1,104,425 results match your criteria Bacterial Vaginosis


Functionalized rifampicin-loaded nanostructured lipid carriers enhance macrophages uptake and antimycobacterial activity.

Colloids Surf B Biointerfaces 2018 Dec 4;175:306-313. Epub 2018 Dec 4.

Laboratório de Fitotecnologia, Escola de Farmácia, Universidade Federal de Ouro Preto, Ouro Preto, Brazil. Electronic address:

Tuberculosis is an infectious bacterial disease that causes millions of deaths worldwide. Current treatment recommended by WHO is effective, however it is an extensive and arduous process associated to severe adverse effects, which induces a low patient compliance and the emerging of multidrug resistant tuberculosis. Thus, as a main goal of this study, rifampicin nanoparticles were surface functionalized with a tuftsin-modifed peptide to selectively recognize receptors located on infected alveolar macrophages, enhancing nanoparticles uptake by these cells and improving antimycobacterial activity. Read More

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December 2018

Influence of the T to S mutation at the STMK motif on antibiotic resistance of penicillin binding protein 1A: A comprehensive computational study.

J Mol Graph Model 2018 Dec 7;87:185-191. Epub 2018 Dec 7.

Molecular Biology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran.

The emergence of antibiotic resistance has attracted the attention of scientists and scientific circles over the decades. β-Lactam antibiotics resistance is a worldwide therapeutic challenge in bacterial infections, mediated through several mechanisms of which mutations in Penicillin Binding Proteins (PBPs) are an important issue, making critical therapeutic problems in the human population. Accordingly, investigating the dynamic structures of mutant variants could result in a profound understanding of such a specific resistance. Read More

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December 2018

Housefly larvae (Musca domestica) significantly accelerates degradation of monensin by altering the structure and abundance of the associated bacterial community.

Ecotoxicol Environ Saf 2018 Dec 12;170:418-426. Epub 2018 Dec 12.

College of Veterinary Medicine, China Agricultural University, No. 2 Yuan Ming Yuan West Road, Hai Dian District, Beijing 100193, China. Electronic address:

Vermicomposting of livestock manure using housefly larvae is a promising biotechnology for waste reduction and control of antibiotic pollution. Monensin (MON), an ionophore polyether antibiotic (IPA), is widely used in broiler feed to control coccidiosis. However, MON residues in litter have become a major source of pollution in the environment. Read More

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December 2018
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Efficacy and safety of partial splenic embolization for hypersplenism in pre- and post-liver transplant patients: A 16-year comparative analysis.

Clin Imaging 2018 Nov 27;54:71-77. Epub 2018 Nov 27.

Department of Radiology, Division of Vascular and Interventional Radiology, Columbia University Medical Center, 177 Fort Washington Ave, Milstein Hospital - 4 Hudson North, New York, NY 10032, United States of America.

Purpose: To report the effect of partial splenic embolization (PSE) on hematological indices and the procedure's safety in pre- and post-liver transplant (LT) patients.

Materials And Methods: A single-center retrospective study evaluating all patients who underwent PSE over a 16-year period was performed. Inclusion criteria were splenomegaly confirmed by imaging and at least one of the following cytopenias: hemoglobin ≤10 g/dL, WBC count ≤1500 μL, or platelet count ≤100,000 μL. Read More

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November 2018
1 Read

Draft genome sequence of human-associated streptogramin resistant Staphylococcus aureus.

J Glob Antimicrob Resist 2018 Dec 12. Epub 2018 Dec 12.

Bacterial Epidemiology and Antimicrobial Resistance Research Unit, USDA-ARS, Athens, Georgia, USA. Electronic address:

Objectives: Staphylococcus aureus is one of the leading causes of nosocomial and community-acquired infections. Treatment of those infections with macrolide-lincosamide-streptogramin (MLS) antibiotics has led to resistance to those antibiotics through various mechanisms. S. Read More

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December 2018

Establishing a decision-support system for eco-design of biological wastewater treatment: A case study of bioaugmented constructed wetland.

Bioresour Technol 2018 Dec 7;274:425-429. Epub 2018 Dec 7.

Section of Sanitary Engineering, Department of Water Management, Delft University of Technology, Delft 2628CN, The Netherlands; Veolia Water Technologies Techno Center Netherlands B.V., Tanthofdreef 21, 2623 EW Delft, The Netherlands.

Deep treatment is a common approach to enhance pollutant removal for biological wastewater treatment technologies (BWTTs), and life cycle assessment (LCA) holds substantial advantages to support process optimization. However, there lacks of LCA-based benchmarks that cover human-nature nexuses and stakeholder involvement, which limits the guidance and eco-design of BWTTs. This study proposed a decision-support system (DSS) by linking LCA with Water Quality Model and Conjoint Analysis. Read More

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December 2018
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Key role of cyromazine in the distribution of antibiotic resistance genes and bacterial community variation in aerobic composting.

Bioresour Technol 2018 Dec 3;274:418-424. Epub 2018 Dec 3.

College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi 712100, China.

The risks that have not been noted so far have come from the use of non-antibiotics. In this study, non-antibiotic drug (cyromazine) was used in composting to investigate its possible effects on the distribution of ARGs and changes of bacterial community. Results showed that cyromazine increased the abundances of highly-risky ARGs (bla and bla), and heavy metal resistance genes (MRGs). Read More

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December 2018
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Improved SILAC method for double labeling of bacterial proteome.

J Proteomics 2018 Dec 12. Epub 2018 Dec 12.

Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, China. Electronic address:

Stable isotope labeling with amino acids in cell culture (SILAC) is a robust proteomics method with advantages such as reproducibility and easy handling. This method is popular for the analysis of mammalian cells. However, amino acid conversion in bacteria decreases the labeling efficiency and quantification accuracy, limiting the application of SILAC in bacterial proteomics to auxotrophic bacteria or to single labeling with lysine. Read More

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December 2018

Epidemiology and etiology of diarrhea in UK military personnel serving on the United Nations Mission in South Sudan in 2017: A prospective cohort study.

Travel Med Infect Dis 2018 Dec 12. Epub 2018 Dec 12.

Academic Department of Military Medicine, Medical Directorate, Defence Medical Services, Birmingham, UK.

Background: . Diarrhea is a well-established problem in travellers, with military personnel at especially high risk. This study aimed to characterise the spectrum of pathogens causing diarrhea in UK military personnel in South Sudan, and assess the utility of culture-independent testing for etiology and antimicrobial resistance in a logistically challenging and austere environment. Read More

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December 2018

Variety matters: Diverse functions of monocyte subtypes in vascular inflammation and atherogenesis.

Vascul Pharmacol 2018 Dec 12. Epub 2018 Dec 12.

Cardiology and Angiology, Philipps-University Marburg, Marburg, Germany. Electronic address:

Monocytes are important mediators of the innate immunity by recognizing and attacking especially bacterial pathogens but also play crucial roles in various inflammatory diseases, including vascular inflammation and atherosclerosis. Maturation, differentiation and function of monocytes have been intensively explored for a long time in innumerable experimental and clinical studies. Monocytes do not represent a uniform cell type but could be further subdivided into subpopulations with distinct features and functions. Read More

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December 2018

The role of the wzy gene in lipopolysaccharide biosynthesis and pathogenesis of avian pathogenic Escherichia coli.

Microb Pathog 2018 Dec 12. Epub 2018 Dec 12.

Shanghai Veterinary Research Institute, The Chinese Academy of Agricultural Sciences (CAAS), 518 Ziyue Road, Shanghai, 200241, PR China. Electronic address:

Avian pathogenic Escherichia coli (APEC) causes severe respiratory and systemic diseases in poultry. The wzy gene encodes the O-antigen polymerase (Wzy), which plays an important role in the synthesis of the lipopolysaccharide (LPS) of bacteria. However, the function of the wzy gene in APEC remains unclear. Read More

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December 2018

Evaluation of antioxidant and antimicrobial potential of a novel Himalayan plant Reinwardtia indica dumort: Scientifically unexplored.

Microb Pathog 2018 Dec 12. Epub 2018 Dec 12.

Department of Kriyasharir Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, 221005, India.

Reinwardtia indica (Lineceae) is a medicinal plant cultivated in the Himalayan region. It is effectively used in folk medicines for the treatment of various health complications. In the present study, the shade dried leaves and stem were extracted in three different solvents such as ethyl acetate, ethanol, and hydro-alcoholic. Read More

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December 2018

Chitosan-hydroxypropyl methylcellulose tioconazole films: A promising alternative dosage form for the treatment of vaginal candidiasis.

Int J Pharm 2018 Dec 12. Epub 2018 Dec 12.

Instituto de Química Rosario (IQUIR, CONICET-UNR), Suipacha 531, Rosario S2002LRK, Argentina; Área Técnica Farmacéutica, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 531, Rosario S2002LRK, Argentina. Electronic address:

Vaginal candidiasis is considered a frequent opportunistic mucosal infection and the second most common cause of vaginitis after bacterial vaginosis. In this work, different vaginal films based on chitosan, hydroxypropyl methylcellulose and blends of these polymers containing tioconazole, were developed and thoroughly characterized to improve the conventional therapeutics of vaginal candidiasis. Mechanical properties, swelling, adhesiveness, morphology, antifungal activity, hemocompatibility and cytotoxicity were evaluated. Read More

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December 2018

Epidemiology and Effects of Bacterial Infections in Patients With Cirrhosis Worldwide.

Gastroenterology 2018 Dec 12. Epub 2018 Dec 12.

Unit of Internal Medicine and Hepatology (UIMH), Department of Medicine - DIMED, University of Padova, Padova, Italy. Electronic address:

Background And Aims: Bacterial infections are common and life-threatening in patients with cirrhosis. Little is known about the epidemiology of bacterial infections in different regions. We performed a multicenter prospective intercontinental study to assess the prevalence and outcomes of bacterial and fungal infections in patients with cirrhosis. Read More

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December 2018
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Cardiopulmonary Bypass Time Predicts Early Postoperative Enterobacteriaceae Bloodstream Infection.

Ann Thorac Surg 2018 Dec 12. Epub 2018 Dec 12.

Division of Cardiovascular Surgery, Department of Surgery, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan; Division of Cardiovascular Surgery, Department of Surgery, E-DA hospital and College of Medicine, I-Shou University, Kaohsiung, Taiwan. Electronic address:

Background: A bloodstream infection in patients undergoing cardiovascular surgery is crucial because it can result in significantly worse outcomes. However, microbiological patterns have rarely been investigated in these patients.

Methods: We retrospectively reviewed the data of 1,041 adult patients undergoing cardiovascular surgeries using cardiopulmonary bypass from January 2013 to December 2017 at the National Cheng Kung University Hospital, Tainan, Taiwan. Read More

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December 2018

Phage-based assay for rapid detection of bacterial pathogens in blood by Raman spectroscopy.

J Immunol Methods 2018 Dec 12. Epub 2018 Dec 12.

Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Viale F. Stagno d'Alcontres 31, 98166 Messina, Italy. Electronic address:

Sepsis is a systemic inflammatory response ensuing from presence and persistence of microorganisms in the bloodstream. The possibility to identify them at low concentrations may improve the problem of human health and therapeutic outcomes. So, sensitive and rapid diagnostic systems are essential to evaluate bacterial infections during the time, also reducing the cost. Read More

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December 2018

Improving the quality and workflow of bacterial genome sequencing and analysis: paving the way for a Switzerland-wide molecular epidemiological surveillance platform.

Swiss Med Wkly 2018 Dec 15;148:w14693. Epub 2018 Dec 15.

SIB Swiss Institute of Bioinformatics, Lausanne, Switzerland.

Facing multidrug resistant (MDR) bacterial pathogens is one of the most important challenges for our society. The spread of highly virulent and resistant pathogens can be described using molecular typing technologies; in particular, whole genome sequencing (WGS) data can be used for molecular typing purposes with high resolution. WGS data analysis can explain the spatiotemporal patterns of pathogen transmission. Read More

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December 2018

The composition and structure of the intestinal microflora of Anguilla marmorata at different growth rates: a deep sequencing study.

J Appl Microbiol 2018 Dec 15. Epub 2018 Dec 15.

Jimei University, Xiamen, 361021, China.

Aims: The aim of this study was to determine the intestinal microflora of Anguilla marmorata at different growth rates and to identify potential probiotic/pernicious bacteria.

Methods And Results: Bacterial communities from 8 different fish intestinal sites (including the intestinal contents and the intestinal mucosa) from three fish groups (3 fast-, 2 medium-, and 3 stunted-growth samples), 2 water samples, and 1 diet sample were characterized by Illumina next-generation sequencing. The data revealed that the predominant genera (relative abundance of bacteria genera >1%) in the intestine of fast- and medium-growth groups were Cetobacterium, Edwardsiella, Clostridium, Lactococcus, Bacteroides, Plesiomonas, and Akkermansia. Read More

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December 2018

An important role of -fucose biosynthesis and protein fucosylation genes in Arabidopsis immunity.

New Phytol 2018 Dec 15. Epub 2018 Dec 15.

Department of Energy Plant Research Laboratory, East Lansing, MI, 48824, USA.

Plants mount coordinated immune responses to defend against pathogens. However, the cellular components required for plant immunity are not fully understood. The jasmonate-mimicking coronatine (COR) toxin produced by Pseudomonas syringae pv. Read More

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December 2018

Characterization of two steroid hydroxylases from different Streptomyces spp. and their ligand-bound and -unbound crystal structures.

FEBS J 2018 Dec 15. Epub 2018 Dec 15.

Department of Life Science and Biochemical Engineering, Sunmoon University, Asansi, 31460, Republic of Korea.

Bacterial cytochrome P450 (CYP) enzymes are involved in the hydroxylation of various endogenous substrates while using a heme molecule as a cofactor. CYPs have gained biotechnological interest as useful biocatalysts capable of altering chemical structures by adding a hydroxyl group in a regiospecific manner. Here, we identified, purified, and characterized two CYP154C4 proteins from Streptomyces sp. Read More

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December 2018

The structure of mammalian β-mannosidase provides insight into β-mannosidosis and Nystagmus.

FEBS J 2018 Dec 15. Epub 2018 Dec 15.

Department of Biochemistry and Groupe de Recherche Axé sur la Structure des Protéines, McGill University, Montreal, QC, H3G 0B1, Canada.

β-mannosidase is a lysosomal enzyme from the glycosyl hydrolase family 2 that cleaves the single β(1-4)-linked mannose at the non-reducing end of N-glycosylated proteins, and plays an important role in the polysaccharide degradation pathway. Mutations in the MANBA gene, which encodes the β-mannosidase, can lead to the lysosomal storage disease β-mannosidosis, as well as nystagmus, an eye condition characterized by involuntary eye movements. Here, we present the first structures of a mammalian β-mannosidase in both the apo and mannose-bound forms. Read More

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December 2018

H, C, and N backbone resonance assignments for KPC-2, a class A serine-β-lactamase.

Biomol NMR Assign 2018 Dec 14. Epub 2018 Dec 14.

Department of Chemistry and Biochemistry, Miami University, 651 E. High St, Oxford, OH, 45056, USA.

The ever-increasing occurrence of antibiotic resistance presents a major threat to public health. Specifically, resistance conferred by β-lactamases places the efficacy of currently available antibiotics at risk. Klebsiella pneumoniae carbapenemase-2 (KPC-2) is a β-lactamase that enables carbapenem resistance and represents a clear and present danger to global public health. Read More

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December 2018
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In Schizophrenia, Increased Plasma IgM/IgA Responses to Gut Commensal Bacteria Are Associated with Negative Symptoms, Neurocognitive Impairments, and the Deficit Phenotype.

Neurotox Res 2018 Dec 15. Epub 2018 Dec 15.

Department of Psychiatry, University of Toronto, Toronto, ON, Canada.

Increased gut permeability (leaky gut) with increased translocation of Gram-negative bacteria plays a role in the gut-brain axis through effects on systemic immune-inflammatory processes. Deficit schizophrenia is characterized by an immune-inflammatory response combined with a deficit in natural IgM antibodies to oxidative-specific epitopes (OSEs), which are a first-line defense against bacterial infections. This study measured plasma IgA/IgM responses to 5 Gram-negative bacteria in association with IgM responses to malondialdehyde (MDA) and azelaic acid in 80 schizophrenia patients (40 with the deficit syndrome and 40 without) and in 38 healthy controls. Read More

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December 2018

Effective mucosal live attenuated Salmonella vaccine by deleting phosphotransferase system component genes ptsI and crr.

J Microbiol 2018 Oct 2. Epub 2018 Oct 2.

Radiation Biotechnology Division, Korea Atomic Energy Research Institute, Jeongeup, 56212, Republic of Korea.

Salmonella enterica is a major human pathogen that causes invasive non-typhoidal Salmonellosis (iNTS), resulting in significant morbidity and mortality. Although a number of pre-clinical and clinical studies have reported on the feasibility of developing a safe and effective vaccine against iNTS, there have been no licensed Salmonella vaccines available to protect against NTS strains. Vaccine formulations of highest priority for NTS are live attenuated vaccines, which can elicit effective induction of intestinal mucosal and intracellular bacteria-specific cell mediated immune responses. Read More

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October 2018
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Antibiotic-resistant clones in Gram-negative pathogens: presence of global clones in Korea.

Authors:
Kwan Soo Ko

J Microbiol 2018 Oct 2. Epub 2018 Oct 2.

Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine, Suwon, 16419, Republic of Korea.

Antibiotic resistance is a global concern in public health. Antibiotic-resistant clones can spread nationally, internationally, and globally. This review considers representative antibiotic-resistant Gram-negative bacterial clones-CTX-M- 15-producing ST131 in Escherichia coli, extended-spectrum β-lactamase-producing ST11 and KPC-producing ST258 in Klebsiella pneumoniae, IMP-6-producing, carbapenem-resistant ST235 in Pseudomonas aeruginosa, and OXA-23-producing global clone 2 in Acinetobacter baumannii-that have disseminated worldwide, including in Korea. Read More

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October 2018

Specific features of immune complexes in patients with sarcoidosis and pulmonary tuberculosis.

Immunol Res 2018 Dec 14. Epub 2018 Dec 14.

FGBU "St. Petersburg Scientific Research Institute of Phthisiopulmonology" of the Ministry of Health of Russia, St. Petersburg, Russia.

Clinical and radiological features of tuberculosis and sarcoidosis are quite overlapping, and therefore, a diagnostic dilemma often persists. There are no commonly accepted criteria for the diagnosis of sarcoidosis due to the lack of data on the etiology of the disease. The exclusion of tuberculosis in every patient with suspected sarcoidosis is a mandatory stage of diagnosis, especially in countries with a high burden of tuberculosis. Read More

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December 2018

Floral and Foliar Source Affect the Bee Nest Microbial Community.

Microb Ecol 2018 Dec 14. Epub 2018 Dec 14.

Graduate Program in Microbiology, University of California, 900 University Ave., Riverside, CA, 92521, USA.

Managed pollinators such as the alfalfa leafcutting bee, Megachile rotundata, are essential to the production of a wide variety of agricultural crops. These pollinators encounter a diverse array of microbes when foraging for food and nest-building materials on various plants. To test the hypothesis that food and nest-building source affects the composition of the bee-nest microbiome, we exposed M. Read More

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December 2018

F-doped TiO microporous coating on titanium with enhanced antibacterial and osteogenic activities.

Sci Rep 2018 Dec 14;8(1):17858. Epub 2018 Dec 14.

State Key Laboratory for Mechanical Behavior of Materials, Xi'an Jiaotong University, Xi'an, 710049, China.

To enhance bacterial resistance and osteogenesis of titanium (Ti) -based implants, TiO/calcium-phosphate coatings (TiCP) doped with various amounts of fluorine (F) (designated as TiCP-F1, TiCP-F6, and TiCP-F9) were prepared on Ti by micro-arc oxidation. The F doped TiCP coatings possess a microporous structure (pore size of 3-4 μm in average diameter) which is evenly covered by nano-grains of 30-60 nm in size. Successful F incorporation into TiCP was determined by X-ray photoelectron spectroscopy, and it shows weak influence on the microstructure, phase compositions, surface roughness and wettability of TiCP. Read More

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December 2018

The novel EHEC gene asa overlaps the TEGT transporter gene in antisense and is regulated by NaCl and growth phase.

Sci Rep 2018 Dec 14;8(1):17875. Epub 2018 Dec 14.

Lehrstuhl für Mikrobielle Ökologie, Wissenschaftszentrum Weihenstephan, Technische Universität München, Weihenstephaner Berg 3, 85354, Freising, Germany.

Only a few overlapping gene pairs are known in the best-analyzed bacterial model organism Escherichia coli. Automatic annotation programs usually annotate only one out of six reading frames at a locus, allowing only small overlaps between protein-coding sequences. However, both RNAseq and RIBOseq show signals corresponding to non-trivially overlapping reading frames in antisense to annotated genes, which may constitute protein-coding genes. Read More

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December 2018

Urinary neutrophil gelatinase-associated lipocalin, kidney injury molecule-1, uromodulin, and cystatin C concentrations in an experimental rat model of ascending acute kidney injury induced by pyelonephritis.

J Physiol Pharmacol 2018 Aug 9;69(4). Epub 2018 Dec 9.

Department of Pathophysiology, Jagiellonian University Medical College, Cracow, Poland.

Recent evidence suggests that neutrophil gelatinase-associated lipocalin (NGAL), kidney injury molecule-1 (KIM-1), cystatin C (CysC), uromodulin (UMOD), and some interleukins (IL-6 and IL-18) can be considered as diagnostic markers of acute kidney injury (AKI). The aim of this study was to verify the applicability of four urinary (u) markers, namely uNGAL, uKIM-1, uCysC, and uUMOD, for the diagnosis of ascending AKI induced by bacterial pyelonephritis. The study included 30 female rats that were divided into three groups (n = 10 each) and were inoculated transurethrally with various doses of Escherichia coli to induce isolated pyelonephritis (group 1, 10 CFU/ml), pyelonephritis-induced AKI (group 2, 10 CFU/ml), or AKI and urosepsis (group 3, 10 CFU/ml). Read More

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Microbial patterns in patients with histamine intolerance.

J Physiol Pharmacol 2018 Aug 9;69(4). Epub 2018 Dec 9.

First Department of Medicine, Hector Center for Nutrition, Exercise and Sports, Friedrich-Alexander-Universitaet Erlangen-Nuernberg, Erlangen, Germany.

Histamine intolerance represents a controversially discussed disorder. Besides an impaired degradation of orally supplied histamine due to diamine oxidase (DAO) deficiency, a deranged gut flora may also contribute to elevated histamine levels. Our aim was to determine the intestinal bacterial composition in patients with proven histamine intolerance in comparison to other food intolerances and healthy controls. Read More

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Towards quantitative microbiome community profiling using internal standards.

Appl Environ Microbiol 2018 Dec 14. Epub 2018 Dec 14.

Division of Earth and Ocean Sciences, Nicholas School of the Environment, Duke University, Durham, NC 27708, USA

An inherent issue of high-throughput rRNA gene tag sequencing microbiome surveys is that they provide compositional data in relative abundances. This often leads to spurious correlations making the interpretation of relationships to biogeochemical rates challenging. To overcome this issue, we quantitatively estimated the abundance of microorganisms by spiking in known amounts of internal DNA standards. Read More

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December 2018

Molecular characterization of LysPBC2, a novel endolysin harboring a spore binding domain.

Appl Environ Microbiol 2018 Dec 14. Epub 2018 Dec 14.

Department of Food and Animal Biotechnology, Department of Agricultural Biotechnology, Research Institute of Agriculture and Life Sciences, and Center for Food and Bioconvergence, Seoul National University, Seoul 08826, South Korea

To control a spore-forming human pathogen , we isolated and characterized a novel endolysin, LysPBC2, from a newly isolated phage PBC2. Compared to the narrow host range of phage PBC2, LysPBC2 showed very broad lytic activity against all , , and species tested. In addition to a catalytic domain and a cell wall binding domain, LysPBC2 has a spore binding domain (SBD) partially overlapped with its catalytic domain, which specifically binds to spores but not to vegetative cells of Both immunogold electron microscopy and binding assay indicate that the SBD binds the external region of the spore cortex layer. Read More

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December 2018

survival duration in manure-amended soils is affected by spatiotemporal, agricultural, and weather factors: a multi-season, multi-site field study in the Mid-Atlantic U.S.

Appl Environ Microbiol 2018 Dec 14. Epub 2018 Dec 14.

University of Maryland Eastern Shore Department of Agriculture, Princess Anne, MD.

Untreated biological soil amendments of animal origin (BSAAO), like manure, are commonly used to fertilize soils for growing fruit and vegetable crops, and can contain enteric bacterial foodborne pathogens. Little is known about the comparative longitudinal survival of pathogens in agricultural fields containing different types of BSAAO, and field data may be useful to determine intervals between manure application and harvest of produce intended for human consumption to minimize foodborne illness. This study generated 324 survival profiles from 12 different field trials at three different sites (UMES, PA, BARC) in the Mid-Atlantic U. Read More

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December 2018

The ferredoxin-like protein, FerR, regulates PrbP activity in .

Appl Environ Microbiol 2018 Dec 14. Epub 2018 Dec 14.

Microbiology and Cell Science Department, Genetics Institute, Institute of Food and Agricultural Science, University of Florida, Gainesville, Florida, United States

In , PrbP is an important transcriptional accessory protein that regulates gene expression through interactions with the RNA polymerase β-subunit and a specific sequence on the promoter region. The constitutive expression of observed upon chemical inactivation of PrbP/DNA interactions indicated that the expression of was not auto-regulated at the level of transcription. This observation suggested that a modulatory mechanism via protein-protein interactions maybe involved. Read More

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December 2018

Population genetics and characterization of isolates in western jackdaws and game birds in Finland.

Appl Environ Microbiol 2018 Dec 14. Epub 2018 Dec 14.

Department of Food Hygiene and Environmental Health, Faculty of Veterinary Medicine, University of Helsinki, Helsinki, FI-00014, Finland

Poultry is considered a major reservoir and source of human campylobacteriosis but the roles of environmental reservoirs, including wild birds, have not been assessed in depth. In this study, we isolated and characterized from western jackdaws (n=91, 43%), mallard ducks (n=82, 76%) and pheasants (n=9, 9%). Most of the western jackdaw and mallard duck isolates represented MLST sequence types (STs) that diverged from those previously isolated from human patients and various animal species, whereas all pheasant isolates represented ST-19, a common ST among human patients and other hosts worldwide. Read More

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December 2018

A combination of linalool, vitamin C, and copper synergistically trigger Reactive Oxygen Species, DNA damage and inhibits subsp. serovar Typhi and .

Appl Environ Microbiol 2018 Dec 14. Epub 2018 Dec 14.

Chemical Biology Laboratory, Department of Biotechnology, Indian Institute of Technology Roorkee, Uttarakhand 247 667, India.

Inappropriate and disproportionate use of antibiotics is contributing immensely to the development of antibiotic resistance in bacterial species associated with food contamination. Use of natural products in combination can be a potent alternative hurdle strategy to inactivate foodborne pathogens. Here, we explored pro-oxidant properties of essential oil inalool and itamin C in combination with opper (LVC) in combating foodborne pathogens and a subsp. Read More

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December 2018

Galactose impacts the size and intracellular composition of the asaccharolytic oral pathobiont .

Appl Environ Microbiol 2018 Dec 14. Epub 2018 Dec 14.

Department of Oral Biology, College of Dentistry, University of Florida, Gainesville, Florida, USA

The asaccharolytic anaerobe metabolizes proteins it encounters in the periodontal pocket, including host-derived glycoproteins such as mucins and immunoglobulins. Often, these proteins are protected by a diverse array of carbohydrates tethered to the polypeptide chain via glycolytic bonds, and produces enzymes capable of liberating these carbohydrates, exposing the proteinaceous core. In this study, we investigated the effect of individual monosaccharides, including galactose, L-fucose, mannose and glucose, on the growth and physiology of Of the carbohydrates tested, only galactose noticeably altered the density of the bacterial culture, and we observed that cultures grown with galactose reached significantly higher densities during stationary phase. Read More

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December 2018

Structural basis for two efficient modes of agropinic acid opine import into the bacterial pathogen Agrobacterium tumefaciens.

Biochem J 2018 Dec 14. Epub 2018 Dec 14.

I2BC-CNRS, Gif sur Yvette, 91198, France

pathogens genetically modify their host plants to drive the synthesis of opines in plant tumors. The mannityl-opine family encompasses mannopine, mannopinic acid, agropine and agropinic acid. These opines serve as nutrients and are imported into bacteria periplasmic binding proteins (PBPs) in association with ABC transporters. Read More

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December 2018

Transcriptomic analyses uncover emerging roles of mucins, lysosome/secretory addressing and detoxification pathways in insect midguts.

Curr Opin Insect Sci 2018 Oct 5;29:34-40. Epub 2018 Jun 5.

Laboratório de Química e Função de Proteínas e Peptídeos, Centro de Biociências e Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, Campos dos Goytacazes, Brazil.

The study of insect midgut features has been made possible by the recent availability of transcriptome datasets. These data uncovered the preferential expression of mucus-forming mucins at midgut regions that require protection (e.g. Read More

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October 2018

Comparative study of cultures and next-generation sequencing in the diagnosis of shoulder prosthetic joint infections.

J Shoulder Elbow Surg 2019 Jan;28(1):1-8

Department of Orthopaedic Surgery, Rothman Institute-Thomas Jefferson University, Philadelphia, PA, USA.

Background: Serum and synovial markers used to diagnose lower extremity prosthetic joint infection (PJI) have performed poorly for shoulder PJI. As a result, diagnosis is commonly reliant on the accuracy of positive or negative cultures. Next-generation sequencing (NGS) can sequence an entire bacterial genome. Read More

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January 2019

Recent advances in antimicrobial and bacterial resistance.

Int J Antimicrob Agents 2018 Dec;52(6):869-870

Laboratoire Neurophysiologie, Physiopathologie Cellulaire et Valorisation des BioMolécules, Faculté des Sciences de Tunis, Université de Tunis El-Manar, Campus Universitaire, 2098 El-Manar II, Tunisia.

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December 2018

Clinical, immunological and bacteriological characteristics of H7N9 patients nosocomially co-infected by Acinetobacter Baumannii: a case control study.

BMC Infect Dis 2018 Dec 14;18(1):664. Epub 2018 Dec 14.

Shenzhen Key Laboratory of Pathogen and Immunity, State Key Discipline of Infectious Disease, Shenzhen Third People's Hospital, Shenzhen, 518112, China.

Background: Bacterial co-infection of patients suffering from influenza pneumonia is a key element that increases morbidity and mortality. The occurrence of Acinetobacter baumannii co-infection in patients with avian influenza A (H7N9) virus infection has been described as one of the most prevalent bacterial co-infections. However, the clinical and laboratory features of this entity of H7N9 and A. Read More

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December 2018

Geometric Effect for Biological Reactors and Biological Fluids.

Bioengineering (Basel) 2018 Dec 13;5(4). Epub 2018 Dec 13.

Department of Physics, Kyushu University, Fukuoka 819-0395, Japan.

As expressed "God made the bulk; the surface was invented by the devil" by W. Pauli, the surface has remarkable properties because broken symmetry in surface alters the material properties. In biological systems, the smallest functional and structural unit, which has a functional bulk space enclosed by a thin interface, is a cell. Read More

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December 2018

Impact of Rearing Conditions on the Ambrosia Beetle's Microbiome.

Life (Basel) 2018 Dec 13;8(4). Epub 2018 Dec 13.

Red de Estudios Moleculares Avanzados, Instituto de Ecología A. C., Xalapa C.P. 91070, Mexico.

Ambrosia beetles, along with termites and leafcutter ants, are the only fungus-farming lineages within the tree of life. Bacteria harbored by ambrosia beetles may play an essential role in the nutritional symbiotic interactions with their associated fungi; however, little is known about the impact of rearing conditions on the microbiota of ambrosia beetles. We have used culture-independent methods to explore the effect of rearing conditions on the microbiome associated with , , and , evaluating different media in laboratory-controlled conditions and comparing wild and laboratory conditions. Read More

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December 2018

Iron Biofortified Carioca Bean ( L.)-Based Brazilian Diet Delivers More Absorbable Iron and Affects the Gut Microbiota In Vivo ().

Nutrients 2018 Dec 13;10(12). Epub 2018 Dec 13.

USDA-ARS, Robert W. Holley Center for Agriculture and Health, Cornell University, Ithaca, NY 14850, USA.

Biofortification aims to improve the micronutrient concentration and bioavailability in staple food crops. Unlike other strategies utilized to alleviate Fe deficiency, studies of the gut microbiota in the context of Fe biofortification are scarce. In this study, we performed a 6-week feeding trial in ( = 15), aimed to investigate the Fe status and the alterations in the gut microbiome following the administration of Fe-biofortified carioca bean based diet (BC) versus a Fe-standard carioca bean based diet (SC). Read More

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December 2018

Azalomycin F, a polyhydroxy macrolide binding to the polar head of phospholipid and targeting to lipoteichoic acid to kill methicillin-resistant Staphylococcus aureus.

Biomed Pharmacother 2019 Jan 26;109:1940-1950. Epub 2018 Nov 26.

College of Bioscience and Bioengineering, Jiangxi Agricultural University, Nanchang 330045, China.

Azalomycin F was a polyhydroxy macrolide produced by streptomycete strains. Our preliminary researches indicated that it could kill methicillin-resistant Staphylococcus aureus (MRSA) likely by increasing the permeability of cell membrane, and that cell-membrane phospholipids were likely important targets. To confirm this, membrane permeability assay was performed and visualized by fluorescence staining, and then the detailed interactions between azalomycin F and model membranes prepared with 1,2-dihexadecanoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (DPPG) were determined using attenuated total reflectance fourier transform infrared spectroscopy and P nuclear magnetic resonance techniques. Read More

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January 2019

The protective role of Piper nigrum fruit extract in an ovalbumin-induced allergic rhinitis by targeting of NFκBp65 and STAT3 signalings.

Biomed Pharmacother 2019 Jan 26;109:1915-1923. Epub 2018 Nov 26.

Department of Anatomy, Chonbuk National University Medical School, Jeonju, Jeonbuk 54896, Republic of Korea; Institute for Medical Sciences, Chonbuk National University, Jeonju, Jeonbuk 54896, Republic of Korea. Electronic address:

Piper nigrum L. is commonly used as a traditional medicine and food in many countries. It has been reported to have anti-oxidant, anti-bacterial, anti-tumor, anti-mutagenic, anti-diabetic, and anti-inflammatory properties. Read More

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January 2019

A review on mechanism of action, resistance, synergism, and clinical implications of mupirocin against Staphylococcus aureus.

Biomed Pharmacother 2019 Jan 26;109:1809-1818. Epub 2018 Nov 26.

Department of Microbiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran; Student Research Committee, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

Mupirocin (MUP), bactroban, or pseudomonic acid is a natural crotonic acid derivative drug extracted from Pseudomonas fluorescens which is produced by modular polyketide synthases. This antibiotic has a unique chemical structure and mechanism of action. It is a mixture of A-D pseudomonic acids and inhibits protein synthesis through binding to bacterial isoleucyl-tRNA synthetase. Read More

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January 2019
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Effectiveness of topical administration of Anethum graveolens essential oil on MRSA-infected wounds.

Biomed Pharmacother 2019 Jan 17;109:1650-1658. Epub 2018 Nov 17.

Department of Biology, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, G.C. Evin, Tehran, Iran.

Medicinal plants are conventionally used for wound healing, but their action mechanisms are still unknown. The present study evaluated the effect of topical administration of ointment containing dill (Anethum graveolens) essential oil (DEO) in the management of apoptosis and cell proliferation during MRSA-infected experimentally induced wound healing process in BALB/c mice model. The GC-FID and GC-MS techniques were used to analyze chemical composition of the essential oil. Read More

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January 2019
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