394 results match your criteria Biocell[Journal]


Meta-analysis of the cell cycle related C12orf48.

Authors:
Lokman Varisli

Biocell 2013 Apr;37(1):11-6

Harran University, Art and Science Faculty, Department of Biology, Osmanbey Campus, Sanliurfa, Turkey.

The cell cycle is a conserved process from yeast to mammals and focuses on mechanisms that regulate the timing and frequency of DNA replication and cell division. The temporal and spatial expression of the genes is tightly regulated to ensure accurate replication and transmission of DNA to daughter cells during the cycle. Although the genes involved in interphase are well studied, most of the genes which are involved in mitotic events still remain unidentified. Read More

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High passage numbers induce resistance to apoptosis in C2C12 muscle cells.

Biocell 2013 Apr;37(1):1-9

Departamento de Biología, Bioquímica y Farmacia, Universidad Nacional del Sur. San Juan 670, 8000 Bahía Blanca, Argentina.

Cell lines with high passage numbers exhibit alterations in cell morphology and functions. In the present work, C2C12 skeletal muscle cells with either low (< 20) or high (> 60) passage numbers (identified as 1-C2C12 or h-C2C12, respectively) were used to investigate the apoptotic response to H2O2 as a function of culture age h-C2C12. We found that older cultures (h-C2C12 group) were depleted of mitochondrial DNA (mtDNA). Read More

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April 2013
3 Reads

Autophagy, apoptosis and organelle features during cell exposure to cadmium.

Biocell 2013 Aug;37(2):45-54

Universidade Estadual do Norte Fluminense, Centro de Biociências e Biotecnologia, Laboratório de Biologia Celular e Tecidual, Campos dos Goytacazes, RJ 28013-602, Brasil.

Cadmium (Cd) induces several effects in different tissues, but our knowledge of the toxic effects on organelles is insufficient. To observe the progression of Cd effects on organelle structure and function, HuH-7 cells (human hepatic carcinoma cell line) were exposed to CdCl2 in increasing concentrations (1 microM - 20 microM) and exposure times (2 h - 24 h). During Cd treatment, the cells exhibited a progressive decrease in viability that was both time- and dose-dependent. Read More

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Creatine supplementation in trained rats causes changes in myenteric neurons and intestinal wall morphometry.

Biocell 2013 Aug;37(2):37-43

Universidade Estadual de Maringá, Avenida Colombo 5790, Bloco H79, sala 108 Maringá, Paraná 87020 900, Brazil.

Creatine is widely used by athletes as an ergogenic resource. The aim of this study was to evaluate the influence of creatine supplementation on the duodenum of rats submitted to physical training. The number and myenteric neuronal cell bodies as well mucosal and muscular tunic morphometry were evaluated. Read More

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Confocal laser scanning microscopy and immunohistochemistry of cerebellar Lugaro cells.

Biocell 2013 Aug;37(2):29-36

Biological Research Institute "Drs. Orlando Castejón and Haydée Viloria de Castejón", Faculty of Medicine, Zulia University, Maracaibo, Venezuela.

The present paper shows by means of confocal laser scanning microscopy the immunoreactivity of rat cerebellar Lugaro cells for calbindin, synapsin-I, PSD-95, GluR1, CaMKII alpha, and N-cadherin. Lugaro cells were easily characterized by their location beneath Purkinje cells. Calbindin revealed immunoreactivity in the cell body, and the axonal and dendritic processes. Read More

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Post-treatment with plant extracts used in Brazilian folk medicine caused a partial reversal of the antiproliferative effect of glyphosate in the Allium cepa test.

Biocell 2013 Aug;37(2):23-8

Department of Biology, Centro de Ciências Naturais e Exatas (CCNE), Universidade Federal de Santa Maria (UFSM), Avenida Roraima, no 1000, Cep. 97105-900, Camobi, Santa Maria, Rio Grande do Sul, Brasil.

Species of the genus Psychotria are used for multiple purposes in Brazilian folk medicine, either as water infusions, baths or poultices. This study was aimed to evaluate the genotoxic and antiproliferative effects of infusions of Psychotria brachypoda and P. birotula on the Allium cepa test. Read More

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August 2013
5 Reads

Age related interaction of dopamine and serotonin synthesis in striatal synaptosomes.

Biocell 2013 Aug;37(2):17-21

Medical Plant Research Center, Shahre Kord University of Medical Sciences, Iran.

Tyrosine hydroxylase and tryptophan hydroxylase are key rate limiting enzymes in the biosynthesis of dopamine and serotonin, respectively. Since both enzymes are active in striatum, and affected by age, this study was undertaken to investigate interaction between dopamine and serotonin synthesis in brain striatal synaptosomes of aging rat. Male Wistar rats (3 and 30 month old) were killed by decapitation and brain striatal synaptosomes were prepared by discontinuous Ficoll/sucrose gradient technique. Read More

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August 2013
1 Read
3 Citations
0.571 Impact Factor

Pollen viability of Polygala paniculata L. (Polygalaceae) using different staining methods.

Biocell 2012 Dec;36(3):143-5

Laboratory of Cytogenetics and Genotoxicity, Department of Biology, Centro de Ciências Naturais e Exatas (CCNE), Universidade Federal de Santa Maria (UFSM), Avenida Roraima, no 1000, Cep. 97105-900, Camobi, Santa Maria, Rio Grande do Sul, Brasil.

Polygala paniculata L. is a medicinal plant that grows in the Brazilian Atlantic coast, known as 'barba-de-São-João', 'barba-de-bode', 'vassourinha branca', and 'mimosa'. In this study, pollen viability was estimated by three different staining methods: 2% acetic orcein, 2% acetic carmine, and Alexander's stain. Read More

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December 2012
34 Reads

Structural analysis of flagellar axonemes from inner arm dynein knockdown strains of Trypanosoma brucei.

Biocell 2012 Dec;36(3):133-41

Department of Biology, Amherst College, Amherst, MA, USA.

Trypanosoma brucei is a protozoan flagellate that causes African sleeping sickness. Flagellar function in this organism is critical for life cycle progression and pathogenesis, however the regulation of flagellar motility is not well understood. The flagellar axoneme produces a complex beat through the precisely coordinated firing of many proteins, including multiple dynein motors. Read More

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December 2012
6 Reads

A likely role for a novel PH-domain containing protein, PEPP2, in connecting membrane and cytoskeleton.

Biocell 2012 Dec;36(3):127-32

Department of Biology, School of Life Science and Technology, Jinan University, Guangzhou, China.

PH domains (pleckstrin homology) are well known to bind membrane phosphoinositides with different specificities and direct PH domain-containing proteins to discrete subcellular apartments with assistances of alternative binding partners. PH domain-containing proteins are found to be involved in a wide range of cellular events, including signalling, cytoskeleton rearrangement and vesicular trafficking. Here we showed that a novel PH domain-containing protein, PEPP2, displayed moderate phosphoinositide binding specificity. Read More

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December 2012
4 Reads

Knockdown of apoptosis-inducing factor disrupts function of respiratory complex I.

Biocell 2012 Dec;36(3):121-6

Centre for Biomedical Image Analysis, Faculty of Informatics, Masaryk University, Botanická 68a, Brno 60200, Czech Republic.

Recent findings suggest that apoptotic protein apoptosis-inducing factor (AIF) may also play an important non-apoptotic function inside mitochondria. AIF was proposed to be an important component of respiratory chain complex I that is the major producer of superoxide radical. The possible role of AIF is still controversial. Read More

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December 2012
5 Reads

Mechanisms involved in the cytotoxic effects of berberine on human colon cancer HCT-8 cells.

Biocell 2012 Dec;36(3):113-20

College of Pharmacy, Dalian Medical University, 9 Western Lvshun South Road, Dalian 116044, China.

Berberine, a constituent of some traditional Chinese medicinal plants, has been reported to have cytotoxicity effects on different human cancer cell lines. There is no available information about the effects and mechanism of action of berberine on human colon cancer cell line HCT-8. In this paper, the cytotoxicity ofberberine on HCT-8 cancer cells was investigated by MTT assay, fluorescence microscopy and flow cytometry analysis. Read More

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http://www.scielo.org.ar/pdf/biocell/v36n3/v36n3a03.pdf
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December 2012
42 Reads

Reproductive performance of the Mesa silverside (Chirostoma jordani Woolman, 1894) under natural and controlled photoperiods.

Biocell 2012 Dec;36(3):105-11

Posta Zootécnica, Departamento de Zootecnia, Centro de Ciencias Agropecuarias, Universidad Autónoma de Aguascalientes, Jesús María, Aguascalientes, México.

Chirostoma jordani is a native annual species inhabiting lacustrine waters of the Central Mexico Plateau. It is widely distributed and is currently facing high environmental pressures. Five experiments were performed to study the reproductive performance of this species. Read More

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December 2012

Effects of rotational culture on morphology, nitric oxide production and cell cycle of endothelial cells.

Biocell 2012 Dec;36(3):97-103

Key Laboratory of Biorheological Science and Technology, Chongqing University, Ministry of Education, Chongqing 400044, China.

Devices for the rotational culture of cells and the study of biological reactions have been widely applied in tissue engineering. However, there are few reports exploring the effects of rotational culture on cell morphology, nitric oxide (NO) production, and cell cycle of the endothelial cells from human umbilical vein on the stent surface. This study focuses on these parameters after the cells are seeded on the stents. Read More

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December 2012
3 Reads

Modulation of IL-10/IL-10R expression by mafosfamide, a derivative of 4-hydroxycyclophosphamide, in a rat B-cell lymphoma.

Biocell 2012 Aug;36(2):91-5

Instituto de Genética Experimental, Facultad de Ciencias Médicas, Universidad Nacional de Rosario, Santa Fe 3100, (2000) Rosario, Argentina.

We have already shown that IL-10 plays an important role in immunosuppression and metastatic dissemination in the rat B-cell lymphoma L-TACB model. It was suggested that the up-regulation of IL-10 production and IL-10 receptor (IL-10R) expression would be part of the transition from primary tumor to metastatic phenotype and that IL-10, besides its immunosuppressive activity, may act as a growth factor for metastatic L-TACB cells. The treatment of L-TACB-bearing rats with a single low-dose cyclophosphamide decreased IL-10 production, reverted immunosuppression and induced the immunologic rejection of tumor metastasis without any effect on primary tumor growth. Read More

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Histological analysis of pollen-pistil interactions in sour passion fruit plants (Passiflora edulis Sims).

Biocell 2012 Aug;36(2):83-90

Laboratório de Melhoramento Genético Vegetal, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Av. Alberto Lamego 2000, Horto, 28013-602, Campos dos Goytacazes, RJ, Brazil.

The success of sexual plant reproduction is directly influenced by specific interactions between the pollen and pistil. Light, fluorescence and scanning electron microscopy techniques were used to evaluate the steps of pollination in sour passion fruit plants (Passiflora edulis Sims). In the compatible interaction, pollen tubes grow through stigma projections towards the ovary. Read More

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August 2012
9 Reads

Human umbilical artery smooth muscle exhibits a 2-APB-sensitive capacitative contractile response evoked by vasoactive substances and expresses mRNAs for STIM, Orai and TRPC channels.

Biocell 2012 Aug;36(2):73-81

GINFIV--Grupo de Investigación en Fisiologia Vascular, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Argentina.

After depletion of intracellular Ca2+ stores the capacitative response triggers an extracellular Ca2+ influx through store-operated channels (SOCs) which refills these stores. Our objective was to explore if human umbilical artery smooth muscle presented this response and if it was involved in the mechanism of serotonin- and histamine-induced contractions. Intracellular Ca2+ depletion by a Ca(2+)-free extracellular solution followed by Ca2+ readdition produced a contraction in artery rings which was inhibited by the blocker of Orai and TRPC channels 2-aminoethoxydiphenyl borate (2-APB), suggesting a capacitative response. Read More

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August 2012
1 Read

Butein imparts free radical scavenging, anti-oxidative and proapoptotic properties in the flower extracts of Butea monosperma.

Biocell 2012 Aug;36(2):63-71

Virology Group, International Centre for Genetic Engineering and Biotechnology, Aruna AsafAli Marg, New Delhi 110067, India.

The flower of Butea monosperma (Lam.) (Fabaceae) has been used in traditional Indian medicine in the treatment of many ailments including liver disorders. To understand the pharmacological basis of its beneficial effects, the extracts of dried flowers in water, methanol, butanol, ethyl acetate and acetone were evaluated for free radical scavenging and pro-apoptotic activities in cell cultures (human hepatoma Huh-7 cell line and immortalized AML-12 mouse hepatocytes). Read More

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Seasonal testicular changes in Dendropsophus minutus Peters, 1872 (Anura, Hylidae).

Biocell 2012 Aug;36(2):57-62

Universidade Federal de Mato Grosso (UFMT), Instituto de Biociências, Cuiabá, MT, Brasil.

The reproductive cycle in anurans may be either continuous or discontinuous. These differences may be connected to seasonal climate changes and/or to anthropic activity. Forty adult male individuals of the Dendropsophus minutus species were collected during one year, in the municipality of Chapada dos Guimarães (Mato Grosso, Brazil). Read More

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August 2012
1 Read

A study of chlorophyll-like and phycobilin pigments in the C endosymbiont of the apple-snail pomacea canaliculata.

Biocell 2012 Aug;36(2):47-55

Laboratorio de Fisiología (IHEM-CONICET), and Departamento de Morfología y Fisiología, Facultad de Ciencias Médicas, Universidad Nacional de Cuyo, Casilla de Correo 33, 5500 Mendoza, Argentina.

Pigments present in the brown-greenish C morph of an intracellular endosymbiont of Pomacea canaliculata were investigated. Acetone extracts of the endosymbiotic corpuscles showed an absorption spectrum similar to that of chlorophylls. Three fractions obtained from silica gel column chromatography of the acetone extracts (C(I), C(II), and C(III)), were studied by positive ion fast atom bombardment-mass spectrometry (FAB-MS) and hydrogen-nuclear magnetic resonance (H-NMR). Read More

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August 2012
2 Reads

Optimization and comparison of two different 3D culture methods to prepare cell aggregates as a bioink for organ printing.

Biocell 2012 Apr;36(1):37-45

Department of Biomedical Engineering, Amirkabir University of Technology, Tehran, Iran.

The ultimate goal of tissue engineering is to design and fabricate functional human tissues that are similar to natural cells and are capable of regeneration. Preparation of cell aggregates is one of the important steps in 3D tissue engineering technology, particularly in organ printing. Two simple methods, hanging drop (HD) and conical tube (CT) were utilized to prepare cell aggregates. Read More

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April 2012
10 Reads
1 Citation
0.571 Impact Factor

Cryopreservation of Cyrtopodium hatschbachii Pabst (Orchidaceae) immature seeds by encapsulation-dehydration.

Biocell 2012 Apr;36(1):31-6

Instituto de Botánica del Nordeste (IBONE), CONICET--UNNE, Facultad de Ciencias Agrarias (UNNE), C.C. 209, (3400) Corrientes, Argentina.

The aim of the present study was to investigate the efficiency of the encapsulation-dehydration technique for cryopreservation of Cyrtopodium hastchbachii Pabst seeds. Immature seeds of this species were cryopreserved by an encapsulation-dehydration technique. Seeds of five immature pods, 120 days after pollination, were encapsulated in 3% calcium alginate matrix and pretreated in liquid medium supplemented with 0. Read More

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April 2012
43 Reads

Correlative microscopy of Purkinje cells.

Biocell 2012 Apr;36(1):1-29

Instituto de Investigaciones Biologicas "Drs. Orlando Castejón and Haydee Viloria de Castejón", Facultad de Medicina, Universidad del Zulia, Maracaibo, Venezuela.

The Purkinje cell and their synaptic contacts have been described using (1) light microsocopy, (2) transmission and scanning electron microscopy, and freeze etching technique, (3) conventional and field emission scanning electron microscopy and cryofracture methods, (4) confocal laser scanning microscopy using intravital stain FM64, and (5) immunocytochemical techniques for Synapsin-I, PSD9-5, GluR1 subunit of AMPA receptors, N-cadherin, and CamKII alpha. The outer surface and inner content of plasma membrane, cell organelles, cytoskeleton, nucleus, dendritic and axonal processes have been exposed and analyzed in a three-dimensional view. The intramembrane morphology, in bi- and three-dimensional views, and immunocytochemical labeling of synaptic contacts with parallel and climbing fibers, basket and stellate cell axons have been characterized. Read More

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April 2012
4 Reads

The actin filament network associated to Sertoli cell ectoplasmic specializations.

Biocell 2011 Dec;35(3):81-9

Instituto de Histología y Embriología "Dr. Mario H. Burgos" (IHEM-CONICET), Facultad de Ciencias Médicas, Universidad Nacional de Cuyo, Mendoza, Argentina.

Junctional devices in Sertoli cells conform the blood-testis barrier and play a key role in maturation and differentiation of germ cells. The spacial distribution of ectoplasmic specializations of Sertoli cells was studied by beta-actin immunolabelling, using laser confocal and transmission electron microscopy. For confocal microscopy, beta-actin immunolabelling of ectoplasmic specializations was studied over the background of either prosaposin or glutaredoxin immunolabelling of the Sertoli cytoplasm. Read More

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December 2011

Apigenin inhibits cell migration through MAPK pathways in human bladder smooth muscle cells.

Biocell 2011 Dec;35(3):71-9

Department of Animal Biology, School of Husbandry and Veterinary Medicine, Jiangsu Polytechnic College of Agriculture and Forestry, Jurong, Jiangsu 212400, China.

Apigenin, a nonmutagenic flavonoid, has been shown to possess free radical scavenging activities, anticarcinogenic properties, antioxidant and anti-inflammatory effects. Recently, apigenin was reported to cause gastric relaxation in murine. To assess possible effects of apigenin on migration of bladder smooth muscle (SM) cell, we isolated SM cells from peri-cancer tissue of human bladder and established a cell model that was capable to overexpress transiently MEKK1 (MEK kinase 1). Read More

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December 2011
2 Reads

Protective effect of aqueous suspension of dried latex of Calotropis procera against oxidative stress and renal damage in diabetic rats.

Biocell 2011 Dec;35(3):63-9

Department of Pharmacology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi - 110 029, India.

Calotropis species have been used in the traditional medicinal system for the treatment of diseases of the liver and abdomen. In view of the antioxidant and anti-hyperglycemic properties of an aqueous suspension obtained from the dried latex of Calotropis procera, the present study was carried out to evaluate its efficacy in affording protection against alloxan induced changes in rat kidney. A single intraperitoneal injection of alloxan (150 mg/kg) in rats produced hyperglycemia within 3 days and altered kidney functions over a period of 90 days. Read More

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December 2011

Assessment of gonadal follicle size in the invading bivalve Limnoperna fortunei (Mytilidae).

Biocell 2011 Aug;35(2):59-62

División Zoología Invertebrados, Museo de La Plata, UNLP, Paseo del Bosque S/N, 1900 La Plata, Argentina.

Limnoperna fortunei is an invasive gonochoristic and byssate freshwater bivalve originary from Southeast Asia. It shows great adaptive-reproductive ability, so knowledge of the gonadal cycle is an important factor for the prevention and control of this bioinvasion. This species is highly damaging to natural and human environments. Read More

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Micrometric measurement of the density of stained odontoblast processes.

Biocell 2011 Aug;35(2):51-7

Histology and Embryology Department, School of Dentistry, National University of Rosario, Argentina.

Unlabelled: The embryological, structural and functional unit of the dentine-pulp complex shares the odontoblast, located in the border of the dentine pulp, with basal nuclei and organelles. The odontoblast process emerges from its apical pole. It is formed by microtubules, microfilaments and vesicles covered by membranes penetrating the dentinal tubules, isolated from the inter-tubular matrix, along the extent of the dentine. Read More

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http://www.cricyt.edu.ar/biocell/vol/pdf/35_2/03.pdf
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Comparison of inflammatory microRNA expression in healthy and periodontitis tissues.

Biocell 2011 Aug;35(2):43-9

Department of Oral Microbiology, School of Dentistry, Pusan National University, Yangsan 626-870, Korea.

MicroRNAs (miRNAs) are short RNA molecules that negatively regulate gene expression primarily by degrading target mRNA or inhibit the translation of protein product. Recently, many reports have shown the altered miRNA expression in various diseases. However, there are no reports on miRNA expression related to periodontitis. Read More

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http://www.cricyt.edu.ar/biocell/vol/pdf/35_2/02.pdf
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August 2011
6 Reads

The ovary of Lagostomus maximus (Mammalia, Rodentia): an analysis by confocal microscopy.

Biocell 2011 Aug;35(2):37-42

CEBBAD - Centro de Estudios Biomédicos Biotecnológicos Ambientales y de Diagnóstico, Universidad Maimónides, Hidalgo 775 - (C 1405BCK), Buenos Aires, Argentina.

Lagostomus maximus is a notable mammalian model for reproductive studies. Females have an extremely high ovulation rate, which is due to down-regulation of the follicular apoptosis pathway, which ensures a large pool of developing follicles. This large pool is supported by the convoluted anatomy of the mature ovary, whose germinal tissue is found in irregularly curved ridges throughout the cortex. Read More

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August 2011
3 Reads
0.571 Impact Factor

A glass bead protocol for recovery of host cell free Ehrlichia canis and quantification by Sybr-green real-time PCR.

Biocell 2011 Apr;35(1):35-6

Unidade de Biotecnologia, UNAERP, Ribeirão Preto, Brazil.

E. canis infection of the canine cell line DH82 is a routine in studies with this bacteria. A protocol for isolation of host cell free bacteria was developed based on the use of glass beads. Read More

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April 2011
4 Reads

Evaluation of potential embryo toxicity of albendazole sulphoxide in CF1 mice.

Biocell 2011 Apr;35(1):29-33

Area de Embriologia, Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires, Tandil, Argentina.

Benzimidazole compounds are used in both humans and animals for controlling helminth parasites. Albendazole has teratogenic effects attributed to its active metabolite albendazole sulphoxide. The aim of this work was to evaluate the effect of the latter compound when administered to pregnant CF1 mice during the preimplantation period. Read More

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April 2011
1 Read

Detection of single copy sequences using BAC-FISH and C-PRINS techniques in sunflower chromosomes.

Biocell 2011 Apr;35(1):19-28

Instituto de Biotecnología, CICVyA, INTA Castelar, N. Repetto y Los Reseros s/n, 1686 Hurlingham, Provincia de Buenos Aires, Argentina.

Bacterial artificial chromosome-fluorescence in situ hybridization (BAC-FISH) and cycling-primed in situ labeling (C-PRINS) techniques were evaluated for integration of physical and genetic maps of sunflower (Helianthus annuus L.). Single-site SSR markers were selected from three linkage groups of a high-density sunflower genetic map. Read More

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Salinity induced anatomical and morphological changes in Chloris gayana Kunth roots.

Biocell 2011 Apr;35(1):9-17

Cáitedra de Morfología Vegetal, Facultad de Ciencias Agrarias, Instituto de Agrobiotecnología del Litoral (CONICET-UNL), Kreder 2805 (S3080HOF), Esperanza, Argentina.

Chloris gayana Kunth is a grass species valuable as forage which was introduced into Argentina to be used as pasture in saline soils of subtropical and warm-temperate zones, given its good adaptability to drought, salinity and mild freezing. However, its tolerance varies according to the cultivar. In tetraploid cultivars, important reductions in yield have been observed. Read More

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April 2011
4 Reads

Bovine parthenogenotes produced by inhibition of first or second polar bodies emission.

Biocell 2011 Apr;35(1):1-7

Laboratorio de Biotecnología Animal, Facultad de Agronomía, Universidad de Buenos Aires. Av. San Martin 4453. C1 417DSE, Argentina.

Parthenogenetic embryos are an ethically acceptable alternative for the derivation of human embryonic stem cells. In this work, we propose a new strategy to produce bovine parthenogenetic embryos inhibiting the emission of the first polar body during in vitro maturation, and allowing the extrusion of the second polar body during oocyte activation. Cytochalasin B, an inhibitor of actin microfilaments, was employed during in vitro maturation to inhibit first polar body emission or during parthenogenetic activation to block second polar body emission. Read More

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Examination of camptothecin and 10-hydroxycamptothecin in Camptotheca acuminata plant and cell culture, and the affected yields under several cell culture treatments.

Biocell 2010 Dec;34(3):139-43

State Key Laboratory of Genetic Engineering, School of Life Sciences, Morgan-Tan International Center for Life Sciences, Fudan-SJTU-Nottingham Plant Biotechnology R&D Center, Fudan University, Shanghai 200433, PR China.

Camptothecin and its derivatives are monoterpenoid indole alkaloids exhibiting significant anti-tumor actions. With the aim of improving the production of these pharmaceuticals, the contents of camptothecin and 10-hydroxycamptothecin in different tissues including roots, stems, leaves, young flower buds, opening flowers, fading flowers and seeds from Camptotheca acuminata, were investigated. The young flower buds had the highest alkaloid concentrations (camptothecin, 2. Read More

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December 2010
9 Reads

Structure of the stigma and style in sunflower (Helianthus annuus L.).

Biocell 2010 Dec;34(3):133-8

Grupo de Biología Reproductiva en Plantas Superiores, Cátedra de Botánica Agrícola, Facultad de Agronomía, Universidad de Buenos Aires, Av. San Martín 4453, (1417) Buenos Aires, Argentina.

This is the first report of the ultrastructure of the stigma and style during and after anthesis in Helianthus annuus L. using light and transmission electron microscopy. The stigma is bifid with unicellular papillae. Read More

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December 2010

WT-1 mRNA expression is modulated by nitric oxide availability and Hsp70 interaction after neonatal unilateral ureteral obstruction.

Biocell 2010 Dec;34(3):121-32

Area de Fisiopatología, Departamento de Patología, Facultad de Ciencias Médicas, Universidad Nacional de Cuyo, Mendoza, Argentina.

Wilms tumor gene 1 (wt-1), a key regulator of mesenchymal-epithelial transformation, is downregulated during congenital obstructive nephropathy, leading to apoptosis. There is a functional interaction between WT-1 and inducible nitric oxide synthase (iNOS). In this regard, we reported that after neonatal unilateral ureteral obstruction, rosuvastatin prevents apoptosis through an increase in nitric oxide bioavailability, which in turn is linked to higher Hsp70 expression. Read More

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December 2010
5 Reads

Influence of estradiol analogue on cell growth, morphology and death in esophageal carcinoma cells.

Biocell 2010 Dec;34(3):113-20

Department of Physiology, University of Pretoria, Pretoria, South Africa.

2-Methoxyestradiol-bis-sulphamate is a bis-sulphamoylated derivative of the naturally occurring 17-beta-estradiol metabolite namely 2-methoxyestradiol. 2-Methoxyestradiol-bis-sulphamate is regarded as a potential anticancer drug with increased antiproliferative activity when compared to 2-methoxyestradiol. The aim of this pilot in vitro study was to determine the influence of 2-methoxyestradiol-bis-sulphamate on cell growth, morphology and possible induction of certain types of cell death in the SNO esophageal carcinoma cell line. Read More

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December 2010

Sperm ultrastructure of Mytella (Bivalvia) populations from distinct habitats along the northern coast of São Paulo State, Brazil.

Biocell 2010 Dec;34(3):103-11

Departamento de Anatomia, Biologia Celular e Fisiologia e Biofísica, Instituto de Biologia, Universidade Estadual de Campinas, CP 6109, 13083-863 Campinas, SP, Brazil.

Ultrastructural analyses of bivalve spermatozoa are relevant in studies that aim to identify taxonomic traits for the purposes of discriminating species and conducting phylogenetic studies. In the present work, spermatozoa of mussel specimens of the genus Mytella, collected from two populations living in distinct habitats, were examined by electron microscopy. The objective was to identify sperm ultrastructural taxonomic traits that could be used to differentiate Mytella species. Read More

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December 2010
1 Read

Antiproliferative and genotoxic effects of Mikania glomerata (Asteraceae).

Biocell 2010 Dec;34(3):95-101

Department of Biology, Universidade Federal de Santa Maria, UFSM, Santa Maria, RS, Brazil.

Mikania glomerata is a plant used in Brazilian traditional medicine, known as 'guaco'. It possesses anti-inflammatory properties and the aqueous extracts of its leaves are indicated for the treatment of diseases of the respiratory tract. This study aimed at evaluating the antiproliferative and genotoxic effect of Mikania glomerata leaf infusions on the cell cycle of onion. Read More

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December 2010
12 Reads

CD44 is involved in CXCL-12 induced acute myeloid leukemia HL-60 cell polarity.

Biocell 2010 Aug;34(2):91-4

Department of Laboratory Medicine, First Affiliated Hospital, China Medical University, NO.155, North Nanjing Street, Heping District, Shenyang 110001, China.

CXCL-12 and its receptor CXCR4 participate in breast cancer and melanoma cell metastasis to bone and lymphoid nodes. CD44, as a receptor for hyaluronic acid, is involved in lymphocyte recirculation, homing, adhesion and migration. But the role of CD44 in CXCL-12 induced leukemia cell migration still remains unclear. Read More

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August 2010
5 Reads

Nuclear pores in luteal cells during pregnancy and after parturition and pup removal in the rat. A freeze-fracture study.

Biocell 2010 Aug;34(2):81-9

Instituto de Histología y Embriología "Dr. Mario H. Burgos" (IHEM), CONICET, Facultad de Ciencias Médicas, Universidad Nacional de Cuyo, Argentina.

In a previous paper we described a pronounced increase of apoptotic nuclei in rat corpus luteum of pregnancy whose programmed chromatin degeneration was induced by the progesterone antagonist mifepristone. Those observations encouraged us to study the apoptotic nuclear membrane during pregnancy and after parturition and pup removal, by using a freeze-fracture technique which allows us to observe 'en face' the nuclear envelop and also permits nuclear pore counting. This study was complemented with the TUNEL assay (TdT-mediated dUTP nick-end labelling). Read More

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In vitro effects of 2-methoxyestradiol-bis-sulphamate on cell growth, morphology and cell cycle dynamics in the MCF-7 breast adenocarcinoma cell line.

Biocell 2010 Aug;34(2):71-9

Department of Physiology, University of Pretoria, P.O. Box 2034, Pretoria 0001, South Africa.

In the search for new and improved anticancer therapies, researchers have identified several potentially useful compounds. One of these agents is 2-methoxyestradiol-bis-sulphamate (2ME-BM), a sulphamoylated derivative of 2-methoxyestradiol. The objective of this study was to evaluate 2ME-BM's in vitro efficacy as antiproliferative agent in the MCF-7 breast adenocarcinoma cell line. Read More

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August 2010
10 Reads

Variable number tandem repeats in the promoter region of prostacyclin synthase gene in choline deficient rats.

Biocell 2010 Aug;34(2):65-70

Centre of Experimental Pathology, Department of Pathology, Faculty of Medicine, University of Buenos Aires (CONICET), Buenos Aires, Argentina.

Weanling Sprague-Dawley rats were fed on a choline-deficient diet with hydrogenated vegetable oil and corn oil as lipids develop acute renal failure. Pathogenesis of the latter is controversial and an ischemic mechanism has been proposed. Arachidonic acid derivatives are involved in the regulation of vascular tonus. Read More

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August 2010
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NFAT regulates CSF-1 gene transcription triggered by L-selectin crosslinking.

Biocell 2010 Aug;34(2):57-63

Centre for Bioengineering and Biotechnology, China University of Petroleum (East China), Qingdao 266555, PR China.

L-selectin is a member of the selectin family that play an important role both in mediating the initial capture and subsequent rolling of leukocytes along the endothelial cells. Furthermore, L-selectin can function as a signal molecule. In our previous studies, we reported that L-selectin ligation could regulate CSF-1 (colony-stimulating factor-1) gene transcription, in which AP-1 acts as a crucial transcriptional factor. Read More

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August 2010
11 Reads