3,246 results match your criteria Biochemistry and cell biology = Biochimie et biologie cellulaire[Journal]


A prospective on multiple biological activities of lactoferrin contributing into piglet welfare.

Biochem Cell Biol 2020 Jun 27. Epub 2020 Jun 27.

Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China ;

Piglets, especially for weaning piglets, due to rapid growth, physiological immaturity and gradual reduction of maternal antibodies, showed a lower immunity level and higher morbidity, which affects seriously growth performance and economic benefits. It is important that the piglets are how to adapt to nutrient digestion and absorption, and to develop the intestinal function and the colonization of gut microbiota during early stage as soon as possible. Lactoferrin is a natural glycoprotein polypeptide that is differentiated from the transferrin family. Read More

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http://dx.doi.org/10.1139/bcb-2020-0078DOI Listing

Minireview: Lactoferrin, a versatile natural antimicrobial glycoprotein which modulates host innate immunity.

Biochem Cell Biol 2020 Jun 25. Epub 2020 Jun 25.

Ghent University Faculty of Veterinary Medicine, 86797, Merelbeke, Belgium ;

Lactoferrin is a multifunctional protein found in the secretions of mammals. The antimicrobial activity of lactoferrin was the first to be discovered and was assumed to be solely dependent on its iron-chelating ability. However, lactoferrin has been reported to display proteolytic activity towards bacterial virulence factors and to modulate the host defence by stimulating the immune system and balancing pathogen-induced inflammation. Read More

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http://dx.doi.org/10.1139/bcb-2020-0080DOI Listing

MiR-27b-3p suppresses glioma development via targeting YAP1.

Biochem Cell Biol 2020 Jun 22:466-473. Epub 2020 Jun 22.

Department of Neurosurgery, Zhongda Hospital, Southeast University, Nanjing, Jiangsu 210009, P.R. China.

Previous studies have reported that miRNAs are involved in the progression of glioma, and that miR-27b-3p is involved in a variety of cancers. However, whether miR-27b-3p has a role in glioma is still unknown. Here, we demonstrated that miR-27b-3p is downregulated in glioma, and this is associated with the development of glioma. Read More

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http://dx.doi.org/10.1139/bcb-2019-0300DOI Listing

Binding of lactoferrin to the surface of low-density lipoproteins modified by myeloperoxidase prevents intracellular cholesterol accumulation by human blood monocytes.

Biochem Cell Biol 2020 Jun 16. Epub 2020 Jun 16.

Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency , Malaya Pirogovskaya str., 1a , Moscow, Russian Federation , 119435 ;

Myeloperoxidase (MPO) is unique heme-containing peroxidase which can catalyze formation of hypochlorous acid (HOCl). Strong interaction of MPO with low-density lipoproteins (LDL) promotes proatherogenic modification of LDL by HOCl. The so-called MPO-modified LDL (Mox-LDL) accumulates in macrophages with formation of foam cells, which is the pathognomic symptom of atherosclerosis. Read More

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http://dx.doi.org/10.1139/bcb-2020-0141DOI Listing

Comparative effect of bovine buttermilk, whey, and lactoferrin on the innate immunity receptors and oxidative status of intestinal epithelial cells.

Biochem Cell Biol 2020 Jun 13. Epub 2020 Jun 13.

University of Zaragoza Faculty of Veterinary, 88203, Producción Animal y Ciencia de los Alimentos, Zaragoza, Aragón, Spain ;

Milk contains active molecules with important functional properties as the defensive proteins; among them are the whey protein lactoferrin and proteins of the milk fat globule membrane (MFGM) present in buttermilk. The aim of this study has been to investigate the effect of lactoferrin, whey and buttermilk as modulators of intestinal innate immunity and oxidative stress on intestinal epithelial cells, to evaluate its potential use for the development of functional foods. Innate immune Toll-like receptors (TLR2, TLR4, and TLR9) mRNA expression, lipid peroxidation (MDA+4-HDA) and protein carbonyl levels were analyzed in enterocyte-like Caco-2/TC7 cells treated for 24 hours with different concentrations of lactoferrin, whey or buttermilk. Read More

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http://dx.doi.org/10.1139/bcb-2020-0121DOI Listing

Inhibition of long noncoding RNA MALAT1 suppresses high glucose-induced apoptosis and inflammation in human umbilical vein endothelial cells by suppressing the NF-κB signaling pathway.

Biochem Cell Biol 2020 Jun 5. Epub 2020 Jun 5.

Pingxiang People's Hospital, Pingxiang, China ;

The study aimed to investigate the expression of long noncoding RNA (lncRNA) MALAT1 in high glucose (HG)-induced human vascular endothelial cells (HUVECs) and the role of MALAT1 in the apoptosis of HG-induced HUVECs. HUVECs were cultured and induced via 25 mmol/L HG. After that, HUVECs were transfected with MALAT1 siRNA. Read More

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http://dx.doi.org/10.1139/bcb-2019-0403DOI Listing

Mistranslating tRNA identifies a deleterious S213P mutation in the Saccharomyces cerevisiae eco1-1 allele.

Biochem Cell Biol 2020 May 30. Epub 2020 May 30.

University of Western Ontario, 6221, Biochemistry, London, Ontario, Canada ;

Mistranslation occurs when an amino acid not specified by the standard genetic code is incorporated during translation. Since the ribosome does not read the amino acid, tRNA variants aminoacylated with a non-cognate amino acid or containing a non-cognate anticodon dramatically increase the frequency of mistranslation. In a systematic genetic analysis, we identified a suppression interaction between tRNASerUGG, G26A, which mistranslates proline codons by inserting serine, and eco1-1, a temperature sensitive allele of the gene encoding an acetyltransferase required for sister chromatid cohesion. Read More

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http://dx.doi.org/10.1139/bcb-2020-0151DOI Listing

Lactoferrin levels in cervical fluid from in vitro fertilization (IVF) patients. Correlation with IVF parameters.

Biochem Cell Biol 2020 May 31. Epub 2020 May 31.

Universidad Nacional de Rosario, 28237, Clinical Biochemistry, Rosario, Argentina ;

Since our previous results suggest that LF might have roles in the reproductive process and its levels might change in the female tract as a response to various factors, the aim of this investigation was to assess whether LF levels in cervical secretions correlate with reproductive parameters from in vitro fertilization (IVF) patients. Cervical fluid samples were obtained from 34 women under 40 years old enrolled for assisted reproduction techniques and LF concentration was measured. The mean total protein concentration in all cervical fluid samples was 842. Read More

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http://dx.doi.org/10.1139/bcb-2020-0098DOI Listing

New Insights into the Systemic Effects of Oral Lactoferrin: Transcriptome Profiling.

Biochem Cell Biol 2020 May 30. Epub 2020 May 30.

UTHealth McGovern Medical School , McGovern Medical School , Houston, Texas, United States , 77030 ;

The immunomodulatory nature of lactoferrin (LF) derives from its ability to bridge innate and adaptive immunity in obtaining physiological equilibrium. LF is an attractive molecule for treatment of diseases that compromise immune homeostasis. Oral delivery is a preferable method for LF administration; however, its bioavailability is affected by protein degradation and absorption. Read More

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http://dx.doi.org/10.1139/bcb-2020-0069DOI Listing

The N-glycans of lactoferrin: More than just a sweet decoration.

Biochem Cell Biol 2020 May 28. Epub 2020 May 28.

Leibniz Institute for Farm Animal Biology (FBN), Institute of Reproductive Biology , Wilhelm-Stahl-Allee 2 , Dummerstorf, Germany , 18196 ;

Nearly all extracellular proteins undergo posttranslational modification with sugar chains during their transit through the endoplasmic reticulum and the Golgi apparatus. These "sweet" modifications not only influence the activity of its carrier protein, but they themselves often have bioactivity, independent of the carrier function. Lactoferrin belongs to the group of glycoproteins and is modified with several different N-glycans. Read More

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http://dx.doi.org/10.1139/bcb-2020-0106DOI Listing

FOXD3 inhibits cell proliferation, migration and invasion in nasopharyngeal carcinoma through regulation of the PI3K/Akt pathway.

Biochem Cell Biol 2020 May 27. Epub 2020 May 27.

Zhejiang Cancer Hospital, 89680, Anesthesiology, Hangzhou, Zhejiang, China ;

FOXD3 has been found previously to positively regulate miR-26b, a tumor inhibitor of nasopharyngeal carcinoma (NPC). However, its precise function and associated mechanism of action in NPC has yet been investigated. In this study, FOXD3 mRNA and protein expression was evaluated using RT-qPCR, western blotting and immunohistochemistry (IHC). Read More

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http://dx.doi.org/10.1139/bcb-2020-0011DOI Listing

YAP confers resistance to vandetanib in medullary thyroid cancer.

Biochem Cell Biol 2020 Jun 25;98(3):443-448. Epub 2020 May 25.

Department of General Surgery, Jiaxing Maternity and Child Health Care Hospital, No. 2468 Central East Road, Nanhu District, Jiaxing 314000, Zhejiang, China.

Medullary thyroid cancer (MTC) is the third most common thyroid cancer. (Rearranged in Transformation) gene mutations are considered as one of the major drivers of MTC. Vandetanib suppresses RET activity, and has shown promise in clinical trials. Read More

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http://dx.doi.org/10.1139/bcb-2019-0354DOI Listing

Circ_BMP2K enhances the regulation of miR-455-3p on its target gene SUMO1 to inhibit the activation of cardiac fibroblasts.

Biochem Cell Biol 2020 May 15. Epub 2020 May 15.

First Hospital of Shanxi Medical University, 105862, Department of Cardiology , 85# Jiefang South Road, Yingze District, Taiyuan 030001, Shanxi Province, P.R. China. , Taiyuan, China , 030001 ;

It has been found that some circular RNAs (circRNA) are abnormally expressed in the process of myocardial fibrosis, but the mechanism of them is still unknown. In the process of inducing cardiac fibroblast (CF) activation with TGF-β1 or Ang II, circRNA circ_BMP2K and miR-455-3p expression was significantly inhibited, and SUMO1 expression was promoted. Dual luciferase reporter gene assay, RIP and pull down assay showed that miR-455-3p bound with circ_BMP2K directly, and they promoted each other's expression. Read More

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http://dx.doi.org/10.1139/bcb-2019-0381DOI Listing
May 2020
2.152 Impact Factor

Lactoferrin Reduces Mycobacterial Trehalose 6,6'-dimycolate Induced M1-type Inflammation and Permits Fluoroquinolone Entry to Granulomas.

Biochem Cell Biol 2020 May 13. Epub 2020 May 13.

UTHealth McGovern Medical School , McGovern Medical School , Houston, Texas, United States , 77030 ;

Primary Mycobacterium tuberculosis (Mtb) infection results in the formation of a densely packed granulomatous response that essentially limits entry and efficacy of immune effector cells. Furthermore, the physical nature of the granuloma does not readily permit entry of therapeutic agents to sites where organisms reside. The Mtb cell wall mycolic acid, trehalose 6,6'-dimycolate (TDM), is a physiologically-relevant molecule to model macrophage mediated events during establishment of the tuberculosis-induced granuloma pathogenesis. Read More

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http://dx.doi.org/10.1139/bcb-2020-0057DOI Listing
May 2020
2.152 Impact Factor

Chondroitin polymerizing factor (CHPF) contributes to malignant proliferation and migration of hepatocellular carcinoma cells.

Biochem Cell Biol 2020 Jun 8;98(3):362-369. Epub 2020 May 8.

Department of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China.

Hepatocellular carcinoma (HCC) is one of the most common malignant tumors in the human digestive system, and has been recognized as a serious threat to public health worldwide. This study explored the role of chondroitin polymerizing factor (CHPF) in the development and metastasis of HCC. Immunohistochemistry analysis was performed to detect CHPF expression in HCC tissues and para-carcinoma tissues. Read More

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http://dx.doi.org/10.1139/bcb-2019-0227DOI Listing

Tetramethylpyrazine ameliorates hepatic fibrosis through autophagy-mediated inflammation.

Biochem Cell Biol 2020 Jun 8;98(3):327-337. Epub 2020 May 8.

Department of Hepatobiliary Surgery, The First Affiliated Hospital of Guangxi Medical University, No. 6, Shuangyong Road, Nanning 530021, P.R. China.

Background: Imbalanced immune response and hepatic fibrosis are key factors related to the progression of chronic liver diseases. Tetramethylpyrazine (TMP), a natural alkaloid, has been widely used for treating liver injury. In this study, we explored the effect of TMP on hepatic fibrosis and the related mechanisms regulating autophagy. Read More

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http://dx.doi.org/10.1139/bcb-2019-0059DOI Listing

Long non-coding RNA DSCAM-AS1 upregulates USP47 expression through sponging miR-101-3p to accelerate osteosarcoma progression.

Biochem Cell Biol 2020 May 7. Epub 2020 May 7.

China-Japan Union Hospital of Jilin University, 74569, Changchun, China , 130031 ;

Osteosarcoma (OS) originating from the mesenchymal is one of the most common bone invasive tumors with an extremely high mortality rate. Previous studies have reported that long non-coding RNAs (lncRNAs) play essential roles in the tumorigenesis and progression of a multitude of human cancers. LncRNA DSCAM-AS1 has been reported to be an oncogenic gene in many cancers. Read More

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http://dx.doi.org/10.1139/bcb-2020-0031DOI Listing

Long noncoding RNA KCNQ1OT1 is correlated with human breast cancer cell development through inverse regulation of hsa-miR-107.

Biochem Cell Biol 2020 Jun 7;98(3):338-344. Epub 2020 May 7.

Mammary gland thyroid Surgery, Affiliated Hospital of Jining Medical University, Jining, 272000, Shandong, China.

In this work, we investigated the expression pattern and regulatory function of long noncoding RNA (lncRNA) KCNQ1 opposite strand/antisense transcript 1 (KCNQ1OT1) in breast cancer. We found that KCNQ1OT1 was significantly upregulated in breast cancer cell lines. In lentiviral-transduced BT-549 and HCC1599 cells, KCNQ1OT1 knockdown impaired cancer cell functions, including in vitro proliferation and migration, and in vivo transplant growth. Read More

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http://dx.doi.org/10.1139/bcb-2019-0271DOI Listing
June 2020
2.152 Impact Factor

LINP1 Promotes the Progression of Cervical Cancer by Scaffolding EZH2, LSD1 and DNMT1 to Inhibit the Expression of KLF2 and PRSS8.

Biochem Cell Biol 2020 Apr 29. Epub 2020 Apr 29.

Guizhou Provincial People's Hospital, 56663, Guiyang, Guizhou, China ;

A growing body of evidence demonstrates that long non-coding RNAs (lncRNAs) are correlated with various cancers. LncRNA LINP1 has been proven as a key factor of malignant tumor development. However, it is still unclear about the role of LINP1 in cervical cancer (CC). Read More

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http://dx.doi.org/10.1139/bcb-2019-0446DOI Listing

Antiviral Effects of Bovine Lactoferrin on Human Norovirus.

Biochem Cell Biol 2020 Apr 29. Epub 2020 Apr 29.

University of Michigan, 1259, Microbiology and Immunology , 1150 West Medical Center Dr, Room 5737MSII , Ann Arbor, Michigan, United States , 48109 ;

Human noroviruses cause significant morbidity and mortality worldwide but lack approved antivirals or vaccines to treat or prevent infections. The recent development of two cell culture systems in human transformed B cells (BJABs) and non-transformed human intestinal enteroids cultures overcomes a main limitation in identifying molecules with anti-norovirus activities. Lactoferrin is an iron-binding glycoprotein found in the milk of most mammals with broad spectrum antimicrobial activities, including against the related murine norovirus in cell culture. Read More

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http://dx.doi.org/10.1139/bcb-2020-0035DOI Listing

Customizable Live-Cell Imaging Chambers for Multimodal and Multiplex Fluorescence Microscopy.

Biochem Cell Biol 2020 Apr 27. Epub 2020 Apr 27.

Western University Schulich School of Medicine and Dentistry, 70384, Microbiology and Immunology , 1151 Richmond Street , Health Sciences Addition, Room H320 , London, Ontario, Canada , N6A 5C1 ;

Using multiple imaging modalities while performing independent experiments in parallel can greatly enhance the throughput of microscopy-based research, but requires provision of appropriate experimental conditions in a format that meets the microscopy's optical requirements. Although customized imaging chambers can meet these challenges, the difficulty of manufacturing custom chambers and the relatively high cost and design inflexibility of commercial chambers has limited the adoption of this approach. Herein, we demonstrate the use of 3D printing to produce inexpensive, customized live-cell imaging chambers that are compatible with a range of imaging modalities including super-resolution microscopy. Read More

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http://dx.doi.org/10.1139/bcb-2020-0064DOI Listing

MicroRNA-518-3p suppresses cell proliferation, invasion and migration in colorectal cancer via targeting TRIP4.

Biochem Cell Biol 2020 Apr 16. Epub 2020 Apr 16.

903 Hospital, Science and Education , No. 9 Huafeng New Village , Jiangyou, Sichuan, China , 621700 ;

MicroRNA (miR)-518-3p has been shown to function as a tumor suppressor. The present study was undertaken to investigate the effects of miR-518-3p in colorectal cancer (CRC). The miR-518-3p mimic, mimic negative control (NC), miR-518-3p inhibitor, inhibitor-NC, ShRNA-TRIP4 and ShRNA-NC vectors were transfected into SW480 cells using Lipofectamine 2000. Read More

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http://dx.doi.org/10.1139/bcb-2019-0442DOI Listing

In Memoriam / In Memoriam.

Biochem Cell Biol 2020 Apr;98(2):iii-iv

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http://dx.doi.org/10.1139/bcb-2020-0048DOI Listing

Lactoferrin and oral pathologies: a therapeutic treatment.

Biochem Cell Biol 2020 Mar 26. Epub 2020 Mar 26.

Universita degli Studi di Roma La Sapienza, 9311, Public Health and Infectious Deseases , P.le Aldo Moro 5 , Roma, Italy , 00185 ;

The oral cavity is a non-uniform, extraordinary environment characterized by mucosal, epithelial, abiotic surfaces and secretions as saliva. Aerobic and anaerobic commensal and pathogenic microorganisms colonize the tongue, teeth, jowl, gingiva and periodontium. Commensals exert an important role in host defenses, while pathogenic microorganisms can nullify this protective function causing oral and systemic diseases. Read More

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http://dx.doi.org/10.1139/bcb-2020-0052DOI Listing

CEMIP regulates the proliferation and migration of vascular smooth muscle cells in atherosclerosis through the WNT-beta-catenin signaling pathway.

Biochem Cell Biol 2020 Apr 24;98(2):249-257. Epub 2020 Mar 24.

Department of Geriatrics, Beijing Jishuitan Hospital, Beijing 100035, China.

In this study we investigated the regulatory role of cell-migration-inducing and hyaluronan-binding protein (CEMIP) in the proliferation and migration of vascular smooth muscle cells (VSMCs). The mRNA and protein levels of CEMIP were upregulated in the plasma samples from patients with atherosclerosis, and in VSMCs stimulated with platelet-derived growth factor-BB (PDGF-BB), compared with plasma from healthy subjects and untreated VSMCs. Silencing CEMIP suppressed PDGF-BB-induced cell migration and proliferation in VSMCs, as determined using a Cell Counting Kit-8 assays, 5-ethynyl-2'-deocyuridine EDU) assays, flow cytometry, wound healing assays, and Transwell assays. Read More

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http://dx.doi.org/10.1139/bcb-2019-0249DOI Listing

PYGB facilitates cell proliferation and invasion in non-small cell lung cancer through activating Wnt/β-catenin signaling.

Biochem Cell Biol 2020 Mar 19. Epub 2020 Mar 19.

Tonglu First People's Hospital, hangzhou , China ;

Brain‑type glycogen phosphorylase (PYGB) has been indicated to be correlated with the progression of various human malignancies. However, its effect and regulatory mechanism in non-small cell lung cancer (NSCLC) are still unclear. Western blotting, immunohistochemistry and qRT-PCR verified that PYGB protein and mRNA expression was up-regulated in both NSCLC cell lines and tissues. Read More

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http://dx.doi.org/10.1139/bcb-2019-0445DOI Listing

Apolipoprotein E2 modulates cell cycle function to promote proliferation in pancreatic cancer cells via regulation of the c-Myc-p21 signalling pathway.

Biochem Cell Biol 2020 Apr 13;98(2):191-202. Epub 2020 Mar 13.

School of Medicine, Nankai University, 94 Weijin Road, Tianjin 300071, China.

Apolipoprotein E2 (ApoE2) is reportedly critical for cell proliferation and survival, and has been identified as a potential tumour-associated marker in many kinds of cancer. However, studies of the function and mechanisms of ApoE2 in pancreatic cancer proliferation and development are rare. In this study, we performed an analysis to determine the modulatory effects of ApoE2-LRP8 (lipoprotein receptor-related protein 8) pathway on cell cycle and cell proliferation, and explored its mechanisms in pancreatic cancer. Read More

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http://dx.doi.org/10.1139/bcb-2018-0230DOI Listing

MicroRNA-760-mediated low expression of DUSP1 impedes the protective effect of NaHS on myocardial ischemia-reperfusion injury.

Biochem Cell Biol 2020 Jun 15;98(3):378-385. Epub 2020 May 15.

Department of Internal Medicine, Hebei Medical University, Shijiazhuang City, Hebei Province 050011, China.

Myocardial ischemia-reperfusion injury (MIRI) is the leading cause of the poor prognosis for patients undergoing clinical cardiac surgery. Micro-RNAs are involved in MIRI; however, the effect of miR-760 on MIRI and the molecular mechanisms behind it have not yet been described. For our in-vivo experiments, 20 rats were randomly distributed between 2 groups ( = 10): the sham-treatment group and the ischemia-reperfusion (I/R) group. Read More

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http://dx.doi.org/10.1139/bcb-2019-0310DOI Listing
June 2020
2.152 Impact Factor

Retraction: Grape seed proanthocyanidins ameliorates cadmium-induced renal injury and oxidative stress in experimental rats through the up-regulation of nuclear related factor 2 and antioxidant responsive elements.

Biochem Cell Biol 2020 04 9;98(2):307. Epub 2020 Mar 9.

Department of Zoology, Faculty of Science, Annamalai University, Annamalainagar 608002, Tamilnadu, India.

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http://dx.doi.org/10.1139/bcb-2020-0070DOI Listing

Down-regulation of NTSR3 inhibits growth, metastasis, and PI3K/AKT and MAPK signaling pathways in colorectal cancer cells.

Biochem Cell Biol 2020 Mar 3. Epub 2020 Mar 3.

First Affiliated Hospital of Jinzhou Medical University, 457651, Jinzhou, Liaoning, China ;

Colorectal cancer is a common malignancy. NTS receptor 3 (NTSR3) plays an important part in multiple cancers. This study aimed to study the effect of NTSR3 on cell growth and metastasis of colorectal cancer. Read More

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http://dx.doi.org/10.1139/bcb-2019-0351DOI Listing

Activity of Fructose-1, 6-bisphosphatase from Campylobacter jejuni.

Biochem Cell Biol 2020 Mar 3. Epub 2020 Mar 3.

University of Leicester, 4488, Leicester, Leicestershire, United Kingdom of Great Britain and Northern Ireland ;

The glycolytic pathway of the enteric pathogen Campylobacter jejuni is incomplete; the absence of phosphofructokinase means that the suppression of futile cycling at this point in the glycolytic/gluconeogenic pathway might not be required and therefore the mechanism of control of pathway flux is likely to be quite different or absent. In this study the characteristics of fructose-1,6-bisphosphatase (FBPase) of C. jejuni are described and the regulation of this enzyme is compared to the equivalent enzymes from organisms capable of glycolysis. Read More

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http://dx.doi.org/10.1139/bcb-2020-0021DOI Listing

LncRNA Gm4419 induces cell apoptosis in hepatic ischemia-reperfusion injury via regulating the miR-455-SOX6 axis.

Biochem Cell Biol 2020 Feb 29:474-483. Epub 2020 Feb 29.

Department of Minimal Invasive Surgery, Ningbo Medical Center Lihuili Hospital, Ningbo City, Zhejiang Province, 315040, China.

Long non-coding RNA (lncRNA) is known to be involved in a variety of diseases. However, the role of Gm4419 in hepatic ischemia-reperfusion (I/R) injury remains unknown. To study this, we first established a rat model of hepatic I/R, and a BRL-3A cell model of hypoxia-reoxygenation (H/R) for in vivo and in vitro studies. Read More

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http://dx.doi.org/10.1139/bcb-2019-0331DOI Listing
February 2020

FILncRNA H19 promotes inflammatory response induced by cerebral ischemia-reperfusion injury through regulating miR-138-5p /p65.

Biochem Cell Biol 2020 Feb 29. Epub 2020 Feb 29.

Hefei Binhu Hospital, Hefei, China ;

Recent study have shown that long non-coding RNA(LncRNA) H19 was up-regulated in brain of rat that suffered from cerebral ischemia-reperfusion (I/R) injury, inducing severe disability and mortality. Molecular mechanisms underlying the involvement of H19 in cerebral I/R injury was unknown. In this study, rat model was induced by transient middle cerebral artery occlusion (tMCAO). Read More

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http://dx.doi.org/10.1139/bcb-2019-0281DOI Listing
February 2020

Notch1-Nrf2 signaling crosstalk provides myocardial protection by reducing ROS formation.

Biochem Cell Biol 2020 Apr 18;98(2):106-111. Epub 2020 Feb 18.

Department of Cardiac Surgery, The First Affiliated Hospital, Nanchang University, Nanchang, China.

Both the Notch1 and Keap1-Nrf2 signaling pathways have cardioprotective effects, but the role of Notch1-Nrf2 crosstalk in myocardial ischemia-reperfusion injury is unclear. In this study, we established hypoxia-reoxygenation in neonate rat myocardial cells and employed γ-secretase inhibitor and curcumin to inhibit and activate the Notch1 and Keap1-Nrf2 signaling pathways, respectively. We found that the combined action of the Notch1 and Keap1-Nrf2 signaling pathways significantly increased cardiomyocyte viability, inhibited cardiomyocyte apoptosis, reduced the formation of reactive oxygen species, and increased antioxidant activities. Read More

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http://dx.doi.org/10.1139/bcb-2018-0398DOI Listing

LncRNA MALAT1 regulates diabetic cardiac fibroblasts through the Hippo/YAP signaling pathway.

Biochem Cell Biol 2020 Feb 18. Epub 2020 Feb 18.

The First Affiliated Hospital of Harbin Medical University, Department of Endocrinology , No. 23 Youzheng Street , Harbin , Heilongjiang, China , 150001 ;

Diabetic cardiomyopathy (DCM) is a major diabetes-related microvascular disease. LncRNA MALAT1 is widely expressed in cardiomyocytes responding to hypoxia and high glucose. In this study, cardiac fibroblasts (CFs) were transfected with si-MALAT1 and exposed to high glucose. Read More

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http://dx.doi.org/10.1139/bcb-2019-0434DOI Listing
February 2020

Investigation of the mechanism of action of a potent pateamine A analog, des-methyl, des-amino pateamine A (DMDAPatA).

Biochem Cell Biol 2020 Feb 2. Epub 2020 Feb 2.

St. John's University, Pharmaceutical Sciences , 8000 Utopia Parkway , St. Albert Hall, 108B , Jamaica, New York, United States , 11439 ;

The natural product pateamineA (PatA) is a highly potent antiproliferative agent. PatA and the simplified analog desmethyl, desamino pateamineA (DMDAPatA) have exhibited cytotoxicity selective for rapidly proliferating cells and have been shown to inhibit cap-dependent translation initiation through binding to eIF4A (eukaryotic initiation factor 4A) of the eIF4F complex. PatA and DMDAPatA are both known to stimulate the RNA-dependent ATPase, and ATP-dependent RNA helicase activities of eIF4A. Read More

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http://dx.doi.org/10.1139/bcb-2019-0307DOI Listing
February 2020

Long noncoding RNA MALAT1 enhances the apoptosis of cardiomyocytes through autophagy modulation.

Biochem Cell Biol 2020 Apr 27;98(2):130-136. Epub 2020 Jan 27.

Department of Cardiovascular, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, 230001, P.R. China.

Induction of autophagy promotes cardiomyocyte survival and confers a cardioprotective effect on acute myocardial infarction (AMI). Our previous study showed that knockdown of long noncoding RNA (lncRNA) metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) attenuated myocardial apoptosis in mouse AMI. Herein, this study further investigated whether the mechanisms by which MALAT1 enhanced cardiomyocyte apoptosis involved the autophagy regulation. Read More

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http://dx.doi.org/10.1139/bcb-2019-0062DOI Listing

Reversal of P-glycoprotein-mediated multidrug resistance by novel curcumin analogues in paclitaxel-resistant human breast cancer cells.

Biochem Cell Biol 2020 Jan 22:1-8. Epub 2020 Jan 22.

Department of Cardiology, Liaocheng People's Hospital Affiliated to Shandong University & Shandong First Medical University, Liaocheng, 252000, China.

Multidrug resistance (MDR) is a major obstacle for successful cancer chemotherapy, and the main cause of MDR has been attributed to overexpression of P-glycoprotein (P-gp). In this present study, four P-gp modulators (,)-4,6-bis(styryl)-2-(substituted amino)-pyrimidines were evaluated for their activity in a breast cancer cell line overexpressing P-gp (LCC6MDR). The four modulators displayed significantly better P-gp modulating activity compared with the positive control verapamil (RF = 5. Read More

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http://dx.doi.org/10.1139/bcb-2019-0377DOI Listing
January 2020
2.152 Impact Factor

Exogenous ubiquitin attenuates hypoxia/reoxygenation-induced cardiac myocyte apoptosis via the involvement of CXCR4 and modulation of mitochondrial homeostasis.

Biochem Cell Biol 2020 Jan 22:1-10. Epub 2020 Jan 22.

Department of Biomedical Sciences, James H Quillen College of Medicine, East Tennessee State University, Johnson City, TN 37614, USA.

Exogenous ubiquitin (UB) plays a protective role in β-adrenergic receptor-stimulated and ischemia/reperfusion (I/R)-induced myocardial remodeling. Here, we report that UB treatment inhibits hypoxia/reoxygenation (H/R)-induced apoptosis in adult rat ventricular myocytes (ARVMs). The activation of Akt was elevated, whereas the activation of glycogen synthase kinase-3β was reduced in UB-treated cells post-H/R. Read More

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http://dx.doi.org/10.1139/bcb-2019-0339DOI Listing
January 2020

Ankyrin-B p.S646F undergoes increased proteasome degradation and reduces cell viability in the H9c2 rat ventricular cardiomyoblast cell line.

Biochem Cell Biol 2020 Apr 22;98(2):299-306. Epub 2020 Jan 22.

Divison of Medical Sciences, University of Victoria, Victoria, BC, Canada.

Ankyrin-B (AnkB) is scaffolding protein that anchors integral membrane proteins to the cardiomyocyte cytoskeleton. We recently identified an AnkB variant, AnkB p.S646F ( c. Read More

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http://dx.doi.org/10.1139/bcb-2019-0082DOI Listing

MiR-1180 promotes cardiomyocyte cell cycle re-entry after injury through the NKIRAS2-NFκB pathway.

Biochem Cell Biol 2020 Jan 19:1-9. Epub 2020 Jan 19.

Department of Emergency, Qingdao Haici Medical Group, Qingdao, China 266034.

Heart failure (HF) is associated with a considerable number of symptoms and significantly impaired health for humans, including reduced quality of life and physical functioning. Previous studies have indicated that miRNAs have important roles in regulating the development of HF. MiR-1180 is involved in the proliferation, migration, invasiveness, and chemoresistance of cancer cells; however, the underlying mechanisms and role of miR-1180 in the functioning of cardiomyocytes remains unclear. Read More

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http://dx.doi.org/10.1139/bcb-2019-0364DOI Listing
January 2020

Protective effect of hydralazine on a cellular model of Parkinson's disease: a possible role of hypoxia-inducible factor (HIF)-1α.

Biochem Cell Biol 2020 Jun 14;98(3):405-414. Epub 2020 May 14.

School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran.

Parkinson's disease (PD) is a neurodegenerative disease accompanied by a low expression level of cerebral hypoxia-inducible factor (HIF-1α). Hence, activating the hypoxia-signaling pathway may be a favorable therapeutic approach for curing PD. This study explored the efficacy of hydralazine, a well-known antihypertensive agent, for restoring the impaired HIF-1 signaling in PD, with the aid of 6-hydroxydopamine (6-OHDA)-exposed SH-SY5Y cells. Read More

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http://dx.doi.org/10.1139/bcb-2019-0117DOI Listing
June 2020
2.152 Impact Factor

Proceedings of the 14th annual RiboWest conference: perspectives and outcome.

Biochem Cell Biol 2020 02 14;98(1):vii-ix. Epub 2020 Jan 14.

Alberta RNA Research and Training Institute (ARRTI), Department of Chemistry & Biochemistry, University of Lethbridge, 4401 University Drive West, T1K 3M4, Lethbridge, Alberta, Canada.

The RiboWest Conference brings together RNA researchers in Canada with the 2-fold goals of fostering internationally competitive RNA research and of training the next generation of scientists. The 14th Annual RiboWest conference (RiboWest 2018) was held at the University of Lethbridge (Lethbridge, Alberta) from June 10th to 13th, 2018. This meeting was focused on all major aspects of RNA research, ranging from understanding the cellular role of RNA, studying RNA interactions and structures, and employing them as a therapeutic tool. Read More

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http://dx.doi.org/10.1139/bcb-2019-0187DOI Listing
February 2020

Inhibition of brain-type glycogen phosphorylase ameliorates high glucose-induced cardiomyocyte apoptosis via Akt-HIF-1α activation.

Biochem Cell Biol 2020 Jan 6:458-465. Epub 2020 Jan 6.

The National Engineering Research Center for Bioengineering Drugs and the Technologies, Institute of Translational Medicine, Nanchang University, 1299 Xuefu Avenue, Nanchang 330031, P.R. China.

Brain-type glycogen phosphorylase (pygb) is one of the rate-limiting enzymes in glycogenolysis that plays a crucial role in the pathogenesis of type 2 diabetes mellitus. Here we investigated the role of pygb in high-glucose (HG)-induced cardiomyocyte apoptosis and explored the underlying mechanisms, by using the specific pygb inhibitors or pygb siRNA. Our results show that inhibition of pygb significantly attenuates cell apoptosis and oxidative stress induced by HG in H9c2 cardiomyocytes. Read More

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http://dx.doi.org/10.1139/bcb-2019-0247DOI Listing
January 2020

miR-140-5p targeted FGF9 and inhibited the cell growth of laryngeal squamous cell carcinoma.

Biochem Cell Biol 2020 Apr 23;98(2):83-89. Epub 2019 Dec 23.

Department of Otolaryngology Head and Neck, Guangdong General Hospital and Guangdong Academy of Medical Sciences, Guangzhou, 510080 Guangdong, P.R. China.

Increasing evidence has suggested that microRNAs (miRNAs) play critical roles in the initiation and development of cancers. Here, we found that miR-140-5p was significantly downregulated in both laryngeal squamous cell carcinoma (LSCC) tissues and cell lines. Decreased expression of miR-140-5p was significantly associated with the metastasis of LSCC. Read More

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http://dx.doi.org/10.1139/bcb-2018-0351DOI Listing

Active vitamin D activates chondrocyte autophagy to reduce osteoarthritis via mediating the AMPK-mTOR signaling pathway.

Biochem Cell Biol 2020 Jun 9;98(3):434-442. Epub 2019 Dec 9.

Department of Rheumatic Immunology, Tianjin First Central Hospital, Tianjin 300190, P.R. China.

Osteoarthritis (OA) is a common joint degenerative disease. Vitamin D (VD) is essential for bone health. We hypothesized that active VD could be used as a therapeutic treatment for OA. Read More

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http://dx.doi.org/10.1139/bcb-2019-0333DOI Listing

Glycerol kinase enhances hepatic lipid metabolism by repressing nuclear receptor subfamily 4 group A1 in the nucleus.

Biochem Cell Biol 2020 Jun 7;98(3):370-377. Epub 2019 Dec 7.

Department of Experimental Hematology and Biochemistry, Beijing Institute of Radiation Medicine, Beijing 100850, China.

Glycerol kinase (GYK) plays a critical role in hepatic metabolism by converting glycerol to glycerol 3-phosphate in an ATP-dependent reaction. GYK isoform b is the only glycerol kinase present in whole cells, and has a non-enzymatic moonlighting function in the nucleus. GYK isoform b acts as a co-regulator of nuclear receptor subfamily 4 group A1 (NR4A1) and participates in the regulation of hepatic glucose metabolism by protein-protein interaction with NR4A1. Read More

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http://dx.doi.org/10.1139/bcb-2019-0317DOI Listing

Circ_KATNAL1 regulates prostate cancer cell growth and invasiveness through the miR-145-3p/WISP1 pathway.

Biochem Cell Biol 2020 Jun 4;98(3):396-404. Epub 2019 Dec 4.

Department of Urology, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou 510260, China.

Prostate cancer (PCa) is the second leading cause of death in men, and current studies have shown that circular RNAs (circRNAs) play important roles in its occurrence and development. Detection of circRNAs in PCa cells showed that circ_KATNAL1 is down-regulated, mainly located in the cytoplasm, and contains multiple binding sites of miR-145-3p, which is an anticancer miRNA. RNA immunoprecipitation with anti-AGO2 antibody, RNA pull-down assays with biotin-labeled circ_KATNAL1 probe or an miR-145-3p mimic, and dual luciferase reporter gene assays confirmed that circ_KATNAL1 binds directly to miR-145-3p in cells, and that , which is highly expressed in many types of tumors, is an important target gene of miR-145-3p. Read More

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http://dx.doi.org/10.1139/bcb-2019-0211DOI Listing

Homogeneity and heterogeneity of biological characteristics in mesenchymal stem cells from human umbilical cords and exfoliated deciduous teeth.

Biochem Cell Biol 2020 Jun 3;98(3):415-425. Epub 2019 Dec 3.

Stem Cells and Regenerative Medicine Research Center, Sichuan Stem Cell Bank/Sichuan Neo-life Stem Cell Biotech Inc., Chengdu, China.

Mesenchymal stem cells (MSCs) have proven powerful potential for cell-based therapy both in regenerative medicine and disease treatment. Human umbilical cords and exfoliated deciduous teeth are the main sources of MSCs with no donor injury or ethical issues. The goal of this study was to investigate the differences in the biological characteristics of human umbilical cord mesenchymal stem cells (UCMSCs) and stem cells from human exfoliated deciduous teeth (SHEDs). Read More

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http://dx.doi.org/10.1139/bcb-2019-0253DOI Listing
June 2020
2.152 Impact Factor

Omega-3 polyunsaturated fatty acids have beneficial effects on visceral fat in diet-induced obesity model.

Biochem Cell Biol 2019 12 27;97(6):693-701. Epub 2019 Nov 27.

Laboratório de Neurobiologia de Processos Inflamatórios e Metabólicos, Programa de Pós-Graduação em Ciências da Saúde, Universidade do Sul de Santa Catarina, Tubarão, Santa Catarina, Brasil.

This study evaluated the effects of omega-3 polyunsaturated fatty acids (PUFAs) on oxidative stress and energy metabolism parameters in the visceral fat of a high-fat-diet induced obesity model. Energy intake, body mass, and visceral fat mass were also evaluated. Male Swiss mice received either a control diet (control group) or a high-fat diet (obese group) for 6 weeks. Read More

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http://dx.doi.org/10.1139/bcb-2018-0361DOI Listing
December 2019
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