488 results match your criteria Biochemia Medica[Journal]


A case of severe pseudohyperkalaemia due to muscle contraction.

Biochem Med (Zagreb) 2020 Jun;30(2):021004

Clinical Department of Laboratory Medicine, University Hospitals Leuven, Leuven, Belgium.

Introduction: Severe hyperkalaemia is a serious medical condition requiring immediate medical attention. Before medical treatment is started, pseudohyperkalaemia has to be ruled out.

Case Description: A 10-month old infant presented to the emergency department with fever and coughing since 1 week. Read More

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http://dx.doi.org/10.11613/BM.2020.021004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7271752PMC

Case report of a phantom pheochromocytoma.

Biochem Med (Zagreb) 2020 Jun;30(2):021003

Department of Endocrinology, Cork University Hospital, Ireland.

Plasma free metanephrines or urinary fractionated metanephrines are the biochemical tests of choice for the diagnosis of pheochromocytoma as they have greater sensitivity and specificity than catecholamines for pheochromocytoma detection. This case highlights the preanalytical factors which can influence metanephrine measurement and cause a false positive result. It describes a patient with a high pre-test probability of pheochromocytoma due to hypertension and a past medical history of adrenalectomy for a purported pheochromocytoma in her home country. Read More

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http://dx.doi.org/10.11613/BM.2020.021003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7271750PMC

Verification of the Canadian Laboratory Initiative on Paediatric Reference Intervals (CALIPER) reference values in Croatian children and adolescents.

Biochem Med (Zagreb) 2020 Jun;30(2):020710

Department of Laboratory Diagnostics, Children's Hospital Zagreb, Zagreb, Croatia.

Introduction: The aim of this study was to examine whether the Canadian Laboratory Initiative on Paediatric Reference Intervals (CALIPER) could be applied to Croatian children and adolescents.

Materials And Methods: A total of 295 outpatient healthy children and adolescents of age 1 to 18 were selected using the direct sampling method. According to current guidelines, 20 samples were tested for each of a total of 51 reference intervals for ferritin, cortisol, dehydroepiandrosterone sulfate, follicle stimulating hormone, lutein stimulating hormone, prolactin, progesterone, sex hormone binding globulin, thyroid stimulating hormone, total testosterone, total thyroxine and total triiodothyronine. Read More

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http://dx.doi.org/10.11613/BM.2020.020710DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7271758PMC

Patient-based quality control for glucometers: using the moving sum of positive patient results and moving average.

Biochem Med (Zagreb) 2020 Jun;30(2):020709

Department of Laboratory Medicine, National University Hospital, Singapore.

Introduction: The capability of glucometer internal quality control (QC) in detecting varying magnitude of systematic error (bias), and the potential use of moving sum of positive results (MovSum) and moving average (MA) techniques as potential alternatives were evaluated.

Materials And Methods: The probability of error detection using routine QC and manufacturer's control limits were investigated using historical data. Moving sum of positive results and MA algorithms were developed and optimized before being evaluated through numerical simulation for false positive rate and probability of error detection. Read More

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http://dx.doi.org/10.11613/BM.2020.020709DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7271757PMC

Quality improvement project: Reducing non-conformities of the samples for haemostasis testing in a secondary healthcare centre through the nurses' education in phlebotomy.

Biochem Med (Zagreb) 2020 Jun;30(2):020708

Department of transfusion medicine, General hospital Pula, Pula, Croatia.

Introduction: Poor compliance to the current guidelines and lack of knowledge among nurses about proper blood sampling is set as the study hypothesis. Here is presented a quality improvement project with following aims: a) to identify the most prevalent non-conformity of the samples for haemostasis testing, b) to identify the cause of sample non-conformity, c) to perform corrective action(s) and d) to assess the effectiveness of the corrective action(s).

Materials And Methods: The rate of non-conformity of samples collected for haemostasis tests was established for hospital wards with inpatients. Read More

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http://dx.doi.org/10.11613/BM.2020.020708DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7271753PMC

The results of external quality assessment programme on urine leukocyte and erythrocyte counting in Poland.

Biochem Med (Zagreb) 2020 Jun;30(2):020707

Department of Clinical Chemistry, Medical University of Gdańsk, Gdańsk, Poland.

Introduction: Urine particle analysis is an important diagnostic tool. The aim of this study was to evaluate the quality of urine leukocyte (WBC) and erythrocyte (RBC) counting results obtained with manual and automated methods in Polish laboratories participating in the international external quality assessment (EQA) programme.

Materials And Methods: 1400 WBC and RBC counting results were obtained from 183 laboratories in EQA surveys organised by Labquality (Helsinki, Finland) from 2017 to 2019. Read More

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http://dx.doi.org/10.11613/BM.2020.020707DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7271756PMC

Survey on request form content and result reporting in therapeutic drug monitoring service among laboratories in Czechia and Slovakia.

Biochem Med (Zagreb) 2020 Jun;30(2):020706

Institute of Environmental and Chemical Engineering, University of Pardubice, Czech Republic.

Introduction: The aim of the study was to investigate current practice and policies of therapeutic drug monitoring (TDM) service requesting and result reporting in Czechia and Slovakia.

Materials And Methods: All 149 laboratories that measure plasma drug concentrations were given an online questionnaire during a regular external quality assessment TDM cycle. The questionnaire consisted of 17 questions. Read More

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http://dx.doi.org/10.11613/BM.2020.020706DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7271755PMC
June 2020
2.400 Impact Factor

Errors within the total laboratory testing process, from test selection to medical decision-making - A review of causes, consequences, surveillance and solutions.

Biochem Med (Zagreb) 2020 Jun;30(2):020502

Department of Laboratory Medicine, Paracelsus Medical University, Salzburg, Austria.

Laboratory analyses are crucial for diagnosis, follow-up and treatment decisions. Since mistakes in every step of the total testing process may potentially affect patient safety, a broad knowledge and systematic assessment of laboratory errors is essential for future improvement. In this review, we aim to discuss the types and frequencies of potential errors in the total testing process, quality management options, as well as tentative solutions for improvement. Read More

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http://dx.doi.org/10.11613/BM.2020.020502DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7271754PMC

In sickness and in health: pivotal role of vitamin D.

Biochem Med (Zagreb) 2020 Jun;30(2):020501

Department of Clinical Chemistry, Sestre milosrdnice University Hospital Center, Zagreb, Croatia.

Within the last several years, frequency of vitamin D testing has multiplied substantially all over the world, since it has been shown to have an important role in many diseases and conditions. Even though liquid chromatography - tandem mass spectrometry (LC-MS/MS) has been identified as "gold standard" method for vitamin D measurement, most laboratories still use immunochemistry methods. Besides analytical problems (hydrophobicity, low circulating concentrations, ability to bind to lipids, albumins and vitamin D binding protein, presence of multiple vitamin D metabolites and variable ratios of 25(OH)D and 25(OH)D in the blood), vitamin D shows great preanalytical variability, since its concentration is drastically influenced by seasonal changes, exposure to sun, type of clothes or sun block creams. Read More

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http://dx.doi.org/10.11613/BM.2020.020501DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7271749PMC

Copyright violation of predators in scientific publishing - Biochemia Medica's harmful experience and proposed solution.

Biochem Med (Zagreb) 2020 Jun;30(2):020201

Clinical Department for Laboratory Diagnostics, Clinical Hospital Centre Rijeka, Rijeka, Croatia.

is an open access journal that does not charge manuscript processing or publishing. All editorial staff are continuously educated and directed to follow the highest ethical and scholarly publishing standards in all steps of the manuscript processing. They are all laboratory medicine professionals, who apart from their regular jobs, are in charge of different phases in Journal processing as volunteers. Read More

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http://dx.doi.org/10.11613/BM.2020.020201DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7271751PMC

Dark brown serum and plasma samples: a case report.

Biochem Med (Zagreb) 2020 Jun 15;30(2):021002. Epub 2020 Apr 15.

Department of medical laboratory diagnostics, University Hospital "Sveti Duh", Zagreb, Croatia.

This case report describes occurrence of unusual, dark brown coloration of citrate plasma and serum samples in a female 68 years old patient admitted into Emergency department (ED). Patient complained of nausea and vomiting, fever up to 38.9°C, colicky pain in abdomen, diminished urinary output and yellowish skin tone. Read More

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http://dx.doi.org/10.11613/BM.2020.021002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7138003PMC

Case report of spuriously low sodium and calcium in a 36-year-old male in primary care.

Biochem Med (Zagreb) 2020 Jun 15;30(2):021001. Epub 2020 Apr 15.

Department of Clinical Biochemistry, Cork University Hospital, Wilton, Cork, Republic of Ireland.

An unseparated serum specimen for a 36-year-old male was received from primary care. The specimen arrived in the laboratory at Cork University Hospital one day after collection, as documented on the paper request card, and was promptly centrifuged. Analysis was delayed for three days due to operational constraints and serum indices were run at the same time as the biochemical analyses. Read More

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http://dx.doi.org/10.11613/BM.2020.021001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7138000PMC

Simple thrombin-based method for eliminating fibrinogen interference in serum protein electrophoresis of haemodialysed patients.

Biochem Med (Zagreb) 2020 Jun 15;30(2):020705. Epub 2020 Apr 15.

Department of Laboratory Diagnostics, University Hospital Centre Zagreb, Zagreb, Croatia.

Introduction: Serum samples of haemodialysed patients collected through vascular access devices, central venous catheter (CVC) can contain residual heparin, which can cause incomplete clotting and consequently fibrinogen interference in serum protein electrophoresis (SPE). We hypothesized that this problem may be overcome by addition of thrombin and aimed to find a simple thrombin-based method for fibrinogen interference removal.

Materials And Methods: Blood samples of 51 haemodialysed patients with CVC were drawn through catheter into Clot Activator Tube (CAT) and Rapid Serum Tube Thrombin (RST) vacutainers (Becton Dickinson, New Jersey, USA) following the routine hospital protocols and analysed with gel-electrophoresis (Sebia, Lisses, France). Read More

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http://dx.doi.org/10.11613/BM.2020.020705DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7138005PMC

Changes in error rates in the Australian key incident monitoring and management system program.

Biochem Med (Zagreb) 2020 Jun 15;30(2):020704. Epub 2020 Apr 15.

Royal College of Pathologists of Australasia Quality Assurance Programs (RCPAQAP), Key Incident Monitoring and Management System program (KIMMS), Sydney, Australia.

Introduction: The Key incident monitoring and management system program (KIMMS) program collects data for 19 quality indicators (QIs) from Australian medical laboratories. This paper aims to review the data submitted to see whether the number of errors with a higher risk priority number (RPN) have been reduced in preference to those with a lower RPN, and to calculate the cost of these errors.

Materials And Methods: Data for QIs from 60 laboratories collected through the KIMMS program from 2015 until 2018 were retrospectively reviewed. Read More

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http://dx.doi.org/10.11613/BM.2020.020704DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7138001PMC

Analytical performance assessment and improvement by means of the Failure mode and effect analysis (FMEA).

Biochem Med (Zagreb) 2020 Jun 15;30(2):020703. Epub 2020 Apr 15.

Quality Department, Biomedical Diagnostic Center, Hospital Clínic of Barcelona, Barcelona, Spain.

Introduction: Laboratories minimize risks through quality control but analytical errors still occur. Risk management can improve the quality of processes and increase patient safety. This study aims to use the failure mode and effect analysis (FMEA) to assess the analytical performance and measure the effectiveness of the risk mitigation actions implemented. Read More

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http://dx.doi.org/10.11613/BM.2020.020703DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7137999PMC

General position of Croatian medical biochemistry laboratories on autovalidation: survey of the Working Group for Post-analytics of the Croatian Society of Medical Biochemistry and Laboratory Medicine.

Biochem Med (Zagreb) 2020 Jun 15;30(2):020702. Epub 2020 Apr 15.

Working Group for Post-analytics, Croatian Society of Medical Biochemistry and Laboratory Medicine, Zagreb, Croatia.

Introduction: Autovalidation (AV) is an algorithm based on predefined rules designed, among others, to automate and standardize the postanalytical phase of laboratory work. The aim of this study was to examine the overall opinion of Croatian medical biochemistry laboratories regarding various aspects of AV.

Material And Methods: This retrospective study is an analysis of the responses of a survey about AV comprised of 18 questions, as part of Module 10 ("Postanalytical phase of laboratory testing") of national External Quality Assessment program, administered by the Croatian Centre for Quality Assessment in Laboratory Medicine. Read More

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http://dx.doi.org/10.11613/BM.2020.020702DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7138006PMC
June 2020
2.400 Impact Factor

Long-term stability of clinically relevant chemistry analytes in pleural and peritoneal fluid.

Biochem Med (Zagreb) 2020 Jun 15;30(2):020701. Epub 2020 Apr 15.

Department of Clinical Chemistry, Sestre milosrdnice University Hospital Centre, Zagreb, Croatia.

Introduction: Our aim was to investigate the stability of clinically relevant analytes in pleural and peritoneal fluids stored in variable time periods and variable storage temperatures prior to analysis.

Materials And Methods: Baseline total proteins (TP), albumin (ALB), lactate dehydrogenase (LD), cholesterol (CHOL), triglycerides (TRIG), creatinine (CREA), urea, glucose and amylase (AMY) were measured using standard methods in residual samples from 29 pleural and 12 peritoneal fluids referred to our laboratory. Aliquots were stored for 6 hours at room temperature (RT); 3, 7, 14 and 30 days at - 20°C. Read More

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http://dx.doi.org/10.11613/BM.2020.020701DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7138007PMC

The top-down approach to measurement uncertainty: which formula should we use in laboratory medicine?

Biochem Med (Zagreb) 2020 Jun 15;30(2):020101. Epub 2020 Apr 15.

Institute of Clinical Chemistry and Biochemistry, University Medical Centre Ljubljana, Ljubljana, Slovenia.

Introduction: By quantifying the measurement uncertainty (MU), both the laboratory and the physician can have an objective estimate of the results' quality. There is significant flexibility on how to determine the MU in laboratory medicine and different approaches have been proposed by Nordtest, Eurolab and Cofrac to obtain the data and apply them in formulas. The purpose of this study is to compare three different top-down approaches for the estimation of the MU and to suggest which of these approaches could be the most suitable choice for routine use in clinical laboratories. Read More

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http://dx.doi.org/10.11613/BM.2020.020101DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7138004PMC

The effect of jet-lag on serum concentrations of thyroid stimulating hormone and prolactin: A case report.

Biochem Med (Zagreb) 2020 Feb;30(1):011003

Duzen Laboratories Group, Ankara, Turkey.

Introduction: This case report is about the importance of sleeping status for analysis of thyroid hormone stimulating hormone (TSH) and prolactin (PRL) which arose from discordant results of a patient who was referred for serum TSH and PRL testing within 12-hour period after an intercontinental flight.

Case Description: An adult male patient was admitted to our laboratory for serum TSH and PRL tests and came back questioning the accuracy of his previous results.

Further Investigations: A new analysis with a new sample was offered. Read More

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http://dx.doi.org/10.11613/BM.2020.011003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6999183PMC
February 2020

Six Sigma revisited: We need evidence to include a 1.5 SD shift in the extraanalytical phase of the total testing process.

Biochem Med (Zagreb) 2020 Feb;30(1):010901

Department of Physiology and Pharmacology, Sapienza University of Rome, Rome, Italy.

The Six Sigma methodology has been widely implemented in industry, healthcare, and laboratory medicine since the mid-1980s. The performance of a process is evaluated by the sigma metric (SM), and 6 sigma represents world class performance, which implies that only 3.4 or less defects (or errors) million opportunities (DPMO) are expected to occur. Read More

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http://dx.doi.org/10.11613/BM.2020.010901DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6999184PMC
February 2020

Title does matter: a cross-sectional study of 30 journals in the Medical Laboratory Technology category.

Biochem Med (Zagreb) 2020 Feb;30(1):010708

Department of Medical Laboratory Diagnostics, University Hospital Centre Split, Split, Croatia.

Introduction: First impression on potential readers is created by the title; therefore, authors should give importance to the title structure. The aim of this study was to establish whether articles created by a smaller number of authors and with shorter, descriptive or declarative titles gain more citations and whether article title length and number of authors correlate to the number of citations.

Material And Methods: A cross-sectional study on article citation data for 30 scientific journals published in 2016 in Medical Laboratory Technology field according to Web of Science database was conducted. Read More

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http://dx.doi.org/10.11613/BM.2020.010708DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6999185PMC
February 2020

Test validation, method comparison and reference range for the measurement of β-hydroxybutyrate in peripheral blood samples.

Biochem Med (Zagreb) 2020 Feb;30(1):010707

Central Laboratory, University Hospital Halle, Halle (Saale), Germany.

Introduction: The measurement of β-hydroxybutyrate (βOHB) concentrations is a corner stone of the diagnosis of diabetic ketoacidosis and other ketonic states. The aim of this study was to perform a validation of a peripheral blood βOHB assay (Randox) on a Roche cobas c502 analyser and to establish a βOHB reference range for the validated assay.

Materials And Methods: Precision, linearity and limit of detection and blank (LoD, LoB) were determined according to Clinical and Laboratory Standards Institute (CLSI) EP05-A3, EP 06-A and EP17-A2 guidelines, using commercial control material and residual patient sample pools. Read More

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http://dx.doi.org/10.11613/BM.2020.010707DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6999181PMC
February 2020

Analytical verification and comparison of chromogenic assays for dabigatran, rivaroxaban and apixaban determination on BCSXP and STA Compact Max analysers.

Biochem Med (Zagreb) 2020 Feb;30(1):010706

Department of Clinical Chemistry, Sestre milosrdnice University Hospital Center, Zagreb, Croatia.

Introduction: The aim of the study was to perform analytical verification and comparison of chromogenic assays for determination of dabigatran, rivaroxaban and apixaban concentration on BCSXP and STA Compact Max analysers.

Materials And Methods: Precision, linearity, measurement uncertainty estimation and determination of limit of blank, limit of determination and limit of quantification were calculated. Analytical performance specifications were set according to manufacturer specifications and literature data on between laboratory variability. Read More

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http://dx.doi.org/10.11613/BM.2020.010706DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6999186PMC
February 2020

Statistical distributions commonly used in measurement uncertainty in laboratory medicine.

Biochem Med (Zagreb) 2020 Feb;30(1):010101

Department of Clinical Chemistry and Hematology, Zuyderland Medical Centre, Sittard/Heerlen, The Netherlands.

Uncertainty is an inseparable part of all types of measurement. Recently, the International Organization for Standardization (ISO) released a new standard (ISO 20914) on how to calculate measurement uncertainty (MU) in laboratory medicine. This standard can be regarded as the beginning of a new era in laboratory medicine. Read More

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http://dx.doi.org/10.11613/BM.2020.010101DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6999182PMC
February 2020

Unexpected abnormal coagulation test results in a 2-year-old child: A case report.

Biochem Med (Zagreb) 2020 Feb 15;30(1):011002. Epub 2019 Dec 15.

Department of transfusion medicine, General Hospital Pula, Pula, Croatia.

Rejection of the sample with repeated blood withdrawal is always an unwanted consequence of sample nonconformity and preanalytical errors, especially in the most vulnerable population - children. Here is presented a case with unexpected abnormal coagulation test results in a 2-year-old child with no previously documented coagulation disorder. Child is planned for tympanostomy tubes removal under the anaesthesia driven procedure, and preoperative coagulation tests revealed prolonged prothrombin time, activated partial thromboplastin time and thrombin time, with fibrinogen and antithrombin within reference intervals. Read More

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http://dx.doi.org/10.11613/BM.2020.011002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6904964PMC
February 2020

High anion gap metabolic acidosis caused by D-lactate: mind the time of blood collection.

Biochem Med (Zagreb) 2020 Feb 15;30(1):011001. Epub 2019 Dec 15.

Clinical Department of Laboratory Medicine, UZ Leuven, Leuven, Belgium.

Introduction: D-lactic acidosis is an uncommon cause of high anion gap acidosis.

Materials And Methods: A 35-year old woman was admitted to the emergency room with somnolence, drowsiness, dizziness, incoherent speech and drunk appearance. Her past medical history included a Roux-en-Y bypass. Read More

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http://dx.doi.org/10.11613/BM.2020.011001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6904971PMC
February 2020

An unconscious man with profound drug-induced hypoglycaemia.

Biochem Med (Zagreb) 2020 Feb 15;30(1):010802. Epub 2019 Dec 15.

Laboratory Medicine, University Hospitals Leuven; Department of cardiovascular Medicine, University of Leuven, Leuven, Belgium.

Introduction: Hypoglycaemia has been reported as an unusual complication of tramadol use and in a few cases of tramadol poisoning, but the exact mechanism is not known.

Case Description: An ambulance crew was dispatched to an unconscious 46-year old man. A glucometer point-of-care measurement revealed a profound hypoglycaemia (1. Read More

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http://dx.doi.org/10.11613/BM.2020.010802DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6904963PMC
February 2020

A case of IgE myeloma transformed into IgE-producing plasma cell leukaemia.

Biochem Med (Zagreb) 2020 Feb 15;30(1):010801. Epub 2019 Dec 15.

Department of Biochemistry and Pharmaco-Toxicology, Martinique University Hospital, Fort-de-France, France.

This is a case report of a challenging diagnosis of IgE monoclonal gammopathy of undetermined significance, which transformed into myeloma, then transformed into IgE-producing plasma cell leukaemia in a 71-year-old male who was followed in Brest, France, from 2015 to 2019. The IgE-producing variant is the rarest sub-type of multiple myeloma, and plasma cell leukaemia is considered to be the rarest and the most aggressive of human monoclonal gammopathies. In November 2015, hypogammaglobulinemia was detected during a systematic check-up. Read More

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http://dx.doi.org/10.11613/BM.2020.010801DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6904968PMC
February 2020

Knowledge and awareness of iodine intake - survey among Croatian women of reproductive age.

Biochem Med (Zagreb) 2020 Feb 15;30(1):010705. Epub 2019 Dec 15.

Department of Clinical Chemistry, Sestre milosrdnice University Hospital Center, Zagreb, Croatia.

Introduction: Appropriate iodine intake is important for the entire population, especially in fertile women due to decisive role of thyroid hormones in normal foetal brain development. The aim of this study was to investigate knowledge on iodine role among Croatian women of reproductive age.

Materials And Methods: The survey was conducted among 378 women of reproductive age during May-September 2018. Read More

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http://dx.doi.org/10.11613/BM.2020.010705DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6904972PMC
February 2020

Preanalytical issues related to routine and diagnostic glucose tests: Results from a survey in Spain.

Biochem Med (Zagreb) 2020 Feb 15;30(1):010704. Epub 2019 Dec 15.

Spanish Society of Laboratory Medicine, Extra-analytical Quality Commission, Barcelona, Spain.

Introduction: Diabetes mellitus (DM) is one of the most prevalent diseases worldwide. The objective of this study was to find out under what preanalytical conditions routine and diagnostic glucose tests are performed across Spanish laboratories; and also what criteria are used for DM diagnosis.

Materials And Methods: An online survey was performed by the Commission on Quality Assurance in the Extra-Analytical Phase of the Spanish Society of Laboratory Medicine (SEQC-ML). Read More

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http://dx.doi.org/10.11613/BM.2020.010704DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6904967PMC
February 2020

Study of the preanalytical variables affecting the measurement of clinically relevant free-circulating microRNAs: focus on sample matrix, platelet depletion, and storage conditions.

Biochem Med (Zagreb) 2020 Feb 15;30(1):010703. Epub 2019 Dec 15.

Laboratory of Experimental Biochemistry & Molecular Biology, IRCCS Istituto ortopedico Galeazzi, Milano, Italia.

Introduction: Circulating microRNAs (miRNAs) are emerging as potential biomarkers. However, the lack of preanalytical and analytical standardization limits their use. The aim of this study was to determine the expression of different miRNAs in plasma according to different collection and storage conditions. Read More

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http://dx.doi.org/10.11613/BM.2020.010703DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6904965PMC
February 2020

Chromogenic anti-FXa assay calibrated with low molecular weight heparin in patients treated with rivaroxaban and apixaban: possibilities and limitations.

Biochem Med (Zagreb) 2020 Feb 15;30(1):010702. Epub 2019 Dec 15.

Department of Neurology, Sestre milosrdnice University Hospital Center, Zagreb, Croatia.

Introduction: Clinical application of rivaroxaban and apixaban does not require therapeutic monitoring. Commercial anti-activated factor X (anti-FXa) inhibition methods for all anti-FXa drugs are based on the same principle, so there are attempts to evaluate potential clinical application of heparin-calibrated anti-FXa assay as an alternative method for direct FXa inhibitors. We aimed to evaluate relationship between anti-FXa methods calibrated with low molecular weight heparin (LMWH) and with drug specific calibrators, and to determine whether commercial LMWH anti-FXa assay can be used to exclude the presence of clinically relevant concentrations of rivaroxaban and apixaban. Read More

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http://dx.doi.org/10.11613/BM.2020.010702DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6904970PMC
February 2020

Platelet indices in stable chronic obstructive pulmonary disease - association with inflammatory markers, comorbidities and therapy.

Biochem Med (Zagreb) 2020 Feb 15;30(1):010701. Epub 2019 Dec 15.

University of Zagreb, Faculty of Pharmacy and Biochemistry, Department of Medical Biochemistry and Haematology, Zagreb, Croatia.

Introduction: Chronic obstructive pulmonary disease (COPD) is a complex inflammatory condition that can affect haemostasis. This study aimed to determine differences in platelet-related parameters between controls and COPD subjects. The hypothesis was that platelet indices are disturbed in COPD patients, and this would be accompanied by increased C-reactive protein (CRP), fibrinogen (Fbg) and white blood cells (WBC). Read More

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http://dx.doi.org/10.11613/BM.2020.010701DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6904969PMC
February 2020

Laboratory testing of extravascular body fluids: National recommendations on behalf of the Croatian Society of Medical Biochemistry and Laboratory Medicine. Part I - Serous fluids.

Biochem Med (Zagreb) 2020 Feb 15;30(1):010502. Epub 2019 Dec 15.

Croatian Society of Medical Biochemistry and Laboratory Medicine, Working group for extravascular body fluid samples.

Extravascular body fluids (EBF) analysis can provide useful information in the differential diagnosis of conditions that caused their accumulation. Their unique nature and particular requirements accompanying EBF analysis need to be recognized in order to minimize possible negative implications on patient safety. This recommendation was prepared by the members of the Working group for extravascular body fluid samples (WG EBFS). Read More

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http://dx.doi.org/10.11613/BM.2020.010502DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6904973PMC
February 2020

Integrated diagnostics: the future of laboratory medicine?

Biochem Med (Zagreb) 2020 Feb 15;30(1):010501. Epub 2019 Dec 15.

Department of Laboratory Medicine, University Hospital of Padova, Padova, Italy.

The current scenario of and diagnostics can be summarized using the "silo metaphor", where laboratory medicine, pathology and radiology are three conceptually separated diagnostic disciplines, which will increasingly share many comparable features. The substantial progresses in our understanding of biochemical-biological interplays that characterize many human diseases, coupled with extraordinary technical advances, are now generating important multidisciplinary convergences, leading the way to a new frontier, called integrated diagnostics. This new discipline, which is currently defined as convergence of imaging, pathology and laboratory tests with advanced information technology, has an enormous potential for revolutionizing diagnosis and therapeutic management of human diseases, including those causing the largest number of worldwide deaths ( cardiovascular disease, cancer and infectious diseases). Read More

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http://dx.doi.org/10.11613/BM.2020.010501DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6904966PMC
February 2020

spheroplasts in a Croatian patient misclassified by two urine sediment analysers as erythrocytes: case report.

Biochem Med (Zagreb) 2019 Oct;29(3):030801

Department of Medical Laboratory Diagnostics, University Hospital "Sveti Duh", Zagreb, Croatia.

Introduction: It has already been reported that subinhibitory concentrations of β-lactam antibiotics can cause abnormal changes of bacterial forms, such as spheroplasts. Herein we report a case of Croatian male patient with spheroplasts present in urine after treatment with tazobactam, on the tenth day of hospitalization. The aim of this report is to emphasize the inability of imaging based automated urine analysers to recognize some relatively uncommon forms of bacterial presentation in urine sediment. Read More

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http://dx.doi.org/10.11613/BM.2019.030801DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6784421PMC
October 2019

Clinician's opinion about critical risk results proposed by the Croatian Chamber of Medical Biochemists: a survey in one Croatian tertiary hospital.

Biochem Med (Zagreb) 2019 Oct;29(3):030711

Department of Medical Laboratory Diagnostics, University Hospital "Sveti Duh", Zagreb, Croatia.

Introduction: It has been recommended that each laboratory modify their critical result reporting practices to reflect the clinical needs of their patient populations. The aim of this survey was to assess how well critical laboratory values defined by the Croatian Chamber of Medical Biochemists (CCMB) correspond to the needs of the physicians at University hospital "Sveti Duh" (Zagreb, Croatia).

Materials And Methods: We conducted a survey among physicians from five departments in our hospital. Read More

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http://dx.doi.org/10.11613/BM.2019.030711DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6784420PMC
October 2019

Optimizing moving average control procedures for small-volume laboratories: can it be done?

Biochem Med (Zagreb) 2019 Oct;29(3):030710

Department of Medical Biochemistry, University of Belgrade, Faculty of Pharmacy, Belgrade, Serbia.

Introduction: Moving average (MA) means calculating the average value from a set of patient results and further using that value for analytical quality control purposes. The aim of this study was to examine whether the selection, optimization and validation of MA procedures can be performed using the already described bias detection simulation method and whether it is possible to select appropriate MA procedures for a laboratory with a small daily testing volume.

Materials And Methods: The study was done on four analytes: creatinine, potassium, sodium and albumin. Read More

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http://dx.doi.org/10.11613/BM.2019.030710DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6784424PMC
October 2019

Verification study of free light chains assays on reagent-optimized analysers.

Biochem Med (Zagreb) 2019 Oct;29(3):030709

Department of Laboratory Diagnostics, University Hospital Centre Zagreb, Zagreb, Croatia.

Introduction: Our aim was to compare analytical specifications of two assays (monoclonal vs. polyclonal) for free light chains (FLCs) quantification optimized for two different analytical platforms, nephelometer ProSpec (Siemens, Erlangen, Germany) and turbidimetric analyser Optilite (The Binding Site, Birmingham, UK).

Materials And Methods: The evaluation included verification of the precision, repeatability and reproducibility, estimation of accuracy and method comparison study with 37 serum samples of haematological patients. Read More

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http://dx.doi.org/10.11613/BM.2019.030709DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6784423PMC
October 2019

Time-dependent variation of ionized calcium in serum samples.

Biochem Med (Zagreb) 2019 Oct;29(3):030708

Department of Laboratory Diagnostics, Dubrovnik General Hospital, Dubrovnik, Croatia.

Introduction: The aim of this study was to compare ionized calcium (iCa) concentrations in arterial heparinized blood and venous serum and to investigate time-dependent variation of iCa in serum samples centrifuged and analysed at different times.

Materials And Methods: Ionized calcium was measured (N = 25) in arterial blood within 20 min after puncture, and in serum within 10 min after centrifugation conducted 30 min after sampling. Effect of time between sampling and centrifugation was examined in three tubes (N = 30) centrifuged 15, 30 and 60 min after sampling, and analysed within 10 min. Read More

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http://dx.doi.org/10.11613/BM.2019.030708DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6784427PMC
October 2019

Classical pathway activity C3c, C4 and C1-inhibitor protein reference intervals determination in EDTA plasma.

Biochem Med (Zagreb) 2019 Oct;29(3):030707

Department of Immunology, Lille University Hospital, Lille, France.

Introduction: Reference intervals (RIs) for complement assays in EDTA plasma samples have not previously been published. The objectives of the present study were to validate and/or determine RIs for classical pathway (CP50) activity and C3c, C4 and C1 inhibitor protein (C1INH) assays and to assess the need for age-specific RIs in EDTA plasma.

Materials And Methods: We retrospectively evaluated a cohort of 387 patients attending our university hospital and known to be free of complement-modifying diseases. Read More

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http://dx.doi.org/10.11613/BM.2019.030707DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6784422PMC
October 2019
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Rapid serum tube technology overcomes problems associated with use of anticoagulants.

Biochem Med (Zagreb) 2019 Oct;29(3):030706

Faculty of Medicine, University of Queensland, Translational Research Institute, Brisbane, Australia.

Introduction: Failure to obtain complete blood clotting in serum is a common laboratory problem. Our aim was to determine whether snake proth-rombin activators are effective in clotting blood and producing quality serum for analyte measurement in anticoagulated patients.

Materials And Methods: Whole blood clotting was studied in a total of 64 blood samples (41 controls, 20 Warfarin patients, 3 anticoagulated patients using snake venom prothrombin activator (OsPA)) with plain tubes. Read More

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http://dx.doi.org/10.11613/BM.2019.030706DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6784418PMC
October 2019

Effectiveness of minimum retesting intervals in managing repetitive laboratory testing: experience from a Croatian university hospital.

Biochem Med (Zagreb) 2019 Oct;29(3):030705

Department of Laboratory Diagnostics, University Hospital Center Zagreb, Zagreb, Croatia.

Introduction: Inappropriate laboratory retesting can be addressed by implementing minimum retesting intervals (MRI). The aim of our study was to assess the effectiveness of the implemented MRI protocol for inpatients.

Materials And Methods: Minimum retesting intervals were applied for 53 laboratory tests. Read More

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http://dx.doi.org/10.11613/BM.2019.030705DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6784426PMC
October 2019

Confidence interval of percentiles in skewed distribution: The importance of the actual coverage probability in practical quality applications for laboratory medicine.

Biochem Med (Zagreb) 2019 Oct;29(3):030101

Department of Physiology and Pharmacology, Sapienza University of Rome, Rome, Italy.

Introduction: Quality indicators (QI) based on percentiles are widely used for managing quality in laboratory medicine nowadays. Due to their statistical nature, their estimation is affected by sampling so they should be always presented together with the confidence interval (CI). Since no methodological recommendation has been issued to date, our aim was investigating the suitability of the parametric method (LP-CI), the non-parametric binomial (NP-CI) and bootstrap (BCa-CI) procedures for the CI estimation of 2. Read More

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http://dx.doi.org/10.11613/BM.2019.030101DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6784425PMC
October 2019

Flushing of an intravenous catheter: a cause for unreliable laboratory results.

Biochem Med (Zagreb) 2019 Oct 5;29(3):031001. Epub 2019 Aug 5.

Department of Clinical Chemistry, Amsterdam UMC, University of Amsterdam, Amsterdam, Netherlands.

Introduction: Phlebotomy is an error-prone process in which mistakes are difficult to reveal. This case report describes the effect on laboratory results originating from a blood sample collected in close proximity to an intravenous catheter.

Materials And Methods: A 69-year-old male patient was referred to the Emergency department where pneumonia was suspected. Read More

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http://dx.doi.org/10.11613/BM.2019.031001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6610670PMC
October 2019
10 Reads

Assessment of the degree of adherence of medical laboratories to KDIGO 2012 guideline for evaluation and management of CKD in Czechia and Slovakia.

Biochem Med (Zagreb) 2019 Oct 5;29(3):030704. Epub 2019 Aug 5.

SEKK, spol. s.r.o., Pardubice, Czech Republic.

Introduction: The aim of the study is to assess the degree of adherence of medical laboratories to Kidney Disease Improving Global Outcomes (KDIGO) 2012 Clinical Practice Guideline for the Evaluation and Management of Chronic Kidney Disease (CKD) in laboratory practice in Czechia and Slovakia.

Materials And Methods: An electronic questionnaire on adherence to KDIGO 2012 guideline was designed by an external quality assessment (EQA) provider SEKK spol. s. Read More

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http://dx.doi.org/10.11613/BM.2019.030704DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6610673PMC
October 2019
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2.400 Impact Factor

Assessment of a laboratory critical risk result notification protocol in a tertiary care hospital and their use in clinical decision making.

Biochem Med (Zagreb) 2019 Oct 5;29(3):030703. Epub 2019 Aug 5.

Department of Laboratory Medicine, Hospital Universitari Son Espases, Palma, Spain.

Introduction: Communication of laboratory critical risk results is essential for patient safety, as it allows early decision making. Our aims were: 1) to retrospectively evaluate the current protocol for telephone notification of critical risk results in terms of rates, efficiency and recipient satisfaction, 2) to assess their use in clinical decision making and 3) to suggest alternative tools for a better assessment of notification protocols.

Materials And Methods: The biochemical critical risk result notifications reported during 12 months by routine and STAT laboratories in a tertiary care hospital were reviewed. Read More

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http://dx.doi.org/10.11613/BM.2019.030703DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6610674PMC
October 2019
6 Reads