43,312 results match your criteria Becker Melanosis


Identification of B cell epitopes enhanced by protein unfolding and aggregation.

Mol Immunol 2018 Dec 11;105:181-189. Epub 2018 Dec 11.

Lydia Becker Institute of Immunology and Inflammation, School of Biological Sciences, Faculty of Biology, Medicine and Health, Manchester Academic Health Science Centre, The University of Manchester, Oxford Road, Manchester, UK. Electronic address:

Aggregation of therapeutic proteins is a key factor in the generation of unwanted immunogenicity, and can result in reduced serum half-life, neutralization of function and adverse health effects. There is currently little information regarding how aggregates interact with B-cell receptors or cognate antibodies at the protein sequence level, or whether non-native, aggregate-induced epitopes predominate in these interactions. Using an antibody fragment (single chain antibody variable fragment; scFv) that forms aggregates readily at low temperature, anti-scFv IgG antibody responses were generated by intraperitoneal injection of BALB/c strain mice with monomer or aggregate preparations. Read More

View Article
December 2018
1 Read

Dosimetric effects of the kV based image-guided radiation therapy of prone breast external beam radiation: Towards the optimized imaging frequency.

J Appl Clin Med Phys 2018 Dec 14. Epub 2018 Dec 14.

Department of Radiation Oncology, University of Maryland School of Medicine, Baltimore, MD, 21201, USA.

Purpose: For prone breast treatment, daily image-guided radiation therapy (IGRT) allows couch shifting to correct breast position relative to the treatment field. This work investigates the dosimetric effect of reducing kV imaging frequencies and the feasibility of optimizing the frequency using patient anatomy or their first 3-day shifts.

Method: Thirty-seven prone breast patients who had been treated with skin marker alignment followed by daily kV were retrospectively analyzed. Read More

View Article
December 2018
1 Read

Single posttranslational modifications in the central repeat domains of Tau4 impact its aggregation and tubulin binding.

Angew Chem Int Ed Engl 2018 Dec 14. Epub 2018 Dec 14.

University of Vienna, Institute of Biological Chemistry, Währinger Str. 38, 1090, Vienna, AUSTRIA.

A variety of methods has been employed to study the impact of posttranslational modifications on Tau protein function. Here, we describe a semisynthesis strategy that enables selective modification within the central repeat domain of Tau4 (residues 291-321) comprising a major interaction motive with tubulin as well as one of the key hexapeptides involved in Tau aggregation. This strategy has led to preparation of four semisynthetic Tau variants with phosphoserine residues in different positions and one with a so far largely ignored carboxymethyllysine modification that results from a non-enzymatic posttranslational modification (nPTM). Read More

View Article
December 2018

Unilateral neglect post stroke: Eye movement frequencies indicate directional hypokinesia while fixation distributions suggest compensational mechanism.

Brain Behav 2018 Dec 12:e01170. Epub 2018 Dec 12.

Department of Psychology, University of Oslo, Oslo, Norway.

Introduction: Eye movements and spatial attention are closely related, and eye-tracking can provide valuable information in research on visual attention. We investigated the pathology of overt attention in right hemisphere (RH) stroke patients differing in their severity of neglect symptoms by using eye-tracking during a dynamic attention task.

Methods: Eye movements were recorded in 26 RH stroke patients (13 with and 13 without unilateral spatial neglect, and a matched group of 26 healthy controls during a Multiple Object Tracking task. Read More

View Article
December 2018
3 Reads

Left ventricular noncompaction cardiomyopathy in Duchenne muscular dystrophy carriers.

J Cardiol Cases 2015 Jan 3;11(1):7-9. Epub 2014 Oct 3.

Cincinnati Children's Hospital Medical Center, The Heart Institute - Pediatric Cardiology, Cincinnati, OH, USA.

Duchenne and Becker muscular dystrophies are X-linked hereditary myopathies secondary to a dystrophinopathy resulting in progressive cardiomyopathy and heart failure. The most commonly associated cardiac involvements in these patients are dilated cardiomyopathy and conduction abnormalities; however, recent studies have shown a high prevalence of left ventricular noncompaction cardiomyopathy in patients with Duchenne muscular dystrophy. Furthermore, there is increasing awareness of cardiomyopathy in female heterozygous dystrophinopathy carriers. Read More

View Article
January 2015

Fast 3D Fluid Registration of Brain Magnetic Resonance Images.

Proc SPIE Int Soc Opt Eng 2008 Mar;6916

Laboratory of Neuro Imaging, Department of Neurology, UCLA School of Medicine, Los Angeles, CA 90095, USA.

Fluid registration is widely used in medical imaging to track anatomical changes, to correct image distortions, and to integrate multi-modality data. Fluid mappings guarantee that the template image deforms smoothly into the target, without tearing or folding, even when large deformations are required for accurate matching. Here we implemented an intensity-based fluid registration algorithm, accelerated by using a filter designed by Bro-Nielsen and Gramkow. Read More

View Article

Clinical Features and Treatment of Down Syndrome Arthropathy: Experience from Two US Tertiary Hospitals.

Paediatr Drugs 2018 Dec 13. Epub 2018 Dec 13.

Division of Rheumatology, Children's Mercy Kansas City, 2401 Gillham Road, Kansas City, MO, 64108, USA.

Background: Arthropathy of Down syndrome (DA) is largely under-recognized, with an average 2-year delay in diagnosis. Most patients present with polyarthritis, and treatment has historically been challenging.

Objectives: Our objective was to investigate the clinical features and treatment of DA in the largest cohort reported to date. Read More

View Article
December 2018
1 Read

Prevention of Arrhythmia Device Infection Trial: The PADIT Trial.

J Am Coll Cardiol 2018 Dec;72(24):3098-3109

Population Health Research Institute, Hamilton Health Sciences, McMaster University, Hamilton, Ontario, Canada.

Background: Infection of implanted medical devices has catastrophic consequences. For cardiac rhythm devices, pre-procedural cefazolin is standard prophylaxis but does not protect against methicillin-resistant gram-positive organisms, which are common pathogens in device infections.

Objective: This study tested the clinical effectiveness of incremental perioperative antibiotics to reduce device infection. Read More

View Article
December 2018
16.503 Impact Factor

Sequential treatment of ADHD in mother and child (AIMAC study): importance of the treatment phases for intervention success in a randomized trial.

BMC Psychiatry 2018 Dec 13;18(1):388. Epub 2018 Dec 13.

Department of Child and Adolescent Psychiatry, Psychosomatics and Psychotherapy, Center for Mental Health, University Hospital of Würzburg, Würzburg, Germany.

Background: The efficacy of parent-child training (PCT) regarding child symptoms may be reduced if the mother has attention-deficit/hyperactivity disorder (ADHD). The AIMAC study (ADHD in Mothers and Children) aimed to compensate for the deteriorating effect of parental psychopathology by treating the mother (Step 1) before the beginning of PCT (Step 2). This secondary analysis was particularly concerned with the additional effect of the Step 2 PCT on child symptoms after the Step 1 treatment. Read More

View Article
December 2018
3 Reads

Multiple Exon Skipping in the Duchenne Muscular Dystrophy Hot Spots: Prospects and Challenges.

J Pers Med 2018 Dec 7;8(4). Epub 2018 Dec 7.

Department of Medical Genetics, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, AB T6G2H7, Canada.

Duchenne muscular dystrophy (DMD), a fatal X-linked recessive disorder, is caused mostly by frame-disrupting, out-of-frame deletions in the () gene. Antisense oligonucleotide-mediated exon skipping is a promising therapy for DMD. Exon skipping aims to convert out-of-frame mRNA to in-frame mRNA and induce the production of internally-deleted dystrophin as seen in the less severe Becker muscular dystrophy. Read More

View Article
December 2018

Impaired Glucose Tolerance in Adults with Duchenne and Becker Muscular Dystrophy.

Nutrients 2018 Dec 7;10(12). Epub 2018 Dec 7.

Research Centre for Musculoskeletal Science & Sports Medicine, Department of Exercise and Sport Science, Manchester Metropolitan University Cheshire, Crewe CW1 5DU, UK.

The aim of this study was to determine the response to an oral glucose tolerance test (OGTT) in adult males with Becker muscular dystrophy (BMD) and Duchenne muscular dystrophy (DMD), and to investigate whether body composition contributes to any variance in the glucose response. Twenty-eight adult males with dystrophinopathy (BMD, = 13; DMD, = 15) and 12 non-dystrophic controls, ingested 75 g oral anhydrous glucose solution. Fingertip capillary samples were assessed for glucose at 30-min intervals over 2-h post glucose ingestion. Read More

View Article
December 2018

Hepatic hexokinase domain containing 1 (HKDC1) improves whole body glucose tolerance and insulin sensitivity in pregnant mice.

Biochim Biophys Acta Mol Basis Dis 2018 Dec 10. Epub 2018 Dec 10.

Division of Endocrinology, Diabetes and Metabolism, Department of Medicine, University of Illinois at Chicago, IL, USA; Jesse Brown Veterans Affairs Medical Center, Chicago, IL, USA. Electronic address:

Hexokinase domain containing 1, a recently discovered putative fifth hexokinase, is hypothesized to play key roles in glucose metabolism. Specifically, during pregnancy in a recent genome wide association study (GWAS), a strong correlation between HKDC1 and 2-h plasma glucose in pregnant women from different ethnic backgrounds was shown. Our earlier work also reported diminished glucose tolerance during pregnancy in our whole body HKDC1 heterozygous mice. Read More

View Article
December 2018

[Pilot study on drinking and voiding management in child day-care facilities in the district of Garmisch-Partenkirchen].

Urologe A 2018 Dec 12. Epub 2018 Dec 12.

Klinik für Urologie und Kinderurologie, Universitätsklinik Ulm, Prittwitzstr. 43, 89075, Ulm, Deutschland.

Background And Objectives: The development of child day care is currently the focus of a public discussion. More and more very young children are cared for in a day-care facility rather than by the family. Therefore, key aspects of education are shifted into these institutions. Read More

View Article
December 2018

Dynamics of Colon Monocyte and Macrophage Activation During Colitis.

Front Immunol 2018 27;9:2764. Epub 2018 Nov 27.

Lydia Becker Institute of Immunology and Inflammation, Manchester Collaborative Centre for Inflammation Research, Faculty of Biology, Medicine and Health, Manchester Academic Health Science Centre, University of Manchester, Manchester, United Kingdom.

Macrophages are pivotal in coordinating a range of important processes in the intestines, including controlling intracellular infections and limiting damaging inflammation against the microbiota. However, it is not clear how gut macrophages, relative to recruited blood monocytes and other myeloid cells, contribute to the intestinal inflammatory milieu, nor how macrophages and their monocyte precursors mediate recruitment of other immune cells to the inflamed intestine. Myeloid cell populations isolated from colonic inflammatory bowel disease (IBD) or murine dextran sulphate sodium (DSS) induced colitis were assessed using flow cytometry and compared to healthy controls. Read More

View Article
November 2018

Complex Structural Variant Associated with Non-syndromic Canine Retinal Degeneration.

G3 (Bethesda) 2018 Dec 12. Epub 2018 Dec 12.

University of Pennsylvania.

Rod and cone photoreceptors are specialized retinal neurons that have a fundamental role in visual perception, capturing light and transducing it into a neuronal signal. Aberrant functioning of rod and/or cone photoreceptors can ultimately lead to progressive degeneration and eventually blindness. In man, many rod and rod-cone degenerative diseases are classified as forms of retinitis pigmentosa (RP). Read More

View Article
December 2018
1 Read

Social Cognition in Children With ADHD.

J Atten Disord 2018 Dec 13:1087054718816157. Epub 2018 Dec 13.

1 University of Nevada, Las Vegas, USA.

Objective: Despite evidence of social skill deficits in children with ADHD, there is no consensus regarding a social cognitive profile and whether these skills predict behavior. Therefore, a comprehensive battery was used to investigate the relationship between social cognition and behavioral functioning.

Method: Children ages 7 to 13 with ADHD ( n = 25) and controls ( n = 25) completed tests assessing social cognitive domains (affect recognition and theory of mind [ToM]). Read More

View Article
December 2018

Dropping acid: why is cystic fibrosis mucus abnormal?

Eur Respir J 2018 Dec 6;52(6). Epub 2018 Dec 6.

Wellcome Trust Centre for Cell-Matrix Research and Lydia Becker Institute of Immunology and Inflammation, School of Biological Sciences, Faculty of Biology, Medicine and Health, University of Manchester, Manchester Academic Health Sciences Centre, Manchester, UK.

View Article
December 2018

The proteome microenvironment determines the protective effect of preconditioning in cisplatin-induced acute kidney injury.

Kidney Int 2018 Nov 29. Epub 2018 Nov 29.

Department II of Internal Medicine, University of Cologne, Cologne, Germany; Center for Molecular Medicine, University of Cologne, Cologne, Germany; Cologne Excellence Cluster on Cellular Stress Responses in Aging Associated Diseases (CECAD), University of Cologne, Cologne, Germany; Systems Biology of Ageing Cologne (Sybacol), University of Cologne, Cologne, Germany. Electronic address:

Acute kidney injury (AKI) leads to significant morbidity and mortality; unfortunately, strategies to prevent or treat AKI are lacking. In recent years, several preconditioning protocols have been shown to be effective in inducing organ protection in rodent models. Here, we characterized two of these interventions-caloric restriction and hypoxic preconditioning-in a mouse model of cisplatin-induced AKI and investigated the underlying mechanisms by acquisition of multi-layered omic data (transcriptome, proteome, N-degradome) and functional parameters in the same animals. Read More

View Article
November 2018

Factor cooperation for chromosome discrimination in Drosophila.

Nucleic Acids Res 2018 Dec 12. Epub 2018 Dec 12.

Molecular Biology Division, Biomedical Center, Faculty of Medicine and Center for Integrated Protein Science Munich (CIPSM), Ludwig-Maximilians-Universität Munich, 82151 Martinsried, Germany.

Transcription regulators select their genomic binding sites from a large pool of similar, non-functional sequences. Although general principles that allow such discrimination are known, the complexity of DNA elements often precludes a prediction of functional sites. The process of dosage compensation in Drosophila allows exploring the rules underlying binding site selectivity. Read More

View Article
December 2018
2 Reads

Chasing red herrings: Can visual distracters extend the time children take to open child resistant vials?

PLoS One 2018 12;13(12):e0207738. Epub 2018 Dec 12.

School of Packaging, Michigan State University, East Lansing, MI, United States of America.

Background: Unintentional exposure to medications is a noted problem in pediatric populations despite the prevalent use of child-resistant (CR) packaging and educational campaigns informing consumers about appropriate storage.

Objective: Conduct a proof-of concept study that evaluates how package designs that engage the attention of children in meaningless ways affect opening time and number of openings.

Study Design: Non-CR vials with or without distracters were provided to 108 children (24-51 months) in pairs. Read More

View Article
December 2018

Muscle specific stress fibers give rise to sarcomeres in cardiomyocytes.

Elife 2018 Dec 12;7. Epub 2018 Dec 12.

Department of Cell and Developmental Biology, Vanderbilt University, Nashville, United States.

The sarcomere is the contractile unit within cardiomyocytes driving heart muscle contraction. We sought to test the mechanisms regulating actin and myosin filament assembly during sarcomere formation. Therefore, we developed an assay using human cardiomyocytes to monitor sarcomere assembly. Read More

View Article
December 2018
1 Read

Nursing care practices for people with Chronic Noncommunicable Diseases.

Rev Bras Enferm 2018 ;71(suppl 6):2643-2649

Universidade Federal de Santa Catarina. Florianópolis, Santa Catarina, Brazil.

Objective: To understand care practices for people with Chronic Noncommunicable Disease, developed by Primary Health Care nurses in a municipality in southern Brazil.

Method: Qualitative study, developed through the Discourse of the Collective Subject. Twenty-three nurses from the Family Health Strategy participated. Read More

View Article
January 2018

One-Tube Multicolor Flow Cytometry Assay (OTMA) for Comprehensive Immunophenotyping of Peripheral Blood.

Methods Mol Biol 2019 ;1904:189-212

Department of Radiation Oncology, Universitätsklinikum Erlangen, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany.

Recent improvements in the flow cytometry technology allow the determination of the general immune status through the development of multicolor immunofluorescence panels. The one-tube multicolor flow cytometry assay (OTMA) that is presented here identifies 20 different, clinically relevant immune cell subsets and three common activation markers. Thereby, a comprehensive immune status that covers all major immune cells is easily obtained. Read More

View Article
January 2019

Quality improvement of functional diagnostics in dentistry through computer-aided diagnosis: a randomized controlled trial.

Int J Comput Dent 2018 ;21(4):281-294

Functional diagnostic examinations such as clinical functional analysis and manual structural analysis ('orthopedic tests') allow the dentist to establish a structured diagnosis. Previously, the process of correlating findings with the appropriate diagnoses was guided by human thought processes alone. The experimental diagnostic randomized controlled trial (RCT) in this study investigated whether computer-aided diagnosis (CADx) of temporomandibular disorders (TMD) offers quality advantages over traditional diagnosis (TRAD). Read More

View Article
January 2018

Effect of On-Demand Oral Pre-exposure Prophylaxis With Tenofovir/Emtricitabine on Herpes Simplex Virus-1/2 Incidence Among Men Who Have Sex With Men: A Substudy of the ANRS IPERGAY Trial.

Open Forum Infect Dis 2018 Nov 8;5(11):ofy295. Epub 2018 Nov 8.

Institut National de la Santé et de la Recherche Médicale (INSERM) Unité, Centre National de la Recherche Scientifique Unité Mixte de Recherche, Institut Universitaire d'Hématologie, Sorbonne Paris Cité, Université Paris Diderot, Hôpital Saint Louis, France.

We evaluated the impact of on-demand oral tenofovir disoproxil fumarate (TDF)/emtricitabine (FTC) for pre-exposure prophylaxis (PrEP) on herpes simplex virus (HSV)-1/2 incidence among men who have sex with men (MSM) enrolled in the ANRS IPERGAY trial. Serum samples were tested at baseline and at the last visit for HSV-1/2 antibodies. Overall HSV-1 incidence was 11. Read More

View Article
November 2018

Non-canonical nucleosides and chemistry of the emergence of life.

Nat Commun 2018 12 12;9(1):5174. Epub 2018 Dec 12.

Department of Chemistry at the Ludwig-Maximilians Universität München, Butenandtstr. 5-13, 81377, Munich, Germany.

Prebiotic chemistry, driven by changing environmental parameters provides canonical and a multitude of non-canonical nucleosides. This suggests that Watson-Crick base pairs were selected from a diverse pool of nucleosides in a pre-Darwinian chemical evolution process. Read More

View Article
December 2018

The diversity of population responses to environmental change.

Ecol Lett 2018 Dec 9. Epub 2018 Dec 9.

Université Lyon 1, CNRS, UMR 5558, Laboratoire de Biométrie et Biologie Evolutive, F-69622, Villeurbanne, France.

The current extinction and climate change crises pressure us to predict population dynamics with ever-greater accuracy. Although predictions rest on the well-advanced theory of age-structured populations, two key issues remain poorly explored. Specifically, how the age-dependency in demographic rates and the year-to-year interactions between survival and fecundity affect stochastic population growth rates. Read More

View Article
December 2018
1 Read

Oxytocin Modulates the Intrinsic Dynamics Between Attention-Related Large-Scale Networks.

Cereb Cortex 2018 Dec 7. Epub 2018 Dec 7.

The Clinical Hospital of Chengdu Brain Science Institute, MOE Key Laboratory for Neuroinformation, University of Electronic Science and Technology of China, Chengdu, China.

Attention And Salience Processing Have Been Linked To The Intrinsic Between- And Within-network Dynamics Of Large-scale Networks Engaged In Internal (default Network [dn]) And External Attention Allocation (dorsal Attention Network [dan] And Salience Network [sn]). The Central Oxytocin (oxt) System Appears Ideally Organized To Modulate Widely Distributed Neural Systems And To Regulate The Switch Between Internal Attention And Salient Stimuli In The Environment. The Current Randomized Placebo (plc)-controlled Between-subject Pharmacological Resting-state Fmri Study In N = 187 (oxt, N = 94; Plc, N = 93; Single-dose Intranasal Administration) Healthy Male And Female Participants Employed An Independent Component Analysis Approach To Determine The Modulatory Effects Of Oxt On The Within- And Between-network Dynamics Of The Dan–sn–dn Triple Network System. Read More

View Article
December 2018

Black aspergilli: A remaining challenge in fungal taxonomy?

Med Mycol 2018 Dec 11. Epub 2018 Dec 11.

BCCM/IHEM collection, Mycology and Aerobiology, Sciensano, J. Wytsmanstraat 14, 1050 Brussels, Belgium.

Aspergillus section Nigri is a taxonomically difficult but medically and economically important group. In this study, an update of the taxonomy of A. section Nigri strains within the BCCM/IHEM collection has been conducted. Read More

View Article
December 2018

MODELING DNA DAMAGE BY PHOTONS AND LIGHT IONS OVER ENERGY RANGES USED IN MEDICAL APPLICATIONS.

Radiat Prot Dosimetry 2018 Dec 11. Epub 2018 Dec 11.

Institute of Radiation Protection, Department of Radiation Sciences, Helmholtz Zentrum München-German Research Center for Environmental Health, Ingolstädter Landstr. 1, Neuherberg, Germany.

Comprehensive track structure-based simulations of DNA damage induced in human cells by photons (5 keV-1.3 MeV) and light ions (0.25-512 MeV/u) were performed with PARTRAC. Read More

View Article
December 2018

Respiratory failure requiring mechanical ventilation in critically ill adults in Ghana: A prospective observational study.

Afr J Emerg Med 2018 Dec 20;8(4):155-157. Epub 2018 Sep 20.

Department of Emergency Medicine, University of Florida, Gainesville, FL, USA.

Introduction: Respiratory failure is commonly seen in African emergency centres and intensive care units, but little is known about the need for intubation and mechanical ventilation.

Methods: From April to October 2017, we recorded the number of patients intubated and ventilated in the emergency centre and intensive care unit at Komfo Anokye Teaching Hospital in Kumasi, Ghana on a daily basis. We assessed patients for presence of acute respiratory distress syndrome (ARDS) using the Kigali Modification of the Berlin ARDS criteria. Read More

View Article
December 2018

Do Academic Health Care Systems Really Value Education? A Survey of Academic Plastic Surgeons.

Plast Reconstr Surg Glob Open 2018 Oct 16;6(10):e1948. Epub 2018 Oct 16.

Case Western Reserve University School of Medicine, Department of Plastic Surgery, Cleveland, Ohio.

Background: Although education is critical to the mission of academic medical centers in the United States, it is often not quantified and monetized as are their research and clinical missions. We undertook this survey to assess the perceived valuation of educational endeavors of plastic surgeon faculty at the U.S. Read More

View Article
October 2018
1 Read

Draft Genome Sequence of Salmonella enterica subsp. enterica Serovar Enteritidis MS 501, a Potential Human Pathogen Isolated from Red Lettuce (Lactuca sativa var. capitata) in Karlsruhe, Germany.

Microbiol Resour Announc 2018 Aug 2;7(4). Epub 2018 Aug 2.

Department of Safety and Quality of Fruit and Vegetables, Max Rubner-Institut, Federal Research Institute of Nutrition and Food, Karlsruhe, Germany.

We report here the genome sequence of Salmonella enterica subsp. enterica serovar Enteritidis MS 501, a potential human pathogen isolated from red lettuce (Lactuca sativa var. capitata) in Karlsruhe, Germany. Read More

View Article

Review of the Neotropical genus with descriptions of three new species-group taxa (Hemiptera, Heteroptera, Pentatomidae).

Zookeys 2018 15(796):347-395. Epub 2018 Nov 15.

Florida State Collection of Arthropods, 2606 S. Dundee St., 33629 Tampa, FL, USA Florida State Collection of Arthropods Tampa United States of America.

The genus Bergroth, 1911 (Hemiptera: Heteroptera: Pentatomidae: Pentatominae: Chlorocorini) is redescribed and five species-group taxa are recognized, keyed, their diagnostic characters illustrated, and the distribution reviewed. Among the five taxa, two species and one subspecies are recognized as new: Bergroth, 1911 (Brazil, Ecuador, French Guiana, Guyana, Peru, Suriname), (Belize, Costa Rica, Guatemala, Mexico, Panama), (French Guiana), Becker & Grazia-Vieira, 1971 (Bolivia, Brazil, Ecuador, Peru), and (Guyana). The structure of the male genital capsule was found to be the only reliable character for identifying species-group taxa. Read More

View Article
November 2018

Zoonotic multidrug-resistant microorganisms among small companion animals in Germany.

PLoS One 2018 7;13(12):e0208364. Epub 2018 Dec 7.

Institute of Medical Microbiology, University Hospital Münster, Münster, Germany.

Antimicrobial multidrug-resistant microorganisms (MDRO) can be transmitted between companion animals and their human owners. Aim of this study was to determine the prevalence of extended spectrum beta-lactamase-producing Enterobacteriaceae (ESBL-E) and Staphylococcus aureus including methicillin-resistant S. aureus (MRSA) in different companion animal species. Read More

View Article
December 2018

A DNA-based fluorescent reporter maps HOCl production in the maturing phagosome.

Nat Chem Biol 2018 Dec 10. Epub 2018 Dec 10.

Department of Chemistry, The University of Chicago, Chicago, IL, USA.

Phagocytes destroy pathogens by trapping them in a transient organelle called the phagosome, where they are bombarded with reactive oxygen species (ROS) and reactive nitrogen species (RNS). Imaging reactive species within the phagosome would directly reveal the chemical dynamics underlying pathogen destruction. Here we introduce a fluorescent, DNA-based combination reporter, cHOClate, which simultaneously images hypochlorous acid (HOCl) and pH quantitatively. Read More

View Article
December 2018
1 Read

Genome-wide mega-analysis identifies 16 loci and highlights diverse biological mechanisms in the common epilepsies.

Authors:

Nat Commun 2018 12 10;9(1):5269. Epub 2018 Dec 10.

The epilepsies affect around 65 million people worldwide and have a substantial missing heritability component. We report a genome-wide mega-analysis involving 15,212 individuals with epilepsy and 29,677 controls, which reveals 16 genome-wide significant loci, of which 11 are novel. Using various prioritization criteria, we pinpoint the 21 most likely epilepsy genes at these loci, with the majority in genetic generalized epilepsies. Read More

View Article
December 2018

Cohesin-mediated NF-κB signaling limits hematopoietic stem cell self-renewal in aging and inflammation.

J Exp Med 2018 Dec 7. Epub 2018 Dec 7.

Leibniz Institute on Aging, Fritz Lipmann Institute (FLI), Jena, Germany

Organism aging is characterized by increased inflammation and decreased stem cell function, yet the relationship between these factors remains incompletely understood. This study shows that aged hematopoietic stem and progenitor cells (HSPCs) exhibit increased ground-stage NF-κB activity, which enhances their responsiveness to undergo differentiation and loss of self-renewal in response to inflammation. The study identifies /cohesin as a critical mediator of NF-κB signaling, which increases chromatin accessibility in the vicinity of NF-κB target genes in response to inflammation. Read More

View Article
December 2018
12.515 Impact Factor

Self-scanned HIFU ablation of moving tissue using real-time hybrid US-MR imaging.

IEEE Trans Biomed Eng 2018 Dec 5. Epub 2018 Dec 5.

Objective: High Intensity Focused Ultrasound (HIFU) treatment in the abdominal cavity is challenging due to the respiratory motion. In the self-scanning HIFU ablation method the focal spot is kept static and the heating pattern is obtained through natural tissue motion. This paper describes a novel approach for modulating the HIFU power during self-scanning in order to compensate for the effect of tissue motion on thermal build-up. Read More

View Article
December 2018

Validation of the self-administered version of the international Restless Legs Syndrome study group severity rating scale - The sIRLS.

Sleep Med 2018 Oct 29;54:94-100. Epub 2018 Oct 29.

The International Restless Legs Syndrome Study Group, Rochester, MN, USA.

Introduction: The International Restless Legs Study Group (IRLSSG) has developed the IRLS (International Restless Legs Syndrome Severity Scale) and validated it as a clinician/researcher administered scale to be used when both patient and examiner are present. The IRLSSG recognized the need for a self-completing scale that can be used economically in clinical practice and in large population-based studies. In this study the validity and the reliability of the IRLS as a self-administered scale (sIRLS) is assessed. Read More

View Article
October 2018

High density placental mesenchymal stromal cells provide neuronal preservation and improve motor function following in utero treatment of ovine myelomeningocele.

J Pediatr Surg 2018 Oct 4. Epub 2018 Oct 4.

Department of Surgery, University of California Davis, Sacramento, CA, USA.

Purpose: The purpose of this study was to determine whether seeding density of placental mesenchymal stromal cells (PMSCs) on extracellular matrix (ECM) during in utero repair of myelomeningocele (MMC) affects motor function and neuronal preservation in the ovine model.

Methods: MMC defects were surgically created in 33 fetuses and repaired following randomization into four treatment groups: ECM only (n = 10), PMSC-ECM (42 K cells/cm) (n = 8), PMSC-ECM (167 K cells/cm) (n = 7), or PMSC-ECM (250-300 K cells/cm) (n = 8). Motor function was evaluated using the Sheep Locomotor Rating Scale (SLR). Read More

View Article
October 2018
2 Reads

Wounding-Induced Stomatal Closure Requires Jasmonate-Mediated Activation of GORK K Channels by a Ca Sensor-Kinase CBL1-CIPK5 Complex.

Dev Cell 2018 Dec 3. Epub 2018 Dec 3.

Molekulare Pflanzenphysiologie & Biophysik, Julius-Maximilians-Universität Würzburg, Julius-von-Sachs-Platz 2, Würzburg 97082, Germany. Electronic address:

Guard cells integrate various hormone signals and environmental cues to balance plant gas exchange and transpiration. The wounding-associated hormone jasmonic acid (JA) and the drought hormone abscisic acid (ABA) both trigger stomatal closure. In contrast to ABA however, the molecular mechanisms of JA-induced stomatal closure have remained largely elusive. Read More

View Article
December 2018

Immediate breast reconstruction in high-risk cases using an anatomically shaped permanent expandable implant.

J Plast Reconstr Aesthet Surg 2018 Nov 13. Epub 2018 Nov 13.

Plastic and Reconstructive Breast Surgery Unit, Department of Plastic Surgery, Tel-Aviv Sourasky Medical Center, Sackler Faculty of Medicine, Tel-Aviv University, 6 Weizmann St., Tel-Aviv 6423906, Israel. Electronic address:

Background: Anatomically shaped permanent expandable implants are dual-chambered devices, which allow their postoperative expansion for the adjustment of volume. Their use is optional when 1-stage immediate breast reconstruction (IBR) direct to permanent implant is not feasible. The aim of this study is to present one center's long-term experience with the anatomical Becker expandable implant in a large series of patients who underwent either IBR or a salvage procedure for IBR with the device. Read More

View Article
November 2018

Comment on Malmborn, Becker, and Klintö: Problems With Reliability of Speech Variables for Use in Quality Registries for Cleft Lip and Palate-Experiences From the Swedish Cleft Lip and Palate Registry, CPCJ 2018.

Authors:
Anette Lohmander

Cleft Palate Craniofac J 2018 Dec 9:1055665618817654. Epub 2018 Dec 9.

1 Division of Speech & Language Pathology, CLINTEC, Karolinska Institutet, Stockholm, Sweden.

View Article
December 2018

Early impact of pulmonary stereotactic fractionated body radiotherapy (SBRT) on Quality of Life (QoL): Benefit for patients with low initial scores (STRIPE trial).

J Thorac Oncol 2018 Dec 3. Epub 2018 Dec 3.

Department of Radiation Oncology, Medical Center, Faculty of Medicine, University of Freiburg, Germany; German Cancer Consortium (DKTK) Partner Site Freiburg, Germany; German Cancer Research Center (DKFZ), Heidelberg, Germany.

Introduction: Quality of life (QoL) of comorbid patients with pulmonary malignancies is a key issue in considering stereotactic fractionated body radiotherapy (SBRT) indication. This study investigates the early impact of SBRT on QoL.

Methods: 100 patients with pulmonary lesions were treated with SBRT from 02/2011 to 11/2014 within the prospective, monocenter, phase II STRIPE trial. Read More

View Article
December 2018
3 Reads

Concurrent Developmental Course of Sleep Problems and Emotional/behavioral Problems in Childhood and Adolescence as Reflected by the Dysregulation Profile.

Sleep 2018 Dec 6. Epub 2018 Dec 6.

Centre and Discipline of Child and Adolescent Psychiatry, Psychosomatics and Psychotherapy, The University of Western Australia, Perth, WA, Australia.

Objectives: Longitudinal data on the course and relationship of concurrent psychopathology in youth are scarce but are of need for better practical patient care and prevention. This study explores the course of (and relationships over time) between sleep problems and concurrent dimensional difficulties relating to anxiety/depression, attention deficiency and aggressive behaviors in childhood and adolescence. The latter three may jointly form a broad syndrome, the dysregulation profile. Read More

View Article
December 2018
2 Reads

Vertebral body insufficiency fractures: detection of vertebrae at risk on standard CT images using texture analysis and machine learning.

Eur Radiol 2018 Dec 5. Epub 2018 Dec 5.

Institute of Diagnostic and Interventional Radiology, University Hospital Zurich, Ramistrasse 100, 8091, Zurich, Switzerland.

Purpose: To evaluate the diagnostic performance of bone texture analysis (TA) combined with machine learning (ML) algorithms in standard CT scans to identify patients with vertebrae at risk for insufficiency fractures.

Materials And Methods: Standard CT scans of 58 patients with insufficiency fractures of the spine, performed between 2006 and 2013, were analyzed retrospectively. Every included patient had at least two CT scans. Read More

View Article
December 2018
1 Read