298,006 results match your criteria Basal Cell Carcinoma


Mitochondrial Respiration in Outer Retina Contributes to Light-Evoked Increase in Hydration In Vivo.

Invest Ophthalmol Vis Sci 2018 Dec;59(15):5957-5964

Department of Ophthalmology, Visual and Anatomical Sciences, Wayne State University School of Medicine, Detroit, Michigan, United States.

Purpose: To test the hypothesis that mitochondrial respiration contributes to local changes in hydration involved in phototransduction-driven expansion of outer retina, as measured by structural responses on optical coherence tomography (OCT) and diffusion magnetic resonance imaging (MRI).

Methods: Oxygen consumption rate and mitochondrial reserve capacity of freshly isolated C57BL/6 and 129S6/SvEvTac mouse retina were measured using a Seahorse Extracellular Flux Analyzer. Light-stimulated outer retina layer water content was determined by proton density MRI, structure and thickness by ultrahigh-resolution OCT, and water mobility by diffusion MRI. Read More

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December 2018

Synaptic potentiation and rapid antidepressant response to ketamine in treatment-resistant major depression: A replication study.

Psychiatry Res Neuroimaging 2018 Sep 12;283:64-66. Epub 2018 Sep 12.

Experimental Therapeutics and Pathophysiology Branch, National Institute of Mental Health, National Institutes of Health, Bethesda, Maryland, USA.

Preclinical and clinical evidence has demonstrated that ketamine has rapid antidepressant effects. Studies using pre-treatment with an AMPA inhibitor suggest that enhancing AMPA throughput is crucial to ketamine's effects, including increases in both basal and evoked gamma power. This study sought to replicate previous findings of increased gamma response to a somatosensory stimulus at 230 min and Day 1 in ketamine responders versus non-responders in 31 depressed subjects and 25 healthy controls. Read More

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September 2018

Neutral/negative α-AR antagonists and calcium channel blockers at comparison in functional tests on guinea-pig smooth muscle and myocardium.

Pharmacol Rep 2018 Oct 11;71(1):128-132. Epub 2018 Oct 11.

Dipartimento di Farmacia e Biotecnologie, Università degli Studi di Bologna, Bologna, Italy. Electronic address:

Background: Constitutive (agonist-independent) activity is a prerogative of many G protein-coupled receptors (GPCRs) including α-adrenoceptors (α-ARs). Inhibition of such an activity at α-AR subtypes by antagonists with negative efficacy is difficult to be adequately tested.

Methods: In the present experimental approach, we compared the activity of three calcium channel blockers (nifedipine, diltiazem and verapamil) and of three potent benzodioxane-based α-AR antagonists, differing for subtype selectivity and inverse agonist properties, in producing smooth muscle relaxation and negative inotropy under the same test conditions. Read More

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October 2018

A randomized controlled trial to test efficacy of digital enhancements of storybooks in support of narrative comprehension and word learning.

J Exp Child Psychol 2018 Dec 11;179:212-226. Epub 2018 Dec 11.

Institute of Education, ELTE Eötvös Loránd University, 1053 Budapest, Hungary; Department of Language, Literature and Communication, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, the Netherlands; Faculty of Arts and Education, University of Stavanger, 4036 Stavanger, Norway.

Experiments with film-like story presentations have been found to be beneficial in supporting children's story comprehension and word learning. The main goal of the current study was to disentangle the effects of visual and auditory enhancements in digital books. Participants were 99 typically developing children (41 boys and 58 girls) aged 4-6 years from two public kindergartens in Bursa, Turkey. Read More

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December 2018

TRPC channels mediated calcium entry is required for proliferation of human airway smooth muscle cells induced by nicotine-nAChR.

Biochimie 2018 Dec 11. Epub 2018 Dec 11.

Respiratory Medicine, Hunan Provincial People's Hospital, No. 61 Jiefang Xi Road, Changsha, 410219, Hunan, PR China; Institute of Respiratory Medicine, Changsha Medical College, No. 1501 Leifeng Road, Changsha, 410219, Hunan, PR China. Electronic address:

The present study was designed to explore the role of transient receptor potential canonical 3 (TRPC3) in nicotine-induced chronic obstructive pulmonary disease (COPD) and its underlying mechanism. In this study, the expression and localization of α5 nicotinic acetylcholine receptor (α5-nAchR) in lung tissues were determined by western blotting and immunohistochemistry. The quantitative real-time PCR (qRT-PCR) analysis was performed to examine the mRNA expression levels of α5-nAchR and TRPC3 in human airway smooth muscle cells (HASMCs). Read More

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December 2018

Differences in the Upslope of the Precordial Body Surface ECG T-Wave Reflect Right To Left Dispersion of Repolarization in the Intact Human Heart.

Heart Rhythm 2018 Dec 11. Epub 2018 Dec 11.

Department of Cardiac Electrophysiology, The Barts Heart Center, St Bartholomew's Hospital, London, UK; Institute of Cardiovascular Science, University College London, London, UK.

Background: The relationship between the surface ECG T-wave to intracardiac repolarization is poorly understood.

Objectives: This study aimed to examine the association between intracardiac ventricular repolarization and the T-wave on the body surface ECG (SECG).

Methods: 10 normal heart patients (age 35 +/- 15yrs, 6Male), were studied. Read More

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December 2018

Modulation of vitamin D signaling by the pioneer factor CEBPA.

Biochim Biophys Acta Gene Regul Mech 2018 Dec 11. Epub 2018 Dec 11.

School of Medicine, Institute of Biomedicine, University of Eastern Finland, FI-70211 Kuopio, Finland. Electronic address:

The myeloid master regulator CCAAT enhancer-binding protein alpha (CEBPA) is known as a pioneer factor. In this study, we report the CEBPA cistrome of THP-1 human monocytes after stimulation with the vitamin D receptor (VDR) ligand 1α,25-dihydroxyvitamin D (1,25(OH)D) for 2, 8 and 24 h. About a third of the genomic VDR binding sites co-located with those of CEBPA. Read More

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December 2018

Serum inhibin B concentration as a predictor of age at first menstruation in girls with idiopathic central precocious puberty.

PLoS One 2018 14;13(12):e0205810. Epub 2018 Dec 14.

Fondation Ophtalmologique Adolphe de Rothschild and Université Paris Descartes, Paris, France.

Objective: To compare the serum inhibin B, anti-Müllerian hormone (AMH) and leptin concentrations in girls with idiopathic central precocious puberty (CPP) to their concomitant characteristics and evaluate the capacity of each of these hormones to predict the age at first menstruation in those who were untreated and who completed their puberty.

Methods: This single-center study included 94 girls selected from a cohort of 493 girls seen between 1981 and 2012 and diagnosed with idiopathic CPP for whom a remaining serum sample collected at the initial evaluation was available. Of these 25 were untreated and completed their puberty. Read More

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December 2018

The urothelial cell line UROtsa transformed by arsenite and cadmium display basal characteristics associated with muscle invasive urothelial cancers.

PLoS One 2018 14;13(12):e0207877. Epub 2018 Dec 14.

Department of Pathology, School of Medicine and Health Sciences, University of North Dakota, Grand Forks, North Dakota, United States of America.

Muscle invasive urothelial carcinomas are divided into various molecular subtypes with basal and luminal subtypes being the prominent ones. The basal muscle-invasive urothelial carcinomas are generally more aggressive at presentation and significantly enriched with squamous features. Our laboratory has developed an in-vitro model of urothelial cancer by transforming the immortalized cell line UROtsa with arsenite (As3+) and cadmium (Cd2+). Read More

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December 2018

NO FACE-DOWN POSITIONING SURGERY FOR THE REPAIR OF CHRONIC IDIOPATHIC MACULAR HOLES.

Retina 2018 Dec 6. Epub 2018 Dec 6.

Department of Ophthalmology, Mayo Clinic, Rochester, Minnesota.

Purpose: To report visual and anatomical outcomes after the repair of chronic idiopathic macular holes (MHs) with no face-down positioning.

Methods: We conducted a retrospective review of chronic MH cases of greater than 1-year duration that were repaired through pars plana vitrectomy with broad internal limiting membrane peeling and no face-down positioning between March 2009 and December 2017. There were 18 eyes of 18 patients that met inclusion criteria. Read More

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December 2018

Impact of dose capping in insulin glargine/lixisenatide fixed-ratio combination trials in patients with type 2 diabetes.

Curr Med Res Opin 2018 Dec 14:1-21. Epub 2018 Dec 14.

d National Research Institute , Los Angeles , CA , USA .

Objective: The LixiLan clinical trials of insulin glargine (iGlar)/lixisenatide fixed-ratio combination (iGlarLixi) investigated the safety and efficacy of iGlarLixi versus iGlar: LixiLan-O (NCT02058147) in patients with type 2 diabetes (T2D) inadequately controlled on oral antidiabetes drugs (OADs), and LixiLan-L (NCT02058160) in patients with T2D inadequately controlled on basal insulin ± OADs. In these two trials, both iGlar and iGlarLixi were titrated to a maximum (capped) dose of 60 units. We evaluated whether this may have affected the reported glycemic efficacy of iGlar, and the glycemic differences observed between treatment with iGlarLixi and iGlar. Read More

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December 2018

Injecting RNA interference lentiviruses targeting the muscarinic 3 receptor gene into the bladder wall inhibits neurogenic detrusor overactivity in rats with spinal cord injury.

Neurourol Urodyn 2018 Dec 14. Epub 2018 Dec 14.

Department of Urology, Xuanwu Hospital Capital Medical University, Xuanwu District, Beijing, China.

Aims: To investigate the effects of injecting RNA interference (RNAi) lentiviruses targeting the muscarinic 3 (M ) receptor gene into the bladder wall on bladder activity in rats with spinal cord injury (SCI).

Methods: Four M RNAi lentiviruses were constructed and used to infect primary cultured bladder smooth muscle cells (BSMCs). Western blotting and quantitative reverse transcription polymerase chain reaction (qRT-PCR) were performed to determine the optimal RNAi lentivirus with the highest interference efficiency. Read More

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December 2018

Effects of dietary ramie powder at various levels on growth performance, antioxidative capacity and fatty acid profile of finishing pigs.

J Anim Physiol Anim Nutr (Berl) 2018 Dec 13. Epub 2018 Dec 13.

Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production, Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Changsha, Hunan, China.

This study was conducted to evaluate the effects of ramie (Boehmeria nivea, previously known as a fibre crop and also called "China grass") included in diets on growth performance, antioxidative capacity and muscular fatty acid profile of finishing pigs. A total of 180 Xiangcun Black pigs (initial body weight =70.71 ± 1. Read More

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December 2018

Sublaminar organization of the human subplate: developmental changes in the distribution of neurons, glia, growing axons and extracellular matrix.

J Anat 2018 Dec 13. Epub 2018 Dec 13.

Croatian Institute for Brain Research, School of Medicine, University of Zagreb, Zagreb, Croatia.

The objective of this paper was to collect normative data essential for analyzing the subplate (SP) role in pathogenesis of developmental disorders, characterized by abnormal circuitry, such as hypoxic-ischemic lesions, autism and schizophrenia. The main cytological features of the SP, such as low cell density, early differentiation of neurons and glia, plexiform arrangement of axons and dendrites, presence of synapses and a large amount of extracellular matrix (ECM) distinguish this compartment from the cell-dense cortical plate (CP; towards pia) and large fiber bundles of external axonal strata of fetal white matter (towards ventricle). For SP delineation from these adjacent layers based on combined cytological criteria, we analyzed the sublaminar distribution of different microstructural elements and the associated maturational gradients throughout development, using immunocytochemical and histological techniques on postmortem brain material (Zagreb Neuroembryological Collection). Read More

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December 2018

Direct and indirect effects of climate on richness drive the latitudinal diversity gradient in forest trees.

Ecol Lett 2018 Dec 12. Epub 2018 Dec 12.

ECNU-Alberta Joint Lab for Biodiversity Study, Tiantong National Station for Forest Ecosystem Research, East China Normal University, 200241, Shanghai.

Climate is widely recognised as an important determinant of the latitudinal diversity gradient. However, most existing studies make no distinction between direct and indirect effects of climate, which substantially hinders our understanding of how climate constrains biodiversity globally. Using data from 35 large forest plots, we test hypothesised relationships amongst climate, topography, forest structural attributes (stem abundance, tree size variation and stand basal area) and tree species richness to better understand drivers of latitudinal tree diversity patterns. Read More

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December 2018

Interatrial septal fat thickness and left atrial stiffness are mechanistic links between nonalcoholic fatty liver disease and incident atrial fibrillation.

Echocardiography 2018 Dec 12. Epub 2018 Dec 12.

Cardiology Department, Zagazig University Hospital-Egypt, Zagazig, Egypt.

Background And Aim: Recently, a clear evidence suggests that nonalcoholic fatty liver disease (NAFLD) is associated with an increased risk of incident atrial fibrillation (AF). Yet, the underlying pathogenesis is speculative. Thereby, we aimed to investigate the hypothesis that, interatrial thickness (IAST) and left atrial stiffness (LASt) might have mechanistic links between NAFLD and AF. Read More

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December 2018

Clinical and molecular characteristics of bladder urothelial carcinoma subtypes.

J Cell Biochem 2018 Dec 12. Epub 2018 Dec 12.

Department of Organization, General Hospital of Benxi Iron and Steel Co., Benxi, China.

Bladder urothelial carcinoma (BLCA) is a common malignancy with high heterogeneity. A reasonable molecular subtyping can facilitate biological study and personalized therapy of BLCA. In this study, unsupervised consensus clustering was used to acquire the molecular subtypes of BLCA based on messenger RNA (mRNA) and microRNA (miRNA) data. Read More

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December 2018
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Bile salt (glycochenodeoxycholate acid) induces cell survival and chemoresistance in hepatocellular carcinoma.

J Cell Physiol 2018 Dec 12. Epub 2018 Dec 12.

Key Laboratory of Nanobiological Technology of Chinese Ministry of Health, Xiangya Hospital, Central South University, Changsha, Hunan, People's Republic of China.

Objective: Glycochenodeoxycholate acid (GCDA) is a toxic component in bile salts. It plays an important role in the development and progression of liver cancer. In this study, we investigated the underlying mechanism of GCDA in hepatocarcinogenesis and chemotherapy resistance. Read More

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December 2018

Active Edge Site-Rich Carbon Nanocatalysts with Enhanced Electron Transfer for Efficient Electrochemical Hydrogen Peroxide Production.

Angew Chem Int Ed Engl 2018 Dec 10. Epub 2018 Dec 10.

Ulsan National Institute of Science and Technology (UNIST), Department of Energy Engineering and School of Energy and Chemical Engineering, 50 UNIST-gil, 44919, Ulsan, KOREA, REPUBLIC OF.

Electrochemical hydrogen peroxide production enables clean and on-site production of H2O2, for which highly active and selective electrocatalysts are of crucial importance. Here, we report a highly efficient, metal-free carbon nanocatalyst that possesses abundant active, oxygenated graphitic edge sites. The edge site-rich nanocarbon catalyst exhibits ~22 times higher activity for H2O2 production than a basal plane-rich carbon nanotube with a H2O2 selectivity over 90%. Read More

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December 2018

Morin inhibits PDGF-induced proliferation, migration, and invasion of vascular smooth muscle cells via modulating p27KIP1, AKT, and MMP-9 activities.

Gen Physiol Biophys 2018 Sep;37(6):633-645

Department of Food Science and Nutrition, Jeju National University, Jeju 63243, South Korea.

Hyper-proliferation and migration of vascular smooth muscle cells (VSMCs) are closely associated with atherosclerosis. Recently, the flavonol morin has been reported to exhibit potent anti-oxidant and anti-inflammatory activities. Therefore, we investigated molecular mechanisms of morin in VSMCs stimulated by PDGF. Read More

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September 2018

Basal ganglia hemorrhage in a case report following spinal surgery.

BMC Neurol 2018 Dec 14;18(1):204. Epub 2018 Dec 14.

Neurosurgery and Radiology, Neurology Department, University of Minnesota, Minneapolis, MN, USA.

Background: Intracranial hemorrhage is a rare but potentially severe complication of spinal surgery. Most reported post-operative ICH cases consist of cerebellar hemorrhage. There are fewer reported cases of supratentorial ICH following spinal surgery. Read More

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December 2018

Piezoelectric Effects in Surface-Engineered Two-Dimensional Group III Nitrides.

ACS Appl Mater Interfaces 2018 Dec 14. Epub 2018 Dec 14.

Piezoelectric effects of two-dimensional (2D) group III-V compounds have received considered attention in recent years because of their wide applications in semiconductor devices. However, it faces a problem that only metastable or unstable structures are non-centrosymmetric with piezoelectricity, thus leading to the difficulty in experimental observation. Motivated by the recent advances in the synthesis of 2D group III nitrides, in this paper, for the first time, we study the piezoelectric properties of the 2D group III nitrides (XN, X = Al, Ga, and In) with buckled hexagonal configurations by surface passivation, which are thermodynamically stable. Read More

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December 2018

Combination of asystole and sinus arrest with junctional escape rhythm seen in Takotsubo cardiomyopathy: A case report.

J Cardiol Cases 2013 Oct 31;8(4):125-128. Epub 2013 Aug 31.

Clinical Cardiology, Maimonides Medical Center, Brooklyn, NY, USA.

Takotsubo cardiomyopathy (TCM) is characterized by a transient depression of contractile function of mid and/or apical segments of the left ventricle with compensatory hyperkinesis of the basal segment. It leads to ballooning of the apex in systole, resembling an octopus trap, hence the name. There have been many different arrhythmias associated with TCM. Read More

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October 2013

A new variation of dual left anterior descending coronary artery.

J Cardiol Cases 2016 Jul 31;14(1):26-28. Epub 2016 Mar 31.

Division of Cardiovascular Health and Disease, Department of Internal Medicine University of Cincinnati, Cincinnati, OH, USA.

A 63-year-old male with past medical history of type II diabetes mellitus, hypertension, hyperlipidemia, stroke, and permanent pacemaker implant for poor chronotropic response to exercise underwent coronary computed tomography angiography (CCTA) for worsening atypical chest pain. The patient had normal myocardial perfusion by nuclear stress testing 3 months prior to this test. A rare variation of dual left anterior descending coronary artery (LAD) was identified by CCTA and subsequent coronary angiography confirmed a dual LAD and revealed no significant stenosis of the coronary arteries. Read More

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A case of Takotsubo cardiomyopathy that was confirmed by cardiac catheterization at all three times of onset.

J Cardiol Cases 2015 Nov 15;12(5):152-155. Epub 2015 Jul 15.

Department of Cardiology, Cardiovascular Center, Toranomon Hospital, Tokyo, Japan.

Takotsubo cardiomyopathy (TTC) is a cardiomyopathy associated with emotional and physiological stress which can be recurrent. We report a case of recurrent TTC (total 3 times) induced by emotional stress, which was diagnosed accurately by coronary angiography (CAG) and left ventriculography (LVG). A 77-year-old female was referred to our institute because of stomach ache, nausea, dizziness, and vomiting. Read More

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November 2015

The demography of the lion-tailed macaque (Macaca silenus) in rain forest fragments in the Anamalai Hills, South India.

Primates 2000 Apr;41(2):119-126

Salim Ali Centre for Ornithology and Natural History, Anaikatty P.O., 641 108, Coimbatore, India.

We examined demographic consequences of habitat fragmentation in the lion-tailed macaque in the Anamalai Hills in southern Western Ghats. The parameters examined were group size, age/sex composition, and birth rate, in relation to various habitat parameters. Demographic parameters were estimated for 11 groups in 8 rain forest fragments, during January to May 1996. Read More

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Coronary spasm as a cause of takotsubo cardiomyopathy and intraventricular obstruction.

J Cardiol Cases 2010 Oct 24;2(2):e83-e87. Epub 2010 May 24.

Kumamoto Saishunsou National Hospital, 2659 Suya, Kohshi 861-1196, Kumamoto, Japan.

A 79-year-old man presented to the emergency room because of chest pain on 3 successive mornings. An electrocardiogram (ECG) showed ST segment elevation in leads II, III, and aVF. Laboratory findings including cardiac enzymes, were within normal limits, except a positive result for the troponin T test. Read More

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October 2010

Recurrent takotsubo cardiomyopathy with variant forms of left ventricular dysfunction.

J Cardiol Cases 2010 Aug 20;2(1):e37-e40. Epub 2010 Feb 20.

Ohtaki Hearts Clinic, Bunkyo-ku, Tokyo, Japan.

A 78-year-old man presented to our emergency department with dyspnea. The patient was diagnosed as having pneumonia from the chest X-ray which depicted mass-like opacity in the left lower lobe. On the 5th hospital day, electrocardiography showed giant negative T waves in pericardial leads and echocardiography demonstrated left ventricular apical akinesis and basal hyperkinesis. Read More

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Investigating the Association Between Baseline Characteristics (HbA1c and Body Mass Index) and Clinical Outcomes of Fast-Acting Insulin Aspart in People with Diabetes: A Post Hoc Analysis.

Diabetes Ther 2018 Dec 13. Epub 2018 Dec 13.

Clinical and Experimental Endocrinology, University Hospital Leuven, Catholic University of Leuven, Leuven, Belgium.

Introduction: The aim of this study was to investigate the association between baseline characteristics [HbA1c and body mass index (BMI)] and the effect of mealtime fast-acting insulin aspart (faster aspart) relative to insulin aspart (IAsp) or basal-only insulin therapy on several efficacy and safety outcomes in people with diabetes.

Methods: Post hoc analysis of three randomised phase 3a trials in people with type 1 diabetes (T1D; onset 1) and type 2 diabetes (T2D; onset 2 and 3). Participants (N = 1686) were stratified according to baseline BMI (< 25 kg/m, 25-< 30 kg/m, ≥ 30 kg/m) or HbA1c (≤ 58 mmol/mol, > 58-< 64 mmol/mol, ≥ 64 mmol/mol; ≤ 7. Read More

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December 2018
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Isolation of living apical and basal cell lineages of early proembryos for transcriptome analysis.

Plant Reprod 2018 Dec 13. Epub 2018 Dec 13.

State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, 430072, China.

Plant zygotes usually undergo asymmetrical cell division, giving rise to the formation of two daughter cells with distinct developmental cell fate. The small apical cell will develop into the major part of embryo proper, whereas the larger basal cell will divide to form a transient suspensor. Thus, the apical and basal cell lineages are an excellent model to study cell fate determination in relation to zygote polarity. Read More

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December 2018

Modifiable Risk-factors for Keratinocyte Cancers in Australia: A Case-control Study.

Acta Derm Venereol 2018 Dec 13. Epub 2018 Dec 13.

Department of Clinical Epidemiology and Applied Biostatistics, University Hospital Tübingen, DE-72076 Tübingen, Germany.

Keratinocyte cancer (KC) is the most common malignancy in Caucasians. The incidence of KC has increased over the past 40 years. This case-control study investigated the environmental and host risk-factors responsible for development of KC in Australia's high-risk population. Read More

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December 2018

A Case of Mistaken Identity: Glutaric Aciduria Type I Masquerading as Postmeningitic Hydrocephalus.

J Clin Imaging Sci 2018 15;8:50. Epub 2018 Nov 15.

Department of Radiology and Imaging, Vardhman Mahavir Medical College and Safdarjung Hospital, New Delhi, India.

We report the characteristic neuroimaging features of a rare metabolic leukodystrophy in an 8-year-old boy, born of consanguineous parenthood. The child presented with macrocrania, regression of milestones, and dystonia. The patient was referred for magnetic resonance imaging with a clinical diagnosis of postmeningitic hydrocephalus. Read More

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November 2018

Air pollutant particulate matter 2.5 induces dry eye syndrome in mice.

Sci Rep 2018 Dec 13;8(1):17828. Epub 2018 Dec 13.

Department of Ophthalmology, the First Affiliated Hospital of Nanchang University, Nanchang, 330006, Jiangxi Province, China.

In this study, we explored the effects of particulate matter 2.5 (PM) eye drops on the ocular surface structure and tear function in mice and established a novel animal model for dry eye research. We found that, following treatment with PM, the tear volume and, the tear film break-up time showed statistical differences at each time point (P < 0. Read More

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December 2018

Identification of a PH domain-containing protein which is localized to crystalloid bodies of Plasmodium ookinetes.

Malar J 2018 Dec 13;17(1):466. Epub 2018 Dec 13.

Division of Molecular Parasitology, Proteo-Science Center, Ehime University, Toon, Ehime, 791-0295, Japan.

Background: For the success of the malaria control and eradication programme it is essential to reduce parasite transmission by mosquito vectors. In the midguts of mosquitoes fed with parasite-infected blood, sexual-stage parasites fertilize to develop into motile ookinetes that traverse midgut epithelial cells and reside adjacent the basal lamina. Therefore, the ookinete is a promising target of transmission-blocking vaccines to break the parasite lifecycle in mosquito vectors. Read More

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December 2018

Novel features in the structure of P-glycoprotein (ABCB1) in the post-hydrolytic state as determined at 7.9 Å resolution.

BMC Struct Biol 2018 Dec 13;18(1):17. Epub 2018 Dec 13.

School of Biology, Faculty of Biology Medicine and Health, Michael Smith Building, The University of Manchester, Oxford Road, Manchester, M13 9PL, UK.

Background: P-glycoprotein (ABCB1) is an ATP-binding cassette transporter that plays an important role in the clearance of drugs and xenobiotics and is associated with multi-drug resistance in cancer. Although several P-glycoprotein structures are available, these are either at low resolution, or represent mutated and/or quiescent states of the protein.

Results: In the post-hydrolytic state the structure of the wild-type protein has been resolved at about 8 Å resolution. Read More

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December 2018

Transcriptome profile in bursa of Fabricius reveals potential mode for stress-influenced immune function in chicken stress model.

BMC Genomics 2018 Dec 13;19(1):918. Epub 2018 Dec 13.

College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450002, China.

Background: The molecular mechanisms underlying stress-influenced immune function of chicken (Gallus Gallus) are not clear. The stress models can be established effectively by feeding chickens corticosterone (CORT) hormone. The bursa of Fabricius is a unique central immune organ of birds. Read More

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December 2018
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[Effectiveness of one-year insulin glargine and insulin glulisine basal-bolus treatment in people with type 2 diabetes. An analysis from drug reimbursement perspective].

Orv Hetil 2018 Dec;159(50):2122-2128

Medico-Pharmecon Kft. Budapest, Madarász V. u. 13., 1. ép. 5. em. 85., 1131.

Introduction: Being entitled for no patient co-payment, the Hungarian reimbursement condition of analogue insulins as part of basal-bolus treatment in type 2 diabetes mellitus (T2DM) requires that two HbA levels should achieve <8.0% target value within 12 months (measured two months apart) after switching from treatment with human insulins. Achieving this target, the treatment should be considered effective from drug reimbursement perspective. Read More

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December 2018

The Dietary Intervention of Transgenic Low-Gliadin Wheat Bread in Patients with Non-Celiac Gluten Sensitivity (NCGS) Showed No Differences with Gluten Free Diet (GFD) but Provides Better Gut Microbiota Profile.

Nutrients 2018 Dec 12;10(12). Epub 2018 Dec 12.

Departamento de Mejora Genética Vegetal, Instituto de Agricultura Sostenible (IAS-CSIC), 14004 Córdoba, Spain.

The study evaluated the symptoms, acceptance, and digestibility of bread made from transgenic low-gliadin wheat, in comparison with gluten free bread, in Non-coeliac gluten sensitivity (NCGS) patients, considering clinical/sensory parameters and gut microbiota composition. This study was performed in two phases of seven days each, comprising a basal phase with gluten free bread and an E82 phase with low-gliadin bread. Gastrointestinal clinical symptoms were evaluated using the Gastrointestinal Symptom Rating Scale (GSRS) questionnaire, and stool samples were collected for gluten immunogenic peptides (GIP) determination and the extraction of gut microbial DNA. Read More

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December 2018

Upregulation of Long Non-Coding RNA DRAIC Correlates with Adverse Features of Breast Cancer.

Noncoding RNA 2018 Dec 11;4(4). Epub 2018 Dec 11.

Department of Genetics and Cell Biology, College of Life Sciences, Nankai University, Tianjin 300071, China.

(also known as and ), is a long non-coding RNA associated with several types of cancer including prostate cancer, lung cancer, and breast cancer. Its expression is elevated in tumor tissues compared to adjacent benign tissues in breast cancer patients and is regulated by estrogen treatment in breast cancer cells. In addition, expression analysis of in more than 100 cell lines showed that expression is high in luminal and basal subtypes compared to claudin low subtype, suggesting a prognostic value of expression in breast cancer. Read More

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December 2018

Overexpression of Arabidopsis in Hexaploid Wheat ( L.) Alters Plant Development and Freezing Tolerance.

Int J Mol Sci 2018 Dec 11;19(12). Epub 2018 Dec 11.

Institute of Basic Biological Problems RAS, Pushchino 142290, Russia.

Jasmonates are plant hormones that are involved in the regulation of different aspects of plant life, wherein their functions and molecular mechanisms of action in wheat are still poorly studied. With the aim of gaining more insights into the role of jasmonic acid (JA) in wheat growth, development, and responses to environmental stresses, we have generated transgenic bread wheat plants overexpressing (), one of the key genes of the JA biosynthesis pathway. Analysis of transgenic plants showed that overexpression affects wheat development, including germination, growth, flowering time, senescence, and alters tolerance to environmental stresses. Read More

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December 2018

Simultaneous Quantitation of Isoprenoid Pyrophosphates in Plasma and Cancer Cells Using LC-MS/MS.

Molecules 2018 Dec 11;23(12). Epub 2018 Dec 11.

Department of Pharmacy Practice, University of Nebraska Medical Center, Omaha, NE 68198, USA.

Isoprenoids (IsoP) are an important class of molecules involved in many different cellular processes including cholesterol synthesis. We have developed a sensitive and specific LC-MS/MS method for the quantitation of three key IsoPs in bio-matrices, geranyl pyrophosphate (GPP), farnesyl pyrophosphate (FPP), and geranylgeranyl pyrophosphate (GGPP). LC-MS/MS analysis was performed using a Nexera UPLC System connected to a LCMS-8060 (Shimadzu Scientific Instruments, Columbia, MD) with a dual ion source. Read More

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December 2018

Generation of A Mucor circinelloides Reporter Strain-A Promising New Tool to Study Antifungal Drug Efficacy and Mucormycosis.

Genes (Basel) 2018 Dec 7;9(12). Epub 2018 Dec 7.

Departamento de Genética y Microbiología, Facultad de Biología, Universidad de Murcia, 30100 Murcia, Spain.

Invasive fungal infections caused by Mucorales (mucormycosis) have increased worldwide. These life-threatening infections affect mainly, but not exclusively, immunocompromised patients, and are characterized by rapid progression, severe tissue damage and an unacceptably high rate of mortality. Still, little is known about this disease and its successful therapy. Read More

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December 2018

Blast-induced traumatic brain injury: the experience from a level I trauma center in southern Thailand.

Authors:

Neurosurg Focus 2018 Dec;45(6):E7

OBJECTIVEIn the ongoing conflict in southern Thailand, the improvised explosive device (IED) has been a common cause of blast-induced traumatic brain injury (bTBI). The authors investigated the particular characteristics of bTBI and the factors associated with its clinical outcome.METHODSA retrospective cohort study was conducted on all patients who had sustained bTBI between 2009 and 2017. Read More

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December 2018

Unexpected diversity in eukaryotic transcription revealed by the retrotransposon hotspot family of Trypanosoma brucei.

Nucleic Acids Res 2018 Dec 14. Epub 2018 Dec 14.

Institute of Cell Biology, University of Bern, Bern, Switzerland.

The path from DNA to RNA to protein in eukaryotes is guided by a series of factors linking transcription, mRNA export and translation. Many of these are conserved from yeast to humans. Trypanosomatids, which diverged early in the eukaryotic lineage, exhibit unusual features such as polycistronic transcription and trans-splicing of all messenger RNAs. Read More

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December 2018

Molecular mechanisms in skeletal muscle underlying insulin resistance in lean women with polycystic ovary syndrome.

J Clin Endocrinol Metab 2018 Dec 13. Epub 2018 Dec 13.

Molecular Physiology Section, Department of Nutrition, Exercise and Sports, University of Copenhagen, Denmark.

Context: Skeletal muscle molecular mechanisms underlying insulin resistance in women with polycystic ovary syndrome (PCOS) are poorly understood.

Objective: To provide insight into mechanisms regulating skeletal muscle insulin resistance in lean women with PCOS.

Participants And Methods: A hyperinsulinemic-euglycemic clamp with skeletal muscle biopsies was performed. Read More

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December 2018

Maternal high fat diet programs hypothalamic-pituitary-adrenal function in adult rat offspring.

Psychoneuroendocrinology 2018 Dec 6;102:128-138. Epub 2018 Dec 6.

Department of Neurology, First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China. Electronic address:

Maternal environmental factors such as diet have profound effects on offspring development and later health. The hypothalamic-pituitary-adrenal (HPA) axis is an important stress neuroendocrine system that is subject to programming by early life challenges. The present study was further to investigate whether maternal high fat diet (HFD) exposure during rat pregnancy and lactation can alter the HPA axis activity in adult male offspring. Read More

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December 2018
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Arsenic trioxide and/or copper sulfate induced apoptosis and autophagy associated with oxidative stress and perturbation of mitochondrial dynamics in the thymus of Gallus gallus.

Chemosphere 2018 Dec 4;219:227-235. Epub 2018 Dec 4.

College of Wildlife Resources, Northeast Forestry University, Harbin 150040, Heilongjiang Province, China. Electronic address:

Arsenic (As) and copper (Cu) are ubiquitous environmental contaminants that are hazardous to the immune system. Our objective was to investigate the toxicity and potential mechanisms of thymus exposure to As and/or Cu. A chicken model was established by adding arsenic trioxide (AsO; 30 mg/kg), copper sulfate (CuSO; 300 mg/kg), and simultaneously both elements in the basal diet. Read More

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December 2018