88,431 results match your criteria Biochemical and biophysical research communications[Journal]


Neutralization of HSF1 in cells from PIK3CA-related overgrowth spectrum patients blocks abnormal proliferation.

Biochem Biophys Res Commun 2020 Jun 30. Epub 2020 Jun 30.

INSERM, LNC UMR1231 Team HSP-pathies, University of Burgundy and Franche-Comté, F-21000, Dijon, France. Electronic address:

PIK3CA-related overgrowth spectrum is caused by mosaicism mutations in the PIK3CA gene. These mutations, which are also observed in various types of cancer, lead to a constitutive activation of the PI3K/AKT/mTOR pathway, increasing cell proliferation. Heat shock transcription factor 1 (HSF1) is the major stress-responsive transcription factor. Read More

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http://dx.doi.org/10.1016/j.bbrc.2020.04.146DOI Listing

The temperature dependence of kinesin motor-protein mechanochemistry.

Biochem Biophys Res Commun 2020 Jun 30. Epub 2020 Jun 30.

Department of Physics and Astronomy and LaserLaB, Vrije Universiteit, 1081 HV, Amsterdam, the Netherlands. Electronic address:

Biophysical studies of the mechanochemical cycle of kinesin motors are essential for understanding the mechanism of energy conversion. Here, we report a systematic study of the impact of temperature on velocity and run length of homodimeric Drosophila kinesin-1, homodimeric C. elegans OSM-3 and heterodimeric C. Read More

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http://dx.doi.org/10.1016/j.bbrc.2020.06.004DOI Listing

G-quadruplexes: tools, roles, and goals.

Biochem Biophys Res Commun 2020 Jun 29. Epub 2020 Jun 29.

Center for Healthy Aging, Changzhi Medical College, Changzhi, 046000, Shanxi, PR China; State Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, PR China.

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http://dx.doi.org/10.1016/j.bbrc.2020.05.092DOI Listing

CXCR2 antagonist attenuates neutrophil transmigration into brain in a murine model of LPS induced neuroinflammation.

Biochem Biophys Res Commun 2020 Jun 29. Epub 2020 Jun 29.

Department of Immunology, School of Laboratory Medicine, Anhui Province Key Laboratory of Immunology in Chronic Diseases, Bengbu Medical College, Bengbu, Anhui, 233030, China. Electronic address:

Sepsis-associated encephalopathy (SAE) is a devastating neurological complication of sepsis with intolerable high motility. SAE is accompanied with brain vascular injury, endothelial hyperpermeability, and neutrophil infiltration into the brain tissue, key inflammatory processes leading to further brain edema and neuronal cell apoptosis. Recent studies from us and others suggest that the chemokine receptor C-X-C Motif Chemokine Receptor 2 (CXCR2) is crucial for neutrophil recruitment during SAE. Read More

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http://dx.doi.org/10.1016/j.bbrc.2020.05.124DOI Listing

CADM1 suppresses c-Src activation by binding with Cbp on membrane lipid rafts and intervenes colon carcinogenesis.

Biochem Biophys Res Commun 2020 Jun 29. Epub 2020 Jun 29.

Division of Molecular Pathology, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan. Electronic address:

Cell adhesion molecules act as tumor suppressors primarily by cell attachment activity, but additional mechanisms modifying signal transduction are suggested in some cases. Cell adhesion molecule 1 (CADM1), a membrane-spanning immunoglobulin superfamily, mediates intercellular adhesion by trans-homophilic interaction and acts as a tumor suppressor. Here, we investigated CADM1-associated proteins comprehensively using proteomic analysis of immune-precipitates of CADM1 by mass spectrometry and identified a transmembrane adaptor protein, Csk-binding protein (Cbp), known to suppress Src-mediated transformation, as a binding partner of CADM1. Read More

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http://dx.doi.org/10.1016/j.bbrc.2020.05.103DOI Listing

Dual phosphorylation of protein phosphatase PPM1H promotes dephosphorylation of Smad1 in cellulo.

Biochem Biophys Res Commun 2020 Jun 26. Epub 2020 Jun 26.

Department of Life Sciences, Faculty of Agriculture, Kagawa University, Kagawa, 761-0795, Japan. Electronic address:

Protein phosphatase PPM1H is known to participate in various biological or pathophysiological mechanisms. However, little is known about the molecular mechanisms of its regulation. In this study, we investigated the protein kinases that directly phosphorylate PPM1H, identifying them as cAMP-dependent protein kinase (PKA) and Ca/calmodulin-dependent protein kinase I (CaMKI). Read More

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http://dx.doi.org/10.1016/j.bbrc.2020.05.082DOI Listing

TSLP is a negative regulator of RANKL-induced osteoclastogenesis.

Biochem Biophys Res Commun 2020 Jun 26. Epub 2020 Jun 26.

Oral Health Science Center, Tokyo Dental College, Tokyo, 101-0061, Japan; Tokyo Dental College Research Branding Project, Tokyo Dental College, Tokyo, 101-0061, Japan; Department of Biochemistry, Tokyo Dental College, Tokyo, 101-0061, Japan.

Thymic stromal lymphopoietin (TSLP) is a member of the IL-2 cytokine family, which is known to activate type 2 innate lymphoid cells, mast cells, and Th2 cells; this activation results in allergic inflammation and host defense against parasites. TSLP has also been shown to promote Th17-mediated immune responses, such as those observed in the development of rheumatoid arthritis; however, its role in osteoclastogenesis remains poorly understood. Here, we investigated the functional involvement of TSLP in RANKL-induced osteoclast differentiation from murine bone marrow-derived macrophages (BMMs). Read More

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http://dx.doi.org/10.1016/j.bbrc.2020.05.055DOI Listing

EPS8 regulates an NLRP3 inflammasome-independent caspase-1 activation pathway in monosodium urate crystal-treated RAW264.7 macrophages.

Biochem Biophys Res Commun 2020 Jun 25. Epub 2020 Jun 25.

Department of Pharmacology, College of Medicine, National Cheng Kung University, Tainan, 70101, Taiwan, ROC; Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan, 70101, Taiwan, ROC. Electronic address:

Gout is an inflammatory arthritis caused by the phagocytosis of monosodium urate (MSU) crystal deposition in joints. NOD-, LRR-, and pyrin domain-containing 3 (NLRP3) inflammasome-dependent caspase-1 activation is implicated in the processing of interleukin-1β (IL-1β), which is the major effector cytokine in the acute inflammatory response of gout. Mechanisms underlying caspase-1 activation remain unclear. Read More

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http://dx.doi.org/10.1016/j.bbrc.2020.05.084DOI Listing

Brown adipocyte-derived exosomal miR-132-3p suppress hepatic Srebf1 expression and thereby attenuate expression of lipogenic genes.

Biochem Biophys Res Commun 2020 Jun 25. Epub 2020 Jun 25.

Department of Medical Biochemistry, Faculty of Life Sciences, Kumamoto University, Kumamoto, 860-8556, Japan; Center for Metabolic Regulation of Healthy Aging (CMHA), Faculty of Life Sciences, Kumamoto University, Kumamoto, 860-8556, Japan. Electronic address:

Recent evidence has revealed a novel signaling mechanism through which brown adipose tissue (BAT)-derived exosomal microRNAs (miRNAs) influence hepatic gene expression. Here, we uncover neuronal control of these miRNAs and identify exosomal miR-132-3p as a regulator of hepatic lipogenesis under cold stress conditions. Norepinephrine, a sympathetic nervous system neurotransmitter mediating cold-induced BAT activation, altered the composition of brown adipocyte (BAC)-derived exosomal miRNAs; among them, miR-132-3p was significantly induced. Read More

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http://dx.doi.org/10.1016/j.bbrc.2020.05.090DOI Listing

Ursodeoxycholic acid alleviates nonalcoholic fatty liver disease by inhibiting apoptosis and improving autophagy via activating AMPK.

Biochem Biophys Res Commun 2020 Jun 25. Epub 2020 Jun 25.

Medical examination center, Renmin Hospital of Wuhan University, Wuhan, Hubei Province, 430061, China. Electronic address:

Ursodeoxycholic acid (UDCA), first identified in bear bile, was widely used in cholestatic liver diseases. Our previous studies have suggested UDCA may exert favorable influence on hepatic steatosis. However, the molecular mechanism remains elusive. Read More

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http://dx.doi.org/10.1016/j.bbrc.2020.05.128DOI Listing

Monensin inhibits glioblastoma angiogenesis via targeting multiple growth factor receptor signaling.

Biochem Biophys Res Commun 2020 Jun 25. Epub 2020 Jun 25.

Department of Neurosurgery, Affiliated Hospital of Southwest Medical University, China; Neurosurgery Clinical Medical Research Center of Sichuan Province, China; Academician (Expert) Workstation of Sichuan Province, China. Electronic address:

Glioblastoma is characterized by the extensive vascularization with poor prognosis. Targeting both tumor cell and angiogenesis may present an effective therapeutic strategy for glioblastoma. Monensin, a polyether ionophore antibiotic, has been recently recognized as promising anticancer drug candidate due to its potent and selective anti-tumor activities. Read More

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http://dx.doi.org/10.1016/j.bbrc.2020.05.057DOI Listing

The GLP-1 receptor herbal agonist morroniside attenuates neuropathic pain via spinal microglial expression of IL-10 and β-endorphin.

Biochem Biophys Res Commun 2020 Jun 25. Epub 2020 Jun 25.

King's Lab, Shanghai Jiao Tong University School of Pharmacy, Shanghai, 200240, China. Electronic address:

Objectives: To assess the protective effect of the glucagon-like peptide-1 receptor (GLP-1R) agonist morroniside against neuropathic pain and its downstream mechanisms of activating microglial GLP-1R/interleukin-10 (IL-10)/β-endorphin antinociceptive pathway.

Methods: Spinal nerve ligation-induced neuropathic pain rats were intrathecally injected with morroniside, with mechanical paw withdrawal threshold being assessed. The expression of spinal and cultured microglia IL-10 and β-endorphin were detected with qRT-PCR. Read More

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http://dx.doi.org/10.1016/j.bbrc.2020.05.080DOI Listing

Generation and characterization of dromedary Tenascin-C and Tenascin-W specific antibodies.

Biochem Biophys Res Commun 2020 Jun 24. Epub 2020 Jun 24.

Laboratoire des Venins et Molécules Thérapeutiques, Institut Pasteur Tunis, 13 Place Pasteur, BP74, 1002, Tunis- Université Tunis El Manar Tunisia, Tunisia; Faculté de Médecine de Tunis, 15 Rue Djebel Lakhdhar, La Rabta, 1007, Tunis-Université Tunis el Manar, Tunisia. Electronic address:

Tenascin-C (TNC) and tenascin-W (TNW), large hexameric glycoproteins overexpressed in the tumor microenvironment, are useful tumor biomarkers for theranostic applications. For now, polyclonal and monoclonal antibodies, as well as aptamers targeting TNC and TNW have been developed. However, the immunostaining sensitivity of antibodies is very heterogenous. Read More

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http://dx.doi.org/10.1016/j.bbrc.2020.05.077DOI Listing

Detection of cellular G-quadruplex by using a loop structure as a structural determinant.

Biochem Biophys Res Commun 2020 Jun 22. Epub 2020 Jun 22.

Department of Basic Medical Sciences, Tokyo Metropolitan Institute of Medical Science, Kamikitazawa, Setagaya-ku, Tokyo, 156-8506, Japan.

G-quadrupex is now known to play crucial roles in various biological reactions. However, direct evidence for its presence in cells has been limited, due to the lack of versatile and non-biased methodology. We use Rif1 binding sites on the fission yeast genome, which has been shown to adopt G4 structures, as a model to prove that Rif1 BS indeed adopt G4 structure in cells. Read More

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http://dx.doi.org/10.1016/j.bbrc.2020.05.191DOI Listing

Corrigendum to "A novel Kunitz-type neurotoxin peptide identified from skin secretions of the frog Amolops loloensis" [Biochem. Biophys. Res. Commun. 528 (1) (2020) 99-104].

Biochem Biophys Res Commun 2020 Jun 20. Epub 2020 Jun 20.

School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou, 325035, Zhejiang, China. Electronic address:

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http://dx.doi.org/10.1016/j.bbrc.2020.06.035DOI Listing

rDNA subtypes and their transcriptional expression in zebrafish at different developmental stages.

Biochem Biophys Res Commun 2020 Jun 19. Epub 2020 Jun 19.

MOE Key Laboratory for Molecular Animal Nutrition, College of Animal Sciences, Zhejiang University, Hangzhou, 310058, China. Electronic address:

Eukaryotic 18S, 5.8S and 28S rRNAs are processed from a single transcript transcribed from the 45S rDNA gene, which is normally tandemly arrayed over hundred copies in a genome. Recently, a maternal (M) subtype and a somatic (S) subtype of rDNA were identified in zebrafish, with M-subtype on chromosome 4 and S-subtype on chromosome 5. Read More

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http://dx.doi.org/10.1016/j.bbrc.2020.05.196DOI Listing

Development, establishment and validation of in vitro and ex vivo assays of vascular calcification.

Biochem Biophys Res Commun 2020 Jun 16. Epub 2020 Jun 16.

Institute for Molecular Cardiovascular Research (IMCAR), RWTH Aachen University, University Hospital, Aachen, Germany.

Objective: Vascular calcification (VC) is one major complication in patients with chronic kidney disease, with a misbalance in calcium and phosphate metabolism playing crucial role. The mechanisms underlying VC have not been entirely revealed to date. As studies aiming at the identification and characterization of the involved mediators are highly relevant, we developed a standardized operating protocol for in vitro and ex vivo approaches in this study to aiming at the comparability of these studies. Read More

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http://dx.doi.org/10.1016/j.bbrc.2020.05.085DOI Listing

Rac1-dependent endocytosis and Rab5-dependent intracellular trafficking are required by Enterovirus A71 and Coxsackievirus A10 to establish infections.

Biochem Biophys Res Commun 2020 Aug 6;529(1):97-103. Epub 2020 Jun 6.

Shanghai Public Health Clinical Center & Institutes of Biomedical Sciences, Fudan University, Shanghai, China. Electronic address:

Enterovirus A71 (EVA71) and Coxsackievirus A10 (CVA10) are representative types of Enterovirus A. Dependent on the host cell types, the EVA71 entry may utilize clathrin-, caveola-, and endophilin-A2-mediated endocytosis. However, the cell-entry and intracellular trafficking pathways of CVA10, using KREMEN1 as its receptor, are unclear. Read More

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http://dx.doi.org/10.1016/j.bbrc.2020.05.058DOI Listing

Obtusifoliol 14α-demethylase OsCYP51G1 is involved in phytosterol synthesis and affects pollen and seed development.

Biochem Biophys Res Commun 2020 Aug 6;529(1):91-96. Epub 2020 Jun 6.

Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement, Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, China; Center of Economic Botany, Core Botanical Gardens, Chinese Academy of Sciences, Guangzhou, China. Electronic address:

As structural components of biological membranes, phytosterols are essential not only for a variety of cellular functions but are also precursors for brassinosteroid (BR) biosynthesis. Plant CYP51 is the oldest and most conserved obtusifoliol 14α-demethylase in eukaryotes and is an essential component of the sterol biosynthesis pathway. However, little is known about rice (Oryza sativa L. Read More

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http://dx.doi.org/10.1016/j.bbrc.2020.05.216DOI Listing

TRPC1 exacerbate metastasis in gastric cancer via ciRS-7/miR-135a-5p/TRPC1 axis.

Biochem Biophys Res Commun 2020 Aug 6;529(1):85-90. Epub 2020 Jun 6.

Department of Thoracic Surgery, First Affiliated Hospital of Xi'an Jiaotong University, Shaanxi, China. Electronic address:

Metastasis is frequently occurred in end-stage GC. Nevertheless, the initiation and progression of metastasis in GC remains unclear. The transient receptor potential canonical (TRPC) has been confirmed to be crucial for metastasis in many kinds of tumors, including GC. Read More

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http://dx.doi.org/10.1016/j.bbrc.2020.05.181DOI Listing

HCV-2a NS5A downregulates viral translation predominantly through domain I.

Biochem Biophys Res Commun 2020 Aug 5;529(1):77-84. Epub 2020 Jun 5.

Vaccine and Infectious Disease Organization-International Vaccine Centre, University of Saskatchewan, Saskatoon, Saskatchewan, Canada; Vaccinology and Immunotherapeutics, School of Public Health, University of Saskatchewan, Saskatoon, Saskatchewan, Canada; Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada. Electronic address:

Hepatitis C virus (HCV) non-structural protein NS5A is a multifunctional protein with critical roles in viral replication and assembly. We previously showed that HCV-1b NS5A downregulates viral translation only in the presence of the poly(U/UC) tract in 3'UTR. As NS5A of different HCV genotypes may have different functions or carry out the same functions through genotype-specific mechanisms, we investigated the effect of HCV-2a NS5A on viral translation. Read More

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http://dx.doi.org/10.1016/j.bbrc.2020.05.177DOI Listing

Transcriptional regulation of Salmochelin glucosyltransferase by Fur in Salmonella.

Biochem Biophys Res Commun 2020 Aug 5;529(1):70-76. Epub 2020 Jun 5.

Department of Microbiology, School of Medicine, Kyungpook National University, 680 Gukchaebosang-Ro, Jung-gu, Daegu, 41944, South Korea. Electronic address:

Pathogenic bacteria acquire the acquisition of iron from the host to ensure their survival. Salmonella spp. utilizes siderophores, including salmochelin, for high affinity aggressive import of iron. Read More

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http://dx.doi.org/10.1016/j.bbrc.2020.06.009DOI Listing

Topical GDF11 accelerates skin wound healing in both type 1 and 2 diabetic mouse models.

Biochem Biophys Res Commun 2020 Aug 5;529(1):7-14. Epub 2020 Jun 5.

Department of Pharmacology (State-Province Key Laboratories of Biomedicine-Pharmaceutics of China, Key Laboratory of Cardiovascular Medicine Research, Ministry of Education), College of Pharmacy, Harbin Medical University, Harbin, Heilongjiang, 150081, PR China. Electronic address:

This study aimed to investigate the role of truncated growth differentiation factor 11 (GDF11), in which the recognition site of Furin from wild-type GDF11 was deleted to enhance the cellular stability, in skin wound healing in the setting of diabetes mellitus (DM) and the underlying mechanisms. Our study found that both truncated and natural GDF11s effectively accelerated wound healing processes in both T1DM and T2DM mice with a potency compatible to PDGF, bFGF, and EGF, but being much higher than GDF8. At the cellular level, GDF11 stimulated the proliferation and suppressed HG-induced apoptosis of HSFs. Read More

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http://dx.doi.org/10.1016/j.bbrc.2020.05.036DOI Listing

Intestinal microbiota transplantation reveals the role of microbiota in dietary regulation of RegIIIβ and RegIIIγ expression in mouse intestine.

Biochem Biophys Res Commun 2020 Aug 5;529(1):64-69. Epub 2020 Jun 5.

Research Faculty of Agriculture, Hokkaido University, Sapporo, 060-8589, Japan. Electronic address:

RegIIIβ and RegIIIγ are antimicrobial peptides expressed in intestinal epithelial cells. Expression of these peptides is reportedly decreased by high-fat diet (HFD) and increased by indigestible oligosaccharides in mice. Clearly, these dietary regimens change the structure of intestinal microbiota. Read More

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http://dx.doi.org/10.1016/j.bbrc.2020.05.150DOI Listing

A simple bacterial expression system for human ppGalNAc-T and used for the synthesis of O-GalNAc glycosylated interleukin 2.

Biochem Biophys Res Commun 2020 Aug 5;529(1):57-63. Epub 2020 Jun 5.

Key Laboratory of Systems Biomedicine (Ministry of Education) and Collaborative Innovation Center of Systems Biomedicine, Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, China. Electronic address:

Mucin-type O-glycosylation (hereafter referred to as O-GalNAc glycosylation) is one of the most abundant glycosylation on proteins. It is initiated by the members of polypeptide N-acetyl-α-galactosaminyltransferases (ppGalNAc-Ts) family. The ppGalNAc-Ts could be used as tool enzymes to modify target proteins including therapeutic glycoprotein drugs with O-GalNAc glycosylation at specific glycosylated sites in vitro. Read More

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http://dx.doi.org/10.1016/j.bbrc.2020.05.209DOI Listing

Prolonged siRNA expression in mammalian cells using an Epstein-Barr virus-based plasmid expression system.

Biochem Biophys Res Commun 2020 Aug 5;529(1):51-56. Epub 2020 Jun 5.

State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, 210023, People's Republic of China. Electronic address:

RNA interference (RNAi) is a powerful tool in gene function analysis and disease treatment, especially diseases that are 'undruggable' by classical small molecules. However, the RNAi applications are limited due to some defects, such as short duration and toxic side effects. New strategies are still needed to improve RNAi applications. Read More

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http://dx.doi.org/10.1016/j.bbrc.2020.05.219DOI Listing

Ubiquitination of IGF2BP3 by E3 ligase MKRN2 regulates the proliferation and migration of human neuroblastoma SHSY5Y cells.

Biochem Biophys Res Commun 2020 Aug 5;529(1):43-50. Epub 2020 Jun 5.

Department of Anesthesiology, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325000, Zhejiang, China. Electronic address:

Neuroblastoma (NB) is a paediatric tumour that shows great biomolecule and clinical heterogeneity, and patients with NB often develop various neurological complications. Currently, the disease is mainly treated by surgery and still lacks specific therapeutic drugs; therefore, targets are urgently needed. Makorin ring finger protein 2 (MKRN2) is an E3 ligase whose effects on neuroblastoma have not been illustrated. Read More

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http://dx.doi.org/10.1016/j.bbrc.2020.05.112DOI Listing

Osteoclastic miR-301-b knockout reduces ovariectomy (OVX)-induced bone loss by regulating CYDR/NF-κB signaling pathway.

Biochem Biophys Res Commun 2020 Aug 5;529(1):35-42. Epub 2020 Jun 5.

Department of Orthopedics, Zhejiang Hospital, Hangzhou City, Zhejiang Province, 310030, China. Electronic address:

Postmenopausal osteoporosis (PMOP) is a frequent bone disorder responsible for an increased risk of disability to millions of individuals in the world. For identifying novel and effective targets to treat this disease, it is essential to explore the underlying molecular mechanisms. MicroRNAs (miRNAs) have been widely investigated due to their involvement in the pathophysiology of bone loss. Read More

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http://dx.doi.org/10.1016/j.bbrc.2020.05.111DOI Listing

TRIM59 attenuates IL-1β-driven cartilage matrix degradation in osteoarthritis via direct suppression of NF-κB and JAK2/STAT3 signaling pathway.

Biochem Biophys Res Commun 2020 Aug 5;529(1):28-34. Epub 2020 Jun 5.

Department of Orthopaedics Surgery, Punan Hospital of Pudong New District, Shanghai, 200125, China. Electronic address:

The tripartite motif (TRIM) protein family are implicated in a wide array of cellular processes, including cell growth, differentiation, apoptosis and inflammation. This study aimed to investigate the specific function of TRIM59 in chondrocytes and its association with the pathophysiology of osteoarthritis (OA). We observed the downregulated TRIM59 expression in OA cartilage compared to normal tissues. Read More

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http://dx.doi.org/10.1016/j.bbrc.2020.05.130DOI Listing

New imidazoles cause cellular toxicity by impairing redox balance, mitochondrial membrane potential, and modulation of HIF-1α expression.

Biochem Biophys Res Commun 2020 Aug 5;529(1):23-27. Epub 2020 Jun 5.

Department of Biochemistry, Medicinal Biochemistry, Nanomedicine & Toxicology Laboratory, Landmark University, PMB 1001, Omu-Aran, 251101, Nigeria.

Background: Our previous reports demonstrated the prospects of a new series of imidazoles as a source of alternative anti-parasite treatments, thus warranting further studies that include toxicity profiling.

Objective: In this study, we evaluated three imidazoles: bis-imidazole (compound 1), phenyl-substituted 1H-imidazole (compound 2), and thiopene-imidazole (compound 3) for cellular toxicity and possible mechanisms.

Methods: The three (3) compounds were assessed for in vitro cytotoxic action. Read More

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http://dx.doi.org/10.1016/j.bbrc.2020.05.059DOI Listing

Gomisin J inhibits the glioma progression by inducing apoptosis and reducing HKII-regulated glycolysis.

Biochem Biophys Res Commun 2020 Aug 5;529(1):15-22. Epub 2020 Jun 5.

Department of Neurosurgery, General Hospital of Tianjin Medical University, Tianjin, 300052, China. Electronic address:

Glioma is a leading cause of central nervous system malignant tumor-associated deaths in the world. However, the molecular mechanisms for glioma progression are still unclear, lacking effective therapeutic strategies. Gomisin J (GomJ) is a derivative of lignan compound, and shows regulatory effects on virus, oxidative stress and tumor progression. Read More

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http://dx.doi.org/10.1016/j.bbrc.2020.05.109DOI Listing

Regulation of IRS-1, insulin signaling and glucose uptake by miR-143/145 in vascular smooth muscle cells.

Biochem Biophys Res Commun 2020 Aug 8;529(1):119-125. Epub 2020 Jun 8.

Department of Experimental Medical Science, Lund University, Lund, Sweden. Electronic address:

Regulation of insulin signaling by microRNAs in smooth muscle cells may contribute to diabetic vascular disease. The two smooth muscle enriched miRNAs miR-143 and miR-145 have been reported to target mediators of insulin signaling in non-smooth muscle cells. In this study, we aimed to determine the importance of this regulation in vascular smooth muscle cells, where expression of miR-143/145 is much higher than in other cell types. Read More

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http://dx.doi.org/10.1016/j.bbrc.2020.05.148DOI Listing

Plakoglobin is involved in cytoskeletal rearrangement of podocytes under the regulation of UCH-L1.

Biochem Biophys Res Commun 2020 Aug 8;529(1):112-118. Epub 2020 Jun 8.

Department of Pathology, Shanghai Medical College, Fudan University, Shanghai, 200032, PR China. Electronic address:

UCH-L1 is a de-ubiquitination enzyme comprehensively distributed in neural cells and podocytes, which is involved in several kinds of nervous system and kidney related diseases. Our previous studies have demonstrated the aberrant up-regulation of UCH-L1 in podocytes of renal diseases, but how dose podocytes are injured by up-regulated UCH-L1 is waiting to be elucidated. Here, we observed the cytoskeleton rearrangement in podocytes with over-expression of UCH-L1, accompanied with a down-regulation of synaptopodin and RhoA, which are closely related to cytoskeletal stabilization. Read More

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http://dx.doi.org/10.1016/j.bbrc.2020.05.093DOI Listing

Effects of leptin on HPG axis and reproductive function in male rat in simulated altitude of 5500 m hypoxia environment.

Biochem Biophys Res Commun 2020 Aug 6;529(1):104-111. Epub 2020 Jun 6.

Institute of Basic Medical Sciences Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Beijing, 100005, China; Center of Environmental and Health Sciences, Chinese Academy of Medical Sciences, Beijing, China. Electronic address:

High altitude hypobaric hypoxia environment impairs male's reproductive function. Leptin is an adipose tissue-derived hormone which regulates body weight homeostasis. Its receptor (LepR) has been found in all levels of male reproductive axis, indicating that it can affect male reproductive system in a direct or (and) indirect way. Read More

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http://dx.doi.org/10.1016/j.bbrc.2020.05.194DOI Listing

Epilepsy- and intellectual disability-associated CYFIP2 interacts with both actin regulators and RNA-binding proteins in the neonatal mouse forebrain.

Biochem Biophys Res Commun 2020 Aug 5;529(1):1-6. Epub 2020 Jun 5.

Department of Neuroscience, College of Medicine, Korea University, Seoul, 02841, South Korea; Department of Biomedical Sciences, College of Medicine, Korea University, Seoul, 02841, South Korea. Electronic address:

Variants of the cytoplasmic FMR1-interacting protein 2 (CYFIP2) gene are associated with early-onset epileptic encephalopathy, intellectual disability, and developmental delay. However, the current understanding of the molecular functions of CYFIP2 is limited to those related to actin dynamics, and thus, the detailed mechanisms of CYFIP2-associated brain disorders remain largely unknown. Here, we isolated the neonatal forebrain CYFIP2 complex using newly generated Cyfip2-3×Flag knock-in mice, and performed mass spectrometry-based analyses to identify proteins in the complex. Read More

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http://dx.doi.org/10.1016/j.bbrc.2020.05.221DOI Listing

RNF115-mediated ubiquitination of p53 regulates lung adenocarcinoma proliferation.

Biochem Biophys Res Commun 2020 Jun 15. Epub 2020 Jun 15.

Department of Oncology, the People's Hospital of Jianyang, No.180 Hospital Road, Jianyang City, Sichuan Province, China.

Lung adenocarcinoma (LAC) represents approximately 40% of all lung cancer cases and is the leading cause of cancer-associated mortality worldwide. Although combined treatment, including radiotherapy, chemotherapy, surgical treatment and immunotherapy, has been used in treating LAC, the five-year survival rate of patients with LAC has not significantly improved. Therefore, it is vital for cancer research to investigate novel prognostic markers and new targets for molecular targeted therapy in LAC. Read More

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http://dx.doi.org/10.1016/j.bbrc.2020.05.061DOI Listing

Stabilization of C-terminal binding protein 2 by cellular inhibitor of apoptosis protein 1 via BIR domains without E3 ligase activity.

Biochem Biophys Res Commun 2020 Jun 15. Epub 2020 Jun 15.

Department of Biology and Department of Life, Kyung Hee University, Seoul, 02447, Republic of Korea; Nanopharmaceutical Sciences, Kyung Hee University, Seoul, 02447, Republic of Korea. Electronic address:

C-terminal binding protein 2 (CtBP2) is a transcriptional co-repressor that regulates many genes involved in normal cellular events. Because CtBP2 overexpression has been implicated in various human cancers, its protein levels must be precisely regulated. Previously, we reported that CtBP1 and CtBP1-mediated transcriptional repression are regulated by X-linked inhibitor of apoptosis protein (XIAP). Read More

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http://dx.doi.org/10.1016/j.bbrc.2020.05.098DOI Listing

TORC1 regulates G1/S transition and cell proliferation via the E2F homologs MBF and SBF in yeast.

Biochem Biophys Res Commun 2020 Jun 15. Epub 2020 Jun 15.

Graduate School of Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, 422-8021, Japan; Course of Biological Science, Department of Science, Graduate School of Integrated Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, 422-8021, Japan; Department of Bioscience, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, 422-8021, Japan. Electronic address:

The yeast E2F functional homologs MBF (Mbp1/Swi6) and SBF (Swi4/Swi6) complexes are critical transcription factors for G1/S transition. The target of rapamycin complex 1 (TORC1) kinase promotes G1/S transition via upregulation of the G1 cyclin Cln3 that activates MBF and SBF in favorable nutrient conditions. Here, we show evidence that TORC1 directly regulates G1/S transition via MBF and SBF. Read More

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http://dx.doi.org/10.1016/j.bbrc.2020.05.122DOI Listing

Cox2-mediated PGE2 production via p38/JNK-c-fos signaling inhibits cell apoptosis in 3D floating culture clumps of mesenchymal stem cell/extracellular matrix complexes.

Biochem Biophys Res Commun 2020 Jun 15. Epub 2020 Jun 15.

Department of Periodontal Medicine, Graduate School of Biomedical and Health Sciences, Hiroshima University, 1-2-3, Kasumi, Minami-ku, Hiroshima, 734-8553, Japan.

Mesenchymal stem cells (MSCs), a class of adult stem cells, have attracted scientific and medical attention due to their self-renewing properties, multipotency, and trophic factor production. Although MSCs were originally studied on classical two-dimensional (2D) plastic plates, extensive scientific efforts have developed three-dimensional (3D) MSC culture systems, including MSCs spheroids and organoids that can mimic physical conditions. Moreover, we have recently developed 3D culture clumps of MSCs/extracellular matrix (ECM) complexes (C-MSCs) for novel bone regenerative cell therapy. Read More

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http://dx.doi.org/10.1016/j.bbrc.2020.05.100DOI Listing

Synergistic regulation of hepatic Fsp27b expression by HNF4α and CREBH.

Biochem Biophys Res Commun 2020 Jun 15. Epub 2020 Jun 15.

Division of Molecular Science, Graduate School of Science and Technology, Gunma University, Kiryu, Gunma, 376-8515, Japan; Gunma University Center for Food Science and Wellness, Maebashi, Gunma, 371-8510, Japan. Electronic address:

The CIDE (cell death-inducing DFF45-like effector) family composed of CIDEA, CIDEB, CIDEC/FSP27 (fat-specific protein 27), has a critical role in growth of lipid droplets. Of these, CIDEB and CIDEC2/FSP27B are abundant in the liver, and the steatotic livers, respectively. Hepatocyte nuclear factor 4α (HNF4α) has an important role in lipid homeostasis because liver-specific HNF4α-null mice (Hnf4a mice) exhibit hepatosteatosis. Read More

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http://dx.doi.org/10.1016/j.bbrc.2020.05.070DOI Listing

Dimethyl fumarate prevents osteoclastogenesis by decreasing NFATc1 expression, inhibiting of erk and p38 MAPK phosphorylation, and suppressing of HMGB1 release.

Biochem Biophys Res Commun 2020 Jun 15. Epub 2020 Jun 15.

Division of Oral Pharmacology, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki, 852-8588, Japan.

Osteoclasts are multinucleated bone-resorbing cells derived from monocyte/macrophage progenitor cells. Excessive formation and resorbing activities of osteoclasts are involved in the bone-destructive pathologies of rheumatoid arthritis and osteoporosis. Recently, it has been found that nuclear factor erythroid 2-related factor 2 (Nrf2), a transcription factor for anti-oxidative stress genes, functions in osteoclastogenesis. Read More

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http://dx.doi.org/10.1016/j.bbrc.2020.05.088DOI Listing

USP1 inhibitor ML323 enhances osteogenic potential of human dental pulp stem cells.

Biochem Biophys Res Commun 2020 Jun 13. Epub 2020 Jun 13.

Department of Oral and Maxillofacial Surgery, Dental Research Institute, School of Dentistry, Seoul National University, Seoul, 03080, Republic of Korea. Electronic address:

LIM homeobox 8 (LHX8) is expressed during embryonic development of craniofacial tissues, including bone and teeth. In a previous study, the overexpression of LHX8 inhibited osteodifferentiation of human dental pulp stem cells (DPSCs). In this study, a cDNA microarray analysis was performed to reveal the molecular changes which occur in response to LHX8 overexpression in DPSCs and discover possible targets for an osteoinductive agent. Read More

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http://dx.doi.org/10.1016/j.bbrc.2020.05.095DOI Listing

Dcf1 alleviates C99-mediated deficits in drosophila by reducing the cleavage of C99.

Biochem Biophys Res Commun 2020 Jun 12. Epub 2020 Jun 12.

Laboratory of Molecular Neural Biology, School of Life Sciences, Shanghai University, Shanghai, 200444, China. Electronic address:

Alzheimer's disease (AD) is a progressive neurodegenerative disorder. The generation of amyloid-β from the amyloid precursor protein (APP) C-terminal fragment (C99) by γ-secretase cleavage is one of the main pathological mechanisms of AD. Dendritic cell factor 1 (Dcf1) is a membrane protein that was previously found to play a role in the development of AD. Read More

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http://dx.doi.org/10.1016/j.bbrc.2020.05.063DOI Listing

Mutated lamin A modulates stiffness in muscle cells.

Biochem Biophys Res Commun 2020 Jun 12. Epub 2020 Jun 12.

Sorbonne University, INSERM, Institute of Myology, Center of Research in Myology, 75013 Paris, France.

The cytoskeleton is a complex network interlinking filaments that extend throughout the cytoplasm from the nucleus to the plasma membrane. Three major types of filaments are found in the cytoskeleton: actin filaments, microtubules, and intermediate filaments. They play a key role in the ability of cells to both resist mechanical stress and generate force. Read More

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http://dx.doi.org/10.1016/j.bbrc.2020.05.102DOI Listing

NR5A2 synergizes with NCOA3 to induce breast cancer resistance to BET inhibitor by upregulating NRF2 to attenuate ferroptosis.

Biochem Biophys Res Commun 2020 Jun 11. Epub 2020 Jun 11.

MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou, 510631, China; Guangdong Provincial Key Laboratory of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou, 510631, China. Electronic address:

BET inhibitors (BETi) exert an excellent anti-cancer activity in breast cancer. However, the identification of new potential targets to enhance breast cancer sensitivity to BETi is still an enormous challenge. Both NR5A2 and NCOA3 are frequently involved in cancer cells resistance to chemotherapy, also associated with poor prognosis in breast cancer. Read More

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http://dx.doi.org/10.1016/j.bbrc.2020.05.069DOI Listing

Novel insights into biological roles of inducible cAMP early repressor ICER.

Biochem Biophys Res Commun 2020 Jun 11. Epub 2020 Jun 11.

Centre for Functional Genomics and Bio-Chip, Institute of Biochemistry, Faculty of Medicine, University of Ljubljana, Slovenia; Institute of Biochemistry, Faculty of Medicine, University of Ljubljana, Slovenia. Electronic address:

ICER corresponds to a group of alternatively spliced Inducible cAMP Early Repressors with high similarity, but multiple roles, including in circadian rhythm, and are involved in attenuation of cAMP-dependent gene expression. We present experimental and in silico data revealing biological differences between the isoforms with exon gamma (ICER) or without it (ICERγ). Both isoforms are expressed in the liver and the adrenal glands and can derive from differential splicing. Read More

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http://dx.doi.org/10.1016/j.bbrc.2020.05.017DOI Listing

Protein arginine methyltransferase-1 stimulates dopaminergic neuronal cell death in a Parkinson's disease model.

Biochem Biophys Res Commun 2020 Jun 10. Epub 2020 Jun 10.

School of Biological Sciences and Technology, College of Natural Sciences, Chonnam National University, Gwangju, 61186, Republic of Korea; College of Medicine, Chonnam National University, Gwangju, 61469, Republic of Korea. Electronic address:

Recent studies have revealed that protein arginine methyltransferases (PRMTs) are responsible for diverse neurodegenerative diseases. However, their pathophysiological role in dopaminergic neuronal death in Parkinson's disease (PD) has not been evaluated. In this study, we demonstrated that 1-Methyl-4-phenylpyridinium iodide (MPP), rotenone and paraquat, which cause dopaminergic neuronal cell death, increased PRMT1 expression in dopaminergic cell line. Read More

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http://dx.doi.org/10.1016/j.bbrc.2020.05.016DOI Listing

DNA damage/cGAS-triggered up-regulation of MALAT1 promotes undesirable inflammatory responses in radiotherapy of cancer.

Biochem Biophys Res Commun 2020 Aug 9;528(4):746-752. Epub 2020 Jun 9.

Department of Critical Care Medicine & Infection Prevention and Control, The Second People's Hospital of Shenzhen & First Affiliated Hospital of Shenzhen University, Health Science Center, Shenzhen, 518035, PR China. Electronic address:

Radiotherapy is the most common strategy for treating cancer. However, the radiation-induced inflammatory responses, acute or chronic, in the normal tissues of the irradiated region may result in undesirable side effects, such as lung injury and atherosclerosis. MALAT1 is believed to function in the onset, development, progression and metastasis of various cancers. Read More

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http://dx.doi.org/10.1016/j.bbrc.2020.05.064DOI Listing

Modulation of chloroplast components and defense responses during programmed cell death in tobacco infected with Pseudomonas syringae.

Biochem Biophys Res Commun 2020 Aug 9;528(4):753-759. Epub 2020 Jun 9.

School of Life Sciences and Biotechnology, BK21 Plus KNU Creative BioResearch Group, Kyungpook National University, Daegu, 41566, South Korea. Electronic address:

We examined how tobacco plants coordinate chloroplast components and defense responses during Pseudomonas syringae pv. tomato (Pst) infection. Tobacco leaves infiltrated with Pst induced weak necrosis at 24 h post-infiltration (hpi) and severe necrosis at 48 hpi. Read More

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http://dx.doi.org/10.1016/j.bbrc.2020.05.086DOI Listing