6,009 results match your criteria Bioanalysis [Journal]


Initial evaluation of PET/CT with F-FSU-880 targeting prostate-specific membrane antigen in prostate cancer patients.

Cancer Sci 2018 Dec 13. Epub 2018 Dec 13.

Department of Diagnostic Imaging and Nuclear Medicine, Graduate School of Medicine, Kyoto University, Kyoto, Japan.

This first-in-man study was performed to evaluate the safety, whole-body distribution, dose estimation and lesion accumulation of F-FSU-880, a newly-developed probe targeting prostate-specific membrane antigen (PSMA). Six prostate cancer patients with known metastatic lesions underwent serial whole-body positron emission tomography/computed tomography (PET/CT) with F-FSU-880. Blood and urine were analyzed before and after PET/CT. Read More

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December 2018

Dual-Wavelength Ratiometric Electrochemiluminescence Immunosensor for Cardiac Troponin I Detection.

Anal Chem 2018 Dec 14. Epub 2018 Dec 14.

Ratiometric electrochemiluminescence (ECL) has attracted special focus in biological analysis field because it could eliminate the environmental interference to gain precise measurement. Herein, a dual-wavelength ratiometric ECL biosensor was designed for detection of cardiac troponin I (cTnI), where (4,4'-dicarboxylicacid-2,2'-bipyridyl) ruthenium(II) (Ru(dcbpy)32+) and Au nanoparticles loaded graphene oxide/polyethyleneimine (GPRu-Au) nanomaterial acts as an acceptor, and Au nanoparticles modified graphitic phase carbon nitride nanosheets composite (Au-CNN) acts as donor. Au-CNN shows high and steady ECL signal centered at 455 nm, which is well matched with the adsorption of GPRu-Au, thereby a high efficient electrochemiluminescent resonance energy transfer (ECL-RET) sensing platform is designed. Read More

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December 2018
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Subgingival lipid A profile and endotoxin activity in periodontal health and disease.

Clin Oral Investig 2018 Dec 12. Epub 2018 Dec 12.

Faculty of Medicine and Dentistry, University of Plymouth, Plymouth, PL4 8AA, UK.

Objectives: Regulation of lipopolysaccharide (LPS) chemical composition, particularly its lipid A domain, is an important, naturally occurring mechanism that drives bacteria-host immune system interactions into either a symbiotic or pathogenic relationship. Members of the subgingival oral microbiota can critically modulate host immuno-inflammatory responses by synthesizing different LPS isoforms. The objectives of this study were to analyze subgingival lipid A profiles and endotoxin activities in periodontal health and disease and to evaluate the use of the recombinant factor C assay as a new, lipid A-based biosensor for personalized, point-of-care periodontal therapy. Read More

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December 2018

A novel and safe small molecule enhances hair follicle regeneration by facilitating metabolic reprogramming.

Exp Mol Med 2018 Dec 6;50(12):160. Epub 2018 Dec 6.

Department of Functional Genomics, Korea University of Science & Technology (UST), 217 Gajungro, Yuseong-gu, Daejeon, 34113, Republic of Korea.

Targeting hair follicle regeneration has been investigated for the treatment of hair loss, and fundamental studies investigating stem cells and their niche have been described. However, knowledge of stem cell metabolism and the specific regulation of bioenergetics during the hair regeneration process is currently insufficient. Here, we report the hair regrowth-promoting effect of a newly synthesized novel small molecule, IM176OUT05 (IM), which activates stem cell metabolism. Read More

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December 2018

Voltammetric analysis of dantrolene and its active metabolite with indomethacin in rat plasma.

Bioanalysis 2018 Dec 12. Epub 2018 Dec 12.

Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, University of Alexandria, El-Messalah, Alexandria 21521, Egypt.

Aim: Differential pulse polarography was used for the concurrent analysis of the coadministered dantrolene (DAN) and indomethacin (IND) in plasma.

Materials & Methods: DAN and IND, Hanging mercury drop electrode and Britton-Robinson buffer at pH 5 were used. In plasma, cathodic reduction of DAN nitro group and its active metabolite at -0. Read More

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December 2018

Miniaturized sample preparation methods for saliva analysis.

Bioanalysis 2019 Jan 12;11(2):119-148. Epub 2018 Dec 12.

Department of Chemistry, Faculty of Science, Qom University, Qom, Iran.

Saliva, as the first body fluid encountering with the exogenous materials, has good correlation with blood and plays an important role in bioanalysis. However, saliva has not been studied as much as the other biological fluids mainly due to restricted access to its large volumes. In recent years, there is a growing interest for saliva analysis owing to the emergence of miniaturized sample preparation methods. Read More

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January 2019

The role of roughage provision on the absorption and disposition of the mycotoxin deoxynivalenol and its acetylated derivatives in calves: from field observations to toxicokinetics.

Arch Toxicol 2018 Dec 10. Epub 2018 Dec 10.

Department of Pharmacology, Toxicology and Biochemistry, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.

A clinical case in Belgium demonstrated that feeding a feed concentrate containing considerable levels of deoxynivalenol (DON, 1.13 mg/kg feed) induced severe liver failure in 2- to 3-month-old beef calves. Symptoms disappeared by replacing the highly contaminated corn and by stimulating ruminal development via roughage administration. Read More

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December 2018
1 Read

Metabolomic-Based Methods in Diagnosis and Monitoring Infection Progression.

Exp Suppl 2018 ;109:283-315

Center for Metabolomics and Bioanalysis (CEMBIO), CEU San Pablo University, Boadilla del Monte, Spain.

A robust biomarker screening and validation is crucial for overcoming the current limits in the clinical management of infectious diseases. In this chapter, a general workflow for metabolomics is summarized. Subsequently, an overview of the major contributions of this omics science to the field of biomarkers of infectious diseases is discussed. Read More

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January 2018

Copper in wood preservatives delayed wood decomposition and shifted soil fungal but not bacterial community composition.

Appl Environ Microbiol 2018 Dec 7. Epub 2018 Dec 7.

Institute for Bioanalysis, Department of Applied Sciences, Coburg University of Applied Sciences and Arts, Coburg, Germany

Copper-based fungicides are routinely used for wood and plant protection, which can lead to an enrichment of copper-tolerant microbial communities in soil. To investigate the effect of such wood preservatives on soil fungal and bacterial community composition, five different vineyard and fruit-growing soil environments were evaluated using incubation studies over time. Pine sapwood specimens were impregnated with either water or different biocide treatment solutions containing a mixture of copper, triazoles and quaternary ammonium compounds (CuTriQAC), a mixture of triazoles and quaternary ammonium compounds (TriQAC) or copper alone (Cu). Read More

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December 2018

Oxidative stress markers in saliva and plasma differ between diet-controlled and insulin-controlled gestational diabetes mellitus.

Diabetes Res Clin Pract 2018 Dec 6. Epub 2018 Dec 6.

Department of Bioanalysis and Drugs Analysis, Faculty of Pharmacy, Medical University of Warsaw, 1 Banacha Street, 02-097 Warsaw, Poland.

Objectives: The aims of the study were as follows: to investigate possible differences between plasma oxidative status (OS) in late-onset GDM and well-characterized healthy pregnant women (oral health, diet); to verify the existence of possible differences between GDMG1 (diet-treated) and GDMG2 (insulin-treated GDM); to determine whether oxidative stress markers could be detected in saliva.

Material And Methods: A total of 89 pregnant women (n=89; 59 with GDM and 30 controls) were evaluated. Malondialdehyde (MDA), total antioxidant capacity (ORAC), inactivation of aldehyde dehydrogenase (I), activity of glutathione peroxidase (GPx) and glutathione transferase (GST)) in plasma and/or saliva were analyzed. Read More

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December 2018

Development and validation of bioanalytical liquid chromatography-tandem mass spectrometry method for the estimation of pentoxifylline in human plasma: Application for a comparative pharmacokinetic study.

Eur J Mass Spectrom (Chichester) 2018 Dec 10:1469066718817929. Epub 2018 Dec 10.

4 Biotechnica Pharma, Hyderabad, India.

A method for bioanalysis of pentoxifylline in human plasma was developed using liquid chromatography-tandem mass spectrometry, which is simple, specific, and sensitive. Pentoxifylline D5 was used as the internal standard. Employing only 100 µl of human plasma, processing was done with solid-phase extraction technique. Read More

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December 2018

Juvenile rat and pediatric trazodone studies: how to gain extra sensitivity to overcome bioanalytical challenges.

Bioanalysis 2019 Jan 10;11(1):21-32. Epub 2018 Dec 10.

Angelini RR&D (Research, Regulatory & Development), Angelini SpA, Piazzale della Stazione snc, 00071 - S Palomba-Pomezia (Rome), Italy.

Aim: Trazodone (TZD) is used for the treatment of depression in adults and, off-label, as a sleep medication in adult and pediatric populations. The off-label use is well documented, however further clinical studies are needed to confirm its efficacy and safety for the treatment of sleep disorders. In this scenario, we developed a bioanalytical method to quantify low TZD concentrations in samples collected by capillary microsampling (CMS) to support dose finding, Good Laboratory Practice juvenile rat toxicokinetic and upcoming pediatric studies. Read More

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January 2019
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Detection and prioritization of developmentally neurotoxic and/or neurotoxic compounds using zebrafish.

Toxicol Sci 2018 Dec 5. Epub 2018 Dec 5.

BIOBIDE, Donostia - San Sebastián, Gipuzkoa, Spain.

The standard methods for toxicity testing using rodent models cannot keep pace with the increasing number of chemicals in our environment due to time and resource limitations. Hence, there is an unmet need for fast, sensitive and cost-effective alternate models to reliably predict toxicity. As part of Tox21 Phase III's effort, a 90-compound library was created and made available to researchers to screen for neurotoxicants using novel technology and models. Read More

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December 2018
1 Read

Amazing pleiotropic effects of azithromycin.

Authors:
Lies Lahousse

Breathe (Sheff) 2018 Dec;14(4):336-337

Dept Bioanalysis - Pharmaceutical Care Unit, FFW- Campus Heymans, Ghent, Belgium.


http://ow.ly/WGlz30lLL7z. Read More

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December 2018
1 Read

Generation of a highly efficient and tissue-specific tryptophan hydroxylase 1 knockout mouse model.

Sci Rep 2018 Dec 5;8(1):17642. Epub 2018 Dec 5.

Graduate School of Medical Science and Engineering, Korea Advanced Institute of Science and Technology, Daejeon, 34141, Republic of Korea.

Recent studies on tissue-autonomous serotonin (5-hydroxytryptamine [5-HT]) function have identified new roles for 5-HT in peripheral organs. Most of these studies were performed by crossing mice carrying the Tph1 allele with tissue specific Cre mice. In the present study, we found that 5-HT production was not completely abolished in Tph1 KO mice. Read More

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December 2018

Application of combined solid-state NMR and DFT calculations for the study of piracetam polymorphism.

Solid State Nucl Magn Reson 2018 Nov 22;97:17-24. Epub 2018 Nov 22.

Faculty of Pharmacy with the Laboratory Medicine Division, Medical University of Warsaw, Department of Biomaterials Chemistry, Chair of Analytical Chemistry and Biomaterials, Banacha 1, 02-093 Warsaw, Poland.

Piracetam, a popular nootropic drug, widely used in the treatment of age-associated mental decline and disorders of the nervous system such as Alzheimer's disease and dementia exists under normal pressure in three polymorphic forms (P1, P2 and P3) of different stability. In this work the relative stability of piracetam polymorphs depending on the temperature was studied using the ssNMR spectroscopy combined with ab initio DFT calculations. The ssNMR spectroscopy enabled the analysis of polymorphic phase transition in the case of pure active substance as well as polymorphic form identification in the analysis of the commercial solid dosage formulations. Read More

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November 2018
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Cuvette-based microfluidic device integrated with nanostructures for measuring dual Localized Surface Plasmon Resonance (LSPR) signals.

Rev Sci Instrum 2018 Nov;89(11):113107

University of Science and Technology, 217 Gajeong-ro, Yoseong-gu, Daejeon 34113, South Korea.

A spectrophotometer that uses a localized surface plasmon resonance phenomenon is a powerful measurement tool in the biotechnology and bioanalysis fields. We propose a novel cuvette design type that can be used for universal spectrophotometers. The novel cuvette design needs a few l reagent for measuring, and also two chips for measurement can be loaded and measured at the same time. Read More

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November 2018

Interaction of Arylidenechromanone/Flavanone Derivatives with Biological Macromolecules Studied as Human Serum Albumin Binding, Cytotoxic Effect, Biocompatibility Towards Red Blood Cells.

Molecules 2018 Dec 1;23(12). Epub 2018 Dec 1.

Department of Cosmetic Raw Materials Chemistry, Faculty of Pharmacy, Medical University of Lodz, ulMuszynskiego 1, 90-151 Lodz, Poland.

The aim of this study was to determine the cytotoxic effect of 3-arylidenechromanone (1) and 3arylideneflavanone (2) on HL-60 and NALM-6 cell lines (two human leukemia cell lines) and a WM-115 melanoma cell line. Both compounds exhibited high cytotoxic activity with higher cytotoxicity exerted by compound 2, for which IC values below 10 µM were found for each cell line. For compound 1, the IC values were higher than 10 µM for HL-60 and WM-115 cell lines, but IC < 10 µM was found for the NALM-6 cell line. Read More

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December 2018

Review of DBS methods as a quantitative tool for anticancer drugs.

Biomed Chromatogr 2018 Dec 4:e4445. Epub 2018 Dec 4.

Jubilant Biosys, 2nd Stage, Industrial Suburb, Yeswanthpur, Bangalore, India.

An overview of published dried blood spot (DBS) methods for the quantitation of various classes of anticancer drugs from clinical and preclinical studies is presented. The increased reporting of DBS methods in literature for quantitation of various classes of drugs is a true testimony of its utility in bioanalytical application. While DBS offers several advantages as compared to conventional wet sampling techniques, still there remain a number of nuances that may impede the assay adaptability of DBS method in routine quantitative bioanalysis. Read More

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December 2018
2 Reads

Exploring the Fundamental Structures of Life: Non-targeted, Chemical Analysis of Single Cells and Subcellular Structures.

Angew Chem Int Ed Engl 2018 Nov 30. Epub 2018 Nov 30.

University of Illinois, Department of Chemistry, 600 S. Mathews Ave. 63-5, 61801-5004, Urbana, UNITED STATES.

Cells are a basic functional and structural unit of living organisms. Both unicellular communities and multicellular species produce an astonishing chemical diversity, enabling a wide range of divergent functions, yet each cell shares numerous aspects that are common to all living organisms. While there are many approaches for studying this chemical diversity, only a few are non-targeted and capable of analyzing hundreds of different chemicals at cellular resolution. Read More

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November 2018
1 Read

Antibiotic use in children with asthma: cohort study in UK and Dutch primary care databases.

BMJ Open 2018 Nov 28;8(11):e022979. Epub 2018 Nov 28.

Department of Medical Informatics, Erasmus University, Rotterdam, The Netherlands.

Objectives: To compare the rate, indications and type of antibiotic prescriptions in children with and without asthma.

Design: A retrospective cohort study.

Setting: Two population-based primary care databases: Integrated Primary Care Information database (IPCI; the Netherlands) and The Health Improvement Network (THIN; the UK). Read More

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November 2018
4 Reads

2018 White Paper on Recent Issues in Bioanalysis: focus on immunogenicity assays by hybrid LBA/LCMS and regulatory feedback (Part 2 - PK, PD & ADA assays by hybrid LBA/LCMS & regulatory agencies' inputs on bioanalysis, biomarkers and immunogenicity).

Bioanalysis 2018 Nov 29. Epub 2018 Nov 29.

F Hoffmann-La Roche, Basel, Switzerland.

The 2018 12 Workshop on Recent Issues in Bioanalysis took place in Philadelphia, PA, USA on April 9-13, 2018 with an attendance of over 900 representatives from pharmaceutical/biopharmaceutical companies, biotechnology companies, contract research organizations and regulatory agencies worldwide. WRIB was once again a 5-day, week-long event - a full immersion week of bioanalysis, biomarkers and immunogenicity. As usual, it was specifically designed to facilitate sharing, reviewing, discussing and agreeing on approaches to address the most current issues of interest including both small- and large-molecule bioanalysis involving LCMS, hybrid LBA/LCMS and LBA/cell-based assays approaches. Read More

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November 2018

2018 White Paper on Recent Issues in Bioanalysis: focus on flow cytometry, gene therapy, cut points and key clarifications on BAV (Part 3 - LBA/cell-based assays: immunogenicity, biomarkers and PK assays).

Bioanalysis 2018 Nov 29. Epub 2018 Nov 29.

Amador Bioscience, Pleasanton, CA, USA (formerly of OncoMed, Redwood City, CA, USA).

The 2018 12 Workshop on Recent Issues in Bioanalysis took place in Philadelphia, PA, USA on April 9-13, 2018 with an attendance of over 900 representatives from pharmaceutical/biopharmaceutical companies, biotechnology companies, contract research organizations and regulatory agencies worldwide. WRIB was once again a 5-day full immersion in bioanalysis, biomarkers and immunogenicity. As usual, it was specifically designed to facilitate sharing, reviewing, discussing and agreeing on approaches to address the most current issues of interest including both small- and large-molecule bioanalysis involving LCMS, hybrid LBA/LCMS and LBA/cell-based assays approaches. Read More

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November 2018

2018 White Paper on Recent Issues in Bioanalysis: 'A global bioanalytical community perspective on last decade of incurred samples reanalysis (ISR)' (Part 1 - small molecule regulated bioanalysis, small molecule biomarkers, peptides & oligonucleotide bioanalysis).

Bioanalysis 2018 Nov 29;10(22):1781-1801. Epub 2018 Nov 29.

UK MHRA, London, UK.

The 2018 12 Workshop on Recent Issues in Bioanalysis (12th WRIB) took place in Philadelphia, PA, USA on April 9-13, 2018 with an attendance of over 900 representatives from pharmaceutical/biopharmaceutical companies, biotechnology companies, contract research organizations and regulatory agencies worldwide. WRIB was once again a 5-day full immersion in bioanalysis, biomarkers and immunogenicity. As usual, it was specifically designed to facilitate sharing, reviewing, discussing and agreeing on approaches to address the most current issues of interest including both small- and large-molecule bioanalysis involving LC-MS, hybrid ligand binding assay (LBA)/LC-MS and LBA/cell-based assays approaches. Read More

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November 2018
2 Reads

Tutorial: Volumetric absorptive microsampling (VAMS).

Anal Chim Acta 2019 Jan 5;1046:32-47. Epub 2018 Sep 5.

Pharmaco-Toxicological Analysis Laboratory (PTA Lab), Department of Pharmacy and Biotechnology (FaBiT), Alma Mater Studiorum - University of Bologna, Bologna, Italy. Electronic address:

Volumetric absorptive microsampling (VAMS) is a recent microsampling technique used to obtain dried specimens of blood and other biological matrices for application to a plethora of bioanalytical purposes. As such, it can be likened to dried blood spot (DBS) technique that has been in wide use for the last 40 years. However, VAMS promises to bring some significant advantages over DBS, related to sampling volume accuracy, haematocrit (HCT) dependence, pre-treatment and automation. Read More

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January 2019

In-and-Out Molecular Changes Linked to the Type 2 Diabetes Remission after Bariatric Surgery: An Influence of Gut Microbes on Mitochondria Metabolism.

Int J Mol Sci 2018 Nov 24;19(12). Epub 2018 Nov 24.

Clinical Research Centre, Medical University of Bialystok, 15-276 Bialystok, Poland.

Different kinds of gastrointestinal tract modulations known as "bariatric surgery" are actually the most effective treatment for obesity and associated co-morbidities, such as type 2 diabetes (T2DM). The potential causes of those effects have yet to be explained. In our study, we focused on molecular changes evoked by laparoscopic sleeve gastrectomy leading to T2DM remission. Read More

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November 2018
1 Read

Fungal Endophytes Control and Reduce Trichothecenes and Zearalenone in Maize.

Toxins (Basel) 2018 Nov 24;10(12). Epub 2018 Nov 24.

Laboratory of Applied Mycology and Phenomics, Department of Plants and Crops, Faculty Bioscience Engineering, Ghent University, 9000 Ghent, Belgium.

can cause Giberella Ear Rot (GER) and seedling blight in maize, resulting in major yield losses. Besides GER, the infected grains are consequently contaminated with multiple mycotoxins of . Zearalenone and trichothecenes, such as deoxynivalenol and its acetylated forms, are among the major mycotoxins associated with infection in maize. Read More

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November 2018
6 Reads

Metformin induces significant reduction of body weight, total cholesterol and LDL levels in the elderly - A meta-analysis.

PLoS One 2018 26;13(11):e0207947. Epub 2018 Nov 26.

Institute for Translational Medicine, Medical School, University of Pécs, Pécs, Hungary.

Background: Metformin is the first-choice drug for patients with Type 2 diabetes, and this therapy is characterized by being weight neutral. However, in the elderly an additional unintentional weight loss could be considered as an adverse effect of the treatment.

Objectives: We aimed to perform a meta-analysis of placebo-controlled studies investigating the body weight changes upon metformin treatment in participants older than 60 years. Read More

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November 2018

Identifying Single Viruses Using Biorecognition Solid-State Nanopores.

J Am Chem Soc 2018 Nov 26. Epub 2018 Nov 26.

The Institute of Scientific and Industrial Research , Osaka University , 8-1 Mihogaoka , Ibaraki, Osaka 567-0047 , Japan.

Immunosensing is a bioanalytical technique capable of selective detections of pathogens by utilizing highly specific and strong intermolecular interactions between recognition probes and antigens. Here, we exploited the molecular mechanism in artificial nanopores for selective single-virus identifications. We designed hemagglutinin antibody mimicking oligopeptides with a weak affinity to influenza A virus. Read More

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November 2018
2 Reads

Transdermal sampling of vitamin D and 25-hydroxyvitamin D.

Bioanalysis 2018 Nov 26. Epub 2018 Nov 26.

Department of Pharmaceutical Sciences, Albany College of Pharmacy & Health Sciences, 106 New Scotland Avenue, Albany, NY 12208, USA.

Aim: Transdermal analysis is proposed for vitamin D and its hydroxylated metabolite to overcome problems associated with blood analysis.

Methods: Vitamin D was extracted directly from skin with solid patches and liquid phases. Deuterium-labeled vitamin D was added to the extraction solutions to compensate for variability and accurately determine the rate of transdermal transfer. Read More

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November 2018

Analytical techniques for characterization of biological molecules - proteins and aptamers/oligonucleotides.

Bioanalysis 2018 Nov 26. Epub 2018 Nov 26.

Discipline of Biosciences & Biomedical Engineering, Indian Institute of Technology Indore, Indore 453552, India.

With the advent of the high-throughput technologies and exciting times for biology, the discipline of analytical methodology is experiencing a surge in the growth and the scope. Over the years, multitude of analytical techniques have evolved from a work-intensive, low sensitivity and high volume of reagent and sample consumption endeavor to automated, better selectivity, lower limit of quantification and cost-effective techniques for biological research. In this review, we give an overview of the currently available wide range of cell-based and noncell based and structural based analytical techniques, their principle and biological applications. Read More

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November 2018

On-line monitoring human breath acetone during exercise and diet by proton transfer reaction mass spectrometry.

Bioanalysis 2018 Nov 26. Epub 2018 Nov 26.

Anhui Province Key Laboratory of Medical Physics & Technology, Center of Medical Physics & Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui, PR China.

Aim: This study aims to develop a method for monitoring fat loss by detection of breath acetone.

Methods: A new method combining direct breath sampling system and proton transfer reaction MS (PTR-MS) was developed for on-line detection of breath acetone. The breath acetone of 272 volunteers was detected respectively when exercise or diet. Read More

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November 2018
2 Reads

European Bioanalysis Forum recommendation on the best practices to demonstrate processed sample stability.

Bioanalysis 2019 Jan 26;11(1):7-11. Epub 2018 Nov 26.

European Bioanalysis Forum, Havenlaan 86c B204, 1000 Brussels, Belgium.

The European Bioanalysis Forum is providing a proposal on experiments to be conducted if processed samples are to be stored for a period of time, either after an initial injection or if processed samples are to be stored for a period of time prior to their first injection. Using survey information and extensive discussion an insight was gained as to whether the current practices employed during method validation were conducted to scientifically validate actual sample handling conditions or to simply meet a perceived regulatory expectation. The goal of this report is to recommend the adoption of scientifically justified validation experiments that exactly reproduce and processed test sample handling and storage procedures and to introduce some uniformity across the industry. Read More

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January 2019
5 Reads

A novel HPLC-DAD method for simultaneous determination of febuxostat and diclofenac in biological samples: pharmacokinetic outcomes.

Bioanalysis 2019 Jan 26;11(1):41-54. Epub 2018 Nov 26.

Department of Pharmacology & Toxicology, Faculty of Pharmacy, Alexandria University, El-Messalah, Alexandria 21521, Egypt.

Aim: To develop a simple HPLC-DAD method for simultaneous determination of febuxostat (FEB) and diclofenac (DIC) in biological samples to assess pharmacokinetic outcomes of their coadministration. Methodology & results: Sample preparation was performed by liquid-liquid extraction. Drugs analysis was done on C18 column using methanol-formic acid pH 2. Read More

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January 2019

The importance of evaluating the chemical structures and strategies to avoid pitfalls in quantitative bioanalysis.

Bioanalysis 2018 Nov 26. Epub 2018 Nov 26.

Bioanalysis, Immunogenicity, & Biomarkers, PTS-In Vivo/In Vitro Translation, GlaxoSmithKline Pharmaceuticals, 709 Swedeland Road, King of Prussia, PA 19406, USA.

Quantitative bioanalytical data are crucial in pharmaceutical research and development, allowing project teams to make informed scientific decisions on the progression of candidate molecules to medicines. Many challenges are often encountered during the bioanalysis of drugs in biological matrices which require resolution in a timely manner. In this publication, guidance is provided to bioanalytical scientists on how to identify potential problems before they become an obstacle for the drug development and to share our experiences dealing some of most common problems encountered in the bioanalytical laboratory. Read More

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November 2018
1 Read

Selol (Se IV) modulates adhesive molecules in control and TNF-α-stimulated HMEC-1 cells.

J Trace Elem Med Biol 2019 Jan 5;51:106-114. Epub 2018 Oct 5.

Department of Biochemistry, Medical University of Warsaw, 02-097 Warszawa, Banacha 1, Poland. Electronic address:

Selol, an organic selenitetrigliceride formulation containing selenium at +4 oxidation level, has been suggested as anticancer drug. One of the causes of several diseases including cancer may be inflammation. This study aimed at determining the activity of Selol via measuring its effect on reactive oxygen species (ROS) generation, nuclear factor kappa B (NF-κB) activation, intercellular cell adhesion molecules-1 (ICAM-1), vascular cell adhesive molecule-1 (VCAM-1), and plateled-endothelial cell adhesive molecule-1 (PECAM-1) levels on control and on tumor necrosis factor-α (TNF-α)-stimulated human microvascular endothelial cells (HMEC-1). Read More

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January 2019
8 Reads

Biotherapeutic Antibody Subunit LC-MS and Peptide Mapping LC-MS Measurements to Study Possible Biotransformation and Critical Quality Attributes In Vivo.

J Pharm Sci 2018 Nov 19. Epub 2018 Nov 19.

Bioanalysis, Immunogenicity and Biomarkers, In Vitro/In Vivo Translation Platform.

Biotransformation monitoring involves tracking drug modification occurring during in-life studies. Critical Quality Attribute (CQA) monitoring from forced degraded drug material or in-life sample sets can provide an in-depth assessment of product quality for support in early or late stage drug development. For CQA analysis, biotherapeutic mAb subunit analysis and peptide mapping liquid chromatography-mass spectrometry (LC-MS) approaches are utilized, although typically from an in vitro setting (e. Read More

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November 2018
1 Read

Application of Mitra microsampling for pharmacokinetic bioanalysis of monoclonal antibodies in rats.

Bioanalysis 2019 Jan 21;11(1):13-20. Epub 2018 Nov 21.

Biotherapeutics Discovery, Boehringer Ingelheim Pharmaceuticals, Inc., 900 Ridgebury Road, Ridgefield CT 06877, USA.

Aim: Volumetric absorptive microsampling (VAM) is being increasingly applied in nonclinical pharmacokinetic studies. Although there are published results for VAM use in small molecule pharmacokinetics (PK) studies, there is limited data on the utility of VAM for protein therapeutics.

Results: We describe the use of Mitra microsampler for blood sampling, ELISA quantitation and PK analysis of two marketed therapeutic monoclonal antibodies administered to rat. Read More

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January 2019

A novel approach to the application of hexagonal mesoporous silica in solid-phase extraction of drugs.

Heliyon 2018 Nov 12;4(11):e00930. Epub 2018 Nov 12.

Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.

Mesoporous silica with hexagonal type structure containing amine functional group was introduced. Firstly, aminopropyl hexagonal mesoporous silica was synthetized in a co-condensation process, via templating route of -dodecylamine. Then synthesized mesoporous material were characterized, and FT-IR spectrum confirmed the presence of amine group and CHN analysis determined the amount of organic layer. Read More

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November 2018
2 Reads

Non-separative mass spectrometry methods for non-invasive medical diagnostics based on volatile organic compounds: A review.

Anal Chim Acta 2019 Jan 5;1045:10-22. Epub 2018 Jul 5.

Departamento de Química Analítica, Nutrición y Bromatología Facultad de Ciencias Químicas, Universidad de Salamanca, 37008 Salamanca, Spain.

In this review, an assessment of non-separative methods based on mass spectrometry used to analyse volatile organic compounds in the field of bioanalysis is performed. The use of non-separative methods based on mass spectrometry has been established as an attractive option for analysing compounds. These instrumental configurations are suitable for biomedical applications because of their versatility, rapid output of results, and the wide range of volatile organic compounds that can be determined. Read More

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January 2019
5 Reads

Comparative study of the chemiluminescence of coelenterazine, coelenterazine-e and Cypridina luciferin with an experimental and theoretical approach.

J Photochem Photobiol B 2018 Nov 13;190:21-31. Epub 2018 Nov 13.

Chemistry Research Unit (CIQUP), Department of Chemistry and Biochemistry, Faculty of Sciences of University of Porto, R. Campo Alegre 687, 4169-007 Porto, Portugal; LACOMEPHI, GreenUP, Department of Geosciences, Environment and Territorial Planning, Faculty of Sciences of University of Porto, R. Campo Alegre 687, 4169-007 Porto, Portugal. Electronic address:

Imidazopyrazinone is a typical scaffold present in marine bioluminescence, in which thermal energy is converted into excitation energy in an enzyme-catalyzed reaction. In fact, the imidazopyrazinone scaffold is a common link among organisms of eight phyla. The characterization of the light emission mechanism is essential for the development of future applications in bioimaging, bioanalysis and biomedicine. Read More

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November 2018
5 Reads

Knock-downs for drought-induced cysteine-protease genes alter barley leaf structure and the response to abiotic and biotic stresses.

J Exp Bot 2018 Nov 18. Epub 2018 Nov 18.

Centro de Biotecnología y Genómica de Plantas (CBGP, UPM-INIA). Universidad Politécnica de Madrid (UPM) - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA). Campus de Montegancedo, Pozuelo de Alarcón, Madrid, Spain.

To survive under water deprivation, plants alter gene expression patterns, make structural and physiological adjustments and optimize the efficient use of water. Protein turn-over is required for a rapid degradation of proteins and an effective nutrient recycling. In barley, the transcriptional responses of the C1A cysteine protease family to drought revealed the up-regulation of four genes in stressed leaves. Read More

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November 2018
3 Reads

Validation and clinical application of a novel LC-MS method for quantification of dolutegravir in breast milk.

Bioanalysis 2018 Nov 19. Epub 2018 Nov 19.

Department of Molecular & Clinical Pharmacology, University of Liverpool, 70 Pembroke Place, Liverpool, L69 3GF, UK.

Aim: A novel, sensitive and reproducible method for quantification of dolutegravir (DTG) in dried breast milk spots (DBMS) was developed and validated for use in clinical studies. Its application enabled measurement of DTG pharmacokinetics in breastfeeding mothers and their infants. Results/methodology: Sample extraction was by liquid-liquid extraction using Tert-butyl methy-ether, with DTG-d5 as an internal standard. Read More

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November 2018
3 Reads

Acceleration of nano-surface and molecular-orientation limited (nSMOL) proteolysis with acidified reduction pretreatment for quantification of Tocilizumab.

J Pharm Biomed Anal 2018 Nov 8;164:467-474. Epub 2018 Nov 8.

Leading Technology of Bioanalysis and Protein Chemistry, SHIMADZU Corporation, Japan; SHIMADZU Bioscience Research Partnership, Shimadzu Scientific Instruments, United States. Electronic address:

Antibody drugs are effective therapeutic agents and provide treatment for many types of diseases such as cancer and rheumatoid arthritis. Because many antibody drugs are developed and approved, quantification technologies of antibodies are required for drug development and individualized therapy. We recently reported a high reproducible and robust therapeutic drug monitoring method for antibody drugs. Read More

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November 2018
3 Reads

Serotonin signals through a gut-liver axis to regulate hepatic steatosis.

Nat Commun 2018 11 16;9(1):4824. Epub 2018 Nov 16.

Graduate School of Medical Science and Engineering, KAIST, Daejeon, 34141, Republic of Korea.

Nonalcoholic fatty liver disease (NAFLD) is increasing in worldwide prevalence, closely tracking the obesity epidemic, but specific pharmaceutical treatments for NAFLD are lacking. Defining the key molecular pathways underlying the pathogenesis of NAFLD is essential for developing new drugs. Here we demonstrate that inhibition of gut-derived serotonin synthesis ameliorates hepatic steatosis through a reduction in liver serotonin receptor 2A (HTR2A) signaling. Read More

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November 2018
4 Reads

Ultrasensitive Photoelectrochemical Assay with PTB7-Th/CdTe Quantum Dots Sensitized Structure as Signal Tag and Benzo-4-chlorohexadienone Precipitate as Efficient Quencher.

Anal Chem 2018 Nov 29. Epub 2018 Nov 29.

Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering , Southwest University , Chongqing 400715 , People's Republic of China.

Herein, an ultrasensitive photoelectrochemical (PEC) assay was developed for the monitoring of microRNA-141 (miRNA-141) based on poly{4,8-bis[5-(2-ethylhexyl)thiophen-2-yl]benzo[1,2- b:4,5- b']dithiophene-2,6-diyl- alt-3-fluoro-2-[(2-ethylhexyl)carbonyl]thieno[3,4- b]thiophene-4,6-diyl}/CdTe quantum dots ( PTB7-Th/CdTe QDs) sensitized structure as signal tag and the benzo-4-chlorohexadienone (4-CD) precipitate as efficient quencher. PTB7-Th and CdTe QDs were successively modified on the electrode surface to form a novel sensitized structure with eminent photovoltaic performances and surpassing film-forming ability. The PTB7-Th/CdTe QDs sensitized structure served as signal tag for achieving a strong initial PEC signal. Read More

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November 2018
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Resistive pulse sensing as particle counting and sizing method in microfluidic systems: Designs and applications review.

J Sep Sci 2018 Nov 16. Epub 2018 Nov 16.

Department of Bioanalytical Instrumentation, Institute of Analytical Chemistry of the CAS, Brno, Czech Republic.

Resistive pulse sensing is a well-known and established method for counting and sizing particles in ionic solutions. Throughout its development the technique has been expanded from detection of biological cells to counting nanoparticles and viruses, and even registering individual molecules, e.g. Read More

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November 2018
1 Read

Advances in Optical Sensing and Bioanalysis Enabled by 3D Printing.

ACS Sens 2018 Nov 30. Epub 2018 Nov 30.

Department of Chemistry , University of California , Riverside , California 92521 , United States.

The recent explosion of 3D printing applications in scientific literature has expanded the speed and effectiveness of analytical technological development. 3D printing allows for manufacture that is simply designed in software and printed in-house with nearly no constraints on geometry, and analytical methodologies can thus be prototyped and optimized with little difficulty. The versatility of methods and materials available allows the analytical chemist or biologist to fine-tune both the structural and functional portions of their apparatus. Read More

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November 2018

Development of paper substrate for paper spray MS in high-sensitivity analysis of biological samples.

Bioanalysis 2018 Nov 16. Epub 2018 Nov 16.

College of Chemistry & Chemical Engineering, Xi'an Shiyou University, Xi'an 710065, PR China.

Paper spray (PS) has demonstrated a promising future for direct mass spectrometric analysis. In the process of PS, paper substrate has been demonstrated as a crucial factor in determining the final performance of PS-MS, and therefore much attention is paid to modification of paper substrate. In this review, we systematically introduce the development of paper substrate for PS. Read More

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November 2018