3,156 results match your criteria Biochemistry And Cell Biology[Journal]


Effect of Caveolin-1 upon Stat3-ptyr705 levels in breast and lung carcinoma cells.

Biochem Cell Biol 2019 Apr 15. Epub 2019 Apr 15.

Queens University, Department of Biomedical and Molecular Sciences, Pathology and Molecular Medicine, and Queen's University Cancer Research Institute,, Kingston, Ontario, Canada ;

We recently demonstrated that Cav1 is a negative regulator of Stat3 activity in mouse fibroblasts and human lung carcinoma SHP77 cells. We now examined whether the cellular context may affect their levels and relationship between them, by assessing Cav1 and Stat3-ptyr705 levels in different lines. In MDA-MB-231, A549 and HaCat cells Cav1 levels were high and Stat3-ptyr705 levels were low, consistent with the notion of a negative effect of the endogenous Cav1 upon Stat3-ptyr705 levels in these lines. Read More

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http://dx.doi.org/10.1139/bcb-2018-0367DOI Listing

40th International Asilomar Chromatin, Chromosomes and Epigenetics Conference.

Biochem Cell Biol 2019 Apr 11. Epub 2019 Apr 11.

University of Victoria , Department of Biochemistry and Microbiology , P.O. Box 3055 , Victoria, British Columbia, Canada , V8W 3P6 ;

The 40th International Asilomar Chromatin, Chromosomes and Epigenetics Conference was held in the Asilomar Conference Grounds, Pacific Groove California, USA on December 6Th-9th 2018. The organizing committee consisted of established scientists in the fields of chromatin and epigenetics: Sally Pasion and Michael Goldman from the Biology Department, San Francisco State University, CA, USA, Philippe Georgel from the Department of Biological Sciences, Marshal University, WV, USA, Juan Ausió from the Biochemistry and Microbiology, University of Victoria, BC, Canada and Christopher Eskiw from the Department of Biochemistry, University of Saskatchewan, SK, Canada. The meeting had two keynote speakers: Jessica Tyler and Jennifer Mitchell and it covered topics on transcription, replication and repair, epigenetics, cell differentiation and disease, telomeres and centromeres and it had two sessions devoted to nuclear and genomic organization. Read More

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http://www.nrcresearchpress.com/doi/10.1139/bcb-2019-0054
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http://dx.doi.org/10.1139/bcb-2019-0054DOI Listing
April 2019
3 Reads

miR-4458 inhibits breast cancer cell growth, migration, and invasion by targeting CPSF4.

Biochem Cell Biol 2019 Apr 10. Epub 2019 Apr 10.

Women's Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital , No. 123, Tian Fei lane, Qinhuai District, Nanjing , Nanjing, China , 210004 ;

Numerous studies have implied that CPSF4 is over-expressed in a large percentage of human lung cancers and identified as a potential oncogene of human lung tumor. Down-regulation of CPSF4 inhibited lung adenocarcinoma cells proliferation and promoted lung adenocarcinoma cells apoptosis. Our previous study also suggested that CPSF4 overexpression was observed in breast cancer, and closely associated with poor prognosis. Read More

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http://www.nrcresearchpress.com/doi/10.1139/bcb-2019-0008
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http://dx.doi.org/10.1139/bcb-2019-0008DOI Listing
April 2019
3 Reads

Impact of double-stranded RNA characteristics on the activation of human 2'-5'-oligoadenylate synthetase 2 (OAS2).

Biochem Cell Biol 2019 Apr 9. Epub 2019 Apr 9.

University of Manitoba, 8664, Winnipeg, Manitoba, Canada ;

Human 2'-5' oligoadenylate synthetases (OAS) are interferon inducible proteins which, upon activation by double-stranded RNA, polymerize ATP into 2'-5' linked oligoadenylates. Here, we probe the RNA cofactor specificity of the two smallest isozymes, OAS1 and OAS2. First, we demonstrate the purification of recombinant OAS2 from human cells and quantified enzymatic activity relative to OAS1. Read More

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http://dx.doi.org/10.1139/bcb-2019-0060DOI Listing

SARS coronavirus protein nsp1 disrupts localization of Nup93 from the nuclear pore complex.

Biochem Cell Biol 2019 Apr 3. Epub 2019 Apr 3.

Furman University, 3628, Chemistry and Biology , 3300 Poinsett Highway , Greenville, South Carolina, United States , 29613-0002 ;

Severe acute respiratory syndrome coronavirus nonstructural protein 1 (nsp1) is a key factor in virus-induced down-regulation of host gene expression. In infected cells, nsp1 engages in a multi-pronged mechanism to inhibit host gene expression by binding to the 40S ribosome to block the assembly of translationally competent ribosome, and then inducing endonucleolytic cleavage and the degradation of host mRNAs. Here, we report a previously undetected mechanism by which nsp1 exploits the nuclear pore complex and disrupts nuclear-cytoplasmic transport of biomolecules. Read More

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http://dx.doi.org/10.1139/bcb-2018-0394DOI Listing
April 2019
2 Reads

Biological activity of Echinops spinosus on inhibition of paracetamol-induced renal inflammation.

Biochem Cell Biol 2019 04 1;97(2):176-186. Epub 2019 Apr 1.

b Botany Department, Faculty of Science, Ain Shams University, Abbassia, 11566, Cairo, Egypt.

This study was designed to evaluate the possible mechanisms through which Echinops spinosus (ES) extract demonstrates nephroprotective effect on the paracetamol acetominophen (N-acetyl-p-aminophenol (APAP)) induced nephrotoxicity in rats. Twenty-four Swiss albino rats were divided into four groups (six rats each). The placebo group was orally administered sterile saline, the APAP group received APAP (200 mg·kg·day i. Read More

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http://dx.doi.org/10.1139/bcb-2018-0212DOI Listing
April 2019
1 Read

Current approaches for RNA labelling to identify RNA-binding proteins.

Biochem Cell Biol 2019 Mar 30. Epub 2019 Mar 30.

University of Lethbridge, 4512, Dept of Chemistry & Biochemistry , 4401 University Drive West , Lethbridge, Alberta, Canada , T1K 3M4 ;

RNA is involved in all domains of life, playing critical roles in a host of gene expression processes, host-defense mechanisms, cell proliferation, and diseases. A critical component in many of these events is the ability for RNA to interact with proteins. Over the past few decades, our understanding of such RNA-protein interactions and their importance has driven the search and development of new techniques for the identification of RNA-binding proteins. Read More

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http://dx.doi.org/10.1139/bcb-2019-0041DOI Listing
March 2019
1 Read

Citicoline protects against lead-induced oxidative injury in neuronal PC12 cells.

Biochem Cell Biol 2019 Mar 29. Epub 2019 Mar 29.

Department of Pharmacology and Toxicology, School of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran, Kerman, Iran (the Islamic Republic of) ;

Lead is a major environmental pollutant, which causes serious adverse effects on biological systems and cells. In this study, we examined the effect of citicoline on lead-induced apoptosis in PC12 cells. PC12 cells were pre-treated with citicoline and then exposed to lead for 48 h. Read More

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http://dx.doi.org/10.1139/bcb-2018-0218DOI Listing

Anti-adipogenesis and antioxidative defense status of Kalasin 2 cultivar peanut sprout crude extract in 3T3-L1 mouse adipocytes.

Biochem Cell Biol 2019 Mar 25. Epub 2019 Mar 25.

Naresuan University, 59212, Biochemistry, Faculty of Medical Science, Phitsanulok, Thailand ;

The aim of this study was to investigate the effects of germinated (GPE) and non-germinated peanut (NGPE) extracts on adipogenesis and oxidative status in normal and oxidative stress-induced 3T3-L1 mouse adipocytes. The treated cells were analysed for cell growth, lipid accumulation, intracellular ROS and the expression of mRNAs and proteins related to adipogenesis and antioxidative defense systems. The results indicated that 9 day-germinated peanut extract (9GPE) reduced lipid contents and mRNA expression of adipogenesis-related genes greater than 1-day germinated (1GPE) and NGPE, respectively. Read More

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http://dx.doi.org/10.1139/bcb-2018-0391DOI Listing
March 2019
1 Read

OTOR in breast carcinoma as a potent prognostic predictor correlates with cell proliferation, migration and invasion.

Authors:
Yu-Fei Wang Jie Han

Biochem Cell Biol 2019 Mar 21. Epub 2019 Mar 21.

Jining NO.1 people's hospital, Jining, China ;

Otoraplin (OTOR), recognized as an important cochlear gene, has a predicted secretion signal peptide sequence and harbors a high degree of cross-species conservation. However, its role in tumor progression is relatively unclear, especially in breast carcinoma (BC). This study aimed to investigate clinicopathologic significance of OTOR in breast infiltrating ductal carcinoma (IDC) with high metastasis and unveil its biological function in BC. Read More

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http://dx.doi.org/10.1139/bcb-2018-0305DOI Listing
March 2019
5 Reads

Annexin A2 expression and partners during epithelial cell differentiation.

Biochem Cell Biol 2019 Mar 18. Epub 2019 Mar 18.

Centre National de la Recherche Scientifique, 27051 , 4 place Jussieu , Paris, France , 75794 ;

The members of the annexin family of calcium- and phospholipid-binding proteins participate in different cellular processes. Annexin A2 binds to S100A10 forming a functional heterotetrameric protein that has been involved in many cellular functions such as exocytosis, endocytosis, cell junction formation and actin cytoskeleton dynamics. Herein, we studied annexin A2 cellular movements and looked for its partners during epithelial cell differentiation. Read More

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http://dx.doi.org/10.1139/bcb-2018-0393DOI Listing

Serum-dependent and independent regulation of PARP2.

Biochem Cell Biol 2019 Mar 17. Epub 2019 Mar 17.

Western University, 6221, Biology, London, Ontario, Canada ;

PARP2 belongs to a family of proteins involved in cell differentiation, DNA damage repair, cellular energy expenditure, chromatin modeling and cell differentiation. In addition to these overlapping functions with PARP1, PARP2 participates in spermatogenesis, T-cell maturation, extraembryonic endoderm formation, adipogenesis, lipid metabolism and cholesterol homeostasis. The function(s) of PARP2 is far from complete, and the mechanism(s) by which the gene and protein are regulated are unknown. Read More

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http://dx.doi.org/10.1139/bcb-2018-0345DOI Listing

Apoptosis induced by synthetic compounds containing a 3,4,5-trimethoxyphenyl fragment against lymphoid immature neoplasms.

Biochem Cell Biol 2019 Mar 8. Epub 2019 Mar 8.

Universidade Federal de Santa Catarina, 28117, Análises Clínicas , Campus Universitário , Florianopolis, Santa Catarina, Brazil , 88040-900 ;

T-cell Acute Lymphoblastic Leukemia is an aggressive hematological malignancy originating from the malignant transformation of progenitor T cells at different stages of development. The treatment causes severe adverse effects and is associated with relapses and high morbidity and mortality rates. The present study aimed to evaluate the cytotoxic activity of 28 new compounds containing 3,4,5-trimethoxyphenyl analogues on hematological neoplastic cells lines. Read More

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http://dx.doi.org/10.1139/bcb-2018-0316DOI Listing
March 2019
2 Reads

Schisandrin B ameliorates high glucose-induced vascular endothelial cells injury by regulating Noxa/Hsp27/NF-κB signaling pathway.

Biochem Cell Biol 2019 Feb 28. Epub 2019 Feb 28.

The First Affiliated Hospital of Guangxi Medical University , No. 6, Shuangyong Road, Nanning 530021, Guangxi Province, PR China , Nanning, China , 530021 ;

Background: To address the molecular mechanism of anti-inflammation effects of Sch B in atherosclerosis, we performed the investigation on injured HMEC-1 and HUVEC-12 cells induced by high glucose (HG).

Methods: Western blotting was performed to detect the protein levels of Hsp27, Noxa, TLR5, p-IκBα and p-p65 in HG induced cells, while ELISA assay was used to analyze the inflammatory cytokines TNF-α, IL-6, MCP-1 and IL-1β in cells with Hsp27 or Noxa stable expression.

Results: Over-expression of Hsp27 up-regulated the inflammatory cytokines and release of IκBα, promoted transportation of p65 into nuclear and finally affected the inflammation process, while Sch B counteracted the up-regulation. Read More

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http://dx.doi.org/10.1139/bcb-2018-0321DOI Listing
February 2019
5 Reads

Differential expression of leaf proteome of tolerant and susceptible maize (Zea mays L.) genotype in response to multiple abiotic stresses.

Authors:
Suphia Rafique

Biochem Cell Biol 2019 Feb 26. Epub 2019 Feb 26.

JAMIA HAMDARD, DEPARTMENT OF BIOTECHNOLOGY, New Delhi, DELHI, India ;

In the present work, tropical maize genotypes were evaluated against the combined stresses (drought x low-N, and waterlogging x low-N) that were set to 30 days old maize seedlings concurrently. Two-dimensional gel electrophoresis (2DE) was used to examine the protein changes induced by combined stresses, in leaves, of tolerant and susceptible genotypes. Moreover, some physiological and biochemical parameters were assessed, to understand the physiological status of tolerant and susceptible genotypes under combined stresses. Read More

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http://dx.doi.org/10.1139/bcb-2018-0338DOI Listing
February 2019

MiR-204 inhibits hepatocellular cancer drug resistance and metastasis through targeting NUAK1.

Biochem Cell Biol 2019 Feb 26. Epub 2019 Feb 26.

Changxing County Hospital, Changxing, China ;

Liver cancer is a leading cause of cancer-related deaths globally. Tumor response rate of liver cancer patients towards systemic chemotherapy is low and chemoresistance can easily develop. Identifying novel molecules that can repress drug resistance and metastasis of liver cancer will facilitate the development of new therapeutic strategies. Read More

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http://dx.doi.org/10.1139/bcb-2018-0354DOI Listing
February 2019
1 Read

Vitamin A Status Affects Weight Gain and Hepatic Glucose Metabolism in Rats Fed a High-Fat Diet.

Biochem Cell Biol 2019 Feb 25. Epub 2019 Feb 25.

The University of Tennessee at Knoxville, Nutrition , Room 229, Jessie Harris Building , 1215 West Cumberland Avenue , Knoxville, Tennessee, United States , 37996 ;

Whether vitamin A (VA) has a role in the development of metabolic abnormalities associated with intake of a high-fat diet (HFD) is unclear. Sprague-Dawley rats after weaning were fed an isocaloric VA sufficient HFD (VAS-HFD) or a VA deficient HFD (VAD-HFD) for eight weeks. Body mass, food intake, liver and adipose tissue mass, and the hepatic expression levels of key proteins for metabolism were determined. Read More

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http://dx.doi.org/10.1139/bcb-2018-0284DOI Listing
February 2019

LncRNA-ANRIL inhibits cell senescence of vascular smooth muscle cells by regulating miR-181a/Sirt1.

Biochem Cell Biol 2019 Feb 21. Epub 2019 Feb 21.

the Second Xiangya Hospital of Central South University, Department of Geriatrics , No.139, Middle Renmin Road, Furong District , Changsha , China , 410011 ;

Background: Cardiovascular disease is one of the major threats to human life and health, and vascular aging is an important cause of its occurrence. Antisense non-coding RNA in the INK4 locus (ANRIL) is a kind of long non-coding RNAs (lncRNAs) which plays important roles in cell senescence. However, the role and mechanism of ANRIL in senescence of vascular smooth muscle cells (VSMCs) are unclear. Read More

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http://dx.doi.org/10.1139/bcb-2018-0126DOI Listing
February 2019

Alanyl-glutamine ameliorates lipopolysaccharide-induced inflammation and barrier function injury in bovine jejunum epithelial cells.

Biochem Cell Biol 2019 Feb 16. Epub 2019 Feb 16.

Shandong Academy of Agricultural Sciences, 74641, Jinan, China ;

The aim of this study was to investigate the effects of Alanyl-glutamine (Ala-Gln) on the regulation of lipopolysaccharide (LPS)-induced inflammation and barrier function in bovine jejunum epithelial cells (BJECs). BJECs were exposed (or not) to 1 μg/mL LPS for 24 h to generate a pro-inflammatory model. The cells were then treated with different concentrations of Ala-Gln (0. Read More

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http://dx.doi.org/10.1139/bcb-2018-0320DOI Listing
February 2019
2 Reads

Molecular control of protein synthesis, glucose metabolism, and apoptosis in the brain of hibernating thirteen-lined ground squirrels.

Biochem Cell Biol 2019 Feb 14. Epub 2019 Feb 14.

Carleton University, 6339, Biology , 1125 Colonel By Drive , Ottawa, Ontario, Canada , K1S 5B6 ;

Thirteen-lined ground squirrels (Ictidomys tridecemlineatus) are excellent models for studying acute brain ischemia since they show high resistance to reductions in blood flow and oxygen delivery without evidence of neurological damage. In the present study, we analyzed the insulin signaling pathway and regulation of mitochondrial substrate oxidation in three regions of ground squirrel brain (forebrain, cerebellum, and brainstem), comparing summer, late torpor, and interbout arousal conditions. We found select decreases in phospho-Akt in the cerebellum during torpor as compared to summer animals, as well as select increases in the forebrain during interbout arousal, suggesting that Akt may influence either metabolism or cytoprotective pathways. Read More

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http://dx.doi.org/10.1139/bcb-2018-0256DOI Listing
February 2019
2.152 Impact Factor

Cellular roles of the human Obg-like ATPase 1 (hOLA1) and its YchF homologs .

Biochem Cell Biol 2019 Feb 11:1-11. Epub 2019 Feb 11.

a Alberta RNA Research and Training Institute (ARRTI), University of Lethbridge, 4401 University Drive W, Lethbridge, AB T1K 3M4, Canada.

P-loop NTPases comprise one of the major superfamilies of nucleotide binding proteins, which mediate a variety of cellular processes, such as mRNA translation, signal transduction, cell motility, and growth regulation. In this review, we discuss the structure and function of two members of the ancient Obg-related family of P-loop GTPases: human Obg-like ATPase 1 (hOLA1), and its bacterial/plant homolog, YchF. After a brief discussion of nucleotide binding proteins in general and the classification of the Obg-related family in particular, we discuss the sequence and structural features of YchF and hOLA1. Read More

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http://dx.doi.org/10.1139/bcb-2018-0353DOI Listing
February 2019

Involvement of M1 and M3 receptors in isolated pancreatic islets function during weight cycling in ovariectomized rats.

Biochem Cell Biol 2019 Feb 1. Epub 2019 Feb 1.

Graduate Program in Biomedical Sciences, Centro Universitário Hermínio Ometto, FHO/UNIARARAS , Av. Maximiliano Barutto nº 500, Jardim Universitário , Araras, Brazil , 13607-339 ;

We investigate structural and functional adaptations of the pancreas during weight cycling in animals submitted to hypoestrogenism. Female Wistar groups: ShamAL (AL: ad libitum), OVXAL (ovariectomized), and OVXcycle (dietary restriction with weight cycling). The ShamAL and OVXAL received ad libitum commercial feed, while the OVXcycle received 21 days of ad libitum commercial feed and 21 days of caloric restriction with 40% of the amount of feed consumed by the OVXAL. Read More

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http://dx.doi.org/10.1139/bcb-2018-0306DOI Listing
February 2019

SAV1, regulated by microRNA-21, suppresses tumor growth in colorectal cancer.

Biochem Cell Biol 2019 04 25;97(2):91-99. Epub 2019 Jan 25.

b Department of Henan Key Laboratory of Digestive Organ Transplantation, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China.

This study investigated the role and action of the Salvador 1 protein (SAV1, also called WW45) in colorectal cancer (CRC). For this, CRC SW480 and HCT116 cells were infected with lentiviruses of SAV1 overexpression vector (lenti-SAV1) and SAV1 short hairpin RNA (sh-SAV1) to overexpress and silence SAV1 respectively, or transfected with microRNA-21 (miR-21) mimic to overexpress miR-21. Relative mRNA levels of SAV1 and relative miR-21 levels in CRC tissues or cells were detected. Read More

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http://www.nrcresearchpress.com/doi/10.1139/bcb-2018-0034
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http://dx.doi.org/10.1139/bcb-2018-0034DOI Listing
April 2019
10 Reads

The long non-coding RNA HOTTIP promotes breast cancer cell migration, invasion, and epithelial-mesenchymal transition via Wnt/β-catenin pathway.

Biochem Cell Biol 2019 Jan 24. Epub 2019 Jan 24.

Shengjing Hospital of China Medical University, 85024, Department of Breast Surgery, Shenyang, Liaoning, China ;

Long non-coding RNA HOTTIP (HOXA transcript at the distal tip) has been reported to participate in proliferation of various cancer cells recently, yet its role in migration/invasion and EMT (epithelial-mesenchymal transition) of breast cancer cells and the potential mechanisms remain unknown. Breast cancer cell lines MDA-MB-231 and MDA-MB-468 were transfected with shRNA (short hairpin RNA) that specifically targeting HOTTIP. We observed a remarkable decrease in migration and invasion in two breast cancer cell lines after HOTTIP knockdown by sh-HOTTIP. Read More

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http://www.nrcresearchpress.com/doi/10.1139/bcb-2018-0313
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http://dx.doi.org/10.1139/bcb-2018-0313DOI Listing
January 2019
9 Reads

ARS2 is required for retinal progenitor cell S-phase progression and Müller glial cell fate specification .

Biochem Cell Biol 2019 Jan 23:1-11. Epub 2019 Jan 23.

a Department of Biochemistry and Microbiology, University of Victoria, Victoria, BC V8W 2Y2, Canada.

During a developmental period that extends postnatally in the mouse, proliferating multipotent retinal progenitor cells produce one of 7 major cell types (rod, cone, bipolar, horizontal, amacrine, ganglion, and Müller glial cells) as they exit the cell cycle in consecutive waves. Cell production in the retina is tightly regulated by intrinsic, extrinsic, spatial, and temporal cues, and is coupled to the timing of cell cycle exit. Arsenic-resistance protein 2 (ARS2, also known as SRRT) is a component of the nuclear cap-binding complex involved in RNA Polymerase II transcription, and is required for cell cycle progression. Read More

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http://www.nrcresearchpress.com/doi/10.1139/bcb-2018-0250
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http://dx.doi.org/10.1139/bcb-2018-0250DOI Listing
January 2019
6 Reads

Knockdown of RNF2 enhances radiosensitivity of lung squamous cell carcinoma cells.

Biochem Cell Biol 2019 Jan 23. Epub 2019 Jan 23.

The Fourth Hospital of Hebei Medical University, Department of Radiotherapy , 12 Jiankang Road , Shijiazhuang, China , 050011 ;

A previous study has reported that RING finger protein 2 (RNF2) knockdown increases radiosensitivity of esophageal cancer cells both in vitro and in vivo. However, the effect of RNF2 knockdown on radiosensitivity in squamous cell carcinoma (SqCC) remains unknown. NCI-H226 and SK-MES-1 cells were exposed to X-ray irradiation and then RNF2 levels were determined. Read More

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http://www.nrcresearchpress.com/doi/10.1139/bcb-2018-0252
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http://dx.doi.org/10.1139/bcb-2018-0252DOI Listing
January 2019
8 Reads

Protein kinase A-mediated novel Serine 584 phosphorylation of HDAC4.

Biochem Cell Biol 2019 Jan 19. Epub 2019 Jan 19.

University of Hyderabad School of Life Sciences, 98773, Hyderabad, Telangana, India ;

Given the well-established diversified histone deacetylase 4 (HDAC4) signaling pathways and the regulation of HDAC4 by several post-translational modifications (PTMs) including phosphorylation, sumoylation, ubiquitination, an unbiased and detailed analysis of HDAC4 PTMs is needed. In this study, we used matrix-assisted laser desorption/ionization time of flight (MALDI-TOF/TOF) to describe phosphorylation at serine 584 (Ser584) along with already known dual phosphorylation at serines 265 and 266 (Ser265/266), regulating together HDAC4 activity. Overexpression of site-specific HDAC4 mutants (S584A, S265/266A) in HEK 293T cells followed by HDAC activity assays revealed the mutants to be less active than the wild-type protein. Read More

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http://dx.doi.org/10.1139/bcb-2018-0208DOI Listing
January 2019
3 Reads

MicroRNA-134 Deactivates Hepatic Stellate Cells by Targeting TGF-β Activated Kinase 1-Binding Protein 1.

Biochem Cell Biol 2019 Jan 15. Epub 2019 Jan 15.

Shanghai Changzheng Hospital, Second Military Medical University, Shanghai, China ;

Aberrant expression of microRNAs is associated with liver fibrogenesis. We previously found that microRNA-134 (miR-134) expression was reduced in fibrosis-based hepatocarcinogenesis induced by diethylinitrosamine. Herein we investigate the role and mechanisms of miR-134 in hepatic fibrosis. Read More

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http://www.nrcresearchpress.com/doi/10.1139/bcb-2018-0211
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http://dx.doi.org/10.1139/bcb-2018-0211DOI Listing
January 2019
9 Reads

Enhanced susceptibility to apoptosis and growth arrest of human breast carcinoma cells treated by silica nanoparticles loaded with monohydroxy flavone compounds.

Biochem Cell Biol 2019 Jan 14. Epub 2019 Jan 14.

Department of Chemistry, Faculty of Science, Assiut University, Assiut 71516, Egypt, Assiut, Egypt ;

The treatment of drug-resistant cancer is a clinical challenge, hence screening for novel anticancer drugs is critically important. In the present study we investigated the anti-tumor potential of three plant-derived flavone compounds 3-hydroxy flavone (3-HF), 6-hydroxy flavone (6-HF) and 7-hydroxy flavone (7-HF) either alone or combined with silica nanoparticles (3-HF+NP, 6-HF+NP and 7-HF+NP) on the human breast carcinoma cell lines MDA-MB-231 and MCF-7, as well as on non-tumorigenic normal breast epithelial cells (MCF-10). The IC50 values of these flavone compounds loaded with NP (flavones+NP) in these cell lines were determined to be 1. Read More

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http://dx.doi.org/10.1139/bcb-2018-0133DOI Listing
January 2019
2 Reads
2.152 Impact Factor

Fisetin, a potential caloric restriction mimetic, attenuates senescence biomarkers in rat erythrocytes.

Biochem Cell Biol 2019 Jan 9. Epub 2019 Jan 9.

University of Allahabad, Biochemistry, Allahabad, Uttar Pradesh, India ;

An imbalanced redox status is a hallmark of the aging process. Caloric restriction mimetics (CRMs) are compounds that produce caloric restriction benefits at molecular, cellular and physiological levels translating into health-promoting effects. Fisetin is the least explored CRM and its role in modulation of oxidative stress during aging is not clearly known. Read More

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http://www.nrcresearchpress.com/doi/10.1139/bcb-2018-0159
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http://dx.doi.org/10.1139/bcb-2018-0159DOI Listing
January 2019
11 Reads

FKBP25 participates in DNA double-strand break repair .

Biochem Cell Biol 2019 Jan 8:1-8. Epub 2019 Jan 8.

a The Department of Biochemistry & Microbiology, University of Victoria, Victoria, BC V8W 3P6, Canada.

FK506-binding proteins (FKBPs) alter the conformation of proteins via cis-trans isomerization of prolyl-peptide bonds. While this activity can be demonstrated in vitro, the intractability of detecting prolyl isomerization events in cells has limited our understanding of the biological processes regulated by FKBPs. Here we report that FKBP25 is an active participant in the repair of DNA double-strand breaks (DSBs). Read More

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http://www.nrcresearchpress.com/doi/10.1139/bcb-2018-0328
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http://dx.doi.org/10.1139/bcb-2018-0328DOI Listing
January 2019
2 Reads

Knockdown of the Small Heat Shock Protein, p26 by RNA Interference Modifies the Diapause Proteome of Artemia franciscana.

Biochem Cell Biol 2019 Jan 8. Epub 2019 Jan 8.

Dalhousie University , Department of Biology , Halifax, Nova Scotia, Canada , B3H 4J1 ;

Embryos of the crustacean, Artemia franciscana may arrest as gastrulae, forming cysts that enter diapause, a state of reduced metabolism and enhanced stress tolerance. Diapausing cysts survive physiological stresses for years, due in part to molecular chaperones. p26, a small heat shock protein, is an abundant diapause-specific molecular chaperone in cysts and it affects embryo development and stress tolerance. Read More

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http://dx.doi.org/10.1139/bcb-2018-0231DOI Listing
January 2019

Cytoskeletal control of nuclear morphology and stiffness are required for OPN-induced bone marrow-derived mesenchymal stem cell migration.

Biochem Cell Biol 2019 Jan 4. Epub 2019 Jan 4.

Chongqing University, College of Bioengineering , No. 174, Shapingba Street, Shapingba District , Chongqing, China , 400030 ;

During cell migration, the movement of the nucleus must be coordinated with the cytoskeletal dynamics that influence the efficiency of cell migration. Our previous study demonstrated that osteopontin (OPN) significantly promotes the migration of bone marrow-derived mesenchymal stem cells (BMSCs). However, the mechanism of regulation of nuclear mechanics by the cytoskeleton during OPN-promoted BMSC migration remains unclear. Read More

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http://dx.doi.org/10.1139/bcb-2018-0263DOI Listing
January 2019
1 Read

Ziziphus spina-christi leaves methanolic extract alleviates diethylnitrosamine-induced hepatocellular carcinoma in rats.

Biochem Cell Biol 2019 Jan 3. Epub 2019 Jan 3.

Children's Cancer Hospital Egypt 57357, 218800, Cairo, Canada ;

The present study aimed to evaluate the antitumor activity of Ziziphus spina-christi leaves extract (ZSCL) against diethylnitrosamine (DENA)-induced hepatocarcinogenesis in rats. The phytochemical constituents, in vitro antioxidant and cytotoxic activities of ZSCL extract were investigated. Male Wistar rats were divided into 6 groups: normal control, ZSCL1-administered rats (100 mg/ Kg body weight "b. Read More

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http://dx.doi.org/10.1139/bcb-2018-0318DOI Listing
January 2019
3 Reads

Fetal alcohol spectrum disorder (FASD) affects the hippocampal levels of histone variant H2A.Z-2.

Biochem Cell Biol 2019 Jan 3. Epub 2019 Jan 3.

University of Victoria , Department of Biochemistry and Microbiology , P.O. Box 3055 , Victoria, British Columbia, Canada , V8W 3P6 ;

Fetal Alcohol Spectrum disorder (FASD) is caused by the prenatal exposure to ethanol and has been linked to neurodevelopmental impairments. Alcohol has the potential to alter some of the epigenetic components which play a critical role during development. Previous studies have provided evidence that prenatal ethanol exposure results in abnormal epigenetic patterns (i. Read More

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http://dx.doi.org/10.1139/bcb-2018-0240DOI Listing
January 2019

MicroRNA-34a attenuates VEGF-mediated retinal angiogenesis via targeting the Notch1.

Biochem Cell Biol 2018 Dec 20. Epub 2018 Dec 20.

Shengjing Hospital of China Medical University, 85024, Shenyang, Liaoning, China ;

Pathological angiogenesis in retina is main pathogenesis of ocular diseases closely associated with vision loss. Present work aimed to investigate effects of microRNA-34a (miR-34a) and its potential target Notch1 on retinal angiogenesis. For this objective, we employed oxygen-induced retinopathy (OIR) rats and human retinal microvascular endothelial cells (HRMECs) stimulated with vascular endothelial growth factors (VEGF). Read More

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http://www.nrcresearchpress.com/doi/10.1139/bcb-2018-0304
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http://dx.doi.org/10.1139/bcb-2018-0304DOI Listing
December 2018
9 Reads

GBP1 exerts inhibitory effects on acute viral myocarditis through the inhibition of inflammatory response of macrophage in mice.

Biochem Cell Biol 2018 Dec 17. Epub 2018 Dec 17.

China-Japan Union Hospital of Jilin University, 74569, Cardiology , No. 126, Xiantai Street , Changchun, China , 130031 ;

Viral myocarditis (VMC) is a condition that could potentially progress to dilated cardiomyopathy or congestive heart failure, making it the leading cause of the untimely death in young adults. Interferon-induced GBP1 encodes much GTPase induced by interferon gamma in many eukaryotic cells. However, there is little known regarding the effect of GBP1 on acute VMC (AVMC). Read More

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http://dx.doi.org/10.1139/bcb-2018-0135DOI Listing
December 2018
7 Reads

Steroid receptor coactivator-1 regulates glioma angiogenesis through polyomavirus enhancer activator 3 signaling.

Authors:
Yi Zhang Wei Shi

Biochem Cell Biol 2018 Dec 11. Epub 2018 Dec 11.

Second Affiliated Hospital of Xi'an Jiao Tong University, Department of Neurosurgery, Xi'an, Shaanxi, China ;

Steroid receptor coactivator 1 (SRC-1) is a transcriptional coactivator for steroid receptors and other transcription factors. SRC-1 has been shown to play an important role in the progression of breast cancer and prostate cancer. However, its role in glioma progression remains unknown. Read More

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http://dx.doi.org/10.1139/bcb-2018-0114DOI Listing
December 2018
2.152 Impact Factor

Carboxypeptidase E-ΔN promotes migration, invasion and epithelial-mesenchymal transition of human osteosarcoma cell lines through the Wnt/β-catenin pathway.

Biochem Cell Biol 2018 Dec 3. Epub 2018 Dec 3.

The First Affiliated hospital of China Medical University, Department of Orthopedic Surgery, Shenyang, China ;

Osteosarcoma (OS) is the most common malignant bone tumor in children and adolescents, and metastatic OS is the major cause of OS-related death. Carboxypeptidase E (CPE) is demonstrated to be highly expressed in some cancer types, and its N-terminal truncated form, CPE-ΔN, is implicated in tumor metastasis and poor prognosis. In this study, we investigated the effect of CPE-ΔN on cell migration, invasion and epithelial-mesenchymal transition (EMT) of OS cells, and illustrated the molecular mechanisms. Read More

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http://www.nrcresearchpress.com/doi/10.1139/bcb-2018-0236
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http://dx.doi.org/10.1139/bcb-2018-0236DOI Listing
December 2018
8 Reads

Vitamin E supplementation and caloric restriction promotes regulation of insulin secretion and glycemic homeostasis by different mechanisms in rats.

Biochem Cell Biol 2018 Nov 27:1-9. Epub 2018 Nov 27.

a Graduate Program in Biomedical Sciences, Centro Universitário Hermínio Ometto, UNIARARAS, Araras, São Paulo, Brazil.

Vitamin E and caloric restriction have antioxidant effects in mammals. The aim of this study was to evaluate effects of vitamin E supplementation and caloric restriction upon insulin secretion and glucose homeostasis in rats. Male Wistar rats were distributed among the following groups: C, control group fed ad libitum; R, food quantity reduction of 40%; CV, control group supplemented with vitamin E [30 mg·kg·day]; and RV, food-restricted group supplemented with vitamin E. Read More

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http://dx.doi.org/10.1139/bcb-2018-0066DOI Listing
November 2018

miR-3666 inhibits lung cancer cell proliferation, migration and invasion by targeting BPTF.

Biochem Cell Biol 2018 Nov 27. Epub 2018 Nov 27.

Women's Hospital of Nanjing Medical University, Nanjing Maternity and Child Health Care Hospital, Nanjing, China ;

Our previous study suggested that BPTF overexpression was observed in lung adenocarcinoma, and closely associated with advanced clinical stage, more metastatic lymph nodes, present distant metastasis, low histological grade and poor prognosis. Down-regulation of BPTF inhibited lung adenocarcinoma cells proliferation and promoted lung adenocarcinoma cells apoptosis. The purpose of this study is to identify valuable microRNAs (miRNAs), which target BPTF to modulate lung adenocarcinoma cells proliferation. Read More

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http://www.nrcresearchpress.com/doi/10.1139/bcb-2018-0301
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http://dx.doi.org/10.1139/bcb-2018-0301DOI Listing
November 2018
19 Reads

Ginsenoside metabolite compound K induces apoptosis and autophagy in non-small cell lung cancer cells via AMPK/mTOR and JNK pathways.

Biochem Cell Biol 2018 Nov 26. Epub 2018 Nov 26.

The General Hospital of First Automotive Works, The Fourth Hospital of Jilin University, Changchun, China ;

Compound K (C-K, 20-O-(β-D-glucopyranosyl)-20(S)-protopanaxadiol), as a metabolite of ginsenoside, has been verified to have antitumor effects in various cancers, including non-small cell lung cancer (NSCLC). However, the detailed mechanisms of C-K in NSCLC remain largely unknown. In this study, we aimed to evaluate the effect of C-K on apoptosis and autophagy in NSCLC cells as well as its related mechanisms. Read More

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http://dx.doi.org/10.1139/bcb-2018-0226DOI Listing
November 2018
2 Reads

Shikonin exerts antitumor activity by causing mitochondrial dysfunction in hepatocellular carcinoma through PKM2-AMPK-PGC1α signaling pathway.

Biochem Cell Biol 2018 Nov 26. Epub 2018 Nov 26.

Nanchang Univ , 999 Xuefu road, Honggutan New District, Nanchang, Jiangxi, China , Nanchang, China , 330031 ;

Shikonin, a naphthoquinone derivative isolated from the root of Lithospermum erythrorhizon exhibited broad-spectrum antitumor activity via different molecular mechanisms. In the present study, we investigated the effect of shikonin on the mitochondrial dysfunction in hepatocellular carcinoma (HCC). Our results showed that shikonin inhibited the proliferation, migration, and invasion of HCCLM3 cells, and meanwhile, promoted cell apoptosis in a dose-dependent manner. Read More

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http://dx.doi.org/10.1139/bcb-2018-0310DOI Listing
November 2018
2 Reads
2.152 Impact Factor

Corrigendum: Ameliorating effects of Sporidiobolus pararoseus extract on dyslipidemia in mice with high fat diet induced obesity.

Biochem Cell Biol 2018 12 23;96(6):847. Epub 2018 Nov 23.

b School of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue Wuxi, Jiangsu Province, 214122, P. R. China.

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http://dx.doi.org/10.1139/bcb-2018-0359DOI Listing
December 2018
2.152 Impact Factor

Role of N-glycosylation in the expression of human SLC26A2 and A3 anion transport membrane glycoproteins .

Biochem Cell Biol 2018 Nov 21:1-17. Epub 2018 Nov 21.

a Department of Biochemistry, University of Toronto, Toronto, ON M5S 1A8, Canada.

The human solute carrier 26 (SLC26) gene family of anion transporters consists of 10 members (SLC26A1-A11, A10 being a pseudogene) that encode membrane glycoproteins with 14 transmembrane segments and a C-terminal cytoplasmic sulfate transporter anti-sigma antagonist domain. Thus far, mutations in eight members of the SLC26 family (A1-A6, A8, and A9) have been linked to diseases in humans. Our goal is to characterize the role of N-glycosylation and the effect of mutations in SLC26A2 and A3 proteins on their functional expression in transfected HEK-293 cells. Read More

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http://dx.doi.org/10.1139/bcb-2018-0139DOI Listing
November 2018
12 Reads

Quantum Mechanical Tunnelling Through the Catalytic Effects of A2451 Ribosomal Residue During a Stepwise Peptide Bond Formation.

Biochem Cell Biol 2018 Nov 16. Epub 2018 Nov 16.

Universiti Malaya, Department of Physics, Kuala Lumpur, Malaysia ;

The search for the mechanism of ribosomal peptide bond formation is still ongoing. Even though the actual mechanism of peptide bod formation is still a dilemma, the dominance of proton transfer in this reaction is known for certain. Therefore, it is vital to take the quantum mechanical effects on proton transfer reaction into consideration, the effects of which were neglected in all previous studies. Read More

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http://dx.doi.org/10.1139/bcb-2018-0220DOI Listing
November 2018
11 Reads

Glutathione: subcellular distribution and membrane transport .

Biochem Cell Biol 2018 Nov 14:1-20. Epub 2018 Nov 14.

a Cellular Biochemistry, University of Kaiserslautern, 67663 Kaiserslautern, Germany.

Glutathione (γ-l-glutamyl-l-cysteinylglycine) is a small tripeptide found at millimolar concentrations in nearly all eukaryotes as well as many prokaryotic cells. Glutathione synthesis is restricted to the cytosol in animals and fungi and to the cytosol and plastids in plants. Nonetheless, glutathione is found in virtually all subcellular compartments. Read More

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http://www.nrcresearchpress.com/doi/10.1139/bcb-2018-0189
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http://dx.doi.org/10.1139/bcb-2018-0189DOI Listing
November 2018
14 Reads

LRF acts as an activator and repressor of the human β-like globin gene transcription in a developmental stage dependent manner.

Biochem Cell Biol 2018 Nov 14. Epub 2018 Nov 14.

Pusan National University, 34996, Kumjeong-ku, Korea (the Republic of) ;

LRF, a hematopoietic transcription factor, has been suggested to repress fetal γ-globin genes in the human adult stage β-globin locus. Here, to study the role of LRF in the fetal stage β-globin locus we knocked out its expression in erythroid K562 cells, in which the γ-globin genes are mainly transcribed. The γ-globin transcription was reduced in LRF KO cells, and transcription factor binding to the β-globin locus control region hypersensitive sites (LCR HSs) and active histone organization in the LCR HSs were disrupted by the depletion of LRF. Read More

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http://dx.doi.org/10.1139/bcb-2018-0303DOI Listing
November 2018
7 Reads

How Alcohol Drinking Affects our Genes: an Epigenetic Point of View.

Biochem Cell Biol 2018 Nov 9. Epub 2018 Nov 9.

Centro Riferimento Alcologico Regione Lazio, Sapienza University of Rome, Italy, Rome, Italy ;

This work highlights recent studies in epigenetic mechanisms that play a role in alcoholism, a complex multifactorial disorder. A large body of evidence has shown that alcohol can modify gene expression through epigenetic processes, namely DNA methylation and nucleosomal remodeling via histone modifications. In that vein, chronic ethanol exposure modifies DNA and histone methylation, histone acetylation, and microRNA expression. Read More

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http://www.nrcresearchpress.com/doi/10.1139/bcb-2018-0248
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http://dx.doi.org/10.1139/bcb-2018-0248DOI Listing
November 2018
16 Reads

Lysophosphatidic acid represses autophagy in prostate carcinoma cells.

Biochem Cell Biol 2018 Nov 7. Epub 2018 Nov 7.

Akdeniz University, Faculty of Medicine, Department of Medical Biochemistry, Antalya, Turkey ;

Lysophosphatidic acid (LPA) is a small signaling phospholipid that mediates diverse functions including cell proliferation, migration and survival by engaging LPA-agonized G-protein coupled receptors. Autophagy is a survival mechanism in response to nutrient depletion or organellar damage that encloses idle or damaged organelles within autophagosomes that are then delivered to lysosomes for degradation. However, the relationship between LPA and autophagy is largely unknown. Read More

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http://www.nrcresearchpress.com/doi/10.1139/bcb-2018-0164
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http://dx.doi.org/10.1139/bcb-2018-0164DOI Listing
November 2018
11 Reads