46,646 results match your criteria BioScience [Journal]


Enhanced hypovirus transmission by engineered super donor strains of the chestnut blight fungus, Cryphonectria parasitica, into a natural population of strains exhibiting diverse vegetative compatibility genotypes.

Virology 2018 Dec 11;528:1-6. Epub 2018 Dec 11.

Division of Plant and Soil Sciences, West Virginia University, Morgantown, WV, USA. Electronic address:

Horizontal transmission of virulence attenuating hypoviruses of Cryphonectria parasitica is restricted by an allorecognition system termed vegetative incompatibility (vic). A super donor formulation of two engineered C. parasitica strains (SD328/SD82) with gene disruptions at four of six vic loci transmitted hypovirus to strains in the laboratory independent of vic genotype. Read More

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December 2018

Iron-associated protein interaction networks reveal the key functional modules related to survival and virulence of Pasteurella multocida.

Microb Pathog 2018 Dec 11. Epub 2018 Dec 11.

Department of Zoology, Faculty of Science, Kasetsart University, Bangkok, 10900, Thailand; Omics Center for Agriculture, Bioresources, Food, and Health, Faculty of Science, Kasetsart University (OmiKU), Bangkok, 10900, Thailand. Electronic address:

Pasteurella multocida causes respiratory infectious diseases in a multitude of birds and mammals. A number of virulence-associated genes were reported across different strains of P. multocida, including those involved in the iron transport and metabolism. Read More

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December 2018

Deletion the C-terminal peptides of Vibrio harveyi phospholipase D significantly improved its enzymatic properties.

Int J Biol Macromol 2018 Dec 11. Epub 2018 Dec 11.

School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China. Electronic address:

A novel phospholipase D that originate from marine Vibrio harveyi (VhPLD) was recombinant expressed and biochemically characterized. Moreover, effects of C-terminal peptides on catalytic and interfacial binding properties of VhPLD were investigated by constructing two truncated mutants (VhPLD-Δ(472-483) and VhPLD-Δ(437-483)). Optimal reaction temperature and pH value for wild-type VhPLD (VhPLD-WT) was 45 °C and pH 8. Read More

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December 2018

Effects of laser shock peening on the corrosion behavior and biocompatibility of a nickel-titanium alloy.

J Biomed Mater Res B Appl Biomater 2018 Dec 14. Epub 2018 Dec 14.

Department of Mechanical Engineering, University of Akron, Akron, Ohio, 44325.

Nickel-titanium (NiTi) alloy is an attractive material for biomedical implant applications. In this study, the effects of laser shock peening (LSP) on the biocompatibility, corrosion resistance, ion release rate and hardness of NiTi were characterized. The cell culture study indicated that the LSP-treated NiTi samples had lower cytotoxicity and higher cell survival rate than the untreated samples. Read More

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December 2018

Genome survey sequencing for the characterization of genetic background of Dracaena cambodiana and its defense response during dragon's blood formation.

PLoS One 2018 14;13(12):e0209258. Epub 2018 Dec 14.

Key Laboratory of Biology and Genetic Resources of Tropical Crops of Ministry of Agriculture and Rural Affairs, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan, People's Republic of China.

Dragon's blood collected from the genus Dracaena is used as a renowned traditional medicine in various cultures worldwide. However, the genetics of the genus Dracaena and the formation mechanism of dragon's blood remain poorly understood. Here, we generate the first draft genome reference assembly of an elite Chinese Dracaena species, Dracaena cambodiana, from next-generation sequencing data with 89. Read More

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December 2018

Real-time Visualization of Breast Carcinoma in Pathology Specimens From Patients Receiving Fluorescent Tumor-Marking Agent Tozuleristide.

Arch Pathol Lab Med 2018 Dec 14. Epub 2018 Dec 14.

From the Departments of Pathology (Dr Dintzis) and Surgery (Ms Hales and Drs Calhoun, Javid, and Byrd), University of Washington Medical Center, Seattle; Breast Surgery Clinic (Dr Harrington), Department of Pathology (Dr Tan), and Clinical Trials (Mses Fortney and Porenta), Overlake Hospital Medical Center, Bellevue, Washington; Development (Dr Miller), Clinical Operations (Mses Ishak and Gombotz), Research (Ms Hansen and Dr Parrish-Novak), and Device Development (Dr Kittle and Mr Perry), Blaze Bioscience, Inc., Seattle, Washington; and the Department of Surgery, University of Washington, Seattle (Dr Anderson).

Context.—: Resection of breast carcinoma with adequate margins reduces the risk of local recurrence and reoperation. Tozuleristide (BLZ-100) is an investigational peptide-fluorophore agent that may aid in intraoperative tumor detection and margin assessment. Read More

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December 2018

An antibiofilm coating of 5-aryl-2-aminoimidazole covalently attached to a titanium surface.

J Biomed Mater Res B Appl Biomater 2018 Dec 13. Epub 2018 Dec 13.

Department of Microbial and Molecular Systems, Centre of Microbial and Plant Genetics (CMPG), KU Leuven, Kasteelpark Arenberg 20 Box 2460, 3001 Leuven, Belgium.

Biofilms, especially those formed by Staphylococcus aureus, play a key role in the development of orthopedic implant infections. Eradication of these infections is challenging due to the elevated tolerance of biofilm cells against antimicrobial agents. In this study, we developed an antibiofilm coating consisting of 5-(4-bromophenyl)-N-cyclopentyl-1-octyl-1H-imidazol-2-amine, designated as LC0024, covalently bound to a titanium implant surface (LC0024-Ti). Read More

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December 2018
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Whole Mouse Brain Reconstruction and Registration to a Reference Atlas with Standard Histochemical Processing of Coronal Sections.

J Comp Neurol 2018 Dec 14:e24602. Epub 2018 Dec 14.

Section on Neuroanatomy, NIMH, Bethesda, MD.

Advances in molecular neuroanatomical tools have expanded the ability to map in detail connections of specific neuron subtypes in the context of behaviorally driven patterns of neuronal activity. Analysis of such data across the whole mouse brain, registered to a reference atlas, aids in understanding the functional organization of brain circuits related to behavior. A process is described to image mouse brain sections labeled with standard histochemical techniques, reconstruct those images into a whole brain image volume and register those images to the Allen Mouse Brain Common Coordinate Framework. Read More

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December 2018

Molecular signatures of increased freezing tolerance due to low temperature memory in Arabidopsis.

Plant Cell Environ 2018 Dec 12. Epub 2018 Dec 12.

Max-Planck-Institute of Molecular Plant Physiology, Am Mühlenberg 1, 14476, Potsdam, Germany.

Alternating temperatures require fast and coordinated adaptation responses of plants. Cold acclimation has been extensively investigated and results in increased freezing tolerance in Arabidopsis thaliana. Here we show that the two Arabidopsis accessions Col-0 and N14 that differ in their freezing tolerance, showed memory of cold acclimation, i. Read More

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December 2018

Diversified Sources for Human Infections by Salmonella enterica serovar Newport.

Transbound Emerg Dis 2018 Dec 11. Epub 2018 Dec 11.

CATG Microbiology & Food Safety Laboratory, Institute of Preventive Veterinary Medicine, Zhejiang University College of Animal Sciences, Zhejiang, 310058, Hangzhou, China.

Salmonella enterica Newport (S. Newport), with phylogenetic diversity feature, contributes to significant public health concerns. Our previous study suggested that S. Read More

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December 2018

Recombinant Expression and Bioactivity Comparison of Four Typical Fungal Immunomodulatory Proteins from Three Main Ganoderma Species.

BMC Biotechnol 2018 Dec 14;18(1):80. Epub 2018 Dec 14.

Liaoning Province Key Laboratory of Agricultural Technology, College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang, 110866, China.

Background: More than a dozen of fungal immunomodulatory proteins (FIPs) have been identified to date, most of which are from Ganoderma species. However, little is known about the similarities and differences between different Ganoderma FIPs' bioactivities. In the current study, two FIP genes termed FIP-gap1 and FIP-gap2 from G. Read More

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December 2018

Evaluating the profound effect of gut microbiome on host appetite in pigs.

BMC Microbiol 2018 Dec 14;18(1):215. Epub 2018 Dec 14.

State Key Laboratory of Pig Genetic Improvement and Production Technology, Jiangxi Agricultural University, Nanchang, 330045, China.

Background: There are growing evidences showing that gut microbiota should play an important role in host appetite and feeding behavior. However, what kind of microbe(s) and how they affect porcine appetite remain unknown.

Results: In this study, 280 commercial Duroc pigs were raised in a testing station with the circadian feeding behavior records for a continuous period of 30-100 kg. Read More

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December 2018

Variation in the effectiveness of insecticide treated nets against malaria and outdoor biting by vectors in Kilifi, Kenya.

Wellcome Open Res 2017 30;2:22. Epub 2017 Mar 30.

KEMRI-Wellcome Trust Research Programme, Centre for Geographic Medicine Research-Coast, Kilifi, Kenya.

Insecticide treated nets (ITNs) protect humans against bites from the mosquito vectors that transmit malaria, thereby reducing malaria morbidity and mortality. It has been noted that ITN use leads to a switch from indoor to outdoor feeding among these vectors. It might be expected that outdoor feeding would undermine the effectiveness of ITNs that target indoors vectors, but data are limited. Read More

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Editorial: Bioscience of D-amino Acid Oxidase From Biochemistry to Pathophysiology.

Front Mol Biosci 2018 28;5:108. Epub 2018 Nov 28.

Department of Pharmacology, Keio University School of Medicine, Tokyo, Japan.

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November 2018

Weight loss as a prognostic factor for recurrence and survival in oropharyngeal squamous cell carcinoma patients.

Mol Clin Oncol 2018 Dec 5;9(6):666-672. Epub 2018 Oct 5.

Oncology Research Group, Santa Terezinha University Hospital, Joaçaba, SC 89600-000, Brazil.

Although the mortality rate of oropharyngeal squamous cell carcinoma (OPSCC) has been decreasing over the last 30 years, there has been a significant increase in the frequency of diagnosis of this type of cancer in several countries. Beyond the classic prognostic factors, such as TNM stage, there is a lack of predictive factors for recurrence and treatment response. A retrospective analysis of patients with OPSCC treated at the Oncology Department of the Santa Terezinha University Hospital between 2007 and 2012 was performed, with the aim of identifying new prognostic factors. Read More

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December 2018

Differential gene expression in human tissue resident regulatory T cells from lung, colon, and blood.

Oncotarget 2018 Nov 16;9(90):36166-36184. Epub 2018 Nov 16.

Bioscience, Respiratory, Inflammation and Autoimmunity, Innovative Medicines and Early Development Biotech Unit, AstraZeneca, Gothenburg, Sweden.

As we learn more about how immune responses occur , it is becoming clear that each organ/tissue is characterized with its own anatomy and microenvironment which may affect and even determine the outcome of the immune responses. With emerging data from animal studies showing that regulatory T cells infiltrating non-lymphoid tissues exhibit unique phenotypes and transcriptional signatures and display functions beyond their well-established suppressive roles, there is an urgent need to explore the function of tissue Treg cells in humans. Here we characterized the transcriptome of Treg residing at the human mucosal tissue obtained from the normal area of cancer resections and their peripheral blood counterparts, identifying human lung and colon tissue Treg signature genes and their upstream regulators. Read More

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November 2018

Phospholipase A in skin biology: new insights from gene-manipulated mice and lipidomics.

Inflamm Regen 2018 7;38:31. Epub 2018 Dec 7.

1Laboratory of Microenvironmental and Metabolic Health Science, Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8655 Japan.

The skin represents one of the tissues that are most profoundly influenced by alterations in the quality of lipids (lipoquality). Lipids not only constitute cellular membranes, but also serve as bioactive lipid mediators and essential components of the skin barrier. Phospholipase A (PLA) enzymes supply fatty acids and lysophospholipids from membrane phospholipids, thereby variably affecting cutaneous homeostasis. Read More

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December 2018

Life-history changes in the cold tolerance of the two-spot spider mite : applications in pest control and establishment risk assessment.

Physiol Entomol 2018 Dec 12;43(4):334-345. Epub 2018 Sep 12.

School of Bioscience University of Birmingham Birmingham U.K.

Lethal time (LTime) and lethal temp (LTemp) are commonly used laboratory indices of arthropod cold tolerance, with the former often being employed to predict winter survival in the field. In the present study, we compare the cold tolerance of different life-history stages (nondiapausing and diapausing females, as well as males and juveniles) of a major agricultural pest: the two-spot spider mite Koch (Acarina: Tetranychidae). Diapausing females from European populations of this species are shown to be freeze avoiding, supercooling to -23. Read More

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December 2018

Characterisation of small molecule ligands 4CMTB and 2CTAP as modulators of human FFA2 receptor signalling.

Sci Rep 2018 Dec 13;8(1):17819. Epub 2018 Dec 13.

Institute for Molecular Bioscience, The University of Queensland, Brisbane, Australia.

Short chain fatty acids (SCFAs) are protective against inflammatory diseases. Free fatty acid receptor 2 (FFA2), is a target of SCFAs however, their selectivity for FFA2 over other FFA receptors is limited. This study aimed to functionally characterise 2-(4-chlorophenyl)-3-methyl-N-(thiazole-2-yl)butanamide (4CMTB) and 4-((4-(2-chlorophenyl)thiazole-2-yl)amino)-4oxo-3-phenylbutanoic acid (2CTAP), and their enantiomers, in modulating FFA2 activity. Read More

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December 2018

Asxl1 ablation in mouse embryonic stem cells impairs neural differentiation without affecting self-renewal.

Biochem Biophys Res Commun 2018 Dec 10. Epub 2018 Dec 10.

Department of Integrative Bioscience & Biotechnology, Sejong University, Seoul 05006, South Korea. Electronic address:

Additional sex comb-like1 (Asxl1) is known as a chromatin modulator that plays dual functions in transcriptional regulation depending on the cell type. Recent studies using Asxl1 knockout mice revealed that Asxl1 is important for the proliferation and differentiation of hematopoietic progenitor cells, and the development of organs. Although we previously reported Asxl1 as a Sox2 target gene, its function in embryonic stem cells (ESCs) remains largely unknown. Read More

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December 2018

Tat-ATOX1 inhibits inflammatory responses via regulation of MAPK and NF-κB pathways.

BMB Rep 2018 Dec 14. Epub 2018 Dec 14.

Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University, Chuncheon 24252, Korea.

Antioxidant 1 (ATOX1) protein has been reported to exhibit various protective functions, including antioxidant and chaperone. However, the effects of ATOX1 on the inflammatory response has not been fully elucidated. Thus, we prepared cell permeable Tat-ATOX1 and studied the effects on lipopolysaccharide (LPS)- and 12-O-tetradecanoyl phorbol-13- acetate (TPA)-induced inflammation. Read More

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December 2018
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SETDB1 regulates SMAD7 expression for breast cancer metastasis.

BMB Rep 2018 Dec 14. Epub 2018 Dec 14.

Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-333, Republic of Korea; Department of Functional Genomics, Korea University of Science and Technology, Daejeon, 305-333, Republic of Korea.

Breast cancer (BRC) is the most invasive cancer in women. Although the survival rate of BRC is gradually increasing due to improved screening systems, development of novel therapeutic targets for inhibition of BRC proliferation, metastasis and recurrence have been constantly needed. Thus, in this study, we identified overexpression of SETDB1 (SET Domain Bifurcated 1), a histone methyltransferase, in RNA-seq data of BRC derived from TCGA portal. Read More

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December 2018

The 9th Santorini Conference: Systems Medicine, Personalised Health and Therapy. "The Odyssey from Hope to Practice", Santorini, Greece, 30 September⁻3 October 2018.

J Pers Med 2018 Dec 12;8(4). Epub 2018 Dec 12.

PRAHealthSciences, Salt Lake City, UT 84124, USA.

The 9th traditional biannual conference on Systems Medicine, Personalised Health & Therapy-"The Odyssey from Hope to Practice", inspired by the Greek mythology, was a call to search for practical solutions in cardio-metabolic diseases and cancer, to resolve and overcome the obstacles in modern medicine by creating more interactions among disciplines, as well as between academic and industrial research, directed towards an effective 'roadmap' for personalised health and therapy. The 9th Santorini Conference, under the Presidency of Sofia Siest, the director of the INSERM U1122; IGE-PCV (www.u1122. Read More

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December 2018

A Native Human Monoclonal Antibody Targeting HCMV gB (AD-2 Site I).

Int J Mol Sci 2018 Dec 11;19(12). Epub 2018 Dec 11.

Trellis Bioscience, LLC, 702 Marshall Street, Suite 614, Redwood City, CA 94063, USA.

Hyperimmune globulin (HIG) has shown efficacy against human cytomegalovirus (HCMV) for both transplant and congenital transmission indications. Replicating that activity with a monoclonal antibody (mAb) offers the potential for improved consistency in manufacturing, lower infusion volume, and improved pharmacokinetics, as well as reduced risk of off-target reactivity leading to toxicity. HCMV pathology is linked to its broad cell tropism. Read More

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December 2018

Effects of Toona sinensis Leaf Extract and Its Chemical Constituents on Xanthine Oxidase Activity and Serum Uric Acid Levels in Potassium Oxonate-Induced Hyperuricemic Rats.

Molecules 2018 Dec 9;23(12). Epub 2018 Dec 9.

Herbal Medicine Research Division, Korea Institute of Oriental Medicine (KIOM), Daejeon 34054, Korea.

leaf is used as a seasonal vegetable in Korea. A 70% ethanol extract of these leaves exhibited potent xanthine oxidase (XO) inhibition, with a 50% inhibitory concentration (IC) of 78.4 µM. Read More

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December 2018

The Epigenetic Regulation of HCC Metastasis.

Int J Mol Sci 2018 Dec 10;19(12). Epub 2018 Dec 10.

Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Korea.

Epigenetic alterations, such as histone modification, DNA methylation, and miRNA-mediated processes, are critically associated with various mechanisms of proliferation and metastasis in several types of cancer. To overcome the side effects and limited effectiveness of drugs for cancer treatment, there is a continuous need for the identification of more effective drug targets and the execution of mechanism of action (MOA) studies. Recently, epigenetic modifiers have been recognized as important therapeutic targets for hepatocellular carcinoma (HCC) based on their reported abilities to suppress HCC metastasis and proliferation in both in vivo and in vitro studies. Read More

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December 2018

Multiple Exon Skipping in the Duchenne Muscular Dystrophy Hot Spots: Prospects and Challenges.

J Pers Med 2018 Dec 7;8(4). Epub 2018 Dec 7.

Department of Medical Genetics, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, AB T6G2H7, Canada.

Duchenne muscular dystrophy (DMD), a fatal X-linked recessive disorder, is caused mostly by frame-disrupting, out-of-frame deletions in the () gene. Antisense oligonucleotide-mediated exon skipping is a promising therapy for DMD. Exon skipping aims to convert out-of-frame mRNA to in-frame mRNA and induce the production of internally-deleted dystrophin as seen in the less severe Becker muscular dystrophy. Read More

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December 2018

The Power of Fish Models to Elucidate Skin Cancer Pathogenesis and Impact the Discovery of New Therapeutic Opportunities.

Int J Mol Sci 2018 Dec 7;19(12). Epub 2018 Dec 7.

Department of Chemistry, Chung Yuan Christian University, Chung-Li 32023, Taiwan.

Animal models play important roles in investigating the pathobiology of cancer, identifying relevant pathways, and developing novel therapeutic tools. Despite rapid progress in the understanding of disease mechanisms and technological advancement in drug discovery, negative trial outcomes are the most frequent incidences during a Phase III trial. Skin cancer is a potential life-threatening disease in humans and might be medically futile when tumors metastasize. Read More

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December 2018

Molecular and Functional Interaction of the Myokine Irisin with Physical Exercise and Alzheimer's Disease.

Molecules 2018 Dec 7;23(12). Epub 2018 Dec 7.

Department of Rehabilitation Science, Graduate School of Inje University, Gimhae 50834, Korea.

Irisin, a skeletal muscle-secreted myokine, produced in response to physical exercise, has protective functions in both the central and the peripheral nervous systems, including the regulation of brain-derived neurotrophic factors. In particular, irisin is capable of protecting hippocampus. Since this area is the region of the brain that is most susceptible to Alzheimer's disease (AD), such beneficial effect may inhibit or delay the emergence of neurodegenerative diseases, including AD. Read More

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December 2018

Efficient interleukin-21 production by optimization of codon and signal peptide in Chinese hamster ovarian cells.

J Microbiol Biotechnol 2018 Dec 7. Epub 2018 Dec 7.

Immunotherapy Convergence Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Republic of Korea.

Interleukin-21 is a common γ-chain cytokine that controls the immune responses of B cells, T cells, and natural killer cells. Targeting IL-21 to strengthen the immune system is promising for the development of vaccines as well as anti-infection and anti-tumor therapies. However, the practical application of IL-21 is limited by the high production cost. Read More

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December 2018

Neither n-3 Long-Chain PUFA Supplementation of Mothers through Lactation nor of Offspring in a Complementary Food Affects Child Overall or Social-Emotional Development: A 2 × 2 Factorial Randomized Controlled Trial in Rural Ethiopia.

J Nutr 2018 Dec 12. Epub 2018 Dec 12.

Department of Food Technology, Safety, and Health, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium.

Background: The n-3 (ω-3) long-chain polyunsaturated fatty acid (LC-PUFA) docosahexaenoic acid (DHA) is essential for optimal brain development. There is a lack of evidence on the effect of postnatal n-3 LC-PUFA supplementation on child development in low-income countries.

Objective: We evaluated the efficacy of fish-oil supplementation through lactation or complementary food supplementation on the development of children aged 6-24 mo in rural Ethiopia. Read More

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December 2018

SIZ1-Mediated SUMOylation of TPR1 Suppresses Plant Immunity in Arabidopsis.

Mol Plant 2018 Dec 11. Epub 2018 Dec 11.

Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China. Electronic address:

Plant immune responses are tightly regulated to ensure their appropriate deployment. The overexpression of TOPLESS-RELATED 1 (TPR1), a SUPPRESSOR OF npr1-1, CONSTITUTIVE 1 (SNC1)-interacting protein, results in autoimmunity, which reduces plant growth and development. However, how TPR1 activity is regulated remains unknown. Read More

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December 2018

Elastic recovery of filler-binders to safeguard viability of Lactobacillus rhamnosus GG during direct compression.

Eur J Pharm Biopharm 2018 Dec 10. Epub 2018 Dec 10.

University of Antwerp, Department of Pharmaceutical, Biomedical and Veterinary Sciences, Laboratory of Pharmaceutical Technology and Biopharmacy, Universiteitsplein 1, B-2610 Wilrijk, Belgium. Electronic address:

Tablets are increasingly explored as dosage form for oral probiotics, especially for applications such as pharyngitis and dental health. In such tablets, the dry form increases the stability and the shelf life of the product. In addition, the probiotic cells are entrapped in the tablet matrix, which protects them against the environmental factors in the human body. Read More

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December 2018

Small cell lung cancer transformation as a mechanism of resistance to PD-1 therapy in KRAS mutant lung adenocarcinoma: A report of two cases.

J Thorac Oncol 2018 Dec 10. Epub 2018 Dec 10.

Department of Medicine, Division of Hematology-Oncology, Vanderbilt University Medical Center; Vanderbilt-Ingram Cancer Center, Nashville, TN 37232, USA. Electronic address:

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December 2018

Versatile spider venom peptides and their medical and agricultural applications.

Toxicon 2018 Dec 10. Epub 2018 Dec 10.

Institute for Molecular Bioscience, The University of Queensland, St. Lucia, QLD 4072, Australia. Electronic address:

Spiders have been evolving complex and diverse repertoires of peptides in their venoms with vast pharmacological activities for more than 300 million years. Spiders use their venoms for prey capture and defense, hence they contain peptides that target both prey (mainly arthropods) and predators (other arthropods or vertebrates). This includes peptides that potently and selectively modulate a range of targets such as ion channels, receptors and signaling pathways involved in physiological processes. Read More

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December 2018

Direct visualization of single-molecule membrane protein interactions in living cells.

PLoS Biol 2018 Dec 13;16(12):e2006660. Epub 2018 Dec 13.

Department of Life Sciences, Pohang University of Science and Technology, Pohang, Republic of Korea.

Interactions between membrane proteins are poorly understood despite their importance in cell signaling and drug development. Here, we present a co-immunoimmobilization assay (Co-II) enabling the direct observation of membrane protein interactions in single living cells that overcomes the limitations of currently prevalent proximity-based indirect methods. Using Co-II, we investigated the transient homodimerizations of epidermal growth factor receptor (EGFR) and beta-2 adrenergic receptor (β2-AR) in living cells, revealing the differential regulation of these receptors' dimerizations by molecular conformations and microenvironment in a plasma membrane. Read More

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December 2018

Positioning High-Throughput CETSA in Early Drug Discovery through Screening against B-Raf and PARP1.

SLAS Discov 2018 Dec 13:2472555218813332. Epub 2018 Dec 13.

1 Discovery Biology, Discovery Sciences, IMED Biotech Unit, AstraZeneca, Cambridge, UK.

Methods to measure cellular target engagement are increasingly being used in early drug discovery. The Cellular Thermal Shift Assay (CETSA) is one such method. CETSA can investigate target engagement by measuring changes in protein thermal stability upon compound binding within the intracellular environment. Read More

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December 2018

Development of Transgenic CryIA(c) + GNA Cotton Plants via Pollen-Tube Pathway Method Confers Resistance to Helicoverpa armigera and Aphis gossypii Glover.

Methods Mol Biol 2019 ;1902:233-244

Agronomy Department, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, Zhejiang, China.

Elite cotton cultivar Sumian16 was transformed with p7RPSBK-mGNA-NPTII containing Bt (CryIA(c)), Galanthus nivalis agglutinin (GNA) resistance genes, and selectable marker NptII gene via the pollen-tube pathway method, and two fertile transgenic Bt + GNA plants were obtained in the present study. The integration and expression of the Bt and GNA genes were confirmed by molecular biology techniques and insect bioassays. Insect bioassays showed that the transformed plants were highly toxic to bollworm larvae as well as obviously retarding development of aphid populations. Read More

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January 2019

Cotton Hairy Root Culture as an Alternative Tool for Cotton Functional Genomics.

Authors:
Hee Jin Kim

Methods Mol Biol 2019 ;1902:213-221

Cotton Fiber Bioscience Research Unit, USDA-ARS, Southern Regional Research Center, New Orleans, LA, USA.

Although well-accepted as the ultimate method for cotton functional genomics, Agrobacterium tumefaciens-mediated cotton transformation is not widely used for functional analyses of cotton genes and their promoters since regeneration of cotton in tissue culture is lengthy and labor intensive. In certain cases, A. rhizogenes-induced hairy root culture has been a suitable molecular tool for functional analyses of genes and promoters for plants that are difficult to regenerate by A. Read More

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January 2019

Red yeast rice fermentation with Bacillus subtilis B2 under blue light-emitting diodes increases antioxidant secondary products (Manuscript ID: BPBSE-18-0387).

Bioprocess Biosyst Eng 2018 Dec 12. Epub 2018 Dec 12.

Division of Biotechnology, Advanced Institute of Environment and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan, Jeonbuk, 54596, South Korea.

Light and bacteria can be used in combination to enhance secondary metabolite production during fermentation. Red yeast rice powder (RYRP) was inoculated with Bacillus subtilis (B2) isolated from freshwater seafood and incubated under light-emitting diodes (LEDs) of different colors (blue, green, red, white), fluorescent white light, and in darkness. Blue LED-mediated fermentation with B2 significantly enhanced production of phenolic compounds (68. Read More

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December 2018

Characterization of Hydrocarbon-Degrading Bacteria in Constructed Wetland Microcosms Used to Treat Crude Oil Polluted Water.

Bull Environ Contam Toxicol 2018 Dec 12. Epub 2018 Dec 12.

Department of Bioscience, Aarhus University Centre for Water Technology (WATEC), Aarhus University, 8000, Århus C, Denmark.

Ten plant species were grown in constructed wetlands (CWs) to remediate water containing 2% (w/v) crude oil. The plant species with better growth and biomass production were Typha latifolia and Cyperus laevigatus, and they were significantly correlated (R = 0.91) with hydrocarbon degradation. Read More

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December 2018

Tissue and host species-specific transcriptional changes in models of experimental visceral leishmaniasis.

Wellcome Open Res 2018 29;3:135. Epub 2018 Oct 29.

Centre for Immunology and Infection, University of York, York, YO10 5DD, UK.

: Human visceral leishmaniasis, caused by infection with or is a potentially fatal disease affecting 50,000-90,000 people yearly in 75 disease endemic countries, with more than 20,000 deaths reported. Experimental models of infection play a major role in understanding parasite biology, host-pathogen interaction, disease pathogenesis, and parasite transmission. In addition, they have an essential role in the identification and pre-clinical evaluation of new drugs and vaccines. Read More

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October 2018

Computational genome-wide identification of heat shock protein genes in the bovine genome.

F1000Res 2018 20;7:1504. Epub 2018 Sep 20.

International Programs, College of Agriculture and Life Sciences, Cornell University, Ithaca, NY, 14853, USA.

Heat shock proteins (HSPs) are molecular chaperones known to bind and sequester client proteins under stress. To identify and better understand some of these proteins, we carried out a computational genome-wide survey of the bovine genome. For this, HSP sequences from each subfamily (sHSP, HSP40, HSP70 and HSP90) were used to search the Pfam (Protein family) database, for identifying exact HSP domain sequences based on the hidden Markov model. Read More

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September 2018

Baculovirus as an efficient vector for gene delivery into mosquitoes.

Sci Rep 2018 Dec 12;8(1):17778. Epub 2018 Dec 12.

Institute of Molecular Biology, Academia Sinica, No. 128, Sec. 2, Academia Road, Nankang, Taipei, 115, Taiwan, Republic of China.

Efficient gene delivery technologies play an essential role in the gene functional analyses that are necessary for basic and applied researches. Mosquitoes are ubiquitous insects, responsible for transmitting many deadly arboviruses causing millions of human deaths every year. The lack of efficient and flexible gene delivery strategies in mosquitoes are among the major hurdles for the study of mosquito biology and mosquito-pathogen interactions. Read More

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December 2018
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Mannonerolidol, a new nerolidol mannoside from culture broth of Schizophyllum commune.

J Antibiot (Tokyo) 2018 Dec 12. Epub 2018 Dec 12.

Division of Biotechnology and Advanced Institute of Environmental and Bioscience, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan-si, Korea.

To discover antimicrobial agents from higher fungi, mannonerolidol (3), a new nerolidol mannoside, together with known schizostatin (1) and nerolidol (2) were isolated from an antimicrobial fraction of the culture broth of Schizophyllum commune. Structures of these compounds were determined through spectroscopic methods. Compounds 1 and 3 exhibited antimicrobial activities against plant pathogenic fungi Rhizoctonia solani, Diaporthe sp. Read More

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December 2018

Rotational direction of flagellar motor from the conformation of FliG middle domain in marine Vibrio.

Sci Rep 2018 Dec 12;8(1):17793. Epub 2018 Dec 12.

Division of Biological Science, Graduate School of Science, Nagoya University, Chikusa-ku, Nagoya, 464-8602, Japan.

FliG, which is composed of three distinctive domains, N-terminal (N), middle (M), and C-terminal (C), is an essential rotor component that generates torque and determines rotational direction. To determine the role of FliG in determining flagellar rotational direction, we prepared rotational biased mutants of fliG in Vibrio alginolyticus. The E144D mutant, whose residue is belonging to the EHPQR-motif in FliG, exhibited an increased number of switching events. Read More

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December 2018

Reduced TCA cycle rates at high hydrostatic pressure hinder hydrocarbon degradation and obligate oil degraders in natural, deep-sea microbial communities.

ISME J 2018 Dec 12. Epub 2018 Dec 12.

Center for Microbial Ecology and Technology (CMET), Gent University, Coupure Links 653, Gent, B 9000, Belgium.

Petroleum hydrocarbons reach the deep-sea following natural and anthropogenic factors. The process by which they enter deep-sea microbial food webs and impact the biogeochemical cycling of carbon and other elements is unclear. Hydrostatic pressure (HP) is a distinctive parameter of the deep sea, although rarely investigated. Read More

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December 2018

MicroRNA amplification and detection technologies: opportunities and challenges for point of care diagnostics.

Lab Invest 2018 Dec 12. Epub 2018 Dec 12.

Laboratory of Applied Micro and Nanotechnology (LAMINATE), Division of Microbiology and Production, National Food Institute, Technical University of Denmark, 2800 Kgs, Lyngby, Denmark.

The volume of point of care (POC) testing continues to grow steadily due to the increased availability of easy-to-use devices, thus making it possible to deliver less costly care closer to the patient site in a shorter time relative to the central laboratory services. A novel class of molecules called microRNAs have recently gained attention in healthcare management for their potential as biomarkers for human diseases. The increasing interest of miRNAs in clinical practice has led to an unmet need for assays that can rapidly and accurately measure miRNAs at the POC. Read More

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December 2018

Comparative transcriptome analysis revealed the improved β-carotene production in Sporidiobolus pararoseus yellow mutant MuY9.

J Gen Appl Microbiol 2018 Dec 13. Epub 2018 Dec 13.

College of Land and Environment, Shenyang Agricultural University.

Carotenoids are ubiquitous constituents of living organisms. These structurally diverse pigments have received considerable attention due to their biotechnological applications and potential beneficial effects on human health. In this study, we characterized an over-producing β-carotene mutant of Sporidiobolus pararoseus, obtained by ultraviolet mutagenesis, named MuY9. Read More

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December 2018

Genetic diversity of 21 experimental chicken lines with diverse origins and genetic backgrounds.

Exp Anim 2018 Dec 13. Epub 2018 Dec 13.

Avian Bioscience Research Center, Graduate School of Bioagricultural Sciences, Nagoya University.

The genetic characteristics and diversity of 21 experimental chicken lines registered with the National BioResource Project of Japan were examined using mitochondrial D-loop sequences and 54 microsatellite DNA markers. A total of 12 haplotypes were detected in the 500-bp mitochondrial DNA sequences of the hypervariable segment I for 349 individuals of 21 lines. The 12 haplotypes belonged to three (A, D, and E) haplogroups, out of the seven (A-H) common haplogroups in domestic chickens and red junglefowls. Read More

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December 2018