84,314 results match your criteria Biochemical and biophysical research communications[Journal]


The role of uric acid in the pathogenesis of diabetic retinopathy based on notch pathway.

Biochem Biophys Res Commun 2018 Jun 19. Epub 2018 Jun 19.

Shanghai General Hospital; Shanghai Jiao Tong University School of Medicine, China. Electronic address:

Objective: Uric acid has been proposed as an independent risk factor of diabetic retinopathy. Although Notch signaling was reported to be affected in the presence of high concentrations of uric acid or glucose, the underlying mechanisms of hyperuricemia through the Notch signaling pathway to promote the development of diabetic retinopathy remain unknown.

Methods: We incubated human retinal endothelial cells (HRECs) with high glucose, high uric acid and high glucose plus high glucose respectively and evaluated the apoptosis rate in different treated cells by Tunel staining. Read More

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mir-127-3p inhibits the proliferation of myocytes by targeting KMT5a.

Biochem Biophys Res Commun 2018 Jun 19. Epub 2018 Jun 19.

State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, China. Electronic address:

MicroRNAs are a class of highly conserved ∼20 nucleotides non-coding RNAs that post-transcriptionally regulate gene expression. Many miRNAs were studied in the development of skeletal muscle, such as miR-1, miR-206, and miR-133. In our previous study, miR-127-3p was found highly expressed in porcine fetal skeletal muscle, whereas the detailed functions of miR-127-3p in muscle development is still unclear. Read More

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June 2018
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PDGF family function and prognostic value in tumor biology.

Biochem Biophys Res Commun 2018 Jun 19. Epub 2018 Jun 19.

Division of Translational Cancer Research, Department of Laboratory Medicine, Lund University, Medicon Village, Scheelevägen 8, 22363, Lund, Sweden. Electronic address:

The development and progression of a tumor depends on the close interaction of malignant cells and the supportive and suppressive tumor microenvironment. Paracrine signaling enables tumor cells to shape the surrounding tissue in order to decrease recognition by the immune system, attract blood vessels to fuel growth, change metabolic programs and induce wound healing programs. In this study, we investigate the role of the platelet-derived growth factor (PDGF) family members PDGFA, PDGFB, PDGFC and PDGFD and their cognate tyrosine kinase receptors PDGFRA and PDGFRB, using publicly available data from The Cancer Genome Atlas and the Human Protein Atlas. Read More

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MiR-455-3p suppresses renal fibrosis through repression of ROCK2 expression in diabetic nephropathy.

Biochem Biophys Res Commun 2018 Jun 19. Epub 2018 Jun 19.

Department of Endocrinology, Shanghai TCM-Integrated Hospital Affiliated Shanghai University of Traditional Chinese Medicine, Baoding Road, Hongkou District, Shanghai, 200082, China.

Emerging evidence has shown that microRNAs (miRNAs) play a mediatory role in the pathogenesis of diabetic nephropathy (DN), but the function of the involved miRNAs is still incomplete. Here, we found that miR-455-3p was down-regulated in the human mesangial cells (HMC) and human proximal tubule epithelial cells (HK-2) stimulated with high glucose (HG) or transforming growth factor beta 1 (TGF-β1). Rho-associated coiled coil-containing protein kinase 2 (ROCK2) was identified as a directed target of miR-455-3p. Read More

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Tissue distribution, subcellular localization, and enzymatic activity analysis of human SIRT5 isoforms.

Biochem Biophys Res Commun 2018 Jun 19. Epub 2018 Jun 19.

National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, 100101, China. Electronic address:

SIRT5 is one of the seven mammalian sirtuins which are NAD-dependent deacylases. In human beings, SIRT5 gene encodes for four SIRT5 protein isoforms, namely SIRT5, SIRT5, SIRT5, and SIRT5. Previous studies have focused mostly on SIRT5. Read More

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Amide-linked local anesthetics preferentially target leukemia stem cell through inhibition of Wnt/β-catenin.

Biochem Biophys Res Commun 2018 Jun 19. Epub 2018 Jun 19.

Department of Anesthesiology, The Second Clinical Medical College, Yangtze University, Jingzhou, Hubei Province, China. Electronic address:

The anti-cancer activities of amide-linked local anesthetics have been demonstrated in various types of bulky/differentiated cancer cells. However, whether these anesthetics also affect biological functions of cancer stem cells is largely unknown. In this study, we systematically investigated the effects of three commonly used amide-linked local anesthetics (ropivacaine, lidocaine and bupivacaine) on leukemia stem cell (LSC) derived from two different leukemia diseases (acute myeloid leukemia, n = 8 and chronic myeloid leukemia, n = 8) as well as normal hematopoietic stem cell (HSC) derived from cord blood donors (n = 8) as comparison. Read More

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The E3 ubiquitin ligase RNF146 promotes colorectal cancer by activating the Wnt/β-catenin pathway via ubiquitination of Axin1.

Biochem Biophys Res Commun 2018 Jun 20. Epub 2018 Jun 20.

Department of Anus Bowel, Xianyang Central Hospital, No. 78 People East Road, Xianyang, Shaanxi, 712000, China. Electronic address:

The E3 ubiquitin ligase ring finger protein 146 (RNF146) has been implicated in tumor development. However, the role and clinical significance of RNF146 in colorectal cancer (CRC) remain unknown. In this study, we reported for the first time that RNF146 was upregulated in CRC tissues as well as in cell lines. Read More

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Unfolding of IgG domains detected by non-reducing SDS-PAGE.

Biochem Biophys Res Commun 2018 Jun 19. Epub 2018 Jun 19.

Department of Pharmacology and Systems Physiology, College of Medicine, University of Cincinnati, 231 Albert Sabin Way, Cincinnati, OH, 45267-0575, USA.

Monoclonal antibodies are very important in modern therapeutics and constitute a substantial percentage of newly approved drugs. Every therapeutic monoclonal antibody must be analyzed for structural and functional integrity, and all protein heterogeneities need to be identified and quantified. The conformational stabilities of the monoclonal antibodies are also important for antibody storage and handling stabilities. Read More

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June 2018
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BMP2/Smad signaling pathway is involved in the inhibition function of fibroblast growth factor 21 on vascular calcification.

Biochem Biophys Res Commun 2018 Jun 19. Epub 2018 Jun 19.

Department of Cardiology, Beijing Anzhen Hospital, Capital Medical University, Beijing Institute of Heart, Lung and Blood Vessel Diseases, Beijing, China. Electronic address:

Vascular calcification is extremely common and associated with major adverse cardiovascular events. Fibroblast growth factor (FGF) 21 has been identified as a potent metabolic regulator and a protector of the cardiovascular system. In this study, we aimed to investigate the effect of FGF21 on calcification of vascular smooth muscle cell (VSMC) and its mechanism. Read More

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June 2018
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The reductase TMX1 contributes to ERAD by preferentially acting on membrane-associated folding-defective polypeptides.

Biochem Biophys Res Commun 2018 Jun 19. Epub 2018 Jun 19.

Università della Svizzera italiana (USI), Faculty of Biomedical Sciences, Institute for Research in Biomedicine, Bellinzona, Switzerland; École Polytéchnique Fédérale de Lausanne (EPFL), School of Life Sciences, CH-1015 Lausanne, Switzerland. Electronic address:

The Endoplasmic Reticulum (ER) is site of production of secretory and membrane proteins in eukaryotic cells. The ER does not contain catabolic devices and misfolded proteins generated in its lumen must be dislocated across the ER membrane before clearance by cytosolic proteasomes (ER-Associated Degradation, ERAD). How misfolded proteins are dislocated across the ER membrane is a matter of controversy. Read More

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June 2018
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Regulation of the regulator of G protein signaling 2 expression and cellular localization by PKA and PKC pathways in mouse granulosa cells.

Biochem Biophys Res Commun 2018 Jun 19. Epub 2018 Jun 19.

Department of Physiology, School of Medicine, National Yang-Ming University, Taipei, Taiwan. Electronic address:

G protein-coupled receptor (GPCR) activation-mediated PKA and PKC pathways have been recognized to be important in ovarian physiology. Expression of regulator of G-protein signaling 2 (RGS2) has been reported in ovarian granulosa cells. The detailed mechanisms in PKA- and PKC-regulated RGS2 expression and cellular translocation in granulosa cells remain mostly unclear. Read More

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Further characterization of the Maillard deglycase DJ-1 and its prokaryotic homologs, deglycase 1/Hsp31, deglycase 2/YhbO, and deglycase 3/YajL.

Biochem Biophys Res Commun 2018 Jun 19. Epub 2018 Jun 19.

UMR 8601 CNRS, Laboratoire de Chimie et Biochimie Pharmacologiques et Toxicologiques, Université Paris Descartes-Sorbonne Paris Cité, 75270, Paris, France.

We reported recently that the Parkinsonism-associated protein DJ-1 and its bacterial homologs Hsp31, YhbO and YajL function as deglycases that repair proteins and nucleotides from endogeneous glycation by glyoxal and methylglyoxal, two reactive by-products of glucose metabolism responsible for up to 60% of glycation damage. Here, we show that DJ-1, deglycase 1 and deglycase 2 repair glyoxal- and methylglyoxal-glycated substrates, whereas deglycase 3 principally repairs glyoxal-glycated substrates. Moreover, deglycase 1 and 2 are overexpressed in stationary phase, whereas deglycase 3 is steadily expressed throughout bacterial growth. Read More

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June 2018
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A natural compound, aristoyagonine, is identified as a potent bromodomain inhibitor by mid-throughput screening.

Biochem Biophys Res Commun 2018 Jun 19. Epub 2018 Jun 19.

Bio & Drug Discovery Division, Korea Research Institute of Chemical Technology, Daejeon, 34114, Republic of Korea; Department of Medicinal Chemistry and Pharmacology, Korea University of Science and Technology, Daejeon, 34113, Republic of Korea. Electronic address:

Bromodomain-containing protein 4 (Brd4) is known to play a key role in tumorigenesis. It binds acetylated histones to regulate the expression of numerous genes. Because of the importance of brd4 in tumorigenesis, much research has been undertaken to develop brd4 inhibitors with therapeutic potential. Read More

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June 2018
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Lactic acid induces lactate transport and glycolysis/OXPHOS interconversion in glioblastoma.

Biochem Biophys Res Commun 2018 Jun 18. Epub 2018 Jun 18.

Department of Biochemistry and Molecular Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, 610041, China.

The Warburg effect is a dominant phenotype of most tumor cells. Recent reports have shown that the Warburg effect can be reprogrammed by the tumor microenvironment. Lactic acidosis and glucose deprivation are the common adverse microenvironments in solid tumor. Read More

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Suppression of cleavage factor Im 25 promotes the proliferation of lung cancer cells through alternative polyadenylation.

Biochem Biophys Res Commun 2018 Jun 18. Epub 2018 Jun 18.

Nanjing Medical University, Nanjing, Jiangsu, China.

Background: Non-small cell lung cancer (NSCLC) is a life-threatening disease that has a poor prognosis and low survival rate. Cleavage factor Im 25 (CFIm25) is a RNA-binding protein that if down-regulated causes 3'UTR shortening and thus promotes the transcript stability of target genes. It is not clear whether CFIm25 and alternative polyadenylation (APA) play a role during cancer development. Read More

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CircRNA circ_0026344 as a prognostic biomarker suppresses colorectal cancer progression via microRNA-21 and microRNA-31.

Biochem Biophys Res Commun 2018 Jun 18. Epub 2018 Jun 18.

Department of Medical Oncology, Xuzhou Central Hospital, XuZhou, 221009, China. Electronic address:

Recently, circular RNA (circRNA) is identified as a novel class of noncoding RNA with important roles in human diseases, such as cancer. However, how circRNA participates in colorectal cancer (CRC) remains unclear. In this study, we aimed to illustrate the role of circ_0026344 in CRC progression. Read More

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Mouse model of chorea-acanthocytosis exhibits male infertility caused by impaired sperm motility as a result of ultrastructural morphological abnormalities in the mitochondrial sheath in the sperm midpiece.

Biochem Biophys Res Commun 2018 Jun 18. Epub 2018 Jun 18.

Department of Psychiatry, Kagoshima University Graduate School of Medical and Dental Sciences, 8-35-1 Sakuragaoka, Kagoshima, 890-8520, Japan.

Chorea-acanthocytosis (ChAc) is an autosomal recessive hereditary disease characterized by neurodegeneration in the striatum and acanthocytosis caused by loss-of-function mutations in the Vacuolar Protein Sorting 13 Homolog A (VPS13A) gene, which encodes chorein. We previously produced a ChAc-model mouse with a homozygous deletion of exons 60-61 in Vps13a, which corresponded to the human disease mutation. We found that male ChAc-model mice exhibited complete infertility as a result of severely diminished sperm motility. Read More

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UBE2S enhances the ubiquitination of p53 and exerts oncogenic activities in hepatocellular carcinoma.

Biochem Biophys Res Commun 2018 Jun 19. Epub 2018 Jun 19.

Department of Pathology, The Affiliated Hexian Memorial Hospital of Southern Medical University, Guangzhou, China. Electronic address:

Ubiquitin-conjugating enzyme E2S (UBE2S) plays pivotal roles in the progression of human cancers. However, its clinical significance and role in hepatocellular carcinoma (HCC) remain unknown. Here, we show that UBE2S is upregulated in HCC and exhibits oncogenic activities via enhancing the ubiquitination of p53. Read More

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Mitoflash lights single mitochondrial dynamics events in mature cardiomyocytes.

Biochem Biophys Res Commun 2018 Jun 18. Epub 2018 Jun 18.

Institute for Regenerative Medicine, Shanghai East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai, 200092, China. Electronic address:

Mitochondria, the powerhouse of eukaryotic cells, are highly dynamic organelle. Mitochondrial fission, fusion, kissing and contraction have been reported over and over again in non-static cells, such as fibroblast, with tubular mitochondrial networks. Even though the fluorescence propagation among mitochondria of mature cardiomyocytes had been captured using mitochondrial matrix targeted photoactivatable GFP (PAGFP) or MitoDendra proteins, there are no direct evidence that single real time mitochondrial dynamics events exist in mature cardiomyocytes with ball-like mitochondria. Read More

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Escherichia coli tolerance of ultraviolet radiation by in vivo expression of a short peptide designed from late embryogenesis abundant protein.

Biochem Biophys Res Commun 2018 Jun 18. Epub 2018 Jun 18.

Department of Biological Functions Engineering, Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology, Kitakyushu Science and Research Park, Kitakyushu, Fukuoka, Japan. Electronic address:

Ultraviolet (UV) radiation causes damage in all living organisms, including DNA damage that leads to cell death. Herein, we provide a new technique for UV radiation protection through intracellular short peptide expression. The late embryogenesis abundant (LEA) peptide, which functions as a shield that protects macromolecules from various abiotic stress, was obtained from the Polypedilum vanderplanki group 3 LEA protein. Read More

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PTP1B promotes the malignancy of ovarian cancer cells in a JNK-dependent mechanism.

Biochem Biophys Res Commun 2018 Jun 18. Epub 2018 Jun 18.

Department of Obstetrics & Gynecology, Shanxi Academy of Medical Sciences & Shanxi Da Yi Hospital, Taiyuan, Shanxi, 030032, PR China.

Ovarian cancer is the leading cause of death from gynecological malignancies in women. Diagnosis at the early stage remains challenging and efficient treatment is still highly needed. The development and progression of this cancer is associated with many genetic and epigenetic changes, representing the dysregulation of a highly complex signaling network. Read More

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Circular RNA HIPK3 promotes gallbladder cancer cell growth by sponging microRNA-124.

Biochem Biophys Res Commun 2018 Jun 18. Epub 2018 Jun 18.

Research Institute of General Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing, China. Electronic address:

Recent studies have implied that circHIPK3, an abundant circular RNA (circRNA), participates in tumorigenesis and cancer progression. Its expression and potential functions in human gallbladder cancer were examined in this study. We show that circHIPK3 is upregulated in human gallbladder cancer cells. Read More

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TRPV4 promotes the migration and invasion of glioma cells via AKT/Rac1 signaling.

Biochem Biophys Res Commun 2018 Jun 19. Epub 2018 Jun 19.

Department of Neurosurgery, Daping Hospital, The Army Medical University, Chongqing, 400042, China. Electronic address:

Experimental evidence indicates a critical role of TRPV4 (Transient Receptor Potential Vanilloid 4) in controlling the cell migratory activity of multiple tumors. However, the oncogenic role of TRPV4 in glioma still remains elusive. In this study, we tried to investigate the oncogenic role of TRPV4 in glioma. Read More

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June 2018
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17β-Estradiol protects serum deprivation-induced apoptosis and expression of MMP-3 and MMP-13 through promoting autophagy in rat nucleus pulposus cells.

Biochem Biophys Res Commun 2018 Jun 18. Epub 2018 Jun 18.

Department of Orthopedics, The Second Affiliated Hospital of Nanchang University, Nanchang, 330006, JiangXi Province, China. Electronic address:

Serum deprivation is a likely contributor to intervertebral disc (IVD) degeneration (IVDD).17β-estradiol (E2) have been noted to protect nucleus pulposus cells (NPCs) against apoptosis. Autophagy and apoptosis play a paramount role in maintaining the homeostasis of IVD. Read More

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Long non-coding RNA MALAT1 interacts with transcription factor Foxo1 to regulate SIRT1 transcription in high glucose-induced HK-2 cells injury.

Biochem Biophys Res Commun 2018 Jun 18. Epub 2018 Jun 18.

Department of Nephrology, The First Affiliated Hospital of Soochow University, Suzhou, 215006, China.

Background: Tubular injury is considered as a crucial pathological feature of diabetic nephropathy. LncRNA MALAT1 is involved in diabetic complications. Hence the role of MALAT1 in high glucose-induced renal tubular epithelial cells (HK-2) injury deserves investigation. Read More

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MCPIP1 negatively regulate cellular antiviral innate immune responses through DUB and disruption of TRAF3-TBK1-IKKε complex.

Biochem Biophys Res Commun 2018 Jun 16. Epub 2018 Jun 16.

Division of Infection and Immunity, Department of Biological Technology, Beijing Institute of Radiation Medicine, Beijing, 100850, China. Electronic address:

IFNβ innate immune plays an essential role in antiviral immune. Previous reports suggested that many important regulatory proteins in innate immune pathway may be modified by ubiquitin and that many de-ubiquitination (DUB) proteins may affect immunity. Monocyte chemotactic protein-inducing protein 1 (MCPIP1), one of the CCCH Zn finger-containing proteins, was reported to have DUB function, but its effect on IFNβ innate immune was not fully understood. Read More

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Comparative folding analyses of unknotted versus trefoil-knotted ornithine transcarbamylases suggest stabilizing effects of protein knots.

Biochem Biophys Res Commun 2018 Jun 16. Epub 2018 Jun 16.

Institute of Biological Chemistry, Academia Sinica, Taipei, 11529, Taiwan; Institute of Biochemical Sciences, National Taiwan University, Taipei, 10617, Taiwan. Electronic address:

Ornithine transcarbamylases (OTCs) are conserved enzymes involved in arginine biosynthesis in microbes and the urea cycle in mammals. Recent bioinformatics analyses identified two unique OTC variants, N-succinyl-l-ornithine transcarbamylase from Bacteroides fragilis (BfSOTC) and N-acetyl-l-ornithine transcarbamylase from Xanthomonas campestris (XcAOTC). These two variants diverged from other OTCs during evolution despite sharing the common tertiary and quaternary structures, with the exception that the substrate recognition motifs are topologically knotted. Read More

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June 2018
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Acid ceramidase inhibition sensitizes human colon cancer cells to oxaliplatin through downregulation of transglutaminase 2 and β1 integrin/FAK-mediated signalling.

Biochem Biophys Res Commun 2018 Jun 16. Epub 2018 Jun 16.

University of Rijeka Department of Biotechnology, Centre for High-Throughput Technologies, Radmile Matejčić 2, 51000 Rijeka, Croatia. Electronic address:

Acid ceramidase (ASAH1) has been implicated in the progression and chemoresistance in different cancers. Its role in colon cancer biology and response to standard chemotherapy has been poorly addressed so far. Here, we have investigated ASAH1 expression at the protein level in human colon cancer cell lines and tissues from colon cancer patients, and have examined in vitro the possible link between ASAH1 expression and functional activity of p53 protein whose inactivation is associated with the progression from adenoma to malignant tumour in colon cancer. Read More

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June 2018
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Scallop-derived plasmalogens attenuate the activation of PKCδ associated with the brain inflammation.

Biochem Biophys Res Commun 2018 Jun 16. Epub 2018 Jun 16.

Department of Medicine and Biosystemic Science Kyushu University Faculty of Medicine, Graduate School of Medical Sciences, Kyushu University, Fukuoka, 812-8582, Japan.

Activation of protein kinase C delta (PKCδ) has been linked to the neuroinflammation but the relationship with the various neurodegenerative diseases including the Alzheimer's disease (AD) was mostly elusive. In the AD brains, the special phospholipids, ethanolamine plasmalogens (Pls), were found to be reduced and our previous study showed that these lipids possess neuroprotective and anti-inflammatory functions. In the present study, we could find that these lipids can significantly attenuate the microglial expression of PKCδ in the neuroinflammation model and in the AD model mice brains. Read More

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Plasmodium falciparum histidine triad protein and calmodulin modulates calcium homeostasis and intracellular proteolysis.

Biochem Biophys Res Commun 2018 Jun 20. Epub 2018 Jun 20.

Departamento de Biociências, Universidade Federal de São Paulo, Santos, Brazil. Electronic address:

Calcium signaling has an essential role in fundamental processes of Plasmodium life cycle, including migration, cell invasion and parasite development. Two important players in calcium homeostasis, the Histidine Triad (HIT) protein that is implicated in calcium signaling in mammalian cells and calmodulin, which is a classic calcium sensor in eukaryotes are present in Plasmodium falciparum, however theirs function is unknown in the parasite. Here, we investigated the involvement of the P. Read More

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SIS3, a specific inhibitor of smad3, attenuates bleomycin-induced pulmonary fibrosis in mice.

Biochem Biophys Res Commun 2018 Jun 15. Epub 2018 Jun 15.

Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, China; Department of Immunology, Tongji University School of Medicine, Shanghai, China. Electronic address:

Pulmonary fibrosis (PF) is a fatal respiratory disease with no effective medical treatments available. TGF-β/Smads signaling has been implicated to play an essential in the pathogenesis of PF, in which Smad3 act as the integrator of pro-fibrosis signals. In this study, we determined the effect of SIS3, a specific inhibitor of Smad3, in an experimental mouse model of lung fibrosis. Read More

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Synthesis and detoxification of nitric oxide in the plant beneficial rhizobacterium Bacillus amyloliquefaciens SQR9 and its effect on biofilm formation.

Biochem Biophys Res Commun 2018 Jun 16. Epub 2018 Jun 16.

Jiangsu Provincial Key Lab for Organic Solid Waste Utilization, National Engineering Research Center for Organic-based Fertilizers, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, Nanjing Agricultural University, Nanjing, 210095, PR China; Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, 100081, PR China. Electronic address:

Nitric oxide (NO) is an important gas signal that regulates many biological processes, and due to the high nitrogen recycling activity in the rhizosphere, NO is an important signaling molecule in this region. Thus, an understanding of the effect of NO on the rhizomicrobiome, especially on plant beneficial rhizobacteria, is important for the use of these bacteria in agriculture. In this study, the effect of exogenous NO on the beneficial rhizobacterium Bacillus amyloliquefaciens SQR9 was investigated. Read More

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Disruption of IDH2 attenuates lipopolysaccharide-induced inflammation and lung injury in an α-ketoglutarate-dependent manner.

Biochem Biophys Res Commun 2018 Jun 16. Epub 2018 Jun 16.

School of Life Sciences and Biotechnology, BK21 Plus KNU Creative BioResearch Group, College of Natural Sciences, Kyungpook National University, Taegu, Republic of Korea. Electronic address:

Acute lung injury (ALI) is an acute failure of the respiratory system with unacceptably high mortality, for which effective treatment is urgently necessary. Infiltrations by immune cells, such as leukocytes and macrophages, are responsible for the inflammatory response in ALI, which is characterized by excessive production of pro-inflammatory mediators in lung tissues exposed to various pathogen-associated molecules such as lipopolysaccharide (LPS) from microbial organisms. α-Ketoglutarate (α-KG) is a key metabolic intermediate and acts as a pro-inflammatory metabolite, which is responsible for LPS-induced proinflammatory cytokine production through NF-κB signaling pathway. Read More

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June 2018
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Long noncoding RNA ANCR suppresses bone formation of periodontal ligament stem cells via sponging miRNA-758.

Biochem Biophys Res Commun 2018 Jun 15. Epub 2018 Jun 15.

Department of Oral and Maxillofacial Surgery, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, PR China; Guangdong Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, PR China. Electronic address:

Long noncoding RNAs (lncRNAs) were proposed to be important regulators influencing various differentiation processes. Yet, the molecular mechanisms of lncRNAs governing osteogenic differentiation of Periodontal Ligament Stem Cells (PDLSCs) remain unclear. Here, PDLSCs were isolated from normal periodontal ligament of human (PDL) whereas P-PDLSCs were isolated from periodontitis affected PDL. Read More

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Notch signaling regulates the expression of glycolysis-related genes in a context-dependent manner during embryonic development.

Biochem Biophys Res Commun 2018 Jun 15. Epub 2018 Jun 15.

Graduate School of Pharmaceutical Sciences, Chiba University, Chiba 260-8675, Japan. Electronic address:

Glycolysis, the classic pathway for producing energy, has been known to be involved in neural development. Notch signaling also contributes to neural development and regulation of glycolysis in various tissues. However, the role of Notch signaling in glycolysis-related gene regulation during neural development is poorly understood. Read More

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June 2018
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Fibromodulin modulates myoblast differentiation by controlling calcium channel.

Biochem Biophys Res Commun 2018 Jun 16. Epub 2018 Jun 16.

Department of Medical Biotechnology, Yeungnam University, Gyeongsan, 38541, Republic of Korea. Electronic address:

Fibromodulin (FMOD) is a proteoglycan present in extracellular matrix (ECM). Based on our previous findings that FMOD controls myoblast differentiation by regulating the gene expressions of collagen type I alpha 1 (COL1α1) and integral membrane protein 2 A (Itm2a), we undertook this study to investigate relationships between FMOD and calcium channels and to understand further the mechanism by which they control myoblast differentiation. Gene expression studies and luciferase reporter assays showed FMOD affected calcium channel gene expressions by regulating calcium channel gene promoter, and patch-clamp experiments showed both L- and T-type calcium channel currents were almost undetectable in FMOD knocked down cells. Read More

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Paraquat toxicity is attenuated by 4-phenylbutyrate-induced phosphorylation of ERK2 via PI3K in A549 cells.

Biochem Biophys Res Commun 2018 Jun 18. Epub 2018 Jun 18.

Department of Legal Medicine, Asahikawa Medical University, Asahikawa, Hokkaido, 070-8510, Japan.

Paraquat (PQ) is a widely used herbicide in the world despite being highly toxic to humans. PQ causes fatal damage to multiple organs, especially the lungs. While oxidative stress is the main toxic mechanism of PQ, there is no established standard therapy for PQ poisoning. Read More

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June 2018
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Lactate enhanced the effect of parathyroid hormone on osteoblast differentiation via GPR81-PKC-Akt signaling.

Biochem Biophys Res Commun 2018 Jun 15. Epub 2018 Jun 15.

Department of Occupational Health, Wuxi Center for Disease Control and Prevention, Wuxi, Jiangsu, China. Electronic address:

Osteoblast uses aerobic glycolysis to meet the metabolic needs in differentiation process. Lactate, the end product of glycolysis, presents in the environment with elevated PTH and osteoblast differentiation. Although previous findings showed that lactate promoted osteoblast differentiation, whether lactate affects PTH-mediated osteoblast differentiation is unclear. Read More

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Plasma exosomes regulate systemic blood pressure in rats.

Biochem Biophys Res Commun 2018 Jun 15. Epub 2018 Jun 15.

Laboratory of Veterinary Pharmacology, School of Veterinary Medicine, Kitasato University, Towada, Aomori, 034-0828, Japan. Electronic address:

Exosomes, the smallest extracellular vesicle, contain various molecules and mediate cell-cell communication. A number of studies demonstrate exosomes are involved in important physiological and pathological processes. Moreover, microRNA (miRNA) regulating hypertension development through the suppression of certain translation was recently reported. Read More

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Structural analysis of substrate recognition by glucose isomerase in Mn binding mode at M2 site in S. rubiginosus.

Biochem Biophys Res Commun 2018 Jun 16. Epub 2018 Jun 16.

Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, Republic of Korea; Institute of Life Science and Natural Resources, Korea University, Seoul, Republic of Korea. Electronic address:

Glucose isomerase (GI) catalyzes the reversible enzymatic isomerization of d-glucose and d-xylose to d-fructose and d-xylulose, respectively. This is one of the most important enzymes in the production of high-fructose corn syrup (HFCS) and biofuel. We recently determined the crystal structure of GI from S. Read More

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Gene expression profile of TLR7 signaling pathway in the liver of rhesus rotavirus-induced murine biliary atresia.

Biochem Biophys Res Commun 2018 Jun 14. Epub 2018 Jun 14.

Department of Pediatric Surgery, Shengjing Hospital, China Medical University, Shenyang, 110004, PR China. Electronic address:

Purpose: To identify genes potentially involved in the pathogenesis of bile duct obstruction in biliary atresia (BA).

Methods: We used rhesus rotavirus (RRV) Balb/c mouse BA model to study BA. Liver and serum samples were harvested from BA and normal control (NC) groups at 1, 3, 5, 7, 10 and 14 days postinoculation. Read More

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Introduction of a glycosylation site in the constant region decreases the aggregation of adalimumab Fab.

Biochem Biophys Res Commun 2018 Jun 18. Epub 2018 Jun 18.

Faculty of Pharmaceutical Sciences, Sojo University, Japan. Electronic address:

The production of therapeutic monoclonal antibodies is costly; therefore, antigen-binding fragments (Fabs) can be used instead. However, their tendency toward aggregation can reduce the half-life in the plasma and the therapeutic effectiveness. To examine the effect of glycosylation on the properties of the Fab of a therapeutic antibody, an N-glycosylation site was introduced at position 178 of the H-chain constant region of adalimumab Fab through site-directed mutagenesis of L178 N (H:L178 N Fab), and then H:L178 N Fab was expressed in Pichia pastoris. Read More

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Knockout of CNR1 prevents metabolic stress-induced cardiac injury through improving insulin resistance (IR) injury and endoplasmic reticulum (ER) stress by promoting AMPK-alpha activation.

Biochem Biophys Res Commun 2018 Jun 14. Epub 2018 Jun 14.

Department of Pharmacy, The First Affiliated Hospital of Xinxiang Medical College, Weihui, 453100, China. Electronic address:

Obesity and diabetes are associated with diabetic cardiomyopathy (DCM). However, the pathogenesis of DCM is not fully understood. Cannabinoid receptor gene (CNR1) has been a drug target for the treatment of obesity. Read More

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Pulsed electromagnetic field induces Ca-dependent osteoblastogenesis in C3H10T1/2 mesenchymal cells through the Wnt-Ca/Wnt-β-catenin signaling pathway.

Biochem Biophys Res Commun 2018 Jun 16. Epub 2018 Jun 16.

Department of Orthopaedics, Zhujiang Hospital, Southern Medical University, Guanzhou 510280, Guangdong, PR China. Electronic address:

Pulsed electromagnetic fields (PEMFs) are effective in healing fractures and improving osteoporosis. However, their effect on mesenchymal cells remains largely unknown. In this study, the effects of PEMF on osteoblastogenesis and its underlying molecular signaling mechanisms were systematically investigated in C3H10T1/2 cells. Read More

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Early emergence of de novo EGFR T790 M gatekeeper mutations during erlotinib treatment in PC9 non-small cell lung cancer cells.

Biochem Biophys Res Commun 2018 Jun 14. Epub 2018 Jun 14.

Department of Nanobiomedical Science, Dankook University, Cheonan, 31116, Republic of Korea. Electronic address:

The emergence of the T790 M gatekeeper mutation in the Epidermal Growth Factor Receptor (EGFR) gene is an important mechanism that can lead to the acquired resistance to EGFR-targeted tyrosine kinase inhibitors such as erlotinib or gefitinib. These drugs have been used in treating a subset of non-small cell lung cancer (NSCLC) patients harboring EGFR activating mutations. Here we investigated the paths leading to the acquisition of the T790 M mutation by establishing an erlotinib resistant PC9 cell model harboring ectopically introduced EGFR cDNA. Read More

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Shelterin differentially respond to oxidative stress induced by TiO-NPs and regulate telomere length in human hepatocytes and hepatocarcinoma cells in vitro.

Biochem Biophys Res Commun 2018 Jun 15. Epub 2018 Jun 15.

School of Life Sciences, Yunnan Normal University, Kunming, Yunnan, 650500, China; Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Ministry of Education, Kunming, Yunnan, 650500, China. Electronic address:

Titanium dioxide nanoparticles (TiO-NPs) have raised serious attention for their widely use and potential adverse effects on human mainly due to producing ROS. However, the influence of TiO-NPs on telomere maintaining has not been studied clearly. Shelterin plays core roles in telomere length (TL) regulation. Read More

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Design and development of PEGylated liposomal formulation of HER2 blocker Lapatinib for enhanced anticancer activity and diminshed cardiotoxicity.

Biochem Biophys Res Commun 2018 Jun 13. Epub 2018 Jun 13.

Division of Toxicology and Experimental Medicine, CSIR-Central Drug Research Institute, Lucknow, Uttar Pradesh, 226031, India; Academy of Scientific and Innovative Research, (AcSIR), New Delhi, 110025, India. Electronic address:

Breast cancer is most frequently diagnosed cancer and fifth leading cause of death in women. About 20-30% of all breast cancers overexpress HER2/neu receptors. Lapatinib is a dual tyrosin kinase inhibitor of EGFR and HER2. Read More

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Characterization of molecular interactions between Zika virus protease and peptides derived from the C-terminus of NS2B.

Biochem Biophys Res Commun 2018 Jun 13. Epub 2018 Jun 13.

Experimental Therapeutics Centre, Agency for Science, Technology and Research (A*STAR), 31 Biopolis Way, Nanos, #03-01, 138669, Singapore. Electronic address:

Zika virus (ZIKV) protease is a two-component complex in which NS3 contains the catalytic triad and NS2B cofactor region is important for protease folding and activity. A protease construct-eZiPro lacking the transmembrane domains of NS2B was designed. Structural study on eZiPro reveals that the Thr-Gly-Lys-Arg (TGKR) sequence at the C-terminus of NS2B binds to the active site after cleavage. Read More

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MIF protects against oxygen-glucose deprivation-induced ototoxicity in HEI-OC1 cochlear cells by enhancement of Akt-Nrf2-HO-1 pathway.

Biochem Biophys Res Commun 2018 Jun 16. Epub 2018 Jun 16.

Department of Otolaryngology Head and Neck Surgery, The Affiliated Huaian No.1 People's Hospital of Nanjing Medical University, Huaian, 223300, Jinagsu Province, China.

Ischemia and oxidative stress play crucial roles in the pathophysiology of sudden sensorineural hearing loss (SSNHL). Macrophage migration inhibitory factor (MIF) is a pro-inflammatory cytokine and serves an important role in hearing function. The present study was designed to evaluate the effect of MIF on oxygen-glucose deprivation (OGD)-induced ototoxicity and to elucidate its molecular mechanism. Read More

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A site-specific branching poly-glutamate tag mediates intracellular protein delivery by cationic lipids.

Biochem Biophys Res Commun 2018 Jun 15. Epub 2018 Jun 15.

Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Department of Chemistry, Tsinghua University, Beijing 100084, PR China. Electronic address:

Intracellular protein delivery is of significance for cellular protein analysis and therapeutic development, but remains challenging technically. Herein, we report a general and highly potent strategy for intracellular protein delivery based on commercially available cationic lipids. In this strategy, a designed double branching poly-glutamate tag is site-specifically attached onto the C-terminal of protein cargos via expressed protein ligation (EPL), which mediates the entrapment of proteins into cationic liposomes driven by electrostatic interaction. Read More

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June 2018
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