- James R Krycer,
- Ciana Diskin,
- Marin E Nelson,
- Xiao-Yi Zeng,
- Daniel J Fazakerley,
- David E James
Biotechniques 2018 Jan 1;64(1):27-29. Epub 2018 Jan 1.
School of Life and Environmental Sciences, Charles Perkins Centre, The University of Sydney.
Research into cellular metabolism has become more high-throughput, with typical cell-culture experiments being performed in multiwell plates (microplates). This format presents a challenge when trying to collect gaseous products, such as carbon dioxide (CO2), which requires a sealed environment and a vessel separate from the biological sample. To address this limitation, we developed a gas trapping protocol using perforated plastic lids in sealed cell-culture multiwell plates. Read More