430,941 results match your criteria Basic Excisional Surgery


Effect modification of FADS2 polymorphisms on the association between breastfeeding and intelligence: results from a collaborative meta-analysis.

Int J Epidemiol 2018 Dec 11. Epub 2018 Dec 11.

Population Health Sciences, University of Bristol, Bristol, UK.

Background: Accumulating evidence suggests that breastfeeding benefits children's intelligence, possibly due to long-chain polyunsaturated fatty acids (LC-PUFAs) present in breast milk. Under a nutritional adequacy hypothesis, an interaction between breastfeeding and genetic variants associated with endogenous LC-PUFAs synthesis might be expected. However, the literature on this topic is controversial. Read More

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December 2018
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Silencing the ACAT1 Gene in Human SH-SY5Y Neuroblastoma Cells Inhibits the Expression of Cyclo-Oxygenase 2 (COX2) and Reduces β-Amyloid-Induced Toxicity Due to Activation of Protein Kinase C (PKC) and ERK.

Med Sci Monit 2018 Dec 12;24:9007-9018. Epub 2018 Dec 12.

Department of Anatomy, Histology and Embryology, School of Basic Medical Sciences, Fudan University, Shanghai, China (mainland).

BACKGROUND Acyl-coenzymeA: cholesterol acyltransferase (ACAT) 1, a key enzyme converting excess free cholesterol to cholesterol esters, has been demonstrated to be associated with the pathogenesis of Alzheimer disease (AD). However, the mechanism underlying the protective role of ACAT1 blockage in AD progression remains elusive. MATERIAL AND METHODS Human neuroblastoma SH-SY5Y cells were treated for 24 h with increasing concentrations of aggregated Aβ₂₅₋₃₅ (5, 15, 25, and 45 μmol) with or without the ACAT1 siRNA pretreatment. Read More

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December 2018

Conformational stability and dynamics of the cancer-associated isoform Δ133p53β are modulated by p53 peptides and p53-specific DNA.

FASEB J 2018 Dec 12:fj201801973R. Epub 2018 Dec 12.

Basic Science Program, Leidos Biomedical Research, Inc., Cancer and Inflammation Program, National Cancer Institute, Frederick, Maryland, USA; and.

p53 is a tumor suppressor protein that maintains genome stability, but its Δ133p53β and Δ160p53β isoforms promote breast cancer cell invasion. The sequence truncations in the p53 core domain raise key questions related to their physicochemical properties, including structural stabilities, interaction mechanisms, and DNA-binding abilities. Herein, we investigated the conformational dynamics of Δ133p53β and Δ160p53β with and without binding to p53-specific DNA by using molecular dynamics simulations. Read More

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December 2018

Penetration of new antidiabetic medications in East Asian countries and the United States: A cross-national comparative study.

PLoS One 2018 12;13(12):e0208796. Epub 2018 Dec 12.

Duke Clinical Research Institute, Duke University School of Medicine, Durham, North Carolina, United States of America.

Background: The number of patients with diabetes is increasing particularly in Asia-Pacific region. Many of them are treated with antidiabetics. As the basis of the studies on the benefit and harm of antidiabetic drugs in the region, the information on patterns of market penetration of new classes of antidiabetic medications is important in providing context for subsequent research and analyzing and interpreting results. Read More

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December 2018

New skeletal material sheds light on the palaeobiology of the Pleistocene marsupial carnivore, Thylacoleo carnifex.

PLoS One 2018 12;13(12):e0208020. Epub 2018 Dec 12.

Ecology and Evolution, College of Science and Engineering, Flinders University, Adelaide, South Australia, Australia.

The extinct marsupial 'lion' Thylacoleo carnifex was Australia's largest mammalian carnivore. Despite being the topic of more discussion than any other extinct Australian marsupial (excepting perhaps the Thylacine), basic aspects of its palaeobiology, including its locomotory repertoire, remain poorly understood. Recent discoveries allowed the first reconstruction of an entire skeleton including the first complete tail and hitherto-unrecognised clavicles. Read More

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December 2018

Screen for modulators of atonal homolog 1 gene expression using notch pathway-relevant gene transcription based cellular assays.

PLoS One 2018 12;13(12):e0207140. Epub 2018 Dec 12.

R&D Alternative Discovery and Development, GlaxoSmithKline, Stevenage, Hertfordshire, United Kingdom.

Atonal homolog 1 (Atoh1) is a basic helix-loop-helix 9 (bHLH) transcription factor acting downstream of Notch and is required for the differentiation of sensory hair cells in the inner ear and the specification of secretory cells during the intestinal crypt cell regeneration. Motivated by the observations that the upregulation of Atoh1 gene expression, through genetic manipulation or pharmacological inhibition of Notch signaling (e.g. Read More

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December 2018

[Good practices and ethical principles: as necessary as ever].

Authors:
Lely Solari

Rev Gastroenterol Peru 2018 Jul-Sep;38(3):306-309

Escuela de Medicina, Universidad Peruana de Ciencias Aplicadas. Lima, Perú.

We are going through a moment in which acts of disregard to the ethics in the public function are being disclosed at all levels. It is important to know the codes of ethics and good practices of each of the functions performed. In the field of scientific publications, it is highly recommended that all researchers who wish to publish their studies get to know the good scientific practices and the basic ethical principles of publications in science. Read More

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December 2018

Assessing the Value of Endoscopy Simulator Modules Designed to Prepare Residents for the Fundamentals of Endoscopic Surgery Examination.

Dis Colon Rectum 2018 Nov 28. Epub 2018 Nov 28.

Background: The Fundamentals of Endoscopic Surgery examination is required for all general surgery residents. The test modules are not available for practice before the examination; however, similar modules are commercially available.

Objective: This study aims to determine which modules are most valuable for resident training and preparation for the examination by evaluating which correlates best with experience level. Read More

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November 2018

Measuring Work Related Physical and Mental Health Function: Updating the Work Disability Functional Assessment Battery (WD-FAB) using Item Response Theory.

J Occup Environ Med 2018 Dec 11. Epub 2018 Dec 11.

Tufts University, Department of Occupational Therapy, Medford, MA (Marfeo); University of Pittsburgh, School of Health and Rehabilitation Sciences, Department of Physical Therapy (McDonough); Boston University School of Public Health, Department of Health Law Policy and Management, Boston, MA (Ni, Peterik, Kazis); National Institutes of Health Rehabilitation Medicine Department, Bethesda, MD (Porcino, Rasch, Chan); VHA Office of Reporting, Analytics, Performance, Improvement and Deployment (RAPID) (Meterko).

Objective: To further improve measurement of work-related physical and mental health by updating the Work Disability Assessment Battery (WD-FAB).

Methods: Cross-sectional study with 1024 disability claimants and 1000 working age (21-66 years) adults in the United States. Developed new items to replenish the WD-FAB and analyzed using factor analysis and item response theory (IRT). Read More

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December 2018

Engaging in the prevention of pressure injuries in spinal cord injury: A qualitative study of community-dwelling individuals' different styles of prevention in Switzerland.

J Spinal Cord Med 2018 Dec 12:1-10. Epub 2018 Dec 12.

a Department of Health Sciences and Health Policy , University of Lucerne and Swiss Paraplegic Research , Lucerne/Nottwil , Switzerland.

Context: Spinal cord injury (SCI) is a complex chronic condition with multiple self-management requirements and a high prevalence of complications. Pressure injuries (PIs) are among the most common ones and represent a frequent reason for re-hospitalization. This study aimed to identify styles of prevention that individuals with SCI adopt to deal with the risk of developing PIs. Read More

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December 2018

Co-infection organises epidemiological networks of viruses and hosts and reveals hubs of transmission.

Phytopathology 2018 Dec 12. Epub 2018 Dec 12.

Universidad Politecnica de Madrid, Centro de Biotecnología y Genómica de Plantas, Madrid, Madrid, Spain ;

Multiple virus infections affect the competence of host plants to transmit disease. The effects of co-infection on transmission are expected to produce ecologically complex pathogen and host-pathogen interactions. However, the prediction of disease risk will rely on untangling non-random from random patterns of infection to identify underlying processes that drive these interactions. Read More

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December 2018

pH-Responsive Pickering Foams Generated by Surfactant-Free Soft Hydrogel Particles.

Langmuir 2018 Dec 12. Epub 2018 Dec 12.

Pickering foams are foams stabilized by particles and are generally known to have a good stability. A special sub-class of particle stabilized foams is the stimuli-responsive Pickering foams that can be formed or de-constructed by applying an external stimuli or changing the environmental conditions; such intelligent particles could find use in many practical applications. Here, we synthesized the surfactant-free biocompatible poly[2(diethylamino)ethylmethacrylate] (PDEAEMA) hydrogel particles (HGPs) by emulsion polymerization. Read More

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December 2018
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Housefly (Musca domestica) larvae powder, preventing oxidative stress injury via regulation of UCP4 and CyclinD1 and modulation of JNK and P38 signaling in APP/PS1 mice.

Food Funct 2018 Dec 12. Epub 2018 Dec 12.

School of Basic Courses, Guangzhou, Guangdong 510006, China and Guangdong Key Laboratory of Pharmaceutical Bioactive Substances, Guangzhou, Guangdong 510006, China and Department of Biochemistry and Molecular Biology, Guangdong Pharmaceutical University, Guangzhou Higher Education Mega Center, Guangzhou, Guangdong 510006, China.

Housefly (Musca domestica) Larvae powder (HL) is rich in antioxidants. As oxidative stress is considered as one of the main pathogenesis in Alzheimer's Disease (AD), this study was designed to explore the protective effects of HL as an antioxidant on APP/PS1 mice. 2-Month-old APP/PS1 mice were divided into a model control (MC) group, a Donepezil group and a HL group, and C57BL/6 mice were used as the normal control (NC) group. Read More

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December 2018

Acutely infected teeth: to extract or not to extract?

Braz Oral Res 2018 Dec 6;32:e124. Epub 2018 Dec 6.

Necmettin Erbakan University, Faculty of Dentistry, Oral and Maxillofacial Surgery Department, Konya, Turkey.

Not only laymen but also dentists generally believe that extraction of acutely infected teeth should be avoided until the infection subdues by using systemic antibiotics. The aim of this study was to compare perioperative complications in routine extractions of acutely infected teeth with extractions of asymptomatic teeth. This prospective study was performed with 82 patients. Read More

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December 2018

Nursing professionals' knowledge regarding the management of waste produced in primary health care.

Rev Bras Enferm 2018 ;71(suppl 6):2728-2734

Universidade Federal do Piauí, Department of Nursing. Teresina, Piauí, Brazil.

Objective: To evaluate the knowledge of Nursing professionals regarding waste management in primary healthcare services.

Method: Descriptive study with a mixed approach, developed with 42 nursing professionals who worked in basic health units of a capital in the Brazilian Northeast Region. Quantitative variables were analyzed by the SPSS 20. Read More

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January 2018

Construction and validation of a distance Basic Life Support Course.

Rev Bras Enferm 2018 ;71(suppl 6):2698-2705

Universidade Federal do Rio Grande do Norte. Natal, Rio Grande do Norte, Brazil.

Objective: to describe the content construction and validation process of the Distance Education Basic Life Support Course.

Method: methodological study, developed through literature review, outlined in the light of the Bloom's Taxonomy and Ausubel's Meaningful Learning Theory. For validation, the analysis was performed with judges, using a structured tool. Read More

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January 2018

CAN VIRTUAL REALITY BE AS GOOD AS OPERATING ROOM TRAINING? EXPERIENCE FROM A RESIDENCY PROGRAM IN GENERAL SURGERY.

Arq Bras Cir Dig 2018 Dec 6;31(4):e1397. Epub 2018 Dec 6.

Department of Surgery, Hospital de Clínicas de Porto Alegre, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.

Background: The increasingly intense usage of technology applied to videosurgery and the advent of robotic platforms accelerated the use of virtual models in training surgical skills.

Aim: To evaluate the performance of a general surgery department's residents in a video-simulated laparoscopic cholecystectomy in order to understand whether training with virtual reality is sufficient to provide the skills that are normally acquired in hands-on experience at the operating room.

Methods: An observational study with twenty-five first- and second-year general surgery residents. Read More

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December 2018
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rs2217560 was Associated with Pulmonary Arterial Hypertension in Systemic Lupus Erythematosus.

Chin Med J (Engl) 2018 Dec;131(24):3020-3021

Department of Rheumatology, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Science, Key Laboratory of Rheumatology and Clinical Immunology, Ministry of Education, Beijing 100730, China.

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December 2018

Role of dalteparin sodium on the growth of cancer cells and tumor-associated angiogenesis in A549 human lung cancer cell line and grafted mouse model.

J Cancer Res Ther 2018 Dec;14(Supplement):S985-S992

Department of Respiration, The First Affiliated Hospital of Bengbu Medical College, Bengbu, China.

Purpose: To investigate the effects of dalteparin sodium on the expression of vascular endothelial growth factor (VEGF), VEGF receptor (VEGFR), and hypoxia-inducible factor 1α (HIF-1α) in A549 human lung cancer (LC) cell line and a human A549-grafted nude mouse model.

Materials And Methods: A549 human lung adenocarcinoma cell line was divided into control group, treated using normal saline (NS); and dalteparin sodium groups, receiving 5, 15, 50, and 150 IU/ml of dalteparin sodium, respectively. Human A549-grafted nude mouse was induced through subcutaneous (SC) injection of A549 (5 × 10/0. Read More

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December 2018

Neuroprotective effect of Notch pathway inhibitor DAPT against focal cerebral ischemia/reperfusion 3 hours before model establishment.

Neural Regen Res 2019 Mar;14(3):452-461

Department of Anatomy, School of Basic Medicine, Guizhou Medical University, Guian New District, Guizhou Province, China.

As an inhibitor of the Notch signaling pathway, N-[N-(3,5-difluorohenacetyl)-l-alanyl]-S-phenylglycine tert-butyl ester (DAPT) may protect brain tissue from serious ischemic injury. This study aimed to explore neuroprotection by DAPT after cerebral ischemia/reperfusion (I/R) injury. DAPT was intraperitoneally injected 3 hours before the establishment of a focal cerebral I/R model in the right middle cerebral artery of obstructed mice. Read More

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Multitarget therapeutic strategies for Alzheimer's disease.

Neural Regen Res 2019 Mar;14(3):437-440

Department of Medicinal Chemistry, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt; Department of Chemistry, University of Iowa, Iowa City, IA, USA.

Neurodegenerative diseases such as Alzheimer's, Huntington's and Parkinson's diseases have multifaceted nature because of the different factors contributing to their progression. The complex nature of neurodegenerative diseases has developed a pressing need to design multitarget-directed ligands to address the complementary pathways involved in these diseases. The major enzyme targets for development of therapeutics for Alzheimer's disease are cholinesterase and β-secretase enzymes. Read More

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The tolerant immune system: Biological significance and clinical implications of T cell tolerance.

Endocr Metab Immune Disord Drug Targets 2018 Dec 11. Epub 2018 Dec 11.

Department of Basic Medical Sciences, Neuroscience and Sensory Organs, School of Medicine, University of Bari, Bari. Italy.

Background: T cell tolerance both at thymic and peripheral levels is a mechanism of protection finalized to eradicate autoreactive T cell clones and/or to maintain immune homeostasis, especially, postnatally. Central tolerance occurs in the thymic medulla via a mechanism of negative selection which leads to the eradication of autoreactive T cell clones. Mechanisms of action: Such a tolerogenic event relies on Fas-mediated apoptosis of autoreactive T cell clones operated by thymic dendritic cells (DCs), on the one hand. Read More

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December 2018
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The Plasminogen Activation System Promotes Neurorepair in the Ischemic Brain.

Authors:
Manuel Yepes

Curr Drug Targets 2018 Dec 11. Epub 2018 Dec 11.

Division of Neuropharmacology and Neurologic Diseases, Yerkes National Primate Research Center; Atlanta, GA. United States.

The plasminogen activation (PA) system was originally thought to exclusively promote the degradation of fibrin by catalyzing the conversion of plasminogen into plasmin via two serine proteinases: tissue-type plasminogen activator (tPA) and urokinase-type plasminogen activator (uPA). However, experimental evidence accumulated over the last 30 years indicates that tPA and uPA are also found in the central nervous system (CNS), where they have a plethora of functions that not always require plasmin generation or fibrin degradation. For example, plasminogen-dependent and -independent effects of tPA and uPA play a central role in the pathophysiological events that underlie one of the leading causes of mortality and disability in the world: cerebral ischemia. Read More

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December 2018
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Assessment of the Safety of the New Hybrid Pencil Type Energy (NP) Device Used Close to the Recurrent Laryngeal Nerve in a Porcine Model: Comparison With a Conventional Electrosurgical Knife.

Surg Innov 2018 Dec 12:1553350618817646. Epub 2018 Dec 12.

1 Keio University, School of Medicine Tokyo, Japan.

Background: Recurrent laryngeal nerve (RLN) paralysis is mainly associated with esophagectomy, and it may result in not only other morbidities, such as aspiration pneumonia, but also in long-term issues. Therefore, an approach to prevent RLN paralysis is necessary. The present study was designed to determine the technical usability of the new hybrid pencil type energy (NP) device developed by Olympus Corporation (Tokyo, Japan) and compare it with a conventional electrosurgical knife (EK) for resection around the RLN lymph nodes. Read More

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December 2018

Determination of CRP Autoantibodies by SPR Immunoassay.

Methods Mol Biol 2019 ;1901:205-219

MOE Key Laboratory of Environment and Genes Related to Diseases, School of Basic Medical Sciences, Xi'an Jiaotong University, Xi'an, Shanxi, People's Republic of China.

Biosensors based on the principle of surface plasmon resonance (SPR) are surface-sensitive optical devices used for monitoring biomolecular interactions at the sensor surface in real time without any labeling. It is used in a wide variety of areas including proteomics, clinical diagnosis, environmental monitoring, drug discovery, and food analysis. C-reactive protein (CRP) is a marker of inflammation, which undergoes conformation changes in local lesions, leading to the formation of mCRP. Read More

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January 2019

Overview of Basic Immunology and Translational Relevance for Clinical Investigators.

Adv Exp Med Biol 2018 ;995:1-41

Baylor College of Medicine and Texas Children's Hospital, Houston, TX, USA.

Tumor exists as a complex network of structures with an ability to evolve and evade the host immune surveillance mechanism. The immune milieu which includes macrophages, dendritic cells, natural killer cells, neutrophils, mast cells, B cells, and T cells are found in the core, the invasive margin, or the adjacent stromal or lymphoid component of the tumor. The immune infiltrate is heterogeneous and varies within a patient and between patients of the same tumor histology. Read More

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January 2018

Phage Display Technology for Human Monoclonal Antibodies.

Methods Mol Biol 2019 ;1904:319-338

Department of Life Sciences, University of Trieste, Trieste, Italy.

During the last 20 years in vitro technologies opened powerful routes to combine the generation of large libraries together with fast selection and screening procedures to identify lead candidates. One of the most successful methods is based on the use of filamentous phages. Functional Antibodies (Abs) fragments can be displayed on the surface of phages by fusing the coding sequence of the antibody variable (V) regions to the phage minor coat protein pIII. Read More

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January 2019

Basic Procedures for Detection and Cytotoxicity of Chimeric Antigen Receptors.

Methods Mol Biol 2019 ;1904:299-306

Department of Cardiac Research, Cumballa Hill Hospital and Heart Institute, Mumbai, India.

Chimeric antigen receptors against CD19 (anti-CD19-CAR) are widely recognized and used by not only researchers associated with immunology, molecular biology, and cell biology but also physicians to treat B-cell malignancies. Anti-CD19-CAR is currently clinically available as one of the therapeutic modalities for refractory acute B-cell-typed lymphoblastic leukemia (B-ALL) patients. However, to detect CAR on the cell surface and investigate the efficacy of CAR-T cells, there are numerous experimental modalities including flow cytometry, the Cr-releasing assay, immunoblot, and immunostaining. Read More

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January 2019

Humanization and Simultaneous Optimization of Monoclonal Antibody.

Methods Mol Biol 2019 ;1904:213-230

Research Division, Chugai Pharmaceutical, Kamakura, Kanagawa, Japan.

Antibody humanization is an essential technology for reducing the potential risk of immunogenicity associated with animal-derived antibodies and has been applied to a majority of the therapeutic antibodies on the market. For developing an antibody molecule as a pharmaceutical at the current biotechnology level, however, other properties also have to be considered in parallel with humanization in antibody generation and optimization. This section describes the critical properties of therapeutic antibodies that should be sufficiently qualified, including immunogenicity, binding affinity, physicochemical stability, expression in host cells and pharmacokinetics, and the basic methodologies of antibody engineering involved. Read More

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January 2019

An Efficient Method to Generate Monoclonal Antibodies from Human B Cells.

Methods Mol Biol 2019 ;1904:109-145

Department of Medicine, Section of Rheumatology, The Knapp Center for Lupus and Immunology, University of Chicago, Chicago, IL, USA.

In the age of personalized medicine, an efficient method to generate monoclonal antibodies (mAbs) is essential for biomedical and immunotherapeutic research. Numerous aspects of basic B-cell biology can be studied at the monoclonal level, including B-cell development, antibody responses to infection or vaccination, and autoimmune responses. Single-cell B-cell receptor cloning allows for the rapid generation of antigen-specific mAbs in a matter of several weeks. Read More

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January 2019

An Introduction to the Performance of Immunohistochemistry.

Methods Mol Biol 2019 ;1897:289-298

Division of Neuropathology, Department of Pathology and Laboratory Medicine, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA.

Immunohistochemistry (IHC) is a powerful technique that exploits the specific binding between an antibody and antigen to detect and localize specific antigens in cells and tissue, most commonly detected and examined with the light microscope. A standard tool in many fields in the research setting, IHC has become an essential ancillary technique in clinical diagnostics in anatomic pathology (Lin F, Chen Z. Arch Pathol Lab Med 138:1564-1577, 2014) with the advent of antigen retrieval methods allowing it to be performed conveniently on formalin fixed paraffin embedded (FFPE) tissue (Taylor CR, Shi S-R, Barr NJ. Read More

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January 2019

Making Formalin-Fixed, Paraffin Embedded Blocks.

Methods Mol Biol 2019 ;1897:253-268

Department of Pathology, Iran University of Medical Sciences, Tehran, Tehran Province, Iran.

Paraffin embedding is a standard technique used in clinical and research laboratories to create a formalin-fixed, paraffin-embedded (FFPE) block of tissue. Formalin-fixed tissue undergoes tissue processing and then is embedded in paraffin (wax) to create a FFPE block or paraffin block. The paraffin block can be cut using a microtome to generate thin sections of tissue contained in paraffin to be stained or paraffin tissue ribbons suitable for nucleic acid extraction. Read More

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January 2019

Laboratory Safety: Chemical and Physical Hazards.

Methods Mol Biol 2019 ;1897:243-252

Department of Pathology (Neuropathology) and Laboratory Medicine, University Hospital-London Health Sciences Center, London, ON, Canada.

Chemical and physical hazards are part of the daily routine when working in biorepositories. This chapter provides a brief overview of some common physical and chemical hazards encountered in biorepositories, with some basic principles to help eliminate and control these hazards by implementing safe work practices. Read More

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January 2019

Procurement and Preservation of Plants.

Methods Mol Biol 2019 ;1897:191-212

Departments of Pathology and Laboratory Medicine, University of California, San Francisco, San Francisco, CA, USA.

Plants have been used by humans for thousands of years for a great variety of purposes. While many people collect plants as a hobby, specimen collections also contribute to the broader scientific community. To provide the greatest value, the specimen must be collected and documented properly. Read More

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January 2019

Collection and Preservation of Terrestrial Arthropods.

Methods Mol Biol 2019 ;1897:163-189

Department of Entomology, University of California, Riverside, Riverside, CA, USA.

Arthropods comprise an amazingly diverse group of life forms that are extensively studied in almost every field of the biological sciences. Given that the vast majority of animals are arthropods (primarily insects, arachnids, and crustaceans), knowledge of the specific methods to collect and preserves these organisms for scientific purposes can be indispensable. The application of this research can play major roles in fundamental aspects of human society, including agriculture and medicine. Read More

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January 2019

Orientation and Training of New Biobank Personnel.

Methods Mol Biol 2019 ;1897:51-63

Division of Neuropathology, Department of Pathology and Laboratory Medicine, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA.

The personnel who operate a biomedical biobank should function as a unit to efficiently manage the numerous types of biospecimens that are to be utilized for both clinical and research purposes. Therefore, new staff must be appropriately trained before becoming fully integrated into the work environment. This chapter focuses on several key aspects to this training that should be completed by all personnel. Read More

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January 2019
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An Introduction to Hardware, Software, and Other Information Technology Needs of Biomedical Biobanks.

Methods Mol Biol 2019 ;1897:17-29

Brain Tumor Translational Resource, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA.

Biobanks support medical research by facilitating access to biospecimens. Biospecimens that are linked to clinical and molecular information are particularly useful for translational biomedical research. Tracking and managing the biospecimens and their associated data are therefore crucial tasks in the functioning of a biobank. Read More

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January 2019

Sports Injuries: Diagnosis, Prevention, Stem Cell Therapy, and Medical Sport Strategy.

Adv Exp Med Biol 2018 Dec 12. Epub 2018 Dec 12.

Cell Therapy and Regenerative Medicine Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran.

Sports injuries diagnosis, prevention, and treatment are the most important issues of sports medicine. Fortunately, sports injuries are often treated effectively, and people with damage recover and return to the sport in a satisfactory condition. Meanwhile, many sports injuries and complications can be prevented. Read More

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December 2018
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Generation and Characterization of Fibroblast-Specific Basigin Knockout Mice.

Mol Biotechnol 2018 Dec 11. Epub 2018 Dec 11.

Department of Cell Biology, National Translational Science Center for Molecular Medicine, State Key Laboratory of Cancer Biology, Fourth Military Medical University, No. 169 Changle West Road, Xi'an, 710032, China.

Basigin is a well-known extracellular stimulator of fibroblasts and may confer resistance to apoptosis of fibroblasts in vitro under some pathological status, but its exact function in fibroblasts and the underlying mechanism remain poorly understood. The systematic Basigin gene knockout leads to the perinatal lethality of mice, which limits the delineation of its function in vivo. In this study, we generated a fibroblast-specific Basigin knock-out mouse model and demonstrated the successful deletion of Basigin in fibroblasts. Read More

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December 2018

TGF-β1 Restores Hippocampal Synaptic Plasticity and Memory in Alzheimer Model via the PI3K/Akt/Wnt/β-Catenin Signaling Pathway.

J Mol Neurosci 2018 Dec 12. Epub 2018 Dec 12.

Department of Neurology, the First Affiliated Hospital of Guangxi University of Chinese Medicine, No. 89-9 Dongge Road, Nanning, 530023, Guangxi, China.

Alzheimer's disease (AD) is the most common neurodegenerative disturbances. Dysfunction of synaptic plasticity and decline in cognitive functions are the most prominent features of AD, but the mechanisms of pathogenesis have not been well elucidated. In this paper, transforming growth factor-β1 (TGF-β1) was found to be reduced in the hippocampus of AD mouse which was accompanied by impaired pine density, synaptic plasticity, and memory function. Read More

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December 2018

The nucleolar transcriptome regulates Piwi shuttling between the nucleolus and the nucleoplasm.

Chromosome Res 2018 Dec 12. Epub 2018 Dec 12.

Department of Molecular Genetics of the Cell, Institute of Molecular Genetics, Russian Academy of Sciences, 2 Kurchatov Sq., Moscow, Russian Federation, 123182.

The nucleolus contains a lot of proteins unrelated to ribosome biogenesis. Some of these proteins shuttle between the nucleolus and the nucleoplasm regulating the cell cycle and stress response. The piRNA binding protein Piwi is involved in silencing of transposable elements (TEs) in the Drosophila gonads. Read More

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December 2018

Developmental toxicity and apoptosis in zebrafish embryos induced by low-dose γ-ray irradiation.

Environ Sci Pollut Res Int 2018 Dec 12. Epub 2018 Dec 12.

Key Discipline Laboratory for National Defense for Biotechnology in Uranium Mining and Hydrometallurgy, University of South China, Hengyang, 421001, China.

In this paper, the developmental toxicity and apoptosis in zebrafish (Danio rerio) embryos induced by 0.01, 0.05, and 0. Read More

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December 2018

Invasive breast cancers in adolescent and young adult women show more aggressive immunohistochemical and clinical features than those in women aged 40-44 years.

Breast Cancer 2018 Dec 11. Epub 2018 Dec 11.

Department of Breast and Medical Oncology, National Cancer Center Hospital, Tokyo 5-1-1 Tsukiji, Chuo-ku, Tokyo, 104-0045, Japan.

Background: Limited knowledge exists concerning the clinicopathological features of breast cancers (BCs) occurring in adolescent and young adult (AYA) women. We evaluated tumor characteristics in AYA women in comparison with those in middle-aged premenopausal women.

Methods: From consecutive AYA patients (< 35-year-old) with invasive BC in a single institute, 82 patients first treated with surgery were examined. Read More

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December 2018

Xylanosomes produced by Cellulosimicrobium cellulans F16 were diverse in size, but resembled in subunit composition.

Arch Microbiol 2018 Dec 11. Epub 2018 Dec 11.

College of Life Science, Dalian Minzu University, Dalian, 116600, China.

The hemicellulolytic enzyme system produced by Cellulosimicrobium cellulans strain F16 was resolved by ultracentrifugation and size exclusion chromatography. The particle size and molecular weight were determined by both dynamic light scattering and negative stain electron microscopy. The results showed that xylanosomes produced by strain F16 were found to have an apparent sedimentation coefficient of 28 S, were diverse in size (18-70 nm), molecular weight (11-78 MDa) and morphology, but resembled in subunit composition (SDS-PAGE and proteomic results). Read More

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December 2018

Pathogenicity of two novel human-origin H7N9 highly pathogenic avian influenza viruses in chickens and ducks.

Arch Virol 2018 Dec 11. Epub 2018 Dec 11.

Division of Transboundary Animal Disease, National Institute of Animal Health, National Agriculture and Food Research Organization (NARO), 3-1-5 Kannondai, Tsukuba, Ibaraki, 305-0856, Japan.

Human infection by low-pathogenic avian influenza viruses of the H7N9 subtype was first reported in March 2013 in China. Subsequently, these viruses caused five outbreaks through September 2017. In the fifth outbreak, H7N9 virus possessing a multiple basic amino acid insertion in the cleavage site of hemagglutinin emerged and caused 4% of all human infections in that period. Read More

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December 2018
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Assessing Illinois Residents' Support for Natural Recolonization of Apex Predators.

Environ Manage 2018 Dec 11. Epub 2018 Dec 11.

Illinois Natural History Survey, University of Illinois at Urbana-Champaign, Urbana, IL, USA.

Understanding sources of difference in public attitudes toward wildlife is critical for the design of effective policy instruments. In this article we explored the role of wildlife value orientations and stakeholder group membership (general public versus agricultural producers) in shaping residents support for the natural recolonization of apex predators (black bear, cougar, gray wolf), in Illinois, USA. Results demonstrate differences in attitudes toward recolonization as a function of residents' basic beliefs about the human-wildlife relationship and stakeholder group membership. Read More

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December 2018

Morphological evaluation of the calcaneofibular ligament in different ankle positions using a three-dimensional MRI sequence.

Surg Radiol Anat 2018 Dec 11. Epub 2018 Dec 11.

Department of Orthopaedic Surgery, Sapporo Medical University School of Medicine, Sapporo, Hokkaido, 060-8543, Japan.

Purpose: Evaluating images of the lateral ligament of the ankle is not easy, and evaluation of the calcaneofibular ligament (CFL) in particular is difficult. We prospectively conducted morphological measurements of the CFL in different ankle positions and obtain basic data for use in functional assessment of the CFL, diagnosis of CFL injury, and determination of treatment effects.

Methods: The subjects were ten healthy volunteers (ten ankles) with a mean age of 27. Read More

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December 2018

Simultaneous extraction of acidic and basic drugs via on-chip electromembrane extraction using a single-compartment microfluidic device.

Analyst 2018 Dec 12. Epub 2018 Dec 12.

Faculty of Food Industry and Agriculture, Department of Food Science & Technology, Standard Research Institute (SRI), Karaj, P.O. Box 31745-139, Iran.

In this study, a new chip was designed for simultaneous extraction of acidic and basic drugs by a single chamber on-chip electromembrane extraction (CEME) followed by high performance liquid chromatography. Diclofenac (DIC) and nalmefene (NAL) were selected as acidic and basic model analytes, respectively. In this device, simultaneous extraction of the analytes was carried out using a single compartment. Read More

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December 2018

Differentially expressed serum host proteins in hepatitis B and C viral infections.

Virusdisease 2018 Dec 1;29(4):468-477. Epub 2018 Sep 1.

1Transfusion Transmitted Diseases Department, National Institute of Immunohaematology, 13th floor, New Multi-storeyed Bldg, KEM Hospital Campus, Parel, Mumbai, 400 012 India.

Hepatitis B virus (HBV) and Hepatitis C virus (HCV) infection often lead to hepatocellular carcinoma (HCC), which is mostly detected in advanced stage. Hence, its early detection is of paramount importance using a biomarker having sensitivity and specificity both. The present study highlights differentially expressed host proteins in response to HBV/HCV infection at different stages. Read More

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December 2018