16 results match your criteria BioNanoScience[Journal]

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Immunosuppressant Peptide Abu-TGIRIS-Abu-NH and its Application for Treatment of Multiple Sclerosis.

Bionanoscience 2018 2;8(1):484-489. Epub 2018 Mar 2.

1 Laboratory of Protein Chemistry, Branch of Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Prospekt Nauki, 6, Pushchino, 142290 Russia.

Immunosuppressant peptide immunocortin for the first time was described in 1993. It corresponds to residues 11-20 of human Ig heavy chain (conserved motif of V domain). There are no data about production of immunocortin by proteolysis of Ig in vivo. Read More

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http://dx.doi.org/10.1007/s12668-018-0513-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5866264PMC
March 2018
2 Reads

Toxicity and Applications of Internalised Magnetite Nanoparticles Within Live Cells.

Bionanoscience 2018 22;8(1):90-94. Epub 2017 Jun 22.

Unconventional Computing Laboratory, University of the West of England, Bristol, UK.

The nanotechnology revolution has allowed us to speculate on the possibility of hybridising nanoscale materials with live substrates, yet significant doubt still remains pertaining to the effects of nanomaterials on biological matter. In this investigation, we cultivate the ciliated protistic pond-dwelling microorganism in the presence of excessive quantities of magnetite nanoparticles in order to deduce potential beneficial applications for this technique, as well as observe any deleterious effects on the organisms' health. Our findings indicate that this variety of nanoparticle is well-tolerated by cells, who were observed to consume them in quantities exceeding 5-12% of their body volume: cultivation in the presence of magnetite nanoparticles does not alter cell volume, swimming speed, growth rate or peak colony density and cultures may persist in nanoparticle-contaminated media for many weeks. Read More

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http://link.springer.com/10.1007/s12668-017-0425-z
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http://dx.doi.org/10.1007/s12668-017-0425-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5866280PMC
June 2017
4 Reads

Towards One-Step Quantitation of Prostate-Specific Antigen (PSA) in Microfluidic Devices: Feasibility of Optical Detection with Nanoparticle Labels.

Bionanoscience 2017 7;7(4):718-726. Epub 2017 Jan 7.

Department of Chemical Engineering, Loughborough University, Loughborough, LE11 3TU UK.

Rapid and quantitative prostate-specific antigen (PSA) biomarker detection would be beneficial to cancer diagnostics, improving early detection and therefore increasing chances of survival. Nanoparticle-based detection is routinely used in one-step nitrocellulose-based lateral flow (LF) immunoassays; however, it is well established within the scientific diagnostic community that LF technology lacks sensitivity for measuring biomarkers, such as prostate-specific antigen (PSA). A trend in point-of-care (POC) protein biomarker quantitation is the miniaturization of immunoassays in microfluidic devices. Read More

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http://dx.doi.org/10.1007/s12668-016-0390-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5698394PMC
January 2017
1 Read

Single-Molecule Interactions of a Monoclonal Anti-DNA Antibody with DNA.

Bionanoscience 2017 Mar 11;7(1):132-147. Epub 2016 Oct 11.

Department of Cell and Developmental Biology, University of Pennsylvania School of Medicine, 421 Curie Boulevard, Philadelphia, PA 19104, USA.

Interactions of DNA with proteins are essential for key biological processes and have both a fundamental and practical significance. In particular, DNA binding to anti-DNA antibodies is a pathogenic mechanism in autoimmune pathology, such as systemic lupus erythematosus. Here we measured at the single-molecule level binding and forced unbinding of surface-attached DNA and a monoclonal anti-DNA antibody MRL4 from a lupus erythematosus mouse. Read More

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http://dx.doi.org/10.1007/s12668-016-0303-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5667910PMC
March 2017
5 Reads

Selection of efficient Taq DNA polymerase to optimize T-DNA genotyping method for rapid detection of mutant plants.

Bionanoscience 2016 Dec 27;6(4):407-410. Epub 2016 Aug 27.

Laboratory of Microbial Biotechnology, Institute of Fundamental Medicine and Biology, Kazan Federal University, 9 Parizhskaya Communa str., Kazan, 420021, Russia; Department of Integrative Biology, University of Texas at Austin, Austin, 78712, TX, USA.

Plants harbor homologues of various animal genes involved in phosphorus metabolism, telomere biology and other cellular processes. Compared to experiments with many other multicellular organisms, research in the model plant takes advantage of short generation time and an ever increasing arsenal of genetic and transgenic tools, including large collections of T-DNA knockout and activation lines. The availability of thousands of publicly available transgenic lines provides a unique opportunity to address a number of important biological questions. Read More

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http://dx.doi.org/10.1007/s12668-016-0253-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5295645PMC
December 2016
5 Reads

Non-radioactive TRF assay modifications to improve telomeric DNA detection efficiency in plants.

Bionanoscience 2016 Dec 6;6(4):325-328. Epub 2016 Aug 6.

Laboratory of Microbial Biotechnology, Institute of Fundamental Medicine and Biology, Kazan Federal University, 9 Parizhskaya Communa str., Kazan, 420021, Russia; Department of Integrative Biology, University of Texas at Austin, Austin, 78712, TX, USA.

The length of telomeric DNA is often considered a cellular biomarker of aging and general health status. Several telomere length measuring assays have been developed, of which the most common is the Telomere Restriction Fragment (TRF) analysis, which typically involves the use of radioactively labeled oligonucleotide probes. While highly effective, this method potentially poses substantial health concerns and generates radioactive waste. Read More

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http://dx.doi.org/10.1007/s12668-016-0223-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5268086PMC
December 2016
15 Reads

Magnetic Nanodrug Delivery Through the Mucus Layer of Air-Liquid Interface Cultured Primary Normal Human Tracheobronchial Epithelial Cells.

Bionanoscience 2016 Sep 28;6(3):235-242. Epub 2016 Jul 28.

Center of the BioFrontiers Institute, University of Colorado at Colorado Springs, 1420 Austin Bluffs Parkway, Colorado Springs, CO 80918, USA ; Department of Physics and Energy Science, University of Colorado at Colorado Springs, Colorado Springs, CO 80918, USA.

Superparamagnetic iron oxide (FeO) and highly anisotropic barium hexaferrite (BaFeO) nanoparticles were coated with an anti-inflammatory drug and magnetically transported through mucus produced by primary human airway epithelial cells. Using wet planetary ball milling, dl-2-amino-3-phosphonopropionic acid-coated BaFeO nano-particles (BaNPs) of 1-100 nm in diameter were prepared in water. BaNPs and conventional 20-30-nm FeO nanoparticles (FeNPs) were then encased in a polymer (PLGA) loaded with dexamethasone (Dex) and tagged for imaging. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5070545PMC
http://dx.doi.org/10.1007/s12668-016-0216-yDOI Listing
September 2016
2 Reads

The Effect of Polysialic Acid Expression on Glioma Cell Nano-mechanics.

Bionanoscience 2016;6:81-84. Epub 2016 Jan 25.

Institute of Cancer Therapeutics, Faculty of Life Sciences, University of Bradford, Bradford, BD7 1DP UK.

Polysialic acid (polySia) is an important carbohydrate bio-polymer that is commonly over-expressed on tumours of neuroendocrine origin and plays a key role in tumour progression. polySia exclusively decorates the neural cell adhesion molecule (NCAM) on tumour cell membranes, modulating cell-cell interactions, motility and invasion. In this preliminary study, we examine the nano-mechanical properties of isogenic C6 rat glioma cells-transfected cells engineered to express the enzyme polysialyltransferase ST8SiaII, which synthesises polySia (C6-STX cells) and wild-type cells (C6-WT). Read More

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http://dx.doi.org/10.1007/s12668-016-0192-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4778150PMC
January 2016
3 Reads

Discrimination Between Normal and Cancerous Cells Using AFM.

Bionanoscience 2016;6:65-80. Epub 2016 Jan 30.

Institute of Nuclear Physics, PAS, Radzikowskiego 152, 31-342 Kraków, Poland.

Currently, biomechanics of living cells is in the focus of interest due to noticeable capability of such techniques like atomic force microscopy (AFM) to probe cellular properties at the single cell level directly on living cells. The research carried out, so far, delivered data showing, on the one hand, the use of cellular mechanics as a biomarker of various pathological changes, which, on the other hand, reveal relative nature of biomechanics. In the AFM, the elastic properties of living cells are delivered from indentation experiments and described quantitatively by Young's modulus defined here as a measure of cellular deformability. Read More

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http://dx.doi.org/10.1007/s12668-016-0191-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4778153PMC
January 2016

Creating a Single Sensing Zone within an Alpha-Hemolysin Pore Via Site Directed Mutagenesis.

Bionanoscience 2014 Mar;4(1):78-84

Electronic BioSciences, Inc., 5754 Pacific Center Blvd. Suite 204, San Diego, CA 92121.

Although significant progress has recently been made towards realizing the goal of direct nanopore based DNA sequencing [1], there are still numerous hurdles that need to be overcome. One such hurdle associated with the use of the biological nanopore α-hemolysin (αHL) is the fact that the wild type channel contains three very distinct recognition or sensing regions within the β-barrel [2, 3], making identification of the bases residing within or moving through the pore very difficult. Through site directed mutagenesis, we have been able to selectively remove one of two sensing regions while simultaneously enhancing the third. Read More

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http://dx.doi.org/10.1007/s12668-013-0119-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3963172PMC
March 2014
2 Reads

Imaging of Protein Secretion from a Single Cell Using Plasmonic Substrates.

Bionanoscience 2013 Mar;3(1):30-36

Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, 725 W. Lombard St., Baltimore, MD, 21201, USA.

Detecting, imaging, and monitoring cell function on a single cell basis is very important in the field of immunology research where many molecules are secreted from cells in response to external stimuli including immunization. Here we introduce substrates with plasmonic nanoparticles and fluorescence microscopy as promising imaging methods for studies on molecular processes controlling cell behavior, particularly secretion of cytokines. We developed unique composition of silver and silica layers of plasmonic nanostructures which resulted in fluorescence enhancement of more than 200-fold for ensemble of molecules in the immunoassay. Read More

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http://dx.doi.org/10.1007/s12668-013-0076-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3693482PMC

Characterization of Hapten-Protein Conjugates: Antibody Generation and Immunoassay Development for Pesticides Monitoring.

Bionanoscience 2013 Jun;3(2):137-144

Department of Biotechnology, Shoolini Institute of Life Science, Solan, Himachal Pradesh India.

The generation of specific and sensitive antibodies against small molecules is greatly dependent upon the characteristics of the hapten-protein conjugates. In the present study, we report a new fluorescence-based method for the characterization of hapten-protein conjugates. The method is based on an effect promoted by hapten-protein conjugation density upon the fluorescence intensity of the intrinsic tryptophan chromophore molecules of the protein. Read More

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http://link.springer.com/10.1007/s12668-013-0083-8
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http://dx.doi.org/10.1007/s12668-013-0083-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3657092PMC
June 2013
9 Reads

Microwell regulation of pluripotent stem cell self-renewal and differentiation.

Bionanoscience 2012 Dec 11;2(4):266-276. Epub 2012 Sep 11.

Department of Chemical & Biological Engineering, University of Wisconsin - Madison, Madison, WI 53706, USA.

The fates of pluripotent stem cells (PSCs), including survival, self-renewal, and differentiation, are regulated by chemical and mechanical cues presented in the three-dimensional (3D) microenvironment. Most PSC studies have been performed on two-dimensional substrates. However, 3D culture systems have demonstrated the importance of intercellular interactions in regulating PSC self-renewal and differentiation. Read More

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http://dx.doi.org/10.1007/s12668-012-0050-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3589576PMC
December 2012

Applications of Microfluidics in Stem Cell Biology.

Bionanoscience 2012 Dec;2(4):277-286

Department of Physics, Princeton University, Princeton, NJ, 08544, USA.

Stem cell research can significantly benefit from recent advances of microfluidics technology. In a rationally designed microfluidics device, analyses of stem cells can be done in a much deeper and wider way than in a conventional tissue culture dish. Miniaturization makes analyses operated in a high-throughput fashion, while controls of fluids help to reconstruct the physiological environments. Read More

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http://link.springer.com/10.1007/s12668-012-0051-8
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http://dx.doi.org/10.1007/s12668-012-0051-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3546818PMC
December 2012
3 Reads

Towards Measuring Stress with Smartphones and Wearable Devices During Workday and Sleep.

Bionanoscience 2013 ;3:172-183

Wearable Computing Lab, ETH Zurich, Gloriastrasse 35, 8092 Zurich, Switzerland.

Work should be a source of health, pride, and happiness, in the sense of enhancing motivation and strengthening personal development. Healthy and motivated employees perform better and remain loyal to the company for a longer time. But, when the person constantly experiences high workload over a longer period of time and is not able to recover, then work may lead to prolonged negative effects and might cause serious illnesses like chronic stress disease. Read More

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http://dx.doi.org/10.1007/s12668-013-0089-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4269214PMC
January 2013
1 Read

Cyclodextrin-PEI-Tat Polymer as a Vector for Plasmid DNA Delivery to Placenta Mesenchymal Stem Cells.

Bionanoscience 2011 Sep 18;1(3):89-96. Epub 2011 Jun 18.

Department of Chemistry, The University of Hong Kong, Hong Kong SAR, China.

This study aims to modify a cyclodextrin-PEI-based polymer, PEI-β-CyD, with the TAT peptide for plasmid DNA delivery to placenta mesenchymal stem cells (PMSCs). By using the disulfide exchange between the SPDP-activated PEI-β-CyD and TAT peptide, the TAT-PEI-β-CyD polymer was fabricated and the success of this was confirmed by the presence of characteristic peaks for PEI (at δ 2.8-3. Read More

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http://link.springer.com/10.1007/s12668-011-0010-9
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http://dx.doi.org/10.1007/s12668-011-0010-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3460531PMC
September 2011
4 Reads
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