86,122 results match your criteria Biochemical and biophysical research communications[Journal]


Conformational changes of antitoxin HigA from Escherichia coli str. K-12 upon binding of its cognate toxin HigB reveal a new regulation mechanism in toxin-antitoxin systems.

Biochem Biophys Res Commun 2019 Apr 20. Epub 2019 Apr 20.

Multidiscipline Research Center, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing, 100049, China. Electronic address:

HigA functions as the antitoxin in HigB-HigA toxin-antitoxin system. It neutralizes HigB-mediated toxicity by forming a stable toxin-antitoxin complex. Here the crystal structure of isolated HigA from Escherichia coli str. Read More

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http://dx.doi.org/10.1016/j.bbrc.2019.04.061DOI Listing

Vaspin prevents myocardial injury in rats model of diabetic cardiomyopathy by enhancing autophagy and inhibiting inflammation.

Biochem Biophys Res Commun 2019 Apr 20. Epub 2019 Apr 20.

Department of Cardiology, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, 330006, China; Department of Cardiology, Qingdao Municipal Hospital, Qingdao, Shandong, 266071, China. Electronic address:

NLRP3 inflammasome activation plays an important role in diabetic cardiomyopathy (DCM). It is known that autophagy is related to the activation of inflammasomes during oxidative stress. Visceral adipose tissue-derived serine protease inhibitor (Vaspin), is an adipocytokine that has been shown to exert a protective effect on autophagic activity, but whether and how Vaspin improves myocardial damage in DCM remain unclear. Read More

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http://dx.doi.org/10.1016/j.bbrc.2019.04.110DOI Listing

Protein encapsulation in the hollow space of hemocyanin crystals containing a covalently conjugated ligand.

Biochem Biophys Res Commun 2019 Apr 20. Epub 2019 Apr 20.

Graduate School of Life Sciences, Tohoku University, Sendai, 980-8577, Japan; Faculty of Advanced Life Science, Hokkaido University, Sapporo, 060-0810, Japan; JST, PRESTO, Sendai, 980-8577, Japan. Electronic address:

Encapsulation of guest molecules into the vacant space of biomacromolecular crystals has been utilized for various purposes including functioning as a protein container to protect against physical stress and structural determination of the guest. Todarodes pacificus hemocyanin (TpHc) is a hollow cylindrical decameric protein complex with an inner space 110 Å in diameter and 160 Å in height. In the crystal, TpHc forms a straw-like bundle and contains one reactive Cys (Cys3246) in the inner domain of each protomer. Read More

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http://dx.doi.org/10.1016/j.bbrc.2019.04.062DOI Listing
April 2019
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The histone demethylase Kdm4 suppresses activation of hepatic stellate cell by inducing MiR-29 transcription.

Biochem Biophys Res Commun 2019 Apr 20. Epub 2019 Apr 20.

Key Laboratory of Targeted Intervention of Cardiovascular Disease and Innovative Collaboration Center for Cardiovascular Translational Medicine, Department of Pathophysiology, Nanjing Medical University, Nanjing, China; Institute of Biomedical Research, Liaocheng University, Liaocheng, China. Electronic address:

One of the hallmark events during liver fibrosis is the transition of quiescent hepatic stellate cells (HSC) into activated myofibroblasts, which are responsible for the production and deposition of pro-fibrogenic proteins. The epigenetic mechanism underlying HSC trans-differentiation is not fully understood. In the present study we investigated the contribution of histone H3K9 demethylase KDM4 in this process. Read More

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http://dx.doi.org/10.1016/j.bbrc.2019.04.105DOI Listing

Long non-coding RNA00882 contributes to platelet-derived growth factor-induced proliferation of human fetal airway smooth muscle cells by enhancing Wnt/β-catenin signaling via sponging miR-3619-5p.

Biochem Biophys Res Commun 2019 Apr 20. Epub 2019 Apr 20.

Yulin No.2 Hospital, Yulin City, Shaanxi, 719000, China.

Long non-coding RNAs (lncRNAs) are emerging as novel and critical regulators in the pathogenesis of asthma. However, the precise role of lncRNAs in pediatric asthma remains largely unknown. In this study, we aimed to investigate the biological function of lncRNA00882 (LINC00882) in regulating the proliferation of fetal airway smooth muscle (ASM) cells, which play an important role in airway remodeling during asthma development. Read More

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http://dx.doi.org/10.1016/j.bbrc.2019.04.106DOI Listing
April 2019
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ALOX12 inhibition sensitizes breast cancer to chemotherapy via AMPK activation and inhibition of lipid synthesis.

Biochem Biophys Res Commun 2019 Apr 20. Epub 2019 Apr 20.

Department of Breast Surgery, the Affiliated Tumor Hospital of Guangxi Medical University, Nanning, Guangxi, China. Electronic address:

Arachidonate lipoxygenase12 (Alox12) and its metabolites 12S-hydroxyeicosatetraenoic acid (12S-HETE) have been implicated in influencing tumor transformation and progression. In this study, we have systematically evaluated the expression, function and the downstream effectors of Alox12 in breast cancer using loss- and gain-of-function approaches. We demonstrated that both mRNA and protein levels of Alox12 were significantly increased in multiple breast cancer cell lines compared to normal breast cells. Read More

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http://dx.doi.org/10.1016/j.bbrc.2019.04.101DOI Listing

Long noncoding RNA CDKN2B-AS1 interacts with miR-411-3p to regulate ovarian cancer in vitro and in vivo through HIF-1a/VEGF/P38 pathway.

Biochem Biophys Res Commun 2019 Apr 20. Epub 2019 Apr 20.

Department of Gynaecology, Sichuan Academy of Medical Science & Sichuan Provincial People's Hospital, Chengdu, 610000, Sichuan, PR China. Electronic address:

Ovarian cancer (OC) is one of the most prevalent cancers with high fatality rate. In the present study, RT-PCR showed that the mRNA level of CDKN2B-AS1 was significantly upregulated while the miR-411-3p was downregulated in OC cell lines. In addition, the Sh-CDKN2B-AS1 resulted in the suppression of cell growth, invasion, migration and promotion of apoptosis, and miR-411-3p showed reversed results. Read More

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http://dx.doi.org/10.1016/j.bbrc.2019.03.141DOI Listing

Crystal structure of phyllogen, a phyllody-inducing effector protein of phytoplasma.

Biochem Biophys Res Commun 2019 Apr 17. Epub 2019 Apr 17.

Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan.

Phytoplasmas are plant pathogenic bacteria that often induce unique phyllody symptoms in which the floral organs are transformed into leaf-like structures. Recently, a novel family of bacterial effector genes, called phyllody-inducing genes (phyllogens), was identified as being involved in the induction of phyllody by degrading floral MADS-domain transcription factors (MTFs). However, the structural characteristics of phyllogens are unknown. Read More

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http://dx.doi.org/10.1016/j.bbrc.2019.04.060DOI Listing

Oligonucleotide-directed STAT3 alternative splicing switch drives anti-tumorigenic outcomes in MCF10 human breast cancer cells.

Biochem Biophys Res Commun 2019 Apr 19. Epub 2019 Apr 19.

Department of Biochemistry and Molecular Biology, Medical Building, University of Melbourne, Parkville, VIC, 3010, Australia. Electronic address:

Signal transducer and activator of transcription 3 (STAT3), a transcription factor responsive to the activation of cytokine receptors, is known for its oncogenic actions. Whilst STAT3α is the predominant spliceform in most tissues, alternative splicing of the STAT3 gene can generate a shorter STAT3β spliceform. Redirecting splicing to enhance STAT3β levels can result in tumor suppression in vivo, and so we evaluated the cellular basis underlying the anti-tumorigenic properties of STAT3β. Read More

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http://dx.doi.org/10.1016/j.bbrc.2019.04.054DOI Listing
April 2019
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Protopanaxadiol alleviates obesity in high-fat diet-fed mice via activation of energy-sensing neuron in the paraventricular nucleus of hypothalamus.

Biochem Biophys Res Commun 2019 Apr 19. Epub 2019 Apr 19.

School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China. Electronic address:

Obesity is one of the most important health problems worldwide. Panax ginseng has been reported to exert anti-obesity effect. However, the active constituents and the underlying mechanism remained uncertain. Read More

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http://dx.doi.org/10.1016/j.bbrc.2019.04.031DOI Listing

A novel antibody-drug conjugate, HcHAb18-DM1, has potent anti-tumor activity against human non-small cell lung cancer.

Biochem Biophys Res Commun 2019 Apr 19. Epub 2019 Apr 19.

National Translational Science Center for Molecular Medicine, Air Force Medical University, Xi'an, 710032, China; Department of Cell Biology, School of Basic Medicine, Air Force Medical University, Xi'an, 710032, China. Electronic address:

Cluster of differentiation 147 (CD147), a transmembrane protein of the immunoglobulin superfamily, is a potential target of treatment against human non-small cell lung cancer (NSCLC). Although there have been exciting advances in epidermal growth factor receptor (EGFR)-targeted therapy for NSCLC in recent years, additional novel targeted agents are needed to improve the efficiency and to offer more options for patients. Antibody-drug conjugates (ADCs) utilize a chemical linker to conjugate cytotoxic drugs to a monoclonal antibody to maximize the delivery to target cells and minimize the delivery to other normal cells. Read More

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http://dx.doi.org/10.1016/j.bbrc.2019.04.046DOI Listing

High glucose/ox-LDL induced hepatic sinusoidal capillarization via αvβ5/FAK/ERK signaling pathway.

Biochem Biophys Res Commun 2019 Apr 19. Epub 2019 Apr 19.

Department of Endocrinology, Gansu Provincial People's Hospital, 204 Donggang West Road, Lanzhou City, 730000, Gansu Province, China; Clinical Research Center for Metabolic Disease, Gansu Province, 204 Donggang West Road, Lanzhou City, 730000, Gansu Province, China. Electronic address:

Objective: The main purpose of this study is to explore the role of integrin αvβ5 in hepatic sinusoidal capillarization under high glucose/ox-LDL conditions.

Methods: Establish rat model of diabetic fatty liver disease. LSECs were extracted from tissue obtained from rats of control group, cultured and treated with media containing glucose (25 mM, 24 h)/ox-LDL (100 μg/ml, 24 h) in different inhibitors. Read More

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http://dx.doi.org/10.1016/j.bbrc.2019.04.082DOI Listing

Positive effect of exogenous brain-derived neurotrophic factor on impaired neurite development and mitochondrial function in dopaminergic neurons derived from dental pulp stem cells from children with attention deficit hyperactivity disorder.

Biochem Biophys Res Commun 2019 Apr 19. Epub 2019 Apr 19.

Section of Oral Medicine for Children, Division of Oral Health, Growth & Development, Faculty of Dental Science, Kyushu University, Maidashi 3-1-1, Higashi-Ku, Fukuoka, 812-8582, Japan. Electronic address:

Attention deficit hyperactivity disorder (ADHD) is one of the most common neurodevelopmental disorders and is characterized by impaired attention, hyperactivity, and impulsivity. While multiple etiologies are implicated in ADHD, its underlying mechanism(s) remain unclear. Although previous studies have suggested dysregulation of dopaminergic signals, mitochondria, and brain-derived neurotrophic factor (BDNF) in ADHD, few studies have reported these associations directly. Read More

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http://dx.doi.org/10.1016/j.bbrc.2019.04.084DOI Listing

HERC4 exerts an anti-tumor role through destabilizing the oncoprotein Smo.

Biochem Biophys Res Commun 2019 Apr 19. Epub 2019 Apr 19.

College of Life Sciences, Shandong Agricultural University, Tai'an, Shandong, China. Electronic address:

The GPCR-like transmembrane protein Smoothened (Smo) is an indispensable transducer in Hedgehog (Hh) pathway, its hyperactivation leads to several human cancers, including non-small cell lung cancer (NSCLC). The mechanism governing Smo stability still remains elusive. Here, we perform a modifier screening in Drosophila, and find that the E3 ligase dHerc4 degrades dSmo. Read More

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http://dx.doi.org/10.1016/j.bbrc.2019.04.113DOI Listing
April 2019
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ZFR promotes cell proliferation and tumor development in colorectal and liver cancers.

Biochem Biophys Res Commun 2019 Apr 19. Epub 2019 Apr 19.

Houston Methodist Research Institute, Houston Methodist Hospital, Houston, TX, USA; Houston Methodist Cancer Center, Houston Methodist Hospital, Houston, TX, USA. Electronic address:

Colorectal cancer (CRC) and liver cancer are the second and fourth leading causes of cancer-related deaths in the whole world, respectively, and each year over 1.6 million people die from these diseases. To identify driver genes in CRC and liver cancer, we have performed Sleeping Beauty transposon mutagenesis screens in mouse models. Read More

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http://dx.doi.org/10.1016/j.bbrc.2019.04.103DOI Listing

Silencing FOXO1 attenuates dexamethasone-induced apoptosis in osteoblastic MC3T3-E1 cells.

Biochem Biophys Res Commun 2019 Apr 19. Epub 2019 Apr 19.

Shandong Provincial Key Laboratory of Oral Tissue Regeneration, Department of Bone Metabolism, School of Stomatology Shandong University, Jinan, 250012, China. Electronic address:

Dexamethasone (DEX), a widely used glucocorticoid with strong anti-inflammatory and immunosuppressive activities, has been reported to induce apoptosis in osteoblasts, but the underlying mechanisms are still not comprehensively investigated. FOXO1 plays an important role in the regulation of cell proliferation and apoptosis. Our study aims to explore the role of FOXO1 in DEX-induced apoptosis of osteoblastic MC3T3-E1 cells through bioinformatics and experiments. Read More

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http://dx.doi.org/10.1016/j.bbrc.2019.04.112DOI Listing

2-D08 as a SUMOylation inhibitor induced ROS accumulation mediates apoptosis of acute myeloid leukemia cells possibly through the deSUMOylation of NOX2.

Biochem Biophys Res Commun 2019 Apr 19. Epub 2019 Apr 19.

Department of Hematology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China. Electronic address:

Acute myeloid leukemia (AML) is a heterogeneous clonal hematopoietic malignancy with poor survival and frequent relapse. Recently, a posttranslational modification of proteins with small ubiquitin-like modifiers (SUMO) has been notably implicated in a wide spectrum of diseases, especially cancers. Ubc9, as the sole E2-conjugating enzyme in SUMOylation cascade, particularly has been associated with adverse clinical outcomes. Read More

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http://dx.doi.org/10.1016/j.bbrc.2019.04.079DOI Listing

Creatine kinase muscle type specifically interacts with saturated fatty acid- and/or monounsaturated fatty acid-containing phosphatidic acids.

Biochem Biophys Res Commun 2019 Apr 19. Epub 2019 Apr 19.

Department of Chemistry, Graduate School of Science, Chiba University, Japan. Electronic address:

Diacylglycerol kinase (DGK) δ, which is a key enzyme in the pathogenesis of type 2 diabetes (T2D), preferentially generates saturated fatty acid (SFA)- and/or monounsaturated fatty acid (MUFA)-containing phosphatidic acids (PAs) such as 16:0/16:0-PA and 16:0/18:1-PA, but not polyunsaturated fatty acid (PUFA)-containing PAs, in glucose-stimulated myoblast cells. Here, we searched for the target proteins of 16:0/16:0-PA in the mouse skeletal muscle and identified an energy metabolizing enzyme, creatine kinase muscle type (CKM), which is correlated with T2D. CKM bound to 16:0/16:0-PA with the highest affinity (dissociation constant: 2. Read More

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http://dx.doi.org/10.1016/j.bbrc.2019.04.097DOI Listing

Link between cardiac function and the antioxidative defense mechanism in aged rats.

Biochem Biophys Res Commun 2019 Apr 19. Epub 2019 Apr 19.

Department of Physiology, Harbin Medical University, Harbin, China. Electronic address:

Aging presents profound structural and physiological changes in the cardiovascular system. Oxidative stress, a major contributing factor during the aging process, has been involved in various age-related cardiovascular pathologies. Nevertheless, the underlying mechanism of oxidative stress in the aging heart is still unclear. Read More

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http://dx.doi.org/10.1016/j.bbrc.2019.03.182DOI Listing

Tbx6 induces cardiomyocyte proliferation in postnatal and adult mouse hearts.

Biochem Biophys Res Commun 2019 Apr 19. Epub 2019 Apr 19.

Department of Cardiology, Faculty of Medicine, University of Tsukuba, 1-1-1, Tennoudai, Tsukuba City, Ibaraki, 305-8575, Japan. Electronic address:

Cardiovascular disease is a leading cause of death worldwide. Mammalian cardiomyocytes (CMs) proliferate during embryonic development, whereas they largely lose their regenerative capacity after birth. Defined factors expressed in cardiac progenitors or embryonic CMs may activate the cell cycle and induce CM proliferation in postnatal and adult hearts. Read More

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http://dx.doi.org/10.1016/j.bbrc.2019.04.087DOI Listing

Glyphosate induces toxicity and modulates calcium and NO signaling in zebrafish embryos.

Biochem Biophys Res Commun 2019 Apr 19. Epub 2019 Apr 19.

Ion Channel Biology Lab, Department of Biotechnology, Indian Institute of Technology Hyderabad (IITH), Kandi, Telangana, 502285, India. Electronic address:

Glyphosate, an herbicide used worldwide, has emerged as a pollutant. However, its toxic effects are debated by regulatory authorities. Therefore, it is essential to keep the use of such chemicals under continuous observation, and their effects must be re-evaluated. Read More

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http://dx.doi.org/10.1016/j.bbrc.2019.04.074DOI Listing

Electrical stimulation induces direct reprogramming of human dermal fibroblasts into hyaline chondrogenic cells.

Biochem Biophys Res Commun 2019 Apr 17. Epub 2019 Apr 17.

Department of Physical Therapy and Rehabilitation, College of Health Science, Eulji University, Seongnam, Republic of Korea. Electronic address:

The repair of articular cartilage needs a sufficient number of chondrocytes to replace the defect tissue. Direct reprogramming of fibroblasts into chondrocytes can provide a sufficient number of chondrocytes because fibroblasts can be expanded efficiently. Herein, we demonstrate for the first time that electrical stimulation can drive direct reprogramming of human dermal fibroblasts (HDFs) into hyaline chondrogenic cells. Read More

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http://dx.doi.org/10.1016/j.bbrc.2019.04.027DOI Listing
April 2019
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Knockdown of slincRAD leads to defective adipose development in vivo.

Biochem Biophys Res Commun 2019 Apr 17. Epub 2019 Apr 17.

State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Beijing, 100191, China. Electronic address:

The development of adipose tissue is a precisely coordinated cellular process, in which both protein-coding and non-coding genes are involved. To characterize the in vivo function of a novel long non-coding RNA (lncRNAs), loss-of-function assays were performed with slincRAD knockdown mice. Down-regulation of slincRAD expression was found to impair the development of adipose tissue, leading to a slim phenotype for both of the male and female mice. Read More

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http://dx.doi.org/10.1016/j.bbrc.2019.04.035DOI Listing
April 2019
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Inhibition of MEK5 suppresses TDP-43 toxicity via the mTOR-independent activation of the autophagy-lysosome pathway.

Biochem Biophys Res Commun 2019 Apr 17. Epub 2019 Apr 17.

Korea Brain Research Institute, Department of Neural Development and Disease, 61, Cheomdan-ro, Dong-gu, Daegu, 41062, Republic of Korea. Electronic address:

The most prominent hallmarks of many neurodegenerative diseases are the accumulation of misfolded protein aggregates and the death of certain neuronal populations. Autophagy is the major intracellular mechanism that degrades protein aggregates and damaged cellular components. Many studies have reported that the dysfunction of autophagy is associated with several neurodegenerative diseases, such as Alzheimer's disease, amyotrophic lateral sclerosis (ALS), and Parkinson's disease. Read More

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http://dx.doi.org/10.1016/j.bbrc.2019.04.088DOI Listing

Intraviral interactome of Chikungunya virus reveals the homo-oligomerization and palmitoylation of structural protein TF.

Biochem Biophys Res Commun 2019 Apr 17. Epub 2019 Apr 17.

Institute of Basic Medicine, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan, Shandong, 250062, PR China. Electronic address:

Chikungunya virus (CHIKV) is a re-emerging mosquito-transmitted RNA virus causing joint and muscle pain. Although the protein-protein interactions (PPIs) between nonstructural proteins of CHIKV have been extensively established, the complete CHIKV intraviral interactome remains to be elucidated. In this study, we examined all possible CHIKV intraviral PPIs by immunoprecipitation and constructed the intraviral interactome of CHIKV. Read More

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http://dx.doi.org/10.1016/j.bbrc.2019.04.098DOI Listing
April 2019
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Absence of mitochondrial DNA methylation in mouse oocyte maturation, aging and early embryo development.

Biochem Biophys Res Commun 2019 Apr 17. Epub 2019 Apr 17.

State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, PR China; University of Chinese Academy of Sciences, Beijing, 100101, PR China. Electronic address:

Mitochondrial DNA (mtDNA) is important for oxidative phosphorylation; dysfunctions can play a role in many mitochondrial diseases and can also affect the aging of cells and individuals. DNA methylation is an important epigenetic modification that plays a critical role in regulating gene expression. While recent studies have revealed the existence of mtDNA methylation there are still controversies about mtDNA methylation due to the special structure of mtDNA. Read More

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http://dx.doi.org/10.1016/j.bbrc.2019.04.100DOI Listing
April 2019
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Transient receptor potential vanilloid 4 is a critical mediator in LPS mediated inflammation by mediating calcineurin/NFATc3 signaling.

Biochem Biophys Res Commun 2019 Apr 17. Epub 2019 Apr 17.

Department of Anesthesiology, Affiliated People's Hospital of Jiangsu University, ZhengJang, 212002, China. Electronic address:

Transient Receptor Potential Vanilloid 4 (TRPV4) ion channel is thought to be an essential component of inflammatory response. However, its role and mechanism in regulating acute lung injury (ALI) and macrophages activation are not well characterized. In our study, we observe that blockade of TRPV4 using GSK2193874 or HC-067047 greatly improve the pneumonedema, the lung pathologic changes, the up-regulation of proinflammatory cytokines and the neutrophil infiltration in LPS-induced lung injury. Read More

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http://dx.doi.org/10.1016/j.bbrc.2019.04.020DOI Listing
April 2019
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CDK9 modulates circadian clock by attenuating REV-ERBα activity.

Biochem Biophys Res Commun 2019 Apr 17. Epub 2019 Apr 17.

CAS Key Laboratory of Tissue Microenvironment and Tumor, Shanghai Jiao Tong University School of Medicine, Shanghai Institute of Nutrition and Health, Shanghai Institute for Biological Sciences, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai, 200031, China. Electronic address:

Circadian clock and cell cycle are vital cellular programs acting in a timely-regulated, cyclic manner. The two cellular oscillators are coupled in various ways to facilitate biological processes. Here we report CDK9, a kinase belongs to the CDK family in regulating cell cycle and RNA Pol II activity, can serve as a modulator for circadian clock. Read More

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http://dx.doi.org/10.1016/j.bbrc.2019.04.043DOI Listing
April 2019
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HSP70/DNAJA3 chaperone/cochaperone regulates NF-κB activity in immune responses.

Biochem Biophys Res Commun 2019 Apr 17. Epub 2019 Apr 17.

Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, 980-8578, Japan. Electronic address:

Nuclear factor kappa B (NF-κB) controls the transcription of various genes in response to immune stimuli. Our previous study revealed that the Droj2/DNAJA3 cochaperone contributes to the NF-κB pathway in Drosophila and humans. In general, the cochaperone is associated with the 70-kDa heat shock protein (HSP70) chaperone and the complex supports the folding of diverse target proteins. Read More

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http://dx.doi.org/10.1016/j.bbrc.2019.04.077DOI Listing

Platelet microvesicles promote the recovery of neurological function in mouse model of cerebral infarction by inducing angiogenesis.

Biochem Biophys Res Commun 2019 Apr 17. Epub 2019 Apr 17.

Department of Neurology, The First Hospital of Xi'an, Xi'an, China. Electronic address:

The aim of this study is to investigate the effect of PMVs on mice with ischemic cerebral infarction and its mechanism. Male C57BL/6 mice were selected, and the right focal cortical infarction model was established via cauterization under a microscope and randomly divided into sham operation (Sham) group, normal saline control (Saline) group and platelet microvesicles intervention (PMVs) group. At 1 h after modeling, 5 μL of PMVs (50 μg/mL) or normal saline was injected into the lateral ventricle. Read More

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http://dx.doi.org/10.1016/j.bbrc.2019.04.083DOI Listing
April 2019
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The circRNA circP4HB promotes NSCLC aggressiveness and metastasis by sponging miR-133a-5p.

Biochem Biophys Res Commun 2019 Apr 17. Epub 2019 Apr 17.

Anhui Clinical and Preclinical Key Laboratory of Respiratory Disease, Department of Respiration, First Affiliated Hospital, Bengbu Medical College, Bengbu, Anhui Province, China. Electronic address:

Background: Non-small cell lung carcinoma (NSCLC) continues to top the list of cancer mortalities worldwide. The role of circular RNAs (circRNAs) in tumorigenesis has been increasingly appreciated, although it is relatively unexplored in NSCLC. Herein, we report on the role of circP4HB in NSCLC. Read More

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http://dx.doi.org/10.1016/j.bbrc.2019.04.108DOI Listing
April 2019
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Inhibition of GIP signaling extends lifespan without caloric restriction.

Biochem Biophys Res Commun 2019 Apr 17. Epub 2019 Apr 17.

Department of Endocrinology, Diabetes and Geriatric Medicine, Akita University Graduate School of Medicine, Japan. Electronic address:

Aims/introduction: Caloric restriction (CR) promotes longevity and exerts anti-aging effects by increasing Sirtuin production and activation. Gastric inhibitory polypeptide (GIP), a gastrointestinal peptide hormone, exerts various effects on pancreatic β-cells and extra-pancreatic tissues. GIP promotes glucose-dependent augmentation of insulin secretion and uptake of nutrients into the adipose tissue. Read More

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http://dx.doi.org/10.1016/j.bbrc.2019.04.036DOI Listing
April 2019
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Corrigendum to 'Long non-coding RNA Linc00320 inhibits glioma cell proliferation through restraining Wnt/β-catenin signaling', Biochem Biophys Res Commun. 2019 Jan 8; 508(2):458-464.

Biochem Biophys Res Commun 2019 Apr 17. Epub 2019 Apr 17.

Department of Functional Neurosurgery, Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, No. 197 Ruijin Second Road, Shanghai, 200240, China. Electronic address:

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http://dx.doi.org/10.1016/j.bbrc.2019.04.071DOI Listing
April 2019
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Determinants of lentiviral Vpx-CRL4 E3 ligase-mediated SAMHD1 degradation in the substrate adaptor protein DCAF1.

Biochem Biophys Res Commun 2019 Apr 17. Epub 2019 Apr 17.

Institute of Virology and AIDS Research, The First Hospital of Jilin University, Changchun, 130021, China; Institute of Translational Medicine, The First Hospital of Jilin University, Changchun, 130021, China. Electronic address:

The lentiviral accessory protein Vpx enhances viral replication in macrophages, dendritic cells and resting CD4 T cells by utilizing the host CRL4-DCAF1 E3 ligase to trigger the degradation of the intrinsic antiviral factor SAMHD1. Distinct from the species-specific recognition of either the N or C-terminus of SAMHD1 by Vpx proteins of different HIV-2 and SIV lineages, Vpx recruits SAMHD1 onto the same CRL4-DCAF1 complex. However, the determinants in DCAF1 that are required for Vpx-mediated SAMHD1 degradation have not been well characterized. Read More

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http://dx.doi.org/10.1016/j.bbrc.2019.04.085DOI Listing
April 2019
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Long non-coding RNA LOXL1-AS1 acts as a ceRNA for miR-324-3p to contribute to cholangiocarcinoma progression via modulation of ATP-binding cassette transporter A1.

Biochem Biophys Res Commun 2019 Apr 16. Epub 2019 Apr 16.

Department of Gastroenterology, The Second Affiliated Hospital of Qiqihar Medical University, Qiqihar, 161000, China. Electronic address:

Accumulating evidence has appreciated long non-coding RNAs (lncRNAs) as novel prognostic markers and therapeutic targets in malignant carcinomas. Here, we aim to investigate the value of a novel cancer-related lncRNA, LOXL1-AS1, in cholangiocarcinoma (CCA). LOXL1-AS1 was found overexpressed in CCA tissues screened by high-throughput sequencing technology. Read More

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http://dx.doi.org/10.1016/j.bbrc.2019.04.089DOI Listing
April 2019
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Prenylated Rab acceptor RABAC1 inhibits anti-apoptotic protein BCL2A1 and induces apoptosis.

Biochem Biophys Res Commun 2019 Apr 16. Epub 2019 Apr 16.

Immunotherapy Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea; Department of Biomolecular Science, University of Science and Technology, Daejeon, Republic of Korea. Electronic address:

The B cell lymphoma 2 (BCL2) family of proteins constitutes a critical intracellular checkpoint in the intrinsic apoptosis pathway. Among BCL2 members, the anti-apoptotic protein BCL2A1 mediates the resistance to BCL2 inhibitors and may be considered as a target for anti-cancer therapy. Here, we report that prenylated Rab acceptor 1 (RABAC1 or PRA1) inhibits the anti-apoptotic activity of BCL2A1 and induces apoptosis in AGS gastric cancer cells. Read More

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http://dx.doi.org/10.1016/j.bbrc.2019.04.080DOI Listing
April 2019
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NIPA2 regulates osteoblast function via its effect on apoptosis pathways in type 2 diabetes osteoporosis.

Biochem Biophys Res Commun 2019 Apr 16. Epub 2019 Apr 16.

Department of Orthopedics, The First Hospital of China Medical University, Shenyang, Liaoning, China. Electronic address:

Type 2 diabetes osteoporosis has recently become a hot topic in the study of diabetic complications, but the specific mechanism of its development remains unclear. Non-imprinted in Prader-Willi/Angelman syndrome region protein 2 (NIPA2), a highly-selective magnesium ion transporter, has been found to be associated with type 2 diabetes. In this study we aimed to investigate the specific role and mechanism of NIPA2 in the pathogenesis of type 2 diabetes osteoporosis. Read More

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http://dx.doi.org/10.1016/j.bbrc.2019.04.030DOI Listing
April 2019
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Tetrandrine induces apoptosis in human neuroblastoma through regulating the Hippo/YAP signaling pathway.

Biochem Biophys Res Commun 2019 Apr 16. Epub 2019 Apr 16.

Department of Oncology, The First Affiliated Hospital, Xi'an Jiaotong University, 277 Yanta West Road, Xi'an, 710061, Shaanxi, China. Electronic address:

Tetrandrine (TET), a bis-benzylisoquinoline alkaloid, shows cytotoxicity against several different types of tumors. However, the mechanism by which TET exerts its anti-cancer capabilities remains unclear. In this study, we confirmed that TET inhibits proliferation and induces apoptosis in neuroblastoma (NB) in vitro and in vivo. Read More

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http://dx.doi.org/10.1016/j.bbrc.2019.04.075DOI Listing

Construction of the common cortical space by spontaneous activity and its application in the mouse cortex.

Biochem Biophys Res Commun 2019 Apr 16. Epub 2019 Apr 16.

Department of Mental Disorder Research, National Institute of Neuroscience, National Center of Neurology and Psychiatry (NCNP), 4-1-1 Ogawa-Higashi, Kodaira, Tokyo, 187-8502, Japan.

Wide-field optical imaging of the animal brain is a useful technique for measuring brain dynamics, including spatial structure. However, quantitative inter-animal comparison is difficult due to lack of the common cortical space that can normalize individually imaged brains as done in human functional MRI studies. Here, by using wide-field functional Ca imaging on anesthetized transgenic mice expressing G-CaMP7 in astrocytes and excitatory neutrons, we attempted to establish the common cortical space in mice, which can be useful as a standard of functional brain map. Read More

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http://dx.doi.org/10.1016/j.bbrc.2019.04.048DOI Listing
April 2019
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Single-molecule nanomechanical spectroscopy shows calcium ions contribute to chain association and structural flexibility of blood clotting factor VIII.

Biochem Biophys Res Commun 2019 Apr 16. Epub 2019 Apr 16.

CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, 201800, China; ZhangJiang Lab, Shanghai Synchrotron Radiation Facility, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai, 201210, China. Electronic address:

Blood coagulation factor VIII (FVIII) can bind calcium ions and ion-protein interactions appear central importance for both their structure and function in coagulation cascade. However, the mechanism and details of how calcium dependent structure change of proteins to fulfill their function remain to be fully defined. In this work, PeakForce Quantitative Nanomechanics (PF-QNM) mode atomic force microscopy (AFM) was used to map the topography and mechanical properties of FVIII with single protein resolution under different calcium concentrations. Read More

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http://dx.doi.org/10.1016/j.bbrc.2019.04.068DOI Listing
April 2019
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Microglia promote the proliferation of neural precursor cells by secreting osteopontin.

Biochem Biophys Res Commun 2019 Apr 16. Epub 2019 Apr 16.

Department of Molecular Pharmacology, National Institute of Neuroscience, National Center of Neurology and Psychiatry, 4-1-1 Ogawa-higashi, Kodaira, Tokyo, 187-8502, Japan. Electronic address:

Microglia are central nervous system-resident immune cells that play a crucial role in brain development by interacting with neural precursor cells (NPCs). It has been reported that microglia regulate the number of NPC by phagocytosis, inducing apoptosis, and promoting proliferation. Microglia surrounding the subventricular zone express osteopontin (OPN) during brain development. Read More

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http://dx.doi.org/10.1016/j.bbrc.2019.04.076DOI Listing
April 2019
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MicroRNA-146b-5p protects oligodendrocyte precursor cells from oxygen/glucose deprivation-induced injury through regulating Keap1/Nrf2 signaling via targeting bromodomain-containing protein 4.

Biochem Biophys Res Commun 2019 Apr 16. Epub 2019 Apr 16.

Institute of Neurobiology, The Medical College of Xi'an Jiaotong University, Xi'an, Shaanxi, 710061, PR China.

The injury of oligodendrocyte precursor cells (OPCs) contributes to the pathology of hypoxic-ischemic encephalopathy in newborns. MicroRNAs (miRNAs) have emerge as critical regulators of hypoxic-ischemic encephalopathy; however, the role of miRNAs in regulating OPC injury remains largely unknown. MiRNA-146b-5p (miR-146b-5p) has been reported to exert a cytoprotective function under various pathological conditions. Read More

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http://dx.doi.org/10.1016/j.bbrc.2019.04.045DOI Listing
April 2019
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Process of immunogenic cell death caused by disulfiram as the anti-colorectal cancer candidate.

Biochem Biophys Res Commun 2019 Apr 16. Epub 2019 Apr 16.

Department of Immunology, School of Life Sciences and Biopharmaceuticals Engineering, Guangdong Pharmaceutical University, Guangzhou, 510006, PR China; Guangdong Engineering & Technology Research Center of Topical Precise Drug Delivery System, Guangdong Pharmaceutical University, Guangzhou, 510006, PR China; Key Laboratory of Digital Quality Evaluation of Chinese Medical of State Administration of TCM, Guangzhou, 510006, PR China. Electronic address:

Background: Disulfiram (DSF), a drug widely used to control alcoholism, which has anticancer activity by inducing apoptosis in a copper (Cu)-dependent manner. Numerous evidences from mouse experiments indicated that some anti-cancer agents of chemotherapeutic drugs favor the induction of immunogenic cancer cell death (ICD) leading to tumor-specific immune responses. However, whether DSF could induce the colorectal tumor cells death and the mechanism involved in ICD regulatory remains elusive. Read More

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http://dx.doi.org/10.1016/j.bbrc.2019.03.192DOI Listing
April 2019
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Effects of epigallocatechin-3-gallate on the HMGB1/RAGE pathway in PM-exposed asthmatic rats.

Biochem Biophys Res Commun 2019 Apr 16. Epub 2019 Apr 16.

Department of Pediatrics, Children's Hospital Affiliated of Zhengzhou University, No.33 Long hu Wai huan East Road, Zhengzhou, 450018, Henan, China. Electronic address:

Current studies have shown that long-term exposure to fine particulate matter (PM) can aggravate lung injury in asthmatic children. The HMGB1/RAGE pathway may play an important role, but few studies on the HMGB1/RAGE signaling pathway in PM-induced asthma have been performed. Epigallocatechin-3-gallate (EGCG), which has antioxidant, anti-inflammatory and immunomodulatory effects, has not been examined in studies at home and abroad. Read More

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http://dx.doi.org/10.1016/j.bbrc.2019.03.165DOI Listing
April 2019
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Exosomes derived from plasma of septic patients inhibit apoptosis of T lymphocytes by down-regulating bad via hsa-miR-7-5p.

Biochem Biophys Res Commun 2019 Apr 16. Epub 2019 Apr 16.

Department of Respiratory Medicine, Chinese PLA General Hospital, 28th Fuxing Road, Beijing, 100853, PR China. Electronic address:

Immunosuppression is currently a vital pathophysiological characteristic and core problem of sepsis. Apoptosis of T lymphocyte contribute to immunosuppression by decreasing immune effector cells. A report has recently revealed the potential regulatory role of exosomal miRNAs derived from plasma of septic patients on immune system, but the underlying mechanism is unclear. Read More

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http://dx.doi.org/10.1016/j.bbrc.2019.04.051DOI Listing

Arsenic trioxide inhibits EMT in hepatocellular carcinoma by promoting lncRNA MEG3 via PKM2.

Biochem Biophys Res Commun 2019 Apr 16. Epub 2019 Apr 16.

Department of Interventional Radiology, Zhongshan Hospital, Fudan University, China. Electronic address:

Hepatocellular carcinoma (HCC) presents a great burden for patients worldwide, and metastasis of HCC remains problematic. Arsenic trioxide is a traditional drug that has shown excellent efficacy when applied as cancer therapy. Our study explored the antimetastatic mechanism of arsenic trioxide in HCC. Read More

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http://dx.doi.org/10.1016/j.bbrc.2019.04.081DOI Listing
April 2019
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Dysregulation of humoral immunity in Foxp3 conditional-knockout mice.

Biochem Biophys Res Commun 2019 Apr 15. Epub 2019 Apr 15.

Laboratory of Pharmaceutical Immunology, Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba, 278-8510, Japan. Electronic address:

Foxp3 regulatory T cells (Tregs) are crucial for maintaining tolerance to self-antigens and preventing autoimmune diseases. Loss of Foxp3 expression leads to autoimmunity and disrupts humoral immune responses, including hyperproduction of immunoglobulin E (IgE). Elucidation of how Tregs control antibody production can lead to the development of new therapies for autoimmune and allergic diseases. Read More

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http://dx.doi.org/10.1016/j.bbrc.2019.04.090DOI Listing
April 2019
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Diastereomers of a mono-substituted phosphoryl guanidine trideoxyribonucleotide: Isolation and properties.

Biochem Biophys Res Commun 2019 Apr 15. Epub 2019 Apr 15.

Institute of Chemical Biology and Fundamental Medicine, SB RAS, 8 Lavrentiev Avenue, Novosibirsk, 630090, Russia; Novosibirsk State University, 2 Pirogova St., Novosibirsk, 630090, Russia.

Recently, a new type of nucleic acid analogues with modified phosphate group, namely, phosphoryl guanidine oligonucleotides, has been described. In the present work, we assess the difference between diastereomers of a mono-substituted phosphoryl guanidine oligonucleotide and analyze their resistance to nuclease digestion. Individual diastereomers ('fast' and 'slow') of a trideoxynucleotide d (TpCp*A) were isolated by reverse-phase HPLC. Read More

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http://dx.doi.org/10.1016/j.bbrc.2019.04.024DOI Listing
April 2019
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IGF2BP1 promotes LPS-induced NFκB activation and pro-inflammatory cytokines production in human macrophages and monocytes.

Biochem Biophys Res Commun 2019 Apr 15. Epub 2019 Apr 15.

Department of Stomatology, Nanfang Hospital and College of Stomatology, Southern Medical University, Guangzhou, China. Electronic address:

Background: Lipopolysaccharide (LPS)-induced macrophage/monocyte activation and pro-inflammatory cytokines production are important mediators for periodontitis progression. The current study tested the potential role of insulin-like growth factor 2 mRNA-binding protein 1 (IGF2BP1) in the process.

Methods: THP-1 human macrophages and primary human peripheral blood mononuclear cells (PBMCs) were treated with LPS. Read More

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http://dx.doi.org/10.1016/j.bbrc.2019.03.206DOI Listing
April 2019
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Sestrin 2 protects against metabolic stress in a p53-independent manner.

Biochem Biophys Res Commun 2019 Apr 16. Epub 2019 Apr 16.

Department of Biochemistry & Molecular Biology, China Medical University, Shenyang, 110026, China; Key Laboratory of Cell Biology, Ministry of Public Health, And Key Laboratory of Medical Cell Biology, Ministry of Education, China Medical University, Shenyang, 110026, China. Electronic address:

Glucose limitation activates p53, which functions as an adaptive response to maintain cell survival. However, p53 is frequently deleted or mutated in a variety of tumors, while most cancer cells can acclimatize themselves to metabolically unfavorable surrounding, indicating that alternative mechanisms other than p53 transactivation underly adaptive response of cancer cells with p53 deletion or mutation to metabolically hostile environment. Sestrin 2 (SESN2) is a p53 downstream target, which plays a protective role against various stressful stimuli, such as genotoxic, energetic, and oxidative stress. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S0006291X193070
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http://dx.doi.org/10.1016/j.bbrc.2019.04.072DOI Listing
April 2019
2 Reads