1,103,058 results match your criteria Biochemistry & physiology[Journal]


Lipid remodelling in hepatocyte proliferation and hepatocellular carcinoma.

Hepatology 2020 May 27. Epub 2020 May 27.

Department of Biochemistry and Cambridge Systems Biology Centre, University of Cambridge, Cambridge, United Kingdom.

Background & Aims: Hepatocytes undergo profound metabolic rewiring when primed to proliferate during compensatory regeneration and in hepatocellular carcinoma (HCC). However, the metabolic control of these processes is not fully understood. In order to capture the metabolic signature of proliferating hepatocytes, we applied state-of-the-art systems biology approaches to models of liver regeneration, pharmacologically- and genetically-activated cell proliferation, and HCC. Read More

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http://dx.doi.org/10.1002/hep.31391DOI Listing

Brazilian berry extract (Myrciaria jaboticaba): A promising therapy to minimize prostatic inflammation and oxidative stress.

Prostate 2020 May 27. Epub 2020 May 27.

Department of Structural and Functional Biology, Institute of Biology, University of Campinas, São Paulo, Brazil.

Background: Brazilian berry is a fruit popularly known as "Jaboticaba," rich in bioactive compounds with antioxidant and anti-inflammatory properties. Senescence and overweight are increasing worldwide and are considered risk factors to prostatic pathogenesis mainly due to oxidative and inflammatory processes induction. Thus, this study aimed to evaluate the effect of two increasing doses of the patented jaboticaba peel extract (PJE) on oxidative-stress and inflammation in the prostate of aging or high-fat-fed aging mice. Read More

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http://dx.doi.org/10.1002/pros.24017DOI Listing

Synergism between albumin, bicarbonate and cAMP upregulation for cholesterol efflux from ram sperm.

Reproduction 2020 May 1. Epub 2020 May 1.

T Leahy, School of Life and Environmental Sciences, Faculty of Science, The University of Sydney, Sydney, Australia.

Compared to other mammalian species, ram spermatozoa are difficult to capacitate in vitro. Dibutyryl cAMP (db-cAMP) and the phosphodiesterase (PDE) inhibitors, caffeine and theophylline (cAMP up-regulators), must be added to traditional capacitation media (containing bicarbonate, calcium and bovine serum albumin; BSA), to elicit a capacitation response. In this exploratory study, we assessed whether bicarbonate was still required for ram spermatozoa if cAMP is upregulated by the addition of db-cAMP and PDE inhibitors, and what role BSA plays in cholesterol efflux under these conditions. Read More

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http://dx.doi.org/10.1530/REP-19-0430DOI Listing

SPOP promotes ubiquitination and degradation of LATS1 to enhance kidney cancer progression.

EBioMedicine 2020 May 3;56:102795. Epub 2020 May 3.

Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325027, China. Electronic address:

Background: Emerging evidence has demonstrated that SPOP functions as an oncoprotein in kidney cancer to promote tumorigenesis by ubiquitination-mediated degradation of multiple regulators of cellular proliferation and apoptosis. However, the detailed molecular mechanism underlying the oncogenic role of SPOP in kidney tumorigenesis remains elusive.

Methods: Multiple approaches such as Co-IP, Transfection, RT-PCR, Western blotting, and animal studies were utilized to explore the role of SPOP in kidney cancer. Read More

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http://dx.doi.org/10.1016/j.ebiom.2020.102795DOI Listing

Serum and placental periostin levels in women with early pregnancy loss.

J Reprod Immunol 2020 May 15;140:103138. Epub 2020 May 15.

Department of Gynecology and Obstetrics, Baskent University, Konya, Turkey.

Objectives: Periostin is secreted from the placenta in the embryonic period and it is emphasized that it may be involved in endometrial implantation. In this study, we aimed to investigate periostin serum levels and placental tissue expression in first trimester pregnancy losses.

Study Design: In this prospective case-control study, 30 patients who underwent dilatation and curettage with first trimester spontaneous abortion (<10 weeks of gestation) were included in the study group and 30 patients who had voluntary pregnancy termination (<10 gestational weeks) were included in the control group. Read More

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http://dx.doi.org/10.1016/j.jri.2020.103138DOI Listing

In Vivo Generation of Post-infarct Human Cardiac Muscle by Laminin-Promoted Cardiovascular Progenitors.

Cell Rep 2020 May;31(8):107714

Cardiovascular & Metabolic Disorders Program, Duke-NUS Medical School, National University of Singapore, Singapore 169857, Singapore; Department of Medical Biochemistry and Biophysics, Karolinska Institute, 171 77 Stockholm, Sweden. Electronic address:

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http://dx.doi.org/10.1016/j.celrep.2020.107714DOI Listing

MKRN2 Physically Interacts with GLE1 to Regulate mRNA Export and Zebrafish Retinal Development.

Cell Rep 2020 May;31(8):107693

Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada; Center for Network Systems Biology, Boston University, Boston, MA, USA. Electronic address:

The mammalian mRNA nuclear export process is thought to terminate at the cytoplasmic face of the nuclear pore complex through ribonucleoprotein remodeling. We conduct a stringent affinity-purification mass-spectrometry-based screen of the physical interactions of human RNA-binding E3 ubiquitin ligases. The resulting protein-interaction network reveals interactions between the RNA-binding E3 ubiquitin ligase MKRN2 and GLE1, a DEAD-box helicase activator implicated in mRNA export termination. Read More

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http://dx.doi.org/10.1016/j.celrep.2020.107693DOI Listing

Novel Forms of Immunomodulation for Cancer Therapy.

Trends Cancer 2020 Jun 31;6(6):518-532. Epub 2020 Mar 31.

Apoptosis, Immunity, and Cancer Group, Department of Biochemistry and Molecular and Cell Biology, University of Zaragoza, and Aragon Health Research Institute (IIS-Aragon), Zaragoza 50009, Spain.

In recent years immunotherapy has provided new hope for cancer patients. However, some patients eventually relapse. Immunological responses are thought to underlie the long-term effects of conventional or targeted therapies. Read More

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http://dx.doi.org/10.1016/j.trecan.2020.02.015DOI Listing

Metagenomics reveals impact of geography and acute diarrheal disease on the Central Indian human gut microbiome.

Gut Microbes 2020 May 27:1-24. Epub 2020 May 27.

Biochemistry Research Centre, Central India Institute of Medical Sciences , Nagpur, India.

Background: The Central Indian gut microbiome remains grossly understudied. Herein, we sought to investigate the burden of antimicrobial resistance and diarrheal diseases, particularly , in rural-agricultural and urban populations in Central India, where there is widespread unregulated antibiotic use. We utilized shotgun metagenomics to comprehensively characterize the bacterial and viral fractions of the gut microbiome and their encoded functions in 105 participants. Read More

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http://dx.doi.org/10.1080/19490976.2020.1752605DOI Listing

Translating 'protein foods' from the new Canada's Food Guide to consumers: Knowledge gaps and recommendations.

Appl Physiol Nutr Metab 2020 May 27. Epub 2020 May 27.

University of Manitoba , Room 209 Human Ecology , Department of Human Nutritional Sciences , Winnipeg, Manitoba, Canada , R3T 2N2 ;

The revised version of Canada's Food Guide, released in January 2019, issued new guidance by combining meat and alternatives with milk and alternatives into a single group called 'protein foods' and emphasized selecting plant-based foods from this category more often. Though the changes represent a simple depiction of a healthy plate, the new Food Guide has opened knowledge gaps about 'protein foods' and exposed new concerns about the interpretation and implementation of the Food Guide among vulnerable groups, particularly children and the elderly. To address key knowledge and research gaps, nutrition leaders need to reach a consensus on key messages to best inform the development of tools and resources to support practitioners in translating messages to consumers, including foodservice standards. Read More

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http://dx.doi.org/10.1139/apnm-2020-0192DOI Listing

Feline Interleukin-31 shares overlapping epitopes with Oncostatin M Receptor and IL-31RA.

Biochemistry 2020 May 27. Epub 2020 May 27.

Interleukin-31 (IL-31) is a major protein involved in severe inflammatory skin disorders. Its signaling pathway is mediated through two type I cytokine receptors, IL-31RA (also known as gp130-like receptor) and oncostatin M receptor (OSMR). Understanding molecular details in these interactions would be helpful for developing antagonist anti-IL-31 monoclonal antibodies (mAbs) as potential therapies. Read More

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http://dx.doi.org/10.1021/acs.biochem.0c00176DOI Listing

α-triazolylboronic acids: a promising scaffold for effective inhibitors of KPCs.

ChemMedChem 2020 May 27. Epub 2020 May 27.

Case Western Reserve University, Department of Medicine, Pharmacology, Biochemistry and Molecular Biology and Microbiology, UNITED STATES.

Boronic Acids are known reversible covalent inhibitors of serine β-lactamases. The selectivity and high potency of specific boronates bearing an amide side chain mimicking the β-lactam's amide side chain have been advanced in several studies. Herein, we describe a new class of boronic acids where the amide group is replaced by a bioisostere triazole. Read More

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http://dx.doi.org/10.1002/cmdc.202000126DOI Listing

Separating Growth from Nucleation for Facile Control over the Size and Shape of Palladium Nanocrystals.

Chemistry 2020 May 27. Epub 2020 May 27.

Georgia Institute of Technology, College of Sciences, North Avenue, MoSE 3100J, 30332, Atlanta, UNITED STATES.

In order to maximize the performance of nanocrystals in a specific application, it is necessary to control both their size and shape. Here we report a one-pot protocol that allows us to separate growth from nucleation for achieving better controls over the size and shape of Pd nanocrystals. The two processes are temporally separated from each other, although the synthesis is carried out in the same reaction container. Read More

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http://dx.doi.org/10.1002/chem.202001626DOI Listing

The role of nitric oxide in glutaric acid-induced convulsive behavior in pup rats.

Eur J Neurosci 2020 May 27. Epub 2020 May 27.

Laboratório de Síntese, Reatividade e Avaliação Farmacológica e Toxicológica de Organocalcogênios, Departamento de Bioquímica e Biologia Molecular, Centro de Ciências Naturais e Exatas, Federal University of Santa Maria (UFSM), Santa Maria, RS, 97105-900, Brazil.

Glutaric acidemia type I (GA-I) is a cerebral organic disorder characterized by the accumulation of glutaric acid (GA) and seizures. Since seizures are precipitated in children with GA-I and the mechanisms underlying this disorder are not well established, we decided to investigate the role of nitric oxide (NO) in GA-induced convulsive behavior in pup rats. Pup male Wistar rats (18-day-old) were anesthetized and placed in stereotaxic apparatus for cannula insertion into the striatum for injection of GA. Read More

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http://dx.doi.org/10.1111/ejn.14840DOI Listing

Inhibition of the NAD salvage pathway in schistosomes impairs metabolism, reproduction, and parasite survival.

PLoS Pathog 2020 May 27;16(5):e1008539. Epub 2020 May 27.

Department of Microbiology, The University of Alabama at Birmingham, Birmingham, Alabama, United States of America.

NAD, a key co-enzyme required for cell metabolism, is synthesized via two pathways in most organisms. Since schistosomes apparently lack enzymes required for de novo NAD biosynthesis, we evaluated whether these parasites, which infect >200 million people worldwide, maintain NAD homeostasis via the NAD salvage biosynthetic pathway. We found that intracellular NAD levels decline in schistosomes treated with drugs that block production of nicotinamide or nicotinamide mononucleotide-known NAD precursors in the non-deamidating salvage pathway. Read More

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http://dx.doi.org/10.1371/journal.ppat.1008539DOI Listing

Topoisomerase II-binding protein 1 promotes the progression of prostate cancer via ATR-CHK1 signaling pathway.

Aging (Albany NY) 2020 May 27;12. Epub 2020 May 27.

Department of Urology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, China.

DNA damage response (DDR) plays an important role in the progression of cancers, including prostate cancer (PCa). Topoisomerase II-binding protein 1 (TopBP1) is an essential promotor of ATR-mediated DDR. Herein, we investigated the association between TopBP1 and PCa and determined its effect on the progression of PCa. Read More

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http://dx.doi.org/10.18632/aging.103260DOI Listing

Nek8445, a protein kinase required for microtubule regulation and cytokinesis in .

Mol Biol Cell 2020 May 27:mbcE19070406. Epub 2020 May 27.

Department of Biology, University of Washington, Seattle, WA 98195.

has 198 Nek kinases whereas humans have only 11. has a complex microtubule cytoskeleton that includes eight flagella and several unique microtubule arrays that are utilized for parasite attachment and facilitation of rapid mitosis and cytokinesis. The need to regulate these structures may explain the parallel expansion of the number of Nek family kinases. Read More

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http://dx.doi.org/10.1091/mbc.E19-07-0406DOI Listing

Crystal Structure and Electronic Properties of New Compound ZrPtSe.

Inorg Chem 2020 May 27. Epub 2020 May 27.

Department of Physics, The University of Texas at Dallas, Richardson, Texas 75080, United States.

A new ternary nonstoichiometric ZrPtSe has been discovered as a part of effort to dope Zr into the layered transitional metal chalcogenide PtSe. With a new structure type (68), it is the first Pt-based ternary chalcogenide with group 4 elements (Ti, Zr, and Hf). The crystal structure adopts the orthorhombic space group with lattice parameters of = 15. Read More

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http://dx.doi.org/10.1021/acs.inorgchem.0c00522DOI Listing

Inter- and Intra-Molecular Interactions of The Arabidopsis Plasma Membrane Proton Pump Revealed Using a Mass Spectrometric-Cleavable Crosslinker.

Biochemistry 2020 May 27. Epub 2020 May 27.

In plants and fungi, the plasma membrane proton pump (H+-ATPase) establishes an electrochemical gradient across the plasma membrane which serves as the driving force for the secondary transport of ions and nutrients across the cell membrane. This is an essential enzyme that functions in many important processes including stomatal movement, cell elongation, and cellular responses to stimuli from hormones, light, and other environmental conditions. Therefore, understanding how the activity of the H+-ATPase is regulated is important to understand how plants adapt to different growth conditions. Read More

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http://dx.doi.org/10.1021/acs.biochem.0c00268DOI Listing

Epigenetic Responses to Acute Resistance Exercise in Trained vs. Sedentary Men.

J Strength Cond Res 2020 Jun;34(6):1574-1580

Department of Kinesiology, Biochemistry and Molecular Exercise Physiology Laboratory, Center for Sport Performance, California State University, Fullerton, California.

Bagley, JR, Burghardt, KJ, McManus, R, Howlett, B, Costa, PB, Coburn, JW, Arevalo, JA, Malek, MH, and Galpin, AJ. Epigenetic responses to acute resistance exercise in trained vs. sedentary men. Read More

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http://dx.doi.org/10.1519/JSC.0000000000003185DOI Listing

HRP2-DPF3a-BAF complex coordinates histone modification and chromatin remodeling to regulate myogenic gene transcription.

Nucleic Acids Res 2020 May 27. Epub 2020 May 27.

2011 Collaborative Innovation Center of Tianjin for Medical Epigenetics, Tianjin Key Laboratory of Medical Epigenetics, Key Laboratory of Immune Microenvironment and Disease (Ministry of Education), Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Tianjin Medical University, Tianjin 300070, China.

Functional crosstalk between histone modifications and chromatin remodeling has emerged as a key regulatory mode of transcriptional control during cell fate decisions, but the underlying mechanisms are not fully understood. Here we discover an HRP2-DPF3a-BAF epigenetic pathway that coordinates methylated histone H3 lysine 36 (H3K36me) and ATP-dependent chromatin remodeling to regulate chromatin dynamics and gene transcription during myogenic differentiation. Using siRNA screening targeting epigenetic modifiers, we identify hepatoma-derived growth factor-related protein 2 (HRP2) as a key regulator of myogenesis. Read More

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http://dx.doi.org/10.1093/nar/gkaa441DOI Listing

The hyperthermophilic partners Nanoarchaeum and Ignicoccus stabilize their tRNA T-loops via different but structurally equivalent modifications.

Nucleic Acids Res 2020 May 27. Epub 2020 May 27.

Department of Biochemistry & Molecular Biology, University of Southern Denmark, Campusvej 55, DK-5230 Odense M, Denmark.

The universal L-shaped tertiary structure of tRNAs is maintained with the help of nucleotide modifications within the D- and T-loops, and these modifications are most extensive within hyperthermophilic species. The obligate-commensal Nanoarchaeum equitans and its phylogenetically-distinct host Ignicoccus hospitalis grow physically coupled under identical hyperthermic conditions. We report here two fundamentally different routes by which these archaea modify the key conserved nucleotide U54 within their tRNA T-loops. Read More

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http://dx.doi.org/10.1093/nar/gkaa411DOI Listing

Relationships between maternal antibody responses and early childhood infection with KSHV.

J Infect Dis 2020 May 27. Epub 2020 May 27.

Nebraska Center for Virology, Lusaka, Zambia.

While mother-to-child transmission is believed to play in important role in early childhood infection with Kaposi's sarcoma-associated herpesvirus (KSHV), the maternal immune response remains largely uncharacterized. This study aimed to characterize the longitudinal humoral response to KSHV in a cohort of HIV-infected Zambian mothers without KS and identify potential factors that may influence transmission. In total 86/124 (69. Read More

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http://dx.doi.org/10.1093/infdis/jiaa288DOI Listing

Restriction endonucleases that cleave RNA/DNA heteroduplexes bind dsDNA in A-like conformation.

Nucleic Acids Res 2020 May 27. Epub 2020 May 27.

International Institute of Molecular and Cell Biology, Trojdena 4, 02-109 Warsaw, Poland.

Restriction endonucleases naturally target DNA duplexes. Systematic screening has identified a small minority of these enzymes that can also cleave RNA/DNA heteroduplexes and that may therefore be useful as tools for RNA biochemistry. We have chosen AvaII (G↓GWCC, where W stands for A or T) as a representative of this group of restriction endonucleases for detailed characterization. Read More

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http://dx.doi.org/10.1093/nar/gkaa403DOI Listing

The ABC transporter G subfamily in Arabidopsis thaliana.

J Exp Bot 2020 May 27. Epub 2020 May 27.

Institute of Biochemistry and Cluster of Excellence on Plant Sciences CEPLAS, Heinrich Heine University Düsseldorf, Düsseldorf, Germany.

ABC transporters are ubiquitously present in all kingdoms and mediate the transport of a large spectrum of structurally different compounds. Plants possess high numbers of ABC transporters in relation to other eukaryotes, especially the ABCG subfamily is enlarged. Earlier studies demonstrate that ABCG transporters are involved in important processes influencing plant fitness. Read More

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http://dx.doi.org/10.1093/jxb/eraa260DOI Listing

Distinct classes of multi-subunit heterogeneity: analysis using Fourier Transform methods and native mass spectrometry.

Analyst 2020 May 27. Epub 2020 May 27.

Department of Chemistry and Biochemistry, 1253 University of Oregon, Eugene, OR 97403-1253, USA. and Materials Science Institute, 1252 University of Oregon, Eugene, OR 97403-1252, USA.

Native electrospray mass spectrometry is a powerful method for determining the native stoichiometry of many polydisperse multi-subunit biological complexes, including multi-subunit protein complexes and lipid-bound transmembrane proteins. However, when polydispersity results from incorporation of multiple copies of two or more different subunits, it can be difficult to analyze subunit stoichiometry using conventional mass spectrometry analysis methods, especially when m/z distributions for different charge states overlap in the mass spectrum. It was recently demonstrated by Marty and co-workers (K. Read More

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http://dx.doi.org/10.1039/d0an00726aDOI Listing

Molecular, serological, and biochemical diagnosis and monitoring of COVID-19: IFCC taskforce evaluation of the latest evidence.

Clin Chem Lab Med 2020 May 27. Epub 2020 May 27.

IFCC Taskforce on COVID-19, International Federation of Clinical Chemistry, Milan, Italy.

The global coronavirus disease 2019 (COVID-19) has presented major challenges for clinical laboratories, from initial diagnosis to patient monitoring and treatment. Initial response to this pandemic involved the development, production, and distribution of diagnostic molecular assays at an unprecedented rate, leading to minimal validation requirements and concerns regarding their diagnostic accuracy in clinical settings. In addition to molecular testing, serological assays to detect antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are now becoming available from numerous diagnostic manufacturers. Read More

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http://dx.doi.org/10.1515/cclm-2020-0722DOI Listing

Epigenetic inheritance of circadian period in clonal cells.

Elife 2020 May 27;9. Epub 2020 May 27.

Department of Neuroscience, Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, United States.

Circadian oscillations are generated via transcriptional-translational negative feedback loops. However, individual cells from fibroblast cell lines have heterogeneous rhythms, oscillating independently and with different period lengths. Here we showed that heterogeneity in circadian period is heritable and used a multi-omics approach to investigate underlying mechanisms. Read More

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http://dx.doi.org/10.7554/eLife.54186DOI Listing

The architecture of EMC reveals a path for membrane protein insertion.

Elife 2020 May 27;9. Epub 2020 May 27.

Cell Biology Division, MRC Laboratory of Molecular Biology, Cambridge, United Kingdom.

Approximately 25% of eukaryotic genes code for integral membrane proteins that are assembled at the endoplasmic reticulum. An abundant and widely conserved multi-protein complex termed EMC has been implicated in membrane protein biogenesis, but its mechanism of action is poorly understood. Here, we define the composition and architecture of human EMC using biochemical assays, crystallography of individual subunits, site-specific photocrosslinking, and cryo-EM reconstruction. Read More

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http://dx.doi.org/10.7554/eLife.57887DOI Listing

Structural insights into sodium transport by the oxaloacetate decarboxylase sodium pump.

Elife 2020 May 27;9. Epub 2020 May 27.

Department of Biochemistry and Molecular Biology, Tianjin Medical University, Tianjin, China.

The oxaloacetate decarboxylase sodium pump (OAD) is a unique primary-active transporter that utilizes the free energy derived from oxaloacetate decarboxylation for sodium transport across the cell membrane. It is composed of 3 subunits: the a subunit catalyzes carboxyl-transfer from oxaloacetate to biotin, the membrane integrated β subunit catalyzes the subsequent carboxyl-biotin decarboxylation and the coupled sodium transport, the γ subunit interacts with the a and β subunits and stabilizes the OAD complex. We present here structure of the OAD βγ sub-complex. Read More

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http://dx.doi.org/10.7554/eLife.53853DOI Listing

Metal-Sulfur Compounds in N Reduction and Nitrogenase-Related Chemistry.

Chem Rev 2020 May 27. Epub 2020 May 27.

Department of Chemsitry, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8602, Japan.

Transition metal-sulfur (M-S) compounds are an indispensable means for biological systems to convert N into NH (biological N fixation), and these may have emerged by chemical evolution from a prebiotic N fixation system. With a main focus on synthetic species, this article provides a comprehensive review of the chemistry of M-S compounds related to the conversion of N and the structures/functions of the nitrogenase cofactors. Three classes of M-S compounds are highlighted here: multinuclear M-S clusters structurally or functionally relevant to the nitrogenase cofactors, mono- and dinuclear transition metal complexes supported by sulfur-containing ligands in N and NH ( = 2, 4) chemistry, and metal sulfide-based solid materials employed in the reduction of N. Read More

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http://dx.doi.org/10.1021/acs.chemrev.9b00544DOI Listing

Improved Flow Cytometric Light Scatter Detection of Submicron-Sized Particles by Reduction of Optical Background Signals.

Cytometry A 2020 May 27. Epub 2020 May 27.

Department of Biochemistry and Cell Biology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.

Flow cytometry allows multiparameter analysis on a single-cell basis and is currently the method of choice to rapidly assess heterogeneity of cell populations in suspension. With the research field of extracellular vesicles (EV) rapidly expanding, there is an increased demand to address heterogeneity of EV populations in biological samples. Although flow cytometry would be the ideal technique to do so, the available instruments are in general not equipped to optimally detect the dim light scatter signals generated by submicron-sized particles like EV. Read More

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http://dx.doi.org/10.1002/cyto.a.24036DOI Listing

Electrocatalytic reactivity of imine/oxime-type cobalt complex for direct perfluoroalkylation of indole and aniline derivatives.

Dalton Trans 2020 May 27. Epub 2020 May 27.

Department of Chemistry and Biochemistry, Graduate School of Engineering, Kyushu University 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan. and Center for Molecular Systems (CMS), Kyushu University 744 Motooka, Nishi-ku, Fukuoka 819-0395, Japan.

Imine/Oxime-type cobalt complexes, regarded as simple vitamin B12 model complexes, were utilized as catalysts for direct C-H perfluoroalkylations of indole and aniline derivatives with nonafluorobutyl iodide (n-C4F9I) as the readily available perfluoroalkyl source. The synthetic approach described herein was performed under mild reaction conditions driven by controlled-potential electrolysis at -0.8 V vs. Read More

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http://dx.doi.org/10.1039/d0dt01377cDOI Listing

Pt-Co truncated octahedral nanocrystals: a class of highly active and durable catalysts toward oxygen reduction.

Nanoscale 2020 May 27. Epub 2020 May 27.

The Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia 30332, USA. and School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia 30332, USA.

We report a facile and scalable synthesis of Pt-Co truncated octahedral nanocrystals (TONs) by employing Pt(acac)2 and Co(acac)2 as precursors, together with CO molecules and Mn atoms derived from the decomposition of Mn2(CO)10 as a reductant and a {111} facet-directing agent, respectively. Both the composition and yield of the Pt-Co TONs could be varied through the introduction of CHCl3. When tested at 80 °C using membrane electrode assembly (MEA), the 4 nm Pt2. Read More

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http://dx.doi.org/10.1039/d0nr02904aDOI Listing

Enantioselective synthesis enabled by visible light photocatalysis.

Authors:
Bor-Cherng Hong

Org Biomol Chem 2020 May 27. Epub 2020 May 27.

Department of Chemistry and Biochemistry, National Chung Cheng University, Chia-Yi, 621, Taiwan, Republic of China.

Enantioselective photoreaction has been a synthetic challenge for decades. With the continuous development of modern visible light photocatalysis and asymmetric catalysis, remarkable advances have been achieved through the synergistic action of these catalytic reactions, allowing the construction of various enantiomerically enriched molecules that were once inaccessible using photocatalytic reactions. This review presents some of the contemporary developments in enantioselective visible-light photocatalysis reactions, covering the period from 2008 to March 2020, with the contents classified by catalysis type. Read More

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http://dx.doi.org/10.1039/d0ob00759eDOI Listing

Diving into the world of marine 2,11-cyclized cembranoids: a summary of new compounds and their biological activities.

Nat Prod Rep 2020 May 27. Epub 2020 May 27.

State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Zhangjiang Hi-Tech Park, Shanghai 201203, China. and University of Chinese Academy of Sciences, No. 19A Yuquan Road, Beijing 100049, China.

Covering: 2010 to 2020The class of 2,11-cyclized cembranoids is particularly widespread in marine Octocorallia, especially Alcyonacea and Gorgonacea, and has been extensively studied. Following a previous review published in 2010, the accumulated knowledge from the past decade will be presented here. All 2,11-cyclized cembranoids share a bicyclo[8. Read More

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http://dx.doi.org/10.1039/d0np00016gDOI Listing

N-Ligated nickel(ii) diketonate complexes: synthesis, characterization and evaluation of O reactivity.

Dalton Trans 2020 May 27. Epub 2020 May 27.

Department of Chemistry & Biochemistry, Utah State University, 0300 Old Main Hill, Logan, UT 84322-0300, USA.

Interest in O2-dependent aliphatic carbon-carbon (C-C) bond cleavage reactions of first row divalent metal diketonate complexes stems from the desire to further understand the reaction pathways of enzymes such as DKE1 and to extract information to develop applications in organic synthesis. A recent report of O2-dependent aliphatic C-C bond cleavage at ambient temperature in Ni(ii) diketonate complexes supported by a tridentate nitrogen donor ligand [(MBBP)Ni(PhC(O)CHC(O)Ph)]Cl (7-Cl; MBBP = 2,6-bis(1-methylbenzimidazol-2-yl)pyridine) in the presence of NEt3 spurred our interest in further examining the chemistry of such complexes. A series of new TERPY-ligated Ni(ii) diketonate complexes of the general formula [(TERPY)Ni(R2-1,3-diketonate)]ClO4 (1: R = CH3; 2: R = C(CH3)3; 3: R = Ph) was prepared under air and characterized using single crystal X-ray crystallography, elemental analysis, 1H NMR, ESI-MS, FTIR, and UV-vis. Read More

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http://dx.doi.org/10.1039/d0dt01338bDOI Listing

Self-assembly of chimeric peptides toward molecularly defined hexamers with controlled multivalent ligand presentation.

Chem Commun (Camb) 2020 May 27. Epub 2020 May 27.

Department of Chemistry and Biochemistry, The University of Texas at Arlington, Arlington, TX 76019, USA.

This work demonstrates a self-assembling peptide strategy to form finite, molecularly defined trigonal bipyramidal-like hexamers which offer control over multivalent ligand display for enhanced tumor targeting. Read More

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http://dx.doi.org/10.1039/d0cc02066dDOI Listing

Cortical bone maturation in mice requires SOCS3 suppression of gp130/STAT3 signalling in osteocytes.

Elife 2020 May 27;9. Epub 2020 May 27.

St. Vincent's Institute of Medical Research, Fitzroy, Australia.

Bone strength is determined by its dense cortical shell, generated by unknown mechanisms. Here we use the mouse, with delayed cortical bone consolidation, to characterise cortical maturation and identify control signals. We show that cortical maturation requires a reduction in cortical porosity, and a transition from low to high density bone, which continues even after cortical shape is established. Read More

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http://dx.doi.org/10.7554/eLife.56666DOI Listing

Characterization of the kinetic cycle of an ABC transporter by single-molecule and cryo-EM analyses.

Elife 2020 May 27;9. Epub 2020 May 27.

Laboratory of Nanoscale Biophysics and Biochemistry, The Rockefeller University, New York, United States.

ATP-binding cassette (ABC) transporters are molecular pumps ubiquitous across all kingdoms of life. While their structures have been widely reported, the kinetics governing their transport cycles remain largely unexplored. Multidrug resistance protein 1 (MRP1) is an ABC exporter that extrudes a variety of chemotherapeutic agents and native substrates. Read More

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http://dx.doi.org/10.7554/eLife.56451DOI Listing

Barrita Virus, a Novel Virus of the Patois Serogroup (Genus ; Family Peribunyaviridae).

Am J Trop Med Hyg 2020 May 26. Epub 2020 May 26.

Center for Tropical Diseases, Galveston, Texas.

During ecological investigations for arboviruses conducted in coastal Chiapas, Mexico, in 2007, isolate MP1078 was obtained from a pool of mosquitoes. Based on antigenic characterization, this isolate was classified as a strain of Patois virus (PATV) ( genus, Peribunyaviridae family). Recently, we conducted nearly complete genome sequencing of this isolate to gain further insight into its genetic relationship with other members of the Patois serogroup. Read More

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http://dx.doi.org/10.4269/ajtmh.19-0906DOI Listing

LAT1 (SLC7A5) Overexpression in Negative Her2 Group of Breast Cancer: A Potential Therapy Target.

Asian Pac J Cancer Prev 2020 May 1;21(5):1453-1458. Epub 2020 May 1.

Department of Pathology, Jordan University of Science and Technology, Irbid, Jordan.

Objective: HER2 negative carcinomas of the breast pose a challenge for treatment due to redundancies in potential drug targets and poor patient outcomes. Our aim was to investigate the role of L-type amino acid transporter - LAT1 as a potential prognosticator and a drug target.

Methods: In this retrospective work, we have studied the expression of LAT1 in 145 breast cancer tissues via immunohistochemistry. Read More

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http://dx.doi.org/10.31557/APJCP.2020.21.5.1453DOI Listing

Prevalence and Antibiotic Resistance Patterns of Helicobacter pylori Infection in Koh Kong, Cambodia.

Asian Pac J Cancer Prev 2020 May 1;21(5):1409-1413. Epub 2020 May 1.

Department of Environmental and Preventive Medicine, Oita University Faculty of Medicine, Yufu, Japan.

Background: Gastric cancer, which is the leading cause of cancer mortality in Cambodia, can be prevented by Helicobacter pylori (H. pylori) eradication. There is limited data about H. Read More

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http://dx.doi.org/10.31557/APJCP.2020.21.5.1409DOI Listing

Evaluation of the Biological Efficiency of Silver Nanoparticles Biosynthesized Using Croton tiglium L. Seeds Extract against Azoxymethane Induced Colon Cancer in Rats.

Asian Pac J Cancer Prev 2020 May 1;21(5):1369-1389. Epub 2020 May 1.

Animal Reproduction and Artificial Insemination Department, Veterinary Division, National Research Centre, Dokki, Giza, Egypt .

Background: Colorectal cancer (CRC) is considered as the most common type of gastrointestinal cancers. Chemotherapy became limited due to the adverse side effects. Therefore, the most effective Croton tiglium extract was selected to be incorporated by silver nanoparticles (Ag-NPs) then evaluated against colon cancer induced by azoxymethane (AOM) in rats. Read More

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http://dx.doi.org/10.31557/APJCP.2020.21.5.1369DOI Listing

Ethanol Extract of Hizikia fusiforme Induces Apoptosis in B16F10 Mouse Melanoma Cells through ROS-Dependent Inhibition of the PI3K/Akt Signaling Pathway.

Asian Pac J Cancer Prev 2020 May 1;21(5):1275-1282. Epub 2020 May 1.

Anti-Aging Research Center, Dong-eui University, Busan 47340, Republic of Korea.

Background: Previous studies have reported that Hizikia fusiforme, an edible brown seaweed, has diverse health-promoting effects; however, evidence for its anti-cancer potential is still lacking. In this study, we examined the effect of ethanol extract of H. fusiforme (EHF) on the proliferation of B16F10 mouse melanoma cells. Read More

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http://dx.doi.org/10.31557/APJCP.2020.21.5.1275DOI Listing

Reversal Effect of Dihydromyricetin on Multiple Drug Resistance in SGC7901/5-FU Cells.

Asian Pac J Cancer Prev 2020 May 1;21(5):1269-1274. Epub 2020 May 1.

Department of Biochemistry, Wannan Medical College, Wuhu, Anhui, P.R.China.

Background: One of the most common treatment for gastric cancer is chemotherapy, however, multiple drug resistance (MDR) induce the therapeutic effect which result in the failure of anticancer therapy. Dihydromyricetin (DMY) was reported to have antitumor activities on various human cancer cells in vitro, our previous studies demonstrated that DMY combined with mitomycin has inhibitory effect on proliferation of gastric carcinoma cells. However, the underlying role of DMY reversing the MDR of gastric carcinoma is poor understood. Read More

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http://dx.doi.org/10.31557/APJCP.2020.21.5.1269DOI Listing

Association of EGLN2 rs10680577 Polymorphism with the Risk and Clinicopathological Features of Patients with Prostate Cancer.

Asian Pac J Cancer Prev 2020 May 1;21(5):1221-1226. Epub 2020 May 1.

Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.

Several studies have evaluated the association between EGLN2 4-bp insertion/deletion (ins/del) polymorphism (rs10680577) and many cancers. However, up to date, no study has inspected the impact of rs10680577 polymorphism on prostate cancer (PCa) risk. This case-control study was achieved on 170 pathologically confirmed PCa patients and 196 cancer free men to inspect whether rs10680577 variant is related to the risk and clinicopathological features of patients with PCa. Read More

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http://dx.doi.org/10.31557/APJCP.2020.21.5.1221DOI Listing

On the Formation of the Popcorn Flavorant 2,3-Butanedione (CH3COCOCH3) in Acetaldehyde-Containing Interstellar ices.

Chemphyschem 2020 May 26. Epub 2020 May 26.

University of Hawaii at Manoa, 2545 McCarthy Mall, 96822, Honolulu, UNITED STATES.

Acetaldehyde (CH 3 CHO) is ubiquitous throughout the interstellar medium and has been observed in cold molecular clouds, star forming regions, and in meteorites such as Murchison. As the simplest methyl-bearing aldehyde, acetaldehyde constitutes a critical precursor to prebiotic molecules such as the sugar deoxyribose and amino acids via the Strecker synthesis. In this study, we reveal the first laboratory detection of 2,3-butanedione (diacetyl, CH 3 COCOCH 3 )-a butter and popcorn flavorant-synthesized within acetaldehyde-based interstellar analog ices exposed to ionizing radiation at 5 K. Read More

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http://dx.doi.org/10.1002/cphc.202000116DOI Listing

Induction of γ-aminobutyric acid plays a positive role to Arabidopsis resistance against Pseudomonas syringae.

J Integr Plant Biol 2020 May 26. Epub 2020 May 26.

State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, 310058, China.

GABA (γ-aminobutyric acid) is an important metabolite that functions in plant growth, development, and stress responses. However, its role in plant defense and how it is regulated are largely unknown. Here, we report a detailed analysis of GABA induction during the resistance response to Pseudomonas syringae in Arabidopsis thaliana. Read More

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http://dx.doi.org/10.1111/jipb.12974DOI Listing

Spurious, marked leukocytosis in 2 cats with Heinz body hemolytic anemia.

Vet Clin Pathol 2020 May 26. Epub 2020 May 26.

Department of Veterinary Clinical Sciences, College of Veterinary Medicine, University of Minnesota, St. Paul, MN, USA.

Two domestic shorthair cats were presented with anorexia and dehydration following ingestion of caramelized onions. Shared key findings from a CBC (ADVIA 2120), serum biochemistry, and urinalysis included a spurious, marked leukocytosis with discordant basophil (BASO) channel and peroxidase channel WBC counts, normal manual leukocyte counts, mild, non-regenerative anemia with discrepancies between automated and manual reticulocyte counts, an abundance of large Heinz bodies (HBs), and highly irregular scattergrams. Case 1 also demonstrated a markedly elevated mean corpuscular hemoglobin concentration (MCHC) and discrepancies between RBC hemoglobin indices. Read More

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http://dx.doi.org/10.1111/vcp.12860DOI Listing
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