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    83477 results match your criteria Biochemical and biophysical research communications[Journal]

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    Lis1 dysfunction leads to traction force reduction and cytoskeletal disorganization during cell migration.
    Biochem Biophys Res Commun 2018 Feb 19. Epub 2018 Feb 19.
    Institute of Brain Science, School of Medicine, National Yang-Ming University, Taipei 112, Taiwan, ROC; Brain Research Center (BRC) and Biophotonics and Molecular Imaging Research Center (BMIRC), National Yang-Ming University, Taipei 112, Taiwan, ROC. Electronic address:
    Cell migration is a critical process during development, tissue repair, and cancer metastasis. It requires complex processes of cell adhesion, cytoskeletal dynamics, and force generation. Lis1 plays an important role in the migration of neurons, fibroblasts and other cell types, and is essential for normal development of the cerebral cortex. Read More

    Redox exchange of the disulfides of human two-domain CD4 regulates the conformational dynamics of each domain, providing insight into its mechanisms of control.
    Biochem Biophys Res Commun 2018 Feb 19. Epub 2018 Feb 19.
    HIV Pathogenesis Research Unit, Department of Molecular Medicine and Haematology, Faculty of Health Sciences, University of the Witwatersrand, 7 York Road, Parktown, 2193, Johannesburg, South Africa.
    CD4, a membrane glycoprotein expressed by specific leukocytes, plays a vital role in the human immune response and acts as a primary receptor for HIV entry. Of its four ecto-domains (D1-D4), D1, D2, and D4 each contain a distinctive disulfide bond. Whereas the disulfides of D1 and D4 are more traditional in nature, providing structural functions, that of D2 is referred to as an "allosteric" disulfide due to its high dihedral strain energy and relative ease of reduction that is thought to regulate CD4 structure and function by shuffling its redox state. Read More

    Shear-dependent fibrillogenesis of fibronectin: Impact of platelet integrins and actin cytoskeleton.
    Biochem Biophys Res Commun 2018 Feb 19. Epub 2018 Feb 19.
    Department of General, Visceral and Pediatric Surgery, University Hospital and Medical Faculty of the Heinrich-Heine University Düsseldorf, Düsseldorf, Germany.
    Soluble plasma fibronectin (Fn) with its inactive compact structure requires unfolding to assemble into active fibrils, which play a role in hemostasis and thrombosis. Fn fibril assembly involves Fn binding to cell receptors, biomechanical coupling of Fn to the cytoskeleton by integrins, exposure of self-assembly sites via contractile cell forces, and elongation of fibrils by Fn polymerization. In this report, we investigated the effect of platelet integrins and actin cytoskeleton on conformational changes of Fn induced by shear. Read More

    Angiotensin-II regulates dosing time-dependent intratumoral accumulation of macromolecular drug formulations via 24-h blood pressure rhythm in tumor-bearing mice.
    Biochem Biophys Res Commun 2018 Feb 19. Epub 2018 Feb 19.
    Department of Pharmaceutics, Graduate School of Pharmaceutical Sciences, Kyushu University, Higashi-ku, Fukuoka, 812-8512, Japan.
    One approach to increasing pharmacotherapy effects is administering drugs at times of day when they are most effective and/or best tolerated. Circadian variation in expression of pharmacokinetics- and pharmacodynamics-related genes was shown to contribute to dosing time-dependent differences in therapeutic effects of small molecule drugs. However, influence of dosing time of day on effects of high molecular weight formulations, such as drugs encapsulated in liposomes, has not been studied in detail. Read More

    Silencing of LncRNA-HOTAIR decreases drug resistance of Non-Small Cell Lung Cancer cells by inactivating autophagy via suppressing the phosphorylation of ULK1.
    Biochem Biophys Res Commun 2018 Feb 19. Epub 2018 Feb 19.
    Department of Respiratory and Critical Care Medicine, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, Sichuan 610072, China.
    Drug resistance is an important factor leading to the recurrence and metastasis of Non-Small Cell Lung Cancer (NSCLC). Long non-coding RNAs (LncRNAs) play important roles in drug resistance of tumor cells. The aim of our study was to investigate the mechanism of LncRNA-HOTAIR in regulating drug resistance of NSCLC cells. Read More

    Cytoplasmic p53 contributes to the removal of uracils misincorporated by HIV-1 reverse transcriptase.
    Biochem Biophys Res Commun 2018 Feb 19. Epub 2018 Feb 19.
    Infectious Diseases Unit, Sheba Medical Center, Tel-Hashomer 5265601, Israel. Electronic address:
    HIV-1 reverse transcriptase (RT) in the cytoplasm of HIV-infected cells efficiently inserts the non-canonical dUTP into the proviral DNA, and extends the dU-terminated DNA. The misincorporation of dUTP leads to mutagenesis, and uracils can down-regulate viral gene expression. However, uracilation might also protect HIV DNA from auto-integration in the cytoplasm. Read More

    Ribosome biogenesis protein Urb2 regulates hematopoietic stem cells development via P53 pathway in zebrafish.
    Biochem Biophys Res Commun 2018 Feb 19. Epub 2018 Feb 19.
    Key Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education, Laboratory of Molecular Developmental Biology, School of Life Sciences, Southwest University, Beibei, 400715 Chongqing, China. Electronic address:
    Ribosome biogenesis is a significant process in cells. Dysfunction in this process will result in the defects of protein synthesis and consequently cause the development of specific diseases called ribosomopathies. Mutations in ribosome biogenesis protein Rps19, Rpl5, or Rpl11 can lead to hematopoietic defects in human, thus triggering the disease Diamond Blackfan anemia. Read More

    Development of a functional thyroid model based on an organoid culture system.
    Biochem Biophys Res Commun 2018 Feb 19. Epub 2018 Feb 19.
    Division of Gene Regulation, Institute for Advanced Medical Research, School of Medicine, Keio University, 35 Shinano-machi, Shinjuku-ku, Tokyo 160-8582, Japan. Electronic address:
    The low turnover rate of thyroid follicular cells and the lack of a long-term thyroid cell culture system have hampered studies of thyroid carcinogenesis. We have now established a thyroid organoid culture system that supports thyroid cell proliferation in vitro. The established mouse thyroid organoids performed thyroid functions including thyroglobulin synthesis, iodide uptake, and the production and release of thyroid hormone. Read More

    RIP3 deficiency protects against traumatic brain injury (TBI) through suppressing oxidative stress, inflammation and apoptosis: Dependent on AMPK pathway.
    Biochem Biophys Res Commun 2018 Feb 19. Epub 2018 Feb 19.
    Department of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan, 430060, Hubei, China.
    Traumatic brain injury (TBI) is a leading cause of disability and mortality in young adults worldwide. The pathophysiology is not fully understood. Programmed necrosis (necroptosis) is a newly identified mechanism of cell death combining features of both apoptosis and necrosis. Read More

    GhoT of the GhoT/GhoS toxin/antitoxin system damages lipid membranes by forming transient pores.
    Biochem Biophys Res Commun 2018 Feb 19. Epub 2018 Feb 19.
    Department of Chemical Engineering, Pennsylvania State University, University Park, PA, 16802-4400, USA. Electronic address:
    GhoT is a bacterial toxin of the type V toxin/antitoxin system that allows Escherichia coli to reduce its metabolism in response to oxidative and bile stress. GhoT functions by increasing membrane permeability and reducing both ATP levels and the proton motive force. However, how GhoT damages the inner membrane has not been elucidated. Read More

    Graphene coating on the surface of CoCrMo alloy enhances the adhesion and proliferation of bone marrow mesenchymal stem cells.
    Biochem Biophys Res Commun 2018 Feb 19. Epub 2018 Feb 19.
    Department of Orthopedics, Xijing Hospital, The Fourth Military Medical University, Xi'an, PR China. Electronic address:
    The objective was to investigate whether a graphene coating could improve the surface bioactivity of a cobalt-chromium-molybdenum-based alloy (CoCrMo). Graphene was produced by chemical vapor deposition and transferred to the surface of the CoCrMo alloy using an improved wet transfer approach. The morphology of the samples was observed, and the adhesion force and stabilization of graphene coating were analyzed by a nanoscratch test and ultrasonication test. Read More

    Transcriptional information revealed differentially expressed circular RNAs in facet joint osteoarthritis.
    Biochem Biophys Res Commun 2018 Feb 19. Epub 2018 Feb 19.
    Department of Spine Surgery, The Second Affiliated Hospital of Nantong University, Nantong, Jiangsu, China. Electronic address:
    Circular RNAs are newly identified RNAs that regulate gene expression and execute various biological activities. Facet joint osteoarthritis (FJOA) is a severe facet joint cartilage degeneration disease that induces chronic low back pain to a larger population. The molecular mechanisms of FJOA, especially the presence and changes of circular RNAs in FJOA have not been investigated. Read More

    Identification of matrix metalloproteinase 9-interacting sequences in staphylococcal superantigen-like protein 5.
    Biochem Biophys Res Commun 2018 Feb 17. Epub 2018 Feb 17.
    Department of Molecular and Cellular Health Sciences, Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe-Dori, Mizuho-ku, Nagoya, 467-8603, Japan.
    Staphylococcal superantigen like 5 (SSL5) is an exotoxin produced by S. aureus and has a strong inhibitory effect on MMP-9 enzymatic activity. However, the mechanism of inhibition remains unclear. Read More

    Additional increased effects of mannitol-temozolomide combined treatment on blood-brain barrier permeability.
    Biochem Biophys Res Commun 2018 Feb 17. Epub 2018 Feb 17.
    Department of Neurology, CHA Bundang Medical Center, CHA University, Seongnam, Republic of Korea; Institute for Clinical Research, CHA Bundang Medical Center, CHA University, Seongnam, Republic of Korea. Electronic address:
    The blood-brain barrier (BBB) is major obstacle in drug or stem cell treatment in chronic stroke. We hypothesized that adding mannitol to temozolomide (TMZ) is a practically applicable method for resolving the low efficacy of intravenous mannitol therapy. In this study, we investigated whether BBB permeability could be increased by this combined treatment. Read More

    Direct posttranslational modification of astrocytic connexin 43 proteins by the general anesthetic propofol in the cerebral cortex.
    Biochem Biophys Res Commun 2018 Feb 17. Epub 2018 Feb 17.
    Department of Pharmacology, School of Medicine, Keio University, Japan.
    Propofol is widely used as a general anesthetic and is generally considered to exert its action by regulating neuronal firing via facilitation of GABAreceptors. However, accumulating evidence suggests that propofol also acts on astrocytes, including inhibitory effects on gap junctional coupling, but the underlying molecular mechanisms remain largely unknown. Here, using acute cortical brain slices prepared from mice of either sex, we characterize propofol-induced molecular changes in astrocytic gap junction protein connexin 43 (Cx43). Read More

    Human AK2 links intracellular bioenergetic redistribution to the fate of hematopoietic progenitors.
    Biochem Biophys Res Commun 2018 Feb 17. Epub 2018 Feb 17.
    Department of Clinical Application, Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto, Kyoto, 6068507, Japan. Electronic address:
    AK2 is an adenylate phosphotransferase that localizes at the intermembrane spaces of the mitochondria, and its mutations cause a severe combined immunodeficiency with neutrophil maturation arrest named reticular dysgenesis (RD). Although the dysfunction of hematopoietic stem cells (HSCs) has been implicated, earlier developmental events that affect the fate of HSCs and/or hematopoietic progenitors have not been reported. Here, we used RD-patient-derived induced pluripotent stem cells (iPSCs) as a model of AK2-deficient human cells. Read More

    Soluble dietary fiber improves energy homeostasis in obese mice by remodeling the gut microbiota.
    Biochem Biophys Res Commun 2018 Feb 17. Epub 2018 Feb 17.
    Intensive Care Unit, Shengjing Hospital of China Medical University, Shenyang, Liaoning, China. Electronic address:
    Intervention with dietary fibers is an important strategy to combat the global epidemic of obesity which is a consequence of energy imbalance. However, a possible role of the gut microbiota in effects of dietary fibers on energy homeostasis remains unclear. Here, we treated a high fat diet-induced obese (DIO) mouse model with soluble dietary fiber. Read More

    Overexpression of Larp4B downregulates dMyc and reduces cell and organ sizes in Drosophila.
    Biochem Biophys Res Commun 2018 Feb 17. Epub 2018 Feb 17.
    Department of Biological Sciences, Tokyo Metropolitan University, 1-1 Minami-osawa, Hachioji-shi, Tokyo 192-0397, Japan. Electronic address:
    Regulation of cell and organ sizes is fundamental for all organisms, but its molecular basis is not fully understood. Here we performed a gain-of-function screen and identified larp4B whose overexpression reduces cell and organ sizes in Drosophila melanogaster. Larp4B is a member of La-related proteins (LARPs) containing an LA motif and an adjacent RNA recognition motif (RRM), and play diverse roles in RNA metabolism. Read More

    Effect of trichostatin A on Burkitt's lymphoma cells: Inhibition of EPS8 activity through Phospho-Erk1/2 pathway.
    Biochem Biophys Res Commun 2018 Feb 17. Epub 2018 Feb 17.
    Department of Hematology, Zhujiang Hospital, Southern Medical University, 510282, Guangzhou, China. Electronic address:
    Histone deacetylase inhibitors (HDACi) manifest great potential for treatment of Burkitt's lymphoma (BL), an aggressive B-cell lymphoma. Epidermal growth factor receptor pathway substrate 8 (EPS8) is confirmed overexpressed and associated with poor prognosis in solid tumors and leukemia. However, EPS8 expression and the relationship between EPS8 and HDACi on BL remains obscure. Read More

    A potential substrate binding pocket of BdcA plays a critical role in NADPH recognition and biofilm dispersal.
    Biochem Biophys Res Commun 2018 Feb 17. Epub 2018 Feb 17.
    National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China. Electronic address:
    Biofilm dispersal is characterized by the cell detachment from biofilms and expected to provide novel "anti-biofilm" approaches of prevention and treatment of biofilms in clinical and industrial settings. The E.coli protein BdcA has been identified as a biofilm dispersal factor and designed to be an important component in engineered applications to control biofilm formation. Read More

    Ubiquitin C-Terminal Hydrolase L1 regulates autophagy by inhibiting autophagosome formation through its deubiquitinating enzyme activity.
    Biochem Biophys Res Commun 2018 Feb 17. Epub 2018 Feb 17.
    School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China; Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address:
    Ubiquitination modification has been shown to play a key role in autophagy. Increasing studies reported the involvement of de-ubiquitinating enzymes (DUBs) in autophagy pathway. To systematically search how DUBs manipulate autophagy, we utilized a double fluorescence tagged LC3 stable HeLa cell line, and did a genome wide screen of 55 human DUBs which is about 60% coverage of the DUB family. Read More

    TGF-β1/TβRII/Smad3 signaling pathway promotes VEGF expression in oral squamous cell carcinoma tumor-associated macrophages.
    Biochem Biophys Res Commun 2018 Feb 17. Epub 2018 Feb 17.
    Department of Head and Neck Oncology, State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan Province 610041, China. Electronic address:
    Oral squamous cell carcinoma (OSCC) is the most common type of malignant cancer affecting the oral cavity. Tumor associated macrophages (TAMs) play a vital role in the initiation, progression and metastasis of OSCC. In this study, we investigated the correlation between macrophages and several clinical and pathological indicators, and we also explored how transforming growth factor-β1 (TGF-β1) effect on VEGF expression in TAMs. Read More

    Chlamydia pneumoniae infection promotes monocyte transendothelial migration by increasing vascular endothelial cell permeability via the tyrosine phosphorylation of VE-cadherin.
    Biochem Biophys Res Commun 2018 Feb 17. Epub 2018 Feb 17.
    Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Tianjin Medical University, No.22 Qixiangtai Road, Heping District, Tianjin, 300070, China. Electronic address:
    Migration of monocytes into the subendothelial layer of the intima is one of the critical events in early atherosclerosis. Chlamydia pneumoniae (C. pneumoniae) infection has been shown to promote monocyte transendothelial migration (TEM). Read More

    N-terminus plus linker domain of Mg-chelatase D subunit is essential for Mg-chelatase activity in Oryza sativa.
    Biochem Biophys Res Commun 2018 Feb 17. Epub 2018 Feb 17.
    Department of Horticulture, College of Agronomy, Jiangxi Agricultural University, Nanchang, 330045, PR China.
    Mg chelatase, a key enzyme in chlorophyll biosynthesis, is comprised of I, D and H subunits. Among these subunits, the D subunit was regarded to mediate protein interactions due to its unique protein domains. However, the functional roles of the different domains of the D subunit in vivo remain unclear. Read More

    MiR-29 family members interact with SPARC to regulate glucose metabolism.
    Biochem Biophys Res Commun 2018 Feb 17. Epub 2018 Feb 17.
    Department of Endocrinology and Metabolism, The Second Affiliated Hospital of Harbin Medical University, Hormone and Endocrinology Key Laboratory of Harbin Medical University, Xuefu Road 246, Harbin, 150080, China. Electronic address:
    MicroRNA (miR)-29 family members have been reported to play important regulatory roles in metabolic disease. We used TargetScan to show that "secreted protein acidic rich in cysteine" (SPARC) is a target of the miR-29s. SPARC is a multifunctional secretory protein involved in a variety of biological activities, and SPARC dysregulation is associated with a wide range of obesity-related disorders, including type 2 diabetes mellitus (T2DM). Read More

    Knockdown of fascin-1 expression suppresses cell migration and invasion of non-small cell lung cancer by regulating the MAPK pathway.
    Biochem Biophys Res Commun 2018 Feb 16. Epub 2018 Feb 16.
    Department of Oncology, The First Hospital of Lanzhou University, Lanzhou 730000, Gansu Province, People's Republic of China.
    Fascin-1 is a cytoskeletal protein and it can specifically bind to F-actin, it can be abnormally expressed in a variety of solid tumors. Fascin-1 was identified as a factor for poor prognosis in non-small cell lung cancer (NSCLC). However, the relevant molecular mechanisms are not yet fully understood. Read More

    Cell model for the identification and characterization of prion-like components from Alzheimer brain tissue.
    Biochem Biophys Res Commun 2018 Feb 16. Epub 2018 Feb 16.
    Institute of Protein Biochemistry, Ulm University, Helmholtzstr. 8/1, 89081 Ulm, Germany. Electronic address:
    Intracerebral injection of brain extracts from Alzheimer's disease (AD) patients into appropriate mouse models was previously found to drastically accelerate the deposition of Aβ amyloid in the recipient animals indicating a prion-like activity. In this study we show that this prion-like activity can be also identified by using a cell culture model of Aβ plaque formation. Analysis of biochemical fractions of AD brain extract indicate that the seeding-activity correlated with the presence of Aβ peptide and Aβ-derived aggregates. Read More

    Spinal cord-specific deletion of the glutamate transporter GLT1 causes motor neuron death in mice.
    Biochem Biophys Res Commun 2018 Feb 16. Epub 2018 Feb 16.
    Laboratory of Molecular Neuroscience, Medical Research Institute (MRI), Tokyo Medical and Dental University (TMDU), 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, Japan; Center for Brain Integration Research (CBIR), TMDU, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, Japan. Electronic address:
    Amyotrophic lateral sclerosis (ALS) is a chronic neurodegenerative disorder characterized by the selective loss of motor neurons. The precise mechanisms that cause the selective death of motor neurons remain unclear, but a growing body of evidence suggests that glutamate-mediated excitotoxicity has been considered to play an important role in the mechanisms of motor neuron degeneration in ALS. Reductions in glutamate transporter GLT1 have been reported in animal models of ALS and the motor cortex and spinal cord of ALS patients. Read More

    Structural characterization of the HCoV-229E fusion core.
    Biochem Biophys Res Commun 2018 Feb 16. Epub 2018 Feb 16.
    College of Life Sciences, College of Pharmacy, State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin, 300071, People's Republic of China.
    HCoV-229E spike (S) protein mediates virion attachment to cells and subsequent fusion of the viral and cellular membranes. This protein is composed of an N-terminal receptor-binding domain (S1) and a C-terminal trans-membrane fusion domain (S2). S2 contains a highly conserved heptad repeat 1 and 2 (HR1 and HR2). Read More

    Generation of anti-oligosaccharide antibodies that recognize mammalian glycoproteins by immunization with a novel artificial glycosphingolipid.
    Biochem Biophys Res Commun 2018 Feb 16. Epub 2018 Feb 16.
    Molecular and Biotechnology Research Group, Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan.
    Here we report a new method for the efficient generation of antibodies that recognize the fine structures of oligosaccharides on glycoproteins. We found a newly designed artificial glycosphingolipid carrying a very long chain fatty acid to be a strong immunogen in mice, with the serum of immunized mice containing antibodies recognizing the oligosaccharide structure of the immunogen. First, we found that conjugation of a simple ceramide analogue to target oligosaccharides could enhance the immunogenicity of these oligosaccharides in these immunized mice. Read More

    Increased mortality from influenza infection in long-chain acyl-CoA dehydrogenase knockout mice.
    Biochem Biophys Res Commun 2018 Feb 16. Epub 2018 Feb 16.
    Department of Pediatrics, Children's Hospital of Pittsburgh of UPMC, Pittsburgh, PA 15224, United States. Electronic address:
    We previously showed that the mitochondrial fatty acid oxidation enzyme long-chain acyl-CoA dehydrogenase (LCAD) is expressed in alveolar type II pneumocytes and that LCAD-/- mice have altered breathing mechanics and surfactant defects. Here, we hypothesized that LCAD-/- mice would be susceptible to influenza infection. Indeed, LCAD-/- mice demonstrated increased mortality following infection with 2009 pandemic influenza (A/CA/07/09). Read More

    CircRNA circ_0067934 promotes tumor growth and metastasis in hepatocellular carcinoma through regulation of miR-1324/FZD5/Wnt/β-catenin axis.
    Biochem Biophys Res Commun 2018 Feb 16. Epub 2018 Feb 16.
    Department of General Surgical, The Second People's Hospital of Shenzhen, Shenzhen 518110, China. Electronic address:
    Recently, increasing evidences demonstrate that circular RNAs (circRNAs) exert very important functions in the progression of human cancers. However, the functions and molecular mechanism of circ_0067934 in hepatocellular carcinoma (HCC) are largely unknown. In the present study, we found that the expression of circ_0067934 was significantly upregulated in HCC tissues and cell lines compared to adjacent normal tissues. Read More

    Overexpression of PtABCC1 contributes to mercury tolerance and accumulation in Arabidopsis and poplar.
    Biochem Biophys Res Commun 2018 Feb 15. Epub 2018 Feb 15.
    Key Laboratory of Eco-Environments in Three Gorges Reservoir Region (Ministry of Education), College of Life Sciences, Southwest University, Chongqing 400715, China. Electronic address:
    Mercury (Hg) is a highly biotoxic heavy metal that contaminates the environment. Phytoremediation is a green technology for environmental remediation and is used to clean up Hg contaminated soil in recent years. In this study, we isolated an ATP-binding cassette (ABC) transporter gene PtABCC1 from Populus trichocarpa and overexpressed it in Arabidopsis and poplar. Read More

    Increased cerebrospinal fluid complement C5 levels in major depressive disorder and schizophrenia.
    Biochem Biophys Res Commun 2018 Feb 16. Epub 2018 Feb 16.
    Department of Mental Disorder Research, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Tokyo, Japan. Electronic address:
    Inflammation has been implicated in a variety of psychiatric disorders. We aimed to determine whether levels of complement C5 protein in the cerebrospinal fluid (CSF), which may reflect activation of the complement system in the brain, are altered in patients with major psychiatric disorders. Additionally, we examined possible associations of CSF C5 levels with clinical variables. Read More

    JIP3 knockout protects mice against high fat diet-induced liver injury.
    Biochem Biophys Res Commun 2018 Feb 15. Epub 2018 Feb 15.
    Department of Endocrinology, The First Affiliated Hospital of Zhengzhou University, 1 Jianshe Rd., Zhengzhou 450052, China. Electronic address:
    Multiple pathways contribute to nonalcoholic fatty liver disease (NAFLD) in response to high fat diets (HFD). A homolog of mammalian JNK-interacting protein 3 (JIP3), also known as JSAP-1, activates different components in various signaling pathways to modulate cellular processes. The purpose of this study was to examine the role of JIP3 in obesity-related pathologies pathway. Read More

    Ess2 bridges transcriptional regulators and spliceosomal complexes via distinct interacting domains.
    Biochem Biophys Res Commun 2018 Feb 15. Epub 2018 Feb 15.
    Division of Biochemistry, Department of Biomedical Sciences, School of Medicine, Nihon University, Itabashi-ku, Tokyo 173-8610, Japan. Electronic address:
    Transcription and pre-mRNA splicing are complex, coupled processes that involve transcriptional co-regulators. Ess2 (also termed Dgcr14) is a nuclear protein that enhances the transcriptional activity of retinoic acid receptor-related orphan receptor gamma/gamma-t (Rorγ/γt). Ess2 is also a component of the spliceosomal C complex (containing U2, U5 and U6 snRNAs). Read More

    SARM1 deletion restrains NAFLD induced by high fat diet (HFD) through reducing inflammation, oxidative stress and lipid accumulation.
    Biochem Biophys Res Commun 2018 Feb 15. Epub 2018 Feb 15.
    Intensive Care Unit, Huai'an First People's Hospital, Nanjing Medical University, 6 Beijing Road West, Huai'an, Jiangsu 223300, PR China. Electronic address:
    SARM1 (Sterile alpha and armadillo motif-containing protein 1) is the recently identified TIR domain-containing cytosolic protein, which is involved in toll-like receptors (TLRs) signaling transduction. In the present study, the role of SARM1 in high fat diet (HFD)-induced non-alcoholic fatty liver disease (NAFLD) progression was explored. We found that SARM1 was expressed highly in fatty liver. Read More

    Effects of PQ's cytotoxicity on secretory vesicles in astroglia: Expression alternation of secretogranin II and its potential interaction with intracellular factors.
    Biochem Biophys Res Commun 2018 Feb 16. Epub 2018 Feb 16.
    Department of Forensic Genetics and Biology, School of Forensic Medicine, China Medical University, Shenyang, PR China. Electronic address:
    It has been extensively characterized that paraquat (PQ) selectively targets to the substantia nigra and exerts neurotoxic actions on dopaminergic neurons. However, a little knowledge is available about astroglia in PQ exposure, especially its complex secretory machinery. To explore this point, we built up a PQ-induced model in cultural U118 astrocyte. Read More

    Detection of synchronized burst firing in cultured human induced pluripotent stem cell-derived neurons using a 4-step method.
    Biochem Biophys Res Commun 2018 Feb 16. Epub 2018 Feb 16.
    Department of Electronics, Graduate School of Engineering, Tohoku Institute of Technology, 35-1 Yagiyama Kasumicho, Taihaku-ku, Sendai, Miyagi, 982-8577, Japan; iPS-non Clinical Experiments for Nervous System (iNCENS) Project, Japan; Consortium for Safety Assessment Using Human iPS Cells (CSAHi), Japan. Electronic address:
    Human induced pluripotent stem cell-derived neurons are promising for use in toxicity evaluations in nonclinical studies. The multi-electrode array (MEA) assay is used in such evaluation systems because it can measure the electrophysiological function of a neural network noninvasively and with high throughput. Synchronized burst firing (SBF) is the main analytic parameter of pharmacological effects in MEA data, but an accurate method for detecting SBFs has not been established. Read More

    NEK3-mediated SNAP29 phosphorylation modulates its membrane association and SNARE fusion dependent processes.
    Biochem Biophys Res Commun 2018 Feb 15. Epub 2018 Feb 15.
    School of Molecular Cell Biology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv, 6997801, Israel. Electronic address:
    Intracellular membrane fusion depends on the presence of specific mediators, the vesicle (v-) and the target (t-) SNAREs (Soluble N-ethylmaleimide-sensitive factor, NSF, attachment protein SNAP receptors), whose interaction brings apposing membranes to close proximity and initiates their fusion. SNAP29 (synaptosomal-associated protein 29), a t-SNARE protein, is involved in multiple fusion events during intracellular transport and affects structure of organelles such as the Golgi apparatus and focal adhesions. Mutations in SNAP29 gene result in CEDNIK (Cerebral dysgenesis, neuropathy, ichthyosis and palmoplantar keratoderma) syndrome. Read More

    Adenosine receptors enhance the ATP-induced odontoblastic differentiation of human dental pulp cells.
    Biochem Biophys Res Commun 2018 Feb 15. Epub 2018 Feb 15.
    Department of Prosthodontics, Peking University School and Hospital of Stomatology, Beijing, China; Center for Oral Functional Diagnosis, Treatment and Research, Peking University School and Hospital of Stomatology, China. Electronic address:
    Purinergic signaling regulates various biological processes through the activation of adenosine receptors (ARs) and P2 receptors. ATP induces the odontoblastic differentiation of human dental pulp cells (HDPCs) via P2 receptors. However, there is no information available about the roles of ARs in HDPC odontoblastic differentiation induced by ATP. Read More

    The high bone mass phenotype of Lrp5-mutant mice is not affected by megakaryocyte depletion.
    Biochem Biophys Res Commun 2018 Feb 15. Epub 2018 Feb 15.
    Department of Osteology and Biomechanics, University Medical Center Hamburg Eppendorf, Hamburg 20246, Germany. Electronic address:
    Bone remodeling is a continuously ongoing process mediated by bone-resorbing osteoclasts and bone-forming osteoblasts. One key regulator of bone formation is the putative Wnt co-receptor Lrp5, where activating mutations in the extracellular domain cause increased bone formation in mice and humans. We have previously reported that megakaryocyte numbers are increased the bone marrow of mice carrying a high bone mass mutation (HBM) of Lrp5 (Lrp5). Read More

    First-line anti-tuberculosis drugs induce hepatotoxicity: A novel mechanism based on a urinary metabolomics platform.
    Biochem Biophys Res Commun 2018 Feb 15. Epub 2018 Feb 15.
    Department of Clinical Pharmacology, The First Affiliated Hospital of Soochow University, Suzhou 215006, China; College of Pharmaceutical Science, Soochow University, Suzhou 215006, China. Electronic address:
    Tuberculosis (TB) has become a global public health and social threat. As clinical first-line drugs, rifampicin and isoniazid used in combination with pyrazinamide and ethambutol (the HRZE regimen) usually induce hepatotoxicity. However, the mechanisms underlying this phenomenon remain unclear, and studying the metabolic impact of co-treating TB patients with the HRZE regimen can provide new hepatotoxicity evidence. Read More

    The role of Foxq1 in proliferation of human dental pulp stem cell.
    Biochem Biophys Res Commun 2018 Feb 14. Epub 2018 Feb 14.
    Guanghua School of Stomatology, Hospital of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, 56 Lingyuanxi Road, Guangzhou, Guangdong, 510055, China. Electronic address:
    This study aimed to investigate the role for Foxq1 in proliferation activity regulation of dental pulp stem cells (DPSCs). Proliferation of DPSC was induced by calcium hydroxide, then expression alteration of Foxq1 was evaluated. Lentivirus was employed to manipulate Foxq1 level in DPSC, and proliferation activities were evaluated. Read More

    Sterol O-acyltransferase 1 deficiency improves defective insulin signaling in the brains of mice fed a high-fat diet.
    Biochem Biophys Res Commun 2018 Feb 14. Epub 2018 Feb 14.
    Department of Neurosurgery, The First Hospital of Jilin University, 71 Xinmin Street, Changchun, 130021, China. Electronic address:
    Insulin resistance induced by a high-fat diet (HFD) is related to metabolic diseases, and sterol O-acyltransferase 1 (SOAT1) is a key enzyme for the biosynthesis of cholesteryl ester. In the present study, wild-type (WT) mice and SOAT1-knockout (KO) mice with a C57BL6 background fed a HFD were used to explore the role of SOAT1 in the hypothalamus. The results show that the WT mice exhibited a significant increase in body weight as well as hepatic histologic changes; they also had a lower glucose and insulin tolerance than the WT mice fed a normal diet. Read More

    Zika virus infected primary microglia impairs NPCs proliferation and differentiation.
    Biochem Biophys Res Commun 2018 Feb 14. Epub 2018 Feb 14.
    State Key Laboratory of Pharmaceutical Biotechnology, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, Nanjing Advanced Institute for Life Sciences (NAILS), School of Life Sciences, Nanjing University, Nanjing, Jiangsu, 210046, China. Electronic address:
    Zika virus (ZIKV) can lead to severe birth defects especially microcephaly in newborns by infecting human neural progenitors and impairing brain development. However, as the resident immune cells in the brain, the role of microglia in the ZIKV pathology is not clearly defined. To understand the interplay between immune response and neural cells, we investigate the interaction between microglia and NPCs during ZIKV infection. Read More

    GATA3 acetylation at K119 by CBP inhibits cell migration and invasion in lung adenocarcinoma.
    Biochem Biophys Res Commun 2018 Feb 14. Epub 2018 Feb 14.
    Department of Anatomy, Histology and Embryology, MOE Key Laboratory of Carcinogenesis and Translational Research, State Key Laboratory of Natural and Biomimetic Drugs, Peking University Health Science Center, Beijing, 100191, China. Electronic address:
    GATA3 is a transcriptional factor involved in the development of multiple organs. Post translational modifications of GATA3 are critical to its function. Here, we report that GATA3 interacts with and is acetylated by the acetyltransferase CBP. Read More

    Aldolase A overexpression is associated with poor prognosis and promotes tumor progression by the epithelial-mesenchymal transition in colon cancer.
    Biochem Biophys Res Commun 2018 Feb 14. Epub 2018 Feb 14.
    Xiamen Diabetes Institution, The First Affiliated Hospital of Xiamen University, Xiamen, China. Electronic address:
    There is increasing evidence that glycolysis is involved in cancer progression. Aldolase is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde-3-phosphate and dihydroxyacetone phosphate. Disruption of the aldolase genes also plays a role in the progression of multiple types of cancer. Read More

    Glucagon-like peptide-1 contributes to increases ABCA1 expression by downregulating miR-758 to regulate cholesterol homeostasis.
    Biochem Biophys Res Commun 2018 Feb 14. Epub 2018 Feb 14.
    Department of Hepatopancreatobiliary Surgery, Second Affiliated Hospital of Harbin Medical University, Harbin, 150086, China. Electronic address:
    Abnormal regulation of lipid metabolism is associated with type 2 diabetes mellitus (T2DM). GLP-1 as a new treatment for T2DM, has unique effects in modulating cholesterol homeostasis. However, the mechanism of this effect is largely missing. Read More

    The C2'- and C3'-endo equilibrium for AMP molecules bound in the cystathionine-beta-synthase domain.
    Biochem Biophys Res Commun 2018 Feb 14. Epub 2018 Feb 14.
    National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China; State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, No.1 Beichen West Road, Chaoyang District, Beijing 100101, China. Electronic address:
    The equilibrium between C2'- and C3'-endo conformations of nucleotides in solution, as well as their polymers DNA and RNA, has been well studied in previous work. However, this equilibrium of nucleotides in their binding state remains unclear. We observed two AMP molecules, in C3'- and C2'-endo conformations respectively, simultaneously bound to a cystathionine-beta-synthase (CBS) domain dimer of the magnesium and cobalt efflux protein CorC in the crystallographic study. Read More

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