998 results match your criteria Bing du xue bao = Chinese journal of virology[Journal]


Research on the Mechanism of Cross-species Infection and Transmission for Animal-origin Emerging and Re-emerging Zoonosis Has Won Support by the National Key Research and Development Program of China.

Bing Du Xue Bao 2017 Jan;33(1):123-126

Emerging and re-emerging zoonotic diseases caused by pathogens such as Middle East respiratory syndrome coronavirus (MERS-CoV), West Nile virus (WNV) or Chikungunya virus (CHIKV) pose considerable threats to public health worldwide. Research on the mechanism of cross-species infection and transmission for animal-origin emerging and re-emerging zoonosis (2016YFD0500300) has won support by the National Key Research and Development Program of China. Professor Wenjie Tan (National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention) was the primary principal investigator of this research group. Read More

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January 2017

How the Canine Distemper Virus Infects Human Cells at the Molecular Level in Vitro.

Bing Du Xue Bao 2017 Jan;33(1):116-122

Infection by the canine distemper virus (CDV) results in a fulminating infectious disease that causes serious harm to dogs. With breaking 'of the CDV into primates, some researchers wonder if the CDV will cause a serious infection in humans. To better understand the potential of the CDV to infect humans, the molecular characteristics of the CDV, how it infects target cells in the host, the key receptors involved in infection, and infection of human cells in vitro were assessed in this review. Read More

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January 2017

Role of Non-coding RNAs in Interactions between Host and Influenza Virus.

Bing Du Xue Bao 2017 Jan;33(1):108-115

Non-coding RNAs (ncRNAs) are a class of RNAs that have no potential for protein coding. Increasing numbers of studies have provided strong evidence that ncRNAs play important roles in regulation of various biologic processes, including interactions between viruses and the host. Influenza viruses remain a major, threat to human health and animal livestock. Read More

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January 2017
7 Reads

An Overview of Digital PCR.

Bing Du Xue Bao 2017 Jan;33(1):103-107

Digital polymerase chain reaction (dPCR) is a new method for absolute quantification of nucleic acids. The dPCR reaction solution is divided into numerous partitions followed by independent amplification. Target copy number is counted using statistical analyses of positive signals. Read More

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January 2017

Identification of Infectious Salmon Anaemia Virus from Imported Iced Atlantic Salmons.

Bing Du Xue Bao 2017 Jan;33(1):96-102

Infectious Salmon anaemia virus (ISAV) has become a threat to the salmon industry worldwide and has caused considerable economic loss. In the present study, 9 suspect cases of ISAV infection were identified from iced Atlantic salmons imported from Norway in 2014 through Shenzhen port (Shenzhen, China) using methods recommended by the World Organization for Animal Health. However, the results of virus isolation were negative. Read More

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January 2017

Capacity of the Meq-deleted Strain Marek's Virus SC9-1 to Acquire the Meq Gene by Natural Recombination.

Bing Du Xue Bao 2017 Jan;33(1):89-95

We wished to explore the ability of the meq-deleted Marek's disease virus (MDV) vaccine strain SC9-1 to acquire the meq gene from the MDV wild strain Md5 by recombination. Chicken embryo fibroblast cells (CEFs) were co-infected with the SC9-1 vaccine virus and Md5 virus, passaged to third generation, and viral DNA was extracted from a single plaque in the cell culture. Specific pathogen-free chickens pre-immunized with the SC9-1 vaccine virus were infected with the Md5 virus. Read More

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January 2017
1 Read

Detection and Identification of the Bat Circovirus BtCV-DS13.

Bing Du Xue Bao 2017 Jan;33(1):82-88

The bat circovirus has been detected from different bat species and regions after detection in Rousettus leschenaultia. To enrich the epidemiologic data of the bat circovirus, a complete sequence named "BtCV-DS13" was obtained by nested polymerase chain reaction and Genome Walking? based on the intestines of Myotis davidii from Zhoushan Island (Zhejiang Province, China). The complete length of BtCV-DS13 was 1873nt, and it had the typical gene structure of a circovirus according to sequencing analyses. Read More

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January 2017

Study on the National Monthly Reported Severe Cases of Hand-foot-mouth Disease Forecasted by Autoregressive Integrated Moving Average Model.

Bing Du Xue Bao 2017 Jan;33(1):77-81

Autoregressive integrated moving average (ARIMA) model was used to predict the monthly reported severe cases of hand-foot-mouth disease(HFMD) in China to provide a reference for prevention and control of HFMD and the application of ARIMA in of ARIMA in HFMD and other infectious diseases. On the basis of time series supplied by the monthly reported severe cases of the national HFMD from 2010 ~ 2015, ARIMA model was established with the actual cases of HFMD from January to September 2016 as the validated data and with the comparison of ARIMA model based on the data from 2010-2014- The models based on the 2010~2014 and 2010~2015 data of monthly reported severe cases of HFMD in China are ARIMA(1,1,0,) (2, 1,0)(12),ARIMA(0,1,1,) (2,1,0)(12) respectively. The comparison of two models shows that the average of the relative error decreases with the accumulated data and does not do the same with the shorter time of predication. Read More

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January 2017

Dynamic Change in Variation of Rubella Viruses Circulating in Mainland China.

Bing Du Xue Bao 2017 Jan;33(1):67-76

We wished to study the dynamic change in variation of rubella viruses circulating during 1999-2015 in mainland China at the molecular level. Molecular evolution of Chinese rubella viruses collected during 1999 ~ 2015 was analyzed according to a surveillance database of measles/rubella laboratory networks in China. A total of 1737 rubella viruses were obtained from 20 of 31 provinces (except Xinjiang and Tibet) during 1999 ~ 2015. Read More

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January 2017

Genotypes of the Hepatitis C Virus in Infected Patients in Hohhot, China.

Bing Du Xue Bao 2017 Jan;33(1):61-66

We wished to identify the existing HCV genotypes in diagnosed cases of infection by the hepatitis C virus in Hohhot (China) to offer basic data for the treatment and prevention of HCV infection in this area. Outpatients and inpatients were recruited from hospitals in Hohhot from January 2014 to January 2015. In total, 149 patients with HCV infection confirmed by positive anti-HCV and HCV-RNA tests were selected. Read More

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January 2017
2 Reads

Characterization of Glycoproteins and Preparation of HCV Pseudoparticles with High Infectivity of Subtype 3b from Chinese Patients.

Bing Du Xue Bao 2017 Jan;33(1):56-60

We wished to characterize the envelope protein coding gene of the hepatitis-C virus (HCV) from Chinese patients and prepare HCV pseudoparticles (HCVpp) of subtype 3b. Two of the HCV genotype 3b envelope protein coding genes (C27, C30) were cloned from Chinese HCV patients followed by sequencing analyses. Then, two envelope protein expression plasmids were constructed and characterized by western blotting. Read More

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January 2017

A Reverse Transcription Loop-Mediated Isothermal Amplification Assay for Detection of HCV Genotypes 1b and 2a in China.

Bing Du Xue Bao 2017 Jan;33(1):49-55

Reverse transcription loop-mediated isothermal amplification (RT-LAMP) was used to detect hepatitis C virus (HCV) lb and 2a (the major genotypes in China). First, clinical samples were collected, and the genotypes and viral load determined. Second, RT-LAMP primers of HCV lb and 2a were designed according to the 5' untranslated region of the HCV. Read More

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January 2017

Analysis on Genotype Distribution and Mutation of Major Hydroponic Region of Hepatitis B Virus in Henan Province.

Bing Du Xue Bao 2017 Jan;33(1):44-48

To understand the genotype and the mutation of amino acid (aa) in the major hydrophilic region (MHR) of hepatitis B virus(HBV). The serum samples were collected from the surveillance of HBV among population in Henan in 2012. The S gene of HBV was amplified and sequenced. Read More

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January 2017
2 Reads

Analyses of the Genetic Diversities and Mutations of the Hepatitis B Virus Genome BCP/Pre C Region.

Bing Du Xue Bao 2017 Jan;33(1):36-43

We wished to study the genetic diversities and mutations of the basic core promoter (BCP) and pre-C region of the hepatitis B virus (HBV) in liver-cancer tissues. One hundred and ninety-two tissue samples were collected from patients suffering from hepatocellular carcinoma (HCC) and HBV infection in 2015 in Xijing Hospital. Twenty-one cases were selected, of which direct sequencing of the polymerase chain reaction (PCR)-amplified products of BCP/pre-C region was unsuccessful. Read More

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January 2017

KNK437 Inhibits Replication and Transcription of the Hepatitis B Virus.

Bing Du Xue Bao 2017 Jan;33(1):24-35

During replication of the hepatitis B virus (HBV) in liver cells, the reverse transcription of pre-genomic RNA (pgRNA) is initiated by protein priming at an RNA packaging signal ε located near the 5' end of pgRNA. Heat-shock proteins (Hsps) such as Hsc70, Hsp40, and Hsp90 have been reported to be involved in the reconstitution of HBV polymerase (P protein) and E. The P - E complex initiates the reverse transcription and assembly of nucleocapsids. Read More

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January 2017
13 Reads

Method for Sequencing the Whole Genome of the Highly Pathogenic Avian Influenza A(H5N6) Virus.

Bing Du Xue Bao 2017 Jan;33(1):19-23

We wished to develop a simple method to amplify and sequence the whole genome of the highly pathogenic avian influenza A ( H5N6 ) virus. Hemagglutinin ( HA ) gene sequences of A ( H5), neuraminidase (NA) gene sequences of A (N6), as well as the internal gene sequences of subtype A (H5N1) and A (H9N2) influenza viruses of the-previous 5 years were downloaded from GenBank and GISAID, and individual genes were aligned. Thirty-two primer pairs targeted to conserved regions of these gene sequences were designed and validated. Read More

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January 2017

Effect of Oseltamivir on the Hemagglutination Test and Hemagglutination Inhibition Test of the Influenza A(H3N2) Virus in China.

Bing Du Xue Bao 2017 Jan;33(1):13-18

We compared the effect of oseltamivir on the hemagglutination (HA) test and hemagglutinin inhibition (HI) test of the influenza A(H3N2) virus in China to obtain the "true" HA titer and antigenic variation. A total of 395 influenza H3N2 strains isolated in mainland China from October 2014 to May 2015 were analyzed with HA and HI tests, with or without oseltamivir.. Read More

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January 2017

Comparison of Pathogenicity between PR8F and Different Mutants on the NS1 Locus in Mice.

Bing Du Xue Bao 2017 Jan;33(1):6-12

The aim of this study was to explore the influence of mutation of different non-structural (NS) 1 amino-acid residues on the pathogenicity of influenza viruses and the function of NS1 virulence-related sites on the pathogenesis of influenza viruses. We analyzed segments of the NS1 protein gene and key sites related to virulence of influenza viruses based on a literature review. Fragments of the NS1 gene were cloned from the HIN1 subtype PR8F (non-mutated) and preserved by our research team with encoding sequence site-specific mutagenesis at aa42, aa8l, and aa149. Read More

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January 2017
7 Reads

Droplet Digital Polymerase Chain Reaction Method for Absolute Quantification of Influenza A Viruses.

Bing Du Xue Bao 2017 Jan;33(1):1-5

Digital polymerase chain reaction (dPCR) is a new method for absolute quantification. However, an absolute quantification method for influenza A viruses based on droplet digital polymerase chain reaction (dPCR) has not been established. In this study, we found that: (i) the annealing temperature of dPCR was optimized at 64, 4°C; (ii) the detection range of dPCR was 37. Read More

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January 2017

[Immune Effects and Mechanisms of HIV-specific Antibodies Against Viral Infection].

Bing Du Xue Bao 2016 11;32(6):830-8

Broadly neutralizing antibodies (bNAbs) have demonstrated a protective role from experimental challenge in non-human primates and humanized mouse models. Recently, bNAbs 3BNC117 and VRC01were assessed in a phase-I clinical trial, and were shown to lower plasma viremia in human immunodeficiency virus(HIV)-1-infected individuals not receiving antiretroviral therapy. However, induction of these types of antibodies by vaccination iS extremely difficult. Read More

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November 2016

[Current Status of Middle East Respiratory Syndrome Vaccines].

Bing Du Xue Bao 2016 11;32(6):825-9

Middle East respiratory syndrome is a kind of respiratory disease Caused by Middle East respiratory syndrome Coronavirus. Now the disease has spread to 27 countries around the world, more than 1806 people infected and more than 600 people were killed, and four countries have reported cases of camel infection MERS. Prevention of the disease are very important measures to research and development related to the vaccine. Read More

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November 2016
1 Read

[Research Advances in VP16 of the Herpes Virus].

Bing Du Xue Bao 2016 11;32(6):817-24

Viral protein(VP)16is an important tegument protein of the herpes virus. It is involved in early transcription activation of viral genes as well as virion assembly and release in host cells.VP16 of some herpes viruses have deubiquitinating protease activity and can help the virus counteract the host immune response. Read More

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November 2016
1 Read

[Progress in the Development of Animal Models Supporting Human Adenovirus Infection].

Bing Du Xue Bao 2016 11;32(6):810-6

Human adenoviruses cause respiratory diseases, conjunctivitis, gastroenteritis and even severe pneumonia. Specific antiviral drugs and vaccines are still unavailable. Animal models that support adenovirus infection and pathogenesis are critical for the study of these viruses and the development of prophylactic and therapeutic strategies. Read More

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November 2016

[Research Progress in Virus Infection Altering Cellular Glucose Metabolism].

Bing Du Xue Bao 2016 11;32(6):800-9

Viruses "hijack" cellular metabolism to complete their proliferation. Glucose is an important source of energy and carbon in the synthesis of precursor molecules in host cells, and its metabolism is regulated dramatically during virus infection. Here, we reviewed the mechanism of virus infection that alters glucose transport, expression of glucose metabolism-related genes (glycolysis, pentose phosphate pathway, gluconeogenesis) in cells, as well as islet cells and insulin receptors. Read More

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November 2016
18 Reads

[Advances in the Role of Stem Memory T Cells in HIV-1 Infection].

Bing Du Xue Bao 2016 11;32(6):796-9

Stem memory T cells represent the earliest and long-lasting memory T cells. They display stem cell-like properties of self-renewal and multipotency. Tscm cells have shown their antitumor effect; the role of Tscm cells has sparked interest in anti-HIV fields recently. Read More

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November 2016

[Preparation and Preliminary Application of a Virus Library for the Neutralizing Activity of a Monoclonal Antibody Against the Rabies Virus].

Bing Du Xue Bao 2016 11;32(6):790-5

To establish a method for measurement of the neutralizing activity of a monoclonal antibody against the rabies virus. Twenty-four rabies street virus-positive samples were isolated by the mouse inoculation test. Isolated rabies street viruses were cultured and the virus titer tested in N2A cells. Read More

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November 2016
1 Read

[Isolation and Identification of the Nam Dinh Virus from Mosquitoes on the China-Laos-Myanmar Border].

Bing Du Xue Bao 2016 11;32(6):782-9

Three strains of an insect nidovirus, the Nam Dinh virus (NDiV), isolated in Yunnan Province, China, have been identified. Aedes albopictus C6/36 cells were used to isolate NDiV from mosquitoes collected in Yunnan Province in 2012.Culture supernatants with a positive cytopathic effect were harvested for virus identification by sequence-independent single primer amplification. Read More

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November 2016
2 Reads

[Influence of Synonymous Codon Bias on the RNA Secondary Structure in Influenza-A Viruses].

Bing Du Xue Bao 2016 11;32(6):773-81

Influenza-A viruses were selected as samples. Then, the relationship between synonymous codon bias and mRNA secondary structures was analyzed. Our data will provide a theoretical basis for further research on the synonymous codon and RNA properties of the influenza-A virus. Read More

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November 2016
1 Read

[The Effect of Influenza Type B Inter-lineage Reassortment on the Growth Characteristics of Influenza].

Bing Du Xue Bao 2016 11;32(6):768-72

This study aims to research the effect of inter-lineage reassortment of type B influenza virus on its biological characteristics. The representative strains isolated in 2013~2015were selected, which included wild type viruses and inter-lineage reassortment viruses. We tested the growth curve of each virus based on the value of TCID50 at different time point. Read More

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November 2016
1 Read

[Whole Genome Sequencing and Phylogenetic Analyses of Sub-genotype A1 of the Human Metapneumovirus Detected in an Infant with Pneumonia].

Bing Du Xue Bao 2016 11;32(6):758-67

The human metapneumovirus (HMPV) is an important pathogen in respiratory-tract infections in children. We undertook genomic sequence analyses and described the genetic characteristics of an uncommon sub-genotype, the HMPV A1 strain, and provide useful data for further studies. The HMPV A1(BJ-1610)strain was identified from a nasopharyngeal aspirate collected from a 3-month-old female with bronchopneumonia. Read More

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November 2016
6 Reads

[Molecular Epidemiology of Astrovirus Infection Among Hospitalized Infants in Kunming City, China, 2013~2014].

Bing Du Xue Bao 2016 11;32(6):752-7

We investigated the infection and molecular-epidemiologic characteristics of human astrovirus (HAstV) of hospitalized infants in Kunming City from the year 2013 to 2014.Infection and genotype of HAstV of 63 samples of diarrheal feces and 42 controls were analyzed by reverse transcription-polymerase chain reaction(RT-PCR).The complete genome sequence of a HAstV strain was amplified and sequenced. Read More

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November 2016

[Effect of PLSCR1 on the Antiviral Activity of IFN against HBV in HepG2 Cells].

Bing Du Xue Bao 2016 11;32(6):747-51

To study the effect of interferon(IFN)against hepatitis B virus(HBV)by silencing phospholipid scramblase (PLSCR)1in HepG2 cells. siRNA specific for PLSCR1 was designed and transfected in HepG2 cells. The inhibitory effect of siRNA was determined using semi-quantitative polymerase chain reaction(PCR)and western blotting 48hpost-transfection. Read More

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November 2016
6 Reads

[Development and Application of Reverse Transcription Loop-mediated Isothermal Amplification Method for Detection of Porcine Rotavirus of Swine].

Bing Du Xue Bao 2016 11;32(6):740-6

We wished to establish a method for rapid and sensitive detection of reverse transcription loop-mediated isothermal amplification(RT-LAMP)for the rapid and sensitive detection of porcine rotavirus (PoRV). According to the published PoRV VP7 sequences in GenBank,6specific primers were designed. According to the concentrations of foward and reverse primers, Bst DNA polymerase, Mg(2+), and dNTP, reaction conditions were optimized. Read More

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November 2016

[Canine Parvovirusin Diarrheal Dogs and Analyses of the Full-Length VP2 Gene of Dogs].

Bing Du Xue Bao 2016 11;32(6):733-9

We investigated infection by canine parvovirus and genetic variation of the VP2 gene. We collected feces samples of 50 diarrheal dogs in Sichuan Province, China. Analyses polymerase chain reaction (PCRs), agarose gel electrophoresis, and amplification of the complete sequence of canine parvovirus were done. Read More

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November 2016
4 Reads

[Molecular Epidemiology of Echovirus 30 in Longyan City, Fujian, China, 2011~2014].

Bing Du Xue Bao 2016 11;32(6):727-32

We studied the molecular epidemiology of echovirus 30 in sporadic cases of viral encephalitis in Longyan City, Fujian, China, from 2011 to 2014.Specimens of cerebrospinal fluid from patients diagnosed with viral encephalitis or infection of the central nervous system were collected. Viruses were isolated by cell culture. Read More

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November 2016
3 Reads

[Pathogenic Analyses of an Outbreak of Viral Encephalitis Caused by ECHO30 in Guazhou of Gansu Province, China].

Bing Du Xue Bao 2016 11;32(6):721-6

We used molecular-biology methods to identify the pathogens that caused an outbreak of viral encephalitis in Guazhou (Gansu province, China)during June-August 2015.We also undertook molecular characterizations of these pathogens. A total of 132samples(14cerebrospinal fluid(CSF)samples;25throat swabs;66serum samples;27fecal samples)were collected from 74 patients during the outbreak of viral encephalitis. Read More

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November 2016
9 Reads

[Epidemiology Characteristics and Pathogen Surveillance of Hand, Foot and Mouth Disease in Guangdong Province, China, 2008~2015].

Bing Du Xue Bao 2016 11;32(6):713-20

To understand the epidemiological etiology characteristics of hand, foot and mouth disease(HFMD)in Guangdong province, and to explore the risk change trend of the whole province. By using the descriptive epidemiological methods, the whole province’s incidence trend, population distribution and pathogenic form of HFMD were analyzed with the HFMD surveillance data,population data and geographic information of Guangdong province from 2008 to 2015.The analysis results show: A total of 2,133,722 cases of HFMD, including 5,066 severe cases and 259 death cases were reported in Guangdong province from 2008 to 2015. Read More

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November 2016
1 Read

[The Epidemiology and Etiology Characteristics of Hand-foot-mouth Disease in Chongqing, China,2014~2015].

Bing Du Xue Bao 2016 11;32(6):707-12

In this study, the epidemiology of Hand-foot-mouth disease(HFMD)composition of enterovirus (EV) pathogen and VP1 coding gene of Enterovirus A71(EV-A71)were analyzed in Chongqing from 2014 to 2015,to provide a scientific basis for strategies of prevention and control of HFMD in Chongqing. It is reported that there were a total of 100,176 cases of HFMD, of which 284 cases of severe,37 cases of death in Chongqing.39counties(autonomous counties)of Chongqing have reported cases, and the urbans reported incidence rate(298. Read More

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November 2016
3 Reads

[Genetic Characterization of Coxsackievirus A6 Strains Isolated in Ningxia Region, China, 2013~2015].

Bing Du Xue Bao 2016 11;32(6):702-6

To study the genetic characteristics of coxsackievirus (CV)-A6 strains isolated from hand, food and mouth(HFMD)cases in Ningxia Province, China, in 2013~2015.A total of 998 specimens identified as non-EV71,non-CVA16 enteroviruses by real-time polymerase chain reaction were collected in 2013~2015and cultured using RD cells. The viral protein(VP)1gene of isolated strains was amplified by reverse transcription-polymerase chain reaction with degenerate primers and sequenced. Read More

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November 2016
1 Read

[Expression of Type-Ι Interferon Production Pathway-Related Genes Induced by Infection Due to Enterovirus 71 or Coxsackievirus A16 in Normal Human Airway Epithelial Cells].

Bing Du Xue Bao 2016 11;32(6):694-701

Hand, foot, and mouth disease(HFMD)is caused by mainly enterovirus 71(EV-A71)and coxsackievirus A16(CV-A16),and is a serious healthcare problem worldwide.EV-A71 infection is thought to progress readily to serious complications whereas CV-A16 infection, in general, results in mild symptoms and presents repeatedly. However, the underlying mechanisms leading to these differences are not known. Read More

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November 2016
7 Reads

[Genetic Characteristics of the VP1 Region of the Coxsackie A16 Virus from Jiangsu Province, China, in 2015].

Bing Du Xue Bao 2016 11;32(6):689-93

The aim of this study was to analyze the genetic characteristics of the VP1 gene of coxsackievirus A16(CA16)strains isolated from Jiangsu Province, China, in 2015.The VP1 regions of 20CA16 virus strains from Jiangsu Province in 2015 were amplified, and the amplification products were sequenced. Mega 6. Read More

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November 2016
3 Reads

[Phylogenetic Analysis of Complete VP1 Sequence of Coxsackievirus B1 in Shandong Province, China ].

Bing Du Xue Bao 2016 11;32(6):683-8

To investigate the molecular epidemiological characterization of coxsackieivirus B1(CV-B1),we performed VP1 sequencing on all isolates from acute flaccid paralysis (AFP) surveillance, environmental surveillance and viral meningitis specimens from 1994 to 2015.A total of 53CV-B1 strains were isolated, among which 41 strains,4strains,and 8strains were obtained from AFP surveillance, environmental surveillance and meningitis specimens, respectively. Phylogenetic analysis based on VP1 entire coding region revealed that Shandong CV-B1 strains were segregated into a major cluster alongside with other domestic strains, with no foreign strains existing in this cluster. Read More

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November 2016
3 Reads

[Establishment of a Model of Infection by Enterovirus 71 in ICR Mice].

Bing Du Xue Bao 2016 11;32(6):671-82

We aimed to study infections in neonatal ICR mice of different ages infected with Enterovirus 71(EV-A71)through three routes of infection, and to explore the dynamic distribution and infection mechanism of EV-A71 in vivo.Three-,5-and 9-day-old neonatal ICR mice were infected with an EV-A71 strain isolated from a child with severe hand, foot and mouth disease through intramuscular(IM), intraperitoneal (IP)and intracerebral (IC)injection, respectively. Consequently, blood, brain, hind-limb muscle, heart, and intestines of mice were collected at regular intervals. Read More

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November 2016
8 Reads

[Advances in Application of Rabies Immunoglobulin].

Bing Du Xue Bao 2016 Sep;32(5):666-70

China has the second highest number of rabies cases worldwide. There are millions of category III rabies exposure cases in China every year, which are treated with rabies immunoglobulin(RIG)and the rabies vaccine. The price of RIG is relatively expensive, and the application of RIG is relatively complicated. Read More

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September 2016

[Analysis of Common Issues and Research Progress in Loop Mediated Isothermal Amplification].

Bing Du Xue Bao 2016 09;32(5):659-65

Loop mediated isothermal amplification(LAMP)technology is a newly developed isothermal amplification technology for in vitro detection of nucleic acids. Although the LAMP assay is rapid, specific, sensitive, simple and has been widely applied for rapid detection of nucleic acids, it continues to improve and develop. In this paper, we summarize approaches to addressing amplification product contamination, primer design to avoid false positives, and the development of related techniques based on LAMP technology. Read More

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September 2016
1 Read

[Recent Advances in Hepatitis B Virus Antivirals].

Bing Du Xue Bao 2016 09;32(5):650-8

The ultimate goal of chronic hepatitis B virus(HBV)therapy is full eradication of the virus from the liver. However, this is rarely achieved with the clinically available first-line agents (entecavir and tenofovir disoproxil fumarate) due to the inability to eliminate covalently closed circular DNA(cccDNA), which persists in the nucleus of infected hepatocyte cells,and failure of the host to induce an adequate specific immune response to control the infection. Currently, the clinical treatment for chronic HBV infection mainly includes nucleos(t)ide analogues (NAs), non-NAs and immune modulatory agents; however, each agent has individual advantages and drawbacks. Read More

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September 2016
8 Reads

[Varieties and Mechanisms of Synergistic Factors Enhancing Baculovirus Infectivity].

Bing Du Xue Bao 2016 09;32(5):640-9

Baculoviruses are a diverse group of viruses with double-stranded circular DNA genomes. They have certain advantageous properties for biotechnology applications, including high host specificity and environmental friendliness.Baculoviruses could play more important roles in sustainable agriculture as potential microbial insecticides. Read More

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September 2016
1 Read

[Recent Progress and Practical Prospects for the HIV-1 Capsid Structure].

Bing Du Xue Bao 2016 09;32(5):634-9

The human immunodeficiency virus type 1(HIV-1)has spread globally and often exhibits antiviral resistance. Therefore, there is an ongoing need for the development of novel, highly efficient antiretroviral drugs with low toxicity. The capsid protein(CA),which is composed of an N-terminal domain(NTD)and C-terminal domain(CTD),plays an important role in the process of HIV-1assembly and maturation. Read More

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September 2016
6 Reads

[Advances in Avian Antiviral Innate Immune Effectors].

Bing Du Xue Bao 2016 09;32(5):627-33

As direct antiviral effector proteins, the innate antiviral proteins induced by pathogens and interferon can restrict intracellular viral infection at an early stage, establishing the state of the host antiviral immune response. Mx, PKR, OAS, IFITM, ZAP, and other proteins have all been shown to play important roles in the antiviral response. The host innate immune factors Mx and IFITM are both key antiviral effectors against influenza, while the antiviral mechanism of viperin remains to be completely elucidated. Read More

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September 2016
2 Reads

[Isolation and Identification of a Novel Cypovirus from Daphnis nerii].

Bing Du Xue Bao 2016 09;32(5):619-26

In order to develop a novel effective biological insecticide for controlling oleander hawk moth, a new pathogen was isolated from naturally diseased Daphnis nerii. Based on scanning electron microscopy, full-length amplification of cDNAs (FLAC), and phylogenetic analysis of genome segments 2and 10,the virus was identified as a new type of cypovirus (Da phnis nerii cypovirus [DnCPV]). Electrophoresis analysis showed that DnCPV had a genome comprising 10double-stranded RNA (dsRNA) segments, ranging from 892 to 4160bp. Read More

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September 2016
22 Reads