. MA is N-terminally myristoylated, which enhances its affinity to membranes. Read More

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http://dx.doi.org/10.1016/j.bbamem.2016.09.010DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5097034PMC
December 2016
1 Read

Control of human VDAC-2 scaffold dynamics by interfacial tryptophans is position specific.

Biochim Biophys Acta 2016 12 15;1858(12):2993-3004. Epub 2016 Sep 15.

Molecular Biophysics Laboratory, Department of Biological Sciences, Indian Institute of Science Education and Research, Bhopal, India. Electronic address:

Membrane proteins employ specific distribution patterns of amino acids in their tertiary structure for adaptation to their unique bilayer environment. The solvent-bilayer interface, in particular, displays the characteristic 'aromatic belt' that defines the transmembrane region of the protein, and satisfies the amphipathic interfacial environment. Tryptophan-the key residue of this aromatic belt-is known to influence the folding efficiency and stability of a large number of well-studied α-helical and β-barrel membrane proteins. Read More

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http://dx.doi.org/10.1016/j.bbamem.2016.09.011DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5091009PMC
December 2016
3 Reads

The Anticancer Agent, Di-2-Pyridylketone 4,4-Dimethyl-3-Thiosemicarbazone (Dp44mT), Up-Regulates the AMPK-Dependent Energy Homeostasis Pathway in Cancer Cells.

Biochim Biophys Acta 2016 12 15;1863(12):2916-2933. Epub 2016 Sep 15.

Molecular Pharmacology and Pathology Program, Department of Pathology and Bosch Institute, University of Sydney, Sydney, New South Wales, 2006, Australia. Electronic address:

Adenosine monophosphate-activated protein kinase (AMPK) is a cellular energy sensor that monitors ATP levels. There is also evidence that AMPK has onco-suppressive properties. Iron plays a crucial role in cellular energy transducing pathways and tumor cell proliferation. Read More

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http://dx.doi.org/10.1016/j.bbamcr.2016.09.011DOI Listing
December 2016
11 Reads

Interaction of the antimicrobial peptides caerin 1.1 and aurein 1.2 with intact bacteria by H solid-state NMR.

Biochim Biophys Acta 2016 12 14;1858(12):2959-2964. Epub 2016 Sep 14.

Department of Chemistry, Université du Québec à Montréal, P.O. Box 8888, Downtown Station, Montréal H3C 3P8, Canada. Electronic address:

Nuclear magnetic resonance (NMR) is commonly used to probe the effect of antimicrobial agents on bacterial membranes using model membrane systems. Ideally, considering the complexity of membranes, the interaction of molecules with membranes should be studied in vivo. The interactions of two antimicrobial peptides (AMPs) with intact Escherichia coli and Bacillus subtilis were investigated using deuterium solid-state NMR. Read More

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http://dx.doi.org/10.1016/j.bbamem.2016.09.009DOI Listing
December 2016
8 Reads

MicroRNA-455-3p modulates cartilage development and degeneration through modification of histone H3 acetylation.

Biochim Biophys Acta 2016 12 13;1863(12):2881-2891. Epub 2016 Sep 13.

Department of Joint Surgery, First Affiliated Hospital of Sun Yat-sen University, Guangzhou, Guangdong 510080, China. Electronic address:

Histone acetylation regulated by class I histone deacetylases (HDACs) plays a pivotal role in matrix-specific gene transcription and cartilage development. While we previously demonstrated that microRNA (miR)-455-3p is upregulated during chondrogenesis and can enhance early chondrogenesis, the mechanism underlying this process remains largely unclear. In this study, we characterized the effect of miR-455-3p on histone H3 acetylation and its role during cartilage development and degeneration. Read More

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http://dx.doi.org/10.1016/j.bbamcr.2016.09.010DOI Listing
December 2016
12 Reads

Correlated trapping of sugar molecules by the trimeric protein channel  chitoporin.

Biochim Biophys Acta 2016 12 14;1858(12):3032-3040. Epub 2016 Sep 14.

School of Physics, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand. Electronic address:

The protein channel chitoporin (ChiP), which is used by marine bacteria to translocate selected sugar molecules through the outer cell membrane, is studied via single channel current measurements in water and heavy water sugar solutions. The dynamic trapping and escape probabilities of sugar molecules from different monomers in the trimeric channel are characterized, including their dependence on channel orientation and sensitivity to a deuterium isotope effect. A detailed analysis of stochastic current fluctuations reveals that the trapping properties of chitoporin exhibit memory effects: the rate of trapping transitions depends on the previous sequence of transitions; and intermonomer correlations: the average trapping rate of an unblocked monomer is larger when its neighboring monomers are blocked. Read More

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http://dx.doi.org/10.1016/j.bbamem.2016.09.007DOI Listing
December 2016
96 Reads

Comparison between mouse and sea urchin orthologs of voltage-gated proton channel suggests role of S3 segment in activation gating.

Biochim Biophys Acta 2016 12 13;1858(12):2972-2983. Epub 2016 Sep 13.

Lab. of Integrative Physiology, Department of Physiology, Graduate School of Medicine, Osaka University, Suita, Osaka 565-0871, Japan; Section of Developmental Neurophysiology, Okazaki Institute for Integrative Bioscience (National Institute for Physiological Sciences), Okazaki, Aichi, Japan; National Institute of Natural Sciences, Okazaki, Aichi, Japan; Graduate School of Frontier Bioscience, Osaka University, Suita, Osaka 565-0871, Japan. Electronic address:

The voltage-gated proton channel, Hv1, is expressed in blood cells, airway epithelium, sperm and microglia, playing important roles in diverse biological contexts including phagocytosis or sperm maturation through its regulation of membrane potential and pH. The gene encoding Hv1, HVCN1, is widely found across many species and is also conserved in unicellular organisms such as algae or dinoflagellates where Hv1 plays role in calcification or bioluminescence. Voltage-gated proton channels exhibit a large variation of activation rate among different species. Read More

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http://dx.doi.org/10.1016/j.bbamem.2016.09.008DOI Listing
December 2016
3 Reads

Integrin-linked kinase and ELMO2 modulate recycling endosomes in keratinocytes.

Biochim Biophys Acta 2016 12 11;1863(12):2892-2904. Epub 2016 Sep 11.

Dept. of Physiology and Pharmacology, Children's Health Research Institute and Lawson Health Research Institute, University of Western Ontario, London, Ontario N6A 5C1, Canada. Electronic address:

The formation of tight cell-cell junctions is essential in the epidermis for its barrier properties. In this tissue, keratinocytes follow a differentiation program tightly associated with their movement from the innermost basal to the outer suprabasal layers, and with changes in their cell-cell adhesion profile. Intercellular adhesion in keratinocytes is mediated through cell-cell contacts, including E-cadherin-based adherens junctions. Read More

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http://dx.doi.org/10.1016/j.bbamcr.2016.09.007DOI Listing
December 2016
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Erythroid cell mitochondria receive endosomal iron by a "kiss-and-run" mechanism.

Biochim Biophys Acta 2016 12 11;1863(12):2859-2867. Epub 2016 Sep 11.

Lady Davis Institute for Medical Research, Jewish General Hospital, Montreal, Quebec, Canada; Department of Physiology, McGill University, Montreal, Quebec, Canada. Electronic address:

In erythroid cells, more than 90% of transferrin-derived iron enters mitochondria where ferrochelatase inserts Fe into protoporphyrin IX. However, the path of iron from endosomes to mitochondrial ferrochelatase remains elusive. The prevailing opinion is that, after its export from endosomes, the redox-active metal spreads into the cytosol and mysteriously finds its way into mitochondria through passive diffusion. Read More

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http://dx.doi.org/10.1016/j.bbamcr.2016.09.008DOI Listing
December 2016
8 Reads

Privileged crosstalk between TRPV1 channels and mitochondrial calcium shuttling machinery controls nociception.

Biochim Biophys Acta 2016 12 11;1863(12):2868-2880. Epub 2016 Sep 11.

Department of Medical Neurobiology, Institute for Medical Research Israel-Canada, Faculty of Medicine, The Hebrew University, Jerusalem 91120, Israel; The Edmond and Lily Safra Center for Brain Sciences, The Hebrew University, Jerusalem 91120, Israel. Electronic address:

The nociceptive noxious heat-activated receptor - TRPV1, conducts calcium and sodium, thus producing a depolarizing receptor potential, leading to activation of nociceptive neurons. TRPV1-mediated calcium and sodium influx is negatively modulated by calcium, via calcium-dependent desensitization of TRPV1 channels. A mitochondrial Ca uniporter - MCU, controls mitochondrial Ca entry while a sodium/calcium transporter - NCLX shapes calcium and sodium transients by mediating sodium entry into and removing calcium from the mitochondria. Read More

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http://dx.doi.org/10.1016/j.bbamcr.2016.09.009DOI Listing
December 2016
11 Reads

Bacillus subtilis alters the proportion of major membrane phospholipids in response to surfactin exposure.

Biochim Biophys Acta 2016 12 9;1858(12):2965-2971. Epub 2016 Sep 9.

Department of Genetics and Microbiology, Faculty of Science, Charles University in Prague, Viničná 5, 128 44, Prague 2, Czech Republic. Electronic address:

Surfactin, an anionic lipopeptide produced by Bacillus subtilis, is an antimicrobial that targets the cytoplasmic membrane. Nowadays it appears increasingly apparent that the mechanism of resistance against these types of antibiotics consists of target site modification. This prompted us to investigate whether the surfactin non-producing strain B. Read More

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http://dx.doi.org/10.1016/j.bbamem.2016.09.006DOI Listing
December 2016
4 Reads

Isolation of yeast complex IV in native lipid nanodiscs.

Biochim Biophys Acta 2016 12 13;1858(12):2984-2992. Epub 2016 Sep 13.

Department of Biochemistry and Biophysics, The Arrhenius Laboratories for Natural Sciences, Stockholm University, SE-106 91 Stockholm, Sweden. Electronic address:

We used the amphipathic styrene maleic acid (SMA) co-polymer to extract cytochrome c oxidase (CytcO) in its native lipid environment from S. cerevisiae mitochondria. Native nanodiscs containing one CytcO per disc were purified using affinity chromatography. Read More

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http://dx.doi.org/10.1016/j.bbamem.2016.09.004DOI Listing
December 2016
10 Reads

The effect of polyethylene glycol-modified lipids on the interaction of HIV-1 derived peptide-dendrimer complexes with lipid membranes.

Biochim Biophys Acta 2016 12 9;1858(12):3005-3016. Epub 2016 Sep 9.

Faculty of Mathematics, Physics and Informatics, Comenius University, Mlynska dolina, 842 48 Bratislava, Slovakia. Electronic address:

In this study, dendrimers have been purposed as an alternative approach for delivery of HIV peptides to dendritic cells. We have investigated the interaction of dendriplexes formed from polyanionic HIV peptide Nef and cationic carbosilane dendrimer (CBD) with model lipid membranes - large unilamellar vesicles (LUVs), Langmuir monolayers and supported lipid membranes (sBLMs) containing various molar ratio of zwitterionic 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy (polyethylene glycol)-2000] (DSPE-PEG). In our experiments, the lipid membranes represented the model of the plasma membrane of the cell. Read More

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http://dx.doi.org/10.1016/j.bbamem.2016.09.005DOI Listing
December 2016
7 Reads

Proofreading of substrate structure by the Twin-Arginine Translocase is highly dependent on substrate conformational flexibility but surprisingly tolerant of surface charge and hydrophobicity changes.

Biochim Biophys Acta 2016 12 13;1863(12):3116-3124. Epub 2016 Sep 13.

School of Biosciences, University of Kent, Canterbury CT2 7NJ, United Kingdom. Electronic address:

The Tat system transports folded proteins across the bacterial plasma membrane, and in Escherichia coli preferentially transports correctly-folded proteins. Little is known of the mechanism by which Tat proofreads a substrate's conformational state, and in this study we have addressed this question using a heterologous single-chain variable fragment (scFv) with a defined structure. We introduced mutations to surface residues while leaving the folded structure intact, and also tested the importance of conformational flexibility. Read More

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http://dx.doi.org/10.1016/j.bbamcr.2016.09.006DOI Listing
December 2016
13 Reads

Exploring the raft-hypothesis by probing planar bilayer patches of free-standing giant vesicles at nanoscale resolution, with and without Na,K-ATPase.

Biochim Biophys Acta 2016 12 9;1858(12):3041-3049. Epub 2016 Sep 9.

MEMPHYS, University of Southern Denmark, Odense 5230, Denmark.

The structure of functional lipid domains (rafts) in biological membranes has for long time been unresolved due to their small length scales and transient nature. These cooperative properties of the lipid bilayer matrix are modelled by free-standing giant unilammellar vesicles (GUVs) with well-characterized lipid composition. We review a series of recent advances in preparation and analysis of GUVs, which allows for characterization of small domains by high-resolution imaging techniques. Read More

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http://dx.doi.org/10.1016/j.bbamem.2016.09.001DOI Listing
December 2016

Effects of mutations in Wnt/β-catenin, hedgehog, Notch and PI3K pathways on GSK-3 activity-Diverse effects on cell growth, metabolism and cancer.

Biochim Biophys Acta 2016 12 6;1863(12):2942-2976. Epub 2016 Sep 6.

Dipartimento di Scienze Biomediche e Neuromotorie, Università di Bologna, Bologna, Italy.

Glycogen synthase kinase-3 (GSK-3) is a serine/threonine kinase that participates in an array of critical cellular processes. GSK-3 was first characterized as an enzyme that phosphorylated and inactivated glycogen synthase. However, subsequent studies have revealed that this moon-lighting protein is involved in numerous signaling pathways that regulate not only metabolism but also have roles in: apoptosis, cell cycle progression, cell renewal, differentiation, embryogenesis, migration, regulation of gene transcription, stem cell biology and survival. Read More

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http://dx.doi.org/10.1016/j.bbamcr.2016.09.004DOI Listing
December 2016
35 Reads