431,208 results match your criteria Basic research in cardiology[Journal]


Evaluation of Nrf2 with Exposure to Nanoparticles.

Methods Mol Biol 2019 ;1894:229-246

Fujian Provincial Key Laboratory of Environmental Factors and Cancer, School of Public Health, Fujian Medical University, Fuzhou, P. R. China.

Transcription factor Nrf2, nuclear factor (erythroid-derived 2)-like 2, is considered a master regulator of redox homeostasis and plays a central role in antioxidant and anti-inflammatory defence. It has been largely reported that oxidative stress is implicated in nanoparticle-induced toxicity with the involvement of Nrf2. Several basic methods for Nrf2 evaluation with exposure to nanoparticles are described in this chapter including real-time reverse transcription-polymerase chain reaction (RT-PCR), western blotting, immunofluorescence staining, electrophoretic mobility shift assay, DNase I footprinting, dimethylsulfate footprinting, protein pulse-chase analysis, and tert-butylhydroquinone treatment. Read More

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January 2019

Determination of Phosphorylated Histone H2AX in Nanoparticle-Induced Genotoxic Studies.

Methods Mol Biol 2019 ;1894:145-159

Department of Environmental and Occupational Health Sciences, School of Public Health and Information Sciences, University of Louisville, Louisville, KY, USA.

DNA double-strand breaks (DSBs), one of the most severe lesions of DNA damage triggered by various genotoxic insults, can lead to chromosome change, genomic instability, and even tumorigenesis if not repaired efficiently. In response to DNA damage, histone H2AX molecules are rapidly phosphorylated at serine 139 near the site of DNA DSBs and form γ-H2AX foci. As an early important cellular event linked to DNA damage and repair, γ-H2AX is a highly sensitive biomarker for "monitoring" DNA damage and consequently is a useful tool in genetic toxicology screen. Read More

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January 2019
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Methods for Evaluation of Ovarian Granulosa Cells with Exposure to Nanoparticles.

Methods Mol Biol 2019 ;1894:73-81

Fujian Provincial Key Laboratory of Environmental Factors and Cancer, School of Public Health, Fujian Medical University, Fuzhou, People's Republic of China.

Increasing evidence demonstrates that a substantial number of chemicals including nanoparticles have potential toxic effects on human and wildlife reproductive health. Ovarian granulosa cells (GCs), the functional unit of the ovary, play a pivotal role in the regulation of ovarian reproductive health in females. They are considered to be the main target by the chemicals at all stages of their development. Read More

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January 2019
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Visuospatial Integration and Hand-Tool Interaction in Cognitive Archaeology.

Curr Top Behav Neurosci 2018 Dec 15. Epub 2018 Dec 15.

Sociograph Marketing Science Consulting, Valladolid, Spain.

Testing cognitive hypotheses in extinct species can be challenging, but it can be done through the integration of independent sources of information (e.g., anatomy, archaeology, neurobiology, psychology), and validated with quantitative and experimental approaches. Read More

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December 2018

Prevalence and cardiac phenotype of patients with a phospholamban mutation.

Neth Heart J 2018 Dec 13. Epub 2018 Dec 13.

Department of Cardiology, University Medical Center Utrecht, Utrecht, The Netherlands.

Pathogenic mutations in the phospholamban (PLN) gene may give rise to inherited cardiomyopathies due to its role in calcium homeostasis. Several PLN mutations have been identified, with the R14del mutation being the most prevalent cardiomyopathy-related mutation in the Netherlands. It is present in patients diagnosed with arrhythmogenic cardiomyopathy as well as dilated cardiomyopathy. Read More

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December 2018
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Learning Differential Module Networks Across Multiple Experimental Conditions.

Methods Mol Biol 2019 ;1883:303-321

Division of Genetics and Genomics, The Roslin Institute, University of Edinburgh, Midlothian, Scotland, UK.

Module network inference is a statistical method to reconstruct gene regulatory networks, which uses probabilistic graphical models to learn modules of coregulated genes and their upstream regulatory programs from genome-wide gene expression and other omics data. Here, we review the basic theory of module network inference, present protocols for common gene regulatory network reconstruction scenarios based on the Lemon-Tree software, and show, using human gene expression data, how the software can also be applied to learn differential module networks across multiple experimental conditions. Read More

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January 2019

Gene Regulatory Network Inference: An Introductory Survey.

Methods Mol Biol 2019 ;1883:1-23

School of Informatics, University of Edinburgh, Edinburgh, UK.

Gene regulatory networks are powerful abstractions of biological systems. Since the advent of high-throughput measurement technologies in biology in the late 1990s, reconstructing the structure of such networks has been a central computational problem in systems biology. While the problem is certainly not solved in its entirety, considerable progress has been made in the last two decades, with mature tools now available. Read More

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January 2019

Eotaxins and C-C chemokine receptor type 3 in Parkinson's disease.

Acta Neurol Belg 2018 Dec 13. Epub 2018 Dec 13.

Geriatric Care Research Center, Rafsanjan University of Medical Science, Rafsanjan, Iran.

Parkinson's disease (PD) is one of the most common neuroinflammatory disorders and inflammatory processes seem to play an important role in the pathogenesis of PD. Chemokines as inflammatory mediators, which are involved in the recruitment of leukocytes, can play a role in the pathogenesis of PD. The aim of this study was to examine the serum level of eotaxins (CCL11, CCL24, and CCL26) and the expression of C-C chemokine receptor type 3 (CCR3) in patients with PD compared with healthy subjects. Read More

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December 2018

Optimizing the removal of nitrate from aqueous solutions via reduced graphite oxide-supported nZVI: synthesis, characterization, kinetics, and reduction mechanism.

Environ Sci Pollut Res Int 2018 Dec 13. Epub 2018 Dec 13.

Department of Civil and Environment Engineering, The Hong Kong Polytechnic University, Hong Kong, People's Republic of China.

Graphene has been considered an ideal absorbent and excellent carrier for nanoparticles. Reduced graphite oxide (rGO)-supported nanoscale zero-valent iron (nZVI@rGO) is an effective material for removing nitrate from water. nZVI@rGO nanocomposites were prepared by a liquid-phase reduction method and then applied for nitrate-nitrogen (NO-N) removal in aqueous solution under anaerobic conditions. Read More

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December 2018

Adolescent Depression and Substance Use: the Protective Role of Prosocial Peer Behavior.

J Abnorm Child Psychol 2018 Dec 14. Epub 2018 Dec 14.

University of Tennessee, Center for Behavioral Health Research, Henson Hall, 213, Knoxville, TN, 37996-3332, USA.

Adolescents with depression disorders have higher rates of substance use. In order to advance contextually relevant mental health interventions, basic research is needed to test social ecological mechanisms hypothesized to influence adolescent depression and substance use. Accordingly, we conducted growth curve modeling with a sample of 248 urban adolescents to determine if depression's effect on substance use was dependent upon peer network health (sum of peer risk and protective behaviors) and activity space risk (likelihood of high-risk behaviors at routine locations). Read More

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December 2018

Development of Novel Free Radical Initiated Peptide Sequencing Reagent: Application to Identification and Characterization of Peptides by Mass Spectrometry.

J Am Soc Mass Spectrom 2018 Dec 13. Epub 2018 Dec 13.

Department of Chemistry and Biochemistry, Center for Quantitative Obesity Research, Montclair State University, 1 Normal Avenue, Montclair, NJ, 07043, USA.

By incorporating a high proton affinity moiety to the charge localized free radical-initiated peptide sequencing (CL-FRIPS) reagent, FRIPS-MS technique has extended the applicability to hydrophobic peptides and peptides without basic amino acid residues (lysine, arginine, and histidine). Herein, the CL-FRIPS reagent has three moieties: (1) pyridine acting as the basic site to locate the proton, (2) 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO, a stable free radical) acting as the free radical precursor to generate the nascent free radical in the gas phase, and (3) N-hydroxysuccinimide (NHS) activated carboxylic acid acting as the coupling site to derivatize the N-terminus of peptides. The CL-FRIPS reagent allows for the characterization of peptides by generating sequencing ions, enzymatic cleavage-like radical-induced side chain losses, and the loss of TEMPO simultaneously via one-step collisional activation. Read More

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December 2018

Public housing and healthcare use: an investigation using linked administrative data.

Can J Public Health 2018 Dec 13. Epub 2018 Dec 13.

Department of Community Health Sciences, University of Manitoba, S113-750 Bannatyne Ave, Winnipeg, Manitoba, R3E 0W3, Canada.

Objective: This study investigated whether a move to public housing affects people's use of healthcare services.

Method: Using administrative data from Manitoba, the number of hospitalizations, general practitioner (GP), specialist and emergency department (ED) visits, and prescription drugs dispensed in the years before and after the housing move-in date (2012/2013) were measured for a public housing and matched cohort. Generalized linear models with generalized estimating equations tested for differences between the cohorts in utilization trends. Read More

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December 2018

A thermostable trypsin from freshwater fish Japanese dace (Tribolodon hakonensis): a comparison of the primary structures among fish trypsins.

Fish Physiol Biochem 2018 Dec 13. Epub 2018 Dec 13.

Laboratory of Marine Chemical Resource Development, Faculty of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido, 041-8611, Japan.

Trypsin from Japanese dace (Tribolodon hakonensis) (JD-T) living in freshwater (2-18 °C) was purified. JD-T represented typical fish trypsin characteristics regarding the effects of protease inhibitor, calcium-ion, and pH. For the effect of temperature, JD-T quite resembled to the trypsins from tropical-zone marine fish and freshwater fish (the catfish cultured in Thailand), i. Read More

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December 2018

A novel rare CUBN variant and three additional genes identified in Europeans with and without diabetes: results from an exome-wide association study of albuminuria.

Diabetologia 2018 Dec 13. Epub 2018 Dec 13.

Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.

Aims/hypothesis: Identifying rare coding variants associated with albuminuria may open new avenues for preventing chronic kidney disease and end-stage renal disease, which are highly prevalent in individuals with diabetes. Efforts to identify genetic susceptibility variants for albuminuria have so far been limited, with the majority of studies focusing on common variants.

Methods: We performed an exome-wide association study to identify coding variants in a two-stage (discovery and replication) approach. Read More

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December 2018

SIU-ICUD consultation on bladder cancer: basic science.

World J Urol 2018 Dec 13. Epub 2018 Dec 13.

Scott Department of Urology, Dan L. Duncan Cancer Center, Baylor College of Medicine, Houston, TX, USA.

Purpose: To provide a condensed summary of the Basic Science chapter that was included in the Third International Consultation on Bladder Cancer.

Methods: World bladder cancer basic science experts used the published literature to create summaries of recent progress in their areas of expertise.

Results: The completion of several large-scale genomics projects coupled with a strong collaborative culture within the research community and the exciting clinical activity of immune checkpoint blockade have combined to transform the bladder cancer research landscape. Read More

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December 2018

The effect of standing variants of the seated position on lumbar intersegmental angulation and spacing: a radiographic study of 20 asymptomatic subjects.

J Spine Surg 2018 Sep;4(3):509-515

US Department of Veterans Affairs, Northern California Health Care System, The David Grant USAF Medical Center, Fairfield, CA, USA.

Background: Pain while sitting is the primary complaint of many patients with lumbar spinal ailments, including those with discogenic low back pain and lumbar disc herniations. There has been little basic research on the different mechanical stresses that different sitting positions place on the spine. To demonstrate the effect of different sitting positions on lumbar intersegmental relationships. Read More

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September 2018
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Glacial changes in tropical climate amplified by the Indian Ocean.

Sci Adv 2018 Dec 12;4(12):eaat9658. Epub 2018 Dec 12.

National Center for Atmospheric Research, Climate and Global Dynamics Laboratory, 1850 Table Mesa Drive, Boulder, CO 80305, USA.

The mechanisms driving glacial-interglacial changes in the climate of the Indo-Pacific warm pool are poorly understood. Here, we address this question by combining paleoclimate proxies with model simulations of the Last Glacial Maximum climate. We find evidence of two mechanisms explaining key patterns of ocean cooling and rainfall change interpreted from proxy data. Read More

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December 2018

lncRNA MALAT1 binds chromatin remodeling subunit BRG1 to epigenetically promote inflammation-related hepatocellular carcinoma progression.

Oncoimmunology 2019 16;8(1):e1518628. Epub 2018 Oct 16.

National Key Laboratory of Medical Immunology & Institute of Immunology, Second Military Medical University, Shanghai China.

Hepatocellular carcinoma (HCC) is one type of cancers whose carcinogenesis and progression are closely related to chronic inflammation. Identifying the molecular mechanisms for inflammation-related HCC progression will contribute to improve the efficacy of current therapeutics for HCC patients. Many kinds of epigenetic factors, including long non-coding RNAs (lncRNAs), have been discovered to be important in HCC growth and metastasis. Read More

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October 2018

Tumor-infiltrating neutrophils predict therapeutic benefit of tyrosine kinase inhibitors in metastatic renal cell carcinoma.

Oncoimmunology 2019 2;8(1):e1515611. Epub 2018 Oct 2.

Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Fudan University, Shanghai, China.

Tumor-infiltrating neutrophils (TINs) show diverse predictive effects in the context of different cancer types and therapeutic regimens. In this study we investigated their relevance with therapeutic effect of tyrosine kinase inhibitors (TKIs) in metastatic renal cell carcinoma (mRCC). Two independent datasets including 271 mRCC patients treated by TKIs or IL-2/IFN-α based immunotherapy were retrospective included, and TINs were detected by immunohistochemistry. Read More

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October 2018
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Large-scale loyalty card data in health research.

Digit Health 2018 Jan-Dec;4:2055207618816898. Epub 2018 Nov 29.

Department of Food and Nutrition, University of Helsinki, Helsinki, Finland.

Objective: To study the characteristics of large-scale loyalty card data obtained in Finland, and to evaluate their potential and challenges in health research.

Methods: We contacted the holders of a certain loyalty card living in a specific region in Finland via email, and requested their electronic informed consent to obtain their basic background characteristics and grocery expenditure data from 2016 for health research purposes. Non-participation and the characteristics and expenditure of the participants were mainly analysed using summary statistics and figures. Read More

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November 2018
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Development of Fangjiomics for Systems Elucidation of Synergistic Mechanism Underlying Combination Therapy.

Comput Struct Biotechnol J 2018 20;16:565-572. Epub 2018 Nov 20.

Institute of Basic Research in Clinical Medicine, China Academy of Chinese Medical Sciences, Beijing 100700, China.

The rapid development of omics technology provides an opportunity for fulfilling the understanding of the synergistic mechanism of combination therapy. However, a systems theory to analyze synergy remains an ongoing challenge. Fangjiomics is a novel systems science based on a holistic theory integrated with reductionism which has been utilized to systematically elucidate the synergistic mechanisms underlying combination therapy using multi-target-, pathway- or network-based quantitative methods. Read More

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November 2018

Correlation between job security as motivation and career maturity among Japanese nursing students.

J Rural Med 2018 Nov 29;13(2):168-171. Epub 2018 Nov 29.

Department of Nursing, Faculty of Nursing, Kansai University of Nursing and Health Sciences, Japan.

For nursing students, career maturity -an individual's readiness to cope with career development tasks and make age-appropriate career decisions- is an essential factor influencing their desire to continue the job after qualifying as a nurse. Although job security as motivation to become a nurse might influence the career maturity of nursing students, it has not been demonstrated. In this study I aimed to elucidate the correlation between job security as motivation and career maturity among Japanese nursing students. Read More

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November 2018

Negative association of vegetable cultivation with the proportion of severely insufficient vegetable intake both directly and indirectly: A cross-sectional study in a city in Gunma, Japan.

J Rural Med 2018 Nov 29;13(2):160-167. Epub 2018 Nov 29.

Gunma University Graduate School of Health Sciences, Japan.

To clarify the relationship between the proportion of severely insufficient vegetable intake frequency and 1) vegetable cultivation, 2) frequency of receiving vegetable among non-cultivators. Residents aged 20 to 74 years in three areas of a city in Gunma Prefecture, Japan, were invited to participate. In September 2016, two sets of self-administered questionnaires were mailed to all 2,260 households in the three areas. Read More

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November 2018

The relationship between the houseboundedness and frailty of community-dwelling elderly persons.

J Rural Med 2018 Nov 29;13(2):141-150. Epub 2018 Nov 29.

Uji City, Kyoto Prefecture, Japan.

This study aimed to verify whether the incidence of frailty in elderly individuals is higher among those who are housebound than those who are not. This study found no correlation between elderly people's houseboundedeness and physical, mental, social, and overall frailty. However, the Tilburg Frailty Indicator (TFI) frailty score and grip strength value were higher in non-housebound elderly persons than in housebound elderly ones. Read More

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November 2018

Prognostic role of miR-760 in hepatocellular carcinoma.

Oncol Lett 2018 Dec 3;16(6):7239-7244. Epub 2018 Oct 3.

Department of General Surgery, Qilu Hospital of Shandong University, Jinan, Shandong 250012, P.R. China.

Previous studies have demonstrated that microRNA (miR)-760 serves an important role in various cancer types. However, to the best of our knowledge, its role in hepatocellular carcinoma (HCC) has not been fully elucidated. The current study investigated the prognostic role of miR-760 in HCC by using the Kaplan-Meier plotter database. Read More

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December 2018
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Differential expression of PMCA2 mRNA isoforms in a cohort of Spanish patients with breast tumor types.

Oncol Lett 2018 Dec 2;16(6):6950-6959. Epub 2018 Oct 2.

Department of Basic Biomedical Sciences, Faculty of Biomedical and Health Sciences, Universidad Europea de Madrid, Villaviciosa de Odón, Madrid 28670, Spain.

The present study examined the mRNA expression levels of different isoforms of the plasma membrane calcium ATPase 2 (PMCA2) gene generated by alternative splicing at the first intracellular loop (site A) and C-terminal region (site C) in 85 human breast cancer tumor and 69 adjacent non-tumor tissues. Associations were identified between the expression of PMCA2 splice isoforms and the following clinical variables: Estrogen receptor (ER), progesterone receptor (PR) and human epidermal growth factor receptor 2 (HER2) status, tumor size, staging and histological classification, and lymph node status. Transcripts including splice site A or splice site C were amplified by reverse transcription-quantitative polymerase chain reaction using PMCA2 isoform-specific primers. Read More

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December 2018
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Reconstruction of the Vancomycin-Susceptible Phenotype From a Vancomycin-Intermediate XN108.

Front Microbiol 2018 28;9:2955. Epub 2018 Nov 28.

Department of Microbiology, College of Basic Medical Sciences, Key Laboratory of Microbial Engineering Under the Educational Committee in Chongqing, Army Medical University (Third Military Medical University), Chongqing, China.

The emergence of vancomycin-intermediate (VISA) has raised healthcare concerns worldwide. VISA is often associated with multiple genetic changes. However, the relative contributions of these changes to VISA phenotypes are incompletely defined. Read More

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November 2018

Universal Stress Proteins Contribute Virulence in Catfish.

Front Microbiol 2018 28;9:2931. Epub 2018 Nov 28.

Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Starkville, MS, United States.

is an intracellular Gram-negative facultative pathogen causing enteric septicemia of catfish (ESC), a common disease resulting in substantial economic losses in the U.S. catfish industry. Read More

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November 2018

Predicting the Development of Normal-Appearing White Matter With Radiomics in the Aging Brain: A Longitudinal Clinical Study.

Front Aging Neurosci 2018 28;10:393. Epub 2018 Nov 28.

Department of Radiology, Zhejiang Provincial People's Hospital, Affiliated People's Hospital of Hangzhou Medical College, Hangzhou, China.

Normal-appearing white matter (NAWM) refers to the normal, yet diseased tissue around the white matter hyperintensities (WMH) on conventional MR images. Radiomics is an emerging quantitative imaging technique that provides more details than a traditional visual analysis. This study aims to explore whether WMH could be predicted during the early stages of NAWM, using a textural analysis in the general elderly population. Read More

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November 2018
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Rethinking Causation in Cancer with Evolutionary Developmental Biology.

Authors:
Katherine E Liu

Biol Theory 2018 27;13(4):228-242. Epub 2018 Jul 27.

Department of Ecology, Evolution, and Behavior, University of Minnesota, Saint Paul, MN USA.

Despite the productivity of basic cancer research, cancer continues to be a health burden to society because this research has not yielded corresponding clinical applications. Many proposed solutions to this dilemma have revolved around implementing organizational and policy changes related to cancer research. Here I argue for a different solution: a new conceptualization of causation in cancer. Read More

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Author Correction: Epigenetic regulation in psychiatric disorders.

Nat Rev Neurosci 2018 Dec 13. Epub 2018 Dec 13.

Department of Psychiatry and Center for Basic Neuroscience, The University of Texas Southwestern Medical Center, Dallas, Texas, USA.

In part c of Figure 1 in this article, the orientation of the tail of histone 3 with respect to the core region of this molecule was incorrect, and the amino acid residue K79 should not have been depicted in the tail. The corrected figure is shown below. The authors and editors thank T. Read More

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December 2018

Microenvironmental oxygen pressure orchestrates an anti- and pro-tumoral γδ T cell equilibrium via tumor-derived exosomes.

Oncogene 2018 Dec 13. Epub 2018 Dec 13.

Department of Head and Neck Surgery, Sichuan Cancer Hospital & Institute, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu, Sichuan, China.

γδ T cells are a unique lymphocyte population that have been reported to have either anti- or pro-tumoral functions in several cancer types, but the mechanisms are underinvestigated. Exosomes, initially considered to be cellular "garbage dumpsters," are now implicated in mediating interactions with the cellular environment. Hypoxia is a common feature of solid tumors and is believed to alter tumor-derived exosomes (TEXs), which mediate the hypoxic evolution of the tumor microenvironment in return. Read More

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December 2018

Epidemiological Characteristics of Atrial Fibrillation in Southern China: Results from the Guangzhou Heart Study.

Sci Rep 2018 Dec 13;8(1):17829. Epub 2018 Dec 13.

Guangdong Cardiovascular Institute, Guangdong General Hospital, Guangdong Academy of Medical Science, Guangzhou, 510080, China.

Precise prevalence of atrial fibrillation (AF) and the associated risk factors in southern China are rarely reported. This large population-based follow-up study, the Guangzhou Heart Study, was conducted from 2015 to 2017 to fill up this gap. Permanent residents aged 35 years and above in Guangzhou city were enrolled and demographic factors of participants were collected by a structured questionnaire. Read More

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December 2018

Genetics and genomics of pulmonary arterial hypertension.

Eur Respir J 2018 Dec 13. Epub 2018 Dec 13.

Vanderbilt University Medical Center, Nashville, TN, USA.

Since 2000 there have been major advances in our understanding of the genetic and genomics of pulmonary arterial hypertension (PAH), although there remains much to discover. Based on existing knowledge, around 25-30% of patients diagnosed with idiopathic PAH have an underlying Mendelian genetic cause for their condition and should be classified as heritable PAH (HPAH). Here, we summarise the known genetic and genomic drivers of PAH, the insights these provide into pathobiology, and the opportunities afforded for development of novel therapeutic approaches. Read More

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December 2018

A Golgi-released subpopulation of the trans-Golgi network mediates protein secretion in Arabidopsis.

Plant Physiol 2018 Dec 13. Epub 2018 Dec 13.

RIKEN CITY: Saitama POSTAL_CODE: 351-0198 Japan [JP].

Spatiotemporal coordination of protein trafficking between organelles is essential for eukaryotic cells, and the post-Golgi interface, including the trans-Golgi network (TGN), is a pivotal hub for multiple trafficking pathways. The Golgi-released independent TGN (GI-TGN) is a compartment described only in plant cells, and its cellular and physiological roles remain elusive. In Arabidopsis thaliana, the SYP4 group Qa-SNARE (soluble N-ethyl-maleimide sensitive factor attachment protein receptor) membrane fusion proteins are shared components of TGN and GI-TGN and regulate secretory and vacuolar transport. Read More

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December 2018

Identification of fluorinated (R)-(-)-aporphine derivatives as potent and selective ligands at serotonin 5-HT receptor.

Bioorg Med Chem Lett 2018 Nov 23. Epub 2018 Nov 23.

Division of Basic Neuroscience, Medicinal Chemistry Program, McLean Hospital, Belmont, MA 02478, United States; Department of Psychiatry, Harvard Medical School, Boston, MA 02115, United States.

A series of novel aporphine derivatives were synthesized for initial screening at the 5-HT receptor subtypes. Among them, Compounds 11a and 11b were identified as potent 5-HT hit ligands with high selectivity over other 5-HT receptor subtypes. Molecular docking study revealed that compounds 11a and 11b formed two key interactions with the binding site of 5-HT receptor, including a salt-bridge to D3. Read More

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November 2018

Characterization by high-resolution crystal structure analysis of a triple-helix region of human collagen type III with potent cell adhesion activity.

Biochem Biophys Res Commun 2018 Dec 10. Epub 2018 Dec 10.

Key Laboratory of Medical Molecular Virology of MOE/MOH, School of Basic Medical Sciences and Shanghai Public Health Clinical Center, Fudan-Jinbo Joint Research Center, Fudan University, Shanghai, 200302, China. Electronic address:

Collagen is one of the most abundant and important proteins in the human body. Human collagen type III (hCOL3A1) belongs to the fibril-forming collagens and is widely distributed in extensible connective tissue like skin, internal organs, or the vascular system. It plays key roles in wound healing, collagen fibrillogenesis, and normal cardiovascular development in human. Read More

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December 2018

Biomimetic regulation of dentine remineralization by amino acid in vitro.

Dent Mater 2018 Dec 10. Epub 2018 Dec 10.

The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, 237# Luoyu Road, Wuhan 430079, China. Electronic address:

Objective: The aim of this study was to evaluate the effect of conditioning solutions containing DL-aspartic amino (Asp) on dentine remineralization induced by bioactive glass 45S5 (BAG) in a simulated oral environment.

Methods: Sixty dentine discs from human third molars were used. Dentine specimens were treated with ethylene diamine tetraacetic acid (EDTA) to create a partially demineralization model and randomly divided to 4 groups: Artificial saliva (AS) group, Asp group (pretreated with Asp and remineralized with distilled water), BAG group (pretreated with distilled water and remineralized by BAG), Asp-BAG group (pretreated with Asp and remineralized by BAG). Read More

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December 2018

Comprehensive analysis of oxidative stress markers and antioxidants status in preeclampsia.

Taiwan J Obstet Gynecol 2018 Dec;57(6):779-790

Department of Medical Biotechnology, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran.

Preeclampsia is a hypertensive disorder in pregnant women, which can be the leading cause of maternal and neonatal death or premature birth. Although the cause of preeclampsia is still not clear, local or systemic oxidative stress may explain the pathological features associated with this complication. However, it is not clear whether oxidative stress is the cause or the result of preeclampsia. Read More

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December 2018

Morphology, performance and attachment function in Corynosoma spp. (Acanthocephala).

Parasit Vectors 2018 Dec 13;11(1):633. Epub 2018 Dec 13.

Instituto Cavanilles de Biodiversidad y Biología Evolutiva, Parque Científico, Universidad de Valencia, Catedrático José Beltrán 2, 46980, Paterna, Valencia, España.

Background: Functional inference on the attachment of acanthocephalans has generally been drawn directly from morphology. However, performance of structures is often non-intuitive and context-dependent, thus performance analysis should be included whenever possible to improve functional interpretation. In acanthocephalans, performance analysis of attachment is available only for Acanthocephalus ranae, a species that solely relies on the proboscis to attach. Read More

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December 2018

Long-term soil metal exposure impaired temporal variation in microbial metatranscriptomes and enriched active phages.

Microbiome 2018 Dec 13;6(1):223. Epub 2018 Dec 13.

Section of Microbiology, University of Copenhagen, Universitetsparken 15, 2100, Copenhagen, Denmark.

Background: It remains unclear whether adaptation and changes in diversity associated to a long-term perturbation are sufficient to ensure functional resilience of soil microbial communities. We used RNA-based approaches (16S rRNA gene transcript amplicon coupled to shotgun mRNA sequencing) to study the legacy effects of a century-long soil copper (Cu) pollution on microbial activity and composition, as well as its effect on the capacity of the microbial community to react to temporal fluctuations.

Results: Despite evidence of microbial adaptation (e. Read More

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December 2018

Current state and future directions of technology-based ecological momentary assessments and interventions for major depressive disorder: protocol for a systematic review.

Syst Rev 2018 Dec 13;7(1):233. Epub 2018 Dec 13.

Department of Basic Psychology, Clinic and Psychobiology, Universitat Jaume I, Av. Sos Baynat, s/n, 12071, Castellón, Spain.

Background: Ecological momentary assessments (EMAs) and ecological momentary interventions (EMIs) represent a novel approach for the assessment and delivery of psychological support to depressed patients in daily life. Beyond the classical paper-and-pencil daily diaries, the more recent progresses in Information and Communication Technologies (ICT) enabled researchers to bring all the needed processes together in only one device, i.e. Read More

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December 2018

The combination of bFGF and CHIR99021 maintains stable self-renewal of mouse adult retinal progenitor cells.

Stem Cell Res Ther 2018 Dec 13;9(1):346. Epub 2018 Dec 13.

Department of Ophthalmology of Shanghai Tenth People's Hospital, and Tongji Eye Institute, Tongji University School of Medicine, Shanghai, 200072, China.

Background: Millions of people are affected with retinal diseases that eventually cause blindness, and retinal progenitor cell (RPC) transplantation is a promising therapeutic avenue. However, RPC expansion and the underlying regulation mechanisms remain elusive.

Methods: Adult mouse neural RPCs (mNRPCs) were isolated and amplified with the combination of basic fibroblast growth factor (bFGF) and glycogen synthase kinase 3 (GSK3) inhibitor CHIR99021. Read More

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December 2018

Gut microbiota dysbiosis in male patients with chronic traumatic complete spinal cord injury.

J Transl Med 2018 Dec 13;16(1):353. Epub 2018 Dec 13.

School of Rehabilitation Medicine, Capital Medical University, No. 10 Jiaomen North Road, Fengtai District, Beijing, 100068, China.

Background: Neurogenic bowel dysfunction (NBD) is a major physical and psychological problem in patients with spinal cord injury (SCI), and gut dysbiosis is commonly occurs in SCI. Here, we document neurogenic bowel management of male patients with chronic traumatic complete SCI in our centre and perform comparative analysis of the gut microbiota between our patients and healthy males.

Methods: A total of 43 male patients with chronic traumatic complete SCI (20 with quadriplegia and 23 with paraplegia) and 23 healthy male adults were enrolled. Read More

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December 2018
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Diversity of Rickettsia species in border regions of northwestern China.

Parasit Vectors 2018 Dec 13;11(1):634. Epub 2018 Dec 13.

Department of Basic Medicine, School of Medicine, Shihezi University, Shihezi, Xinjiang, Uygur Autonomous Region, China.

Background: Rickettsia species belonging to the spotted fever group (SFG) cause infections in humans, domestic animals and wildlife. At least ten SFG Rickettsia species are known to occur in China. However, the distribution of rickettsiae in ticks and fleas in the border region of northwestern China have not been systematically studied to date. Read More

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December 2018

The changing landscape of phase II/III metastatic sarcoma clinical trials-analysis of ClinicalTrials.gov.

BMC Cancer 2018 Dec 13;18(1):1251. Epub 2018 Dec 13.

Department of Medical Melanoma and Sarcoma, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, 651 East Dongfeng Road, Guangzhou, 510060, China.

Background: Well-designed clinical trials are of great importance in validating novel treatments and ensuring an evidence-based approach for sarcoma. This study aimed to provide a comprehensive landscape of the characteristics of metastatic or advanced sarcoma clinical trials using the substantial resource of the ClincialTrials.gov database. Read More

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December 2018

Retinal fluid changes and therapeutic effects in symptomatic circumscribed choroidal hemangioma patients: a long-term follow up study.

BMC Ophthalmol 2018 Dec 13;18(1):321. Epub 2018 Dec 13.

Department of Ophthalmology, Eye and ENT Hospital, Severance Hospital, Institute of Vision Research, Yonsei University College of Medicine, 50-1 Yonsei-ro, Seodaemun-gu, Seoul, 03722, South Korea.

Background: Changes in retinal fluid patterns associated with circumscribed choroidal hemangioma (CCH) have not been investigated yet. A long-term follow-up study was performed to evaluate the changes of retinal fluid patterns and treatment responses.

Methods: We retrospectively reviewed medical records of all CCH patients diagnosed between November 2005 and March 2017. Read More

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December 2018

Probiotics Streptococcus salivarius 24SMB and Streptococcus oralis 89a interfere with biofilm formation of pathogens of the upper respiratory tract.

BMC Infect Dis 2018 Dec 13;18(1):653. Epub 2018 Dec 13.

Laboratory of Clinical Chemistry and Microbiology, IRCCS Orthopedic Institute Galeazzi, Milan, Italy.

Background: Infections of the ears, paranasal sinuses, nose and throat are very common and represent a serious issue for the healthcare system. Bacterial biofilms have been linked to upper respiratory tract infections and antibiotic resistance, raising serious concerns regarding the therapeutic management of such infections. In this context, novel strategies able to fight biofilms may be therapeutically beneficial and offer a valid alternative to conventional antimicrobials. Read More

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December 2018