1,041,216 results match your criteria Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology[Journal]


Interleukin-18 serum levels in sepsis: Correlation with disease severity and inflammatory markers.

Cytokine 2019 Apr 16;120:22-27. Epub 2019 Apr 16.

Department of Anesthesiology and Intensive TherapyCare, 4th Military Hospital of Wroclaw, Weigla 5, 50-981 Wroclaw, Poland; Faculty of Health Science, Wroclaw Medical University, Wroclaw, Poland. Electronic address:

Purpose: Sepsis is defined as a life-threatening organ dysfunction caused by a dysregulated host response to infection and a syndrome shaped by pathogen and host factors with characteristic that evolve over time. The study was conducted to evaluate the prognostic and discriminative value of IL-18 assessment in comparison to PCT, CRP, WBC in early stage of sepsis and septic shock.

Methods: An observational and prospective study was conducted in the group of 40 ICU patients with diagnosis of sepsis or septic shock, serum PCT, IL-18, CRP and WBC measurements were performed on admission, and on the 2nd, 3rd and 5th therapy day. Read More

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http://dx.doi.org/10.1016/j.cyto.2019.04.003DOI Listing

Recipient ABCB1, donor and recipient CYP3A5 genotypes influence tacrolimus pharmacokinetics in liver transplant cases.

Pharmacol Rep 2019 Jan 11;71(3):385-392. Epub 2019 Jan 11.

Dept of Clinical Pharmacology and Therapeutics, Nizam's Institute of Medical Sciences, Hyderabad, India.

Background: Effective immunosuppression through optimization of trough levels tacrolimus reduces post-transplant mortality rate in liver transplant cases.

Methods: Meta-analysis was carried out to evaluate how donor/recipient CYP3A5 (n = 678) and recipient ABCB1 (n = 318) genotypes influence tacrolimus pharmacokinetics till one-month of transplantation.

Results: The donor CYP3A5*3/*3 genotype exhibited higher concentration/dose (C/D) ratio of tacrolimus in week 1 (mean difference: 65. Read More

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http://dx.doi.org/10.1016/j.pharep.2019.01.006DOI Listing
January 2019

Borrelia burgdorferi basic membrane protein A initiates proinflammatory chemokine storm in THP 1-derived macrophages via the receptors TLR1 and TLR2.

Biomed Pharmacother 2019 Apr 16;115:108874. Epub 2019 Apr 16.

Department of Biochemistry and Molecular Biology, Kunming Medical University, Kunming 650500, China.

Lyme disease, reffered to as Lyme borreliosis, is a tick-borne zoonotic disease caused by Borrelia burgdorferi spirochetes. Lyme arthritis, the most common, serious and harmful manifestation during the late stages of Lyme disease, is closely associated with the Borrelia burgdorferi basic membrane protein A (BmpA). Chemokines are also reported to have an important role in Lyme arthritis. Read More

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http://dx.doi.org/10.1016/j.biopha.2019.108874DOI Listing
April 2019
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Toxic, cytogenetic and antitumor evaluations of [6]-gingerol in non-clinical in vitro studies.

Biomed Pharmacother 2019 Apr 16;115:108873. Epub 2019 Apr 16.

Northeast Biotechnology Network (RENORBIO), Postgraduate Program in Biotechnology, Federal University of Piauí, Teresina, Piauí, 64049-550, Brazil; Postgraduate Program in Pharmaceutical Sciences, Laboratory of Genetic Toxicology, Federal University of Piauí, Teresina, Piauí, 64049-550, Brazil.

Gingerol - [6]-gingerol ((S)-5-hydroxy-1-(4-hydroxy-3-methoxyphenyl)-3-decanone; [6]-G) - is a phenolic compound with several pharmacological properties. Herein, the aim of the study was to evaluate the toxicogenic effects of [6]-G on Artemia salina nauplii, Allium cepa, HL-60 cell line and Sarcoma 180 (S-180) ascitic fluid cells.For toxic and genotoxic analysis, it was used [6]-G concentrations of 5, 10, 20 and 40 μg mL-1. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S07533322183570
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http://dx.doi.org/10.1016/j.biopha.2019.108873DOI Listing
April 2019
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Natural Variations at TIG1 Encoding a TCP Transcription Factor Contribute to Plant Architecture Domestication in Rice.

Mol Plant 2019 Apr 16. Epub 2019 Apr 16.

State Key Laboratory of Plant Physiology and Biochemistry, China Agricultural University, Beijing, 100193, China. Electronic address:

The modification of plant architecture was a crucial target in rice domestication and modern genetic improvement. Although a fraction of genes regulating rice plant architecture have been characterized, the molecular mechanisms underlying rice plant architecture domestication has not been elucidated. Here we show that the phenotype of inclined growth tiller in wild rice is controlled by a single dominant gene, TILLER INCLINED GROWTH 1 (TIG1), which is on chromosome 8 and encodes a TCP transcriptional activator. Read More

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http://dx.doi.org/10.1016/j.molp.2019.04.005DOI Listing

Oxidative stress-induced senescence markedly increases disc cell bioenergetics.

Mech Ageing Dev 2019 Apr 16. Epub 2019 Apr 16.

Department of Orthopedic Surgery, University of Pittsburgh, 200 Lothrop Street, Pittsburgh, PA, 15213, USA; Vascular Medicine Institute, University of Pittsburgh, Pittsburgh, PA, 15213, USA. Electronic address:

Cellular senescence is a phenotype characterized by irreversible growth arrest, chronic elevated secretion of proinflammatory cytokines and matrix proteases, a phenomenon known as senescence associated secretory phenotype (SASP). Biomarkers of cellular senescence have been shown to increase with age and degeneration of human disc tissue. Senescent disc cells in culture recapitulate features associated with age-related disc degeneration, including increased secretion of proinflammatory cytokines, matrix proteases, and fragmentation of matrix proteins. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00476374183019
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http://dx.doi.org/10.1016/j.mad.2019.04.006DOI Listing
April 2019
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RTN1-C is involved in high glucose-aggravated neuronal cell subjected to oxygen-glucose deprivation and reoxygenation injury via endoplasmic reticulum stress.

Brain Res Bull 2019 Apr 16. Epub 2019 Apr 16.

Department of Anesthesiology, the First Affiliated Hospital of Anhui Medical University, Hefei, 230032, China. Electronic address:

Background: Patients suffering from diabetes mellitus experience poor outcomes after ischemic stroke. RTN1-C, ER-associated proteins localized in endoplasmic reticulum (ER) membrane, plays an important role in ER stress-induced apoptosis and regulates cellular susceptibility to different apoptosis pathways. Overexpression of RTN1-C can aggravate cerebral ischemia/reperfusion injury (IRI). Read More

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http://dx.doi.org/10.1016/j.brainresbull.2019.04.010DOI Listing

Nanocrystalline cellulose: Preparation, physicochemical properties, and applications in drug delivery systems.

Int J Biol Macromol 2019 Apr 16. Epub 2019 Apr 16.

Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. Electronic address:

Cancer is the leading cause of death all over the world and chemotherapy is an important approach to fight cancer, however, there are many obstacles against successful cancer chemotherapy such as development of multidrug resistance, poor solubility of chemotherapeutic agents and adverse side effects to healthy tissues. An important strategy to overcome these obstacles, is the use of nanotechnology. In recent years, natural polymers such as cellulose and its nanoform structure, nanocrystalline cellulose (NCC), have attracted the interest of researchers in the field of nanotechnology and specially drug delivery systems, due to biocompatibility and biodegradability of NCC. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01418130193169
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http://dx.doi.org/10.1016/j.ijbiomac.2019.04.117DOI Listing
April 2019
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Reciprocal regulation of sulfite oxidation and nitrite reduction by mitochondrial sulfite oxidase.

Nitric Oxide 2019 Apr 16. Epub 2019 Apr 16.

Institute of Biochemistry, Department of Chemistry, University of Cologne, Cologne, 50674, Germany; Center for Molecular Medicine Cologne (CMMC), University of Cologne, Cologne, 50931, Germany. Electronic address:

The oxygen-independent nitrate-nitrite-nitric oxide (NO) pathway is considered as a substantial source of NO in mammals. Dietary nitrate/nitrite are distributed throughout the body and reduced to NO by the action of various enzymes. The intermembrane spaced (IMS), molybdenum cofactor-dependent sulfite oxidase (SO) was shown to catalyze such a nitrite reduction. Read More

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http://dx.doi.org/10.1016/j.niox.2019.04.004DOI Listing

Redox homeostasis in a rodent model of circadian disruption: Effect of melatonin supplementation.

Gen Comp Endocrinol 2019 Apr 16. Epub 2019 Apr 16.

Department of Biochemistry, University of Allahabad, Allahabad 211002, India. Electronic address:

Continuous light or dark photoperiods are the leading cause of disruption in the circadian rhythm of day-night cycle. The purpose of this study was to understand the cellular redox balance in a model of circadian disrupted rat model and determine the effect of melatonin supplementation. Young male Wistar rats were randomly divided into five groups (n = 4). Read More

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http://dx.doi.org/10.1016/j.ygcen.2019.04.016DOI Listing

Ubiquinone Biosynthetic Complexes in Prokaryotes and Eukaryotes.

Cell Chem Biol 2019 Apr;26(4):465-467

Department of Chemistry and Biochemistry and the Molecular Biology Institute, University of California, Los Angeles, Los Angeles, CA 90095, USA. Electronic address:

Ubiquinone (UQ) is a conserved polyprenylated lipid essential to cellular respiration. Two papers, one in this issue of Cell Chemical Biology (Hajj Chehade et al., 2019) and another in Molecular Cell (Lohman et al. Read More

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http://dx.doi.org/10.1016/j.chembiol.2019.04.005DOI Listing

SIRT6 Is Responsible for More Efficient DNA Double-Strand Break Repair in Long-Lived Species.

Cell 2019 Apr;177(3):622-638.e22

Department of Biology, University of Rochester, Rochester, NY 14627, USA. Electronic address:

DNA repair has been hypothesized to be a longevity determinant, but the evidence for it is based largely on accelerated aging phenotypes of DNA repair mutants. Here, using a panel of 18 rodent species with diverse lifespans, we show that more robust DNA double-strand break (DSB) repair, but not nucleotide excision repair (NER), coevolves with longevity. Evolution of NER, unlike DSB, is shaped primarily by sunlight exposure. Read More

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http://dx.doi.org/10.1016/j.cell.2019.03.043DOI Listing

Ginsenoside Rg1 attenuates hepatic insulin resistance induced by high-fat and high-sugar by inhibiting inflammation.

Eur J Pharmacol 2019 Apr 16. Epub 2019 Apr 16.

Department of Biochemistry and Molecular Biology, School of Basic Medicine, Kunming Medical University, Kunming, Yunnan, 650500, PR China; Yunnan Province Key Laboratory for Nutrition and Food Safety in Universities, PR China. Electronic address:

Ginsenoside Rg1 is the active ingredient of Chinese herbal medicine ginseng and sanqi, which has remarkable effects on anti-inflammation and anti-diabetes. In this study, we explored the molecular mechanism of ginsenoside Rg1 against diabetes in rat subjected to insulin resistance induced by high-fat and high-sugar (HFHS). Biochemical analysis revealed that ginsenoside Rg1 significantly decreased the serum levels of alanine transaminase, aspartate transaminase, alkaline phosphatase, total cholesterol, triglyceride, low-density lipoprotein and increased the serum levels of high-density lipoprotein, which indicated ginsenoside Rg1 improved the extent of hepatic steatosis. Read More

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http://dx.doi.org/10.1016/j.ejphar.2019.04.027DOI Listing

Direct Detection of Tissue-Resident Bacteria and Chronic Inflammation in the Bladder Wall of Postmenopausal Women with Recurrent Urinary Tract Infection.

J Mol Biol 2019 Apr 16. Epub 2019 Apr 16.

Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX, 75390, United States of America; Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, TX, 75390, United States of America; Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, TX, 75390, United States of America. Electronic address:

Urinary tract infections (UTIs) are the most commonly reported infections in adult women and have high rates of recurrence, especially in postmenopausal women. Recurrent UTI (RUTI) greatly reduces quality of life, places a significant burden on the healthcare system, and contributes to antimicrobial resistance. Because treatment of RUTI by long-term antibiotic therapy is often ineffective or poorly tolerated in elderly women, new therapies must be developed. Read More

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http://dx.doi.org/10.1016/j.jmb.2019.04.008DOI Listing

Laboratory intervention to improve the request of urinary albumin in primary care patients with arterial hypertension and financial implications.

Clin Biochem 2019 Apr 16. Epub 2019 Apr 16.

Department of Radiology, University of Missouri, Columbia, MO, USA.

Introduction: The request of Urinary albumin in primary care in Spain is insufficient to monitor patients with diabetes and hypertension (HTN). Our aim was to evaluate a strategy designed in consensus with general practitioners (GPs) to improve the request of urinary albumin in primary care patients with HTN according to guidelines, and to study its financial implications.

Materials And Methods: In a meeting with GPs, we decided that the Laboratory Information System (LIS) would automatically register the albumin-to-creatinine ratio (ACR) test in patients with HTN when the former had not been requested in the previous year. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00099120193006
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http://dx.doi.org/10.1016/j.clinbiochem.2019.04.012DOI Listing
April 2019
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Chemerin and Dermcidin in Human Milk and Their Alteration in Gestational Diabetes.

J Hum Lact 2019 Apr 19:890334419837523. Epub 2019 Apr 19.

5 Department of Medical Biochemistry and Clinical Biochemistry, Firat Hormones Research Group, Medical School, Firat University, Elazig, Turkey.

Background: Chemerin and dermcidin, which have antimicrobial properties, are molecules that are also related to insulin resistance and inflammation.

Research Aims: The aims were to determine the amounts of chemerin and dermcidin in the milk and blood of mothers with gestational diabetes, and to compare the amounts of chemerin and dermcidin in the milk and blood of mothers with and without diabetes.

Methods: This was a two-group nonrandomized longitudinal study with a convenience sampling of mothers without gestational diabetes ( n = 27) and mothers with gestational diabetes ( n = 26). Read More

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http://dx.doi.org/10.1177/0890334419837523DOI Listing

Structure and mechanism of TagA, a novel membrane-associated glycosyltransferase that produces wall teichoic acids in pathogenic bacteria.

PLoS Pathog 2019 Apr 19;15(4):e1007723. Epub 2019 Apr 19.

Department of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, United States of America.

Staphylococcus aureus and other bacterial pathogens affix wall teichoic acids (WTAs) to their surface. These highly abundant anionic glycopolymers have critical functions in bacterial physiology and their susceptibility to β-lactam antibiotics. The membrane-associated TagA glycosyltransferase (GT) catalyzes the first-committed step in WTA biosynthesis and is a founding member of the WecB/TagA/CpsF GT family, more than 6,000 enzymes that synthesize a range of extracellular polysaccharides through a poorly understood mechanism. Read More

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http://dx.doi.org/10.1371/journal.ppat.1007723DOI Listing

Impact of malaria and hepatitis B co-infection on clinical and cytokine profiles among pregnant women.

PLoS One 2019 19;14(4):e0215550. Epub 2019 Apr 19.

West African Centre for Cell Biology of Infectious Pathogens (WACCBIP), University of Ghana, Legon- Accra, Ghana.

Background: The overlap of malaria and chronic hepatitis B (CHB) is common in endemic regions, however, it is not known if this co-infection could adversely influence clinical and immunological responses. This study investigated these interactions in pregnant women reporting to antenatal clinics in Ghana.

Methods: Clinical parameters (hemoglobin, liver function biomarker, peripheral malaria parasitemia, and hepatitis B viremia) and cytokine profiles were assayed and compared across four categories of pregnant women: un-infected, mono-infected with Plasmodium falciparum (Malaria group), mono-infected with chronic hepatitis B virus (CHB group) and co-infected (Malaria+CHB group). Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0215550PLOS

Bioactive sphingolipid profile in a xenograft mouse model of head and neck squamous cell carcinoma.

PLoS One 2019 19;14(4):e0215770. Epub 2019 Apr 19.

Departments of Medicine and Biochemistry & the Stony Brook Cancer Center at Stony Brook University, Stony Brook, New York, United States of America.

The purpose of this study was to determine the profile of bioactive sphingolipids in xenograft mouse tissues of head and neck squamous cell carcinoma. We utilized UHPLC-MS/MS to determine the profile of full set of ceramides, sphingosine, and sphingosine 1-phosphate in this xenograft mouse model. The tissues isolated and investigated were from brain, lung, heart, liver, spleen, kidney, bladder, tumors and blood. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0215770PLOS

In vitro chondroprotective potential of Senna alata and Senna tora in porcine cartilage explants and their species differentiation by DNA barcoding-high resolution melting (Bar-HRM) analysis.

PLoS One 2019 19;14(4):e0215664. Epub 2019 Apr 19.

Excellent Center in Veterinary Bioscience, Chiang Mai University, Chiang Mai, Thailand.

Senna species and anthraquinone derivatives generated by these organisms, rhein and aloe-emodin, exert anti-inflammatory effects. These species present a similar morphology but produce different ingredients when they are used as medicinal products. In this study, a DNA barcoding- (Bar-) high-resolution melting (HRM) technique was developed using internal transcribed sequence 2 (ITS2) to differentiate between Senna alata and Senna tora as a result of significant differences in their melting profiles. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0215664PLOS

Adjunctive dabigatran therapy improves outcome of experimental left-sided Staphylococcus aureus endocarditis.

PLoS One 2019 19;14(4):e0215333. Epub 2019 Apr 19.

Department of Clinical Microbiology, Copenhagen University Hospital Rigshospitalet, Copenhagen, Denmark.

Background: Staphylococcus aureus is the most frequent and fatal cause of left-sided infective endocarditis (IE). New treatment strategies are needed to improve the outcome. S. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0215333PLOS

Optimization of the Process for Producing Biomass and Exopolysaccharide from the King Tuber Oyster Mushroom, Pleurotus tuber-regium (Agaricomycetes), for Biotechnological Applications.

Int J Med Mushrooms 2019 ;21(4):311-322

Microbiology Unit, Department of Pure and Applied Biology, Ladoke Akintola University of Technology, Ogbomosho, Oyo State, Nigeria.

The optimization of microbial growth for biotechnological purposes traditionally requires an approach that uses only one variable at a time, which has many drawbacks. This research used a completely randomized approach to optimize carbon and nitrogen nutrient requirements and growth factors (pH and temperature) for Pleurotus tuber-regium in order to optimally produce biomass and extracellular polysaccharide (EPS) in shake-flask cultures. An artificial neural network (ANN) module was used to simulate the fungus-growing process and hence determine optimal conditions. Read More

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http://dx.doi.org/10.1615/IntJMedMushrooms.2019030357DOI Listing
January 2019

Structure-Guided Drug Design of 6-Substituted Adenosine Analogues as Potent Inhibitors of Mycobacterium tuberculosis Adenosine Kinase.

J Med Chem 2019 Apr 19. Epub 2019 Apr 19.

Department of Biochemistry and Biophysics , Texas A&M University , College Station , Texas 77843 , United States.

Mycobacterium tuberculosis adenosine kinase (MtbAdoK) is an essential enzyme of Mtb and forms part of the purine salvage pathway within mycobacteria. Evidence suggests that the purine salvage pathway might play a crucial role in Mtb survival and persistence during its latent phase of infection. In these studies, we adopted a structural approach to the discovery, structure-guided design, and synthesis of a series of adenosine analogues that displayed inhibition constants ranging from 5 to 120 nM against the enzyme. Read More

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http://dx.doi.org/10.1021/acs.jmedchem.9b00020DOI Listing

Making it stick: A CURE designed to introduce students to the scientific process and the host response to foreign materials.

Biochem Mol Biol Educ 2019 Apr 19. Epub 2019 Apr 19.

Department of Biology, Penn State University, Lehigh Valley, Center Valley, Pennsylvania 18034.

Often overlooked in many molecular cell biology laboratory and cell culture courses, suspension cells represent an important aspect of molecular cell biology and cell culture. Most primary cell cultures and cell lines are adherent cells which grow in monolayers on surfaces. However, other cells such as hematopoietic cells, certain tumor cells, and cells of the immune system are suspension cells which are anchorage-independent which grow and divide in solution. Read More

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http://dx.doi.org/10.1002/bmb.21248DOI Listing

The interplay between microbiota-dependent metabolite trimethylamine N-oxide, Transforming growth factor β/SMAD signaling and inflammasome activation in chronic kidney disease patients: A new mechanistic perspective.

J Cell Biochem 2019 Apr 19. Epub 2019 Apr 19.

Internal Medicine Department, Faculty of Medicine, Tanta University, Tanta, Egypt.

Background: Chronic kidney disease (CKD) signifies a frequently life-threatening condition influencing kidney structure and function. Despite its irrefutable importance, its exact pathogenesis is not completely clarified. However, CKD is known to be associated with accumulated uremic toxins/metabolites, interstitial fibrosis, and systemic inflammation. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/jcb.28707
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http://dx.doi.org/10.1002/jcb.28707DOI Listing
April 2019
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Molecular mechanisms of adaptive evolution revealed by global selection for glyphosate resistance.

New Phytol 2019 Apr 19. Epub 2019 Apr 19.

Rothamsted Research, West Common, Harpenden, Hertfordshire, AL5 2JQ, UK.

The human-directed, global selection for glyphosate resistance in weeds has revealed a fascinating diversity of evolved resistance mechanisms, including herbicide sequestration in the vacuole, a rapid cell death response, nucleotide polymorphisms in the herbicide target (5-enolpyruvylshikimate-3-phosphate synthase, EPSPS) and increased gene copy number of EPSPS. For this latter mechanism, two distinct molecular genetic mechanisms have been observed, a tandem duplication mechanism and a large extrachromosomal circular DNA (eccDNA) that is tethered to the chromosomes and passed to gametes at meiosis. These divergent mechanisms have a range of consequences for the spread, fitness, and inheritance of resistance traits, and, particularly in the case of the eccDNA, demonstrate how evolved herbicide resistance can generate new insights in plant adaptation to contemporary environmental stress. Read More

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http://dx.doi.org/10.1111/nph.15858DOI Listing

Effect of electrochemotherapy with betulinic acid or cisplatin on regulation of heat shock proteins in metastatic human carcinoma cells in vitro.

Oncol Rep 2019 Apr 8. Epub 2019 Apr 8.

Department of Molecular and Cellular Biology, Faculty of Pharmacy with Division of Laboratory Diagnostics, Wroclaw Medical University, 50‑556 Wroclaw, Poland.

Betulinic acid (BTA) is naturally occurring triterpene that has received interest as a novel therapeutic substance with cytotoxicity towards a number of cancer cell lines. Despite the wide spectrum of biological and pharmacological effects, its effect may be limited its lipophobic properties. Therefore, strategies to improve the access of BTA to the cells are required to enhance the anticancer effects. Read More

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http://dx.doi.org/10.3892/or.2019.7103DOI Listing

Tomato fruit ripening factor NOR controls leaf senescence.

J Exp Bot 2019 Apr 19. Epub 2019 Apr 19.

Max Planck Institute of Molecular Plant Physiology, Am Mühlenberg, Potsdam-Golm, Germany.

NAC transcription factors (TFs) are important regulators of expressional reprogramming during plant development, stress responses, and leaf senescence. NAC TFs also play important roles in fruit ripening. In tomato (Solanum lycopersicum), one of the best characterized NACs involved in fruit ripening is NON-RIPENING (NOR), and the non-ripening (nor) mutation has been widely used to extend fruit shelf life in elite varieties. Read More

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https://academic.oup.com/jxb/advance-article/doi/10.1093/jxb
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http://dx.doi.org/10.1093/jxb/erz098DOI Listing
April 2019
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Lysyl oxidase-like 2 (LOXL2) depletion is protective in age-associated vascular stiffening.

Am J Physiol Heart Circ Physiol 2019 Apr 19. Epub 2019 Apr 19.

ACCM, Johns Hopkins University, United States.

Vascular stiffening and its sequelae are major causes of morbidity and mortality in the elderly. The increasingly accepted concept of "smooth muscle cell (SMC) stiffness syndrome" along with matrix deposition has emerged in vascular biology to account for the mechanical phenotype of arterial aging, but the molecular targets remain elusive. In this study, using an unbiased proteomic analysis, we identified lysyl oxidase-like 2 (LOXL2) as a critical SMC mediator for age-associated vascular stiffening. Read More

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https://www.physiology.org/doi/10.1152/ajpheart.00670.2018
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http://dx.doi.org/10.1152/ajpheart.00670.2018DOI Listing
April 2019
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Treatment with apolipoprotein A1 protects mice against doxorubicin-induced cardiotoxicity in a scavenger receptor class B type I dependent manner.

Am J Physiol Heart Circ Physiol 2019 Apr 19. Epub 2019 Apr 19.

Biochemistry and Biomedical Sciences; and Thrombosis and Atherosclerosis Research Institute, McMaster University, Canada.

Doxorubicin, an agent used to treat a variety of cancers, is cardiotoxic by triggering cardiomyocyte apoptosis. We previously showed that treating cultured cardiomyocytes with human high density lipoprotein in vitro or transgenic overexpression of human apolipoprotein A1, its main structural protein protects against doxorubicin induced cardiomyocyte apoptosis in a manner dependent on the scavenger receptor class B type I [Durham (2018) 314: H31-H44]. This was due to high density lipoprotein induced activation of Akt signaling in cardiomyocytes. Read More

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https://www.physiology.org/doi/10.1152/ajpheart.00432.2018
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http://dx.doi.org/10.1152/ajpheart.00432.2018DOI Listing
April 2019
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CARDIAC INVOLVEMENT IN Beta-THALASSAEMIA: Current treatment strategies.

Postgrad Med 2019 Apr 19. Epub 2019 Apr 19.

e Department of Medicine , Sultan Qaboos University Hospital , Muscat , Oman.

Despite the advances in the management of thalassaemia major, heart disease remains the leading cause of mortality in patients afflicted with this disorder. Cardiac involvement in thalassaemia encompasses a spectrum of disorders including myocardial dysfunction, arrhythmias, pulmonary hypertension and peripheral vascular disease. Although cardiac siderosis (accumulation of iron in cardiac myocytes) as a consequence of repeated blood transfusions is deemed to be the main aetiologic factor for myocardial dysfunction in transfusion dependent patients, the significance of other pathophysiologic mechanisms is being increasingly recognized especially in non-transfusion dependent patients. Read More

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https://www.tandfonline.com/doi/full/10.1080/00325481.2019.1
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http://dx.doi.org/10.1080/00325481.2019.1608071DOI Listing
April 2019
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Engineering Order and Cooperativity in a Disordered Protein.

Biochemistry 2019 Apr 19. Epub 2019 Apr 19.

Structural disorder in proteins arises from a complex interplay between weak hydrophobicity and unfavorable electrostatic interactions. The extent to which hydrophobic effect contributes to the unique and compact native state of proteins is however confounded by large compensation between multiple entropic and energetic terms. Here we show that protein structural order and cooperativity arise as emergent properties upon hydrophobic substitutions in a disordered system with non-intuitive effects on folding and function. Read More

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http://dx.doi.org/10.1021/acs.biochem.9b00182DOI Listing

Could Hepatitis B Vaccine Act as An Adjuvant to Lower Risk and Relapses of Cancer?

Clin Exp Pharmacol Physiol 2019 Apr 19. Epub 2019 Apr 19.

Department of Neurosurgery, Acibadem University, İstanbul, Turkey.

In this review article, we hypothesize that Hepatitis B Virus Vaccine (HBV-V) and certain antigens of Hepatitis B Virus (HBV)-could act as anticancer immunoadjuvants in addition to their role of preventing HBV-associated liver cancer. Evidence suggests that in animal breast cancer and melanoma models, combining hepatitis B-surface antigen (HBsAg) with other cancer antigens resulted in enhanced antitumor activity. HBsAg shares antigenic mimicry with healthy and malignant cells including squamous epithelia, thymic epithelia, bladder- and colon cancer cells. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1111/1440-1681.13
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http://dx.doi.org/10.1111/1440-1681.13096DOI Listing
April 2019
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The association between clinical and laboratory parameters in thyroid disease and nonthyroidal illness in young women.

Eur Rev Med Pharmacol Sci 2019 Apr;23(7):2950-2959

Department of Biochemistry and Clinical Chemistry, Medical University of Warsaw, Warsaw, Poland.

Objective: Evidence from epidemiological and clinical studies strongly suggest that young women in the preconception period are a group at risk for thyroid disorders which may lead to further aggravation of pre-existing chronic thyroid disease and complications during pregnancy.

Materials And Methods: This paper is a literature review focusing on articles published in English between 2014-2017 searched in Medline database using terms 'young women', 'subclinical hyperthyroidism', 'subclinical hypothyroidism', 'nonthyroidal illness syndrome', 'obesity', 'depression'.

Conclusions: Clinical assessment of young female patient including that of obtaining full medical history with a focus on the perceived changes in appearance, psychological symptoms and menstrual irregularities supported by laboratory tests indicative of metabolic status and characteristic changes in thyroid functions, may be the key to well-reasoned and justified individual therapeutic decisions. Read More

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http://dx.doi.org/10.26355/eurrev_201904_17575DOI Listing

Influence of Incubation Time and Incubation Tube on the Cytokine and Growth Factor Concentrations of Autologous Conditioned Serum in Horses.

J Equine Vet Sci 2019 Apr 8;75:30-34. Epub 2019 Jan 8.

Equine Clinic, Free University of Berlin, Berlin, Germany.

Autologous conditioned serum (ACS) is commonly used for the treatment of human and equine osteoarthritis (OA). The objective of this study was to investigate the influence of incubation time and incubation tube on concentrations of interleukin-1 receptor antagonist (IL-1ra), interleukin-1 beta (IL-1β), and insulin-like growth factor-1 (IGF-1) in ACS of horses with and without OA. Blood samples of 7 control horses and 6 horses with naturally occurring OA were obtained. Read More

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http://dx.doi.org/10.1016/j.jevs.2018.12.015DOI Listing

A Facile, Versatile, and Highly Efficient Strategy for Peroxynitrite Bioimaging Enabled by Formamide Deformylation.

Anal Chem 2019 Apr 19. Epub 2019 Apr 19.

Peroxynitrite (ONOO-) is attracting increasing attention due to its involvement in multiple facets of pathophysiological processes. However, ONOO- bioimaging are still challenging due to (1) the lack of highly specific reaction triggers, (2) the tedious and low-yielding synthesis of current sophisticated probes, and (3) the lack of availability of a versatile chemical strategy. To address these challenges, based on the amine formylation/deformylation chemistry, we have developed a novel strategy for ONOO- bioimaging. Read More

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http://dx.doi.org/10.1021/acs.analchem.9b01175DOI Listing

[Long non-coding RNA and cervical cancer].

Sheng Wu Gong Cheng Xue Bao 2019 Apr;35(4):598-606

Institute of Biochemistry and Molecular Biology, Guangdong Medical University, Zhanjiang 524023, Guangdong, China.

Long non-coding RNAs (lncRNAs) are members of RNA that are structurally similar to mRNA. They cannot encode proteins because they do not have a conserved open reading frame. LncRNAs were once regarded as abnormalities or noises or without any biological function after gene transcription. Read More

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http://dx.doi.org/10.13345/j.cjb.180493DOI Listing

Decreased cortisol response to low-dose Synacthen test in pediatric patients with type 1 diabetes.

J Diabetes 2019 Apr 19. Epub 2019 Apr 19.

1st Department of Pediatrics, School of Medicine, Faculty of Health Science, Aristotle University of Thessaloniki, Hippokration General Hospital, Thessaloniki.

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https://onlinelibrary.wiley.com/doi/abs/10.1111/1753-0407.12
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http://dx.doi.org/10.1111/1753-0407.12935DOI Listing
April 2019
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A gamma-thionin protein from apple, MdD1, is required for defense against S-RNase-induced inhibition of pollen tube prior to self / non-self recognition.

Plant Biotechnol J 2019 Apr 18. Epub 2019 Apr 18.

Laboratory of Fruit Cell and Molecular Breeding, China Agricultural University, Beijing, 100193, China.

Apple exhibits S-RNase-mediated self-incompatibility. Although the cytotoxic effect of S-RNase inside the self-pollen tube has been studied extensively, the underlying defense mechanism in pollen tube in Rosaceae remains unclear. On exposure to stylar S-RNase plant defense responses are activated in the pollen tube, however how these are regulated is currently poorly understood. Read More

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http://dx.doi.org/10.1111/pbi.13131DOI Listing

Biochemical diversity of glycosphingolipid biosynthesis as a driver of Coccolithovirus competitive ecology.

Environ Microbiol 2019 Apr 18. Epub 2019 Apr 18.

Department of Marine and Coastal Sciences, Rutgers University, New Brunswick, NJ 08901, USA.

Coccolithoviruses (EhVs) are large, double-stranded DNA-containing viruses that infect the single-celled, marine coccolithophore Emiliania huxleyi. Given the cosmopolitan nature and global importance of E. huxleyi as a bloom-forming, calcifying, photoautotroph, E. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1111/1462-2920.14
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http://dx.doi.org/10.1111/1462-2920.14633DOI Listing
April 2019
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CO -fixing liquid droplets: towards a dissection of the microalgal pyrenoid.

Traffic 2019 Apr 18. Epub 2019 Apr 18.

School of Biological Sciences, Nanyang Technological University, Singapore, Singapore.

CO enters the biosphere via the slow, oxygen-sensitive carboxylase, Rubisco. To compensate, most microalgae saturate Rubisco with its substrate gas through a carbon dioxide concentrating mechanism. This strategy frequently involves compartmentalization of the enzyme in the pyrenoid, a non-membrane enclosed compartment of the chloroplast stroma. Read More

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http://dx.doi.org/10.1111/tra.12650DOI Listing

Synthesis, Characterization, and Electron-Transfer Properties of Fc-BODIPY-C60 NIR Absorbing Triads: Are Catecholopyrrolidine-Linked Fullerenes a Good Architecture to Facilitate Electron-Transfer?

Chemistry 2019 Apr 18. Epub 2019 Apr 18.

University of Manitoba, Department of Chemistry, 360 Parker Building , R3T 2N2, Winnipeg, CANADA.

A series of covalent Fc-BODIPY-C60 triads with the ferrocene groups conjugated to the BODIPY π-system and the fullerene acceptor linked at the boron hub via a common catecholpyrrolidine bridge were prepared and characterized by spectroscopy, and, for one of the derivatives, X-ray crystallography. Redox processes of the new compounds were investigated by electrochemistry and spectroelectrochemistry. DFT calculations indicate that the HOMO in all triads was delocalized between ferrocene and BODIPY π-system, the LUMO was always fullerene-centered, and the catechol-centered occupied orbital was close in energy to the HOMO. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/chem.2019012
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http://dx.doi.org/10.1002/chem.201901225DOI Listing
April 2019
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Mitochondria-coupled glucose phosphorylation develops after birth to modulate H O release and calcium handling in rat brain.

J Neurochem 2019 Apr 18. Epub 2019 Apr 18.

Laboratory of Bioenergetics and Mitochondrial Physiology, Institute of Medical Biochemistry Leopoldo de Meis, Center for Health Sciences, Federal University of Rio de Janeiro, Rio de Janeiro, RJ-21941-902, Brazil.

The adult brain is a high-glucose and oxygen-dependent organ, with an extremely organized network of cells and large energy-consuming synapses. To reach this level of organization, early stages in development must include an efficient control of cellular events and regulation of intracellular signaling molecules and ions such as hydrogen peroxide (H O ) and calcium (Ca ), but in cerebral tissue, these mechanisms of regulation are still poorly understood. Hexokinase (HK) is the first enzyme in the metabolism of glucose and, when bound to mitochondria (mtHK), it has been proposed to have a role in modulation of mitochondrial H O generation and Ca handling. Read More

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http://dx.doi.org/10.1111/jnc.14705DOI Listing

Metal Binding Antimicrobial Peptides in Nanoparticle Bio-functionalization: New Heights in Drug Delivery and Therapy.

Probiotics Antimicrob Proteins 2019 Apr 18. Epub 2019 Apr 18.

Biochemistry Department, Faculty of Science, Ege University, 35100, Bornova, Izmir, Turkey.

Peptides are considered very important due to the diversity expressed through their amino acid sequence, structure variation, large spectrum, and their essential role in biological systems. Antimicrobial peptides (AMPs) emerged as a potent tool in therapy owing to their antimicrobial properties but also their ability to trespass the membranes, specificity, and low toxicity. They comprise a variety of peptides from which specific amino acid-rich peptides are of interest to the current review due to their features in metal interaction and cell penetration. Read More

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http://link.springer.com/10.1007/s12602-019-09546-5
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http://dx.doi.org/10.1007/s12602-019-09546-5DOI Listing
April 2019
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Silicon-mediated role of 24-epibrassinolide in wheat under high-temperature stress.

Environ Sci Pollut Res Int 2019 Apr 18. Epub 2019 Apr 18.

Plant Physiology and Biochemistry Section, Department of Botany, Aligarh Muslim University, Aligarh, 202002, India.

High temperature poses a severe extortion to productivity of many crops like wheat. Therefore, well documented roles of brassinosteroid (BR) and silicon (Si) in terms of abiotic stress tolerance, the current study was designed to evaluate the response of wheat (Triticum aestivum L. Var. Read More

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http://link.springer.com/10.1007/s11356-019-04938-0
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http://dx.doi.org/10.1007/s11356-019-04938-0DOI Listing
April 2019
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Genetic Determinants of Weight Loss After Bariatric Surgery.

Obes Surg 2019 Apr 17. Epub 2019 Apr 17.

Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3A, 2200, Copenhagen N, Denmark.

Background: The weight loss after bariatric surgery shows considerable individual variation. Twin studies of response to dietary interventions and studies of bariatric surgery patients suggest that genetic differences may play a role. This study aimed to examine the effect of three genetic risk scores on the inter-individual variation in excess body mass index loss (EBMIL) after Roux-en-Y gastric bypass. Read More

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http://link.springer.com/10.1007/s11695-019-03878-5
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http://dx.doi.org/10.1007/s11695-019-03878-5DOI Listing
April 2019
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Ultra-High Resolution Elemental/Isotopic Mass Spectrometry (m/Δm > 1,000,000): Coupling of the Liquid Sampling-Atmospheric Pressure Glow Discharge with an Orbitrap Mass Spectrometer for Applications in Biological Chemistry and Environmental Analysis.

J Am Soc Mass Spectrom 2019 Apr 18. Epub 2019 Apr 18.

Department of Chemistry, Clemson University, Clemson, SC, 29634, USA.

Many fundamental questions of astrophysics, biochemistry, and geology rely on the ability to accurately and precisely measure the mass and abundance of isotopes. Taken a step further, the capacity to perform such measurements on intact molecules provides insights into processes in diverse biological systems. Described here is the coupling of a combined atomic and molecular (CAM) ionization source, the liquid sampling-atmospheric pressure glow discharge (LS-APGD) microplasma, with a commercially available ThermoScientific Fusion Lumos mass spectrometer. Read More

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http://dx.doi.org/10.1007/s13361-019-02183-wDOI Listing

The use of crevicular fluid to assess markers of inflammation and angiogenesis, IL-17 and VEGF, in patients with solid tumors receiving zoledronic acid and/or bevacizumab.

Support Care Cancer 2019 Apr 17. Epub 2019 Apr 17.

1st Oncology Department, 'Metropolitan Hospital', 9 Ethnarxou Makariou Avenue, Piraeus, Greece.

Purpose: Crevicular fluid was used to assess interleukin-17 (IL-17) and vascular endothelial growth factor (VEGF) in cancer patients receiving zoledronic acid and/or bevacizumab. The markers were also assessed in the serum.

Methods: Twenty-five patients were included and comprised three groups: patients who received zoledronic acid (n = 9), patients who received bevacizumab (n = 9), and patients who received zoledronic acid combined with bevacizumab (n = 5). Read More

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http://dx.doi.org/10.1007/s00520-019-04793-2DOI Listing

Bone marrow pericyte dysfunction in individuals with type 2 diabetes.

Diabetologia 2019 Apr 17. Epub 2019 Apr 17.

Bristol Heart Institute, University of Bristol, Bristol Royal Infirmary, Level 7, Upper Maudlin Street, Bristol, BS2 8HW, UK.

Aims/hypothesis: Previous studies have shown that diabetes mellitus destabilises the integrity of the microvasculature in different organs by damaging the interaction between pericytes and endothelial cells. In bone marrow, pericytes exert trophic functions on endothelial cells and haematopoietic cells through paracrine mechanisms. However, whether bone marrow pericytes are a target of diabetes-induced damage remains unknown. Read More

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http://dx.doi.org/10.1007/s00125-019-4865-6DOI Listing

Rv1075c of Mycobacterium tuberculosis is a GDSL-Like Esterase and is Important for Intracellular Survival.

J Infect Dis 2019 Apr 18. Epub 2019 Apr 18.

Department of Microbiology, Immunology, and Biochemistry, University of Tennessee Health Science Center, Memphis, TN.

Mycobacterium tuberculosis (Mtb) lipid metabolism pathways facilitate access to carbon and energy sources for Mtb during infection. A Mtb gene, Rv1075c, was annotated as a conserved hypothetical protein. We have identified that Rv1075c amino acid sequence shares similarities to other bacterial lipase/esterases. Read More

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http://dx.doi.org/10.1093/infdis/jiz169DOI Listing
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