158 results match your criteria Bacteriophage[Journal]


My scientific life.

Authors:
Margarita Salas

Bacteriophage 2016 15;6(4):e1271250. Epub 2016 Dec 15.

Centro de Biología Molecular "Severo Ochoa" (CSIC-UAM), Universidad Autónoma , Canto Blanco , Madrid, Spain.

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http://dx.doi.org/10.1080/21597081.2016.1271250DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5221747PMC
December 2016
2 Reads

Erratum.

Authors:

Bacteriophage 2016 4;6(4):e1271201. Epub 2017 Jan 4.

[This corrects the article DOI: 10.1080/21597081.2015. Read More

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http://dx.doi.org/10.1080/21597081.2016.1271201DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5221743PMC
January 2017

Félix Hubert d'Herelle (1873-1949): History of a scientific mind.

Bacteriophage 2016 4;6(4):e1270090. Epub 2017 Jan 4.

Program in History of Science and Medicine, Yale University , New Haven, CT, USA.

The discovery of bacteriophage one century ago by the French-Canadian Félix d'Herelle set off controversies as to the nature of bacteriophage as well as over the priority and credit for this discovery. The background and life of d'Herelle reveals a complex, self-taught outsider in science who was strongly influenced by his admiration of Louis Pasteur, but also his attachment to the philosophical positions of early 17th century philosophers, especially Francis Bacon. D'Herelle left substantial unpublished writings on his philosophical musings toward the end of his life. Read More

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http://dx.doi.org/10.1080/21597081.2016.1270090DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5221746PMC
January 2017
1 Read

Testing a proposed paradigm shift in analysis of phage DNA packaging.

Bacteriophage 2016 4;6(4):e1268664. Epub 2017 Jan 4.

Department of Biochemistry, The University of Texas Health Science Center , San Antonio, TX, USA.

We argue that a paradigm shift is needed in the analysis of phage DNA packaging. We then test a prediction of the following paradigm shift-engendering hypothesis. The motor of phage DNA packaging has two cycles: (1) the well-known packaging ATPase-driven (type 1) cycle and (2) a proposed back-up, shell expansion/contraction-driven (type 2) cycle that reverses type 1 cycle stalls by expelling accidentally packaged non-DNA molecules. Read More

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http://dx.doi.org/10.1080/21597081.2016.1268664DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5221748PMC
January 2017
2 Reads

Structural proteins of bacteriophage Ef11.

Bacteriophage 2016 4;6(4):e1251381. Epub 2016 Nov 4.

Department of Genomic Medicine, J Craig Venter Institute , Rockville, MD, USA.

ϕEf11, a temperate bacteriophage, was isolated by induction from a root canal isolate of . Sequence analysis suggested that the ϕEf11 genome included a contiguous 8 gene module whose function was related to head structure assembly and another module of 10 contiguous genes whose products were responsible for tail structure assembly. SDS-PAGE analysis of virions of a ϕEf11 derivative revealed 11 well-resolved protein bands. Read More

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http://dx.doi.org/10.1080/21597081.2016.1251381DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5221750PMC
November 2016
7 Reads

Fecal microbiota transplantation to fight infections and other intestinal diseases.

Bacteriophage 2016 21;6(4):e1251380. Epub 2016 Oct 21.

Max Planck Institute for Molecular Genetics, Berlin, Germany; Institute for Medical Microbiology, Unversity of Zürich, Zürich, Switzerland; Max Planck Institute of Colloids and Interfaces, Potsdam, Germany; Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.

We have analyzed fecal bacterial and viral communities of a patient with recurrent infection (rCDI) who was cured by fecal microbiota transplantation (FMT). The "Zürich Patient" experienced immediate cure and has remained free of symptoms for now over 5 y. Donor-similar bacterial compositions after 4. Read More

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https://www.tandfonline.com/doi/full/10.1080/21597081.2016.1
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http://dx.doi.org/10.1080/21597081.2016.1251380DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5221744PMC
October 2016
4 Reads

Phagebiotics in treatment and prophylaxis of healthcare-associated infections.

Bacteriophage 2016 21;6(4):e1251379. Epub 2016 Oct 21.

G. N. Gabrichevsky Research Institute for Epidemiology and Microbiology , Moscow, Russia.

We have developed a phagebiotic composition using 8 virulent bacteriophages (2 strains of each species) which are able to lyse , , and . The unique character of the developed composition is ensured by particular properties of each bacteriophage comprising the preparation, including their range of lytic activity toward specific bacterial pathogens, morphology of their plaques, cycle of their development, restriction profile of their DNAs, specificity of their genomes (based on complete genome sequencing), and other properties. The preparation did not produce any signs of acute or chronic intoxication in the experimental animals. Read More

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http://dx.doi.org/10.1080/21597081.2016.1251379DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5221749PMC
October 2016
5 Reads

Comparative genomics of 9 novel bacteriophages.

Bacteriophage 2016 Jul-Sep;6(3):e1220349. Epub 2016 Aug 5.

School of Life Sciences, University of Nevada Las Vegas , Las Vegas, NV, USA.

American Foulbrood Disease, caused by the bacterium , is one of the most destructive diseases of the honeybee, . Our group recently published the sequences of 9 new phages with the ability to infect and lyse . Here, we characterize the genomes of these phages, compare them to each other and to other sequenced phages, and putatively identify protein function. Read More

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http://dx.doi.org/10.1080/21597081.2016.1220349DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5056774PMC
August 2016
10 Reads

Phage therapy dosing: The problem(s) with multiplicity of infection (MOI).

Authors:
Stephen T Abedon

Bacteriophage 2016 Jul-Sep;6(3):e1220348. Epub 2016 Aug 11.

Department of Microbiology, The Ohio State University , Mansfield, OH, USA.

The concept of bacteriophage multiplicity of infection (MOI) - ratios of phages to bacteria - historically has been less easily applied than many phage workers would prefer or, perhaps, may be aware. Here, toward clarification of the concept, I discuss multiplicity of infection in terms of semantics, history, mathematics, pharmacology, and actual practice. For phage therapy and other biocontrol purposes it is desirable, especially, not to employ MOI to describe what phage quantities have been applied during dosing. Read More

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http://dx.doi.org/10.1080/21597081.2016.1220348DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5056779PMC
August 2016
3 Reads

Bacteriophages safely reduce contamination in pet food and raw pet food ingredients.

Bacteriophage 2016 Jul-Sep;6(3):e1220347. Epub 2016 Aug 5.

Intralytix, Inc. , Baltimore, MD, USA.

Contamination of pet food with is a serious public health concern, and several disease outbreaks have recently occurred due to human exposure to tainted pet food. The problem is especially challenging for raw pet foods (which include raw meats, seafood, fruits, and vegetables). These foods are becoming increasingly popular because of their nutritional qualities, but they are also more difficult to maintain -free because they lack heat-treatment. Read More

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http://dx.doi.org/10.1080/21597081.2016.1220347DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5056775PMC
August 2016
3 Reads

Testing hypotheses for the presence of tRNA genes in mycobacteriophage genomes.

Bacteriophage 2016 Jul-Sep;6(3):e1219441. Epub 2016 Aug 5.

Department of Biology, Bucknell University , Lewisburg, PA, USA.

The presence of tRNA genes in bacteriophages has been explained on the basis of codon usage (tRNA genes are retained in the phage genome if they correspond to codons more common in the phage than in its host) or amino acid usage (independent of codon, the amino acid corresponding to the retained tRNA gene is more common in the phage genome than in the bacterial host). The existence of a large database of sequenced mycobacteriophages, isolated on the common host , allows us to test the above hypotheses as well as explore other hypotheses for the presence of tRNA genes. Our analyses suggest that amino acid rather than codon usage better explains the presence of tRNA genes in mycobacteriophages. Read More

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http://dx.doi.org/10.1080/21597081.2016.1219441DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5056768PMC
August 2016
3 Reads

Characterization of novel lytic phage and defining their combinatorial virulence using the OmniLog® system.

Bacteriophage 2016 Jul-Sep;6(3):e1219440. Epub 2016 Aug 5.

Biological Defense Research Directorate, Naval Medical Research Center-Frederick , Fort Detrick, MD USA.

Skin and soft tissue infections (SSTI) caused by methicillin resistant (MRSA) are difficult to treat. Bacteriophage (phage) represent a potential alternate treatment for antibiotic resistant bacterial infections. In this study, 7 novel phage with broad lytic activity for were isolated and identified. Read More

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http://dx.doi.org/10.1080/21597081.2016.1219440DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5056778PMC
August 2016
4 Reads

Transmission of phage by glassy-winged sharpshooters, a vector of .

Bacteriophage 2016 Jul-Sep;6(3):e1218411. Epub 2016 Aug 2.

Department of Plant Pathology and Microbiology, Texas A&M University, College Station, TX, USA; Center for Phage Technology, Texas A&M University, College Station, TX, USA.

subsp. () is the causal agent of Pierce's Disease (PD) of grapevines and is vectored by the glassy-winged sharpshooter (GWSS, ). Previously we have reported the development of a bacteriophage (phage) based biocontrol system for PD, but no information on insect transmission of phages has been reported. Read More

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http://dx.doi.org/10.1080/21597081.2016.1218411DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5056766PMC
August 2016
23 Reads

The impact of orally administered phages on host immune response and surrounding microbial communities.

Bacteriophage 2016 Jul-Sep;6(3):e1211066. Epub 2016 Jul 13.

Department of Animal Sciences, Purdue University , West Lafayette, IN, USA.

Numerous studies have shown the efficacy of phage therapy in reducing foodborne pathogen carriage in food animals. Fewer studies have focused on host reactions, especially in terms of phage-mediated acute immune responses and effects on the gut microbiome. Here we administered O157:H7 phages in low (single dose of 10 PFU) or high (single dose of 10 PFU) quantities to mice. Read More

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https://www.tandfonline.com/doi/full/10.1080/21597081.2016.1
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http://dx.doi.org/10.1080/21597081.2016.1211066DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5056770PMC
July 2016
7 Reads

The diverse genetic switch of enterobacterial and marine telomere phages.

Bacteriophage 2016 Apr-Jun;6(2):e1148805. Epub 2016 Feb 18.

Federal Institute for Risk Assessment, Department of Biological Safety , Berlin, Germany.

Temperate bacteriophages possess a genetic switch which regulates the lytic and lysogenic cycle. The genomes of the enterobacterial telomere phages N15, PY54 and ϕKO2 harbor a primary immunity region (immB) comprising genes for the prophage repressor, the lytic repressor and a putative antiterminator, similar to CI, Cro and Q of lambda, respectively. Moreover, N15 and ϕKO2 contain 3 related operator (OR) sites between cI and cro, while only one site (OR3) has been detected in PY54. Read More

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http://dx.doi.org/10.1080/21597081.2016.1148805DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4951001PMC
September 2016
16 Reads

Grete Kellenberger-Gujer: Molecular biology research pioneer.

Bacteriophage 2016 Apr-Jun;6(2):e1148805. Epub 2016 Apr 5.

Department of Medicine, Division of Infectious Disease, University of California , San Diego, La Jolla, CA.

Grete Kellenberger-Gujer was a Swiss molecular biologist who pioneered fundamental studies of bacteriophage in the mid-20(th) century at the University of Geneva. Her life and career stories are reviewed here, focusing on her fundamental contributions to our early understanding of phage biology via her insightful analyses of phenomena such as the lysogenic state of a temperate phage (λ), genetic recombination, radiation's in vivo consequences, and DNA restriction-modification; on her creative personality and interactions with peers; and how her academic advancement was affected by gender, societal conditions and cultural attitudes of the time. Her story is important scientifically, putting into perspective features of the scientific community from just before the molecular biology era started through its early years, and also sociologically, in illustrating the numerous "glass ceilings" that, especially then, often hampered the advancement of creative women. Read More

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http://dx.doi.org/10.1080/21597081.2016.1173168DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4951002PMC
September 2016
1 Read

CTXϕ: Exploring new alternatives in host factor-mediated filamentous phage replications.

Bacteriophage 2016 Apr-Jun;6(2):e1128512. Epub 2016 Feb 11.

Institute for Integrative Biology of the Cell (I2BC), Université Paris-Saclay, CEA, CNRS, Univ. Paris Sud , Gif sur Yvette, France.

For a long time Ff phages from Escherichia coli provided the majority of the knowledge about the rolling circle replication mechanism of filamentous phages. Host factors involved in coliphages replication have been fully identified. Based on these studies, the function of Rep protein as the accessory helicase directly implicated in filamentous phage replication was considered a paradigm. Read More

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http://dx.doi.org/10.1080/21597081.2015.1128512DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4951000PMC
September 2016
1 Read

Bacteriophage MS2 genomic RNA encodes an assembly instruction manual for its capsid.

Bacteriophage 2016 Jan-Mar;6(1):e1157666. Epub 2016 Mar 2.

Department of Biology and Mathematics & York Center for Complex Systems Analysis, University of York , York, UK.

Using RNA-coat protein crosslinking we have shown that the principal RNA recognition surface on the interior of infectious MS2 virions overlaps with the known peptides that bind the high affinity translational operator, TR, within the phage genome. The data also reveal the sequences of genomic fragments in contact with the coat protein shell. These show remarkable overlap with previous predictions based on the hypothesis that virion assembly is mediated by multiple sequences-specific contacts at RNA sites termed Packaging Signals (PSs). Read More

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http://dx.doi.org/10.1080/21597081.2016.1157666DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4836477PMC
March 2016
11 Reads
7 Citations

Bacteriophage P2.

Bacteriophage 2016 Jan-Mar;6(1):e1145782. Epub 2016 Feb 18.

Department of Molecular and Cell Biology, University of California , Berkeley, CA, USA.

P2 is the original member of a highly successful family of temperate phages that are frequently found in the genomes of gram-negative bacteria. This article focuses on the organization of the P2 genome and reviews current knowledge about the function of each open reading frame. Read More

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http://dx.doi.org/10.1080/21597081.2016.1145782DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4836473PMC
February 2016
1 Read

T7 ejectosome assembly: A story unfolds.

Bacteriophage 2016 Jan-Mar;6(1):e1128513. Epub 2016 Feb 18.

Institute of Microbiology and Molecular Biology, University of Hohenheim , Stuttgart, Germany.

T7 phage DNA is transported from the capsid into the host cytoplasm across the cell wall by an ejectosome comprised of the viral proteins gp14, gp15 and gp16. Prior to infection, these proteins form the so-called internal core in the mature virion. Gp16 was shown to associate with pure phospholipid bilayers while gp15 bound to DNA. Read More

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http://dx.doi.org/10.1080/21597081.2015.1128513DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4836469PMC
February 2016
2 Reads

phage TP901-1 as a model for virion assembly.

Bacteriophage 2016 Jan-Mar;6(1):e1123795. Epub 2016 Jan 6.

School of Microbiology, University College Cork, Cork, Ireland; APC Microbiome Institute, University College Cork, Cork, Ireland.

Phages infecting pose a serious threat to the dairy fermentation sector. Consequently, they are among the most thoroughly characterized Gram positive-infecting phages. The majority of lactococcal phages belong to the tailed family of phages named the . Read More

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http://dx.doi.org/10.1080/21597081.2015.1123795DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4836478PMC
January 2016
9 Reads

Experimental bacteriophage treatment of honeybees () infected with , the causative agent of American Foulbrood Disease.

Bacteriophage 2016 Jan-Mar;6(1):e1122698. Epub 2016 Jan 5.

School of Life Sciences, University of Nevada , Las Vegas, Nevada, USA.

American Foulbrood Disease (AFB) is an infection of honeybees caused by the bacterium . One potential remedy involves using biocontrol, such as bacteriophages (phages) to lyse . Therefore, bacteriophages specific for were isolated to determine their efficacy in lysing cells. Read More

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http://www.tandfonline.com/doi/full/10.1080/21597081.2015.11
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http://dx.doi.org/10.1080/21597081.2015.1122698DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4836486PMC
January 2016
4 Reads

Counteracting selection for antibiotic-resistant bacteria.

Bacteriophage 2016 Jan-Mar;6(1):e1096996. Epub 2016 Mar 7.

Department of Clinical Microbiology and Immunology, Sackler School of Medicine, Tel Aviv University , Tel Aviv , Israel.

The occurrence of antibiotic-resistant bacterial pathogens is on the rise because antibiotics exert selection pressure that kills only the antibiotic-sensitive pathogens. Sanitation and cleansing of hospital surfaces and the skin of medical personnel do not counteract this selective pressure, but rather indiscriminately reduce total pathogens on treated surfaces. Here, we discuss two recently introduced genetic strategies, based on temperate bacteriophages as DNA-delivery vehicles, that aim to sensitize bacteria to antibiotics and selectively kill the antibiotic-resistant ones. Read More

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http://dx.doi.org/10.1080/21597081.2015.1096996DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4836461PMC
March 2016
13 Reads

Development of expanded host range phage active on biofilms of multi-drug resistant .

Bacteriophage 2016 Jan-Mar;6(1):e1096995. Epub 2016 Jan 5.

Department of Molecular Virology and Microbiology, Baylor College of Medicine , Houston, TX, USA.

Phage therapy is a promising treatment of multi-drug resistant (MDR) bacterial infections but is limited by the narrow host range of phage. To overcome this limitation, we developed a host range expansion (HRE) protocol that expands the host range of -specific phage by cycles of co-incubation of phage with multiple strains. Application of the HRE protocol to a mixture of 4 phages, using 16 strains for development, resulted in undefined phage mixtures with greatly expanded host range. Read More

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http://www.tandfonline.com/doi/full/10.1080/21597081.2015.10
Publisher Site
http://dx.doi.org/10.1080/21597081.2015.1096995DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4836484PMC
January 2016
6 Reads

Bacteriophage administration significantly reduces colonization and shedding by -challenged mice without deleterious side effects and distortions in the gut microbiota.

Bacteriophage 2015 Oct-Dec;5(4):e1088124. Epub 2015 Aug 28.

Intralytix, Inc. ; Baltimore, MD USA.

We used a mouse model to establish safety and efficacy of a bacteriophage cocktail, ShigActive™, in reducing fecal counts after oral challenge with a susceptible strain. Groups of inbred C57BL/6J mice challenged with strain S43-NalAcR were treated with a phage cocktail (ShigActive™) composed of 5 lytic bacteriophages and ampicillin. The treatments were administered (i) 1 h after, (ii) 3 h after, (iii) 1 h before and after, and (iv) 1 h before bacterial challenge. Read More

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http://dx.doi.org/10.1080/21597081.2015.1088124DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4745833PMC
August 2015
3 Reads

Pre-early functions of bacteriophage T5 and its relatives.

Authors:
John Davison

Bacteriophage 2015 Oct-Dec;5(4):e1086500. Epub 2015 Aug 25.

INRA de Versailles (retired) ; Versailles, France.

Coliphage T5 injects its DNA in 2 steps: the first step transfer (FST) region 7.9% is injected and its genes are expressed and only then does the remainder (second step transfer, SST) of its DNA enter the cell. In the FST region, only 2 essential genes ( and ) have been identified and a third () non-essential gene codes for a deoxyribonucleotide 5' monophosphatase. Read More

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http://dx.doi.org/10.1080/21597081.2015.1086500DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4743489PMC
August 2015
1 Read
5 Citations

Adding pieces to the puzzle: New insights into bacteriophage diversity from integrated research-education programs.

Bacteriophage 2015 Oct-Dec;5(4):e1084073. Epub 2015 Aug 18.

Department of Biological Sciences; University of Pittsburgh ; Pittsburgh, PA USA.

Bacteriophages are the dark matter of the biological universe: the population is vast and replete with novel genes whose function is unknown. The genomic insights such as the mosaic architecture gleaned from perhaps 2,000 currently sequenced bacteriophage genomes is far from representative of the total number phage particles in the biosphere - about 10ˆ31. The recent comparative analysis of 627 mycobacteriophages isolated on Mycobacterium smegmatis mc2 155 is the most extensive examination yet in pursuit of this question. Read More

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http://dx.doi.org/10.1080/21597081.2015.1084073DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4743490PMC
August 2015
7 Reads

Isolation and characterization of a novel phage lysin active against , a honeybee pathogen.

Bacteriophage 2015 Oct-Dec;5(4):e1080787. Epub 2015 Aug 12.

School of Life Sciences; University of Nevada ; Las Vegas, NV USA.

is the causative agent of American foulbrood (AFB) disease which affects early larval stages during honeybee development. Due to its virulence, transmissibility, capacity to develop antibiotic resistance, and the inherent resilience of its endospores, is extremely difficult to eradicate from infected hives which often must be burned. AFB contributes to the worldwide decline of honeybee populations, which are crucial for pollination and the food supply. Read More

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http://dx.doi.org/10.1080/21597081.2015.1080787DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4743491PMC
August 2015
3 Reads

Protection of bacteriophage Y2 from UV-induced damage by natural compounds.

Bacteriophage 2015 Oct-Dec;5(4):e1074330. Epub 2015 Jul 24.

Institute of Food and Beverage Innovation; Zurich University of Applied Sciences; Wädenswil, Switzerland; Institute of Food; Nutrition and Health; ETH Zurich; Zürich, Switzerland.

Bacteriophages have regained much attention as biocontrol agents against bacterial pathogens. However, with respect to stability, phages are biomolecules and are therefore sensitive to a number of environmental influences. UV-irradiation can readily inactivate phage infectivity, which impedes their potential application in the plant phyllosphere. Read More

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http://dx.doi.org/10.1080/21597081.2015.1074330DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4743488PMC
July 2015
1 Read

How WWII and the old Turkish mass standard led a Greek to a scientific career.

Bacteriophage 2016 Jan-Mar;6(1):e1093065. Epub 2015 Oct 27.

Department of Biochemistry, University of Utah , Salt Lake City, UT, USA.

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http://dx.doi.org/10.1080/21597081.2015.1093065DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5153855PMC
October 2015

Experimental evolution and bacterial resistance: (co)evolutionary costs and trade-offs as opportunities in phage therapy research.

Bacteriophage 2015 Apr-Jun;5(2):e1050153. Epub 2015 May 21.

Institute of Integrative Biology ; ETH Zürich; Zürich, Switzerland.

Antagonistic coevolution between bacteria and phages (reciprocal selection for resistance and infectivity) has been demonstrated in a wide range of natural ecosystems, as well as experimental populations of microbes, yet exploiting knowledge of coevolution for the prophylactic and therapeutic use of phages is under-explored. In this addendum to our recent paper we discuss how real-time coevolution studies using experimental populations of bacteria and phages can provide novel insight into the changes in bacterial phenotypes that result from resistance evolution against coevolving phages, and how this may ultimately improve our understanding of phage therapy and ability to design effective treatments. Read More

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http://dx.doi.org/10.1080/21597081.2015.1050153DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4588173PMC
May 2015
2 Reads

An insight into staphylococcal pathogenicity island-mediated interference with phage late gene transcription.

Bacteriophage 2015 Apr-Jun;5(2):e1028608. Epub 2015 Jun 11.

Departments of Microbiology and Medicine; New York University School of Medicine and Program in Molecular Pathogenesis; Skirball Institute ; New York, NY USA.

Staphylococcal pathogenicity islands (SaPIs) are ∼15 kb chromosomally located mobile elements that parasitize "helper" phages which provide a de-repressor protein plus virion and lysis proteins which enable the release of infectious SaPI particles in very high titers. All SaPIs interfere with the reproduction of their helper phages, using 3 different mechanisms. The logic of SaPI reproduction requires that these interference mechanisms do not totally block phage production, as this would be lethal for them as well as for the phage. Read More

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http://dx.doi.org/10.1080/21597081.2015.1028608DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4588161PMC

How long can bacteriophage λ change its mind?

Bacteriophage 2015 Jan-Mar;5(1):e1012930. Epub 2015 Jan 30.

Department of Biology; University of Copenhagen ; Copenhagen, Denmark.

A key event in the lifecycle of a temperate bacteriophage is the choice between lysis and lysogeny upon infection of a susceptible host cell. In a recent paper, we showed that a prolonged period exists after the decision to lysogenize, during which bacteriophage λ can abandon the initial decision, and instead develop lytically, as a response to the accumulation of the late lytic regulatory protein Q. Here, we present evidence that expression of Q does not induce replication of λ DNA, suggesting that the DNA to be packaged into the resulting phage progeny was already present at the time of the initial decision to lysogenize. Read More

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http://dx.doi.org/10.1080/21597081.2015.1012930DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4422806PMC
January 2015
8 Reads

A small-scale experiment of using phage-based probiotic dietary supplement for prevention of E. coli traveler's diarrhea.

Bacteriophage 2015 Jul-Sep;5(3):e1074329. Epub 2015 Jul 24.

Gabrichevsky Moscow Research Institute for Epidemiology and Microbiology ; Moscow, Russia.

Traveler's diarrhea (TD) is caused by Escherichia coli in 30% of cases. We have developed a phage cocktail for prophylaxis of TD caused by E.coli, Shigella flexneri, Shigella sonnei, Salmonella enterica, Listeria monocytogenes or Staphylococcus aureus, and investigated its effectiveness against infection caused by the non-pathogenic Lac (-) strain of E. Read More

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http://dx.doi.org/10.1080/21597081.2015.1074329DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4589986PMC
July 2015
4 Reads

Characterization of the initial steps in the T7 DNA ejection process.

Bacteriophage 2015 Jul-Sep;5(3):e1056904. Epub 2015 Jun 2.

Structure of Macromolecules Department; Centro Nacional de Biotecnología; Consejo Superior de Investigaciones Científicas ; Cantoblanco , Madrid, Spain ; Instituto Madrileño de Estudios Avanzados en Nanociencia (IMDEA Nanociencia) ; Cantoblanco , Madrid, Spain.

A specialized complex, the tail, is the most common strategy employed by bacterial viruses to deliver their genome without disrupting cell integrity. T7 has a short, non-contractile tail formed by a tubular structure surrounded by fibers. Recent studies showed that incubation of the virus with lipopolysaccharides (LPS) resulted in complete delivery of the viral genome, demonstrating for the first time that LPS are the T7 receptor. Read More

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http://dx.doi.org/10.1080/21597081.2015.1056904DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4589982PMC
June 2015
2 Reads

Antimicrobial bacteriophage-derived proteins and therapeutic applications.

Bacteriophage 2015 Jul-Sep;5(3):e1062590. Epub 2015 Jun 23.

Animal Biosciences and Biotechnology Laboratory; NEA; Agricultural Research Service; US Department of Agriculture ; Beltsville, MD USA.

Antibiotics have the remarkable power to control bacterial infections. Unfortunately, widespread use, whether regarded as prudent or not, has favored the emergence and persistence of antibiotic resistant strains of human pathogenic bacteria, resulting in a global health threat. Bacteriophages (phages) are parasites that invade the cells of virtually all known bacteria. Read More

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http://www.tandfonline.com/doi/full/10.1080/21597081.2015.10
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http://dx.doi.org/10.1080/21597081.2015.1062590DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4590002PMC
June 2015
29 Reads

Phage on the stage.

Bacteriophage 2015 Jul-Sep;5(3):e1062589. Epub 2015 Jun 22.

Department of Integrated Science & Technology; James Madison University ; Harrisonburg, VA USA ; Department of Biological Sciences; University of Mary Washington ; Fredericksburg, VA USA.

The resurgence of interest in bacteriophages for use in combating antibiotic resistant bacteria is coincident with an urgent call for more effective science education practices, including hands-on learning opportunities. To address this issue, a number of solutions have been proposed, including a large educational experiment, begun in 2007 by the Howard Hughes Medical Institute and currently involving over 85 colleges and universities, which has students discovering unique phages, obtaining images, and purifying phage DNA. A subset of these phage genomes is sequenced and analyzed using bioinformatics tools. Read More

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http://dx.doi.org/10.1080/21597081.2015.1062589DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4588534PMC
June 2015
4 Reads

Bioinformatics as a first-line approach for understanding bacteriophage transcription.

Bacteriophage 2015 Jul-Sep;5(3):e1062588. Epub 2015 Jun 24.

Institute of Physiology and Biochemistry; Faculty of Biology; University of Belgrade ; Belgrade, Serbia.

Current approach to understanding bacteriophage transcription strategies during infection includes a combination of experimental and bioinformatics approaches, which is often time and resource consuming. Given the exponentially growing number of sequenced bacteriophage genomes, it becomes sensible asking to what extent one can understand bacteriophage transcription by using bioinformatics methods alone. We here argue that a suitable choice of computational methods may provide a highly efficient first-line approach for underst-anding bacteriophage transcription. Read More

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http://dx.doi.org/10.1080/21597081.2015.1062588DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4588165PMC
June 2015
2 Reads

Serendipity and the times.

Bacteriophage 2015 Jul-Sep;5(3):e1059003. Epub 2015 Jun 9.

Distinguished Professor Emeritus of Biology; American Cancer Society Research Professor; University of Oregon ; Eugene, OR USA.

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http://dx.doi.org/10.1080/21597081.2015.1059003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4588157PMC

Life in science.

Authors:
Fred Eiserling

Bacteriophage 2015 Jul-Sep;5(3):e1050154. Epub 2015 Jun 25.

UCLA retired , Los Angeles, CA USA.

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http://dx.doi.org/10.1080/21597081.2015.1050154DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4588223PMC

Giuseppe Bertani (1923-2015).

Bacteriophage 2015 Apr-Jun;5(2):e1054060. Epub 2015 May 27.

Department of Molecular and Cell Biology; University of California ; Berkeley, CA USA.

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http://dx.doi.org/10.1080/21597081.2015.1054060DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4588218PMC

My life with Mu.

Authors:
Ariane Toussaint

Bacteriophage 2015 Apr-Jun;5(2):e1034336. Epub 2015 Apr 28.

Université Libre de Bruxelles; Génétique et physiologie bactérienne (LGPB) ; Campus de Gosselies - CP300 ; Charleroi (Gosselies), Belgium.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4588224PMC

Life In Science.

Bacteriophage 2015 Jan-Mar;5(1):e997143. Epub 2015 Jan 26.

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http://dx.doi.org/10.1080/21597081.2014.997143DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4422799PMC
January 2015

Phage therapy of pulmonary infections.

Authors:
Stephen T Abedon

Bacteriophage 2015 Jan-Mar;5(1):e1020260. Epub 2015 Apr 18.

Department of Microbiology; The Ohio State University ; Mansfield, OH USA.

It is generally agreed that a bacteriophage-associated phenomenon was first unambiguously observed one-hundred years ago with the findings of Twort in 1915. This was independently followed by complementary observations by d'Hérelle in 1917. D'Hérelle's appreciation of the bacteriophage phenomenon appears to have directly led to the development of phages as antibacterial agents within a variety of contexts, including medical and agricultural. Read More

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http://dx.doi.org/10.1080/21597081.2015.1020260DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4422798PMC
April 2015
7 Reads

The lambda - P22 problem.

Authors:
Hans-W Ackermann

Bacteriophage 2015 Jan-Mar;5(1):e1017084. Epub 2015 Apr 11.

Department of Microbiology-Infectiology and Immunology; Medical School; Laval University ; Quebec, QC Canada.

Lambda and P22 are members of 2 families of tailed phages and have limited genomic relationships. Both form hybrids with many phages. P22 appears as a hybrid of mixed ancestry. Read More

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http://dx.doi.org/10.1080/21597081.2015.1017084DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4422791PMC
April 2015
1 Read

Bacteriophage HK022 Nun protein arrests transcription by blocking lateral mobility of RNA polymerase during transcription elongation.

Bacteriophage 2014 30;4:e32187. Epub 2014 Jul 30.

Department of Microbiology and Immunology, Columbia University, New York, NY USA.

Coliphage HK022 excludes phage λ by subverting the λ antitermination system and arresting transcription on the λ chromosome. The 12 kDa HK022 Nun protein binds to λ nascent transcript through its N-terminal Arginine Rich Motif (ARM), blocking access by λ N and arresting transcription via a C-terminal interaction with RNA polymerase. In a purified in vitro system, we recently demonstrated that Nun arrests transcription by restricting lateral movement of transcription elongation complex (TEC) along the DNA register, thereby freezing the translocation state. Read More

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http://dx.doi.org/10.4161/bact.32187DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4124055PMC

Bacteriophage behavioral ecology: How phages alter their bacterial host's habits.

Bacteriophage 2014 8;4:e29866. Epub 2014 Jul 8.

Department of Infection, Immunity and Inflammation; University of Leicester; Leicester, UK.

Bacteriophages have an essential gene kit that enables their invasion, replication, and production. In addition to this "core" genome, they can carry "accessory" genes that dramatically impact bacterial biology, and presumably boost their own success. The content of phage genomes continue to surprise us by revealing new ways that viruses impact bacterial biology. Read More

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http://dx.doi.org/10.4161/bact.29866DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4124054PMC
July 2014
11 Reads
8 Citations

Molecular basis of RNA polymerase promoter specificity switch revealed through studies of bacteriophage transcription regulator.

Bacteriophage 2014 29;4:e29399. Epub 2014 May 29.

RIKEN Systems and Structural Biology Center; Tsurumi-ku, Yokohama Japan ; RIKEN Structural Biology Laboratory; Tsurumi-ku, Yokohama Japan.

Transcription initiation is the central point of gene expression regulation. Understanding of molecular mechanism of transcription regulation requires, ultimately, the structural understanding of consequences of transcription factors binding to DNA-dependent RNA polymerase (RNAP), the enzyme of transcription. We recently determined a structure of a complex between transcription factor gp39 encoded by a bacteriophage and RNAP holoenzyme. Read More

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http://dx.doi.org/10.4161/bact.29399DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4124052PMC
May 2014
26 Reads

Morphotypes of virus-like particles in two hydrothermal vent fields on the East Scotia Ridge, Antarctica.

Bacteriophage 2014 2;4:e28732. Epub 2014 Apr 2.

British Antarctic Survey; Cambridge, UK.

Viruses from extreme environments are still largely unexplored and may harbor unseen genetic potential. Here, we present a first glance at the morphological diversity of virus like particles (VLPs) from an environment that is extreme in more than one respect: two recently discovered hydrothermal vent fields on the East Scotia Ridge in the Southern Ocean near Antarctica. They are the southernmost hydrothermal sites found to date and have been shown to present a new biogeographic province, containing several new macrofaunal species and associated microbial organisms. Read More

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http://dx.doi.org/10.4161/bact.28732DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4124060PMC
April 2014
2 Reads
1 Citation

Flap endonuclease of bacteriophage T7: Possible roles in RNA primer removal, recombination and host DNA breakdown.

Bacteriophage 2014 11;4:e28507. Epub 2014 Mar 11.

The Department of Biological Chemistry and Molecular Pharmacology; Harvard Medical School; Boston, MA USA.

Gene 6 protein of bacteriophage T7 has 5'-3'-exonuclease activity specific for duplex DNA. We have found that gene 6 protein also has flap endonuclease activity. The flap endonuclease activity is considerably weaker than the exonuclease activity. Read More

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http://dx.doi.org/10.4161/bact.28507DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4124056PMC
March 2014
13 Reads