2,592 results match your criteria Biochemistry and molecular biology international[Journal]


Resveratrol inhibits copper ion-induced and azo compound-initiated oxidative modification of human low density lipoprotein.

Biochem Mol Biol Int 1999 Jun;47(6):1089-96

Department of Cardiology, Nanjing Medical University, China.

To investigate whether resveratrol, a polyphenolic compound in red wine, affects the oxidation of human low density lipoprotein (LDL), LDL purified from normolipidemic subjects was subjected to Cu(2+)-induce and azo compound-initiated oxidative modification, with and without the addition of varying concentrations of resveratrol. Modification of LDL was assessed by the formation of thiobarbituric acid reactive substances (TBARS) and changes in the relative electrophoretic mobility (REM) of LDL on agarose gels. Resveratrol (50 microM) reduced TBARS and REM of LDL during Cu(2+)-induced oxidation by 70. Read More

View Article

Download full-text PDF

Source

Purification and characterization of tripeptidyl peptidase I from Dictyostelium discoideum.

Biochem Mol Biol Int 1999 Jun;47(6):1079-88

Department of Chemistry, University of San Francisco, CA 94117-1040, USA.

A tripeptidyl peptidase I from Dictyostelium discoideum was purified 744-fold to near homogeneity. The enzyme is 214 kDa in size and is composed of two monomers with a M(r) of 107 kDa. It has two pH optima at pH 4. Read More

View Article

Download full-text PDF

Source

Purification and partial characterization of a new proteolytic enzyme from the venom of Bothrops moojeni (CAISSACA).

Biochem Mol Biol Int 1999 Jun;47(6):1069-77

Departamento de Ciências, Faculdade de Filosofia, Ciências e Letras, FAFI, Araguari-MG, Brazil.

A basic serine protease which is active on casein and fibrinogen was purified from Bothrops moojeni venom using a single step chromatography on a CM-Sepharose fast flow column. The enzyme, MOO3, was not hemorrhagic and presented only a trace of blood-clotting activity. Synthetic chromogenic substrates (azoacasein and azoalbumin) where not hydrolyzed by MOO3. Read More

View Article

Download full-text PDF

Source

Denitration of peroxynitrite-treated proteins by "protein nitratases" from dog prostate.

Biochem Mol Biol Int 1999 Jun;47(6):1061-7

Division of Science and Mathematics, Bethune-Cookman College, Daytona Beach, Florida 32114, USA.

Putative "protein nitratases," which catalyze denitration of peroxynitrite (PN)-treated, proteins, were detected in the crude extract of dog prostate. Nitratase activity was monitored by the decreased intensity of nitrotyrosine immunoreactive-bands in Western blot and increased nitrate level in dialysate of incubation mixture, which contained prostate crude extract, protease inhibitors and a PN-treated substrate, such as treated histone (III-S), BSA, invertase, or polylysine. Nitratases were activated by preincubation with m-calpain/Ca2+. Read More

View Article

Download full-text PDF

Source

Changes of nuclear membrane fluidity during rat liver regeneration.

Biochem Mol Biol Int 1999 Jun;47(6):1049-59

Institute of General Pathology, School of Medicine, University of Perugia, Italy.

We have previously shown that the nuclear membrane fluidity is affected by lipid composition changes and that is very high, particularly in the hydrophobic core. The aim of this work is to study the modifications of nuclear membrane fluidity in relation to the cell cycle. Since compensatory hepatic growth is an informative and well characterised model for natural cell proliferation, the nuclear membrane fluidity, detected by two fluorescent probes, was studied at various regenerating times, ranging from 0 to 30 hours after partial hepatectomy. Read More

View Article

Download full-text PDF

Source

Preparation and characterization of beta 1-bungarotoxin bispecific monoclonal antibody.

Authors:
C C Yang H L Chan

Biochem Mol Biol Int 1999 Jun;47(6):1039-48

Department of Life Science, National Tsing Hua University, Hsinchu, Taiwan, Republic of China.

A hybrid hybridoma (tetradoma) that produces bispecific monoclonal antibodies (mAbs)2, which recognize two different epitopes on the A chain of beta 1-bungarotoxin (beta 1-bgt) at peptide sequences 46-51 and 100-106, has been obtained by fusing two hybridoma cell lines. The bispecific mAb were observed to inhibit 98% of the enzymatic activity of beta 1-bgt and neutralize its lethal toxicity completely. The avidity between the bispecific mAb and beta 1-bgt was noted to be 4. Read More

View Article

Download full-text PDF

Source

Noncompetitive, Ca(2+)-independent inhibition of pyruvate dehydrogenase phosphatase by fluphenazine.

Biochem Mol Biol Int 1999 Jun;47(6):1029-37

Laboratory of Membrane Biochemistry and Biophysics, National Institute on Alcohol Abuse and Alcoholism, Rockville, MD 20852, USA.

The effects of two different classes of calmodulin antagonists on the catalytic activities of purified pyruvate dehydrogenase (PDH) phosphatase and PDH complex (PDC) were studied. In general, PDH phosphatase was more strongly inhibited than PDC by the calmodulin antagonists with the following potency order: fluphenazine > chlorpromazine > thioridazine > triflupromazine. Promazine and two sulfonamides (W-5 and W-7) did not suppress PDH phosphatase activity at 1 mM concentrations, while about 20% of PDC activity was inhibited by these antagonists. Read More

View Article

Download full-text PDF

Source

The antioxidant status of patients subjected to total body irradiation.

Biochem Mol Biol Int 1999 Jun;47(6):1019-27

Department of Human Nutrition and Metabolism, Hebrew University-Hadassah School of Medicine, Jerusalem, Israel.

Background And Purpose: Total body irradiation (TBI) is a routine preconditioning procedure for the treatment of leukemia and aplastic anemia, prior to bone marrow transplantation (BMT). Ionizing radiation generates reactive oxygen derived species (ROS) that can be removed by antioxidants. Our purpose is to determine the antioxidant status of patients undergoing TBI by evaluating the oxidant stress and their antioxidant capacity. Read More

View Article

Download full-text PDF

Source

Overexpression of human testis antigens in Escherichia coli host cells is influenced by site of expression and the induction temperature.

Biochem Mol Biol Int 1999 Jun;47(6):1009-18

Department of Biological Sciences, University of Newcastle, Callaghan, Australia.

A panel of twenty human testis cDNA clones were expressed in an Escherichia coli expression system and six clones were found to express identifiable fusion polypeptides. Expression was found to be influenced not only by the site of localization of the polypeptide in the host cells, but also by the temperature used for induction. This emphasized the need for cytoplasmic and periplasmic expression of new antigens of unknown properties, as well as the use of temperatures of 30 degrees C or lower. Read More

View Article

Download full-text PDF

Source

The amino acid sequence of ovocleidin 17, a major protein of the avian eggshell calcified layer.

Authors:
K Mann F Siedler

Biochem Mol Biol Int 1999 Jun;47(6):997-1007

Max-Planck-Institut für Biochemie, Martinsried, Germany.

The amino acid sequence of ovocleidin 17, a major protein of the chicken eggshell calcified layer, contains 142 amino acids including 2 phosphorylated serines. Data base searches show that ovocleidin belongs to a heterogeneous group of proteins consisting of a single C-type lectin domain (CTL). The most similar sequences with an average of 30% identical amino acids were those of pancreatic stone protein (lithostathine) and lectins and anticoagulant proteins from snake venom. Read More

View Article

Download full-text PDF

Source
June 1999
1 Read

Stabilization of the T-state of human hemoglobin by proflavine, an antiseptic drug.

Biochem Mol Biol Int 1999 Jun;47(6):991-5

Department of Biology, University of Rome Tre, Italy.

The effect of proflavine (3,6-diaminoacridine), an antiseptic drug, on the spectroscopic and oxygen binding properties of ferrous human adult hemoglobin (Hb) has been investigated. Upon binding of proflavine to the nitric oxide derivative of ferrous human adult hemoglobin (HbNO), the X-band EPR spectrum displays the characteristics which have been attributed to the T-state of the ligated tetramer. In parallel, oxygen affinity for the deoxygenated derivative of ferrous human adult Hb decreases in the presence of proflavine. Read More

View Article

Download full-text PDF

Source

Superoxide anion and hydroxyl radical scavenging activities of vegetable extracts measured using electron spin resonance.

Biochem Mol Biol Int 1999 Jun;47(6):979-89

Faculty of Health and Welfare Science, Department of Nutritional Science, Okayama Prefectural University, Soja, Japan.

Radical scavenging by reconstituted lyophilized powders of water extracts from 16 common vegetables was measured using electron spin resonance (ESR) with 5,5-dimethyl-1-pyrroline-N-oxide (DMPO), hydroxyl radicals, (.OH) or superoxide anion radicals (O2.-), as DMPO-OH or DMPO-OOH spin adducts. Read More

View Article

Download full-text PDF

Source

Expression of the divergent transcription unit containing the yeast PET122 and OXA1 genes.

Biochem Mol Biol Int 1999 Jun;47(6):971-7

GRECC (Geriatrics Research and Education Clinical Center), John L. McClellan Memorial VA Hospital, Little Rock, AR 72205, USA.

The nuclear PET122 gene of S. cerevisiae encodes a mitochondrial-localized protein that activates initiation of translation of the mitochondrial mRNA from the COX3 gene, which encodes subunit III of cytochrome c oxidase. The PET122 locus contains two divergent transcription units: one is involved in expression of PET122 mRNA and the mRNA for an adjacent gene OXA1, which is also required for cytochrome c oxidase biogenesis, and the other is involved in expression of an antisense RNA that is complementary to about two thirds of the PET122 mRNA and an adjacent gene YER152C of unknown function. Read More

View Article

Download full-text PDF

Source

Evidence of carbamoylphosphate induced conformational changes upon binding to human ornithine carbamoyltransferase.

Biochem Mol Biol Int 1999 Jun;47(6):965-70

Dipartimento di Chimica Organica e Biologica, Università di Messina, Italy.

Human liver ornithine carbamoyltransferase undergoes absorbance changes in the UV region upon formation of the carbamoylphosphate-norvaline-enzyme ternary complex. The UV changes are similar in the presence of carbamoylphosphate alone, whilst they are lower in the presence of ornithine or norvaline alone. The extent of the UV changes correlates with the enzyme susceptibility to proteolytic degradation. Read More

View Article

Download full-text PDF

Source

Synthetic insulin fragment with insulin-like biological activity.

Biochem Mol Biol Int 1999 Jun;47(6):957-63

Institute of Biomedical Chemistry, Russian Academy of Medical Science, Moscow, Russia.

An insulin fragment, representing the C-terminal functionally important site of its molecule and responsible for receptor binding, was synthesized. The fragment consists of two peptides: a dipeptide (A 20-21) and an octapeptide (B 19-26), linked with a disulfide bond (A20-B19). The biological activity of the newly synthesized fragment relative to insulin was assayed for the influence on glycogenesis and for the ability to stimulate glucose uptake. Read More

View Article

Download full-text PDF

Source

Porphyria-induced hepatic porphyrinogen carboxy-lyase inhibitor and its interaction with the active site(s) of the enzyme.

Biochem Mol Biol Int 1999 Jun;47(6):945-56

Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Argentina.

Porphyrinogen carboxy-lyase is an enzyme that sequentially decarboxylates uroporphyrinogen III (8-COOH) to yield coproporphyrinogen III (4-COOH). In mammals this enzyme activity is impaired by hexachlorobenzene treatment, through generation of an enzyme inhibitor. The interaction of porphyrinogen carboxy-lyase inhibitor, extracted from the liver of hexachlorobenzene-treated rats, with substrate decarboxylation sites on the enzyme, was studied using four different carboxylated substrates belonging to the isomeric III series of naturally-formed porphyrinogens containing 8-,7-,6- and 5-COOH. Read More

View Article

Download full-text PDF

Source
June 1999
1 Read

RAPD profile based genetic characterization of chemotypic variants of Artemisia annua L.

Biochem Mol Biol Int 1999 Jun;47(6):935-44

Central Institute of Medicinal and Aromatic Plants, Lucknow, India.

The annual herbaceous plant, Artemisia annua L., belonging to family Asteraceae, is the natural source of the highly potent antimalarial compound, artemisinin, besides producing valuable essential oil. The plant is at present the sole commercial source for artemisinin production since all the chemical syntheses are non-viable. Read More

View Article

Download full-text PDF

Source

Activation of bradykinin B2 receptors increases calcium entry and intracellular mobilization in C9 liver cells.

Biochem Mol Biol Int 1999 Jun;47(6):927-33

Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, México D. F.

In C9 rat liver cells bradykinin and kallidin increased (approximately 2-fold) the intracellular concentration of calcium, but the B1 agonist, des-Arg9-bradykinin did not. The effect of bradykinin was inhibited by the B2 antagonists, Hoe 140 and N-alpha-adamantaneacetyl-D-Arg-[Hyp3, Thi5,8, D-Phe7]-bradykinin, but not by the B1 antagonist, des-Arg9-[Leu8]-bradykinin. The action of bradykinin was diminished, but not abolished, in medium without calcium. Read More

View Article

Download full-text PDF

Source

The lysine and methionine rich basic subunit of buckwheat grain legumin: some results of a structural study.

Biochem Mol Biol Int 1999 Jun;47(6):921-6

Department of Botany, North Eastern Hill University, Shillong, India.

The 26 kD basic subunit of 280 kD buckwheat grain legumin has been partially characterized by measurement of its fluorescence and CD spectra. The protein has 22% alpha-helix, 36% beta-sheet, 12% beta-turn and 30% random coil secondary structure. In comparison with the basic subunits of other legumin-type proteins, the buckwheat legumin subunit has a high content of lysine and methionine. Read More

View Article

Download full-text PDF

Source

Total reactive antioxidant potential in human saliva of smokers and non-smokers.

Biochem Mol Biol Int 1999 Jun;47(6):911-20

Institute of Oncology, Russian Academy of Medical Sciences, Tomsk, Russia.

Uric acid is the most important non-enzymatic antioxidant present in human saliva. There is a great variability among individuals, both in salivary uric acid content and saliva total reactive antioxidant potential (TRAP). The uric acid present in saliva correlates with plasma uric acid, suggesting that the former is imported from plasma. Read More

View Article

Download full-text PDF

Source

Categorizing reactivity of bacteriorhodopsin cysteine mutants crosslinking to 4-bromoretinal.

Biochem Mol Biol Int 1999 May;47(5):773-80

Department of Physical Sciences, South Carolina State University, Orangeburg 29117, USA.

The structure of bacteriorhodopsin (bR) has been probed by a large number of experimental methods. In earlier work distance constraints measured from the 1BRD Brookhaven structure (1, 2) were used to guide site-directed mutagenesis/affinity labeling experiments (3-5). In the present study we report on the use of limited molecular dynamics (MD) investigations of the same bR/affinity label system. Read More

View Article

Download full-text PDF

Source

Purification and characterization of cytochrome C from camel muscle.

Biochem Mol Biol Int 1999 May;47(5):899-907

Department of Biochemistry, College of Science, King Saud University, Riyadh, Saudi Arabia.

Cytochrome C was purified from camel skeletal muscles using ammonium sulphate fractionation and gel filtration on Sephadex G25 and Sephecryl S200 columns. The preparations were pure by SDS-PAGE criteria, with molecular weight of 12,300 Dalton. The electrophoretic mobility of camel cytochrome C was the same as that of the horse heart. Read More

View Article

Download full-text PDF

Source

Cloning and expression of human cDNA encoding human homologue of pituitary tumor transforming gene.

Biochem Mol Biol Int 1999 May;47(5):891-7

Molecular and Cellular Biology Research Group, Korea Research Institute of Bioscience and Biotechnology, Taejon, Korea.

Recently, a potent transforming gene which was exclusively expressed in rat pituitary tumor but not in normal pituitary had been isolated and named as pituitary tumor transforming gene (PTTG). A cDNA clone encoding human homologue of rat PTTG was isolated from human fetal liver cDNA library. It contained an open reading frame of 603 base pairs predicting a protein composed of 201 amino acids with a calculated molecular weight of 26 kDa. Read More

View Article

Download full-text PDF

Source

Human anti-DNA autoantibodies and induced antibodies against ROS-modified-DNA show similar antigenic binding characteristics.

Authors:
K Alam R Ali

Biochem Mol Biol Int 1999 May;47(5):881-90

Department of Biochemistry, Faculty of Medicine, A. M. U. Aligarh, India.

Alterations in DNA structure by hydroxyl radical modification was characterized by UV spectroscopy, Tm, nuclease S1 digestibility and base modification. In view of indicted role of oxygen free radicals in human diseases, an attempt has been made to precisely compare the antigen binding properties of induced antibodies against hydroxyl radical modified DNA with those of naturally occurring anti-DNA autoantibodies. Antibodies induced against ROS-DNA showed diverse antigen binding characteristics which were comparable with those derived from SLE patients. Read More

View Article

Download full-text PDF

Source
May 1999
3 Reads

The involvement of hexokinases in trehalose synthesis.

Biochem Mol Biol Int 1999 May;47(5):873-80

Departamento de Bioquímica, Universidade Federal do Rio de Janeiro, Brasil.

Yeast cells harboring a MAL2-8c gene accumulate trehalose during the transition phase of growth on glucose due to the presence of the ADPG-dependent trehalose 6-phosphate synthase. Under these conditions, glucokinase appeared not to provide G-6-P for trehalose synthesis and the two hexokinases seemed to act synergistically. After incubation in d-xylose, trehalose levels in these cells dropped almost in 90%, confirming the involvement of both hexokinases in the accumulation of this carbohydrate. Read More

View Article

Download full-text PDF

Source

Effect of dose-rate and dose fractionation on radiation-induced hemolysis of human erythrocytes.

Biochem Mol Biol Int 1999 May;47(5):865-72

Department of Molecular Biophysics, University of Lódz, Poland.

Human erythrocytes suspended in an isotonic Na-phosphate buffer, pH 7.4 (hematocrit 2%) were exposed under air to gamma radiation at a dose rates of 2.2 kGy. Read More

View Article

Download full-text PDF

Source
May 1999
1 Read

Regulation of an 8-kDa peptide involved in testosterone production by luteinizing hormone in rat Leydig cells.

Authors:
P Ramaraj A J Rao

Biochem Mol Biol Int 1999 May;47(5):857-63

Department of Diabetes, Endocrinology & Metabolism, University of Hope National Medical Centre, Duarte, CA 91007, USA.

Results of Western blot analysis carried out with an interstitial cell extract from male guinea pig and ovarian extract from immature female rats administered equine chorionic gonadotropin (eCG) provide supportive evidence to our earlier suggestion that an 8-kDa peptide is involved in acquisition of steroidogenic capacity by the rat Leydig cells. It was found that though the signal was observed in other tissues such as liver, kidney and lung which do not produce gonadal hormones, the peptide was modulated only by lutenizing hormone (LH) in the rat Leydig cells. Read More

View Article

Download full-text PDF

Source

Changes in membrane-bound leucine aminopeptidase activity during maturation and ageing of brain.

Biochem Mol Biol Int 1999 May;47(5):851-6

Department of Physiology, Medical School, University of the Basque Country, Leioa, Vizcaya, Spain.

Aminopeptidases are believed to be enzymes that regulate the activity of various neuropeptides. However, their physiological role, as well as their mechanisms of regulation, are not well understood. To analyze a part of the regulatory mechanisms that control the activity of these enzymes, the subcellular distribution of membrane-bound leucyl aminopeptidase activity was studied in rat brain during development and ageing. Read More

View Article

Download full-text PDF

Source

Can serum amyloid A or macrophage colony stimulating factor serve as marker of amyloid formation process?

Biochem Mol Biol Int 1999 May;47(5):845-50

1st Medical Department, 1st Medical Faculty, Charles University, Prague, Czech Republic.

Amyloid formation depends on amyloid precursor production and is influenced by the activity of the underlying disorder and mediated by some proinflammatory cytokines. In this pilot study we tried to find some specific markers that could establish the activity of the disease. We investigated 45 samples of sera and 38 samples of urine from patients (pts) with secondary amyloidosis (AA), primary amyloidosis (AL), systemic autoimmune diseases with renal impairment (Vasc) and healthy controls (Co). Read More

View Article

Download full-text PDF

Source

Fatty acid profile of Escherichia coli during the heat-shock response.

Biochem Mol Biol Int 1999 May;47(5):835-44

Dept. Biochemistry, Centro de Investigación y de Estudios Avanzados del I. P. N., A. P., México, D. F.

The possible changes in the fatty acid profile of Escharichia coli during heat-shock have been investigated. Bacteria growing in steady-state at 30 degrees C were subjected to an abrupt temperature upshift to 45 degrees C and held at the high temperature for various periods of time in order to elicit the heat-shock response. Fatty acid compositions of lipids extracted from samples taken at different times after the temperature upshift, as well as from cultures in steady-state at 30 and 45 degrees C, were determined by gas-chromatography. Read More

View Article

Download full-text PDF

Source
May 1999
1 Read

Chemical modification studies on the glucose/mannose specific lectins from field and lablab beans.

Authors:
S K Nadimpalli

Biochem Mol Biol Int 1999 May;47(5):825-34

Department of Biochemistry, University of Hyderabad, India.

Seeds of Dolichos lablab var. lignosus (field beans) and variety typicus (lablab beans) contain glucose/mannose specific lectins that have been affinity purified and well characterised (Siva Kumar N., and Rajagopal Rao, D. Read More

View Article

Download full-text PDF

Source

Effect of alpha lipoic acid amide on hexachlorobenzene porphyria.

Biochem Mol Biol Int 1999 May;47(5):815-23

Dpto. de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina.

The aim of this work is to study the effect of thioctamide--the commercial form of alpha lipoic acid amide--on the porphyrinogenic action of hexachlorobenzene (HCB). For this purpose, porphyria was induced in rats by chronic HCB treatment, with or without simultaneous thioctamide administration. Two different groups of rats were used as reference: one treated with vehicle (control) and the other treated with thioctamide (TO). Read More

View Article

Download full-text PDF

Source

Cloning, sequencing, expression and characterization of the manganese superoxide dismutase gene from Vibrio alginolyticus.

Biochem Mol Biol Int 1999 May;47(5):803-14

Institute of Marine Biotechnology, National Taiwan Ocean University, Keelung, Taiwan, R. O. C.

The sodA gene coding for manganese superoxide dismutase from the marine microorganism Vibrio alginolyticus was cloned, sequenced and over-expressed in Escherichia coli using the pET20b (+) expression vector. The full-length gene was consisted of 603bp open reading frame, which encoded a polypeptide of 201 amino acid residues, with a calculated molecular weight of 22672Da. The deduced amino acid sequence of the sodA showed considerable homology to other Mn-SODs. Read More

View Article

Download full-text PDF

Source

Inhibition of eukaryotic dna polymerase alpha by persimmon (Diospyros kaki) extract and related polyphenols.

Biochem Mol Biol Int 1999 May;47(5):795-801

Department of Agricultural Chemistry, Faculty of Bioresources, Mie University, Japan.

The effects of persimmon extract (Diospyros kaki) and related polyphenols on eukaryotic DNA polymerase alpha were examined. It was found that persimmon extract, epigallocatechin gallate, and epicatechin gallate strongly inhibited the activity of DNA polymerase alpha purified from calf thymus. Among these polyphenols, persimmon extract had the most potent effect on DNA polymerase alpha activity and the concentration of persimmon extract producing 50% inhibition of the activity was 0. Read More

View Article

Download full-text PDF

Source

Induction of apoptosis by selenite and selenodiglutathione in HL-60 cells: correlation with cytotoxicity.

Biochem Mol Biol Int 1999 May;47(5):781-93

Department of Biological Sciences, Korea Advanced Institute of Science and Technology, South Korea.

Effects of selenite and selenodiglutathione, an initial metabolite of selenite, on the induction of apoptosis and cytotoxicity were investigated in human promyelocytic leukemia HL-60 cells. Treatment of selenite or selenodiglutathione resulted in concentration-dependent cytotoxicity, measured by lactate dehydrogenase leakage assay, and by tetrazolium salt reduction assay. Selenodiglutathione has been shown to exert more cytotoxic effect than selenite in both assay systems. Read More

View Article

Download full-text PDF

Source
May 1999
1 Read

Mitochondrial changes during the apoptotic process of HeLa cells exposed to cisplatin.

Biochem Mol Biol Int 1999 May;47(5):765-71

Laboratorio de Biología Molecular, Instituto Nacional de Cancerología, México, D. F.

HeLa cells undergo apoptosis after exposure to cisplatin. Since mitochondria have recently been proposed as a probable effector of this type of cell death, we performed an analysis using the fluorescent cation rhodamine 123, which is transported actively by this organelle. Cisplatin induces a decrease in the mitochondrial staining, as assessed by cytofluorometric analysis. Read More

View Article

Download full-text PDF

Source

Nucleoid proteins of pea chloroplasts: detection of a protein homologous to ribosomal protein.

Biochem Mol Biol Int 1999 May;47(5):757-63

Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow, Russia.

Basic proteins were isolated from purified pea chloroplast nucleoids by acid extraction. Using RP-HPLC, the component composition of the basic proteins was studied. SDS-PAGE of major HPLC-fractions showed that the basic nucleoid proteins are heterogeneous with mol. Read More

View Article

Download full-text PDF

Source

Kinetic properties of ATP-dependent phosphofructokinase from grapefruit juice sacs: effect of TCA cycle intermediates.

Biochem Mol Biol Int 1999 May;47(5):749-56

Kennedy-Leigh Centre for Horticultural Research, Hebrew University of Jerusalem, Rehovot, Israel.

Grapefruit juice sac ATP-PFK was studied kinetically for its substrates ATP and Fru-6-P at pH = 7.5. The Km for ATP is equal to 39. Read More

View Article

Download full-text PDF

Source

Influence of atropine on carbachol dual effect on Ca2+ mobilization in SH-SY5Y neuroblastoma cells.

Biochem Mol Biol Int 1999 May;47(5):743-7

Institute of Chemical Physics, University of Tartu, Estonia.

The muscarinic receptor stimulated mobilisation of calcium ions in SH-SY5Y neuroblastoma cells was measured as a function on carbachol and atropine concentrations. The combined application of this pair of muscarinic agonist and antagonist yielded a set of bell-shaped dose-response curves. In the presence of atropine the cell responses were smaller and the up-going phase of these relationships was shifted towards higher agonist concentration, while the down-going phase of these curves was not influenced by the antagonist. Read More

View Article

Download full-text PDF

Source

Phenotypic selection: a successful strategy to fix major genes of hypertension.

Biochem Mol Biol Int 1999 May;47(5):735-42

Department of Laboratory Animal Science, University of Greifswald, Karlsburg, Germany.

Hypertension is dominantly inherited in cross hybrids between hypertensive SHR/Mol and normotensive BB/OK rats. We used these cross hybrids for repeated backcrossing of selected hypertensive animals onto normotensive BB/OK rats to fix high blood pressure and to generate a hypertensive and diabetic BB/OK rat subline. After 8 backcrosses, the backcross parents were genetically analysed with the aid of 259 microsatellite markers to identify SHR genes causing blood pressure of 177 +/- 10 mmHg in this BB/OK rat subline. Read More

View Article

Download full-text PDF

Source

C-terminal region of hTPO is important for secretion and expression in insect cells.

Biochem Mol Biol Int 1999 May;47(5):729-33

R&D Center, Daewoong Pharmaceutical Co., Ltd., Sungnam, Korea.

Human thrombopoietin (hTPO) variant cDNAs truncated in the C-terminal regions of wild-type hTPO (332 amino acids) were constructed by PCR and expressed in Trichoplusia ni (Tn5) insect cells using a baculovirus expression system. Each variant, hTPO163 (amino acids 1-163), hTPO198 (1-198) and hTPO245 (1-245), was produced in insect cells with very low efficiency in comparison with wild-type hTPO. Immunoblot analysis showed that the predicted 20, 25 and 34 kDa molecular sizes corresponding to hTPO163, hTPO198 and hTPO245, respectively, were barely detected in culture medium and most of the proteins remained within the cell. Read More

View Article

Download full-text PDF

Source

Expression of the Na(+)-H+ exchanger isoform-1 and cyclooxygenases in human placentas: their implications in preeclampsia.

Biochem Mol Biol Int 1999 Apr;47(4):715-22

Department of Biochemistry, Faculty of Medicine, Kuwait University, Kuwait.

The sodium hydrogen exchanger isoform, NHE-1 plays an important role in electrolyte and water homeostasis. These functions are compromised in pregnancies complicated with preeclampsia. At present it is not known whether NHE-1 expression is altered during preeclampsia. Read More

View Article

Download full-text PDF

Source
April 1999
1 Read

Anti-tumor polysaccharide from the mycelium of liquid-cultured Agaricus blazei mill.

Biochem Mol Biol Int 1999 Apr;47(4):707-14

Graduate School of Science and Technology, Kobe University, Japan.

Anti-tumor active polysaccharide against Sarcoma 180 was isolated by DEAE-Sepharose CL-6B and Sepharose 4B column chromatography from the hot-water soluble fraction of the mycelium of liquid-cultured Agaricus blazei mill. This polysaccharide did not react with antibodies of anti-tumor polysaccharides such as lentinan, gliforan, and FIII-2-b which is one of anti-tumor polysaccharides from Agaricus blazei. Moreover, the analyses of 13C-NMR and GC-MS suggested that this polysaccharide was preliminarily glucomannan with a main chain of beta-1,2-linked D-mannopyranosyl residues and beta-D-glucopyranosyl-3-O-beta-D-glucopyranosyl residues as a side chain. Read More

View Article

Download full-text PDF

Source
April 1999
6 Reads

Isolation and characterization of an arginine ester hydrolase from Bothrops jararacussu venom which induces contractions of the isolated rat uterus.

Biochem Mol Biol Int 1999 Apr;47(4):699-706

Departamento de Bioquímica, Faculdade de Medicina, Universidade de São Paulo, Brazil.

The isolation and partial characterization of a serine protease with arginine ester hydrolase activity from Bothrops jararacussu snake venom are described. The purification procedure consisted of a gel filtration of the crude venom on Sephadex G-75 followed by an ion-exchange chromatography of the active fraction on DEAE-cellulose and a rechromatography on Bio-Rex 70 resin. The esterase fraction (DI-III), M(r) = 25,000 by SDS-PAGE, showed proteolytic activity on fibrinogen and casein. Read More

View Article

Download full-text PDF

Source

Polymerization of actin induced by a molar excess of ATP in a low salt buffer.

Authors:
T Ikkai H Kondo

Biochem Mol Biol Int 1999 Apr;47(4):691-7

Aichi Prefectural University of Fine Arts, Japan.

The polymerization of actin induced by dilution has previously been reported, where a 1000-fold molar excess of ATP over actin resulted when actin was diluted to 4.0 micrograms/ml in low salt buffer A (0.1 mM ATP, 0. Read More

View Article

Download full-text PDF

Source

Lipoprotein alterations, abdominal fat distribution and breast cancer.

Biochem Mol Biol Int 1999 Apr;47(4):681-90

Dept. of Clinical Biochemistry-Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina.

Plasma lipid profile and abdominal obesity have been associated with breast cancer risk, however published results have been inconsistent. To clarify these associations we studied lipid and lipoprotein alterations, obesity degree and body fat distribution, in 30 newly diagnosed breast cancer patients without treatment and 30 controls matched by age and menopausal status. Both pre and postmenopausal breast cancer patients presented higher body mass index, waist/hip ratio and insulin levels than their matched controls. Read More

View Article

Download full-text PDF

Source

Protein kinase C isoforms in the epidermal tissues of normal and postburn human skin.

Biochem Mol Biol Int 1999 Apr;47(4):673-9

Institute of Biochemistry, Chung Shan Medical College, Taichung, Taiwan.

Because the expression of the isoforms of protein kinase C (PKC) in human basal keratinocytes is not understood, the expression of PKC isoforms were screened in specimens of epidermal tissue from postburn skin and the normal locations for skin grafts in patients with second or higher degrees of flame injury. The expression of individual isoform was determined by Western blot technique. Only PKC alpha and zeta were detected in the epidermal tissues of normal and postburn skin and translocation occurred in PKC alpha. Read More

View Article

Download full-text PDF

Source

An NMR study of the structural basis of the wide range of pharmacological functions of acetylsalicylic acid.

Biochem Mol Biol Int 1999 Apr;47(4):665-71

School of Life Science, Wuhan University, P. R. China.

The interaction between acetylsalicylic acid (aspirin) and membrane was studied by NMR spectra. (1) NMR spectra showed acetylsalicylic acid did not insert into membrane; (2) 1H NMR spectrum recorded by spin-echo pulse sequence showed protons of the aromatic ring interacted with membrane; (3) the change of spin-lattice relaxation (T1) of 31P was ascribed to the association of acetylsalicylic acid to the polar head of lecithin; (4) the self-diffusion coefficient measured by pulsed field gradients NMR showed the mobility of acetylsalicylic acid was restricted by membrane and that acetylsalicylic acid changed membrane viscosity. Based on the results, the relationship between the interaction and the mechanism of the wide pharmacological functions of acetylsalicylic acid is discussed. Read More

View Article

Download full-text PDF

Source

Characterization of the human CR1 gene promoter.

Biochem Mol Biol Int 1999 Apr;47(4):655-63

School of Life Sciences, Chungbuk National University, Korea.

The human CR1 is a single chain membrane glycoprotein that is a member of the group of regulators of the complement activation system. In order to clarify the regulatory mechanisms of human CR1 gene expression, the 5'-flanking region of the human CR1 gene was isolated and its promoter was characterized. The CR1 expression was found to be transcriptionally up-regulated in HL60 cells by stimulation with DMSO. Read More

View Article

Download full-text PDF

Source

Lipid peroxidation induced by the Cu,Zn-superoxide dismutase and hydrogen peroxide system.

Authors:
O B Kwon J H Kang

Biochem Mol Biol Int 1999 Apr;47(4):645-53

Department of Genetic Engineering, Chongju University, Korea.

Cu,Zn-superoxide dismutase (SOD) can catalyze hydroxyl radical generation using H2O2 as a substrate. Lipid peroxidation induced by the Cu,Zn-SOD and H2O2 system was investigated. When linoleic acids micelles or phosphatidylcholine liposomes were incubated with Cu,Zn-SOD and H2O2, lipid peroxidation was gradually increased in a time-dependent manner. Read More

View Article

Download full-text PDF

Source