1,248 results match your criteria Biochemistry And Molecular Biology Education[Journal]


Making it stick: A CURE designed to introduce students to the scientific process and the host response to foreign materials.

Biochem Mol Biol Educ 2019 Apr 19. Epub 2019 Apr 19.

Department of Biology, Penn State University, Lehigh Valley, Center Valley, Pennsylvania 18034.

Often overlooked in many molecular cell biology laboratory and cell culture courses, suspension cells represent an important aspect of molecular cell biology and cell culture. Most primary cell cultures and cell lines are adherent cells which grow in monolayers on surfaces. However, other cells such as hematopoietic cells, certain tumor cells, and cells of the immune system are suspension cells which are anchorage-independent which grow and divide in solution. Read More

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http://dx.doi.org/10.1002/bmb.21248DOI Listing

Google classroom as a learning management system to teach biochemistry in a medical school.

Authors:
Sambit Dash

Biochem Mol Biol Educ 2019 Apr 17. Epub 2019 Apr 17.

Department of Biochemistry, Melaka Manipal Medical College, Manipal Academy of Higher Education (MAHE), Karnataka, India.

Medical education has adopted various e-learning technologies to its aid. Addition of Google Classroom, introduced in 2014, as a Learning Management System (LMS) has provided a basic, easy to use platform. This study tested its efficacy in teaching a biochemistry module to first year MBBS students in an Indian medical school. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/bmb.21246
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http://dx.doi.org/10.1002/bmb.21246DOI Listing
April 2019
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Greetings from foodland: Teaching biochemistry to BS students in food-related courses in Italy.

Biochem Mol Biol Educ 2019 Apr 15. Epub 2019 Apr 15.

Department of Food, Environmental, and Nutritional Sciences (DeFENS), University of Milan, Milan, Italy.

Biochemistry has always been a mandatory topic within BS courses aimed at food science students at the University of Milan, namely: Food Science & Technology and Catering Sciences. Addressing biochemistry topics in this peculiar setting requires: (i) specific focus on topics that are seldom considered in courses offered in bio-medical curricula; (ii) close integration with other area disciplines, such as food biotechnology; (iii) ad hoc design of laboratory classes; and (iv) an array of elective courses covering specific aspects of biochemistry. In this context, for example, protein chemistry is presented by using food proteins of known structure and discussed in terms of structural features in the raw materials and of structural and chemical modifications occurring upon processing. Read More

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http://dx.doi.org/10.1002/bmb.21245DOI Listing

Exploring the molecular genetic foundations of cancer biology and how biomedical advances are made in an advanced undergraduate course.

Biochem Mol Biol Educ 2019 Apr 15. Epub 2019 Apr 15.

Department of Biochemistry, University of Missouri, Columbia, Missouri, 65211.

We describe an advanced, inquiry driven undergraduate course in Cancer Biology that combines faculty lectures typical of undergraduate courses with literature-driven discussions typical of graduate courses. As a capstone course, one goal of this course is to integrate knowledge from previous coursework in physiology, cell and molecular biology, genetics, and chemistry, so that students acquire a state-of-the-art understanding of cancer and cancer treatment. A related goal is for students to learn, from the primary literature, how science is performed and how new scientific knowledge is used to improve cancer treatment. Read More

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http://dx.doi.org/10.1002/bmb.21247DOI Listing

Describing teacher conceptions of technology in authentic science inquiry using technological pedagogical content knowledge as a lens.

Biochem Mol Biol Educ 2019 Apr 9. Epub 2019 Apr 9.

Agricultural and Biological Engineering, Technology Leadership and Innovation, Purdue University, West Lafayette, Indiana.

Technology has become an indispensable component of both modern life science and life science education. However, due to inadequate knowledge and experience, teachers are often unable to include technology essential for practicing science in their teaching. In this regard, professional development workshops for in-service and preservice teachers are beneficial. Read More

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http://dx.doi.org/10.1002/bmb.21242DOI Listing

Invisible signals from the underground: A practical method to investigate the effect of microbial volatile organic compounds emitted by rhizobacteria on plant growth.

Biochem Mol Biol Educ 2019 Apr 9. Epub 2019 Apr 9.

Departamento de Biología Molecular, Universidad Nacional de Río Cuarto, RN36 601, Río Cuarto, Córdoba, Argentina.

Rhizobacteria that colonize plant roots and promote plant growth are referred to as plant growth-promoting rhizobacteria, and this can stimulate plant growth either indirectly or directly. Volatile organic compounds (VOCs) emitted by rhizobacteria have the capacity to promote plant growth as well as perform biocontrol of fungal pathogens. The microbial volatile organic compounds (mVOCs) are characterized by a low molecular weight and a high vapor pressure, which facilitate evaporation and diffusion at normal temperatures and at above-ground and below-ground pressures. Read More

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http://dx.doi.org/10.1002/bmb.21243DOI Listing

Individual identification and correction of mistakes in statements of biochemical significance: An effective learning process for graduate medical students.

Biochem Mol Biol Educ 2019 Mar 29. Epub 2019 Mar 29.

Department of Biochemistry, PSG Institute of Medical Sciences and Research, Coimbatore, Tamil Nadu 641 004, India.

Focused self-directed learning process needs to be promoted at present among graduate medical students in view of the information load and limited time available with them. In this study, comparison of the effect of identifying and correcting the mistakes in a given set of wrong statements with the effect of studying correct statements individually by graduate medical students on their understanding of the topic "Cancer Biology" was carried out. At the end of regular teaching module, students were asked to identify the mistakes in a set of seven incorrect statements and correct them individually without peer consultation. Read More

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http://dx.doi.org/10.1002/bmb.21241DOI Listing

Plagiarism or Not? Investigation of Turnitin®-Detected Similarity Hits in Biology Laboratory Reports.

Biochem Mol Biol Educ 2019 Mar 28. Epub 2019 Mar 28.

Section of Cell and Developmental Biology, Division of Biological Sciences, University of California, San Diego, La Jolla, California, 92093.

In undergraduate biology laboratory courses, laboratory reports can be a useful tool for teaching scientific writing, integration of source material, and information literacy; however, these teaching objectives are at times undermined by students' plagiarism. Laboratory instructors often use similarity-matching software to detect plagiarism in laboratory reports, yet similarity hits detected with such software remain poorly characterized. In the upper division molecular biology laboratory course described here, Turnitin® routinely detected dozens of similarity hits in laboratory reports. Read More

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http://dx.doi.org/10.1002/bmb.21236DOI Listing

Bacterial Cellobiose Metabolism: An Inquiry-Driven, Comprehensive Undergraduate Laboratory Teaching Approach to Promote Investigative Learning.

Biochem Mol Biol Educ 2019 Mar 28. Epub 2019 Mar 28.

Department of Chemistry and Biochemistry, Eastern Illinois University, Charleston, Illinois, 61920.

Technique-centered biochemistry or molecular biology undergraduate laboratory curricula do not offer significant opportunities for thoughtful, in-depth exploration of the science to foster investigative learning. To demonstrate inclusion of inquiry-driven laboratory experiments into the undergraduate biochemistry and molecular biology curricula, a comprehensive set of laboratory experiments, covering several principles of biochemistry and molecular biology, have been developed under a single theme. The laboratory curriculum described here comprehensively investigates bacterial cellobiose metabolism using multiple biochemical, molecular biological (RNA isolation, RT-PCR, PCR, and enzyme assay), and analytical techniques (High Performance Liquid Chromatography, NMR, spectrophotometry, and thin-layer chromatography) to explore the principles of metabolomics and genomics in a single undergraduate laboratory course setting using Caulobacter crescentus as the model organism. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/bmb.21237
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http://dx.doi.org/10.1002/bmb.21237DOI Listing
March 2019
3 Reads

"Seeing is Believing." Enhancing student engagement with dynamic protein-model visualization.

Biochem Mol Biol Educ 2019 Mar 28. Epub 2019 Mar 28.

Mechanisms in Cell Biology and Disease Research Group, School of Pharmacy and Medical Sciences, University of South Australia Cancer Research Institute, Adelaide, South Australia, 5000, Australia.

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http://dx.doi.org/10.1002/bmb.21238DOI Listing

When both K and V are altered, Is the enzyme inhibited or activated?

Biochem Mol Biol Educ 2019 Mar 25. Epub 2019 Mar 25.

Department of Chemistry, Willamette University, Salem, Oregon, 97301.

Enzyme activators lower K (the Michaelis constant) and/or raise V (the asymptotic reaction velocity at infinite substrate concentration); conversely, inhibitors raise K and/or lower V . But what if an effector moves both K and V in the same direction? Uncompetitive inhibitors, which decrease both K and V by the same factor, are the most common example of this. A less well-known example occurs often when crowding agents are added to the buffer in order to mimic the environment commonly encountered in vivo. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/bmb.21235
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http://dx.doi.org/10.1002/bmb.21235DOI Listing
March 2019
2 Reads

Understanding Plant Nitrogen Nutrition through a Laboratory Experiment.

Biochem Mol Biol Educ 2019 Mar 25. Epub 2019 Mar 25.

Grupo de Biología Molecular y Biotecnología (BIO-114), Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias, Universidad de Málaga, Campus Universitario de Teatinos s/n, E-29071, Málaga, Spain.

Plant nitrogen nutrition is an essential topic in biology that should be included in scientific education. Nitrogen availability is one of the primary limiting factors for plant growth, and these organisms are the primary support for the pluricellular terrestrial life, since they are the fundamental group of autotrophs. For this reason, the use of nitrogen fertilizers is in the basis of the Green Revolution that led to an extraordinary increase in the food production during the twentieth century. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/bmb.21239
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http://dx.doi.org/10.1002/bmb.21239DOI Listing
March 2019
6 Reads

Student Understanding of DNA Structure-Function Relationships Improves from Using 3D Learning Modules with Dynamic 3D Printed Models.

Biochem Mol Biol Educ 2019 Mar 21. Epub 2019 Mar 21.

Department of Biochemistry, University of Nebraska, Lincoln, Nebraska, 68588-0664.

Understanding the relationship between molecular structure and function represents an important goal of undergraduate life sciences. Although evidence suggests that handling physical models supports gains in student understanding of structure-function relationships, such models have not been widely implemented in biochemistry classrooms. Three-dimensional (3D) printing represents an emerging cost-effective means of producing molecular models to help students investigate structure-function concepts. Read More

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http://dx.doi.org/10.1002/bmb.21234DOI Listing
March 2019
4 Reads

Analysis of kombucha to teach biochemical concepts and techniques to undergraduate students.

Biochem Mol Biol Educ 2019 Mar 20. Epub 2019 Mar 20.

Department of Chemistry and Physics, Indiana State University, Terre Haute, Indiana, 47809.

Laboratory exercises for undergraduate biochemistry students are described in which changes in sugar content during fermentation of the trendy beverage kombucha are analyzed by three methods: thin layer chromatography, a 3,5-dinitrosalicylic acid assay, and a standard commercial blood glucose meter. Each of the three analyses can be completed in a typical laboratory session lasting two to three hours. The exercises are designed to reinforce concepts typically covered in an undergraduate biochemistry course as well as to teach a variety of laboratory techniques. Read More

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http://dx.doi.org/10.1002/bmb.21240DOI Listing

Introducing Programming Skills for Life Science Students.

Biochem Mol Biol Educ 2019 Mar 12. Epub 2019 Mar 12.

Laboratory of Bioinformatics and Systems (LBS), Department of Computer Science, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.

The advent of the high-throughput next-generation sequencing produced a large number of biological data. Knowledge discovery from the huge amount of available biological data requires researchers to develop solid skills in biology and computer science. As the majority of the Bioinformatics professionals are either computer science or life sciences graduates, to teach biology skills to computer science students and computational skills to life science students has become usual. Read More

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http://dx.doi.org/10.1002/bmb.21230DOI Listing

Testing the test: Are exams measuring understanding?

Biochem Mol Biol Educ 2019 Mar 7. Epub 2019 Mar 7.

Division of Biological Sciences, Section of Cell and Developmental Biology, University of California San Diego, La Jolla, California, 92093.

Grades in undergraduate science, technology, engineering, and mathematics (STEM) courses are distributed under the assumption that high-performing students have a strong understanding of the material. Similarly, the STEM education literature often presents exam performance as equivalent to understanding. Despite these assumptions, we have little knowledge regarding student thinking in relation to exam scores. Read More

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http://dx.doi.org/10.1002/bmb.21231DOI Listing

Application of case study to introduce medical students to molecular biology techniques used in HIV diagnostics.

Biochem Mol Biol Educ 2019 Mar 5. Epub 2019 Mar 5.

Chair of Medical Biochemistry, Jagiellonian University Medical College, Kraków, Poland.

Diagnostic molecular biology is a fast developing discipline of laboratory medicine widely used in numerous medical branches such as oncology, hematology, immunology, internal medicine, or infectious diseases, which will certainly have a major impact on clinical medicine in the near future. Nowadays, educational process is forced to face the quickly growing overflow of easily accessible data and properly guide the students not to be lead astray in the information chaos. Hence, in view of the foregoing, it appears obvious that modern medical education should put particular stress on selective acquiring, interpreting, and applying integrated multidisciplinary knowledge rather than on just absorbing and memorizing huge amount of scattered information. Read More

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http://dx.doi.org/10.1002/bmb.21232DOI Listing
March 2019
5 Reads

Basic skills examination in a biochemical practical training program for undergraduate students.

Biochem Mol Biol Educ 2019 Mar 4. Epub 2019 Mar 4.

Department of Biochemistry, Hyogo College of Medicine, 1-1 Mukogawa-cho, Nishinomiya, Hyogo, 663-8501, Japan.

University lectures are mainly passive in nature, and there are few subjects in which students need to learn and function independently. Tutorial education and related activities at universities that specialize in medical and pharmaceutical training have been actively carried out, and lectures in conjunction with practical skills are gradually being developed, although progress has been slow in this area. In past years, our biochemistry practice classes have been evaluated in reports dealing with experiments and written examinations, as is done in other universities. Read More

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http://dx.doi.org/10.1002/bmb.21229DOI Listing
March 2019
4 Reads

Liposome permeability probed by laser light scattering.

Biochem Mol Biol Educ 2019 Feb 27. Epub 2019 Feb 27.

Department of Chemistry, Willamette University, Salem, Oregon, 97301.

We have developed a laboratory project in which students prepare liposomes, expose them to hyperosmotic and hypoosmotic solutions, and follow the resulting shrinking and swelling (respectively) with laser light scattering. Each light intensity transient can be fit to an exponential decline or rise, with the decay constant (k) and the amplitude (ΔV ) being indirectly related to the kinetics and thermodynamics (respectively) of transmembrane osmotic flux. Students vary the experimental system by changing the types and concentrations of osmolytes such as alcohols, amides, and salts. Read More

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http://doi.wiley.com/10.1002/bmb.21226
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http://dx.doi.org/10.1002/bmb.21226DOI Listing
February 2019
7 Reads

Innovative Comprehensive Experiments to Study the Morphological Changes of Apoptotic Cells for Undergraduate Education.

Biochem Mol Biol Educ 2019 Feb 27. Epub 2019 Feb 27.

Department of Cell Biology, School of Basic Medical Sciences, Peking University, Beijing, 100191, China.

This innovative comprehensive experiment is a compulsory course for third-year undergraduate students in the eight-year training program leading to a Doctor of Medicine degree or Doctor of Philosophy degree at Peking University Health Science Center (PKUHSC). There is a critical educational need to prepare these students for the increasing accessibility of research experience. This experimental curriculum of medical cell biology was developed to fulfill such a requirement, which consists of three experiments, including the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide tetrazolium) assay and the immunofluorescence and comet assays to observe antitumor drug-induced cell apoptosis. Read More

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http://doi.wiley.com/10.1002/bmb.21228
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http://dx.doi.org/10.1002/bmb.21228DOI Listing
February 2019
5 Reads

A course-based undergraduate research experience in biochemistry that is suitable for students with various levels of preparedness.

Authors:
Shameka J Shelby

Biochem Mol Biol Educ 2019 Feb 22. Epub 2019 Feb 22.

Department of Chemistry, Biochemistry, and Physics, Florida Southern College, Lakeland, Florida 33801.

Due to resource limitations at predominantly undergraduate institutions, research opportunities for non-senior students can be limited. To provide opportunities for a variety of students to gain exposure to research, a course-based undergraduate research experience (CURE) was designed and conducted. Coupled inquiry was used to allow underclassmen and upperclassmen to participate. Read More

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http://dx.doi.org/10.1002/bmb.21227DOI Listing
February 2019

An innovative bioanalytical research project course to train undergraduate students on liquid chromatography-mass spectrometry.

Biochem Mol Biol Educ 2019 Feb 21. Epub 2019 Feb 21.

Department of Chemistry, College of Arts and Sciences, Khalifa University, Abu Dhabi, UAE.

Active-learning strategies such as undergraduate research courses and course-based undergraduate research experiences (CUREs), which engage students in the practical experiments, have been reported as efficient ways for effective teaching and training of graduating students. Recently, many practical examples have been published in various fields to develop critical skills needed by undergraduate students for graduate school or the workforce; however, very few examples have been published for bioanalytical topics, specifically, Liquid Chromatography-Mass Spectrometry (LC-MS-MS). Herein, we describe an innovative undergraduate research course that was used to train well-prepared graduating seniors on LC-MS-MS and in developing a sensitive method for detecting vitamin B9 (folic acid) levels in different milk samples. Read More

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http://dx.doi.org/10.1002/bmb.21225DOI Listing
February 2019

Bibliometric analysis of the brazilian periodical "journal of biochemistry education".

Biochem Mol Biol Educ 2019 Feb 19. Epub 2019 Feb 19.

Programa de Educação, Gestão e Difusão de Biociências, Instituto de Bioquímica Médica Leopoldo de Meis, Prédio do CCS, Bloco E-27B, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ 21941-902, Brazil.

The bibliometric analysis of the Brazilian periodical "Journal of Biochemistry Education" (JBE) covered the 117 articles published in 15 volumes in the period 2001-2017. Our results showed a positive trend in JBE publications with a significant increase in the number of articles since 2014, which can be related to the increase in research groups working in this area. The Southeast region of Brazil was the most productive one mainly due to the contribution of papers from institutions located in the State of São Paulo. Read More

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http://dx.doi.org/10.1002/bmb.21220DOI Listing
February 2019

Virtual laboratory simulation in the education of laboratory technicians-motivation and study intensity.

Biochem Mol Biol Educ 2019 Feb 12. Epub 2019 Feb 12.

Department of Design and Communication, University of Southern Denmark, Kolding, Denmark 6000.

This study presents an evaluation of virtual laboratory simulation for educational use in the AP Degree Programme in Chemical and Biotechnical Science at University College Copenhagen in Denmark. The purpose was to test if, and how, virtual laboratory simulation could be applied to a practically oriented education such as the education of laboratory technicians-the aim being to motivate students and improve the education with new teaching tools. The study investigated how specific virtual lab simulation cases (Labster-cases) may stimulate motivation, study intensity, and learning among laboratory technician students. Read More

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http://dx.doi.org/10.1002/bmb.21221DOI Listing
February 2019

Demonstration of allelopathy of horseradish root extract on lettuce seed.

Biochem Mol Biol Educ 2019 Feb 12. Epub 2019 Feb 12.

Division of Plant and Soil Sciences, West Virginia University, Morgantown, West Virginia, 26505.

Allelopathy plays crucial roles in invasive plant viability and agricultural production systems. However, there is no well-established hands-on learning activity to teach the concept of allelopathy. Nor is there an activity which allows students to gain knowledge about glucosinolates and their corresponding enzyme, myrosinase, which are present in almost all Brassica crops. Read More

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http://dx.doi.org/10.1002/bmb.21219DOI Listing
February 2019

A Research-inspired biochemistry laboratory module - combining expression, purification, crystallization, structure-solving, and characterization of a flavodoxin-like protein.

Biochem Mol Biol Educ 2019 Feb 11. Epub 2019 Feb 11.

Department of Biosciences, Section for Biochemistry and Molecular Biology, University of Oslo, NO-0316 Oslo, Norway.

Many laboratory courses consist of short and seemingly unconnected individual laboratory exercises. To increase the course consistency, relevance, and student engagement, we have developed a research-inspired and project-based module, "From Gene to Structure and Function". This 2. Read More

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http://dx.doi.org/10.1002/bmb.21218DOI Listing
February 2019
2 Reads

Commentary: Innovation in the Biochemistry/Molecular Biology Laboratory.

Biochem Mol Biol Educ 2019 Feb 9. Epub 2019 Feb 9.

Department of Chemistry and Biochemistry, Hope College, Holland, Michigan.

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http://dx.doi.org/10.1002/bmb.21217DOI Listing
February 2019

Development and Validation of the Biotechnology Literacy Test.

Biochem Mol Biol Educ 2019 Mar 8;47(2):179-188. Epub 2019 Feb 8.

Department of Special Education, Hacettepe University, Ankara, Turkey.

In this study, a 'biotechnology literacy test' was developed to determine the biotechnology literacy of prospective science teachers, and its validity and reliability were determined. For this purpose, 42 items were prepared by considering Bybee's scientific literacy classifications (nominal, functional, procedural, and multidimensional). The experts were asked to rate each item for content validity. Read More

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http://doi.wiley.com/10.1002/bmb.21216
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http://dx.doi.org/10.1002/bmb.21216DOI Listing
March 2019
6 Reads

Not all has been said about glucose oxidase/peroxidase: New pedagogical uses for a classical and robust undergraduate laboratory experiment.

Biochem Mol Biol Educ 2019 Feb 8. Epub 2019 Feb 8.

Facultad de Ciencias, Biología Molecular y Bioquímica, Campus de Teatinos, Universidad de Málaga, Andalucía Tech, 29071 Málaga, Spain.

The enzymatic determination of glucose in soft drinks, based on the glucose concentration measurement by means of the coupled reactions of glucose oxidase and peroxidase, has been implemented at the University of Malaga and optimized according to the Biochemistry and Chemistry undergraduate students' results and feedback throughout the last few years. This traditional and robust laboratory practical has been reformed, in the light of inquiry-based and interdisciplinary learning approaches, in order to optimize the students' formative achievements that now are not restricted to the learning of enzymology, but also integrates cross-curricular knowledge from chemistry and mathematics. In this experiment, inexpensive and feasible to be carried out in a single laboratory session, students have to make the decision of what method is the most suitable for a given analytical problem, anticipating situations that they will probably face throughout their professional careers. Read More

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http://dx.doi.org/10.1002/bmb.21224DOI Listing
February 2019

Undergraduate lab series using the K562 human leukemia cell line: Model for cell growth, death, and differentiation in an advanced cell biology course.

Biochem Mol Biol Educ 2019 Feb 6. Epub 2019 Feb 6.

Department of Biology, Fairfield University, Fairfield, Connecticut, 06824.

This sequence of labs was developed for an upper level undergraduate cell biology course at Fairfield University. The labs are based on the use of the K562 human erythroleukemia cell line, a model system that is exceptionally amenable to an undergraduate cell biology lab course due to its ease of maintenance and propagation and usefulness for studies of growth, death, and differentiation. The sequence of labs is conducted over a 6-week period, following a series of weekly cell biology labs covering basic cell and molecular biology techniques. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/bmb.21222
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http://dx.doi.org/10.1002/bmb.21222DOI Listing
February 2019
7 Reads

Evaluating the appropriateness of AccessMedicine in integrated biochemistry learning for chinese medical students.

Biochem Mol Biol Educ 2019 Feb 6. Epub 2019 Feb 6.

Department of Biochemistry and Molecular Biology, Department of Regenerative Medicine, Tongji University School of Medicine, Shanghai, 200092, China.

Recently, Chinese medical education has exhibited a shifting trend from teacher-centered instruction to student-centered learning, which usually includes a self-directed learning process. Providing students with high-quality and appropriate supplemental resources is necessary for their self-directed learning. This study aimed to investigate the appropriateness of AccessMedicine, an online medical resource, for first-year medical students at Tongji University in China. Read More

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http://dx.doi.org/10.1002/bmb.21223DOI Listing
February 2019

A Designed Experiment: Polymorphism Analysis of Angiotensin-Converting Enzyme Gene from Human Buccal Epithelial Cells.

Biochem Mol Biol Educ 2019 Mar 29;47(2):168-174. Epub 2019 Jan 29.

Department of Biochemistry and Molecular Biology Laboratory, Teaching Laboratory Center of Medicine and Life Science, Tongji University School of Medicine, Shanghai, China.

For medical students, we combine the laboratory practice with clinical applications by developing biochemical and molecular biology experiments. In this experiment, students first collect their own buccal epithelial cells by a noninvasive mouthwash method. Then, they extract genomic DNA and perform polymerase chain reaction (PCR) to amplify angiotensin-converting enzyme (ACE) gene using genomic DNA as a template. Read More

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http://dx.doi.org/10.1002/bmb.21215DOI Listing

An undergraduate laboratory on RNA sequencing analysis of bacterial gene expression.

Biochem Mol Biol Educ 2019 Mar 25;47(2):161-167. Epub 2019 Jan 25.

Biology Department, State University of New York at Geneseo, Geneseo, New York 14454.

Next generation sequencing has revolutionized molecular biology and has provided a mechanism for rapid DNA and RNA sequence analysis. Yet, there are few resources to introduce next generation sequencing into the undergraduate biochemistry and molecular biology curriculum. Herein, we describe the design, execution, and assessment of a four-week laboratory for junior and senior undergraduate students that focuses on bacterial gene expression changes detected by RNA sequencing (RNA-seq). Read More

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http://dx.doi.org/10.1002/bmb.21212DOI Listing

OligoCOOL: A mobile application for nucleotide sequence analysis.

Biochem Mol Biol Educ 2019 Mar 25;47(2):201-206. Epub 2019 Jan 25.

Department of Biophysics, School of Biological Sciences, Tarbiat Modares University, Tehran, Iran.

Today smartphones are inseparable parts of modern life and are capable of performing many desktop computers' tasks such as scientific analysis with greater convenience. Here, we present OligoCOOL, which is an Android application for analyzing nucleic sequences. This application enables users to perform several common biomedical analyses for a given nucleotide sequence. Read More

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http://dx.doi.org/10.1002/bmb.21213DOI Listing

It Is Time for a New Direction in Biotechnology Education Research.

Biochem Mol Biol Educ 2019 Mar 25;47(2):189-200. Epub 2019 Jan 25.

Department of Biological and Environmental Sciences, University of Gothenburg, Gothenburg, Sweden.

This manuscript presents a scoping review of Biotechnology Education Research (BTER) over the last 20 years. BTER during this period primarily focused on attitudes and knowledge, typically using research design methods suitable for addressing questions about frequencies, central tendencies, correlations, and so on. However, to guide the development of educational practices that will increase and sustain students' motivation and interest in science in general and biotechnology in particular, BTER will need to adopt research design methods that support the inference of causal relationships from observations. Read More

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http://dx.doi.org/10.1002/bmb.21214DOI Listing
March 2019
9 Reads

An undergraduate laboratory experience using CRISPR-cas9 technology to deactivate green fluorescent protein expression in Escherichia coli.

Biochem Mol Biol Educ 2019 Mar 21;47(2):145-155. Epub 2019 Jan 21.

Department of Biology, Stetson University, DeLand, Florida.

Undergraduates learn that gene editing in diverse organisms is now possible. How targeted manipulation of genes and genomes is utilized in basic science and biomedicine to address biological questions is challenging for undergraduates to conceptualize. Thus, we developed a lab experience that would allow students to be actively engaged in the full process of design, implementation of a gene editing strategy, and interpretation of results within an 8-week lab period of a Genetics course. Read More

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http://dx.doi.org/10.1002/bmb.21206DOI Listing
March 2019
1 Read

Creating custom Foldit puzzles for teaching biochemistry.

Biochem Mol Biol Educ 2019 Mar 14;47(2):133-139. Epub 2019 Jan 14.

Department of Chemistry and Biochemistry and the Knoebel Institute for Healthy Aging, University of Denver, Denver, Colorado, 80208.

The computer game Foldit is currently widely used as a biology and biochemistry teaching aid. Herein, we introduce a new feature of Foldit called "custom contests" that allows educators to create puzzles that fit their curriculum. The effectiveness of the custom contests is demonstrated by the use of five distinct custom contests in an upper-level biochemistry class. Read More

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http://dx.doi.org/10.1002/bmb.21208DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6428574PMC

Teaching methods for microbiology in Saudi Arabia.

Authors:
Fuad Ameen

Biochem Mol Biol Educ 2019 Mar 12;47(2):175-178. Epub 2019 Jan 12.

Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.

Microbiology as an academic discipline that does not have a long history, but the knowledge accumulated over the past three centuries is rather considerable, and courses in microbiology are commonly taught in colleges and universities throughout the world. In this article, many of the methods that can be used for teaching microbiology in a classroom and laboratory are discussed. In brief, these methods are limited only by the basic technical facilities to which one has access as well as one's own imagination and creativity. Read More

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http://doi.wiley.com/10.1002/bmb.21209
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http://dx.doi.org/10.1002/bmb.21209DOI Listing
March 2019
20 Reads

Exploring ligand-protein interaction: A laboratory exercise on herbicide binding to plasma transport protein.

Biochem Mol Biol Educ 2019 Mar 10;47(2):156-160. Epub 2019 Jan 10.

Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia.

A laboratory exercise on the interaction between the herbicide pendimethalin (PM) and goat serum albumin (GSA), a carrier protein present in mammalian blood circulation, is described. Fluorescence spectroscopy was used to study the binding reaction between PM and GSA. Titration of a constant amount of the protein (GSA) with increasing ligand (PM) concentrations produced a consecutive decrease in the protein's fluorescence. Read More

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http://dx.doi.org/10.1002/bmb.21207DOI Listing
March 2019
0.654 Impact Factor

A convenient tool for bivariate data analysis and bar graph plotting with R.

Biochem Mol Biol Educ 2019 Mar 10;47(2):207-210. Epub 2019 Jan 10.

Center for Biochemistry, Institute of Biochemistry I, Medical Faculty, University of Cologne, Cologne, Germany.

The Java software jBar consists of a graphical user interface that allows the user to customize and assemble an included script for R. The scripted R pipeline calculates means and standard errors/deviations for replicates of numerical bivariate data and generates presentations in the form of bar graphs. A two-sided Student's t test is carried out against a user-selected reference and p-values are calculated. Read More

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http://dx.doi.org/10.1002/bmb.21205DOI Listing

Characterization of the recombinant (R)- and (S)-hydroxypropyl-coenzyme M dehydrogenases: A case study to augment the teaching of enzyme kinetics and stereoselectivity.

Authors:
Daniel D Clark

Biochem Mol Biol Educ 2019 Mar 28;47(2):124-132. Epub 2018 Dec 28.

Department of Chemistry and Biochemistry, California State University-Chico, Chico, California, 95929.

A homologous pair of stereospecific NAD-dependent enzymes, (R)- and (S)-hydroxypropyl-coenzyme M dehydrogenase, are part of a bacterial pathway of short-chain alkene and epoxide metabolism. Their discovery and study, which spans multiple publications over more than a decade, is a data rich story that combines both classical and contemporary experimental biochemistry. A subset of the data for characterization of the recombinant enzymes was used as a case study to augment the teaching of enzyme kinetics and stereoselectivity in an undergraduate biochemistry course at California State University-Chico. Read More

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http://dx.doi.org/10.1002/bmb.21202DOI Listing

A Novel Grading Strategy for Team-Based Learning Exercises in a Hands-on Course in Molecular Biology for Senior Undergraduate Underrepresented Students in Medicine Resulted in Stronger Student Performance.

Biochem Mol Biol Educ 2019 Mar 27;47(2):115-123. Epub 2018 Dec 27.

Department of Medical Sciences, Hackensack Meridian School of Medicine at Seton Hall University, Nutley, New Jersey.

We developed a hands-on course in molecular biology for undergraduate underrepresented in medicine (URM) students. To incentivize student preparation for team-based learning (TBL) activities, we implemented a novel grading schema that requires a minimum individual readiness assurance test (iRAT) score to share the team group readiness assurance test (gRAT) score. Fifty-one students participated in this 2-year study and were divided in teams of five or six students that worked throughout the course on a unique, hands-on project and also participated in TBL exercises. Read More

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http://dx.doi.org/10.1002/bmb.21200DOI Listing

Song: I love ketone bodies (to the tune of "shape of you").

Biochem Mol Biol Educ 2019 Mar 27;47(2):211. Epub 2018 Dec 27.

Department of Biochemistry, Medical Sciences Building, University of Western Ontario, London, Ontario, N6A 5C1, Canada.

This song alludes to the main sources of stored energy used by the brain in an extended fasting situation: first glycogen, then proteins (broken down to provide starting material for gluconeogenesis), then fatty acids. Notably for the song, because the brain does not directly take up fatty acids, the liver converts fatty acids to ketone bodies, which are then released into the bloodstream for the brain's benefit. The brain can absorb ketone bodies and convert them to acetyl-CoA, allowing ATP production through the Kreb's cycle and oxidative phosphorylation. Read More

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http://doi.wiley.com/10.1002/bmb.21203
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http://dx.doi.org/10.1002/bmb.21203DOI Listing
March 2019
9 Reads

Inconsistencies in some common terms and notations in enzymology: Textbook examples and suggestions.

Biochem Mol Biol Educ 2019 Mar 25;47(2):140-144. Epub 2018 Dec 25.

International Research Centre for Climate Change Studies, Sathyabama Institute of Science and Technology, Chennai, Tamil Nadu, 600119, India.

A number of terms have been defined and inconsistently used across various texts in enzymology which may attract the attention of undergraduate students and instructors. At some places, inconsistent use results in confusions as well as leads to the misuse of these terms. Although it is difficult to resolve such inconsistencies, attempts have constantly been made by international regulatory forums such as International Union of Biochemistry and Molecular Biology (IUBMB) and other similar organizations to abolish the inconsistencies. Read More

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http://dx.doi.org/10.1002/bmb.21204DOI Listing
March 2019
0.654 Impact Factor

Using fluorescent lipids contributes to the active learning of principles underlying lipid signaling.

Biochem Mol Biol Educ 2019 01 25;47(1):100-105. Epub 2018 Dec 25.

Departamento de Biología Molecular, FCEFQN, Universidad Nacional de Río Cuarto, Córdoba, Argentina.

The concepts of phospholipase activity is often taught in undergraduate biology and biochemistry classes and reinforced in laboratory exercises. However, very rarely does the design of these exercises allow students to directly gain experience in the use of modern instruments such as digital imaging systems and fluorescence spectrophotometers. The laboratory exercise described here involves the use of fluorescent lipids to evaluate phospholipase activity. Read More

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http://dx.doi.org/10.1002/bmb.21201DOI Listing
January 2019

Computational strategy for visualizing structures and teaching biochemistry.

Biochem Mol Biol Educ 2019 01 22;47(1):76-84. Epub 2018 Dec 22.

Laboratório de Antibióticos, Bioquímica, Ensino e Modelagem Molecular (LabiEMol), Instituto de Biologia, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil.

Computational techniques have great potential to improve the teaching-learning. In this work, we used a computational strategy to visualize three-dimensional (3D) structures of proteins and DNA and help the student to comprehend biochemistry concepts such as protein structure and function, substrate, and inhibitors as well as DNA structural features. The practical classes included tutorials to be done in the computer using structures from Protein Data Bank and a free 3D structure visualization software, Swiss PDB Viewer. Read More

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http://doi.wiley.com/10.1002/bmb.21199
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http://dx.doi.org/10.1002/bmb.21199DOI Listing
January 2019
8 Reads

Guided inquiry activity linking thermodynamic parameters of protein unfolding to structure using differential scanning fluorimetry data in the biophysical chemistry classroom.

Biochem Mol Biol Educ 2019 01 22;47(1):67-75. Epub 2018 Dec 22.

Department of Chemistry and Biochemistry, Miami University, Oxford, Ohio, 45056.

Visualizations are useful tools for helping students to understand chemistry concepts at the particulate level. A classroom activity was developed based on learning theory and evidence-based practices, combining protein visualization with thermodynamic parameters from differential scanning fluorimetry (DSF) data analysis. Coding of student responses showed that many students were able to establish the desired connections among protein structure, thermodynamic parameters, and experimental data analysis, while a few did not recognize all the differences between the folded and unfolded forms of the protein. Read More

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http://dx.doi.org/10.1002/bmb.21198DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6329392PMC
January 2019

The effect and influence of undergraduate research on medical undergraduates in China.

Biochem Mol Biol Educ 2019 01 21;47(1):41-50. Epub 2018 Dec 21.

Department of Plastic Surgery, The 3rd Xiangya Hospital of Central South University, Changsha, China.

Undergraduate research (UR) refers to a series of scientific research activities for undergraduates. For the past few years in China, medical colleges and universities have increasingly attached importance on UR, aiming to provide undergraduates with better scientific research conditions, improve their understanding about scientific research and their basic scientific skills. Chinese medical undergraduates participate in scientific research spontaneously. Read More

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http://dx.doi.org/10.1002/bmb.21194DOI Listing
January 2019
3 Reads

A laboratory work to introduce biochemistry undergraduate students to basic enzyme kinetics-alkaline phosphatase as a model.

Biochem Mol Biol Educ 2019 01 21;47(1):93-99. Epub 2018 Dec 21.

Universidad de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas, IQUIFIB, Facultad de Farmacia y Bioquímica, Departamento de Química Biológica, Buenos Aires, Argentina.

Enzyme kinetics is an essential topic in undergraduate Biochemistry courses. A laboratory work that covers the principal basic concepts of enzyme kinetics in steady state is presented. The alkaline phosphatase catalyzed reaction of phenyl-phosphate hydrolysis was studied as a model. Read More

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http://doi.wiley.com/10.1002/bmb.21195
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http://dx.doi.org/10.1002/bmb.21195DOI Listing
January 2019
1 Read

Is learning outcome after team based learning influenced by gender and academic standing?

Biochem Mol Biol Educ 2019 01 20;47(1):58-66. Epub 2018 Dec 20.

Department of Biochemistry, Maulana Azad Medical College, New Delhi, 110002, India.

Team based learning (TBL) is a time tested teaching-learning (T-L) tool involving collaborative learning but has hardly been tested for teaching clinical biochemistry to undergraduate medical students. The present study was designed to (a) compare problem solving skills of first year MBBS students after attending a TBL session on 'organ function test' with that of the students taught the same topic by didactic lecture, (b) assess their perception towards TBL and (c) evaluate if difference in academic standing and gender influence the learning outcome of TBL.One Hundred first professional MBBS students were divided by stratified randomization into two groups. Read More

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http://dx.doi.org/10.1002/bmb.21197DOI Listing
January 2019
0.654 Impact Factor