622 results match your criteria Biochemistry and biophysics reports[Journal]


Establishment of a monoclonal antibody PMab-225 against alpaca podoplanin for immunohistochemical analyses.

Biochem Biophys Rep 2019 Jul 5;18:100633. Epub 2019 Apr 5.

Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi, 980-8575, Japan.

Podoplanin (PDPN) is known as a lymphatic endothelial cell marker. Monoclonal antibodies (mAbs) against human, mouse, rat, rabbit, dog, cat, bovine, pig, and horse PDPN have been established in our previous studies. However, mAbs against alpaca PDPN (aPDPN), required for immunohistochemical analysis, remain to be developed. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbrep.2019.100633DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6451175PMC

Tandem repeats of the 5' flanking region of human have a role as a novel enhancer in gene expression.

Biochem Biophys Rep 2019 Jul 4;18:100632. Epub 2019 Apr 4.

Department of Gastroenterology, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8655, Japan.

is a well-known gastric differentiation marker, which has been frequently used for the classification of stomach cancer. However, the molecular mechanism of regulation of expression remains to be elucidated. In previous studies, we have shown that Gli regulated transcription through the Gli-binding motif in the 5' region of . Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbrep.2019.100632DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6449733PMC
July 2019
1 Read

Spectroscopic and viscometric determination of DNA-binding modes of some bioactive dibenzodioxins and phenazines.

Biochem Biophys Rep 2019 Jul 4;18:100629. Epub 2019 Apr 4.

Department of Biological Sciences, BITS Pilani KK Birla Goa Campus, Zuarinagar, Goa, 403726, India.

Push-pull dibenzodioxins and phenazines having 'anthracene-like' planar structures and good charge transfer character had been previously synthesised in our laboratory. The dibenzodioxins had earlier proven their anti-proliferative nature against HeLa tumor cell lines. Since phenazines are structural analogues of the former, these molecules were evaluated in course of the current study for their cytotoxic action against HeLa cell lines and they exhibited strong anti-tumor activity. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbrep.2019.100629DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6449707PMC

Establishment of a monoclonal antibody PMab-233 for immunohistochemical analysis against Tasmanian devil podoplanin.

Biochem Biophys Rep 2019 Jul 1;18:100631. Epub 2019 Apr 1.

Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi, 980-8575, Japan.

Monoclonal antibodies (mAbs) against not only human, mouse, and rat but also rabbit, dog, cat, bovine, pig, and horse podoplanins (PDPNs) have been established in our previous studies. PDPN is used as a lymphatic endothelial cell marker in pathological diagnoses. However, mAbs against Tasmanian devil PDPN (tasPDPN), which are useful for immunohistochemical analysis, remain to be developed. Read More

View Article

Download full-text PDF

Source
https://linkinghub.elsevier.com/retrieve/pii/S24055808193004
Publisher Site
http://dx.doi.org/10.1016/j.bbrep.2019.100631DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6446048PMC
July 2019
1 Read

Von Willebrand Factor Type A domain of hCLCA1 is sufficient for U-937 macrophage activation.

Biochem Biophys Rep 2019 Jul 28;18:100630. Epub 2019 Mar 28.

Department of Veterinary Biomedical Sciences, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.

The human hCLCA1 gene is a member of the gene family that has a well-documented role in inflammatory airway diseases. Previously, we demonstrated that secreted hCLCA1 plays a role in regulating the innate immune response by activating airway macrophages. However, the mechanism of this regulation remains unclear. Read More

View Article

Download full-text PDF

Source
https://linkinghub.elsevier.com/retrieve/pii/S24055808183021
Publisher Site
http://dx.doi.org/10.1016/j.bbrep.2019.100630DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6444176PMC
July 2019
1 Read

Phosphorylated-Akt overexpression is associated with a higher risk of brain metastasis in patients with non-small cell lung cancer.

Biochem Biophys Rep 2019 Jul 25;18:100625. Epub 2019 Mar 25.

Department of Oncology, Tongji Hospital, Huazhong University of Science and Technology, Wuhan, Hubei Province, China.

Brain metastasis (BM) of non-small cell lung cancer (NSCLC) is relatively common and has a poor prognosis. Moreover, identifying which patients are more likely to develop BM is challenging. Akt, a serine/threonine-specific protein kinase, can be activated in various tumors, including lung cancer, and may be associated with poor prognosis. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbrep.2019.100625DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6444023PMC
July 2019
1 Read

Potassium chloride released from contracting skeletal muscle may stimulate development of its hypertrophy.

Biochem Biophys Rep 2019 Jul 21;18:100627. Epub 2019 Mar 21.

Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Ave. 33, Bld. 2, Moscow, 119071, Russia.

The effects of potassium chloride on the expression of IGF-1 splice forms and myoblast proliferation were investigated. KCl at the concentrations of 7-12 mM stimulated the synthesis of IGF-1 and mechano growth factor (MGF) in murine myoblasts as well as in myotubes both at the mRNA and protein levels. Pan-calcium channel blocker CdCl completely abolished stimulation of growth factor expression, whereas blocker of HCN and Na1. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbrep.2019.100627DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6433999PMC

Contributions of the C-terminal domain to poly(A)-specific ribonuclease (PARN) stability and self-association.

Biochem Biophys Rep 2019 Jul 19;18:100626. Epub 2019 Mar 19.

State Key Laboratory of Membrane Biology, School of Life Sciences, Tsinghua University, Beijing, 100084, China.

Poly(A)-specific ribonuclease (PARN) catalyzes the degradation of mRNA poly(A) tail to regulate translation efficiency and mRNA decay in higher eukaryotic cells. The full-length PARN is a multi-domain protein containing the catalytic nuclease domain, the R3H domain, the RRM domain and the C-terminal intrinsically unstructured domain (CTD). The roles of the three well-structured RNA-binding domains have been extensively studied, while little is known about CTD. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbrep.2019.100626DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6430076PMC

Delta-6-desaturase (FADS2) inhibition and omega-3 fatty acids in skeletal muscle protein turnover.

Biochem Biophys Rep 2019 Jul 15;18:100622. Epub 2019 Mar 15.

University of Memphis, School of Health Studies, Memphis, TN, USA.

Polyunsaturated fatty acids (PUFAs) are essential dietary components. They are not only used for energy, but also act as signaling molecules. The delta-6 desaturase (D6D) enzyme, encoded by the FADS2 gene, is one of two rate limiting enzymes that convert the PUFA precursors - α-linolenic (n-3) and linoleic acid (n-6) to their respective metabolites. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbrep.2019.100622DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6424014PMC

A cell-based high-throughput screen identifies tyrphostin AG 879 as an inhibitor of animal cell phospholipid and fatty acid biosynthesis.

Biochem Biophys Rep 2019 Jul 6;18:100621. Epub 2019 Mar 6.

Department of Physiology & Biophysics, Boston University School of Medicine, 700 Albany Street, Room W302, Boston, MA, 02118, USA.

Inhibition of animal cell phospholipid biosynthesis has been proposed for anticancer and antiviral therapies. Using CHO-K1 derived cell lines, we have developed and used a cell-based high-throughput procedure to screen a 1280 compound, small molecule library for inhibitors of phospholipid biosynthesis. We identified tyrphostin AG 879 (AG879), which inhibited phospholipid biosynthesis by 85-90% at a concentration of 10 μM, displaying an IC of 1-3 μM. Read More

View Article

Download full-text PDF

Source
https://linkinghub.elsevier.com/retrieve/pii/S24055808193000
Publisher Site
http://dx.doi.org/10.1016/j.bbrep.2019.100621DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6406593PMC
July 2019
2 Reads

Neuronal NO synthase mediates plenylephrine induced cardiomyocyte hypertrophy through facilitation of NFAT-dependent transcriptional activity.

Biochem Biophys Rep 2019 Jul 11;18:100620. Epub 2019 Mar 11.

INSERM U1048 -Institut des Maladies Métaboliques et Cardiovacsulaires (I2MC), Toulouse, France.

Neuronal nitric oxide synthase (NOS1) has been consistently shown to be the predominant isoform of NOS and/or NOS-derived NO that may be involved in the myocardial remodeling including cardiac hypertrophy. However, the direct functional contribution of NOS1 in this process remains to be elucidated. Therefore, in the present study, we attempted to use silent RNA and adenovirus mediated silencing or overexpression to investigate the role of NOS1 and the associated molecular signaling mechanisms during OKphenylephrine (PE)-induced cardiac hypertrophy growth in neonatal rat ventricular cardiomyocytes (NRVMs). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbrep.2019.100620DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6412025PMC

Convenient methodology for extraction and subsequent selective propagation of mouse melanocytes in culture from adult mouse skin tissue.

Biochem Biophys Rep 2019 Jul 22;18:100619. Epub 2019 Feb 22.

Department of Cell Biology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.

Mouse melanoma B16-BL6 cells are useful cells for cancer metastatic studies. To understand the metastatic principle at molecular levels, it is necessary to carry out experiments in which cancer cells and their normal counterparts are compared. However, unlike normal human melanocytes, preparation of normal mouse melanocytes is quite difficult due to the lack of marketing and insufficient information on an established protocol for primary culture of mouse melanocytes. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbrep.2019.100619DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6406582PMC

15-deoxy-Δ-prostaglandin J enhances anticancer activities independently of VHL status in renal cell carcinomas.

Biochem Biophys Rep 2019 Jul 14;18:100608. Epub 2019 Feb 14.

Department of Pharmaceutical Health Care, Faculty of Pharmaceutical Sciences, Himeji Dokkyo University, 2-1, kami-ohno 7-Chome, Himeji, Hyogo 670-8524, Japan.

Renal cell carcinoma (RCC) is relatively resistant to chemotherapy and radiotherapy. Clear cell RCC (ccRCC) accounts for the majority of RCC, which have mutations or epigenetic silencing of the () gene. VHL-positive Caki-2 cells are killed by an endogenous anticancer substance, 15-deoxy-Δ-prostaglandin J (15d-PGJ). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbrep.2019.01.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6377412PMC
July 2019
1 Read

Quercetin glycosides prevent dexamethasone-induced muscle atrophy in mice.

Biochem Biophys Rep 2019 Jul 11;18:100618. Epub 2019 Feb 11.

Institute for Health Care Science, Suntory Wellness Ltd, 8-1-1 Seikadai, Seika-cho, Soraku-gun, Kyoto, 619-0284, Japan.

Although quercetin has numerous biological benefits, including preventing muscle atrophy due to disuse, no reports have been published to date about the preventive effects and molecular mechanisms underlying drug-induced muscle atrophy. Highly soluble and bioavailable quercetin glycosides (QGs) were used to examine the inhibition of dexamethasone (DEX)-induced muscle atrophy in vivo. Male BALB/cCrSlc mice were treated with or without QGs for 7 days ad libitum, followed by addition of DEX to their drinking water for a further 7 days. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbrep.2019.100618DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6372881PMC

Rapamycin suppresses postnatal muscle hypertrophy induced by myostatin-inhibition accompanied by transcriptional suppression of the Akt/mTOR pathway.

Biochem Biophys Rep 2019 Mar 21;17:182-190. Epub 2019 Jan 21.

Department of Human Nutrition, Food and Animal Sciences, University of Hawaii, Honolulu, HI 96822, USA.

Myostatin (MSTN) is a well-known negative growth factor of muscle mass, and studies have shown that MSTN-inhibition would be a potential strategy to treat muscle atrophy seen in various clinical conditions. Recent studies suggest that MSTN-inhibition induces skeletal muscle hypertrophy through up-regulation of the anabolic Akt/mTOR pathway. Therefore, it was hypothesized that the muscle hypertrophy induced by MSTN-inhibition would be suppressed by the administration of rapamycin (RAP), a mTOR suppressor. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbrep.2018.12.009DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6362869PMC

Streptavidin cooperative allosterism upon binding biotin observed by differential changes in intrinsic fluorescence.

Biochem Biophys Rep 2019 Mar 3;17:127-131. Epub 2019 Jan 3.

Department of Chemistry, John Carroll University, 1 John Carroll Blvd., University Heights, OH 44118, USA.

While the binding of biotin by streptavidin does not appear to be cooperative in the traditional sense of altered binding strength, it has been suggested that it may be cooperative in terms of differential structural changes in the protein. In this work we present intrinsic tryptophan fluorescence data as evidence of a cooperative structural change. The technique involves examination of the differences in fluorescence emission corresponding to distinct tryptophan populations accompanying protein-ligand binding. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbrep.2018.12.011DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6372923PMC

NRLMF: Beta-distribution-rescored neighborhood regularized logistic matrix factorization for improving the performance of drug-target interaction prediction.

Biochem Biophys Rep 2019 Jul 7;18:100615. Epub 2019 Feb 7.

School of Computing, Tokyo Institute of Technology, 2-12-1 W8-76 Ookayama, Meguro-ku, Tokyo, 152-8550, Japan.

Techniques for predicting interactions between a drug and a target (protein) are useful for strategic drug repositioning. Neighborhood regularized logistic matrix factorization (NRLMF) is one of the state-of-the-art drug-target interaction prediction methods; it is based on a statistical model using the Bernoulli distribution. However, the prediction is not accurate when drug-target interaction pairs have less interaction information (e. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbrep.2019.01.008DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6370585PMC

Mass spectrometry analysis of the human endosulfatase Hsulf-2.

Biochem Biophys Rep 2019 Jul 7;18:100617. Epub 2019 Feb 7.

Université Paris-Saclay, CNRS, CEA, Univ Evry, LAMBE, 91025, Evry, France.

The human 6--endosulfatases HSulf-1 and -2 catalyze the region-selective hydrolysis of the 6--sulfate group of the glucosamine residues within sulfated domains of heparan sulfate, thereby ensuring a unique and original post-biosynthetic modification of the cell surface proteoglycans. While numerous studies point out the role of HSulf-2 in crucial physiological processes as well as in pathological conditions particularly in cancer, its structural organization in two chains and its functional properties remain poorly understood. In this study, we report the first characterization by mass spectrometry (MS) of HSulf-2. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbrep.2019.01.010DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6369371PMC

PMab-219: A monoclonal antibody for the immunohistochemical analysis of horse podoplanin.

Biochem Biophys Rep 2019 Jul 2;18:100616. Epub 2019 Feb 2.

Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi, 980-8575, Japan.

Monoclonal antibodies (mAbs) against human, mouse, rat, rabbit, dog, cat, and bovine podoplanin (PDPN), a lymphatic endothelial cell marker, have been established in our previous studies. However, mAbs against horse PDPN (horPDPN), which are useful for immunohistochemical analysis, remain to be developed. In the present study, mice were immunized with horPDPN-overexpressing Chinese hamster ovary (CHO)-K1 cells (CHO/horPDPN), and hybridomas producing mAbs against horPDPN were screened using flow cytometry. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbrep.2019.01.009DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6360987PMC
July 2019
1 Read

Phyto-assisted synthesis of zinc oxide nanoparticles using and its antibacterial activity against .

Biochem Biophys Rep 2019 Mar 28;17:208-211. Epub 2019 Jan 28.

Department of Prosthodontics, Saveetha Dental College and Hospitals, Saveetha University, SIMATS, Chennai 600077, TN, India.

Zinc oxide, an established inorganic metal oxide in nanoparticles form exhibits tremendous anti-bacterial activity. The present study focuses on determining the anti-bacterial activity of green synthesized zinc oxide nanoparticles (ZnO NPs). Results clearly validate the effective synthesis of spherical shaped nanoparticles with average size range of 60-80 nm. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbrep.2019.01.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6351291PMC
March 2019
7 Reads

Mannose-decorated hybrid nanoparticles for enhanced macrophage targeting.

Biochem Biophys Rep 2019 Mar 25;17:197-207. Epub 2019 Jan 25.

Department of Pharmaceutical Sciences, College of Pharmacy, University of Tennessee Health Science Center, Memphis, TN 38163, USA.

Our goal was to design nanocarriers that specifically target and deliver therapeutics to polarized macrophages. Mannose receptors are highly overexpressed on polarized macrophages. In this study, we constructed Pluronic® -F127 polymer and tannic acid (TA) based nanoparticles (F127-TA core nanoparticles) with varying mannose densities. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbrep.2019.01.007DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6351286PMC
March 2019
1 Read

Use of body composition measurements to guide the assessment of dry weight in anuric dialysis patients: improvements in blood pressure control.

Biochem Biophys Rep 2019 Mar 24;17:191-196. Epub 2019 Jan 24.

BaoTou Central Hospital Blood Purification Center, BaoTou 014040, China.

Purpose: Fluid management using a body composition monitor (BCM) based on bioimpedance spectroscopy has been found to be beneficial for maintenance hemodialysis (MHD) patients. Our purpose was to provide a management procedure for the adjustment of post-dialysis overhydration (OH) and to evaluate whether this approach could improve blood pressure.

Methods: Post-dialysis fluid status was assessed weekly using the BCM. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbrep.2019.01.005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6351389PMC

Selective androgen receptor modulator, S42 has anabolic and anti-catabolic effects on cultured myotubes.

Biochem Biophys Rep 2019 Mar 15;17:177-181. Epub 2019 Jan 15.

Department of Endocrinology and Diabetes Mellitus, Faculty of Medicine, Fukuoka University, 7-45-1 Nanakuma, Jonan-ku, Fukuoka 814-0180, Japan.

We previously identified a novel selective androgen receptor modulator, S42, that does not stimulate prostate growth but has a beneficial effect on lipid metabolism. S42 also increased muscle weight of the levator ani in orchiectomized Sprague-Dawley rats. These findings prompted us to investigate whether S42 has a direct effect on cultured C2C12 myotubes. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbrep.2019.01.006DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6348734PMC
March 2019
4 Reads

Recombinant asialoerythropoetin protects HL-1 cardiomyocytes from injury via suppression of Mst1 activation.

Biochem Biophys Rep 2019 Mar 9;17:157-168. Epub 2019 Jan 9.

Department of Pharmaceutical Sciences, Biomanufacturing Research Institute & Technology Enterprise, North Carolina Central University, Durham, NC 27707, USA.

Background: Recombinant human erythropoietin (rhuEPO) and asialoerythropoietin (asialo-rhuEPO) are cardioprotective. However, the protective effects of rhuEPO could not be translated into clinical practice because of its hematopoiesis-associated side effects while non-erythropoietic asialo-rhuEPO is unavailable in large quantities for clinical studies. This study was designed to investigate the cardiomyocyte protective potential of plant-produced asialo-rhuEPO (asialo-rhuEPO) against staurosporine (STS)-induced injury in HL-1 murine cardiomyocytes and identify cellular pathway(s) responsible for its cardioprotection. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbrep.2019.01.004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6327940PMC

The regulatory effect of Tau protein on polymerization of MCF7 microtubules .

Biochem Biophys Rep 2019 Mar 9;17:151-156. Epub 2019 Jan 9.

Department of Physics, Seton Hall University, 400 South Orange Ave., South Orange, NJ 07079, USA.

Growing evidence continues to point toward the critical role of beta tubulin isotypes in regulating some intracellular functions. Changes that were observed in the microtubules' intrinsic dynamics, the way they interact with some chemotherapeutic agents, or differences on translocation specifications of some molecular motors along microtubules, were associated to their structural uniqueness in terms of beta tubulin isotype distributions. These findings suggest that the effects of microtubule associated proteins (MAPs) may also vary on structurally different microtubules. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbrep.2018.12.010DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6327910PMC

TRPC6 inactivation does not affect loss of renal function in nephrotoxic serum glomerulonephritis in rats, but reduces severity of glomerular lesions.

Biochem Biophys Rep 2019 Mar 8;17:139-150. Epub 2019 Jan 8.

Department of Biology and Biochemistry, University of Houston, Houston, TX, USA.

Canonical transient receptor potential-6 (TRPC6) channels have been implicated in a variety of chronic kidney diseases including familial and acquired forms of focal and segmental glomerulosclerosis (FSGS) and renal fibrosis following ureteral obstruction. Here we have examined the role of TRPC6 in progression of inflammation and fibrosis in the nephrotoxic serum (NTS) model of crescentic glomerulonephritis. This was assessed in rats with non-functional TRPC6 channels due to genomic disruption of an essential domain in TRPC6 channels ( rats) and wild-type littermates ( rats). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbrep.2018.12.006DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6325086PMC
March 2019
2 Reads

Involvement of the NADPH oxidase 2 pathway in renal oxidative stress in mice.

Biochem Biophys Rep 2019 Mar 11;17:169-176. Epub 2019 Jan 11.

Department of Veterinary Pharmacology, University of Miyazaki, Miyazaki 889-2192, Japan.

Aquaporin-11 (AQP11) is an intracellular AQP. Several studies with mice have shown that AQP11 has a role in normal development of the kidney after birth. Our previous studies have suggested that alteration of oxygen homeostasis may be involved in the kidney injury caused by AQP11 deficiency, although the underlying mechanism is largely unknown. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbrep.2019.01.003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6329705PMC
March 2019
1 Read

Konjac ceramide (kCer) regulates keratinocyte migration by Sema3A-like repulsion mechanism.

Biochem Biophys Rep 2019 Mar 3;17:132-138. Epub 2019 Jan 3.

Lipid Biofunction Section, Frontier Research Center for Advanced Material and Life Science, Faculty of Advanced Life Science, Hokkaido University, Sapporo, Hokkaido, Japan.

Previously, we proposed the following mechanism for konjac ceramide (kCer)-mediated neurite outgrowth inhibition: kCer binds to Nrp as a Sema3A agonist, resulting in Nrp1/PlexA complex formation and activation of the Sema3A signaling pathway to induce phosphorylation of CRMP2 and microtubule depolymerization. The Sema3A/Nrp1 signaling pathway is known to be also expressed in normal human keratinocytes. To determine whether kCer can function in human keratinocytes as it does in neurites, that is, if it can bind to Nrp1 in place of Sema3A, we studied the effect of kCer on HaCaT cell migration activity. Read More

View Article

Download full-text PDF

Source
https://linkinghub.elsevier.com/retrieve/pii/S24055808183014
Publisher Site
http://dx.doi.org/10.1016/j.bbrep.2018.11.004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6319021PMC
March 2019
8 Reads

Neuroprotective efficacy of thymoquinone against amyloid beta-induced neurotoxicity in human induced pluripotent stem cell-derived cholinergic neurons.

Biochem Biophys Rep 2019 Mar 3;17:122-126. Epub 2019 Jan 3.

Department of Electronics, Graduate School of Engineering, Tohoku Institute of Technology, 35-1 Yagiyama Kasumicho, Taihaku-ku, Sendai, Miyagi 192-0982, Japan.

The natural antioxidant Thymoquinone (TQ) is the most abundant ingredient in the curative plant Nigella sativa seed's oil. An extensive number of studies have revealed that TQ is the most active and most responsible component for the plant's pharmacological properties. It has been documented in several studies that TQ has a wide range of protective activities and many neuropharmacological attributes. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbrep.2018.12.005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6317145PMC
March 2019
1 Read

Intravascular heavy chain-modification of hyaluronan during endotoxic shock.

Biochem Biophys Rep 2019 Mar 26;17:114-121. Epub 2018 Dec 26.

Department of Medicine, National Jewish Health, 1400 Jackson Street, Denver, CO 80206, USA.

During inflammation, the covalent linking of the ubiquitous extracellular polysaccharide hyaluronan (HA) with the heavy chains (HC) of the serum protein inter alpha inhibitor (IαI) is exclusively mediated by the enzyme tumor necrosis factor α (TNFα)-stimulated-gene-6 (TSG-6). While significant advances have been made regarding how HC-modified HA (HC-HA) is an important regulator of inflammation, it remains unclear why HC-HA plays a critical role in promoting survival in intraperitoneal lipopolysaccharide (LPS)-induced endotoxemia while exerting only a modest role in the outcomes following intratracheal exposure to LPS. To address this gap, the two models of intraperitoneal LPS-induced endotoxic shock and intratracheal LPS-induced acute lung injury were directly compared in knockout mice and littermate controls. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbrep.2018.12.007DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6307094PMC
March 2019
2 Reads

Hyperinsulinemic hypoglycemia subtype glucokinase V91L mutant induces necrosis in β-cells via ATP depletion.

Biochem Biophys Rep 2019 Mar 20;17:108-113. Epub 2018 Dec 20.

Department of Molecular Medicine, Mayo Clinic, Rochester, MN, USA.

Hyperinsulinemic hypoglycemia subtype glucokinase (GCK-HH) is caused by an activating mutation in glucokinase (GCK) and has been shown to increase β-cell death. However, the mechanism of β-cell death in GCK-HH remains poorly understood. Here, we expressed the GCK-HH V91L GCK mutant in INS-1 832/13 cells to determine the effect of the mutation on β-cell viability and the mechanisms of β-cell death. Read More

View Article

Download full-text PDF

Source
https://linkinghub.elsevier.com/retrieve/pii/S24055808183019
Publisher Site
http://dx.doi.org/10.1016/j.bbrep.2018.12.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6304456PMC
March 2019
6 Reads

Metabolic treatment of syndrome linked with Parkinson's disease and hypothalamus pituitary gonadal hormones by turmeric curcumin in Bisphenol-A induced neuro-testicular dysfunction of wistar rat.

Biochem Biophys Rep 2019 Mar 13;17:97-107. Epub 2018 Dec 13.

Toxicology and Safety Unit, Department of Environmental Health Sciences, Faculty of Public Health, College of Medicine, University of Ibadan, Nigeria.

The metabolic shift in cholinesterase activity and inhibitor of hypothalamus pituitary gonadal hormones were hypothesized as resultant effect of Parkinson's disease (PD) which is clinically characterized by a movement disorder. This study therefore examined the effect of turmeric curcumin (CUR) on index of PD, acetylcholine esterase activity and disorder of hypothalamus pituitary gonadal hormone (HPGH) in Bisphenol-A induced injury using animal model. Forty adult male albino rats were randomly distributed into five (n = 8) groups. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbrep.2018.12.004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6296165PMC
March 2019
1 Read

Variants of the industrially relevant protease KP-43 with suppressed activity under alkaline conditions developed using expanded genetic codes.

Biochem Biophys Rep 2019 Mar 12;17:93-96. Epub 2018 Dec 12.

Biological Science Research, Kao Corporation, 1334 Minato, Wakayama, Wakayama 640-8580, Japan.

In the present study, we attempted to control the pH profile of the catalytic activity of the industrially relevant alkaline protease KP-43, by incorporating 3-nitro-l-tyrosine and 3-chloro-l-tyrosine at and near the catalytic site. Thirty KP-43 variants containing these non-natural amino acids at the specific positions were synthesized in host cells with expanded genetic codes. The variant with 3-nitrotyrosine at position 205, near the substrate binding site, retained its catalytic activity at the neutral pH and showed a 60% activity reduction at pH 10. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbrep.2018.12.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6295607PMC

Hepatocyte nuclear factor 4α regulates megalin expression in proximal tubular cells.

Biochem Biophys Rep 2019 Mar 12;17:87-92. Epub 2018 Dec 12.

Division of Molecular Science, Graduate School of Science and Technology, Gunma University, Kiryu, Gunma 376-8515, Japan.

Hepatocyte nuclear factor 4α (HNF4α) is a member of the nuclear receptor superfamily and upregulates expression of many genes in the liver, pancreas, small intestine, and colon. HNF4α is also highly expressed in proximal tubular epithelial cells (PTECs) in kidney. PTECs reabsorb various substances through transporters, ion channels, and receptors, but the target genes for HNF4α in PTECs have not been investigated in detail. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbrep.2018.11.010DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6295598PMC

Establishment of X-linked Alport syndrome model mice with a R471X mutation.

Biochem Biophys Rep 2019 Mar 12;17:81-86. Epub 2018 Dec 12.

Pharmaceutical Research Division, Takeda Pharmaceutical Company Limited, 2-26-1, Muraoka-Higashi, Fujisawa, Kanagawa, 251-8555, Japan.

Alport syndrome (AS) is an inherited disorder characterized by glomerular basement membrane (GBM) abnormality and development of chronic kidney disease at an early age. The cause of AS is a genetic mutation in type IV collagen, and more than 80% of patients have X-linked AS (XLAS) with mutation in . Although the causal gene has been identified, mechanisms of progression have not been elucidated, and no effective treatment has been developed. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbrep.2018.12.003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6295608PMC
March 2019
3 Reads

Synthesis and characterization of zinc oxide nanoparticles by using polyol chemistry for their antimicrobial and antibiofilm activity.

Biochem Biophys Rep 2019 Mar 12;17:71-80. Epub 2018 Dec 12.

Centre for Interdisciplinary Research, D.Y. Patil University, Kolhapur, India.

The present investigation deals with facile polyol mediated synthesis and characterization of ZnO nanoparticles and their antimicrobial activities against pathogenic microorganisms. The synthesis process was carried out by refluxing zinc acetate precursor in diethylene glycol(DEG) and triethylene glycol(TEG) in the presence and in the absence of sodium acetate for 2 h and 3 h. All synthesized ZnO nanoparticles were characterized by X-ray diffraction (XRD), UV visible spectroscopy (UV), thermogravimetric analysis (TGA), fourier transform infrared spectroscopy (FTIR), field emission scanning electron microscopy(FESEM), transmission electron microscopy (TEM) and energy dispersive X-ray spectroscopy (EDX) technique. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbrep.2018.11.007DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6295600PMC
March 2019
4 Reads

The role of cytochrome P450 2E1 on ethanol-mediated oxidative stress and HIV replication in human monocyte-derived macrophages.

Biochem Biophys Rep 2019 Mar 6;17:65-70. Epub 2018 Dec 6.

Department of Pharmaceutical Sciences, College of Pharmacy, University of Tennessee Health Science Center, Memphis, TN 38163, USA.

Background: Alcohol consumption is considered to be a major health problem among people living with HIV/AIDS. Our previous reports have shown that ethanol reduced intracellular concentrations of antiretroviral drugs elvitegravir and darunavir in the HIV-1-infected U1 cell line. Ethanol also increased HIV-1 replication despite the presence of elvitegravir. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbrep.2018.11.008DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6295597PMC
March 2019
8 Reads

RasGRF1 mediates brain-derived neurotrophic factor-induced axonal growth in primary cultured cortical neurons.

Biochem Biophys Rep 2019 Mar 6;17:56-64. Epub 2018 Dec 6.

Laboratory of Molecular Neurobiology, Graduate School of Pharmaceutical Sciences, Kyoto University, Yoshidakonoe-cho, Sakyo-ku, Kyoto 606-8501, Japan.

The appropriate development and regulation of neuronal morphology are important to establish functional neuronal circuits and enable higher brain function of the central nervous system. R-Ras, a member of the Ras family of small GTPases, plays crucial roles in the regulation of axonal morphology, including outgrowth, branching, and guidance. GTP-bound activated R-Ras reorganizes actin filaments and microtubules through interactions with its downstream effectors, leading to the precise control of axonal morphology. Read More

View Article

Download full-text PDF

Source
https://linkinghub.elsevier.com/retrieve/pii/S24055808183027
Publisher Site
http://dx.doi.org/10.1016/j.bbrep.2018.11.011DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6295856PMC
March 2019
9 Reads

Genistein, daidzein, and resveratrols stimulate PGC-1β-mediated gene expression.

Biochem Biophys Rep 2019 Mar 6;17:51-55. Epub 2018 Dec 6.

Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Kyoto, Japan.

PGC-1β is a transcriptional co-activator of nuclear receptors such as the estrogen receptor-related receptor (ERR). Transgenic overexpression of PGC-1β in mice increases energy expenditure and suppresses high-fat diet-induced obesity. In this study, we screened various food-derived and natural compounds using a reporter assay system to measure the transcriptional activity of PGC-1β. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbrep.2018.11.009DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6295599PMC

Elongation of Müllerian ducts and connection to urogenital sinus determine the borderline of uterine and vaginal development.

Biochem Biophys Rep 2019 Mar 30;17:44-50. Epub 2018 Nov 30.

Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science, 6-3-1 Niijuku, Katsushika-ku, Tokyo 125-8585, Japan.

In female mice, proximal, middle and caudal Müllerian ducts (MDs) differentiate into oviduct, uterus and vagina, respectively. The fates of female reproductive tract epithelia are determined by the mesenchyme. However, the mesenchymal fate determination system is still unclear. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbrep.2018.10.013DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6279966PMC

A serological marker of the N-terminal neoepitope generated during LOXL2 maturation is elevated in patients with cancer or idiopathic pulmonary fibrosis.

Biochem Biophys Rep 2019 Mar 30;17:38-43. Epub 2018 Nov 30.

Nordic Bioscience A/S, Biomarkers and Research, Herlev, Denmark.

Objectives: Lysyl oxidase like 2 (LOXL2) is associated with poor prognosis in idiopathic pulmonary disease (IPF) and cancer. We developed an Enzyme-linked immunosorbent assay (ELISA) targeting the LOXL2 neo-epitope generated through the release of the signal peptide during LOXL2 maturation.

Design And Methods: An ELISA targeting the N-terminal site of the human LOXL2 was developed including technical optimization and validation steps. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbrep.2018.11.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6276730PMC
March 2019
5 Reads

Osteoblastic differentiation improved by bezafibrate-induced mitochondrial biogenesis in deciduous tooth-derived pulp stem cells from a child with Leigh syndrome.

Biochem Biophys Rep 2019 Mar 28;17:32-37. Epub 2018 Nov 28.

Section of Oral Medicine for Children, Division of Oral Health, Growth and Development, Faculty of Dental Science, Kyushu University, 3-1-1 Maidashi, Higashi-Ku, Fukuoka 812-8582, Japan.

Leigh syndrome is a highly heterogeneous condition caused by pathological mutations in either nuclear or mitochondrial DNA regions encoding molecules involved in mitochondrial oxidative phosphorylation, in which many organs including the brain can be affected. Among these organs, a high incidence of poor bone health has been recognized in primary mitochondrial diseases including Leigh syndrome. However, the direct association between mitochondrial dysfunction and poor bone health has not been fully elucidated. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbrep.2018.11.003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6262801PMC
March 2019
1 Read

A LEA model peptide protects the function of a red fluorescent protein in the dry state.

Biochem Biophys Rep 2019 Mar 26;17:27-31. Epub 2018 Nov 26.

Center for Biological Resources and Informatics, Tokyo Institute of Technology, B-62 4259, Nagatsuta-cho, Midori-ku, Yokohama 226-8501, Japan.

We tested whether a short model peptide derived from a group 3 late embryogenesis abundant (G3LEA) protein is able to maintain the fluorescence activity of a red fluorescent protein, mKate2, in the dry state. The fluorescence intensity of mKate2 alone decreased gradually through repeated dehydration-rehydration treatments. However, in the presence of the LEA model peptide, the peak intensity was maintained almost perfectly during such stress treatments, which implies that the three dimensional structure of the active site of mKate2 was protected even under severe desiccation conditions. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbrep.2018.11.006DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6259040PMC
March 2019
1 Read

The mouse-canine chimeric anti-dog podoplanin antibody P38B exerts antitumor activity in mouse xenograft models.

Biochem Biophys Rep 2019 Mar 24;17:23-26. Epub 2018 Nov 24.

Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8575, Japan.

Podoplanin (PDPN) is a type I transmembrane heavily glycosylated sialoglycoprotein that is expressed in normal tissues such as pulmonary type I alveolar cells, renal podocytes, and lymphatic endothelial cells. PDPN overexpression in cancerous tissue is associated with hematogenous metastasis through interactions with the C-type lectin-like receptor 2 (CLEC-2). Previously, we have reported the development of a mouse monoclonal antibody (mAb), PMab-38 (IgG, kappa) against dog PDPN (dPDPN). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbrep.2018.11.005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6260363PMC
March 2019
3 Reads

Promoter methylation cooperates with SNPs to modulate transcription and alter UC risk.

Biochem Biophys Rep 2019 Mar 20;17:17-22. Epub 2018 Nov 20.

Department of Gynaecology and Obstetrics, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, Guangdong, China.

Single-nucleotide polymorphisms (SNPs) located in the promoter region of the receptor for advanced glycation end products () gene have been linked to the activity of RAGE. However, contrary to our expectation, we previously detected no correlation between SNPs within the RAGE promoter and ulcerative colitis (UC) risk in a case-control study. Here, we investigated the methylation of the promoter and analyzed the collective contribution of methylation and SNPs to UC risk. Read More

View Article

Download full-text PDF

Source
https://linkinghub.elsevier.com/retrieve/pii/S24055808183008
Publisher Site
http://dx.doi.org/10.1016/j.bbrep.2018.11.001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6260414PMC
March 2019
14 Reads

Effects of repetitive transcranial magnetic stimulation on ER stress-related genes and glutamate, γ-aminobutyric acid and glycine transporter genes in mouse brain.

Biochem Biophys Rep 2019 Mar 12;17:10-16. Epub 2018 Nov 12.

Clinical Immunology, Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan.

Repetitive transcranial magnetic stimulation (rTMS) is an emerging therapy for the treatment of psychiatric disorders. However, the mechanisms underlying the therapeutic effects of rTMS are still unclear, limiting its optimisation. Lasting effects suggest changes in disease-related genes, so we conducted gene chip and qRT-PCR analyses of genes associated with psychiatric diseases in the mouse brain at various times following 1, 20, 30 or 40 days of rTMS. Read More

View Article

Download full-text PDF

Source
https://linkinghub.elsevier.com/retrieve/pii/S24055808173017
Publisher Site
http://dx.doi.org/10.1016/j.bbrep.2018.10.015DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6234257PMC
March 2019
8 Reads

Evaluation and optimisation of unnatural amino acid incorporation and bioorthogonal bioconjugation for site-specific fluorescent labelling of proteins expressed in mammalian cells.

Biochem Biophys Rep 2019 Mar 6;17:1-9. Epub 2018 Nov 6.

Department of Biochemistry, Genetics and Microbiology, Institute of Microbiology, Single-Molecule Biochemistry Lab, University of Regensburg, Universitätsstraße 31, 93053 Regensburg, Germany.

Many biophysical techniques that are available to study the structure, function and dynamics of cellular constituents require modification of the target molecules. Site-specific labelling of a protein is of particular interest for fluorescence-based single-molecule measurements including single-molecule FRET or super-resolution microscopy. The labelling procedure should be highly specific but minimally invasive to preserve sensitive biomolecules. Read More

View Article

Download full-text PDF

Source
https://linkinghub.elsevier.com/retrieve/pii/S24055808183017
Publisher Site
http://dx.doi.org/10.1016/j.bbrep.2018.10.011DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6226565PMC
March 2019
23 Reads

The calcium sensitizer drug MCI-154 binds the structural C-terminal domain of cardiac troponin C.

Biochem Biophys Rep 2018 Dec 1;16:145-151. Epub 2018 Nov 1.

Department of Medicine, University of Alberta, Edmonton, Alberta, Canada T6G 2R3.

The compound MCI-154 was previously shown to increase the calcium sensitivity of cardiac muscle contraction. Using solution NMR spectroscopy, we demonstrate that MCI-154 interacts with the calcium-sensing subunit of the cardiac troponin complex, cardiac troponin C (cTnC). Surprisingly, however, it binds only to the structural C-terminal domain of cTnC (cCTnC), and not to the regulatory N-terminal domain (cNTnC) that determines the calcium sensitivity of cardiac muscle. Read More

View Article

Download full-text PDF

Source
https://linkinghub.elsevier.com/retrieve/pii/S24055808183005
Publisher Site
http://dx.doi.org/10.1016/j.bbrep.2018.10.012DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6218639PMC
December 2018
18 Reads

Domain unfolding of monoclonal antibody fragments revealed by non-reducing SDS-PAGE.

Biochem Biophys Rep 2018 Dec 31;16:138-144. Epub 2018 Oct 31.

Department of Pharmacology and Systems Physiology, College of Medicine, University of Cincinnati, 231 Albert Sabin Way, Cincinnati, OH 45267-0575, United States.

Monoclonal antibodies and derived fragments are used extensively both experimentally and therapeutically. Thorough characterization of such antibodies is necessary and includes assessment of their thermal and storage stabilities. Thus, assessment of the underlying conformational stabilities of the antibodies is also important. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbrep.2018.10.004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6218646PMC
December 2018
1 Read

Doublecortin-like kinase 1 compromises DNA repair and induces chromosomal instability.

Biochem Biophys Rep 2018 Dec 30;16:130-137. Epub 2018 Oct 30.

Department of Medical Biochemistry, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo 113-8519, Japan.

Doublecortin-like kinase 1 (DCLK1) is a serine/threonine-kinase with two doublecortin (DCX) domains. DCLK1 is associated with microtubules DCX domains and regulates microtubule polymerization. DCLK1 is known to be expressed in cancer stem cells and provides cancer cells with tumor-initiating capacity. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bbrep.2018.10.014DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6216093PMC
December 2018
1 Read