6,729 results match your criteria BioTechniques[Journal]


eeFit: a Microsoft Excel-embedded program for interactive analysis and fitting of experimental dose-response data.

Authors:
Michel Vivaudou

Biotechniques 2019 Apr;66(4):186-193

Institut de Biologie Structurale (IBS), University of Grenoble Alpes, CEA, CNRS, IBS, 38000 Grenoble, France.

We present here a software program dedicated to the fitting of experimental dose-response data, which integrates seamlessly with Excel and allows curve fitting plots and results to reside alongside data within Excel spreadsheets. The program, named eeFit, for Excel-Embedded Fitting software, requires no advanced knowledge of Excel or non-linear least-squares fitting. Any experimental data present in an Excel file, such as dose-effect data obtained with membrane receptor or ion channel ligands, can be graphed and fitted interactively with standard Hill models for activation or inhibition, or with more complex models for biphasic effects resulting from combinations of activation and inhibition. Read More

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https://www.future-science.com/doi/10.2144/btn-2018-0136
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http://dx.doi.org/10.2144/btn-2018-0136DOI Listing
April 2019
6 Reads

Removal of extra sequences with I-SceI in combination with CRISPR/Cas9 technique for precise gene editing in Drosophila.

Biotechniques 2019 Apr;66(4):198-201

Group of Molecular Organization of Genome, Russian Academy of Sciences, 34/5 Vavilov St, Moscow 119334, Russia.

The CRISPR/Cas9 system has recently emerged as a powerful tool for functional genomic studies and has been adopted for many organisms, including Drosophila. Previously, an efficient two-step strategy was developed to engineer the fly genome by combining CRISPR/Cas9 with recombinase-mediated cassette exchange (RMCE). This strategy allows the introduction of designed mutations into a gene of interest in vivo. Read More

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http://dx.doi.org/10.2144/btn-2018-0147DOI Listing

A robust fractionation method for protein subcellular localization studies in Escherichia coli.

Biotechniques 2019 Apr;66(4):171-178

UCB Celltech, Slough, UK.

Fractionation in Gram-negative bacteria is used to identify the subcellular localization of proteins, in particular the localization of exported recombinant proteins. The process of cell fractionation can be fraught with cross-contamination issues and often lacks supporting data for fraction purity. Here, we compare three periplasm extraction and two cell disruption techniques in different combinations to investigate which process gives uncontaminated compartments from Escherichia coli. Read More

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http://dx.doi.org/10.2144/btn-2018-0135DOI Listing
April 2019
1 Read

Tech news.

Biotechniques 2019 Apr;66(4):167-170

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http://dx.doi.org/10.2144/btn-2019-0030DOI Listing

Opening up to open access.

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Biotechniques 2019 Apr;66(4):107

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http://dx.doi.org/10.2144/btn-2019-0028DOI Listing

Immunochromatographic detection of MPB64 secreted from active BCG by heating: toward same-day diagnosis of tuberculosis.

Biotechniques 2019 Apr 8. Epub 2019 Apr 8.

Waseda Research Institute for Science & Engineering, Waseda University, 3-4-1 Okubo, Shinjuku, Tokyo 169-8555, Japan.

We propose a new detection method for a tuberculosis-specific protein, MPB64, obtained from active bacillus Calmette-Guérin (BCG) by heating. When BCG was included in solution at a concentration >2.75 × 10 CFU/ml, our method for collecting MPB64 through heating active BCG combined with an immunochromatographic assay detected active bacilli within 2. Read More

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https://www.future-science.com/doi/10.2144/btn-2019-0020
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http://dx.doi.org/10.2144/btn-2019-0020DOI Listing
April 2019
7 Reads

Rapid identification of genome-edited mesenchymal stem cell colonies via Cas9.

Biotechniques 2019 Mar 29. Epub 2019 Mar 29.

Department of Immunology, School of Medicine, Shenzhen University, Shenzhen, P.R. China.

Mesenchymal stem cells (MSCs) have been intensively investigated and widely applied in regenerative medicine and immune modulation. However, their efficacy declines during the aging or disease process. Thus, genome-edited MSCs with over-expression or inhibition of specific genes hold a great deal of promise in terms of their therapeutic application. Read More

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http://dx.doi.org/10.2144/btn-2018-0183DOI Listing
March 2019
1 Read

Optimizing leading edge F-actin labeling using multiple actin probes, fixation methods and imaging modalities.

Biotechniques 2019 Mar;66(3):113-119

Department of Anatomy & Structural Biology, Albert Einstein College of Medicine, 1300 Morris Park Ave, Bronx, NY 10461, USA.

We systematically evaluated the performance and reliability of several widely used, commercially available actin-filament probes in a highly motile breast adenocarcinoma cell line to optimize the visualization of F-actin-rich dynamic lamellipodia. We evaluated four Phalloidin-fluorophores, two anti-actin antibodies, and three live-cell actin probes in five fixation conditions across three imaging platforms as a basis for the design of optimized protocols. Of the fluorescent phalloidin-dye conjugates tested, Alexa Fluor-488 Phalloidin ranked best in overall labeling of the actin cytoskeleton and maintenance of the fluorescence signal over time. Read More

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http://dx.doi.org/10.2144/btn-2018-0112DOI Listing
March 2019
3 Reads

Liquid array diagnostics: a novel method for rapid detection of microbial communities in single-tube multiplex reactions.

Biotechniques 2019 Mar;66(3):143-149

Department of Chemistry, Biotechnology & Food Sciences, Norwegian University of Life Sciences, P.O. Box 5003, 1432 Aas, Norway.

We present a novel liquid array diagnostics (LAD) method, which enables rapid and inexpensive detection of microbial markers in a single-tube multiplex reaction. We evaluated LAD both on pure cultures, and on infant gut microbiota for a 15-plex reaction. LAD showed more than 80% accuracy of classification and a detection limit lower than 2% of the Illumina reads per sample. Read More

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http://dx.doi.org/10.2144/btn-2018-0134DOI Listing
March 2019
1 Read

Unlabeled image analysis-based cell viability assay with intracellular movement monitoring.

Biotechniques 2019 Mar;66(3):128-133

Biomedical R&D Department, R&D Division, Medical Business Group, Sony Imaging Products & Solutions Inc., Tokyo Medical and Dental University, 1-5-45 Yushima Bunkyo-ku, Tokyo, 113-8510 Japan.

The need for technologies to monitor cell health is increasing with advancements in the field of cell therapy and regenerative medicine. In this study, we demonstrated unlabeled optical metabolic imaging of cultured living cells. This imaging technique is based on motion vector analysis with a block-matching algorithm to compare sequential time-lapse images. Read More

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http://dx.doi.org/10.2144/btn-2018-0157DOI Listing

Electrochemical methods for probing DNA damage mechanisms and designing cisplatin-based combination chemotherapy.

Biotechniques 2019 Mar;66(3):135-142

Department of Chemistry & Waterloo Institute for Nanotechnology, University of Waterloo, 200 University Avenue West, Waterloo, ON N2L 3G1, Canada.

An electrochemical approach was devised for detecting DNA damage and differentiating two DNA damage mechanisms, which is important to the design of new chemotherapeutics. This approach combined two platforms, based on the detection of base damage and DNA strand cleavage. In this work, our approach was demonstrated for the detection of cisplatin-induced DNA damage and the enhancement effects of two electron donors, N,N,N',N'-tetramethyl-p-phenylenediamine (TMPD) and reduced graphene oxide (rGO). Read More

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http://dx.doi.org/10.2144/btn-2018-0106DOI Listing

Grow your own brain.

Authors:
Jenny Straiton

Biotechniques 2019 Mar;66(3):108-112

Jenny Straiton explores the miniature world of organoids and discusses how these small models are making big changes in the world of neurological research. Read More

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http://dx.doi.org/10.2144/btn-2019-0019DOI Listing
March 2019
1 Read

An inexpensive air stream temperature controller and its use to facilitate temperature-controlled behavior in Drosophila.

Biotechniques 2019 Mar;66(3):159-161

University of Virginia, Department of Biology, Charlottesville, VA, USA.

Controlling the environment of an organism has many biologically relevant applications. Temperature-dependent inducible biological reagents have proven invaluable for elucidating signaling cascades and dissection of neural circuits. Here we develop a simple and affordable system for rapidly changing temperature in a chamber housing adult Drosophila melanogaster. Read More

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http://dx.doi.org/10.2144/btn-2018-0152DOI Listing

From the Editor - March 2019.

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Biotechniques 2019 Mar;66(3):107

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http://dx.doi.org/10.2144/btn-2019-0017DOI Listing
March 2019
2 Reads

Rapid extraction of DNA suitable for NGS workflows from bacterial cultures using the PDQeX.

Biotechniques 2019 Feb 28. Epub 2019 Feb 28.

ZyGEM NZ Ltd, Waikato Innovation Park, Ruakura Road, Hamilton 3216, New Zealand.

Background: PDQeX is a novel, single-step DNA extraction method that purifies nucleic acid from sample in under 30 min.

Materials & Methods: Six bacterial suspensions from species with different cell morphologies and growth optima were made. DNA from half the suspension was purified using PDQeX and the other half using a conventional column purification method. Read More

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http://dx.doi.org/10.2144/btn-2019-0006DOI Listing
February 2019
1 Read

Single-step colony assay with autoinduction of scFv expression for the screening of antibody libraries.

Biotechniques 2019 Apr 20;66(4):194-197. Epub 2019 Feb 20.

Structural Physiology Research Group, Biomedical Research Institute, National Institute of Advanced Industrial Science & Technology (AIST), 1-1-1 Higashi, Tsukuba 305-8566 Japan.

We present a simple colony assay method for screening antibody libraries based on autoinduction of antibody fragment expression. This protocol eliminates the need for colony size monitoring and a separate induction step for single-chain Fv (scFv) antibody fragment expression. Here, scFvs are expressed in an automatic and timely fashion during the assay, resulting in high yields of positive clones and substantial time savings. Read More

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http://dx.doi.org/10.2144/btn-2018-0195DOI Listing

Idiosyncrasies of thermofluorimetric aptamer binding assays.

Biotechniques 2019 Feb 15. Epub 2019 Feb 15.

University of Idaho, Dept of Chemistry, 875 Perimeter Dr, Moscow, ID 83844-2343, USA.

To explore thermofluorimetric analysis (TFA) in detail, we compared two related aptamers. The first, LINN2, is a DNA aptamer previously selected against EGFR recombinant protein. In this work we selected a second aptamer, KM4, against EGFR-overexpressing A549 cells. Read More

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http://dx.doi.org/10.2144/btn-2018-0128DOI Listing
February 2019
2 Reads

Improved locus-specific unmethylated controls for MS-HRM analysis derived from 5-aza-2-deoxycytidine-treated DNA.

Biotechniques 2019 Feb 14. Epub 2019 Feb 14.

University of Maribor, Faculty of Chemistry & Chemical Engineering, Smetanova ulica 17, 2000 Maribor, Slovenia.

We report two restriction enzyme-based approaches for generating clean locus-specific unmethylated controls for methylation-sensitive high-resolution melting (MS-HRM) analyses. These unmethylated standards are derived from DNA treated with the demethylating agent 5-aza-2-deoxycytidine (5-Aza-dc). By using them, we overcome a limitation of 5-Aza-dc treatment - incomplete demethylation at various genomic regions. Read More

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http://dx.doi.org/10.2144/btn-2018-0161DOI Listing
February 2019
1 Read

From Sanger sequencing to genome databases and beyond.

Biotechniques 2019 Feb;66(2):60-63

We look at how next-generation sequencing has advanced research across different disease fields, and the growing importance of open access genomic databases. Read More

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http://dx.doi.org/10.2144/btn-2019-0011DOI Listing
February 2019

A high-throughput protocol for isolating cell-free circulating tumor DNA from peripheral blood.

Biotechniques 2019 Feb;66(2):85-92

Canada's Michael Smith Genome Sciences Centre, BC Cancer, 675 West 10th Avenue, Vancouver, BC, Canada.

The analysis of cell-free circulating tumor DNA (ctDNA) is potentially a less invasive, more dynamic assessment of cancer progression and treatment response than characterizing solid tumor biopsies. Standard isolation methods require separation of plasma by centrifugation, a time-consuming step that complicates automation. To address these limitations, we present an automatable magnetic bead-based ctDNA isolation method that eliminates centrifugation to purify ctDNA directly from peripheral blood (PB). Read More

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https://www.future-science.com/doi/10.2144/btn-2018-0148
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http://dx.doi.org/10.2144/btn-2018-0148DOI Listing
February 2019
9 Reads

Improved protocol for isolation of high-quality total RNA from different organs of Phaseolus vulgaris L.

Biotechniques 2019 Feb;66(2):96-98

Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México (UNAM), Av. Universidad 2001, Cuernavaca, 62210 México.

A modified protocol was developed to obtain high-quality total RNA from various mature organs, including leaves, seeds, pods and testae, from different cultivars of Phaseolus vulgaris L. grown under optimal conditions or subjected to severe drought; stress conditions leading to the accumulation of numerous secondary metabolites can affect RNA quality. This modified procedure is based on CTAB extraction protocols. Read More

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http://dx.doi.org/10.2144/btn-2018-0129DOI Listing
February 2019

A method for visualizing fluorescence of flavonoid therapeutics in vivo in the model eukaryote Dictyostelium discoideum.

Biotechniques 2019 Feb;66(2):65-71

School of Science, University of Greenwich, Chatham Maritime, ME4 4TB, UK.

Naturstoff reagent A (diphenylboric acid 2-aminoethyl ester [DPBA]) has been used historically in plant science to observe polyphenolic pigments, such as flavonoids, whose fluorescence requires enhancement to be visible by microscopy. Flavonoids are common dietary constituents and are the focus of considerable attention because of their potential as novel therapies for numerous diseases. The molecular basis of therapeutic activity is only gradually being established, and one strand of such research is making use of the social amoeba Dictyostelium discoideum. Read More

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http://dx.doi.org/10.2144/btn-2018-0084DOI Listing
February 2019
1 Read

New tech for personalized medicine.

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Biotechniques 2019 Feb;66(2):59

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http://dx.doi.org/10.2144/btn-2019-0007DOI Listing
February 2019
2 Reads

Rapid quantification of cellular proliferation and migration using ImageJ.

Biotechniques 2019 Feb;66(2):99-102

Discipline of Biochemistry, School of Life Sciences, University of KwaZulu-Natal, Private Bag X01, Scottsville 3209, South Africa.

Cellular proliferation and migration are crucial during development, regeneration and disease. Methods to quantify these processes are available; however, many are time consuming and require specialized equipment and costly reagents. Simple cell counts (proliferation analysis) and the scratch assay (migration analysis) are favorable methods due to their simplicity and cost-effectiveness; however, they rely on subjective and labor-intensive manual analysis, resulting in low throughput. Read More

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http://dx.doi.org/10.2144/btn-2018-0132DOI Listing
February 2019
1 Read

Single nucleotide recognition using a probes-on-carrier DNA chip.

Biotechniques 2019 02;66(2):73-78

Area of Bioscience & Biotechnology, School of Materials Science, Japan Advanced Institute of Science & Technology (JAIST), 1-1 Asahidai, Nomi, Ishikawa 923-1292, Japan.

Following the sequencing of the human genome, SNP analysis of individual patients has become essential for achieving the best drug response and ensuring optimal care. In this study, we developed a cost-effective probes-on-carrier DNA chip for the detection of SNPs. Our chips harbored three different probes against the TP53 gene, and were capable of detecting wild-type TP53 and SNPs such as rs121912651 and rs11540652. Read More

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http://dx.doi.org/10.2144/btn-2018-0088DOI Listing
February 2019
2 Reads
2.948 Impact Factor

A simple dialysis device for large DNA molecules.

Biotechniques 2019 Feb;66(2):93-95

Laboratory for Molecular & Computational Genomics, UW Biotechnology Center, University of Wisconsin-Madison, USA.

The potential of genomic DNA is realized when new modalities are invented that manipulate large DNAs with minimal breakage or loss of sample. Here, we describe a polydimethylsiloxane-polycarbonate membrane device to remove small molecules from a sample while retaining large DNAs. Dialysis rates dramatically change as DNA size in kb (M) increases and DNA dimensions become comparable to pore size, and chain characteristics go from rod-like to Gaussian. Read More

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http://dx.doi.org/10.2144/btn-2018-0133DOI Listing
February 2019

Decontamination of Gilson® Pipettes Using High Intensity UV LED.

Authors:

Biotechniques 2019 Feb;66(2):103

Having clean pipettes is a consistent need in any laboratory. Current methods of cleaning, however, are not without downfalls. The process is often time-consuming and utilizes expensive chemicals that can leave residue and eventually cause corrosion. Read More

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http://dx.doi.org/10.2144/btn-2018-2015APDOI Listing
February 2019
1 Read

Recent advances in photo-crosslinkable hydrogels for biomedical applications.

Biotechniques 2019 Jan;66(1):40-53

Faculty of Medicine, University of Dundee, Dow Street, Dundee DD1 5EH, UK.

Photo-crosslinkable hydrogels have recently attracted significant scientific interest. Their properties can be manipulated in a spatiotemporal manner through exposure to light to achieve the desirable functionality for various biomedical applications. This review article discusses the recent advances of the most common photo-crosslinkable hydrogels, including poly(ethylene glycol) diacrylate, gelatin methacryloyl and methacrylated hyaluronic acid, for various biomedical applications. Read More

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http://dx.doi.org/10.2144/btn-2018-0083DOI Listing
January 2019
3 Reads

Combining CRISPR/Cas9-mediated knockout with genetic complementation for in-depth mechanistic studies in human ES cells.

Biotechniques 2019 Jan;66(1):23-27

Department of Genetics and Yale Stem Cell Center, Yale University, New Haven, CT, USA.

Gene regulatory networks that control pluripotency of human embryonic stem cells (hESCs) are of considerable interest for regenerative medicine. RNAi and CRISPR/Cas9 technologies have allowed the identification of hESC regulators on a genome-wide scale. However, these technologies are ill-suited for mechanistic studies because knockdown/knockout clones of essential genes do not grow in culture. Read More

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https://www.future-science.com/doi/10.2144/btn-2018-0115
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http://dx.doi.org/10.2144/btn-2018-0115DOI Listing
January 2019
15 Reads

How to transition from 'early career' to 'established'.

Authors:

Biotechniques 2019 Jan;66(1):9-11

PhDs, postdocs, fellows and newly appointed lecturers all fall into the category of early-career researchers (ECRs). The life of researchers in early stages of their career is far from straightforward. Funding is a huge barrier for many ECRs looking to make the next step in their career, but it's not the only challenge. Read More

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http://dx.doi.org/10.2144/btn-2018-0182DOI Listing
January 2019
1 Read

The unsustainable lab.

Authors:
Abigail Sawyer

Biotechniques 2019 Jan;66(1):5-7

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http://dx.doi.org/10.2144/btn-2018-0185DOI Listing
January 2019
1 Read

Bead-based assay for spatiotemporal gene expression control in cell-free transcription-translation systems.

Biotechniques 2019 Jan;66(1):29-33

Universität des Saarlandes, Biologische Experimentalphysik, Naturwissenschaftlich-Technische Fakultät, B2 1, Campus, 66123 Saarbrücken, Germany.

Cell-free gene expression has applications in synthetic biology, biotechnology and biomedicine. In this technique gene expression regulation plays an important role. Transcription factors do not completely suppress expression while other methods for expression control, for example CRISPR/Cas, often require important biochemical modifications. Read More

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http://dx.doi.org/10.2144/btn-2018-0097DOI Listing
January 2019

A convenient method to generate and maintain poly(A)-encoding DNA sequences required for in vitro transcription of mRNA.

Biotechniques 2019 Jan;66(1):37-39

Wits/SAMRC Antiviral Gene Therapy Research Unit, School of Pathology, Faculty of Health Sciences, University of the Witwatersrand, Private Bag 3, WITS 2050, Johannesburg, South Africa.

Generating mRNA in vitro to encode therapeutic or cell-modifying proteins is rapidly gaining favor. An important factor that determines efficiency of translation from in vitro transcribed mRNA is the length of the 3' poly(A) sequence. However, reproducibly generating and maintaining templates from circular plasmids to have consistent lengths of the homo poly(A) sequences is challenging. Read More

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http://dx.doi.org/10.2144/btn-2018-0120DOI Listing
January 2019
1 Read

Assessment of cell yield among different devices for endovascular biopsy to harvest vascular endothelial cells.

Biotechniques 2019 Jan;66(1):34-36

Department of Radiology & Biomedical Imaging, Division of Neurointerventional Radiology, University of California - San Francisco, San Francisco, CA, USA.

Endovascular biopsy can increase understanding of vascular disease by granting access to epigenetic data that are not normally attainable. This study compares biopsy yields among multiple devices used, examining differences in cell counts according to species, device type, sampling location and disease state. Chi-square analysis compared means of cells harvested with respect to these variables. Read More

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http://dx.doi.org/10.2144/btn-2018-0099DOI Listing
January 2019
1 Read

A look back at 2018.

Biotechniques 2019 Jan;66(1):12-13

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http://dx.doi.org/10.2144/btn-2018-0181DOI Listing
January 2019
1 Read

Sample Collection / Preservation and DNA Isolation from stool samples for MICROBIOME analysi.

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Biotechniques 2019 Jan;66(1):54

Appropriate preservation and storage of stool samples is crucial to maintain DNA integrity and microbial community composition for downstream applications and analysis, including NGS and microbiome characterization. Read More

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http://dx.doi.org/10.2144/btn-2018-2014DOI Listing
January 2019

New year, new BioTechniques.

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Biotechniques 2019 Jan;66(1)

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https://www.future-science.com/doi/10.2144/btn-2018-0186
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http://dx.doi.org/10.2144/btn-2018-0186DOI Listing
January 2019
3 Reads

A method for rapid analysis of the root hydrotropic response in Arabidopsis thaliana.

Biotechniques 2019 Jan 11. Epub 2019 Jan 11.

Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Av. Universidad 2001, Col. Chamilpa, Cuernavaca, Mor., 62210, México.

The system for analyzing the hydrotropic curvature with a moisture gradient in wild-type Arabidopsis roots was modified. Optimal conditions were determined for detecting a hydrotropic curvature of 90° just after 4 h of stimulation. This system only requires 15 ml of a solution of KCO with a density of 1. Read More

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https://www.future-science.com/doi/10.2144/btn-2018-0173
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http://dx.doi.org/10.2144/btn-2018-0173DOI Listing
January 2019
8 Reads

Development of a motion-based cell-counting system for Trypanosoma parasite using a pattern recognition approach.

Biotechniques 2019 Apr 13;66(4):179-185. Epub 2018 Dec 13.

Artificial Intelligence Research Center, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan.

Automated cell counters that utilize still images of sample cells are widely used. However, they are not well suited to counting slender, aggregate-prone microorganisms such as Trypanosoma cruzi. Here, we developed a motion-based cell-counting system, using an image-recognition method based on a cubic higher-order local auto-correlation feature. Read More

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http://dx.doi.org/10.2144/btn-2018-0163DOI Listing
April 2019
3 Reads

Oxford nanopore sequencing enables rapid discovery of single-domain antibodies from phage display libraries.

Biotechniques 2018 12;65(6):351-356

Human Health Therapeutics Research Centre, National Research Council Canada, 100 Sussex Drive, Ottawa, Ontario, Canada, K1A 0R6.

Antibody (Ab) repertoire sequencing using high-throughput massively parallel technologies has contributed substantially to the understanding of Ab responses following infection, vaccination and autoimmunity. Because individual B-cell receptors are recombined and diversified somatically, genomic comparisons are limited, and distinguishing rare variants from sequencing errors is a major challenge. Oxford Nanopore Technologies' MinION is a highly portable and cost-effective third-generation sequencing instrument, but has not been used for Ab repertoire sequencing due to its high error rate (approximately 1/10 bases). Read More

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http://dx.doi.org/10.2144/btn-2018-0123DOI Listing
December 2018
3 Reads

Rapid selection of single-stranded DNA aptamers binding Staphylococcus epidermidis in platelet concentrates.

Biotechniques 2018 12;65(6):331-338

Division of Emerging & Transfusion-Transmitted Diseases, Laboratory of Bacterial & Transmissible Spongiform Encephalopathy Agents, Center for Biologics Evaluation & Research, Office of Blood Research & Review, US Food & Drug Administration, 10903 New Hampshire Avenue, Silver Spring, MD 20993, USA.

Staphylococcus epidermidis is the most common transfusion-associated pathogen contaminating platelet concentrates. Methods to reduce or eliminate contaminating bacteria from platelet units are critical for improving the safety of blood transfusions. We used rapid isolation of DNA aptamers (RIDA) to identify single-stranded (ss)DNA aptamers as ligands that specifically bind to S. Read More

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http://dx.doi.org/10.2144/btn-2018-0081DOI Listing
December 2018
3 Reads

Samasy: an automated system for sample selection and robotic transfer.

Biotechniques 2018 12;65(6):357-360

Department of Epidemiology & Biostatistics, University of California, San Francisco, CA, USA.

Sample automation and management is increasingly important as the number and size of population-scale and high-throughput projects grow. This is particularly the case in large-scale population studies where sample size is far outpacing the commonly used 96-well plate format. To facilitate management and transfer of samples in this format, we present Samasy, a web-based application for the construction of a sample database, intuitive display of sample and batch information, and facilitation of automated sample transfer or subset. Read More

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http://dx.doi.org/10.2144/btn-2018-0090DOI Listing
December 2018
3 Reads

DNA barcodes from snake venom: a broadly applicable method for extraction of DNA from snake venoms.

Biotechniques 2018 12;65(6):339-345

School of Biological Sciences, University of Northern Colorado, 501 20th Street, Greeley, CO 80639-0017, USA.

DNA barcoding is a simple technique used to develop a large-scale system of classification that is broadly applicable across a wide variety of taxa. DNA-based analysis of snake venoms can provide a system of classification independent of currently accepted taxonomic relationships by generating DNA barcodes specific to each venom sample. DNA purification from dried snake venoms has previously required large amounts of starting material, has resulted in low yields and inconsistent amplification, and was possible with front-fanged snakes only. Read More

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http://dx.doi.org/10.2144/btn-2018-0096DOI Listing
December 2018
1 Read

Updates from the Society for Neuroscience 2018.

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Biotechniques 2018 12;65(6):303

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http://dx.doi.org/10.2144/btn-2018-0170DOI Listing
December 2018
2 Reads

QuickCount: a novel automated software for rapid cell detection and quantification.

Biotechniques 2018 12;65(6):322-330

Head & Neck Cancer Research Team, Cancer Research Malaysia, Subang Jaya, Selangor, Malaysia.

We describe a novel automated cell detection and counting software, QuickCount (QC), designed for rapid quantification of cells. The Bland-Altman plot and intraclass correlation coefficient (ICC) analyses demonstrated strong agreement between cell counts from QC to manual counts (mean and SD: -3.3 ± 4. Read More

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https://www.future-science.com/doi/10.2144/btn-2018-0072
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http://dx.doi.org/10.2144/btn-2018-0072DOI Listing
December 2018
30 Reads
2.948 Impact Factor

Tech news: stem cells for modeling and curing disease.

Authors:
Freya Leask

Biotechniques 2018 12;65(6):305-310

Freya Leask explores developments in cell culture and stem cell research that are revolutionizing how diseases are studied and treated. Read More

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http://dx.doi.org/10.2144/btn-2018-0171DOI Listing
December 2018
2 Reads

3D-printed magnetic tweezers for dorsal traction force measurement.

Biotechniques 2018 12;65(6):347-349

Department of Mechanical Engineering, Johns Hopkins University, MD, USA.

In this study, economic magnetic tweezers (EMT) with a sharp gradient field were designed and built, in order to facilitate accurate force measurement. Our design costs less than 40 USD and is easy to mount onto most microscope stages. We leverage the computational fluidic dynamics techniques to calculate the forces based on the results obtained using our simple device. Read More

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http://dx.doi.org/10.2144/btn-2018-0078DOI Listing
December 2018
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Advances in cancer modeling: fluidic systems for increasing representativeness of large 3D multicellular spheroids.

Biotechniques 2018 12;65(6):312-314

Advanced Research Centre on Electronic Systems for Information & Communication Technologies 'E. De Castro' (ARCES), University of Bologna, Italy.

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http://dx.doi.org/10.2144/btn-2018-0153DOI Listing
December 2018
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Can a new microscopy platform help to improve clinical outcomes?

Biotechniques 2018 11;65(5):250-251

Department of Physics, Virginia Commonwealth University, Richmond, VA, USA.

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https://www.future-science.com/doi/10.2144/btn-2018-0102
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http://dx.doi.org/10.2144/btn-2018-0102DOI Listing
November 2018
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Chromium sequencing: the doors open for genomics of obligate plant pathogens.

Biotechniques 2018 11;65(5):253-257

Department of Biochemistry, Genetics & Microbiology, Tree Protection Co-operative Programme (TPCP), Forestry & Agricultural Biotechnology Institute (FABI), University of Pretoria, South Africa.

It is challenging to sequence and assemble genomes of obligate plant pathogens and microorganisms because of limited amounts of DNA, comparatively large genomes and high numbers of repeat regions. We sequenced the 1.2 gigabase genome of an obligate rust fungus, Austropuccinia psidii, the cause of rust on Myrtaceae, with a Chromium 10X library. Read More

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https://www.future-science.com/doi/10.2144/btn-2018-0019
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http://dx.doi.org/10.2144/btn-2018-0019DOI Listing
November 2018
11 Reads