Search our Database of Scientific Publications and Authors

I’m looking for a

    82510 results match your criteria Biochemical and biophysical research communications[Journal]

    1 OF 1651

    Relaxin alleviates TGFβ1-induced cardiac fibrosis via inhibition of Stat3-dependent autophagy.
    Biochem Biophys Res Commun 2017 Sep 20. Epub 2017 Sep 20.
    Department of Cardiology, The First Affiliated Hospital, Harbin Medical University, Harbin 150001, China; Key Laboratory of Cardiac Diseases and Heart Failure, Harbin Medical University, Harbin 150001, China; Institute of Metabolic Disease, Heilongjiang Academy of Medical Science, Harbin 150081, China. Electronic address:
    Cardiac fibrosis is a pathological feature common to a variety of heart diseases such as myocardial infarction, arrhythmias, cardiomyopathies and heart failure. Emerging data has indicted that autophagy is involved in fibrotic synthesis. Relaxin as a pleiotropic hormone can attenuate cardiac fibrosis and hypertrophy, however the exact molecular mechanism remains largely unknown. Read More

    Molecular characterization of mitochondrial Zucchini and its relation to nuage-piRNA pathway components in Bombyx mori ovary-derived BmN4 cells.
    Biochem Biophys Res Commun 2017 Sep 20. Epub 2017 Sep 20.
    Laboratory of Insect Genome Science, Department of Bioresource Science, Graduate School of Bioresource and Bioenvironmental Sciences, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan. Electronic address:
    Piwi-interacting RNAs (piRNAs) are a class of small non-coding RNAs that associate with PIWI subfamily proteins, which play an important role in transposon silencing in animal germ cell. The piRNAs biogenesis is divided into two major pathways: primary and secondary, and both pathways are independent of double-stranded RNA-processing enzyme Dicer, which processes the single-stranded RNA transcripts in microRNA (miRNA) and siRNA (small interfering RNA) pathway. Primary piRNAs are processed from long non-coding RNA precursors transcribed from piRNA clusters. Read More

    SDF-1 improves wound healing ability of glucocorticoid-treated adipose tissue-derived mesenchymal stem cells.
    Biochem Biophys Res Commun 2017 Sep 20. Epub 2017 Sep 20.
    Laboratory of Regenerative Medicine and Stem Cell Biology, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Japan. Electronic address:
    Glucocorticoids cause the delayed wound healing by suppressing inflammation that is required for wound healing process. Adipose tissue-derived mesenchymal stem cells (AT-MSCs) play an important role for wound healing by their cytokine productions including stromal derived factor 1 (SDF-1). However, it has not been clear how glucocorticoids affect the wound healing ability of AT-MSCs. Read More

    Fibulin-5 promotes airway smooth muscle cell proliferation and migration via modulating Hippo-YAP/TAZ pathway.
    Biochem Biophys Res Commun 2017 Sep 20. Epub 2017 Sep 20.
    Department of Emergency, Xi'an Children's Hospital, Xi'an 710003, Shaanxi, China. Electronic address:
    Asthma is a common chronic disease mainly occurs from childhood. Increased airway smooth muscle mass is involved in the pathogenesis of asthma. Fibulin-5 was upregulated in the lung tissues of patients with COPD and idiopathic pulmonary fibrosis. Read More

    High glucose derived endothelial microparticles increase active caspase-3 and reduce microRNA-Let-7a expression in endothelial cells.
    Biochem Biophys Res Commun 2017 Sep 20. Epub 2017 Sep 20.
    Integrative Vascular Biology Laboratory, Department of Integrative Physiology, University of Colorado, Boulder, CO 80309, USA. Electronic address:
    The experimental aim of this study was to determine the effects of high glucose-induced endothelial microparticles (EMPs) on endothelial cell susceptibility to apoptosis. Human umbilical vein endothelial cells (HUVECs) were cultured (3rd passage) and plated in 6-well plates at a density of 5.0 × 10(5) cells/condition. Read More

    Arabidopsis PECP1 and PS2 are phosphate starvation-inducible phosphocholine phosphatases.
    Biochem Biophys Res Commun 2017 Sep 20. Epub 2017 Sep 20.
    Institute of Plant and Microbial Biology, Academia Sinica, 128 sec.2, Academia Rd., Nankang, Taipei 11529, Taiwan. Electronic address:
    Phosphate-starved plants reduce phosphatidylcholine content presumably to provide an internal phosphate source while replacing membrane phospholipids by galactolipids, a process termed membrane lipid remodeling. However, whether the metabolic fate of released phosphocholine is a phosphate source remains elusive because primary phosphocholine phosphatases in vivo are unknown in seed plants. Here, we show that PECP1 and PS2 are the primary phosphocholine phosphatases in Arabidopsis and function redundantly under phosphate starvation. Read More

    Leptin positively regulates MUC5AC production and secretion induced by interleukin-13 in human bronchial epithelial cells.
    Biochem Biophys Res Commun 2017 Sep 20. Epub 2017 Sep 20.
    Department of Pulmonary Medicine, Qingdao Municipal Hospital, School of Medicine, Qingdao University, Qingdao 266011, China. Electronic address:
    Mucus hypersecretion and plugging of lower respiratory tract airways due to mucus plugs have long been recognized as the leading cause of the morbidity and mortality in asthma. MUC5AC protein is a major component of airway mucus. Here, we showed that interleukin (IL)-13 induced MUC5AC production and secretion, and leptin expression in the human bronchial epithelial cell line-16 (HBE16) cells in a concentration-dependent manner. Read More

    Gut microbial metabolite TMAO contributes to renal dysfunction in a mouse model of diet-induced obesity.
    Biochem Biophys Res Commun 2017 Sep 20. Epub 2017 Sep 20.
    Department of Nephrology, Shengjing Hospital, China Medical University, China.
    Emerging evidence shows that obesity induces renal injury and is an independent risk factor for the development of chronic kidney disease (CKD), even without diabetes or hyperglycemia. Although multiple metabolic factors have been suggested to account for obesity-associated renal injury, the precious underlying mechanisms are not completely understood. Recent study shows that increased trimethylamine N-Oxide (TMAO), a gut microbiota-generated metabolite, directly contributes to renal interstitial fibrosis and dysfunction. Read More

    Microgravity influences maintenance of the human muscle stem/progenitor cell pool.
    Biochem Biophys Res Commun 2017 Sep 20. Epub 2017 Sep 20.
    Department of Surgery and Clinical Science, Yamaguchi University Graduate School of Medicine, Yamaguchi, Japan.
    Microgravity induces skeletal muscle atrophy; however, the underlying mechanism is not clarified. In particular, the influence of microgravity on human skeletal muscle stem/progenitor cells (SMPCs) is not well understood. In this study, we used induced pluripotent stem cell-derived human SMPCs to investigate the effect of microgravity on maintenance of the stem/progenitor cell pool. Read More

    ARID1A suppresses malignant transformation of human pancreatic cells via mediating senescence-associated miR-503/CDKN2A regulatory axis.
    Biochem Biophys Res Commun 2017 Sep 20. Epub 2017 Sep 20.
    Medical Laboratory, Taizhou Central Hospital (Taizhou University Hospital), Taizhou, Zhejiang, China. Electronic address:
    ARID1A as a subunit of SWI/SNF chromatin complexes is frequently mutated in human pancreatic cancer, however its exact role in pancreatic tumorigenesis remain unclear. In this study, we investigated the effects of ARID1A loss on human pancreatic epithelial cell lines HPNE, BxPC-3 with KRAS mutant (KRAS(G12D)) expression. We found that ARID1A knockdown promoted cell proliferation and colony formation in cooperation with active mutant KRAS(G12D). Read More

    Transplantation of dedifferentiation fat cells promotes intervertebral disc regeneration in a rat intervertebral disc degeneration model.
    Biochem Biophys Res Commun 2017 Sep 20. Epub 2017 Sep 20.
    Department of Orthopedic Surgery, Nihon University School of Medicine, Tokyo 173-8610, Japan. Electronic address:
    Our group has reported that mature adipocyte-derived dedifferentiated fat (DFAT) cells show multilineage differentiation potential similar to that observed in mesenchymal stem cells. In the present study, we examined whether DFAT cell transplantation could contribute to intervertebral disc regeneration using a rat intervertebral disc degeneration (IDD) model. The IDD was created in Sprague-Dawley rats by puncturing at level of caudal intervertebral disc under fluoroscopy. Read More

    Carbon monoxide regulates glycolysis-dependent NLRP3 inflammasome activation in macrophages.
    Biochem Biophys Res Commun 2017 Sep 20. Epub 2017 Sep 20.
    Soonchunhyang Institute of Medi-bio Science (SIMS), Soonchunhyang University, Cheonan-si, Chungcheongnam-do, Republic of Korea. Electronic address:
    Low dose of carbon monoxide (CO) has anti-inflammatory role through various signaling pathways. Cellular metabolism has been implicated in the activation of inflammation in immune cells. However, the mechanisms by which CO-dependent metabolic regulation affect the immune response remain unclear. Read More

    Neuroligin-induced presynaptic differentiation through SLM2-mediated splicing modifications of neurexin in cerebellar cultures.
    Biochem Biophys Res Commun 2017 Sep 19. Epub 2017 Sep 19.
    Institute of Innovative Science and Technology, Medical Division, Tokai University, Isehara City, Kanagawa, Japan. Electronic address:
    Neurexins (NRXs) and neuroligins (NLs) play important roles in synapse specification. The alternatively spliced segment 4 (AS4) of NRX genes (Nrxn) is a critical element in selective trans-synaptic interactions. However, the role of splicing of NRXs and NLs in synapse specification is not fully understood. Read More

    Inorganic phosphate-induced impairment of osteoclast cell-cell fusion by the inhibition of AP-1-mediated DC-STAMP expression.
    Biochem Biophys Res Commun 2017 Sep 19. Epub 2017 Sep 19.
    Department of Clinical Pharmacology, Faculty of Medical Sciences, Kyushu University, Fukuoka, Japan.
    Chronic kidney disease (CKD) causes hyperphosphatemia and secondary hyperparathyroidism, leading to several disorders of bone metabolism. Although high concentrations of extracellular inorganic phosphate (Pi) inhibit osteoclastogenesis, the molecular mechanism of this effect has not been fully understood. In the present study, therefore, we examined the effect of Pi on the differentiation of the osteoclast precursor RAW-D cells. Read More

    Identification of transketolase as a target of PARIS in substantia nigra.
    Biochem Biophys Res Commun 2017 Sep 19. Epub 2017 Sep 19.
    Division of Pharmacology, Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine, Samsung Biomedical Research Institute, Suwon 440-746, South Korea; Samsung Medical Center (SMC), Sungkyunkwan University School of Medicine, Samsung Biomedical Research Institute, Suwon, South Korea. Electronic address:
    Recently, PARIS (ZNF746) is introduced as authentic substrate of parkin and transcriptionally represses PGC-1α by binding to insulin responsive sequences (IRSs) in the promoter of PGC-1α. The overexpression of PARIS selectively leads to the loss of dopaminergic neurons (DN) and mitochondrial abnormalities in the substantia nigra (SN) of Parkinson's disease (PD) models. To identify PARIS target molecules altered in SN region-specific manner, LC-MS/MS-based quantitative proteomic analysis is employed to investigate proteomic alteration in the cortex, striatum, and SN of AAV-PARIS injected mice. Read More

    Stress stimuli induce cancer-stemness gene expression via Sp1 activation leading to therapeutic resistance in glioblastoma.
    Biochem Biophys Res Commun 2017 Sep 19. Epub 2017 Sep 19.
    Center for Neurotrauma and Neuroregeneration, Taipei Medical University, Taiwan; The Ph.D. Program for Neural Regenerative Medicine, Taipei Medical University, Taiwan. Electronic address:
    It has been suggested that stress stimuli from the microenvironment maintain a subset of tumor cells with stem-like properties, including drug resistance. Here, we investigate whether Sp1, a stress-responsive factor, regulates stemness gene expression and if its inhibition sensitizes cancer cells to chemotherapy. Hydrogen peroxide- and serum deprivation-induced stresses were performed in glioblastoma (GBM) cells and patient-derived cells, and the effect of the Sp1 inhibitor mithramycin A (MA) on these stress-induced stem cells and temozolomide (TMZ)-resistant cells was evaluated. Read More

    Hyperosmolarity evokes histamine release from ileum mucosa by stimulating a cholinergic pathway.
    Biochem Biophys Res Commun 2017 Sep 19. Epub 2017 Sep 19.
    Department of Thoracic Surgery, Shandong Cancer Hospital and Institute, Shandong Academy of Medical Sciences, Jinan, China. Electronic address:
    Changes in extracellular osmolarity lead to alteration in cellular volume. In the study, we examined the effects of hyperosmolarity on short-circuit currents (Isc) in the rat ileum using the Ussing chamber technique. Mucosal exposure to 20 mM glucose evoked a decrease of ISC in the rat ileum, which was antagonized by the stretch-activated channel blocker GdCl3, TTX and atropine, respectively. Read More

    Human alpha A-crystallin missing N-terminal domain poorly complexes with filensin and phakinin.
    Biochem Biophys Res Commun 2017 Sep 18. Epub 2017 Sep 18.
    Department of Optometry and Vision Science, University of Alabama at Birmingham, Birmingham, AL 35294, United States. Electronic address:
    The aim of this study was to determine relative importance of N-terminal domain and C-terminal extension of αA-crystallin during their in vitro complex formation with phakinin and filensin (the two lens-specific intermediate filament [IF] proteins). Cloned phakinin, filensin and vimentin were purified under a denaturing conditions by consecutive DEAE-cellulose-, hydroxyapatite- and Sephadex G-75-column chromatographic methods. WTαA-crystallin, αA-NT (N-terminal domain [residue number 1-63])-deleted and αA-CT (C-terminal terminal extension [residue number 140-173]-deleted), were cloned in pET 100 TOPO vector, expressed in BL-21 (DE3) cells using 1% IPTG, and purified using a Ni(2+)-affinity column. Read More

    Structural insights into the dimer-tetramer transition of FabI from Bacillus anthracis.
    Biochem Biophys Res Commun 2017 Sep 18. Epub 2017 Sep 18.
    Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul 02841, South Korea. Electronic address:
    Enoyl-ACP reductase (ENR, also known as FabI) has received considerable interest as an anti-bacterial target due to its essentiality in fatty acid synthesis. All the FabI structures reported to date, regardless of the organism, are composed of homo-tetramers, except for two structures: Bacillus cereus and Staphylococcus aureus FabI (bcFabI and saFabI, respectively), which have been reported as dimers. However, the reason for the existence of the dimeric form in these organisms and the biological meaning of dimeric and tetrameric forms of FabI are ambiguous. Read More

    A Sir2 family protein Rv1151c deacetylates HU to alter its DNA binding mode in Mycobacterium tuberculosis.
    Biochem Biophys Res Commun 2017 Sep 18. Epub 2017 Sep 18.
    Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore, Karnataka, 560064, India. Electronic address:
    Till recently, knowledge about epigenetic regulation in bacterial world confined largely to DNA methylation. Lysine acetylation/deacetylation of histones is a major contributor for chromatin dynamics in eukaryotes. However, little is known about such epigenetic changes brought about by post-translational modifications in bacteria. Read More

    The hierarchical structure of the perichromosomal layer comprises Ki67, ribosomal RNAs, and nucleolar proteins.
    Biochem Biophys Res Commun 2017 Sep 18. Epub 2017 Sep 18.
    Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tenno-dai, Tsukuba Science City, Ibaraki 305-8577, Japan; Center of Tsukuba Advanced Research Alliance (TARA), University of Tsukuba, 1-1-1 Tenno-dai, Tsukuba Science City, Ibaraki 305-8577, Japan. Electronic address:
    The perichromosomal layer (PCL) is a structure that surrounds mitotic chromosomes, found in both animal and plant cells. It comprises various proteins and RNAs, mainly derived from the nucleolus. Several functions for the PCL have been suggested; however, the mechanism of PCL organization during mitosis remains unclear. Read More

    A three-dimensional model of RNase P in the hyperthermophilic archaeon Pyrococcus horikoshii OT3.
    Biochem Biophys Res Commun 2017 Sep 18. Epub 2017 Sep 18.
    Laboratory of Structural Biology, Graduate School of Systems Life Sciences, Hakozaki 6-10-1, Fukuoka, 812-8581, Japan; Laboratory of Biochemistry, Department of Bioscience and Biotechnology, Graduate School, Faculty of Agriculture, Kyushu University, Hakozaki 6-10-1, Fukuoka, 812-8581, Japan. Electronic address:
    Ribonuclease P (RNase P) is an endoribonuclease involved in maturation of the 5'-end of tRNA. We found previously that RNase P in the hyperthermophilic archaeon Pyrococcus horikoshii OT3 consists of a catalytic RNase P RNA (PhopRNA) and five protein cofactors designated PhoPop5, PhoRpp21, PhoRpp29, PhoRpp30, and PhoRpp38. The crystal structures of the five proteins have been determined, a three-dimensional (3-D) model of PhopRNA has been constructed, and biochemical data, including protein-RNA interaction sites, have become available. Read More

    UBE2T promotes hepatocellular carcinoma cell growth via ubiquitination of p53.
    Biochem Biophys Res Commun 2017 Sep 18. Epub 2017 Sep 18.
    Department of Hepatobiliary and Pancreas Surgery, Second Clinical Medical College of Jinan University, Shenzhen People's Hospital, Shenzhen, Guangdong Province, China. Electronic address:
    Deregulation of Ubiquitin-conjugating enzyme E2T (UBE2T) contributes to the progression of human cancers. However, its clinical significance and role in hepatocellular carcinoma (HCC) remain unclear. Here, we show that UBE2T is up-regulated in HCC and exerts oncogenic activities via ubiquitination of p53. Read More

    ADAM9 is present at endothelial cell - Cell junctions and regulates monocyte - Endothelial transmigration.
    Biochem Biophys Res Commun 2017 Sep 16. Epub 2017 Sep 16.
    University of Cambridge Department of Oncology, Cancer Research UK Cambridge Institute, Li Ka-Shing Centre, Robinson Way, Cambridge, CB2 0RE, UK.
    We have found that A Disintegrin And Metalloproteinase-9 (ADAM9) localises to cell-cell junctions with VE-Cadherin in confluent endothelial monolayers. Co-cultures of cells separately transfected with ADAM9-EGFP or ADAM9-HA showed expression is required in two adjacent cells for localisation to cell-cell junctions suggesting the ADAM9 ectodomain may self-associate. A direct interaction between ADAM9 ectodomains was confirmed using recombinant proteins and an ELISA based method. Read More

    Identification of hypoxia-regulated angiogeneic genes in colorectal cancer.
    Biochem Biophys Res Commun 2017 Sep 16. Epub 2017 Sep 16.
    Department of Geriatrics, Shanghai Municipal Hospital of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200071, China. Electronic address:
    The tumour hypoxia would trigger the angiogenesis switch for survival, and increase the ability of cancer cells to invade and metastasis. However, hypoxia regulated genes that invovled in angiogenesis in colorectal cancer (CRC) have not been explored in detail. The aim of this study was to explore angiogenic genes under hypoxia condition in CRC. Read More

    Effect of sodium-glucose cotransporter 2 (SGLT2) inhibition on weight loss is partly mediated by liver-brain-adipose neurocircuitry.
    Biochem Biophys Res Commun 2017 Sep 18. Epub 2017 Sep 18.
    Nutrigenomics Research Group, Faculty of Medicine, University of Tsukuba, Ibaraki, 305-8575, Japan; Department of Internal Medicine (Endocrinology and Metabolism), Faculty of Medicine, University of Tsukuba, Ibaraki, 305-8575, Japan; Department of Internal Medicine, Graduate School of Medicine, University of Tokyo, Tokyo, 113-8655, Japan. Electronic address:
    Sodium-glucose cotransporter 2 (SGLT2) inhibitors have both anti-diabetic and anti-obesity effects. However, the precise mechanism of the anti-obesity effect remains unclear. We previously demonstrated that the glycogen depletion signal triggers lipolysis in adipose tissue via liver-brain-adipose neurocircuitry. Read More

    Idh2 deficiency accelerates renal dysfunction in aged mice.
    Biochem Biophys Res Commun 2017 Sep 18. Epub 2017 Sep 18.
    School of Life Sciences, BK21 Plus KNU Creative BioResearch Group, College of Natural Sciences, Kyungpook National University, Taegu, South Korea. Electronic address:
    The free radical or oxidative stress theory of aging postulates that senescence is due to an accumulation of cellular oxidative damage, caused largely by reactive oxygen species (ROS) that are produced as by-products of normal metabolic processes in mitochondria. The oxidative stress may arise as a result of either increased ROS production or decreased ability to detoxify ROS. The availability of the mitochondrial NADPH pool is critical for the maintenance of the mitochondrial antioxidant system. Read More

    P2Y12 antibody inhibits platelet activity and protects against thrombogenesis.
    Biochem Biophys Res Commun 2017 Sep 16. Epub 2017 Sep 16.
    Department of Pharmaceutical Sciences, School of Pharmacy, The University of Texas at El Paso, El Paso, TX, 79902, USA. Electronic address:
    Given that platelet hyperactivity is known to give rise to thrombotic disorders, new and/or novel antiplatelet therapies are constantly being developed to add to, or to complement the current arsenal of agents. To this end, adenosine diphosphate (ADP) is an important platelet activator that acts by binding to the G-protein coupled P2Y1 and P2Y12 receptors. Although the contribution of the P2Y12 receptor to the genesis of thrombosis is well established, the parenteral arsenal of drugs targeting this receptor in clinical use is limited to cangrelor. Read More

    Investigation of antidiabetic potential of Phyllanthus niruri L. using assays for α-glucosidase, muscle glucose transport, liver glucose production, and adipogenesis.
    Biochem Biophys Res Commun 2017 Sep 19. Epub 2017 Sep 19.
    Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, Universitetsparken 2, DK-2100, Copenhagen, Denmark.
    Phyllanthus niruri is used in herbal medicine for treatment of diabetes. The objective of this study was to investigate the antidiabetic potential of P. niruri, using assays for α-glucosidase, muscle glucose transport, liver glucose production and adipogenesis. Read More

    Effects of plant- and animal-based high-fat diets on lipid storage and distribution in environmental bacteria-colonized gnotobiotic mice.
    Biochem Biophys Res Commun 2017 Sep 18. Epub 2017 Sep 18.
    Graduate Institute of Veterinary Pathobiology, National Chung Hsin University, Taichung 402, Taiwan.
    Different edible oils such as lard and soybean oil have been reported to interact with the gut microbiota, affecting host lipid metabolism. However, whether bacteria derived from the environment influence host lipid metabolism remains unclear. This study aimed to clarify the roles of environmental bacteria in host lipid storage and distribution with various edible oils. Read More

    KLF15 regulates in vitro chondrogenic differentiation of human mesenchymal stem cells by targeting SOX9.
    Biochem Biophys Res Commun 2017 Sep 16. Epub 2017 Sep 16.
    Department of Orthopaedics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450003, China. Electronic address:
    Mesenchymal stem cells (MSCs) are multipotent stromal cells residing in the bone marrow. MSCs have the potential to differentiate into adipocytes, chondrocytes, and other types of cells. However, the mechanism underlying MSC differentiation is still not fully understood. Read More

    A20 inhibits both the degradation and limited processing of the NF-κB p105 precursor: A novel additional layer to its regulator role.
    Biochem Biophys Res Commun 2017 Sep 18. Epub 2017 Sep 18.
    Cancer and Vascular Biology Research Center, The Rappaport Faculty of Medicine and Research Institute, Technion - Israel Institute of Technology, P.O. Box 9649, Haifa 31096, Israel; Department of Internal Medicine B, Lady Davis/"Carmel" Medical Center, Haifa 34362, Israel. Electronic address:
    p105 plays dual roles in NF-κB signaling: it generates the active subunit p50 and in its precursor form inhibits NF-κB activation. p105 processing occurs under basal conditions and increases following signaling. IκB kinase β (IKKβ) mediates phosphorylation at the C-terminal domain of p105, leads to accelerated processing and degradation of the precursor. Read More

    Improving glycemic control in model mice with type 2 diabetes by increasing superoxide dismutase (SOD) activity using silk fibroin hydrolysate (SFH).
    Biochem Biophys Res Commun 2017 Sep 15. Epub 2017 Sep 15.
    Department of Medical Genetics, College of Medicine, Hallym University, Chuncheon, Gangwon-do, 24252, Republic of Korea. Electronic address:
    Islet cell dysfunction in type 2 diabetes is primarily attributed to the increased apoptosis of pancreatic beta cells. Silk fibroin hydrolysate (SFH) has an effect on blood in type 2 diabetes model mice (C57BL/KsJ-db/db). However, its exact mechanism is unknown. Read More

    Detection of oligonucleotides by PNA-peptide conjugates recognizing the biarsenical fluorescein complex FlAsH-EDT2.
    Biochem Biophys Res Commun 2017 Sep 15. Epub 2017 Sep 15.
    Department of Pharmacy, University of Naples "Federico II", via Mezzocannone 16, 80134, Napoli, Italy. Electronic address:
    We report the application of the arsenical complex FlAsH-EDT2 for the identification of oligonucleotide sequences. We designed PNA sequences conjugated to either a tetracysteine motif and to split tetracysteine sequences, that are recognized by FlAsH. The effect of conjugation of the PNA to the tetracysteine peptide and RNA hybridization on the fluorescence of the arsenical complex has been investigated. Read More

    Adiponectin modulates the function of endothelial progenitor cells via AMPK/eNOS signaling pathway.
    Biochem Biophys Res Commun 2017 Sep 14. Epub 2017 Sep 14.
    Department of Geriatrics, Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200025, China. Electronic address:
    Endothelial progenitor cells have been shown to differentiate into endothelial cells and to play a pivotal role in vascular homeostasis. Adiponectin has anti-atherogenic and anti-inflammatory properties via directly acting on vascular cells. The aim of the present study is to explore the effect of adiponectin on major functions involved in survival, migration, and tube formation of endothelial progenitor cells and to explore the underlying mechanism. Read More

    COPS5 inhibition arrests the proliferation and growth of serous ovarian cancer cells via the elevation of p27 level.
    Biochem Biophys Res Commun 2017 Sep 15. Epub 2017 Sep 15.
    Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China; Department of Clinical Laboratory, Fudan University Shanghai Cancer Center, Shanghai, China. Electronic address:
    The fifth component of the COP9 signalosome complex (COPS5), which plays an essential role in ubiquitin-mediated protein degradation, has been found as a prognostic biomarker for multiple cancers, however, the role of COPS5 in serous ovarian cancer (SOC) remain to be clarified. In this study, we found that COPS5 expression was significantly increased in SOC cells and tissues compared with those controls. Mechanistically, COPS5 and p27was proved to interact with each other, with COPS5 acts as a negative regulator of p27. Read More

    Over-expression of PPARα in obese mice adipose tissue improves insulin sensitivity.
    Biochem Biophys Res Commun 2017 Sep 15. Epub 2017 Sep 15.
    Laboratory of Molecular Function of Food, Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto, Japan; Research Unit for Physiological Chemistry, Kyoto University, Kyoto, Japan. Electronic address:
    Peroxisome proliferator-activated receptor α (PPARα) is important in the regulation of lipid metabolism and expressed at high levels in the liver. Although PPARα is also expressed in adipose tissue, little is known about the relationship between its activation and the regulation of glucose metabolism. In this study, we developed adipose tissue specific PPARα over-expression (OE) mice. Read More

    Exosomes derived from human umbilical cord blood mesenchymal stem cells stimulates rejuvenation of human skin.
    Biochem Biophys Res Commun 2017 Sep 14. Epub 2017 Sep 14.
    Stem Cells and Regenerative Bioengineering Institute, Kangstem Biotech CO., LTD., 2nd Floor, Biotechnology Center, #81 Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 08826, South Korea. Electronic address:
    Human umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) play an important role in cutaneous wound healing, and recent studies suggested that MSC-derived exosomes activate several signaling pathways, which are conducive in wound healing and cell growth. In this study, we investigated the roles of exosomes that are derived from USC-CM (USC-CM Exos) in cutaneous collagen synthesis and permeation. We found that USC-CM has various growth factors associated with skin rejuvenation. Read More

    Cationization increases brain distribution of an amyloid-beta protofibril selective F(ab')2 fragment.
    Biochem Biophys Res Commun 2017 Sep 14. Epub 2017 Sep 14.
    Department of Public Health and Caring Sciences/Geriatrics, Uppsala University, Rudbeck Laboratory, SE-751 85 Uppsala, Sweden. Electronic address:
    Antibodies and fragments thereof are, because of high selectivity for their targets, considered as potential therapeutics and biomarkers for several neurological disorders. However, due to their large molecular size, antibodies/fragments do not easily penetrate into the brain. The aim of the present study was to improve the brain distribution via adsorptive-mediated transcytosis of an amyloid-beta (Aβ) protofibril selective F(ab')2 fragment (F(ab')2-h158). Read More

    Binding mode of metal ions to the bacterial iron import protein EfeO.
    Biochem Biophys Res Commun 2017 Sep 14. Epub 2017 Sep 14.
    Laboratory of Basic and Applied Molecular Biotechnology, Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611-0011, Japan. Electronic address:
    The tripartite EfeUOB system functions as a low pH iron importer in Gram-negative bacteria. In the alginate-assimilating bacterium Sphingomonas sp. strain A1, an additional EfeO-like protein (Algp7) is encoded downstream of the efeUOB operon. Read More

    The NAMPT/E2F2/SIRT1 axis promotes proliferation and inhibits p53-dependent apoptosis in human melanoma cells.
    Biochem Biophys Res Commun 2017 Sep 15. Epub 2017 Sep 15.
    Department of Pathophysiology, Zunyi Medical University, Zunyi, 563000, China. Electronic address:
    Melanoma is the most common primary malignant neoplasm in adults, causing more deaths than any other skin cancer, necessitating the development of new target-based approaches. Current evidence suggests SIRT1, the mammalian nicotinamide adenine dinucleotide (NAD(+))-dependent protein deacetylase, and nicotinamide phosphoribosyltransferase (NAMPT), the rate-limiting NAD(+) biosynthetic enzyme, together comprise a novel systemic regulatory network to play a pivotal role in cell proliferation and apoptosis. Nevertheless, how the regulation of this cofactor interfaces with signal transduction network remains poorly understood in melanoma. Read More

    ACAP3, the GTPase-activating protein specific to the small GTPase Arf6, regulates neuronal migration in the developing cerebral cortex.
    Biochem Biophys Res Commun 2017 Sep 15. Epub 2017 Sep 15.
    Department of Physiological Chemistry, Faculty of Medicine and Graduate School of Comprehensive Human Sciences, 1-1-1 Tennodai, Tsukuba 305-8578, Japan. Electronic address:
    The GTPase-activating protein (GAP) specific to the small GTPase Arf6, ACAP3, is known to regulate morphogenesis of neurons in vitro. However, physiological significance of ACAP3 in the brain development in vivo remains unclear. Here, we show that ACAP3 is involved in neuronal migration in the developing cerebral cortex of mice. Read More

    Identification of eukaryotic UDP-galactopyranose mutase inhibitors using the ThermoFAD assay.
    Biochem Biophys Res Commun 2017 Sep 15. Epub 2017 Sep 15.
    Department of Biochemistry, Virginia Tech, Blacksburg, VA 24061, USA; Virginia Tech Center for Drug Discovery, Virginia Tech, Blacksburg, VA 24061, USA. Electronic address:
    Aspergillus fumigatus is a human pathogen responsible for deadly infections in immune-compromised patients. A potential strategy for treating A. fumigatus infections is by targeting the biosynthesis of cell wall components, such as galactofuranase, which is absent in humans. Read More

    Biophysical characterization of the interaction between heme and proteins responsible for heme transfer in Streptococcus pyogenes.
    Biochem Biophys Res Commun 2017 Sep 14. Epub 2017 Sep 14.
    Department of Bioengineering, School of Engineering, The University of Tokyo, Bunkyo-ku, Tokyo, 113-8656 Japan; Medical Proteomics Laboratory, The Institute of Medical Science, The University of Tokyo, Minato-ku, Tokyo, 108-8639 Japan.
    Streptococcus pyogenes, an important pathogen that causes a wide range of diseases, possesses the sia gene cluster, which encodes proteins involved in the heme acquisition system. Although this system was previously described, the molecular mechanism of effective heme transfer remains to be elucidated. Here, we have characterized the interactions between heme and each domain of Streptococcal hemoprotein receptor (Shr) and Streptococcal heme-binding protein (Shp). Read More

    Changes in related circular RNAs following ERβ knockdown and the relationship to rBMSC osteogenesis.
    Biochem Biophys Res Commun 2017 Sep 15. Epub 2017 Sep 15.
    College of Pharmacy Jinan University, China. Electronic address:
    Recently, several studies have indicated that circular RNAs (circRNAs) play significant roles in various disease; however, little is known about the chronology of estrogen receptor beta (ERβ) deficiency and altered circRNA expression, or their relationship with osteogenesis. Herein, we show through western-blot and quantitative real-time PCR assays, that when ERβ is silenced, the expression of osteogenesis-related proteins and mRNAs were down-regulated. We then performed RNA-Seq to analyze differential circRNA expression between the control and ERβ knockdown group. Read More

    Apolipoprotein A-I attenuates LL-37-induced endothelial cell cytotoxicity.
    Biochem Biophys Res Commun 2017 Sep 15. Epub 2017 Sep 15.
    Department of Experimental Medical Science, Lund University, SE-221 84, Lund, Sweden. Electronic address:
    The human cathelicidin peptide LL-37 has antimicrobial and anti-biofilm functions, but LL-37 may also damage the host by triggering inflammation and exerting a cytotoxic effect, thereby reducing host cell viability. Human plasma mitigates LL-37-induced host cell cytotoxicity but the underlying mechanisms are not completely understood. Apolipoprotein A-I (ApoA-I) is a plasma protein endowed with atheroprotective effects. Read More

    The conformation change and tumor suppressor role of Merlin are both independent of Serine 518 phosphorylation.
    Biochem Biophys Res Commun 2017 Sep 15. Epub 2017 Sep 15.
    State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Protein Science, College of Life Sciences, Nankai University, 94 Weijin Road, Tianjin 300071, China. Electronic address:
    Merlin functions as a tumor suppressor and suppresses malignant activity of cancer cells through multiple mechanisms. However, whether Serine 518 phosphorylation regulates the conformation of Merlin as well as the open-closed conformational changes affect Merlin's tumor inhibitory activity remain controversial. In this study, we used different mutants to mimic related conformational states of Merlin and investigated its physiological functions. Read More

    Homocysteine upregulates interleukin-17A expression via NSun2-mediated RNA methylation in T lymphocytes.
    Biochem Biophys Res Commun 2017 Sep 15. Epub 2017 Sep 15.
    Department of Physiology and Pathophysiology, School of Basic Medical Science, Peking University Health Science Center, Key Laboratory of Molecular Cardiovascular Science, Ministry of Education, 38 Xueyuan Road, Beijing, 100191, PR China. Electronic address:
    Interleukin-17A (IL-17A) has been proven to participate in the process of various autoimmune diseases. The elevation of plasma homocysteine (Hcy), known as hyperhomocysteinemia (HHcy), is related to various chronic inflammatory diseases. Though HHcy-induced upregulation of IL-17A expression in T lymphocytes has been examined, the way in which IL-17A is regulated remains unclear. Read More

    Rapgef2, a guanine nucleotide exchange factor for Rap1 small GTPases, plays a crucial role in adherence junction (AJ) formation in radial glial cells through ERK-mediated upregulation of the AJ-constituent protein expression.
    Biochem Biophys Res Commun 2017 Sep 14. Epub 2017 Sep 14.
    Division of Molecular Biology, Department of Biochemistry and Molecular Biology, Kobe University Graduate School of Medicine, Kobe 650-0017, Japan. Electronic address:
    Rapgef2 and Rapgef6 define a subfamily of guanine nucleotide exchange factors for Rap1, characterized by possession of the Ras/Rap-associating domains and implicated in the etiology of schizophrenia. We previously found that dorsal telencephalon-specific Rapgef2 conditional knockout mice exhibits severe defects in formation of apical surface adherence junctions (AJs) and localization of radial glial cells (RGCs). In this study, we analyze the underlying molecular mechanism by using primary cultures of RGCs established from the developing cerebral cortex. Read More

    Multi-scale simulations of biological systems using the OPEP coarse-grained model.
    Biochem Biophys Res Commun 2017 Sep 14. Epub 2017 Sep 14.
    Laboratoire de Biochimie Théorique, UPR 9080, CNRS, Université Denis Diderot, Sorbonne Paris Cité, PSL Research University, IBPC, 13 Rue Pierre et Marie Curie, 75005, Paris, France. Electronic address:
    Biomolecules are complex machines that are optimized by evolution to properly fulfill or contribute to a variety of biochemical tasks in the cellular environment. Computer simulations based on quantum mechanics and atomistic force fields have been proven to be a powerful microscope for obtaining valuable insights into many biological, physical, and chemical processes. Many interesting phenomena involve, however, a time scale and a number of degrees of freedom, notably if crowding is considered, that cannot be explored at an atomistic resolution. Read More

    1 OF 1651