137,061 results match your criteria Bile Duct Strictures
Liver Int 2018 Dec 14. Epub 2018 Dec 14.
Department of Pathology, Institut Jules Bordet, Université Libre de Bruxelles, Brussels, Belgium.
Laboratory tests in an asymptomatic 70-year-old Caucasian man revealed biological cholestasis with an increased level of γ-GT. Abdominal tomography showed a mass in the left hepatic lobe, nearly four centimeters in diameter and associated with a dilated left bile duct. PET imaging revealed high glucose uptake. Read More
Asian J Endosc Surg 2018 Dec 13. Epub 2018 Dec 13.
Department of Surgery, The Jikei University School of Medicine, Tokyo, Japan.
Introduction: Recently, single-incision laparoscopic cholecystectomy has been accepted as an alternative to conventional laparoscopic cholecystectomy. The aim of this study was to retrospectively evaluate the safety and feasibility of unique gallbladder retraction methods using an ENDOLOOP® (Ethicon, Tokyo, Japan) and Lapaherclosure™ (Hakko Medical, Tokyo, Japan).
Materials And Surgical Technique: From May 2013 to April 2015, 77 patients underwent single-incision laparoscopic cholecystectomy with this retraction technique. Read More
Asian J Endosc Surg 2018 Dec 13. Epub 2018 Dec 13.
Division of Surgery, Gastroenterological Center, Medico Shunju Shiroyama Hospital, Osaka, Japan.
Introduction: A 50-year-old Japanese man presented with obstructive jaundice. We performed endoscopic retrograde biliary drainage before biliary decompression. CT showed a thickened gallbladder wall with low-density areas and a 35-mm gallstone; the stone was impacted in the gallbladder neck and cystic duct. Read More
J Cell Physiol 2018 Dec 12. Epub 2018 Dec 12.
Key Laboratory of Nanobiological Technology of Chinese Ministry of Health, Xiangya Hospital, Central South University, Changsha, Hunan, People's Republic of China.
Objective: Glycochenodeoxycholate acid (GCDA) is a toxic component in bile salts. It plays an important role in the development and progression of liver cancer. In this study, we investigated the underlying mechanism of GCDA in hepatocarcinogenesis and chemotherapy resistance. Read More
J Forensic Sci 2018 Dec 12. Epub 2018 Dec 12.
Forensic Medicine Department, University Hospital of Lyon, 5 place d'Arsonval, Lyon, France.
Fatalities implicating psychedelic mushrooms are not a common clinical situation in everyday forensic medicine. Despite classification as an illegal drug in many countries, psilocybin mushrooms have the reputation of being safe. We report the case of a young man who jumped from a second story balcony under the influence of psilocybin mushrooms. Read More
Ugeskr Laeger 2018 Dec;180(50)
As cases of gallbladder-related adverse events have been reported in patients treated with glucagon-like peptide (GLP)-1 receptor agonists and GLP-2 receptor agonists, studies have investigated the effects of these hormones on the enterohepatic circulation of bile acids and gallbladder motility. Results suggest, that bile acids and GLP-2 counteract postprandial cholecystokinin-mediated gallbladder contraction, while GLP-1 may delay gallbladder refilling. This provides important information on the effect and safety of drugs based on GLP-1 and GLP-2, respectively. Read More
J Health Pollut 2017 Mar 29;7(13):2-10. Epub 2017 Mar 29.
Department of Chemical Engineering, Obafemi Awolowo University, Ile-Ife, Osun State, Nigeria.
Background: Nigeria has a growing spray painting industry, however, the burden of occupational health problems related to organic solvent exposure among spray painters in Nigeria is under-studied.
Objectives: This study aimed to assess workshop characteristics and ambient concentration of total volatile organic compounds (VOCs) in spray painting workshops and to compare lung function status and total serum bile acid levels of spray painters and controls.
Methods: A cross-sectional study design was employed to survey 120 spray painters and 120 controls (electronic technicians). Read More
Ann Med Surg (Lond) 2019 Jan 30;37:7-10. Epub 2018 Nov 30.
Baghdad Medical City, Iraq.
Background: Gall stones are the most common biliary pathology. Subclinical hypothyroidism is not a common problem in the population with thyroid disease, several explanations for a possible relation between hypothyroidism and lipid metabolism, gall stone formation proved that prevalence of gall stones is increased in patients with hypothyroidism disease.
Objective: To ﬁnd the prevalence and correlation between the subclinical hypothyroidism and gall stone disease. Read More
Oncotarget 2018 Nov 16;9(90):36220-36237. Epub 2018 Nov 16.
Department of Internal Medicine I, University of Bonn, Bonn, Germany.
Background And Aims: Angiogenesis is critically involved in the development of liver fibrosis, portal hypertension (PHT) and hepatocellular carcinoma (HCC). Regorafenib is a novel second-line therapy for HCC, but might also be beneficial in fibrosis and PHT even in absence of HCC. This study investigated the effects of regorafenib in experimental models without HCC. Read More
Exp Ther Med 2018 Dec 5;16(6):5224-5226. Epub 2018 Oct 5.
Department of Gastroenterological Surgery I, Hokkaido University Graduate School of Medicine, Sapporo, Hokkaido 060-8638, Japan.
It is often difficult to correctly diagnose patients who present with dilation of the bile duct. Cholangiocarcinoma, primary sclerosing cholangitis (PSC) and immunoglobulin (Ig)G4-related sclerosing cholangitis must be considered as potential diagnoses for these cases. The current study presents a 73-year-old female patient who presented with a high fever and abdominal pain. Read More
J Korean Med Sci 2018 Dec 22;33(51):e324. Epub 2018 Nov 22.
Department of Pediatrics, Asan Medical Center Children's Hospital, University of Ulsan College of Medicine, Seoul, Korea.
Oxysterol 7α-hydroxylase deficiency is a very rare liver disease categorized as inborn errors of bile acid synthesis, caused by mutations. As it may cause rapid progression to end-stage liver disease even in early infancy, a high index of suspicion is required to prevent fatal outcomes. We describe the case of a 3-month-old boy with progressive cholestatic hepatitis and severe hepatic fibrosis. Read More
Molecules 2018 Dec 7;23(12). Epub 2018 Dec 7.
Key Laboratory of Shaanxi Administration of Traditional Chinese Medicine for TCM Compatibility, Shaanxi University of Chinese Medicine, Xi'an 712046, China.
stir-fried with vinegar (V-kansui) has promising biological activities toward treating malignant ascites with reduced toxicity compared to crude kansui. But the mechanism concerning promoting the excretion of ascites has not been systematically studied. The purpose of this paper was to investigate the possible mechanism of V-kansui in treating malignant ascites, including metabolic pathways and molecular mechanism using an integrated serum and urine metabolomics coupled with network pharmacology. Read More
Medicine (Baltimore) 2018 Dec;97(49):e13466
Department of Diagnostic Pathology, Jichi Medical University Hospital.
Little is known concerning the prognostic significance of the degree of lymphatic vessel invasion in pancreatic head cancer. To address this gap in knowledge, we retrospectively examined 60 patients with locally advanced, surgically resectable pancreatic head cancer who underwent pancreaticoduodenectomy and lymph node (LN) dissection.All cases were histopathologically diagnosed as ductal adenocarcinoma, stage II (25 pT3N0 cases, 35 pT3N1 cases). Read More
Medicine (Baltimore) 2018 Dec;97(49):e13372
Department of Oncology, First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan Province, China.
Rationale: Intrahepatic cholangiocarcinoma (ICC) originates from the secondary branch of the bile duct and the intrahepatic bile duct epithelial cells, and is a rare pathological type of primary liver cancer. Recently, apatinib has been successfully used for a variety of malignancies.
Patient Concerns: A 23-year-old female was noted with intermittent right upper abdominal distension, abdominal pain, and vomiting after eating for more than 1 month. Read More
Medicine (Baltimore) 2018 Dec;97(49):e13353
Department of Clinical Biochemistry, Jagiellonian University Medical College, Krakow, Poland.
Rationale: X-linked adrenoleukodystrophy (X-ALD) is a rare disorder caused by mutations in the ABCD1 gene, coding for peroxisomal membrane transporter adrenoleukodystrophy (ALD) protein. The disease is characterized by accumulation of very long chain fatty acids (VLCFAs) in tissues. Adult adrenomyeloneuropathy (AMN) and the cerebral inflammatory form of ALD are the main phenotypes presenting various symptoms. Read More
Mol Cell Endocrinol 2018 Dec 10. Epub 2018 Dec 10.
Department of Internal Medicine III, Giessen University Hospital, Germany.
Background And Aim: Bile acids (BA) are increasingly recognized as pleiotropic and hormone-like signaling molecules with metabolic and endocrine functions. However, the role of BA in white adipocyte physiology remains somewhat obscure. It was the aim to investigate the BA receptors (FXR, TGR5) and FGFR1 (Fibroblast growth factor receptor 1) as well as Bsep (bile salt export pump) in white adipocytes and in murine and human adipose tissue (AT) and to investigate effects of different BA species in adipocyte physiology. Read More
Steroids 2018 Dec 10. Epub 2018 Dec 10.
Department of Biotechnology, Deen Bandhu Chhotu Ram University of Science and Technology, Murthal, Sonipat, India.
Cholesterol is the most important sterol synthesized by most of the human cells majorly in the liver. It is a necessary constituent of cell membranes, it acts as a precursor for the synthesis of steroid hormones, vitamin D, and bile acids. Cholesterol is transported in plasma primarily in the form of low-density lipoproteins (LDL), the principal route for its removal from tissues to the liver is in high-density lipoproteins (HDL), followed by excretion in the bile. Read More
Anal Biochem 2018 Dec 10. Epub 2018 Dec 10.
China National Tobacco Quality Supervision and Test Center, Zhengzhou, 450001, China. Electronic address:
Profiling of carboxyl-containing metabolites in smokers and non-smokers provides insight into the smoking-related biological events and causal relationships between exposure and adverse events. However, more comprehensive analysis of carboxyl-containing metabolites in bio-matrices with high sensitivity and accuracy is challenging. In this work, stable isotope labeling in combination with liquid chromatography-tandem mass spectrometry was used for untargeted profiling and relative quantification of carboxyl-containing metabolites in plasma of smokers and non-smokers. Read More
J Invest Surg 2018 Dec 13:1-9. Epub 2018 Dec 13.
a Hepatobiliary Surgery Institute, Southwest Hospital , Army Medical University , Chongqing , China.
Background: Stone recurrence is a major problem limiting the effects of surgical treatment for hepatolithiasis. It was showed that hyperplasia of perihilar liver may compress the hepatic portal and cause deficient bile flow because of compressed hilar bile duct, thereby leading to the formation of bile stasis and precipitating stone recurrence. This retrospective study aimed to evaluate the efficiency of perihilar hepatectomy for hepatolithiasis with compressed hilar bile duct induced by perihilar hyperplasia of liver. Read More
Oncol Rep 2018 Oct 29. Epub 2018 Oct 29.
Department of Biobank, The First Affiliated Hospital of Xiamen University, Xiamen, Fujian 361003, P.R. China.
Ursodeoxycholic acid (UDCA) is a type of hydrophilic bile acid extracted from animal bile with a wide range of biological functions. The present results demonstrated that UDCA could effectively inhibit the proliferation of two human melanoma cell line (M14 and A375) with time‑ and concentration‑dependence. Following exposure to various concentrations of UDCA, M14 cells exhibited typical morphological changes and weaker ability of colony forming. Read More
Turk J Gastroenterol 2018 Dec 12. Epub 2018 Dec 12.
Clinic of General Surgery, İzmir Katip Çelebi University Atatürk Training and Research Hospital, İzmir, Turkey.
BMC Cancer 2018 Dec 12;18(1):1244. Epub 2018 Dec 12.
Departments of Surgery, College of Medicine, Hanyang University222 Wangsimni-ro, Seongdong-gu, Seoul, 04763, South Korea.
Background: SSBP2, single-stranded DNA binding protein 2, is a subunit of the ssDNA-binding complex that is involved in the maintenance of genome stability. The majority of previous studies have suggested a tumor-suppressive role of SSBP2, which is silenced by promoter hypermethylation in several human malignancies, such as hematologic malignancies, prostate cancer, esophageal squamous cell carcinoma, ovarian cancer, and gallbladder cancer. However, an oncogenic role of SSBP2 has been suggested in glioblastoma patients. Read More
Front Pediatr 2018 20;6:354. Epub 2018 Nov 20.
Department of Pediatrics, The First Affiliated Hospital of Jinan University, Guangzhou, China.
Sodium taurocholate cotransporting polypeptide (NTCP) is a carrier protein encoded by the human gene, acting as the principal transporter of conjugated bile salts from the plasma into hepatocytes. Although NTCP was cloned as early as in 1994 and its function has been studied extensively, clinical description of NTCP deficiency remains rather limited thus far. The patients in this paper were 2 female monozygotic twins, who were referred to our hospital at the age 2 years with the complaint of persistently-raised total bile acids (TBA) for 21 months. Read More
Front Microbiol 2018 22;9:2849. Epub 2018 Nov 22.
INSERM U1149, Centre de Recherche sur l'Inflammation, Faculté de Médecine Paris Diderot, Université Paris Diderot, Paris, France.
Bile acids (BA) influence germination and growth of . Ursodeoxycholic acid (UDCA), a BA minor in human, used for cholestatic liver diseases, inhibits germination and growth of , but was never tested with an infectious challenge versus control. We hypothesized that UDCA could prevent CDI. Read More
Pharm Res 2018 Dec 6;36(1):23. Epub 2018 Dec 6.
Technical Research Center, KOBELCO Eco-Solutions Co., Ltd., 1-1-4 Murotani, Kobe, Hyogo, 651-2241, Japan.
Purpose: Straight-chain polysaccharides have a greater potential of selectively adsorbing hydrophobic bile salts than resin-based bile salt sequesters because of ionic and hydrophobic interactions; hence, they may possess antidiabetic activity. The feasibility of using cationic polysaccharides made from euglenoid β-1,3-glucan (referred to as paramylon) as potential antidiabetic agents was examined by using in vitro and animal experiments.
Methods: Cationic straight-chain polysaccharides were synthesized from euglenoid polysaccharide and glycidyltrimethylammonium chloride. Read More
Nat Med 2018 Dec;24(12):1789-1790
Center for Pharmacogenetics and Department of Pharmaceutical Sciences, University of Pittsburgh, Pittsburgh, PA, USA.
BMJ 2018 Dec 6;363:k5155. Epub 2018 Dec 6.
Addenbrooke's Hospital, Cambridge, UK.
Mayo Clin Proc 2018 Dec;93(12):1858-1872
Clinical Enteric Neuroscience Translational and Epidemiological Research (C.E.N.T.E.R.) Program and Division of Gastroenterology and Hepatology, Mayo Clinic, Rochester, MN. Electronic address:
Irritable bowel syndrome (IBS) is associated with diverse pathophysiologic mechanisms. These mechanisms include increased abnormal colonic motility or transit, intestinal or colorectal sensation, increased colonic bile acid concentration, and superficial colonic mucosal inflammation, as well as epithelial barrier dysfunction, neurohormonal up-regulation, and activation of secretory processes in the epithelial layer. Novel approaches to treatment include lifestyle modification, changes in diet, probiotics, and pharmacotherapy directed to the motility, sensation, and intraluminal milieu of patients with IBS. Read More
J Clin Endocrinol Metab 2018 Dec 12. Epub 2018 Dec 12.
Department of Internal Medicine, Division of Endocrinology, Diabetology, Vascular Disease, Nephrology and Clinical Chemistry, University of Tübingen, Germany.
Context: The effect of a lifestyle intervention to reduce liver fat content in nonalcoholic fatty liver disease in humans is influenced by genetics. We hypothesized that the functionally active amino acid exchange in humans Gly388Arg (mouse homologue: Gly385Arg) in the fibroblast growth factor receptor 4 (FGFR4), which regulates bile acid, lipid and glucose metabolism, may determine the dynamics of hepatic lipid accumulation and insulin sensitivity in humans. Mechanisms of this substitution were studied in mice under normal chow and high-fat diet. Read More
Ear Nose Throat J 2018 Dec;97(12):E21-E27
MediENT Ear Nose and Throat Hospital, Halk Sokak no:50/1, Istanbul, Turkey.
We investigated the effects of pepsin/hydrochloric acid and bile acids on eustachian tube function and eustachian tube mucosa in 40 Sprague-Dawley rats. The animals were randomly assigned to groups of 10: one group received pepsin/hydrochloric acid (pepsin/HCl group), another received human bile (human bile group), a third received a mixture of pepsin/HCl and human bile (combination group), and the fourth received isotonic saline solution (control group). Test solutions were applied transnasally three times a day for 10 days. Read More
Pancreas 2019 Jan;48(1):49-54
Department of Gastroenterology and Hepatology, Kyoto University Graduate School of Medicine, Kyoto, Japan.
Objective: Autoimmune pancreatitis (AIP) has the potential to transform into chronic pancreatitis with pancreatic stone involvement. This retrospective investigation sought to clarify the risk factors for stone formation in type 1 AIP.
Methods: Questionnaires on patients with type 1 AIP were sent to 22 high-volume medical centers across Japan to compare the clinical features of patients with and without pancreatic stone formation. Read More
J Cancer Res Ther 2018 Dec;14(Supplement):S1158-S1162
Department of General Surgery, The Second Affiliated Hospital of Kunming Medical University, Kunming 650101, Yunnan, China.
Purpose: To investigate the role of percutaneous transhepatic cholangial drainage (PTCD) combined with percutaneous endoscopic jejunostomy (PEJ) in maintaining the nutrition state in patients with advanced ampullary neoplasms.
Materials And Methods: Sixty patients who suffered from advanced ampullary neoplasms and could not tolerate internal drainage operation or biliary stent placement were enrolled. After PTCD, PEJ was implemented, and then the enteral nutrient solution + bile were instilled through PEJ tube for enteral nutrition support. Read More
Cell Mol Gastroenterol Hepatol 2018 Sep 15;7(1):197-209. Epub 2018 Sep 15.
Division of Gastroenterology and Hepatology, Mayo Clinic, Rochester, Minnesota. Electronic address:
Background & Aims: Transdifferentiation of hepatic stellate cells (HSCs) into myofibroblasts is a key event in the pathogenesis of liver fibrosis. Transforming growth factor β (TGF-β) and platelet-derived growth factor (PDGF) are canonical HSC activators after liver injury. The aim of this study was to analyze the epigenetic modulators that differentially control TGF-β and PDGF signaling pathways. Read More
J Proteome Res 2018 Dec 12. Epub 2018 Dec 12.
Primary biliary cholangitis (PBC) is a chronic autoimmune liver disease characterized by ongoing inflammatory destruction of the interlobular bile ducts, eventually leading to chronic cholestasis and biliary cirrhosis. This study primarily aims to define the metabolomic signature of PBC after comparison with healthy controls (HC). Secondly it aims at evaluating the possible metabolic association between PBC and coeliac disease (CD), an immune-mediated disorder frequently associated with PBC. Read More
Obes Surg 2018 Dec 11. Epub 2018 Dec 11.
Service de Chirurgie Générale et Endocrinienne, CHU Lille, Hôpital Huriez, Rue Michel Polonowski, 59000, Lille, France.
Background: The incidence of biliary lithiasis is increased after bariatric surgery due to rapid weight loss . Trans-oral endoscopic management in cases of common bile duct gallstone complication is not possible in patients with Roux-en-Y gastric bypass (RYGB) due to the modified anatomy. Access to the biliary tree after RYGB with a classical direct surgical approach of common bile duct and choledocoscopy can be used, but may be complicated in situations of acute cholangitis because of the fragility of common duct, or in cases of previous cholecystectomy. Read More
Ann Surg Oncol 2018 Dec 11. Epub 2018 Dec 11.
Department of Surgery, Stanford University, Stanford, CA, USA.
Background: The prevalence and characteristics of actual 5-year survivors after surgical treatment of hilar cholangiocarcinoma (HC) have not been described previously.
Methods: Patients who underwent resection for HC from 2000 to 2015 were analyzed through a multi-institutional registry from 10 U.S. Read More
Biomed Res Int 2018 14;2018:1654151. Epub 2018 Nov 14.
Université de Tunis El Manar, Faculté des Science de Tunis, LR03ES03 Laboratoire Microorganismes et Biomolécules Actives, 2092, Campus Universitaire, Tunis, Tunisia.
The research and the selection of novel probiotic strains from novel niches are receiving increased attention on their proclaimed health benefits to both humans and animals. This study aimed to evaluate the functional properties of strains from arid land living-hosts and to select strains with cholesterol-lowering property and , for use as probiotics. They were assessed for acid and bile tolerance, antibiotic susceptibility, membrane properties, antibacterial activity, antiadhesive effect against pathogens to host cell lines, and cholesterol assimilation . Read More
Int J Obes (Lond) 2018 Dec 11. Epub 2018 Dec 11.
Unit for Multidisciplinary Research in Biomedicine (UMIB), Endocrine, Cardiovascular and Metabolic Research, University of Porto, Porto, Portugal.
Background/objective: Biliopancreatic diversion with duodenal switch (BPD-DS) is the most effective bariatric intervention to treat morbid obesity and related disorders. Single-anastomosis duodeno-ileal bypass with sleeve gastrectomy (SADI-S) is a new bariatric procedure devised with the purpose of simplifying the complexity of the BPD-DS technique while maintaining its efficacy. However, whether BPD-DS and SADI-S result in similar fasting and post-prandial hormone profiles has not yet been studied. Read More
Clin Sci (Lond) 2018 Dec 11. Epub 2018 Dec 11.
Instituto de Fisiología Experimental (IFISE-CONICET) y Area Bioquímica Clínica, Facultad de Ciencias Bioquímicas y Farmacéuticas, UNR, Suipacha 570, Rosario, S2002LRL, Argentina
We previously demonstrated in and models that physiological concentrations of unconjugated bilirubin prevent oxidative stress (OS)-induced hepatocanalicular dysfunction and cholestasis. Here, we aimed to ascertain, in the whole rat, whether a similar cholestatic OS injury can be counteracted by heme oxygenase-1 (HO-1) induction that consequently elevates endogenous bilirubin (BR) levels. This was achieved through the administration of hemin, an inducer of HO-1, the rate-limiting step in BR generation. Read More
Endoscopy 2018 Dec 11. Epub 2018 Dec 11.
Department of Gastroenterology, Kyoto Second Red Cross Hospital, Kyoto, Japan.
Chemistry 2018 Dec 11. Epub 2018 Dec 11.
Localized drug delivery represents one of the most challenging uses of systems based on conductive polymer films. Typically, anionic drugs are incorporated within conductive polymers through the electrostatic interaction with the positively charged polymer. Following this approach, the synthetic glucocorticoid Dexamethasone-phosphate is often delivered from neural probes to reduce the inflammation of the surrounding tissue. Read More
Mol Nutr Food Res 2018 Dec 11:e1801040. Epub 2018 Dec 11.
J.-H. Kwon, H. G. Lee, K.-H. Seo, H. Kim, Department of Food and Nutrition, Hanyang University, Seoul.
Scope: This study aimed to determine the separate and combined effect of a prebiotic (Catechin-rich wine grape seed flour, GSF) and a probiotic (newly isolated kefir lactic acid bacteria, LAB) on hepatic steatosis of obese mice.
Methods And Results: High-fat (HF)-induced obese mice were fed HF diet containing 6% microcrystalline cellulose (MCC, control), 10% GSF, orally administrated LAB, or combination of GSF and LAB for 9 weeks. There was a significant reduction of body weight gain, and liver weights, plasma insulin concentrations, and HOMA-IR in all experimental groups compared to control. Read More
J Gastroenterol Hepatol 2018 Dec 9. Epub 2018 Dec 9.
Department of Pathology, Río Hortega Universitary Hospital, Valladolid, Spain.
J Cell Physiol 2018 Dec 7. Epub 2018 Dec 7.
Department of General Surgery, Third Xiangya Hospital, Central South University, Changsha, China.
Type II diabetes is a complex, chronic, and progressive disease. Glucagon-like peptide-1 (7-36) amide (GLP-1) is a gut hormone released from the L cells which stimulate insulin secretion and promotes insulin gene expression and β-cell growth and differentiation. Elevated levels of hormones secreted by L cells are an essential reason for diabetes improvement. Read More
J Bone Miner Res 2018 Dec 7. Epub 2018 Dec 7.
East China Normal University and Shanghai Changzheng Hospital Joint Research Center for Orthopedic Oncology, Department of orthopedic oncology, Shanghai Changzheng Hospital, Second Military Medical University, Shanghai, China.
Osteoporosis is a global bone disease characterized by reduced bone mineral density (BMD) and increased risk of fractures. The risk of developing osteoporosis increases with aging, especially after menopause in women. Discovering the signaling pathways which play a significant role in aging- and menopause-induced osteoporosis should accelerate osteoporosis drug discovery. Read More
Methods Mol Biol 2019 ;1905:175-185
Institute of Industrial Science, The University of Tokyo, Tokyo, Japan.
Incorporation of bile drainage system into engineered liver tissue is an important issue to advance liver regenerative medicine. Our group reported that three-dimensional (3D) coculture of fetal liver cells (FLCs) and adult rat biliary epithelial cells (BECs) allows reconstruction of hepatic spheroids that possess bile ductular structures. In this chapter, we describe the detailed protocol to isolate FLCs and BECs and to generate the spheroids with bile drainage system using these two types of primary cells. Read More
Methods Mol Biol 2019 ;1905:143-153
Department of Molecular Life Sciences, Tokai University School of Medicine, Isehara, Kanagawa, Japan.
Inducible pluripotent stem (iPS) cells are multipotent stem cells that are produced by gene transfer of reprogramming factors to somatic cells. They are thought to be an important source of regenerative medicine because of their pluripotency and self-renewal ability. Although the liver has high regeneration ability, continuous death of hepatocytes due to chronic inflammation leads to liver cirrhosis and liver carcinoma. Read More
Methods Mol Biol 2019 ;1905:29-41
Department of Tissue Development and Regeneration, Research Institute for Frontier Medicine, Sapporo Medical University School of Medicine, Sapporo, Japan.
This protocol showed procedures to isolate and expand small hepatocytes (SHs), as hepatocytic progenitor cells, from a rat liver. SHs are identified as a subpopulation of mature hepatocytes in a healthy liver. SHs can proliferate to form colonies in serum-free medium on hyaluronic acid-coated dishes, of which cells show CD44 positivity (CD44 SHs). Read More
Transplant Direct 2018 Nov 23;4(11):e400. Epub 2018 Oct 23.
Division of Organ Transplantation, Department of Surgery, UMass Memorial Medical Center, University of Massachusetts, Worcester, MA.
Background: It remains controversial if arterial perfusion in addition to portal vein perfusion during machine preservation improves liver graft quality. Comparative studies using both techniques are lacking. We studied the impact of using single or dual machine perfusion of donation after circulatory death rat livers. Read More