412 results match your criteria Biochemia Medica[Journal]


A paradigmatic case of haemolysis and pseudohyperkalemia in blood gas analysis.

Biochem Med (Zagreb) 2019 Feb;29(1):011003

Section of Clinical Biochemistry, University of Verona, Verona, Italy.

A 51-year old male patient was admitted to the hospital with acute dyspnea and history of chronic asthma. Venous blood was drawn into a 3.0 mL heparinized syringe and delivered to the laboratory for blood gas analysis (GEM Premier 4000, Instrumentation Laboratory), which revealed high potassium value (5. Read More

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http://dx.doi.org/10.11613/BM.2019.011003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6366946PMC
February 2019
4 Reads

Plasma free DNA: Evaluation of temperature-associated storage effects observed for Roche Cell-Free DNA collection tubes.

Biochem Med (Zagreb) 2019 Feb;29(1):010904

Institute of Clinical Chemistry and Laboratory Medicine, General Hospital Steyr, Steyr, Austria.

Introduction: Standardized pre-analytical blood sample procedures for the analysis of circulating cell-free DNA (ccfDNA) are still not available. Therefore, the present study aimed at evaluating the impact of storage conditions related to different times (24 and 48 h) and temperatures (room temperature (RT) and 4 - 8 °C) on the plasma ccfDNA concentration of blood samples drawn into Cell-Free DNA collection tubes (Roche Diagnostics GmbH, Mannheim, Germany).

Materials And Methods: Venous blood from 30 healthy individuals was collected into five 8. Read More

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http://dx.doi.org/10.11613/BM.2019.010904DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6366949PMC
February 2019

Improving home haemodialysis: Stability evaluation of routine clinical chemistry analytes in blood samples of haemodialysis patients.

Biochem Med (Zagreb) 2019 Feb;29(1):010709

Department of Nephrology and Hypertension, University Medical Center Utrecht, Utrecht, The Netherlands.

Introduction: A growing number of dialysis patients is treated with home haemodialysis. Our current pre-analytical protocols require patients to centrifuge the blood sample and transfer the plasma into a new tube at home. This procedure is prone to errors and precludes accurate bicarbonate measurement, required for determining dialysate bicarbonate concentration and maintaining acid-base status. Read More

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http://dx.doi.org/10.11613/BM.2019.010709DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6366947PMC
February 2019
1 Read

Analytical verification of 12 most commonly used urine dipsticks in Croatia: comparability, repeatability and accuracy.

Biochem Med (Zagreb) 2019 Feb;29(1):010708

Department of Medical Laboratory Diagnostics, University Hospital "Sveti Duh", Zagreb, Croatia.

Introduction: Variability among manufacturers of urine dipsticks, respective to their accuracy and measurement range, may lead to diagnostic errors and thus create a serious risk for the patient. Our aims were to determine the level of agreement between 12 most commonly used urine dipsticks in Croatia, examine their accuracy for glucose and total protein and to test their repeatability.

Materials And Methods: A total of 75 urine samples were used to examine comparability and accuracy of 12 dipstick brands (Combur 10 TestM, ChoiceLine 10, Combur 10 TestUX, ComboStik 10M, ComboStik 11M, CombiScreen 11SYS, CombiScreen 10SL, Combina 13, Combina 11S, Combina 10M, UriGnost 11, Multistix 10SG). Read More

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http://dx.doi.org/10.11613/BM.2019.010708DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6366948PMC
February 2019
5 Reads
2.400 Impact Factor

Faecal calprotectin determination: impact of preanalytical sample treatment and stool consistency on within- and between-method variability.

Biochem Med (Zagreb) 2019 Feb;29(1):010707

Department of Laboratory Diagnostics, General Hospital Pula, Pula, Croatia.

Introduction: We assessed the differences in faecal calprotectin (FC) concentrations measured by two assays depending on the stool consistency and extraction method.

Materials And Methods: Stool samples were extracted using the EliA Stool Extraction Kit, Calex® Cap extraction device and respective weighing methods, while FC concentrations were measured using the EliA Calprotectin and Bühlmann fCAL® Turbo method and checked for within- and between-method variability with regard to extraction method and stool consistency category. Extraction yield was evaluated for impact of different sample incubation time (10 min and 1 h) in extraction buffer for both methods and for impact of different initial sample dilutions (1:50, 1:100, 1:500) for fCAL® Turbo method. Read More

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http://dx.doi.org/10.11613/BM.2019.010707DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6366951PMC
February 2019

Changes of salivary biomarkers under different storage conditions: effects of temperature and length of storage.

Biochem Med (Zagreb) 2019 Feb;29(1):010706

Department of Animal and Food Science, Faculty of Veterinary Medicine, Autonomous University of Barcelona, Barcelona, Spain.

Introduction: In this report, we aimed to examine the stability of various analytes in saliva under different storage conditions.

Materials And Methods: Alpha-amylase (AMY), cholinesterase (CHE), lipase (Lip), total esterase (TEA), creatine kinase (CK), aspartate aminotransferase (AST), lactate dehydrogenase (LD), lactate (Lact), adenosine deaminase (ADA), Trolox equivalent antioxidant capacity (TEAC), ferric reducing ability (FRAS), cupric reducing antioxidant capacity (CUPRAC), uric acid (UA), catalase (CAT), advanced oxidation protein products (AOPP) and hydrogen peroxide (HO) were colorimetrically measured in saliva obtained by passive drool from 12 healthy voluntary donors at baseline and after 3, 6, 24, 72 hours, 7 and 14 days at room temperature (RT) and 4 ºC, and after 14 days, 1, 3 and 6 months at - 20 ºC and - 80 ºC.

Results: At RT, changes appeared at 6 hours for TEA and HO; 24 hours for Lip, CK, ADA and CUPRAC; and 72 hours for LD, Lact, FRAS, UA and AOPP. Read More

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http://dx.doi.org/10.11613/BM.2019.010706DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6366950PMC
February 2019

Higher serum lectin-like oxidized low-density lipoprotein receptor-1 in patients with stable coronary artery disease is associated with major adverse cardiovascular events: A multicentre pilot study.

Biochem Med (Zagreb) 2019 Feb;29(1):010705

Department of Cardiology, Union Hospital, Fujian Medical University, Fuzhou, P. R. China.

Introduction: Lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) is involved in the pathophysiology of atherosclerosis and acute coronary syndromes (ACS). Circulating soluble LOX-1 (sLOX-1) has been linked to the risk of coronary artery disease (CAD). Our aim was to test if baseline serum sLOX-1 was associated with major adverse cardiovascular events (MACE) in patients with stable CAD. Read More

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http://dx.doi.org/10.11613/BM.2019.010705DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6366952PMC
February 2019
1 Read

Case report: An index of suspicion in hyponatraemia.

Biochem Med (Zagreb) 2019 Feb 15;29(1):011002. Epub 2018 Dec 15.

Division of Chemical Pathology, National Health Laboratory Service (NHLS) and University of Stellenbosch, Tygerberg Hospital, Cape Town, South Africa.

Serum indices can give valuable information and should be interpreted as a result. Lipaemia can influence results through different mechanisms, an important one being the electrolyte exclusion effect. A case of pseudohyponatraemia due to this is reported. Read More

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http://dx.doi.org/10.11613/BM.2019.011002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6294159PMC
February 2019
5 Reads

Falsely prolonged activated partial thromboplastin time - a pre- and post-analytical issue.

Biochem Med (Zagreb) 2019 Feb 15;29(1):011001. Epub 2018 Dec 15.

Department of Clinical Biochemistry and Pharmacology, Odense University Hospital, Odense, Denmark.

This case highlights two common pre-analytical problems identified in routine coagulation testing of activated partial thromboplastin time (aPTT), which were overlooked because of a concurrent flag code indicating no coagulation and the result was replaced by asterisks. It concerns a boy with gastrointestinal bleeding and prolonged aPTT > 300 seconds, which raised the suspicion of haemophilia. When all other coagulation parameters (including specific coagulation factors VIII and IX) turned out to be normal, aPTT was re-measured using another analysis principle, which revealed a normal aPTT. Read More

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http://dx.doi.org/10.11613/BM.2019.011001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6294156PMC
February 2019

Mistaken assumptions drive new Six Sigma model off the road.

Biochem Med (Zagreb) 2019 Feb 15;29(1):010903. Epub 2018 Dec 15.

Westgard QC, Madison, USA.

Oosterhuis and Coskun recently proposed a new model for applying the Six Sigma concept to laboratory measurement processes. In criticizing the conventional Six Sigma model, the authors misinterpret the industrial basis for Six Sigma and mixup the Six Sigma "counting methodology" with the "variation methodology", thus many later attributions, conclusions, and recommendations are also mistaken. Although the authors attempt to justify the new model based on industrial principles, they ignore the fundamental relationship between Six Sigma and the process capability indices. Read More

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http://dx.doi.org/10.11613/BM.2019.010903DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6294151PMC
February 2019
5 Reads

Sigma metric revisited: True known mistakes.

Biochem Med (Zagreb) 2019 Feb 15;29(1):010902. Epub 2018 Dec 15.

Department of Medical Biochemistry, Acıbadem Mehmet Ali Aydınlar University, School of Medicine, Istanbul, Turkey.

Six Sigma methodology has been used successfully in industry since the mid-1980s. Unfortunately, the same success has not been achieved in laboratory medicine. In this case, although the multidisciplinary structure of laboratory medicine is an important factor, the concept and statistical principles of Six Sigma have not been transferred correctly from industry to laboratory medicine. Read More

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http://www.biochemia-medica.com/en/journal/29/1/10.11613/BM.
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http://dx.doi.org/10.11613/BM.2019.010902DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6294160PMC
February 2019
5 Reads

Effects of different anticoagulants on glycated albumin quantification.

Biochem Med (Zagreb) 2019 Feb 15;29(1):010901. Epub 2018 Dec 15.

Clinical Laboratory, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico, Milano, Italy.

Introduction: In the last 20 years glycated albumin (GA) measurement has been demonstrated to be a reliable glycation marker and recently as the most innovative one in western countries. Glycated albumin has been already adopted by some Asian countries due to its usefulness in diabetes screening. The aim of the present study was to investigate for the first time the effects of different anticoagulants on GA assay. Read More

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http://www.biochemia-medica.com/en/journal/29/1/10.11613/BM.
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http://dx.doi.org/10.11613/BM.2019.010901DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6294155PMC
February 2019
13 Reads

Analysis of laboratory critical values at a referral Spanish tertiary university hospital.

Biochem Med (Zagreb) 2019 Feb 15;29(1):010704. Epub 2018 Dec 15.

Laboratori Clínic, Hospital Universitari de Bellvitge, Hospitalet de Llobregat, Barcelona, Spain.

Introduction: The aim of this study was to analyse critical value data from our laboratory and compare our critical value reporting policy with others in the literature.

Materials And Methods: Analysis of critical values was performed on data obtained over a 6-month period in a tertiary university hospital.

Results: We identified 5723 critical values, of which approximately 80% came from STAT testing (4577), 15% from routine inpatients testing (884) and 5% from routine outpatients testing (262). Read More

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http://dx.doi.org/10.11613/BM.2019.010704DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6294153PMC
February 2019

Fast skeletal troponin I, but not the slow isoform, is increased in patients under statin therapy: a pilot study.

Biochem Med (Zagreb) 2019 Feb 15;29(1):010703. Epub 2018 Dec 15.

Section of Medical Biochemistry, Molecular Biology and Genetics, Department of Biomedical and Specialist Surgical Sciences, University of Ferrara, Ferrara, Italy.

Introduction: Statin therapy is often associated with muscle complaints and increased serum creatine kinase (CK). However, although essential in determining muscle damage, this marker is not specific for skeletal muscle. Recent studies on animal models have shown that slow and fast isoforms of skeletal troponin I (ssTnI and fsTnI, respectively) can be useful markers of skeletal muscle injury. Read More

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http://dx.doi.org/10.11613/BM.2019.010703DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6294157PMC
February 2019

Prognostic value of procalcitonin and lipopolysaccharide binding protein in cancer patients with chemotherapy-associated febrile neutropenia presenting to an emergency department.

Biochem Med (Zagreb) 2019 Feb 15;29(1):010702. Epub 2018 Dec 15.

Biochemistry Department, University Hospital Santa Lucía, Cartagena, Spain.

Introduction: Cancer patients with chemotherapy-induced febrile neutropenia are a heterogeneous group with a significant risk of serious medical complications. In these patients, the Multinational Association for Supportive Care in Cancer (MASCC) score is the most widely used tool for risk-stratification. The aim of this prospective study was to analyse the value of procalcitonin (PCT) and lipopolysaccharide binding protein (LBP) to predict serious complications and bacteraemia in cancer patients with febrile neutropenia, compared with MASCC score. Read More

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http://dx.doi.org/10.11613/BM.2019.010702DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6294152PMC
February 2019
2 Reads

Standardization in laboratory medicine: Two years' experience from category 1 EQA programs in Spain.

Biochem Med (Zagreb) 2019 Feb 15;29(1):010701. Epub 2018 Dec 15.

Spanish Society of Laboratory Medicine (SEQCML), Analytical Quality Commission, Barcelona, Spain.

Introduction: Standardization is the ability to obtain interchangeable results leading to same medical interpretation. External quality assessment (EQA) is the main support of the on-going harmonization initiatives. Aim of study was to evaluate results obtained from two years category 1 EQA program experience in Spain and determine the impact of applying this type of EQA program on the analytical standardization. Read More

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http://dx.doi.org/10.11613/BM.2019.010701DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6294154PMC
February 2019
18 Reads

A review of urinary angiotensin converting enzyme 2 in diabetes and diabetic nephropathy.

Biochem Med (Zagreb) 2019 Feb 15;29(1):010501. Epub 2018 Dec 15.

Department of laboratory medicine, Shengjing Hospital of China Medical University, Shenyang, People's Republic of China.

Urinary angiotensin converting enzyme 2 (ACE2) is significantly increased in diabetes and diabetic nephropathy. While studies on its clinical significance are still underway, its urinary expression, association with metabolic and renal parameters has been in the recent past considerably studied. The recent studies have demystified urine ACE2 in many ways and suggested the roles it could play in the management of diabetic nephropathy. Read More

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http://www.biochemia-medica.com/en/journal/29/1/10.11613/BM.
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http://dx.doi.org/10.11613/BM.2019.010501DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6294158PMC
February 2019
11 Reads

Correcting duplicate publications: follow up study of MEDLINE tagged duplications.

Biochem Med (Zagreb) 2019 Feb 15;29(1):010201. Epub 2018 Dec 15.

Department of Research in Biomedicine and Health, University of Split School of Medicine, Split, Croatia.

Introduction: As MEDLINE indexers tag similar articles as duplicates even when journals have not addressed the duplication(s), we sought to determine the reasons behind the tagged duplications, and if the journals had undertaken or had planned to undertake any actions to address them.

Materials And Methods: On 16 January 2013, we extracted all tagged duplicate publications (DPs), analysed published notices, and then contacted MEDLINE and editors regarding cases unaddressed by notices. For non-respondents, we compared full text of the articles. Read More

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http://dx.doi.org/10.11613/BM.2019.010201DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6294161PMC
February 2019

Confidence interval for quantiles and percentiles.

Biochem Med (Zagreb) 2019 Feb 15;29(1):010101. Epub 2018 Dec 15.

Department of Human Physiology and Pharmacology, University of Rome Sapienza, Rome, Italy.

Quantiles and percentiles represent useful statistical tools for describing the distribution of results and deriving reference intervals and performance specification in laboratory medicine. They are commonly intended as the sample estimate of a population parameter and therefore they need to be presented with a confidence interval (CI). In this work we discuss three methods to estimate CI on quantiles and percentiles using parametric, nonparametric and resampling (bootstrap) approaches. Read More

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http://dx.doi.org/10.11613/BM.2019.010101DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6294150PMC
February 2019

Effect of cold agglutinins on red blood cell parameters in a trauma patient: a case report.

Biochem Med (Zagreb) 2018 Oct;28(3):031001

Department of clinical chemistry, Sestre milosrdnice University Hospital Center, Zagreb, Croatia.

The presence of cold agglutinins (CAs) in samples intended for complete blood count (CBC) using automated haematology analysers might cause serious preanalytical errors. In this report we describe the case of a 90-year old female patient admitted to the Emergency department following trauma injuries. A blood testing on admission revealed surprisingly low red blood cell count (0. Read More

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http://dx.doi.org/10.11613/BM.2018.031001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6214689PMC
October 2018
18 Reads

Biotin and high-sensitivity cardiac troponin T assay.

Biochem Med (Zagreb) 2018 Oct;28(3):030901

General Biochemistry Department, Rouen University Hospital, Rouen, France.

Introduction: The high-sensitivity cardiac troponin T assay of Roche Diagnostics is known to have interference with high concentrations of biotin as this assay uses biotin-streptavidin binding as detection method. As studies so far have not shown if different biotin concentrations could have diverse influence on various troponin concentrations and whether interference could be removed by available protocol within corresponding turnaround time we aimed to investigate it.

Materials And Methods: Plasma samples were spiked with different concentration of biotin solution. Read More

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http://dx.doi.org/10.11613/BM.2018.030901DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6214697PMC
October 2018
21 Reads

Metabolic follow-up of a Croatian patient with gyrate atrophy and a new mutation in the gene: a case report.

Biochem Med (Zagreb) 2018 Oct;28(3):030801

Department of Pediatrics, University Hospital Center Zagreb, Zagreb, Croatia.

Gyrate atrophy (GA) of the choroid and retina is a rare autosomal recessive disorder that occurs due to deficiency of the mitochondrial enzyme ornithine aminotransferase (OAT). Hyperornithinemia causes degeneration of the retina with symptoms like myopia, reduced night vision and progressive vision loss. Our patient is a 10-year-old girl with impaired vision and strabismus. Read More

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http://dx.doi.org/10.11613/BM.2018.030801DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6214690PMC
October 2018
10 Reads

The diagnostic accuracy of clinical and laboratory parameters in the diagnosis of acute appendicitis in the adult emergency department population - a case control pilot study.

Biochem Med (Zagreb) 2018 Oct;28(3):030712

Department of Medical Laboratory Diagnostics, University Hospital "Sveti Duh", Zagreb, Croatia.

Introduction: The evaluation of patients with suspected appendicitis strives to identify all patients with presenting symptoms while minimizing negative appendectomy rate. The aim of the study was to identify the optimal combination of clinical and laboratory parameters that should facilitate the emergency department surgeon's definite decision.

Materials And Methods: The study group comprised 120 patients with suspicion of acute appendicitis (AA). Read More

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http://dx.doi.org/10.11613/BM.2018.030712DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6214693PMC
October 2018

A collaborative study by the Working Group on Hemostasis and Thrombosis of the Italian Society of Clinical Biochemistry and Clinical Molecular Biology (SIBioC) on the interference of haemolysis on five routine blood coagulation tests by evaluation of 269 paired haemolysed/non-haemolysed samples.

Biochem Med (Zagreb) 2018 Oct;28(3):030711

Section of Clinical Biochemistry, University of Verona, Verona, Italy.

Introduction: Haemolysis is the leading cause of sample rejection in laboratory haemostasis. Most studies focused on artificially haemolysed samples. The aim of this study was a prospective assessment of spontaneous haemolysis on haemostasis tests, by comparing results of haemolysed (H) new, non-haemolysed (NH) specimens, collected within 4hrs. Read More

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http://dx.doi.org/10.11613/BM.2018.030711DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6214687PMC
October 2018
6 Reads

Calcium-stimulated calcitonin - The "new standard" in the diagnosis of thyroid C-cell disease - clinically relevant gender-specific cut-off levels for an "old test".

Biochem Med (Zagreb) 2018 Oct;28(3):030710

Division of General Surgery, Department of Surgery, Medical University of Vienna, Vienna, Austria.

Introduction: Pentagastrin (Pg) stimulated calcitonin (sCT) was used to enhance accuracy in medullary thyroid cancer (MTC) diagnosis. As it is now unavailable, calcium (Ca) has been recommended as an alternative. The aim of this study was to define gender-specific cut-off values to predict MTC in patients with elevated basal CT (bCT) following Pg-sCT and Ca-sCT stimulation and to compare the time courses of CT release during stimulation. Read More

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http://dx.doi.org/10.11613/BM.2018.030710DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6214694PMC
October 2018
11 Reads

Reducing test request for anti-thyroglobulin and anti-thyroid peroxidase antibodies: trends before and after interventions based on rejection rules and profile management.

Biochem Med (Zagreb) 2018 Oct;28(3):030709

Department of Clinical Medicine, MiBioPath group, Universidad Católica San Antonio de Murcia (UCAM), Murcia, Spain.

Introduction: The objective of this study was to identify trends in requests for anti-thyroid peroxidase antibodies (TPOab) and anti-thyroglobulin antibodies (TGab) tests before and after applying set of interventions based on rejection rules and profile management.

Materials And Methods: Trend analysis was made at semester time intervals (from May-October 2010 to May-October 2017), before and after the intervention semester (May-October 2016). Number of tests (N) TPOab and TGab / 1000 total requests based on total N of both tests and total N of biochemical analysis laboratory requests, was calculated. Read More

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http://dx.doi.org/10.11613/BM.2018.030709DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6214703PMC
October 2018

Evaluation of the analytical performances of the Cobas c513 analyser for HbA assay.

Biochem Med (Zagreb) 2018 Oct;28(3):030708

Biochemistry Department, University Hospital of Reims, Reims, France.

Introduction: Haemoglobin A (HbA) is considered to be the gold standard for the follow-up of glycaemic control in patients with diabetes mellitus and is also a diagnostic tool. Accordingly, reliable and efficient methods must be used for its quantification. Roche Diagnostics have recently adapted the Tina-quant® HbA Third Generation immunoassay on a fully dedicated analyser, the Cobas c513, which allows a high throughput of up to 400 samples hour. Read More

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http://www.biochemia-medica.com/en/journal/28/3/10.11613/BM.
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http://dx.doi.org/10.11613/BM.2018.030708DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6214704PMC
October 2018
25 Reads

Analysis of selected T cell subsets in peripheral blood after exhaustive effort among elite soccer players.

Biochem Med (Zagreb) 2018 Oct;28(3):030707

Department of Biological Bases of Physical Education, Faculty of Physical Education and Health Promotion, University of Szczecin, Szczecin, Poland.

Introduction: Physical exercise induces developing of naïve T lymphocyte subsets into polarised effector ones by immune system. The aim of the study was to examine the influence of exhaustive effort on the selected Th cell subsets and inflammation markers among soccer players.

Materials And Methods: Fourteen soccer players aged 18 (16-21) years old performed the progressive efficiency test on mechanical treadmill. Read More

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http://www.biochemia-medica.com/en/journal/28/3/10.11613/BM.
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http://dx.doi.org/10.11613/BM.2018.030707DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6214701PMC
October 2018
8 Reads

Computer physician order entry (CPOE) as a strategy to estimate laboratory activity and costs associated with cancer clinical trials.

Biochem Med (Zagreb) 2018 Oct;28(3):030706

Laboratory of Biochemistry, Valencia University Clinic Hospital, Valencia, Spain.

Introduction: Most of clinical laboratories are not properly reimbursed for their activity related to clinical trials (CTs) conducted in their institutions due to a lack of measurement strategies. We implemented a specific computer physician order entry (CPOE) environment for CTs in order to facilitate ordering to providers and estimate the associated costs to be compared with the standard of care (SOC).

Materials And Methods: Four specific electronic formularies, restricted to two new virtual CTs clinical services (onco - CT and haemo - CT), were implemented in January 2015. Read More

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http://www.biochemia-medica.com/en/journal/28/3/10.11613/BM.
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http://dx.doi.org/10.11613/BM.2018.030706DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6214692PMC
October 2018
13 Reads

Effect of sample processing and time delay on cell count and chemistry tests in cerebrospinal fluid collected from drainage systems.

Biochem Med (Zagreb) 2018 Oct;28(3):030705

Faculty of Pharmacy and Biochemistry, University of Zagreb, Zagreb, Croatia.

Introduction: Cerebrospinal fluid (CSF) from extra-ventricular drainage (EVD) systems is routinely analysed to diagnose EVD-related bacterial meningitis. We investigated the effect of time delay and sample processing on cell count and basic biochemistry results in EVD CSF to define optimal turnaround time and whether manual and automated cell counting are comparable in such samples.

Materials And Methods: In total, 32 EVD CSF samples were analysed. Read More

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http://dx.doi.org/10.11613/BM.2018.030705DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6214696PMC
October 2018
3 Reads

Haematology specimen acceptability: a national survey in Chinese laboratories.

Biochem Med (Zagreb) 2018 Oct;28(3):030704

National Center for Clinical Laboratories, Beijing Hospital, National Center of Gerontology, Beijing, China.

Introduction: Specimen adequacy is a crucial preanalytical factor affecting accuracy and usefulness of test result. The aim of this study was to determine the frequency and reasons for rejected haematology specimens, preanalytical variables which may affect specimen quality, and consequences of rejection, and provide suggestions on monitoring quality indicators as to obtain a quality improvement.

Materials And Methods: A cross-sectional survey was conducted and a questionnaire was sent to 1586 laboratories. Read More

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http://dx.doi.org/10.11613/BM.2018.030704DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6214705PMC
October 2018
1 Read

Detection of annexin A8 antibodies in serum of patients with antiphospholipid syndrome.

Biochem Med (Zagreb) 2018 Oct;28(3):030703

Institute for Clinical Chemistry, Medical Faculty, University of Cologne, Cologne, Germany.

Introduction: Antibodies specific for annexin A8 (AnxA8) have not been investigated in patients suffering from antiphospholipid syndrome (APS) yet. The aim of this study was to compare the presence of AnxA8 antibodies in serum of APS patients with that of age-matched healthy controls and to investigate whether AnxA8 antibodies are potential biomarkers for APS.

Materials And Methods: We enrolled 22 APS patients and 22 healthy controls in this case-control study. Read More

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http://dx.doi.org/10.11613/BM.2018.030703DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6214702PMC
October 2018
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Significance of LDL and HDL subclasses characterization in the assessment of risk for colorectal cancer development.

Biochem Med (Zagreb) 2018 Oct;28(3):030703

Department of Medical Biochemistry, Faculty of Pharmacy, University of Belgrade, Belgrade, Serbia.

Introduction: Dyslipidaemia contributes to the occurrence of colorectal cancer (CRC). We hypothesized that qualitative changes of lipoproteins are associated with the risk for CRC development. This study analyses low-density lipoprotein (LDL) and high-density lipoprotein (HDL) diameters, as well as distribution of LDL and HDL subclasses in patients with CRC, with an aim to determine whether advanced lipid testing might be useful in predicting the risk for the onset of this malignancy. Read More

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http://dx.doi.org/10.11613/BM.2018.030713DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6214700PMC
October 2018
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Association of abdominal aortic aneurysm diameter with insulin resistance index.

Biochem Med (Zagreb) 2018 Oct;28(3):030702

Université Côte d'Azur, CHU, Inserm, C3M, Nice, France.

Introduction: Epidemiological studies have highlighted a negative association between diabetes and abdominal aortic aneurysm (AAA). The aim of this study was to investigate the association between insulin resistance and AAA size.

Materials And Methods: This prospective cross sectional monocentric study analysed fasting blood samples from 55 patients with AAA eligible for surgical repair. Read More

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http://dx.doi.org/10.11613/BM.2018.030702DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6214695PMC
October 2018
9 Reads

Improvement of neutrophil gelatinase-associated lipocalin sensitivity and specificity by two plasma measurements in predicting acute kidney injury after cardiac surgery.

Biochem Med (Zagreb) 2018 Oct;28(3):030701

Unit of Laboratory Medicine, Centro Cardiologico ''Monzino'', IRCCS, Milan, Italy.

Introduction: Acute kidney injury (AKI) remains among the most severe complication after cardiac surgery. The aim of this study was to evaluate the neutrophil gelatinase-associated lipocalin (NGAL) as possible biomarker for the prediction of AKI in an adult cardiac population.

Materials And Methods: Sixty-nine consecutive patients who underwent cardiac surgeries in our hospital were prospectively evaluated. Read More

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http://dx.doi.org/10.11613/BM.2018.030701DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6214698PMC
October 2018
17 Reads

Red blood cell distribution width in pregnancy: a systematic review.

Biochem Med (Zagreb) 2018 Oct;28(3):030502

Department of Biomedical Sciences, University of Sassari, Sassari, Italy.

Anisocytosis has been associated with the severity and prognosis of several acute and chronic diseases, as well as physiological conditions such as pregnancy. Anisocytosis is quantified by the red blood cell distribution width (RDW), expressed as the ratio, multiplied by 100, between the standard deviation (SD) of red blood cell volumes and the mean corpuscular volume, or as the SD of erythrocyte volumes (RDW-SD). The aim of the present review was to report the state of the art on the physiological values and the putative diagnostic and prognostic roles of RDW in complicated pregnancy. Read More

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http://dx.doi.org/10.11613/BM.2018.030502DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6214699PMC
October 2018
21 Reads
2.400 Impact Factor

"Ultra-sensitive" cardiac troponins: Requirements for effective implementation in clinical practice.

Biochem Med (Zagreb) 2018 Oct;28(3):030501

Leon H. Charney Division of Cardiology, New York University School of Medicine, New York, USA.

The measurement of cardiac troponins, either cardiac troponin I or T, has become the culprit of clinical decision making in patients with suspected acute coronary syndrome (ACS), especially in those with non-ST elevation myocardial infarction (NSTEMI). The leading analytical mainstays of cardiac troponin immunoassays include the limit of blank (LoB), limit of detection (LoD), functional sensitivity, the 99 percentile of a healthy reference population, along with the percentage of "ostensibly healthy" subjects displaying measurable values < 99 percentile. The latest generation of cardiac troponin immunoassays, conventionally defined as "high-sensitive" (HS), is characterized by a LoD over 100-fold lower compared to the first commercialized techniques and a percentage of measurable values consistently > 50% in the general healthy population. Read More

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http://dx.doi.org/10.11613/BM.2018.030501DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6214691PMC
October 2018
2 Reads

Informed consent and ethics committee approval in laboratory medicine.

Biochem Med (Zagreb) 2018 Oct;28(3):030201

Research Integrity Editor, Biochemia Medica.

Informed consent is a process in which a human subject who is to participate in research needs to give his or her consent after being properly informed of the expected benefits as well as the potential harm of the research that will be performed. The function and purpose of the research ethics committee is to ensure that the research that will take place is in accordance with the relevant ethical standards. This means that the committee must assess the appropriateness of the design of the study reviewed. Read More

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http://dx.doi.org/10.11613/BM.2018.030201DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6214688PMC
October 2018
15 Reads

Evaluating analytical quality in clinical biochemistry laboratory using Six Sigma.

Biochem Med (Zagreb) 2018 Jun;28(2):020904

Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong, People's Republic of China.

Introduction: In recent years, Six Sigma metrics has became the hotspot in all trades and professions, which contributes a general procedure to explain the performance on sigma scale. Nowadays, many large companies, such as General Healthcare, Siemens, ., have applied Six Sigma to clinical medicine and achieved satisfactory results. Read More

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http://dx.doi.org/10.11613/BM.2018.020904DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6039163PMC
June 2018
3 Reads

Using Sigma metrics to establish analytical product performance requirements and optimize analytical performance of an diagnostic assay using a theoretical total PSA assay as an example.

Biochem Med (Zagreb) 2018 Jun;28(2):020903

Abbott, Abbott Park, IL, USA.

Introduction: Establishing analytical performance requirements for in vitro diagnostic (IVD) assays is a challenging process. Manufacturers try to optimize analytical performance by choosing amongst many combinations of different product performance characteristics. Sigma metrics and method decision charts can be helpful aids in choosing appropriate analytical performance requirements. Read More

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http://dx.doi.org/10.11613/BM.2018.020903DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6039165PMC

Utilization of a healthcare failure mode and effects analysis to identify error sources in the preanalytical phase in two tertiary hospital laboratories.

Biochem Med (Zagreb) 2018 Jun;28(2):020713

University Espiritu Santo, Guayaquil, Ecuador.

Introduction: The presence of errors in the preanalytical phase is a thoroughly studied problem. A strategy to increase their source detection might be the use of the Healthcare Failure Mode and Effects Analysis (HFMEA). The aim of this study is improving the capacity of identifying sources of error during the preanalytical period in samples provided by primary care clinics (PCC) with the use of the HFMEA as a tool in the laboratories of two tertiary hospitals. Read More

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http://dx.doi.org/10.11613/BM.2018.020713DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6039167PMC
June 2018
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Evaluation of the analytical performances of Cobas 6500 and Sysmex UN series automated urinalysis systems with manual microscopic particle counting.

Biochem Med (Zagreb) 2018 Jun;28(2):020712

Department of Medical Biochemistry, School of Medicine, Ataturk University, Erzurum, Turkey.

Introduction: Automated urinalysis systems are valuable tools in clinical laboratories, especially those with a high work load. The objective of this study was to compare the analytical performance of Sysmex UN series urine analyser, which may become a new one in our laboratory, with the Cobas 6500 automated urine analyser, which is used in our laboratory for a long time. For comparisons, manual microscopical examination was accepted as reference method. Read More

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http://dx.doi.org/10.11613/BM.2018.020712DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6039169PMC
June 2018
16 Reads

Comparison between Sigma metrics in four accredited Egyptian medical laboratories in some biochemical tests: an initiative towards sigma calculation harmonization.

Biochem Med (Zagreb) 2018 Jun;28(2):020711

Private laboratory, Helwan University, Alexandria, Egypt.

Introduction: Analytical quality is an essential requirement for best practice in any medical laboratory. Lack of a harmonized approach for sigma calculation is considered an obstacle in the objective comparability of analytical performance among laboratories adopting sigma metrics. It is urgently needed that all laboratory professionals interested in the analytical quality to work hard towards harmonization protocol for sigma calculation in order to properly select their analytical goals. Read More

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http://dx.doi.org/10.11613/BM.2018.020711DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6039160PMC
June 2018
2 Reads

Comprehensive evaluation of the internal and external quality control to redefine analytical quality goals.

Biochem Med (Zagreb) 2018 Jun;28(2):020710

Laboratorio de Análisis Clínicos, Quality Assurance & Quality Control Department, Montevideo, Uruguay.

Introduction: The aim of this work is to design a selection algorithm for total allowable error (TEa) source using a graphic tool that, by integrating internal (IQC) and external (EQC) quality control performances, enables the laboratory to evaluate which TEa source better fits the test analytical performance.

Materials And Methods: Two analytical performance indicators (bias and Sigma metrics) were estimated for 23 biochemistry tests during 2016. Bias was estimated on the EQC, and Sigma metrics was calculated through the results obtained in the IQC. Read More

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http://dx.doi.org/10.11613/BM.2018.020710DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6039162PMC
June 2018
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Application of Sigma metrics in assessing the clinical performance of verified non-verified reagents for routine biochemical analytes.

Biochem Med (Zagreb) 2018 Jun;28(2):020709

Department of Medical Laboratory, Shengjing Hospital of China Medical University, Shenyang, China.

Introduction: Sigma metrics analysis is considered an objective method to evaluate the performance of a new measurement system. This study was designed to assess the analytical performance of verified non-verified reagents for routine biochemical analytes in terms of Sigma metrics.

Materials And Methods: The coefficient of variation (CV) was calculated according to the mean and standard deviation (SD) derived from the internal quality control for 20 consecutive days. Read More

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http://dx.doi.org/10.11613/BM.2018.020709DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6039166PMC
June 2018
2 Reads

Sigma metrics for assessing the analytical quality of clinical chemistry assays: a comparison of two approaches: Electronic supplementary material available online for this article.

Biochem Med (Zagreb) 2018 Jun;28(2):020708

Department of Laboratory Medicine, Peking Union Medical College Hospital, Chinese Academic Medical Science and Peking Union Medical College, Beijing, P.R. China.

Introduction: Two approaches were compared for the calculation of coefficient of variation (CV) and bias, and their effect on sigma calculation, when different allowable total error (TEa) values were used to determine the optimal method for Six Sigma quality management in the clinical laboratory.

Materials And Methods: Sigma metrics for routine clinical chemistry tests using three systems (Beckman AU5800, Roche C8000, Siemens Dimension) were determined in June 2017 in the laboratory of Peking Union Medical College Hospital. Imprecision (CV%) and bias (bias%) were calculated for ten routine clinical chemistry tests using a proficiency testing (PT)- or an internal quality control (IQC)-based approach. Read More

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http://dx.doi.org/10.11613/BM.2018.020708DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6039159PMC
June 2018
28 Reads
2.400 Impact Factor

Risk analysis and assessment based on Sigma metrics and intended use.

Biochem Med (Zagreb) 2018 Jun;28(2):020707

Department of Clinical Medical Laboratory, Peking University Shenzhen Hospital, Futian District, Shenzhen, China.

Introduction: In order to ensure the quality in clinical laboratories and meet the low risk requirements of patients and clinicians, a risk analysis and assessment model based on Sigma metrics and intended use was constructed, based on which differential sigma performance () expectations of 42 analytes were developed.

Materials And Methods: Failure mode and effects analysis was applied to produce an analytic risk rating based on three factors, each test of which was graded as follows: 1) Sigma metrics; 2) the severity of harm; 3) intended use. By multiplying the score of Sigma metrics by the score of severity of harm by the score of intended use, each was assigned a typical risk priority number (RPN), with RPN ≤ 25 rated as low risk. Read More

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http://dx.doi.org/10.11613/BM.2018.020707DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6039164PMC
June 2018
1 Read
2.400 Impact Factor

Corrigendum to: The potential of component-resolved diagnosis in laboratory diagnostics of allergy.

Biochem Med (Zagreb) 2018 06;28(2):020702

Department of medical biochemistry and hematology, Faculty of Pharmacy and Biochemistry, University of Zagreb.

[This corrects the article DOI: 10.11613/BM.2018. Read More

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http://dx.doi.org/10.11613/BM.2018.021201DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6039168PMC

Sigma metrics in laboratory medicine revisited: We are on the right road with the wrong map.

Biochem Med (Zagreb) 2018 Jun;28(2):020503

Department of Medical Biochemistry, Acibadem University School of Medicine, Istanbul, Turkey.

Reliable procedures are needed to quantify the performance of instruments and methods in order to increase the quality in clinical laboratories. The Sigma metrics serves that purpose, and in the present study, the current methods for the calculation of the Sigma metrics are critically evaluated. Although the conventional model based on permissible (or allowable) total error is widely used, it has been shown to be flawed. Read More

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http://dx.doi.org/10.11613/BM.2018.020503DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6039171PMC
June 2018
25 Reads

Analytical Sigma metrics: A review of Six Sigma implementation tools for medical laboratories.

Biochem Med (Zagreb) 2018 Jun;28(2):020502

Westgard QC, Madison, USA.

Sigma metrics have become a useful tool for all parts of the quality control (QC) design process. Through the allowable total error model of laboratory testing, analytical assay performance can be judged on the Six Sigma scale. This not only allows benchmarking the performance of methods and instruments on a universal scale, it allows laboratories to easily visualize performance, optimize the QC rules and numbers of control measurements they implement, and now even schedule the frequency of running those controls. Read More

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http://dx.doi.org/10.11613/BM.2018.020502DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6039161PMC
June 2018
2 Reads