4,720 results match your criteria Bacterial Vaginosis


Comparison between a novel molecular tool and conventional methods for diagnostic classification of bacterial vaginosis: is integration of the two approaches necessary for a better evaluation?

New Microbiol 2020 Jun 13;43(3). Epub 2020 Jun 13.

Laboratory Medicine, San Bassiano Hospital, Bassano del Grappa, Italy.

The etiological cause of bacterial vaginosis (BV) is the change of the vaginal ecosystem characterized by a decrease of lactobacilli and an increase of other germs, such as Gardnerella vaginalis and Atopobium vaginae. Molecular tools have revolutionized the diagnosis of these conditions. The aim of this paper was to compare results obtained from 158 vaginal swabs collected from women aged between 18 and 59 years old and subjected to microscopic evaluation (Nugent Score), culture and to the multiparametric molecular assay Vaginitis and Vaginosis Multiplex-Tandem (MT) PCR (AU27117) - Nuclear Laser Medicine. Read More

View Article

Download full-text PDF

Source

Lateral Gene Transfer Shapes Diversity of spp.

Front Cell Infect Microbiol 2020 23;10:293. Epub 2020 Jun 23.

Department of Medical Microbiology and Immunology, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI, United States.

spp. are pathognomonic for bacterial vaginosis, which increases the risk of preterm birth and the transmission of sexually transmitted infections. spp. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3389/fcimb.2020.00293DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7324480PMC

Testing the regulatory framework in South Africa - a single-blind randomized pilot trial of commercial probiotic supplementation to standard therapy in women with bacterial vaginosis.

BMC Infect Dis 2020 Jul 10;20(1):491. Epub 2020 Jul 10.

Division of Medical Virology, Faculty of Health Sciences, Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Anzio Road, Observatory, Cape Town, 7925, South Africa.

Background: Bacterial vaginosis (BV) increases HIV risk and adverse reproductive outcomes. Standard-of-care (SOC) for BV are antibiotics; however, cure rates are low. Probiotics for vaginal health may be useful in improving cure and recurrence although the regulatory framework governing probiotics and the conduct of randomized clinical trials to evaluate these has not been established in South Africa. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12879-020-05210-4DOI Listing

Vaginal Microbiota Evaluation and Lactobacilli Quantification by qPCR in Pregnant and Non-pregnant Women: A Pilot Study.

Front Cell Infect Microbiol 2020 19;10:303. Epub 2020 Jun 19.

Laboratorio de Bacteriología, Instituto de Microbiología, Colegio de Ciencias Biológicas y Ambientales, Universidad San Francisco de Quito, Quito, Ecuador.

Pregnancy outcomes and women's health are directly affected by vaginal microbiota. This microbiota consists of a dynamic ecosystem of various microbes in different ratios, which in healthy conditions protect the vaginal epithelium from infections. However, cases of vaginal infection are regularly diagnosed in women of reproductive age, contributing to more severe outcomes. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3389/fcimb.2020.00303DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7318849PMC

Relationship between the Oral and Vaginal Microbiota of South African Adolescents with High Prevalence of Bacterial Vaginosis.

Microorganisms 2020 Jul 4;8(7). Epub 2020 Jul 4.

Department of Pathology, Institute of Infectious Disease and Molecular Medicine, University of Cape Town, 7925 Cape Town, South Africa.

Bacterial vaginosis (BV) and periodontal disease (PD) are characterised as bacterial dysbioses. Both are associated with an increased risk of poor pregnancy outcomes, yet it is unknown whether PD and BV are related. We characterised the oral microbiota of young South African females with a high prevalence of BV and investigated the association between oral communities and vaginal microbiota. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3390/microorganisms8071004DOI Listing

The apparition macrophage and Döderlein bacillus is negatively correlated in class I Papanicolaou smear: A morphological examination.

Diagn Cytopathol 2020 Jul 6. Epub 2020 Jul 6.

Department of Clinical Engineering and Medical Technology, Niigata University Health and Welfare, Niigata, Japan.

Background: Nonspecific vaginitis, also known as Bacterial vaginosis, unlike genital candidiasis and trichomoniasis, is caused by microbiome breakdown. Döderlein's bacillus are gram-positive bacillus that form a microbiome, reproduce in the female vagina after gaining sexual maturity, secrete lactic acid, and prevent the growth of other vaginitis-causing bacteria. Clue cell are squamous epithelial cells with Gardnerella sp. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1002/dc.24538DOI Listing

gen. nov., sp. nov., a novel propionic acid bacterium derived from human vaginal discharge.

Int J Syst Evol Microbiol 2020 Jul 6. Epub 2020 Jul 6.

Aix-Marseille Univ, IRD, AP-HM, SSA, VITROME, Marseille, France.

A Gram-stain-positive anaerobic rod-shaped bacterium, designated strain Marseille-P3275, was isolated using culturomics from the vaginal discharge of healthy French woman. Marseille-P3275 was non-motile and did not form spores. Cells had neither catalase nor oxidase activity. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1099/ijsem.0.004106DOI Listing

Association between Common Vaginal Infections and Cervical Non-HPV16/18 Infection in HPV-Vaccinated Women.

J Infect Dis 2020 Jul 2. Epub 2020 Jul 2.

Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, U.S.A.

Background: How vaginal infections like bacterial vaginosis (BV), Candida spp., and Trichomonas vaginalis (TV) affect persistence of human papillomavirus (HPV) infection is not well established. Our study aimed to evaluate the association between common vaginal infections and cervical non-HPV16/18 infection, as risk factors associated with persistence of non-vaccine HPV types will become increasingly relevant in the setting of HPV vaccination. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1093/infdis/jiaa384DOI Listing

Genital Micro-Organisms in Pregnancy.

Front Public Health 2020 16;8:225. Epub 2020 Jun 16.

Reproductive Medicine, Nova IVF Fertility, Ahmedabad, India.

The microbiome of the female genital tract may undergo changes in pregnancy due to metabolic, endocrinological, and immunological alterations. These dysbiotic states may cause infections which may ascend upwards to the feto-placental unit or may be seeded hematogenously. These low grade and often low virulent infectious states lead to chronic inflammatory states and maybe associated with adverse maternal and neonatal outcome. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3389/fpubh.2020.00225DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7308476PMC

Antimicrobial Susceptibility Testing of Metronidazole and Clindamycin against in Planktonic and Biofilm Formation.

Can J Infect Dis Med Microbiol 2020 16;2020:1361825. Epub 2020 Jun 16.

Department of Gynecology, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100026, China.

Background: Bacterial vaginosis (BV), one of the most common vaginal ecosystem-related microbiologic syndromes, is the most common disorder in women of reproductive age. () is the predominant species causing this infection. Our aim was to compare the antimicrobial susceptibilities of metronidazole and clindamycin against at planktonic and biofilm levels. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1155/2020/1361825DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7315270PMC

Analysis of HPV genotypes and liquid-based cervical cytology: Results from a tertiary academic center in northwestern Turkey.

Jpn J Infect Dis 2020 Jun 30. Epub 2020 Jun 30.

Duzce University, Faculty of Medicine, Department of Pathology&Cytology, Konuralp Campus, Turkey.

High-risk human papilloma virus (hrHPV) plays an important role in the cause of cervical cancer. The aim of this study is to investigate the distribution of HPV genotypes in the region and to correlate with the liquid-based-cytology (LBC) and colposcopic biopsy results. Furthermore, the potential relationship between HPV infections and bacterial vaginosis (BV) was investigated. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.7883/yoken.JJID.2020.072DOI Listing
June 2020
1.204 Impact Factor

Risk-based screening to identify reproductive tract infection among HIV-infected women desiring use of intrauterine contraceptives.

BMJ Sex Reprod Health 2020 Jul 1. Epub 2020 Jul 1.

Division of Epidemiology and Biostatistics, University of Cape Town, Cape Town, South Africa.

Background: Reproductive tract infections (RTIs) are a major cause of morbidity and mortality, yet RTI testing remains limited in resource-constrained settings. We assessed performance of an existing RTI risk assessment screening tool among women living with HIV (WLHIV) considering intrauterine contraceptive (IUC) use.

Methods: We conducted a cross-sectional analysis among WLHIV screened for participation in an IUC trial in Cape Town, South Africa (NCT01721798). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1136/bmjsrh-2019-200494DOI Listing

Is Gram-Positive? A Case Study of .

Microorganisms 2020 Jun 29;8(7). Epub 2020 Jun 29.

Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Korea.

is the most prevalent bacterial species in the human vaginal microbiome, and there have been few reports of its Gram-negative stain appearances despite the fact that the genus is universally described as Gram-positive. Here, using transmission electron microscopy, we reveal that the thinness of the cell wall (17.39 ± 0. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3390/microorganisms8070969DOI Listing

"It's just an issue and you deal with it… you just deal with it, you move on and you do it together.": Men's experiences of bacterial vaginosis and the acceptability of male partner treatment.

PLoS One 2020 29;15(6):e0235286. Epub 2020 Jun 29.

Melbourne Sexual Health Centre, Alfred Health, Melbourne, Victoria, Australia.

Bacterial vaginosis (BV) is a common vaginal infection among women of reproductive age. Increasing evidence suggests BV may be sexually transmitted indicating a potential role for the treatment of sexual partners. If partner treatment reduces BV recurrence in women, real-world success will depend on sexual partners' willingness to accept it. Read More

View Article

Download full-text PDF

Source
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0235286PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7323956PMC

Septic shock caused by and .

IDCases 2020 18;21:e00876. Epub 2020 Jun 18.

Service d'Anesthésie et Réanimation, CH de Saintonge, 11, Boulevard Ambroise Paré, 17100, Saintes, France.

Although bacterial vaginosis is the most common and benign vaginal infection worldwide, some cases of severe acute infections have been described in the literature. We report the case of a 57-year-old French female who developed a life-threatening postoperative peritonitis after a total hysterectomy with adnexectomy in the context of the removal of leiomyosarcoma. The microbiological analysis of the peritoneal fluid identified and The final diagnosis was a septic shock induced by an early onset peritonitis caused by and . Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.idcr.2020.e00876DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7306488PMC

Use of a Novel Couples' Verification Tool in a Male Partner Treatment Study of Women with Recurrent Bacterial Vaginosis.

Sex Transm Dis 2020 Jun 23. Epub 2020 Jun 23.

Division of Infectious Diseases, University of Alabama at Birmingham.

Verification of relationship status beyond self-report is an important aspect in sexually transmitted infection research, including partner treatment studies where primary sexual partners are targeted for enrollment. This exploratory study describes the use of a novel couples' verification tool in a male partner treatment study of women with bacterial vaginosis. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1097/OLQ.0000000000001225DOI Listing

Characterization of aerobic vaginitis in late pregnancy in a Chinese population: A STROBE-compliant study.

Medicine (Baltimore) 2020 Jun;99(25):e20732

Department of Laboratory Medicine, West China Second University Hospital.

This study aimed to analyze the clinical characteristics, responsible pathogens, and antibiotic sensitivity of aerobic vaginitis (AV) infection in women in late pregnancy in western China.We enrolled 246 pregnancy with AV (≥35 weeks gestation) and 204 reproductive non-pregnancy with AV from West China between January 2019 and December 2019. Then, bacterial culture, identification and antibiotic sensitivity testing were performed. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1097/MD.0000000000020732DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7310957PMC

Sensitivity, specificity, inclusivity and exclusivity of the updated Aptima Combo 2 assay, which provides detection coverage of the new diagnostic-escape Chlamydia trachomatis variants.

BMC Infect Dis 2020 Jun 16;20(1):419. Epub 2020 Jun 16.

World Health Organization Collaborating Centre for Gonorrhoea and Other Sexually Transmitted Infections (STIs), National Reference Laboratory for STIs, Department of Laboratory Medicine, Clinical Microbiology, Faculty of Medicine and Health, Örebro University, SE-701 85, Örebro, Sweden.

Background: Four new variants of Chlamydia trachomatis (nvCTs), detected in several countries, cause false-negative or equivocal results using the Aptima Combo 2 assay (AC2; Hologic). We evaluated the clinical sensitivity and specificity, as well as the analytical inclusivity and exclusivity of the updated AC2 for the detection of CT and Neisseria gonorrhoeae (NG) on the automated Panther system (Hologic).

Methods: We examined 1004 clinical AC2 samples and 225 analytical samples spiked with phenotypically and/or genetically diverse NG and CT strains, and other potentially cross-reacting microbial species. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s12879-020-05148-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7298785PMC
June 2020
2.613 Impact Factor

Formulation and technology development of vaginal pessaries with probiotic activity.

Ceska Slov Farm 2020 ;69(2):90-99

Lactobacilli use in treatment and prevention of the vaginal microflora disorders, such as bacterial vaginosis and vulvovaginal candidiasis, is highly promising. The objective of this study was is to develop formulation and technology of the extemporal Lactobacillus casei (L. casei) ІМВ В-7280-containing medicinal product in the form of vaginal pessaries. Read More

View Article

Download full-text PDF

Source
January 2020

Recovery of Vaginal Microbiota After Standard Treatment for Bacterial Vaginosis Infection: An Observational Study.

Microorganisms 2020 Jun 9;8(6). Epub 2020 Jun 9.

Global Health and Nutrition Science, DuPont Nutrition & Biosciences, Sokeritehtaantie 20, FIN-02460 Kantvik, Finland.

Vaginal microbiota dysbiosis and bacterial vaginosis (BV) affect negatively women's health. Understanding vaginal microbiota fluctuations in BV during and after antibiotic treatment would facilitate accurate decision-making on the treatment regimen, avoid unnecessary antibiotic use, and potentially mitigate recurrence. We investigated vaginal microbiota composition of 30 women with BV before and after 5-day metronidazole treatment and compared the results with 30 healthy women. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3390/microorganisms8060875DOI Listing

A population-specific optimized GeneXpert pooling algorithm for and to reduce cost of molecular STI screening in resource-limited settings.

J Clin Microbiol 2020 Jun 10. Epub 2020 Jun 10.

Department of Epidemiology, Rollins School of Public Health, Emory University, Atlanta, GA 30322.

The sexually transmitted infections (STIs) chlamydia (CT) and gonorrhea (NG) are often asymptomatic in women and undetected by syndromic management, leading to complications such as pelvic inflammatory disease, infertility, and ectopic pregnancy. Molecular testing, such as the GeneXpert CT/NG assay, is highly sensitive, but cost restraints preclude implementation of these technologies in resource-limited settings. Pooled testing is one strategy to reduce the cost-per-sample, but the extent of savings depends on disease prevalence. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1128/JCM.00176-20DOI Listing

Members of Prevotella genus distinctively modulate innate immune and barrier functions in a human three-dimensional endometrial epithelial cell model.

J Infect Dis 2020 Jun 9. Epub 2020 Jun 9.

Department of Obstetrics and Gynecology, College of Medicine-Phoenix, University of Arizona, Phoenix, USA.

Background: Prevotella species are commonly isolated from the reproductive tract of women with obstetric/gynecologic health complications. However, contributions of this genus to changes in the local microenvironment are not well characterized. Our objective was to evaluate species-specific effects of Prevotella on the human endometrial epithelium. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1093/infdis/jiaa324DOI Listing

In vitro characteristics of intravaginal Lactobacilli; why is L. iners detected in abnormal vaginal microbial flora?

Arch Gynecol Obstet 2020 Jun 7. Epub 2020 Jun 7.

Department of Microbiology, University of Occupational and Environmental Health, Kitakyushu, Japan.

Purpose: Our purpose is to investigate the reasons why Lactobacillus iners is detected in abnormal vaginal microbial flora.

Methods: In this study, in vitro characteristics of four type strains (L. crispatus, L. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00404-020-05634-yDOI Listing

Elevated Risk of Bacterial Vaginosis among Users of the Copper Intrauterine Device: A Prospective Longitudinal Cohort Study.

Clin Infect Dis 2020 Jun 6. Epub 2020 Jun 6.

Department of Epidemiology, University of Washington, Seattle, WA, USA.

Background: Limited evidence suggests that the non-hormonal contraceptive copper intrauterine device (Cu-IUD) may increase bacterial vaginosis (BV) risk, possibly due to increased volume and duration of menses, a common side effect of Cu-IUD use. While increases in bleeding typically resolve within 6-12 months following initiation, evaluations of the association between Cu-IUD and BV have not included more than six months of follow-up.

Methods: This secondary analysis of an HIV-1 prevention trial included 2,585 African women ages 18-45 followed for up to 33 months. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1093/cid/ciaa703DOI Listing

Factors associated with preterm birth in women undergoing cervical cerclage.

Eur J Obstet Gynecol Reprod Biol 2020 Aug 16;251:141-145. Epub 2020 May 16.

Fetal Medicine Unit, Elizabeth Garrett Anderson Obstetric Wing, University College London Hospital NHS Foundation Trust, 235 Euston Road, London NW1 2BU, UK; Elizabeth Garrett Anderson Institute for Women's Health, University College London, 86-96 Chenies Mews, Bloomsbury, London WC1E 6AU, UK.

Objectives: Cervical cerclage is used to manage women at high risk of late miscarriage (LM) and spontaneous preterm birth (PTB) due to factors such as history of cervical insufficiency (CI), uterine anomaly, cervical surgery and ultrasound (US) diagnosed cervical shortening. Urinary tract infection (UTI) and subsequent pyelonephritis, and bacterial infection are associated with PTB, but their role in PTB after cervical cerclage is unknown. We examined the relationship between UTI and bacterial vaginosis (BV), fetal fibronectin (fFN) test and PTB in women undergoing elective- or US-indicated cervical cerclage. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.ejogrb.2020.05.027DOI Listing

High prevalence of severe vitamin D deficiency during the first trimester in pregnant women in Switzerland and its potential contributions to adverse outcomes in the pregnancy.

Swiss Med Wkly 2020 May 28;150:w20238. Epub 2020 May 28.

Department of Obstetrics and Gynaecology, University Hospital Bern, Inselspital, University of Bern, Switzerland.

Purpose: Vitamin D is primarily known for its role in bone health. However, it has a much more diverse role in the human metabolism. Specifically, deficiency of vitamin D has recently been studied for its possible role in adverse pregnancy outcomes such as preeclampsia, gestational diabetes and preterm birth. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.4414/smw.2020.20238DOI Listing

Draft Genome Sequence of Actinomyces neuii UMB1295, Isolated from the Female Urinary Tract.

Microbiol Resour Announc 2020 Jun 4;9(23). Epub 2020 Jun 4.

Department of Biology, Loyola University Chicago, Chicago, Illinois, USA

is an opportunistic pathogen. Within the urogenital tract, it has been associated with bacterial vaginosis and overactive bladder symptoms. Here, we investigate a draft genome sequence of UMB1295, which was isolated from a catheterized urine sample from a woman with a urinary tract infection. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1128/MRA.00402-20DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7272554PMC

Exploring potential of vaginal Lactobacillus isolates from South African women for enhancing treatment for bacterial vaginosis.

PLoS Pathog 2020 Jun 4;16(6):e1008559. Epub 2020 Jun 4.

Department of Pathology, Institute of Infectious Disease and Molecular Medicine (IDM), University of Cape Town, Cape Town, South Africa.

Antibiotics continue to be the standard-of-care for bacterial vaginosis (BV), although recurrence rates are high. Vaginal probiotics may improve durability of BV treatment, although few probiotics for vaginal health contain Lactobacillus spp. that commonly colonize the lower female genital tract. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1371/journal.ppat.1008559DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7271994PMC

The interplay between immune system and microbiota in gynecological diseases: a narrative review.

Eur Rev Med Pharmacol Sci 2020 May;24(10):5676-5690

Dipartimento di Scienze della Salute della Donna, del Bambino e di Sanità Pubblica, Policlinico Universitario A. Gemelli IRCCS, Rome, Italy.

Objective: The vaginal microbiome is a dynamic environment, depending on the results of a complex interplay between microbiota and the host. In physiological conditions, Lactobacillus species are the most represented, regulating glycogen metabolism in order to maintain normal pH. Vaginal flora has been divided into five subtypes. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.26355/eurrev_202005_21359DOI Listing

Production and characterization of a monoclonal antibody against the sialidase of Gardnerella vaginalis using a synthetic peptide in a MAP8 format.

Appl Microbiol Biotechnol 2020 Jul 27;104(14):6173-6183. Epub 2020 May 27.

Facultad de Ciencias Químico Biológicas, Universidad Autónoma de Guerrero, Chilpancingo, Guerrero, Mexico.

Bacterial vaginosis is one of the most frequent vaginal infections. Its main etiological agent is Gardnerella vaginalis, which produces several virulence factors involved in vaginal infection and colonization, in particular, sialidase (SLD), a potential clinical biomarker that participates in immune response modulation and mucus degradation. The main objective of this work was the production and evaluation of a monoclonal antibody against G. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1007/s00253-020-10691-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7253150PMC

bacteremia in an elderly healthy male.

IDCases 2020 11;21:e00807. Epub 2020 May 11.

Department of Infectious Disease, Jersey Shore University Medical Center, Neptune, NJ, 07753, USA.

is an anaerobic gram variable organism, which commonly causes bacterial vaginosis in women. It is uncommon for this organism to cause a urinary tract infection in males. We present a case of bacteremia in the setting of urolithiasis in an otherwise immunocompetent elderly male. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.idcr.2020.e00807DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7242859PMC

Impact of Symbiosis Between and on Vaginal Dysbiosis: A Mini Review.

Front Cell Infect Microbiol 2020 8;10:179. Epub 2020 May 8.

Department of Biomedical Sciences, University of Sassari, Sassari, Italy.

The protozoon is responsible for trichomoniasis, a common sexually transmitted infection associated with an increased risk of HIV infection and adverse pregnancy outcomes. The protozoon has the surprising ability to establish a symbiotic relationship with other microorganisms. In fact, most isolates intracellularly host the vaginal bacterium and can harbor up to four dsRNA viruses. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3389/fcimb.2020.00179DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7226223PMC

The impact of cervical cytobrush sampling on cervico-vaginal immune parameters and microbiota relevant to HIV susceptibility.

Sci Rep 2020 May 22;10(1):8514. Epub 2020 May 22.

Department of Medicine, University of Toronto, Toronto, Canada.

The immunology and microbiota of the female genital tract (FGT) are key determinants of HIV susceptibility. Cervical cytobrush sampling is a relatively non-invasive method permitting the longitudinal assessment of endocervical immune cells, but effects on FGT immunology are unknown. Blood, cervico-vaginal secretions and cervical cytobrushes were collected from sexually transmitted infection (STI)-free women at baseline and after either 6 hours or 48 hours. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1038/s41598-020-65544-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7244754PMC

[Ten questions about bacterial vaginosis].

Gynecol Obstet Fertil Senol 2020 May 11. Epub 2020 May 11.

Maternité des Diaconnesses, 12-18, rue du Sergent Bauchat, 75012 Paris, France.

The physiopathology of bacterial vaginosis (BV), the ultimate stage of vaginal dysbiosis, has benefited from recent advances in molecular biology, highlighting, among others, the important role of A. vaginae. Certain immunological specificities (variants of TLR4, elevation of IL-1β, for example) explain the variations in the prevalence of this infection, the poor clinical and cellular inflammatory response and the promoting influence of BV on the acquisition and progression of some sexually transmitted infections. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.gofs.2020.05.003DOI Listing

Vaginal probiotic adherence and acceptability in Rwandan women with high sexual risk participating in a pilot randomised controlled trial: a mixed-methods approach.

BMJ Open 2020 May 19;10(5):e031819. Epub 2020 May 19.

Department of Clinical Infection, Microbiology and Immunology, Institute of Infection and Global Health, University of Liverpool, Liverpool, Merseyside, UK

Objectives: To evaluate adherence and acceptability of intermittent vaginal probiotic or antibiotic use to prevent bacterial vaginosis (BV) recurrence.

Design: Repeated adherence and acceptability assessments using mixed methods within a pilot randomised controlled trial.

Setting: Research clinic in Kigali, Rwanda. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1136/bmjopen-2019-031819DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7247375PMC

Randomized Trial of Lactin-V to Prevent Recurrence of Bacterial Vaginosis.

N Engl J Med 2020 05;382(20):1906-1915

From the Departments of Obstetrics, Gynecology, and Reproductive Sciences (C.R.C., S.N., A.H.) and Laboratory Medicine (L.G., S. Miller), University of California, San Francisco, San Francisco, the Department of Family Medicine, University of California, San Diego, San Diego (S. Morris), and Osel, Mountain View (T.P.) - all in California; Emmes, Rockville, MD (M.R.W., J.P.); the Department of Medicine, Ruth M. Rothstein CORE Center and Stroger Hospital of Cook County Health, Rush University Medical Center, Chicago (A.L.F.); and the Division of Infectious Diseases, Department of Medicine, Washington University, St. Louis (H.R.).

Background: Bacterial vaginosis affects 15 to 50% of women of reproductive age, and recurrence is common after treatment with an antibiotic agent. The high incidence of recurrence suggests the need for new treatments to prevent recurrent bacterial vaginosis.

Methods: We conducted a randomized, double-blind, placebo-controlled, phase 2b trial to evaluate the ability of CTV-05 (Lactin-V) to prevent the recurrence of bacterial vaginosis. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1056/NEJMoa1915254DOI Listing

Electrospun metronidazole-loaded nanofibers for vaginal drug delivery.

Drug Dev Ind Pharm 2020 Jun 25;46(6):1015-1025. Epub 2020 May 25.

Department of Pharmaceutical Technology, Faculty of Pharmacy, Gazi University, Ankara, Turkey.

To develop and characterize innovative vaginal dosage forms for the treatment of bacterial vaginosis (BV). This study is the first comparative evaluation of the metronidazole (MET)-loaded polyvinylpyrrolidone (PVP) nanofiber formulations on BV treatment. Vaginal nanofibers are one of the potential innovative dosage forms for BV treatment because of their flexible, mucoadhesive, and easy application in vaginal application which can be applied by the mucosal route. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1080/03639045.2020.1767125DOI Listing

as a Cause of Bacterial Vaginosis: Appraisal of the Evidence From Models.

Front Cell Infect Microbiol 2020 24;10:168. Epub 2020 Apr 24.

Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO, United States.

Koch's postulates dictate the use of experimental models to illustrate features of human disease and provide evidence for a singular organism as the cause. The underlying cause(s) of bacterial vaginosis (BV) has been debated in the literature for over half a century. In 1955, it was first reported that a bacterium now known as may be the cause of a condition (BV) resulting in higher vaginal pH, thin discharge, a fishy odor, and the presence of epithelial cells covered in bacteria. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3389/fcimb.2020.00168DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7193744PMC

Extra-vaginal Bacterial Colonization and Risk for Incident Bacterial Vaginosis in a Population of Women who Have Sex with Men.

J Infect Dis 2020 May 7. Epub 2020 May 7.

Fred Hutchinson Cancer Research Center, Seattle, WA.

Bacterial vaginosis (BV) is a common cause of vaginal discharge and associated with vaginal acquisition of BV-associated bacteria (BVAB). We used quantitative PCR assays to determine if presence or concentrations of BVAB in the mouth, anus, vagina, or labia prior to BV predict risk of incident BV in 72 women who have sex with men. Baseline vaginal and extra-vaginal colonization with Gardnerella spp. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1093/infdis/jiaa233DOI Listing

Five Percent Monolaurin Vaginal Gel for the Treatment of Bacterial Vaginosis: A Randomized Placebo-Controlled Trial.

J Low Genit Tract Dis 2020 Jul;24(3):277-283

Department of Internal Medicine, University of Iowa Carver College of Medicine, Iowa City, IA.

Objective: The aim of the study was to test the hypothesis that 5% monolaurin vaginal gel, a naturally occurring monoglyceride shown to have antimicrobial effects on vaginal pathogens without affecting Lactobacillus species, cures bacterial vaginosis (BV).

Materials And Methods: This was a multicenter, double-blinded, randomized controlled trial comparing 5% monolaurin vaginal gel to vehicle placebo (glycol-based) gel administered twice daily for 3 days. Nonpregnant, nonbreastfeeding women between ages 18 and 50 years were recruited and BV confirmed. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1097/LGT.0000000000000543DOI Listing

-Based Probiotics as Novel Antimicrobial Agents to Prevent and Treat Vaginal Infections.

Front Microbiol 2020 21;11:718. Epub 2020 Apr 21.

Department of Medicine, Medical Microbiology Section, University of Perugia, Perugia, Italy.

Vaginal infections affect 70% of women during their lifetimes and account for millions of annual doctors' visits. These infections are predominantly represented by vulvovaginal candidiasis (VVC) and bacterial vaginosis (BV). Although standard antimicrobial agents remain the major strategy for the prevention and treatment of vaginal infections, both VVC and BV are difficult to treat due to high rates of resistance and recurrence, high probability of complications, and negative effects on the vaginal microbiota. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3389/fmicb.2020.00718DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7186379PMC

The neovaginal microbiome of transgender women post-gender reassignment surgery.

Microbiome 2020 05 5;8(1):61. Epub 2020 May 5.

University of California, Los Angeles, CA, USA.

Background: Gender reassignment surgery is a procedure some transgender women (TW) undergo for gender-affirming purposes. This often includes the construction of a neovagina using existing penile and scrotal tissue and/or a sigmoid colon graft. There are limited data regarding the composition and function of the neovaginal microbiome representing a major gap in knowledge in neovaginal health. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1186/s40168-020-00804-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7201977PMC

Thoughts about .

Sex Transm Infect 2020 May 4. Epub 2020 May 4.

Infectious Diseases, Imperial College London, London, UK

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1136/sextrans-2020-054479DOI Listing

A phase 2, double-blind, multicenter, randomized, placebo-controlled, dose‑ranging study of the efficacy and safety of Astodrimer Gel for the treatment of bacterial vaginosis.

PLoS One 2020 4;15(5):e0232394. Epub 2020 May 4.

Royal Hallamshire and Sheffield Teaching Hospitals, Sheffield, United Kingdom.

Background: Astodrimer Gel contains a novel dendrimer intended to treat and prevent bacterial vaginosis. We assessed the efficacy and safety of Astodrimer Gel for treatment of bacterial vaginosis.

Methods: 132 women with bacterial vaginosis were randomized 1:1:1:1 to Astodrimer 0. Read More

View Article

Download full-text PDF

Source
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0232394PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7197797PMC

Is bacterial vaginosis associated with autoimmune antibody positivity?

Cytopathology 2020 May 2. Epub 2020 May 2.

Department of Gynecology and Obstetrics, Hacettepe University, Ankara, Turkey.

Objective: To evaluate the association between bacterial vaginosis (BV) and autoimmune antibody positivity.

Method: We evaluated Papanicolaou-stained cervicovaginal smears of 210 patients with poor obstetric history who were admitted to a special preconception counselling programme. Cytological specimens with various types of microorganisms except for BV, epithelial cell abnormalities and other non-neoplastic findings, including inflammation were excluded from the cohort in addition to patients with autoimmune and chronic inflammatory diseases. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1111/cyt.12846DOI Listing

Pathobionts in the Vaginal Microbiota: Individual Participant Data Meta-Analysis of Three Sequencing Studies.

Front Cell Infect Microbiol 2020 15;10:129. Epub 2020 Apr 15.

London School of Hygiene and Tropical Medicine, London, United Kingdom.

Sequencing studies have shown that optimal vaginal microbiota (VMB) are lactobacilli-dominated and that anaerobes associated with bacterial vaginosis (BV-anaerobes) are commonly present. However, they overlooked a less prevalent but more pathogenic group of vaginal bacteria: the pathobionts that cause maternal and neonatal infections and pelvic inflammatory disease. We conducted an individual participant data meta-analysis of three VMB sequencing studies that included diverse groups of women in Rwanda, South Africa, and the Netherlands (2,044 samples from 1,163 women in total). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3389/fcimb.2020.00129DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7174631PMC
April 2020
2.620 Impact Factor

Diagnosis and Treatment of Vaginal Discharge Syndromes in Community Practice Settings.

Clin Infect Dis 2020 Apr 30. Epub 2020 Apr 30.

Magee-Womens Research Institute, Pittsburgh, Pennsylvania, USA.

Background: Although vaginal symptoms are common, diagnosis of bacterial vaginosis (BV), vulvovaginal candidiasis (VVC), and Trichomonas vaginalis (TV) is not standardized. Diagnostic approaches and appropriateness of treatment were evaluated for women with symptoms of vaginitis who were seeking care at community practice sites.

Methods: Three hundred three symptomatic women, across 8 University of Pittsburgh Medical Center-affiliated clinics, were evaluated per standard office-based practice. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1093/cid/ciaa260DOI Listing

Conformal, waterproof electronic decals for wireless monitoring of sweat and vaginal pH at the point-of-care.

Biosens Bioelectron 2020 Jul 17;160:112206. Epub 2020 Apr 17.

School of Industrial Engineering, Purdue University, 315 N. Grant Street, West Lafayette, IN, 47907, USA; Weldon School of Biomedical Engineering, Purdue University, 206 S. Martin Jischke Drive, West Lafayette, IN, 47907, USA. Electronic address:

While the monitoring of pH has demonstrated to be an effective technique to monitor an individual's health state, the design of wearable biosensors is subject to critical challenges, such as high fabrication costs, thermal drift, sensitivity to moisture, and the limited applicability for users with metal allergies. This work describes the low-cost fabrication of waterproof electronic decals (WPEDs): highly conformable disposable biosensors capable of monitoring sweat and vaginal pH. WPEDs contain a polyaniline/silver microflakes sensing layer optimized for accurate impedance-based pH quantification across the clinically relevant range of variation of most biofluids. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.bios.2020.112206DOI Listing

A Randomized Controlled Open Label Crossover Trial to Study Vaginal Colonization of Orally Administered Lactobacillus Reuteri RC-14 and Rhamnosus GR-1 in Pregnant Women at High Risk for Preterm Labor.

Nutrients 2020 Apr 19;12(4). Epub 2020 Apr 19.

Department of Obstetrics & Gynecology, Emek Medical Center, Afula 1834111, Israel.

Lactobacilli administration has been suggested for the treatment and prevention of bacterial vaginosis, which increases the risk for preterm birth. We aimed to evaluate the vaginal colonization of lactobacilli orally administered to pregnant women at risk for preterm birth. We performed a randomized and controlled crossover study between January 2016 and May 2017. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3390/nu12041141DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7230520PMC

Incidence and Antimicrobial Susceptibilities of in Pregnant Females, Ehime University Hospital.

Rinsho Biseibutshu Jinsoku Shindan Kenkyukai Shi 2020 Apr;29(2):53-58

Department of Clinical Laboratory, Ehime University Hospital. Department of Infection Control Team, Ehime University Hospital.

Objective: usually colonizes the lower urogenital tract and has been occasionally associated with pelvic inflammatory disease, postpartum fever, preterm labor in pregnant females. The aim of this study was to investigate the incidence and antimicrobial susceptibilities of isolated from the urogenital tracts of pregnant females.

Methods: Specimens were obtained from the urogenital tract of pregnant females at Department of Obstetrics and Gynecology, Ehime University Hospital, between November 2014 and December 2017. Read More

View Article

Download full-text PDF

Source