6,721 results match your criteria BioTechniques[Journal]


Idiosyncrasies of thermofluorimetric aptamer binding assays.

Biotechniques 2019 Feb 15. Epub 2019 Feb 15.

University of Idaho, Dept of Chemistry, 875 Perimeter Dr, Moscow, ID 83844-2343, USA.

To explore thermofluorimetric analysis (TFA) in detail, we compared two related aptamers. The first, LINN2, is a DNA aptamer previously selected against EGFR recombinant protein. In this work we selected a second aptamer, KM4, against EGFR-overexpressing A549 cells. Read More

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http://dx.doi.org/10.2144/btn-2018-0128DOI Listing
February 2019

Improved locus-specific unmethylated controls for MS-HRM analysis derived from 5-aza-2-deoxycytidine-treated DNA.

Biotechniques 2019 Feb 14. Epub 2019 Feb 14.

University of Maribor, Faculty of Chemistry & Chemical Engineering, Smetanova ulica 17, 2000 Maribor, Slovenia.

We report two restriction enzyme-based approaches for generating clean locus-specific unmethylated controls for methylation-sensitive high-resolution melting (MS-HRM) analyses. These unmethylated standards are derived from DNA treated with the demethylating agent 5-aza-2-deoxycytidine (5-Aza-dc). By using them, we overcome a limitation of 5-Aza-dc treatment - incomplete demethylation at various genomic regions. Read More

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http://dx.doi.org/10.2144/btn-2018-0161DOI Listing
February 2019

From Sanger sequencing to genome databases and beyond.

Biotechniques 2019 Feb;66(2):60-63

We look at how next-generation sequencing has advanced research across different disease fields, and the growing importance of open access genomic databases. Read More

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http://dx.doi.org/10.2144/btn-2019-0011DOI Listing
February 2019

A high-throughput protocol for isolating cell-free circulating tumor DNA from peripheral blood.

Biotechniques 2019 Feb;66(2):85-92

Canada's Michael Smith Genome Sciences Centre, BC Cancer, 675 West 10th Avenue, Vancouver, BC, Canada.

The analysis of cell-free circulating tumor DNA (ctDNA) is potentially a less invasive, more dynamic assessment of cancer progression and treatment response than characterizing solid tumor biopsies. Standard isolation methods require separation of plasma by centrifugation, a time-consuming step that complicates automation. To address these limitations, we present an automatable magnetic bead-based ctDNA isolation method that eliminates centrifugation to purify ctDNA directly from peripheral blood (PB). Read More

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https://www.future-science.com/doi/10.2144/btn-2018-0148
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http://dx.doi.org/10.2144/btn-2018-0148DOI Listing
February 2019
1 Read

Improved protocol for isolation of high-quality total RNA from different organs of Phaseolus vulgaris L.

Biotechniques 2019 Feb;66(2):96-98

Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México (UNAM), Av. Universidad 2001, Cuernavaca, 62210 México.

A modified protocol was developed to obtain high-quality total RNA from various mature organs, including leaves, seeds, pods and testae, from different cultivars of Phaseolus vulgaris L. grown under optimal conditions or subjected to severe drought; stress conditions leading to the accumulation of numerous secondary metabolites can affect RNA quality. This modified procedure is based on CTAB extraction protocols. Read More

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http://dx.doi.org/10.2144/btn-2018-0129DOI Listing
February 2019

A method for visualizing fluorescence of flavonoid therapeutics in vivo in the model eukaryote Dictyostelium discoideum.

Biotechniques 2019 Feb;66(2):65-71

School of Science, University of Greenwich, Chatham Maritime, ME4 4TB, UK.

Naturstoff reagent A (diphenylboric acid 2-aminoethyl ester [DPBA]) has been used historically in plant science to observe polyphenolic pigments, such as flavonoids, whose fluorescence requires enhancement to be visible by microscopy. Flavonoids are common dietary constituents and are the focus of considerable attention because of their potential as novel therapies for numerous diseases. The molecular basis of therapeutic activity is only gradually being established, and one strand of such research is making use of the social amoeba Dictyostelium discoideum. Read More

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http://dx.doi.org/10.2144/btn-2018-0084DOI Listing
February 2019

New tech for personalized medicine.

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Biotechniques 2019 Feb;66(2):59

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http://dx.doi.org/10.2144/btn-2019-0007DOI Listing
February 2019

Rapid quantification of cellular proliferation and migration using ImageJ.

Biotechniques 2019 Feb;66(2):99-102

Discipline of Biochemistry, School of Life Sciences, University of KwaZulu-Natal, Private Bag X01, Scottsville 3209, South Africa.

Cellular proliferation and migration are crucial during development, regeneration and disease. Methods to quantify these processes are available; however, many are time consuming and require specialized equipment and costly reagents. Simple cell counts (proliferation analysis) and the scratch assay (migration analysis) are favorable methods due to their simplicity and cost-effectiveness; however, they rely on subjective and labor-intensive manual analysis, resulting in low throughput. Read More

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http://dx.doi.org/10.2144/btn-2018-0132DOI Listing
February 2019

Single nucleotide recognition using a probes-on-carrier DNA chip.

Biotechniques 2019 Feb;66(2):73-78

Area of Bioscience & Biotechnology, School of Materials Science, Japan Advanced Institute of Science & Technology (JAIST), 1-1 Asahidai, Nomi, Ishikawa 923-1292, Japan.

Following the sequencing of the human genome, SNP analysis of individual patients has become essential for achieving the best drug response and ensuring optimal care. In this study, we developed a cost-effective probes-on-carrier DNA chip for the detection of SNPs. Our chips harbored three different probes against the TP53 gene, and were capable of detecting wild-type TP53 and SNPs such as rs121912651 and rs11540652. Read More

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http://dx.doi.org/10.2144/btn-2018-0088DOI Listing
February 2019

A simple dialysis device for large DNA molecules.

Biotechniques 2019 Feb;66(2):93-95

Laboratory for Molecular & Computational Genomics, UW Biotechnology Center, University of Wisconsin-Madison, USA.

The potential of genomic DNA is realized when new modalities are invented that manipulate large DNAs with minimal breakage or loss of sample. Here, we describe a polydimethylsiloxane-polycarbonate membrane device to remove small molecules from a sample while retaining large DNAs. Dialysis rates dramatically change as DNA size in kb (M) increases and DNA dimensions become comparable to pore size, and chain characteristics go from rod-like to Gaussian. Read More

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http://dx.doi.org/10.2144/btn-2018-0133DOI Listing
February 2019

Decontamination of Gilson® Pipettes Using High Intensity UV LED.

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Biotechniques 2019 Feb;66(2):103

Having clean pipettes is a consistent need in any laboratory. Current methods of cleaning, however, are not without downfalls. The process is often time-consuming and utilizes expensive chemicals that can leave residue and eventually cause corrosion. Read More

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http://dx.doi.org/10.2144/btn-2018-2015APDOI Listing
February 2019

Recent advances in photo-crosslinkable hydrogels for biomedical applications.

Biotechniques 2019 Jan;66(1):40-53

Faculty of Medicine, University of Dundee, Dow Street, Dundee DD1 5EH, UK.

Photo-crosslinkable hydrogels have recently attracted significant scientific interest. Their properties can be manipulated in a spatiotemporal manner through exposure to light to achieve the desirable functionality for various biomedical applications. This review article discusses the recent advances of the most common photo-crosslinkable hydrogels, including poly(ethylene glycol) diacrylate, gelatin methacryloyl and methacrylated hyaluronic acid, for various biomedical applications. Read More

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http://dx.doi.org/10.2144/btn-2018-0083DOI Listing
January 2019
1 Read

Combining CRISPR/Cas9-mediated knockout with genetic complementation for in-depth mechanistic studies in human ES cells.

Biotechniques 2019 Jan;66(1):23-27

Department of Genetics and Yale Stem Cell Center, Yale University, New Haven, CT, USA.

Gene regulatory networks that control pluripotency of human embryonic stem cells (hESCs) are of considerable interest for regenerative medicine. RNAi and CRISPR/Cas9 technologies have allowed the identification of hESC regulators on a genome-wide scale. However, these technologies are ill-suited for mechanistic studies because knockdown/knockout clones of essential genes do not grow in culture. Read More

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https://www.future-science.com/doi/10.2144/btn-2018-0115
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January 2019
4 Reads

How to transition from 'early career' to 'established'.

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Biotechniques 2019 Jan;66(1):9-11

PhDs, postdocs, fellows and newly appointed lecturers all fall into the category of early-career researchers (ECRs). The life of researchers in early stages of their career is far from straightforward. Funding is a huge barrier for many ECRs looking to make the next step in their career, but it's not the only challenge. Read More

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http://dx.doi.org/10.2144/btn-2018-0182DOI Listing
January 2019

The unsustainable lab.

Authors:
Abigail Sawyer

Biotechniques 2019 Jan;66(1):5-7

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http://dx.doi.org/10.2144/btn-2018-0185DOI Listing
January 2019

Bead-based assay for spatiotemporal gene expression control in cell-free transcription-translation systems.

Biotechniques 2019 Jan;66(1):29-33

Universität des Saarlandes, Biologische Experimentalphysik, Naturwissenschaftlich-Technische Fakultät, B2 1, Campus, 66123 Saarbrücken, Germany.

Cell-free gene expression has applications in synthetic biology, biotechnology and biomedicine. In this technique gene expression regulation plays an important role. Transcription factors do not completely suppress expression while other methods for expression control, for example CRISPR/Cas, often require important biochemical modifications. Read More

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http://dx.doi.org/10.2144/btn-2018-0097DOI Listing
January 2019

A convenient method to generate and maintain poly(A)-encoding DNA sequences required for in vitro transcription of mRNA.

Biotechniques 2019 Jan;66(1):37-39

Wits/SAMRC Antiviral Gene Therapy Research Unit, School of Pathology, Faculty of Health Sciences, University of the Witwatersrand, Private Bag 3, WITS 2050, Johannesburg, South Africa.

Generating mRNA in vitro to encode therapeutic or cell-modifying proteins is rapidly gaining favor. An important factor that determines efficiency of translation from in vitro transcribed mRNA is the length of the 3' poly(A) sequence. However, reproducibly generating and maintaining templates from circular plasmids to have consistent lengths of the homo poly(A) sequences is challenging. Read More

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http://dx.doi.org/10.2144/btn-2018-0120DOI Listing
January 2019

Assessment of cell yield among different devices for endovascular biopsy to harvest vascular endothelial cells.

Biotechniques 2019 Jan;66(1):34-36

Department of Radiology & Biomedical Imaging, Division of Neurointerventional Radiology, University of California - San Francisco, San Francisco, CA, USA.

Endovascular biopsy can increase understanding of vascular disease by granting access to epigenetic data that are not normally attainable. This study compares biopsy yields among multiple devices used, examining differences in cell counts according to species, device type, sampling location and disease state. Chi-square analysis compared means of cells harvested with respect to these variables. Read More

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http://dx.doi.org/10.2144/btn-2018-0099DOI Listing
January 2019

A look back at 2018.

Biotechniques 2019 Jan;66(1):12-13

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http://dx.doi.org/10.2144/btn-2018-0181DOI Listing
January 2019

Automated RNA Purification directly from TRIzol® on the Hamilton Microlab® STAR™.

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Biotechniques 2018 Nov;65(5):293-294

The TRIzol method for RNA extraction has been the gold standard. The powerful protein denaturant effectively stabilizes RNA and inactivates RNases and infectious agents. However, the need for phase separation, precipitation, and potential phenol carryover can further complicate workflows. Read More

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http://dx.doi.org/10.2144/btn-2018-2010APDOI Listing
November 2018

Quantitative clonal barcode analysis for the identification of key drivers of disease progression and drug resistance.

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Biotechniques 2018 Sep;65(3):169

The ability to label and trace individual cells is a powerful experimental approach in many research areas, including stem cell biology, evolution and cancer biology. Labeling of individual cancer cells with different engineered or natural driver lesions and modeling of in vivo or in vitro clonal expansion can facilitate discovery of genetic determinants of increased pathogenicity, cancer progression, or drug resistance. Read More

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http://dx.doi.org/10.2144/btn-2018-2002APDOI Listing
September 2018

Benefits of Synchronising LED Illumination Systems to a Camera.

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Biotechniques 2018 Dec;65(6):364

To enable simple and effective high titer recombinant lentivirus production, we examined key parameters for the generation of lentivirus including: transfection conditions, DNA vector selection, media change, and incubation time. Adherent or suspension adapted 293-derived cells can be used as production hosts. These results illustrate the importance of optimizing transfection processes for high titer recombinant lentivirus production. Read More

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http://dx.doi.org/10.2144/btn-2018-2012DOI Listing
December 2018
1 Read

Electroporation Optimization: A User's Guide.

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Biotechniques 2018 Dec;65(6):361-363

Electroporation is an effective physical method for delivery of various types and sizes of molecules to cells in culture. It is a fast and relatively easy way to re-engineer cells to contain exogeneous nucleic acids or small molecules. Electroporation conditions, including strength of the pulse and buffer composition, can have profound effects on the efficiency of delivery and cellular viability post-electroporation, thereby influencing the total number of cells available for experimentation. Read More

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http://dx.doi.org/10.2144/btn-2018-2011DOI Listing
December 2018

Use of the Precellys® Evolution homogenizer for unbiased DNA extraction from the ZymoBIOMICS™ microbial community standard.

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Biotechniques 2018 Jul;65(1):47-48

Microbiome profiling via Next Generation Sequencing (NGS) techniques is rapidly changing the landscape of microbiology. However, to ensure accurate microbiome measurements, it is essential to ensure the microbiome workflow is unbiased and robust [1]. For instance, it has been reported that bias in microbiomics analyses can be introduced at every step [2-4] including the DNA extraction step if microbial cells are not uniformly and completely lysed. Read More

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http://dx.doi.org/10.2144/btn-2017-2000DOI Listing

Sample Collection / Preservation and DNA Isolation from stool samples for MICROBIOME analysi.

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Biotechniques 2019 Jan;66(1):54

Appropriate preservation and storage of stool samples is crucial to maintain DNA integrity and microbial community composition for downstream applications and analysis, including NGS and microbiome characterization. Read More

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http://dx.doi.org/10.2144/btn-2018-2014DOI Listing
January 2019

Preparing low-copy number plasmid DNA using the ZymoPURE II Plasmid Maxiprep Kit.

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Biotechniques 2018 Dec;65(6):365-367

Plasmids differ significantly in copy number due to their origin of replication, size, and associated insert. Several plasmids contain mutations that allow them to reach very high-copy numbers within the bacterial cell. However, depending on the objectives of the experiment or the nature of the cloned insert, it is not always possible to use high-copy number plasmid DNA. Read More

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http://dx.doi.org/10.2144/btn-2018-2013DOI Listing
December 2018

Homogenization of 6 mouse brains in a 15ml Precellys® tube using the Precellys® Evolution.

Authors:

Biotechniques 2018 Oct;65(4):231

The laboratory of Bertin Pharma works in the field of pharmacy, biotechnologies, cosmetics and nutrition (GLP & GMP). It is specialized in bioanalysis, formulation analysis, pesticide assays, and membrane protein quantification. Read More

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http://dx.doi.org/10.2144/btn-2018-2007DOI Listing
October 2018

Development and Optimization of a High Titer Recombinant Lentivirus System.

Authors:

Biotechniques 2018 Oct;65(4):232-234

To enable simple and effective high titer recombinant lentivirus production, we examined key parameters for the generation of lentivirus including: transfection conditions, DNA vector selection, media change, and incubation time. Adherent or suspension adapted 293-derived cells can be used as production hosts. These results illustrate the importance of optimizing transfection processes for high titer recombinant lentivirus production. Read More

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http://dx.doi.org/10.2144/btn-2018-2008DOI Listing
October 2018
1 Read

New year, new BioTechniques.

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Biotechniques 2019 Jan;66(1)

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https://www.future-science.com/doi/10.2144/btn-2018-0186
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http://dx.doi.org/10.2144/btn-2018-0186DOI Listing
January 2019
2 Reads

A method for rapid analysis of the root hydrotropic response in Arabidopsis thaliana.

Biotechniques 2019 Jan 11. Epub 2019 Jan 11.

Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Av. Universidad 2001, Col. Chamilpa, Cuernavaca, Mor., 62210, México.

The system for analyzing the hydrotropic curvature with a moisture gradient in wild-type Arabidopsis roots was modified. Optimal conditions were determined for detecting a hydrotropic curvature of 90° just after 4 h of stimulation. This system only requires 15 ml of a solution of KCO with a density of 1. Read More

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https://www.future-science.com/doi/10.2144/btn-2018-0173
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http://dx.doi.org/10.2144/btn-2018-0173DOI Listing
January 2019
4 Reads

Development of a motion-based cell-counting system for Trypanosoma parasite using a pattern recognition approach.

Biotechniques 2018 Dec 13. Epub 2018 Dec 13.

Artificial Intelligence Research Center, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan.

Automated cell counters that utilize still images of sample cells are widely used. However, they are not well suited to counting slender, aggregate-prone microorganisms such as Trypanosoma cruzi. Here, we developed a motion-based cell-counting system, using an image-recognition method based on a cubic higher-order local auto-correlation feature. Read More

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http://dx.doi.org/10.2144/btn-2018-0163DOI Listing
December 2018
1 Read

Oxford nanopore sequencing enables rapid discovery of single-domain antibodies from phage display libraries.

Biotechniques 2018 Dec;65(6):351-356

Human Health Therapeutics Research Centre, National Research Council Canada, 100 Sussex Drive, Ottawa, Ontario, Canada, K1A 0R6.

Antibody (Ab) repertoire sequencing using high-throughput massively parallel technologies has contributed substantially to the understanding of Ab responses following infection, vaccination and autoimmunity. Because individual B-cell receptors are recombined and diversified somatically, genomic comparisons are limited, and distinguishing rare variants from sequencing errors is a major challenge. Oxford Nanopore Technologies' MinION is a highly portable and cost-effective third-generation sequencing instrument, but has not been used for Ab repertoire sequencing due to its high error rate (approximately 1/10 bases). Read More

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http://dx.doi.org/10.2144/btn-2018-0123DOI Listing
December 2018
2 Reads

Rapid selection of single-stranded DNA aptamers binding Staphylococcus epidermidis in platelet concentrates.

Biotechniques 2018 Dec;65(6):331-338

Division of Emerging & Transfusion-Transmitted Diseases, Laboratory of Bacterial & Transmissible Spongiform Encephalopathy Agents, Center for Biologics Evaluation & Research, Office of Blood Research & Review, US Food & Drug Administration, 10903 New Hampshire Avenue, Silver Spring, MD 20993, USA.

Staphylococcus epidermidis is the most common transfusion-associated pathogen contaminating platelet concentrates. Methods to reduce or eliminate contaminating bacteria from platelet units are critical for improving the safety of blood transfusions. We used rapid isolation of DNA aptamers (RIDA) to identify single-stranded (ss)DNA aptamers as ligands that specifically bind to S. Read More

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http://dx.doi.org/10.2144/btn-2018-0081DOI Listing
December 2018
2 Reads

Samasy: an automated system for sample selection and robotic transfer.

Biotechniques 2018 Dec;65(6):357-360

Department of Epidemiology & Biostatistics, University of California, San Francisco, CA, USA.

Sample automation and management is increasingly important as the number and size of population-scale and high-throughput projects grow. This is particularly the case in large-scale population studies where sample size is far outpacing the commonly used 96-well plate format. To facilitate management and transfer of samples in this format, we present Samasy, a web-based application for the construction of a sample database, intuitive display of sample and batch information, and facilitation of automated sample transfer or subset. Read More

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http://dx.doi.org/10.2144/btn-2018-0090DOI Listing
December 2018
2 Reads

DNA barcodes from snake venom: a broadly applicable method for extraction of DNA from snake venoms.

Biotechniques 2018 Dec;65(6):339-345

School of Biological Sciences, University of Northern Colorado, 501 20th Street, Greeley, CO 80639-0017, USA.

DNA barcoding is a simple technique used to develop a large-scale system of classification that is broadly applicable across a wide variety of taxa. DNA-based analysis of snake venoms can provide a system of classification independent of currently accepted taxonomic relationships by generating DNA barcodes specific to each venom sample. DNA purification from dried snake venoms has previously required large amounts of starting material, has resulted in low yields and inconsistent amplification, and was possible with front-fanged snakes only. Read More

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http://dx.doi.org/10.2144/btn-2018-0096DOI Listing
December 2018
1 Read

Updates from the Society for Neuroscience 2018.

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Biotechniques 2018 Dec;65(6):303

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http://dx.doi.org/10.2144/btn-2018-0170DOI Listing
December 2018
1 Read

QuickCount: a novel automated software for rapid cell detection and quantification.

Biotechniques 2018 Dec;65(6):322-330

Head & Neck Cancer Research Team, Cancer Research Malaysia, Subang Jaya, Selangor, Malaysia.

We describe a novel automated cell detection and counting software, QuickCount (QC), designed for rapid quantification of cells. The Bland-Altman plot and intraclass correlation coefficient (ICC) analyses demonstrated strong agreement between cell counts from QC to manual counts (mean and SD: -3.3 ± 4. Read More

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https://www.future-science.com/doi/10.2144/btn-2018-0072
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http://dx.doi.org/10.2144/btn-2018-0072DOI Listing
December 2018
19 Reads
2.948 Impact Factor

Tech news: stem cells for modeling and curing disease.

Authors:
Freya Leask

Biotechniques 2018 Dec;65(6):305-310

Freya Leask explores developments in cell culture and stem cell research that are revolutionizing how diseases are studied and treated. Read More

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http://dx.doi.org/10.2144/btn-2018-0171DOI Listing
December 2018
1 Read

3D-printed magnetic tweezers for dorsal traction force measurement.

Biotechniques 2018 Dec;65(6):347-349

Department of Mechanical Engineering, Johns Hopkins University, MD, USA.

In this study, economic magnetic tweezers (EMT) with a sharp gradient field were designed and built, in order to facilitate accurate force measurement. Our design costs less than 40 USD and is easy to mount onto most microscope stages. We leverage the computational fluidic dynamics techniques to calculate the forces based on the results obtained using our simple device. Read More

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http://dx.doi.org/10.2144/btn-2018-0078DOI Listing
December 2018
1 Read

Advances in cancer modeling: fluidic systems for increasing representativeness of large 3D multicellular spheroids.

Biotechniques 2018 Dec;65(6):312-314

Advanced Research Centre on Electronic Systems for Information & Communication Technologies 'E. De Castro' (ARCES), University of Bologna, Italy.

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http://dx.doi.org/10.2144/btn-2018-0153DOI Listing
December 2018
1 Read

Can a new microscopy platform help to improve clinical outcomes?

Biotechniques 2018 Nov;65(5):250-251

Department of Physics, Virginia Commonwealth University, Richmond, VA, USA.

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https://www.future-science.com/doi/10.2144/btn-2018-0102
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November 2018
14 Reads

Chromium sequencing: the doors open for genomics of obligate plant pathogens.

Biotechniques 2018 Nov;65(5):253-257

Department of Biochemistry, Genetics & Microbiology, Tree Protection Co-operative Programme (TPCP), Forestry & Agricultural Biotechnology Institute (FABI), University of Pretoria, South Africa.

It is challenging to sequence and assemble genomes of obligate plant pathogens and microorganisms because of limited amounts of DNA, comparatively large genomes and high numbers of repeat regions. We sequenced the 1.2 gigabase genome of an obligate rust fungus, Austropuccinia psidii, the cause of rust on Myrtaceae, with a Chromium 10X library. Read More

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https://www.future-science.com/doi/10.2144/btn-2018-0019
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http://dx.doi.org/10.2144/btn-2018-0019DOI Listing
November 2018
8 Reads

CRISPR editing validation, immunostaining and DNA sequencing of individual fixed bovine embryos.

Biotechniques 2018 Nov;65(5):281-283

Department of Animal Science, University of California - Davis, Davis, CA, USA.

CRISPR technologies used for mammalian embryology have wide implications from basic research to applications in agriculture and biomedicine. Confirmation of successful gene editing following CRISPR/Cas9 delivery is often limited to either protein expression or sequencing analyses of embryos but not both, due to technical challenges. Herein we report an integrative approach for evaluating both protein expression and genotype of single embryos from fixed bovine embryos previously subjected to CRISPR/Cas9 microinjection. Read More

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https://www.future-science.com/doi/10.2144/btn-2018-0051
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http://dx.doi.org/10.2144/btn-2018-0051DOI Listing
November 2018
11 Reads

Software to improve transfer and reproducibility of cell culture methods.

Biotechniques 2018 Nov;65(5):289-292

CultureTrax®, Cellara LLC, Madison, WI 53719, USA.

Cell culture is a vital component of laboratories throughout the scientific community, yet the absence of standardized protocols and documentation practice challenges laboratory efficiency and scientific reproducibility. We examined the effectiveness of a cloud-based software application, CultureTrax as a tool for standardizing and transferring a complex cell culture protocol. The software workflow and template were used to electronically format a cardiomyocyte differentiation protocol and share a digitally executable copy with a different lab user. Read More

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https://www.future-science.com/doi/10.2144/btn-2018-0062
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http://dx.doi.org/10.2144/btn-2018-0062DOI Listing
November 2018
10 Reads

When can we believe what we see?

Biotechniques 2018 Nov;65(5):245-249

Unitec House, 2 Albert Place, London, N3 1QB, UK.

Abigail Sawyer and Joseph Martin investigate how microscopy is evolving in order for cells and processes to be better visualized in their native environments. Read More

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https://www.future-science.com/doi/10.2144/btn-2018-0151
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http://dx.doi.org/10.2144/btn-2018-0151DOI Listing
November 2018
9 Reads

Capture and storage of plant genomic DNA on a readily available cellulose matrix.

Biotechniques 2018 Nov;65(5):285-287

Department of Molecular, Cellular & Biomedical Sciences, University of New Hampshire, 46 College Road, Durham, NH 03824, USA.

Whatman FTA Cards are a fast and efficient method for capturing and storing nucleic acids but can be cost-prohibitive for large numbers of samples. We developed a method that substitutes a readily-available cellulose matrix and homemade washing buffer for commercial FTA Cards and FTA Purification Reagent. This method is suitable for long-term storage of DNA from many plant species prior to PCR. Read More

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https://www.future-science.com/doi/10.2144/btn-2018-0109
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http://dx.doi.org/10.2144/btn-2018-0109DOI Listing
November 2018
10 Reads

Development of a universal internal positive control.

Authors:
Mark F Kavlick

Biotechniques 2018 Nov;65(5):275-280

Counterterrorism & Forensic Science Research Unit, Laboratory Division, Federal Bureau of Investigation, 2501 Investigation Parkway, Quantico, VA 22135, USA.

Described here is a novel, universal exogenous internal positive control (IPC), which is fully synthetic for unparalleled quality control. The IPC was rationally designed, is small and efficiently amplified, has been successfully utilized alone or in triplex qPCR reactions, and is not crossreactive to human DNA or to any of the numerous non-human DNA samples tested. It was sensitive to inhibition by at least four commonly encountered amplification inhibitors. Read More

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https://www.future-science.com/doi/10.2144/btn-2018-0034
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http://dx.doi.org/10.2144/btn-2018-0034DOI Listing
November 2018
7 Reads

Congratulations to this year's Nobel Prize winners!

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Biotechniques 2018 Nov;65(5):243

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http://dx.doi.org/10.2144/btn-2018-0155DOI Listing
November 2018
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Cassette hybridization for vector assembly application in antibody chain shuffling.

Biotechniques 2018 Nov;65(5):269-274

Institute for Research in Molecular Medicine, Universiti Sains Malaysia, 11800 Penang, Malaysia.

Gene assembly methods are an integral part of molecular cloning experiments. The majority of existing vector assembly methods stipulate a need for exonucleases, endonucleases and/or the use of single-stranded DNA as starting materials. Here, we introduced a vector assembly method that employs conventional PCR to amplify stable double-stranded DNA fragments and assembles them into functional vectors specifically for antibody chain shuffling. Read More

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https://www.future-science.com/doi/10.2144/btn-2018-0031
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http://dx.doi.org/10.2144/btn-2018-0031DOI Listing
November 2018
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Clarification and confocal imaging of the nonhuman primate placental micro-anatomy.

Biotechniques 2019 Feb 29;66(2):79-84. Epub 2018 Oct 29.

Department of Obstetrics & Gynecology, Oregon Health & Science University, Portland, OR, USA.

Geometry of the placental villous vasculature is a key determinant of maternal-fetal nutrient exchange for optimal fetal growth. Recent advances in tissue clarification techniques allow for deep high-resolution imaging with confocal microscopy; however, the methodology lacks a signal:noise ratio of sufficient magnitude to allow for quantitative analysis. Thus, we sought to develop a reproducible method to investigate the 3D vasculature of the nonhuman primate placenta for subsequent data analysis. Read More

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http://dx.doi.org/10.2144/btn-2018-0110DOI Listing
February 2019
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