10,779 results match your criteria Bacillary Angiomatosis


Identification of risk factors and diagnostic methodology in serositis by M. tuberculosis

Rev Med Inst Mex Seguro Soc 2018 11 30;56(4):364-370. Epub 2018 Nov 30.

Secretaría de Salud, Hospital General de México “Dr. Eduardo Liceaga”, Unidad de Neumología y Cirugía de Tórax. Ciudad de México, México

Background: Tuberculosis is a public health problem, extrapulmonary presentations have increased, it is difficult to diagnose because of the low bacillary load.

Objective: To identify risk factors and to evaluate the efficiency of diagnostic methods in pleural, meningeal, peritoneal and pericardial tuberculosis.

Methods: Prospective study of cases and controls. Read More

View Article
November 2018

New CRISPR-Cas9 vectors for genetic modifications of Bacillus species.

FEMS Microbiol Lett 2018 Dec 6. Epub 2018 Dec 6.

Institut für Industrielle Genetik, Universität Stuttgart, Allmandring 31, 70569 Stuttgart, Germany.

Genetic manipulation is a fundamental procedure for the study of gene and operon functions and new characteristics acquisition. Modern CRISPR-Cas technology allows genome editing more precise and increases the efficiency of transferring mutations in a variety of hard to manipulate organisms. Here, we describe new CRISPR-Cas vectors for genetic modifications in bacillary species. Read More

View Article
December 2018
1 Read

Bartonella infections in cats and dogs including zoonotic aspects.

Parasit Vectors 2018 Dec 4;11(1):624. Epub 2018 Dec 4.

Departament de Medicina i Cirurgia Animals, Facultat de Veterinària, Universitat Autònoma de Barcelona, 08193, Bellaterra, Spain.

Bartonellosis is a vector-borne zoonotic disease with worldwide distribution that can infect humans and a large number of mammals including small companion animals (cats and dogs). In recent years, an increasing number of studies from around the world have reported Bartonella infections, although publications have predominantly focused on the North American perspective. Currently, clinico-pathological data from Europe are more limited, suggesting that bartonellosis may be an infrequent or underdiagnosed infectious disease in cats and dogs. Read More

View Article
December 2018

Transformation Morphisms and Time-to-Extinction Analysis That Map Therapy Duration From Preclinical Models to Patients With Tuberculosis: Translating From Apples to Oranges.

Clin Infect Dis 2018 Nov;67(suppl_3):S349-S358

Center for Infectious Diseases Research and Experimental Therapeutics, Baylor Research Institute, Dallas, Texas.

Background: A major challenge in medicine is translation of preclinical model findings to humans, especially therapy duration. One major example is recent shorter-duration therapy regimen failures in tuberculosis.

Methods: We used set theory mapping to develop a computational/modeling framework to map the time it takes to extinguish the Mycobacterium tuberculosis population on chemotherapy from multiple hollow fiber system model of tuberculosis (HFS-TB) experiments to that observed in patients. Read More

View Article
November 2018
1 Read

Regulatory Hierarchies Controlling Virulence Gene Expression in and .

Front Microbiol 2018 9;9:2686. Epub 2018 Nov 9.

Department of Microbiology, Moyne Institute of Preventive Medicine, Trinity College Dublin, Dublin, Ireland.

Gram-negative enteropathogenic bacteria use a variety of strategies to cause disease in the human host and gene regulation in some form is typically a part of the strategy. This article will compare the toxin-based infection strategy used by the non-invasive pathogen , the etiological agent in human cholera, with the invasive approach used by , the cause of bacillary dysentery. Despite the differences in the mechanisms by which the two pathogens cause disease, they use environmentally-responsive regulatory hierarchies to control the expression of genes that have some features, and even some components, in common. Read More

View Article
November 2018
3 Reads

Intrapulmonary pharmacokinetics of antibiotics used to treat nosocomial pneumonia caused by gram-negative bacilli: a systematic review.

Int J Antimicrob Agents 2018 Nov 22. Epub 2018 Nov 22.

Centre for Translational Anti-Infective Pharmacodynamics, School of Pharmacy, The University of Queensland, Woolloongabba, Queensland, Australia; Faculty of Medicine, The University of Queensland, Brisbane, Queensland 4006, Australia.

Background: . Knowledge of antibiotic concentrations achievable in the epithelial lining fluid (ELF) will help guide antibiotic dosing for treating patients with gram-negative bacillary ventilator-associated pneumonia (VAP).

Objective: . Read More

View Article
November 2018
4 Reads

Nematode capilaridae in the tongue of Cerdocyon thous of free life in Brazil.

Rev Bras Parasitol Vet 2018 Oct-Dec;27(4):531-544. Epub 2018 Nov 8.

Laboratório de Histologia e Embriologia Animal, Instituto da Saúde e Produção Animal, Universidade Federal Rural da Amazônia - UFRA, Belém, PA, Brasil.

Studies of helminths in road-killed wild animals are still uncommon but may provide promising results since they can identify the parasites in juvenile and adult hosts and meet the recommendations of current discussions on bioethics that prioritize alternative methods for the use of animals. This study evaluated three Cerdocyon thous individuals that were donated after dying from being run over. Two of them had small nematode adults in the epithelial and connective tissues of the tongue. Read More

View Article
November 2018

Relationship between chest radiographic characteristics, sputum bacterial load, and treatment outcomes in patients with extensively drug-resistant tuberculosis.

Int J Infect Dis 2018 Nov 2. Epub 2018 Nov 2.

Division of Pulmonology and UCT Lung Institute, Department of Medicine, University of Cape Town, South Africa. Electronic address:

Background: Data about the relationship between chest radiographs and sputum bacillary load, with treatment outcomes, in patients with extensively drug-resistant tuberculosis (XDR-TB) from HIV/TB endemic settings are limited.

Methods: Available chest radiographs from 97 South African XDR-TB patients, at the time of diagnosis, were evaluated by two independent readers using a validated scoring system. Chest radiograph findings were correlated with baseline sputum bacillary load (smear-grade and culture time-to-positive in MGIT), and prospectively ascertained clinical outcomes (culture conversion and all-cause mortality). Read More

View Article
November 2018
2 Reads

A Case of Generalized, Superinfected Dermatitis and Inguinal Mycobacterium Lymphadenitis - TB or not TB?

Acta Dermatovenerol Croat 2018 Oct;26(3):270-272

Gyula Laszlo Fekete, MD, Bdul. 1 Decembrie 1918, no. 211/27, Tg. Mures, Romania:

Dear Editor, Eczema is an inflammatory dermatitis mediated by cellular immunity, with an etiology in which environmental, immunological, and genetic factors are involved. Skin inflammation through proinflammatory cytokines creates a favorable environment for microbial antigens and optimal conditions for infection (1). In case of underlying immunosuppression, inflammatory features of dermatitis and superimposed infections are more severe. Read More

View Article
October 2018
8 Reads

Bacterial contamination and health risks of drinking water from the municipal non-government managed water treatment plants.

Environ Monit Assess 2018 Oct 29;190(11):685. Epub 2018 Oct 29.

Botany and Microbiology Department, Faculty of Science, Cairo University, Giza, 12613, Egypt.

Water quality and bacterial contamination from 18 drinking water municipal plants in three locations at Giza governorate were investigated. The average total count of bacteria detected after four stages of treatments in the investigated plants was 32 CFU/1 mL compared to 2330 cfu/mL for raw water, with a reduction percentage of 98.6. Read More

View Article
October 2018
5 Reads
1.680 Impact Factor

Effect of ambient temperature and its effect modifiers on bacillary dysentery in Jinan, China.

Sci Total Environ 2019 Feb 5;650(Pt 2):2980-2986. Epub 2018 Oct 5.

Department of Epidemiology, School of Public Health, Shandong University, Jinan 250012, Shandong, China. Electronic address:

Background: Studies about the modified effect among various meteorological factors on bacillary dysentery (BD) are limited. This study aimed to investigate the effect of ambient temperature and its effect modifiers on BD in Jinan.

Methods: Daily data of BD cases and meteorological factors from 2005 to 2013 were collected. Read More

View Article
February 2019
2 Reads

[Distributed lag effects on the relationship between daily mean temperature and the incidence of bacillary dysentery in Lanzhou city].

Beijing Da Xue Xue Bao Yi Xue Ban 2018 Oct;50(5):861-867

School of Public Health, Lanzhou University, Lanzhou 730000, China.

Objective: To discuss the lag effects of daily average temperature on the daily cases of bacillary dysentery in Lanzhou city.

Methods: The data of daily cases of bacillary dysentery were collected during 2008 and 2015 in the city, and the meteorological data at the same period was integrated. The distributed lag non-linear model was used to analyze the relevance between daily average temperature and the daily cases of bacillary dysentery. Read More

View Article
October 2018
5 Reads

Rv3033, as an Emerging Anti-apoptosis Factor, Facilitates Mycobacteria Survival via Inhibiting Macrophage Intrinsic Apoptosis.

Front Immunol 2018 21;9:2136. Epub 2018 Sep 21.

Jiangsu Key Laboratory of Infection and Immunity, Institutes of Biology and Medical Sciences, Soochow University, Suzhou, China.

Apoptosis inhibition is a critical strategy of mycobacteria facilitating its survival in macrophages, but the underlying mechanism is not completely understood. In this study, we found that Rv3033, a secreted virulence factor of mycobacteria, played an important role in bacillary survival within macrophages. Forced over-expressed of Rv3033 in macrophages could efficiently resist mycobacteria-induced early and late apoptosis, accompanied with the obvious increased cellular bacterial burden. Read More

View Article
September 2018
1 Read

Bartonella henselae in a dog with ear tip vasculitis.

Vet Dermatol 2018 Dec 14;29(6):537-e180. Epub 2018 Oct 14.

Intracellular Pathogens Research Laboratory, Comparative Medicine Institute, College of Veterinary Medicine, North Carolina State University, 1050 William Moore Drive, Raleigh, NC, 27607, USA.

Background: Bartonella henselae, a Gram-negative, zoonotic, alpha-proteobacteria has been previously implicated in association with cutaneous vasoproliferative lesions (bacillary angiomatosis), nodular panniculitis and multifocal erythema (erythema multiforme) in dogs.

Objective: Describe clinical, microbiological and histological lesions in a dog with ear margin vasculitis and B. henselae infection. Read More

View Article
December 2018
1 Read

Pre-treatment cerebrospinal fluid bacterial load correlates with inflammatory response and predicts neurological events during tuberculous meningitis treatment.

J Infect Dis 2018 Oct 9. Epub 2018 Oct 9.

Oxford University Clinical Research Unit, Ho Chi Minh City, Viet Nam.

Background: Mycobacterium tuberculosis (Mtb) bacillary load in the brain of those with tuberculous meningitis (TBM) may reflect the host ability to control the pathogen and determine disease severity and treatment outcomes.

Methods: We measured pre-treatment cerebrospinal fluid (CSF) Mtb bacterial load by GeneXpert in 692 adults with TBM. We sought to understand the relationship between CSF bacterial load and inflammation, and their respective impact on disease severity and treatment outcomes. Read More

View Article
October 2018
6 Reads

Community-acquired meningitis with ventriculitis in an adult-a rare case report.

J Intensive Care 2018 24;6:63. Epub 2018 Sep 24.

Department of Microbiology, Yashoda Hospitals, Secunderabad, India.

Background: Community-acquired gram-negative bacillary meningitis is rare to occur without preexisting conditions like trauma, organ dysfunction, and immunocompromised state, and very few case reports with have been described in literature till now. Presence of ventriculitis along with meningitis makes the incidence further sparse.

Case Presentation: A review of literature identified a total of only 45 community-acquired meningitis from 1945 till to date. Read More

View Article
September 2018

Immunological correlates of mycobacterial growth inhibition describe a spectrum of tuberculosis infection.

Sci Rep 2018 Sep 27;8(1):14480. Epub 2018 Sep 27.

The Jenner Institute, Nuffield Department of Medicine, University of Oxford, Oxford, UK.

A major contribution to the burden of Tuberculosis (TB) comes from latent Mycobacterium tuberculosis infections (LTBI) becoming clinically active. TB and LTBI probably exist as a spectrum and currently there are no correlates available to identify individuals with LTBI most at risk of developing active disease. We set out to identify immune parameters associated with ex vivo mycobacterial growth control among individuals with active TB disease or LTBI to define the spectrum of TB infection. Read More

View Article
September 2018
9 Reads

An outbreak of Shigella boydii serotype 20 in January 2015 amongst United Kingdom healthcare workers involved in the Ebola response in Sierra Leone.

J Med Microbiol 2018 Nov 25;67(11):1596-1600. Epub 2018 Sep 25.

1​Field Epidemiology Services, National Infection Service, Public Health England, London, UK.

In January 2015, Public Health England and the United Kingdom (UK) Ministry of Defence investigated cases of diarrhoea and fever in military personnel recently returned to the UK after supporting the response to the Ebola epidemic in Sierra Leone. Tests for Ebola virus infection were negative. PCR tests detected the ipaH gene in 10/12 faecal specimens, and Shigella boydii serotype 20 was isolated from 7 patients. Read More

View Article
November 2018
1 Read

Rv2346c enhances mycobacterial survival within macrophages by inhibiting TNF-α and IL-6 production via the p38/miRNA/NF-κB pathway.

Emerg Microbes Infect 2018 Sep 19;7(1):158. Epub 2018 Sep 19.

Department of Respiratory Medicine, the Second Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, 210011, China.

The intracellular survival of Mycobacterium tuberculosis (Mtb) has a central role in the pathogenesis of tuberculosis. Mtb Rv2346c is a member of 6-kDa early secreted antigenic target family of proteins, which are known to inhibit the host immune responses to promote bacillary persistence in macrophages. However, the mechanism through which Rv2346c participates in Mtb pathogenesis is unclear. Read More

View Article
September 2018
7 Reads

Shigella and Enteroinvasive Escherichia Coli.

Curr Top Microbiol Immunol 2018 Sep 15. Epub 2018 Sep 15.

Microbiologie et Maladies Infectieuses, Collège de France, 11 Place Marcelin Berthelot, 75005, Paris, France.

Shigella and enteroinvasive Escherichia coli (EIEC) are gram-negative bacteria responsible for bacillary dysentery (shigellosis) in humans, which is characterized by invasion and inflammatory destruction of the human colonic epithelium. Different EIEC and Shigella subgroups rose independently from commensal E. coli through patho-adaptive evolution that included loss of functional genes interfering with the virulence and/or with the intracellular lifestyle of the bacteria, as well as acquisition of genetic elements harboring virulence genes. Read More

View Article
September 2018
10 Reads

Mycobacterium tuberculosis Joyrides Alveolar Macrophages into the Pulmonary Interstitium.

Cell Host Microbe 2018 09;24(3):331-333

Lydia Becker Institute of Infection and Immunology, University of Manchester, Manchester, UK. Electronic address:

Alveolar macrophages reside in the airway lumen, where they are thought to remain. However, in this issue of Cell Host & Microbe,Cohen et al. (2018) show that Mycobacterium tuberculosis may induce their translocation into the lung interstitium, potentially acting as a Trojan horse for bacillary dissemination to other phagocytes. Read More

View Article
September 2018

Prevalence of enterotoxin-encoding genes among diverse Shigella strains isolated from patients with diarrhea, southwest Iran.

Acta Microbiol Immunol Hung 2018 Sep 11:1-11. Epub 2018 Sep 11.

4 Department of Laboratory Sciences, Golestan Hospital, Jundishapur University of Medical Sciences , Ahvaz, Iran.

Shigella spp. are a major cause of bacillary dysentery, particularly among children in developing countries such as Iran. This study aimed to investigate the presence of two important Shigella enterotoxins (ShET-1 and ShET-2), encoded by the set and sen genes, respectively, by polymerase chain reaction (PCR) assay among Shigella species isolated from children affected by shigellosis in Ahvaz, southwest of Iran. Read More

View Article
September 2018
2 Reads

[The Analysis of Morbidity of Tuberculosis of Respiratory Organs of Women of Reproductive Age on the Territory of the Nizhny Novgorod Oblast].

Authors:
G F Rusanovskaya

Probl Sotsialnoi Gig Zdravookhranenniiai Istor Med 2018 May;26(3):141-143

The State Budget Educational Institution of Higher Professional Education "The Nizhny Novgorod State Medical Academy" of Minzdrav of Russia, Nizhny Novgorod, 603005, Russia,

The article presents analysis of epidemiological situation with tuberculosis of respiratory organs among women of reproductive age on the territory of the Nizhny Novgorod oblast in 2009-2013. The average age of women with primarily diagnosed tuberculosis of respiratory organs was 38.8 years. Read More

View Article

Longitudinal analysis of serum cytokine profile among patients with tubercular multifocal serpiginoid choroiditis: a pilot study.

Eye (Lond) 2018 Sep 5. Epub 2018 Sep 5.

Advanced Eye Center, Post Graduate Institute of Medical Education and Research, Sector 12, Chandigarh, 160012, India.

Purpose: To analyze the serum cytokines profile in patients with tubercular multifocal serpiginoid choroiditis (TB MSC) receiving anti-tubercular therapy (ATT) and oral corticosteroids.

Methods: In this prospective longitudinal study, patients with active TB MSC were included. Serum levels of interferon (IFN)-γ, interleukin (IL)-10, and tumor necrosis factor (TNF)-α were analyzed using bead-based immunoassay. Read More

View Article
September 2018
1 Read
1.900 Impact Factor

[Mycobacteria identification in solid culture by inverted phase microscopy and ziehl-neelsen stain].

Rev Peru Med Exp Salud Publica 2018 Apr-Jun;35(2):279-284

Universidad Peruana Cayetano Heredia, Instituto de Enfermedades Tropicales Alexander von Humboldt. Lima, Perú.

With the aim of identifying morphological differences in colonies of different species of mycobacteria by means of inverted phase microscopy, nine species of high-prevalence pathogenic mycobacteria were seeded in 7H11 solid medium and were observed for up to 21 days. Additionally, Ziehl-Neelsen (ZN) stains were carried out for each one of them. No morphological variations were identified among the species grown on solid medium; however, it was identified that all are able to form cords in solid medium in an early stage of growth, being corroborated by the Ziehl-Neelsen stain in Mycobacterium tuberculosis. Read More

View Article
February 2018

Evaluation of Auramine O staining and conventional PCR for leprosy diagnosis: A comparative cross-sectional study from Ethiopia.

PLoS Negl Trop Dis 2018 Sep 4;12(9):e0006706. Epub 2018 Sep 4.

Armauer Hansen Research Institute, Addis Ababa, Ethiopia.

Background: Diagnosis of leprosy mainly relies on clinical examination due to the inconsistent sensitivity and poor reproducibility of the current laboratory tests. Utilisation of alternative methods to the standard Ziehl Neelsen (ZN), Fite-Faraco (FF) and Haematoxylin and Eosin (H&E) staining procedures may eventually improve leprosy diagnosis.

Methodology/principal Findings: In this comparative study, the performance of the fluorescent Auramine O (AO) staining and polymerase chain reaction (PCR) was assessed with different skin samples using a combination of ZN, FF and H&E staining as the gold standard. Read More

View Article
September 2018
2 Reads

Spatiotemporal Characteristics of Bacillary Dysentery from 2005 to 2017 in Zhejiang Province, China.

Int J Environ Res Public Health 2018 08 24;15(9). Epub 2018 Aug 24.

Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China.

Background: This study aimed to analyze the epidemiological and spatiotemporal characteristics of bacillary dysentery in Zhejiang Province and to provide the basis for its monitoring, prevention and control.

Methods: This study included cases registered in China Information System for Diseases Control and Prevention from 1 January 2005 to 31 December 2017 in Zhejiang. Descriptive methods were employed to investigate the long trend of this disease: gender distribution, high-risk population, seasonality, and circular distribution was explored to detect the peak period; incidence maps were made to show the incidence trend of disease at county level; spatial autocorrelation was explored and the regions with autocorrelation were detected; and spatiotemporal scan was conducted to map out the high-risk regions of disease and how long they lasted. Read More

View Article
August 2018
1 Read

PRESUMED FOVEAL BACILLARY LAYER DETACHMENT IN A PATIENT WITH TOXOPLASMOSIS CHORIORETINITIS AND PACHYCHOROID DISEASE.

Retin Cases Brief Rep 2018 Aug 23. Epub 2018 Aug 23.

Department of Ophthalmology, New York University, New York, New York.

Purpose: To report a detachment that apparently separated photoreceptor inner segment myoids from inner segment ellipsoids as a manifestation of toxoplasmosis chorioretinitis in a patient with pachychoroid spectrum disease.

Methods: Multimodal imaging including fundus photography, spectral domain and enhanced-depth imaging optical coherence tomography (OCT), indocyanine green angiography, and OCT angiography.

Results: A 33-year-old man with a history of toxoplasmosis chorioretinitis reported 1 week of decreased vision to 20/200 in his right eye. Read More

View Article

Adjuvant Potential of Poly-α-l-Glutamine from the Cell Wall of Mycobacterium tuberculosis.

Infect Immun 2018 Oct 21;86(10). Epub 2018 Sep 21.

Molecular and Cell Biology Laboratory, School of Biotechnology, Jawaharlal Nehru University, New Delhi, India

Novel adjuvants are in demand for improving the efficacy of human vaccines. The immunomodulatory properties of cell wall components have been highlighted in the formulation of complete Freund's adjuvant (CFA). We have explored the adjuvant potential of poly-α-l-glutamine (PLG), a lesser-known constituent of the pathogenic mycobacterial cell wall. Read More

View Article
October 2018
3 Reads
3.730 Impact Factor

Fixed-dose combination associated with faster time to smear conversion compared to separate tablets of anti-tuberculosis drugs in patients with poorly controlled diabetes and pulmonary tuberculosis in Qatar.

BMC Infect Dis 2018 Aug 8;18(1):384. Epub 2018 Aug 8.

Division of Infectious Diseases, School of Medicine, University of Virginia, Charlottesville, VA, 22908, USA.

Background: Diabetes is associated with increased risk of tuberculosis (TB) treatment failure, death, and relapse compared to patients without diabetes. Current TB regimens are available as fixed dose combination (FDC) and separate tablets (ST), in which using the former is purported to make it easier to adhere and complete treatment. So far there are no studies assessing the performance of FDC compared to ST in diabetic patients with pulmonary TB. Read More

View Article
August 2018
5 Reads
2.610 Impact Factor

Distribution and characterization of Shiga toxin converting temperate phages carried by Shigella flexneri in Hispaniola.

Infect Genet Evol 2018 Nov 31;65:321-328. Epub 2018 Jul 31.

Emerging Pathogens Institute, University of Florida, Gainesville, FL, USA; Department of Environmental and Global Health, University of Florida, Gainesville, FL, USA. Electronic address:

Shigella infections account for a considerable burden of acute diarrheal diseases worldwide and remain a major cause of childhood mortality in developing countries. Although, all four species of Shigella (S. dysenteriae, S. Read More

View Article
November 2018
7 Reads

Cat-Scratch Disease in an AIDS Patient Presenting with Generalized Lymphadenopathy: An Unusual Presentation with Delayed Diagnosis.

Am J Case Rep 2018 Aug 2;19:906-911. Epub 2018 Aug 2.

Department of Internal Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, USA.

BACKGROUND Bartonella infection is the causative organism of cat-scratch disease (CSD), which typically presents with self-limited localized lymphadenopathy. In HIV-infected patients, Bartonella infection can cause systemic illnesses with significant morbidity and mortality manifesting as bacillary angiomatosis (BA), hepatic peliosis, splenitis, bacteremic febrile illness, and other organ involvement. To the best of our knowledge, there have been no reports of HIV-infected patients presenting with generalized lymphadenopathy caused by Bartonella infection. Read More

View Article
August 2018
2 Reads

A Novel Proteome Microarray Discriminates Targets of Human Antibody Reactivity following Oral Vaccination and Experimental Challenge.

mSphere 2018 08 1;3(4). Epub 2018 Aug 1.

Center for Vaccine Development and Global Health, University of Maryland School of Medicine, Baltimore, Maryland, USA

spp. are a major cause of diarrhea and dysentery in children under 5 years old in the developing world. The development of an effective vaccine remains a public health priority, necessitating improved understanding of immune responses to and identification of protective antigens. Read More

View Article
August 2018
10 Reads

Characterization of New Leprosy Cases in Northeast of Iran within the Last 15 Years.

Iran J Med Sci 2018 Jul;43(4):416-420

Antimicrobial Resistance Research Center, Buali Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran.

Leprosy is a neglected disease. The insidious onset of leprosy results in its late diagnosis and therefore the spread of the disease. Characterization of leprosy in areas with higher risk and collection of enough information about these spots to adopt more effective controlling measures are of great importance. Read More

View Article
July 2018
8 Reads

Comparison of the Purelyse® - IS6110 nested PCR with the Xpert® MTB/RIF test in clinical samples with suspected tuberculosis.

J Microbiol Methods 2018 Sep 20;152:48-51. Epub 2018 Jul 20.

Laboratorio de Patogénesis Molecular, Facultad de Medicina Mexicali, Universidad Autónoma de Baja California, Mexico. Electronic address:

Tuberculosis (TB) has a high incidence, prevalence and mortality in the world. Due to its high level of transmission and long-term pharmacological treatment, it is important to have sensitive and specific diagnostic tests. Recently, the PureLyse® system, which is a novel DNA extraction method, was proposed to be an important tool for molecular diagnosis of TB. Read More

View Article
September 2018
1 Read

Toll-Like Receptor-1 Single-Nucleotide Polymorphism 1805T/G Is Associated With Predisposition to Multibacillary Tuberculosis.

Front Immunol 2018 25;9:1455. Epub 2018 Jun 25.

Programa de Pós-Graduação em Imunologia Básica e Aplicada, Universidade Federal do Amazonas, Manaus, Brazil.

Tuberculosis (TB), caused by mycobacterial species of the complex, is a serious global health issue. Brazil is among the 22 countries with the highest number of TB cases, and the state of Amazonas has the highest incidence of TB cases in the country. Toll-like receptors (TLRs) are important pattern recognition receptors of the innate immunity and play a key role in orchestrating an effective immune response. Read More

View Article

Isolation and Antimicrobial Susceptibility Profile of Shigella and Salmonella Species from Children with Acute Diarrhoea in Mekelle Hospital and Semen Health Center, Ethiopia.

Ethiop J Health Sci 2018 Mar;28(2):197-206

Department of Microbiology, Immunology and Parasitology, School of Medicine, College of Health Sciences, Addis Ababa University.

Background: Salmonella and Shigella remain the major contributors to acute enteric infections and diarrhoea. Hence, the objective of this study was to isolate and determine the antimicrobial susceptibility pattern of Shigella and Salmonella species from children with acute diarrhoea in Mekelle Hospital and Semen Health Center.

Methods: A cross sectional study was conducted among 260 children with acute diarrhoea from November 2011 to March 2012 in Mekelle, Ethiopia. Read More

View Article
March 2018
7 Reads

Comparison of Löwenstein-Jensen and BACTEC MGIT 960 culture for Mycobacterium tuberculosis in people living with HIV.

HIV Med 2018 Oct 3;19(9):654-661. Epub 2018 Jul 3.

Infectious Diseases Institute, College of Health Sciences, Makerere University, Kampala, Uganda.

Objectives: The aim of the study was to clarify how HIV infection affects tuberculosis liquid and solid culture results in a resource-limited setting.

Methods: We used baseline data from the Study on Outcomes Related to Tuberculosis and HIV Drug Concentrations in Uganda (SOUTH), which included 268 HIV/tuberculosis (TB)-coinfected individuals. Culture results from Löwenstein-Jensen (LJ) solid culture and mycobacteria growth indicator tube (MGIT) liquid culture systems and culture-based correlates for bacillary density from the sputum of HIV/TB-coinfected individuals at baseline were analysed. Read More

View Article
October 2018
1 Read

Does discovery of differentially culturable M tuberculosis really demand a new treatment paradigm? Longitudinal analysis of DNA clearance from sputum.

BMC Infect Dis 2018 Jul 3;18(1):293. Epub 2018 Jul 3.

Yale School of Public Health, New Haven, CT, USA.

Background: According to the traditional tuberculosis (TB) treatment paradigm, the initial doses of treatment rapidly kill most Mycobacterium tuberculosis (Mtb) bacilli in sputum, yet many more months of daily treatment are required to eliminate a small, residual subpopulation of drug-tolerant bacilli. This paradigm has recently been challenged following the discovery that up to 90% of Mtb bacilli in sputum are culturable only with growth-factor supplementation. These "differentially culturable" bacilli are hypothesized to be more drug-tolerant than routinely culturable bacilli. Read More

View Article
July 2018
12 Reads

Novel multiplex PCR assay for identification and subtyping of enteroinvasive Escherichia coli and differentiation from Shigella based on target genes selected by comparative genomics.

J Med Microbiol 2018 Sep 3;67(9):1257-1264. Epub 2018 Jul 3.

1​Centre for Infectious Diseases and Microbiology - Public Health, Institute of Clinical Pathology and Medical Research - NSW Health Pathology, Westmead Hospital, Westmead, New South Wales, Australia.

Purpose: Both Shigella and enteroinvasive Escherichia coli (EIEC) can cause enterocolitis, but they have a distinct epidemiology and public health relevance. Current culture-independent testing (CIT) methods to identify Shigella in faecal samples rely on the ipaH gene as the target, which is also found in EIEC genomes. The aim of this study was to design an assay that can identify EIEC in cultures from CIT ipaH-positive samples. Read More

View Article
September 2018
4 Reads

Cyclic OmpC peptidic epitope conjugated to tetanus toxoid as a potential vaccine candidate against shigellosis.

Vaccine 2018 07 28;36(31):4641-4649. Epub 2018 Jun 28.

Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland; Wroclaw Research Center EIT+, Wroclaw, Poland.

In earlier works we have described that mice immunized with outer membrane protein OmpC survive the challenge with live Shigella flexnerii 3a. We have also identified conformational epitope of this protein, that was recognized by mice antibodies. The aim of current work was to investigate whether synthetic OmpC epitope homologs can elicit immunological response sufficient in protecting mice against shigellosis. Read More

View Article
July 2018
6 Reads

Antimicrobial resistance profiles of Shigella dysenteriae isolated from travellers returning to the UK, 2004-2017.

J Med Microbiol 2018 Aug 29;67(8):1022-1030. Epub 2018 Jun 29.

Gastrointestinal Bacteria Reference Unit, Public Health England, Colindale, London, UK.

Purpose: Antimicrobial resistance (AMR) profiles of 754 strains of Shigella dysenteriae isolated between 2004 and 2017 from UK travellers reporting symptoms of gastrointestinal (GI) disease were reviewed to look for evidence of emerging AMR associated with travellers' diarrhoea.

Methodology: A travel history was provided for 72.7 % (548/754) of cases, of which 90. Read More

View Article
August 2018
3 Reads

Evaluation of the Xpert MTB/RIF Ultra Assay for Direct Detection of Mycobacterium tuberculosis Complex in Smear-Negative Extrapulmonary Samples.

J Clin Microbiol 2018 Sep 27;56(9). Epub 2018 Aug 27.

Department of Microbiology, Hospital Universitari de Bellvitge-IDIBELL, Hospitalet de Llobregat, Spain

The rapid detection of complex (MTUBC) in clinical samples is essential for successful treatment. New techniques such as real-time PCR have been developed in order to facilitate rapid diagnosis, but their sensitivity is low in extrapulmonary specimens, due to the low bacillary load in such samples. A next-generation assay has recently been developed to try to overcome this limitation. Read More

View Article
September 2018
4 Reads

Time to sputum culture conversion and its determinants among Multi-drug resistant Tuberculosis patients at public hospitals of the Amhara Regional State: A multicenter retrospective follow up study.

PLoS One 2018 21;13(6):e0199320. Epub 2018 Jun 21.

Department of Epidemiology and Biostatistics, Institute of Public Health, College of Medicine and Health Sciences, University of Gondar, Gondar, Ethiopia.

Background: In Ethiopia, Multi-drug resistant Tuberculosis (MDR-TB) is one of the major public health problems that need great attention. Time to sputum culture conversion is often used as an early predictive value for the final treatment outcome. Although guidelines for MDR-TB are frequently designed, medication freely provided, and centers for treatment duly expanded, studies on time to sputum culture conversion have been very limited in Ethiopia. Read More

View Article
June 2018
14 Reads

Chronic infection with Mycobacterium lepraemurium induces alterations in the hippocampus associated with memory loss.

Sci Rep 2018 Jun 13;8(1):9063. Epub 2018 Jun 13.

Departamento de Inmunología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Carpio y Plan de Ayala, Colonia Santo Tomás, 11340, Ciudad de México, Mexico.

Murine leprosy, caused by Mycobacterium lepraemurium (MLM), is a chronic disease that closely resembles human leprosy. Even though this disease does not directly involve the nervous system, we investigated a possible effect on working memory during this chronic infection in Balb/c mice. We evaluated alterations in the dorsal region of the hippocampus and measured peripheral levels of cytokines at 40, 80, and 120 days post-infection. Read More

View Article
June 2018
2 Reads

Development, Interlaboratory Evaluations, and Application of a Simple, High-Throughput Serum Bactericidal Assay.

mSphere 2018 06 13;3(3). Epub 2018 Jun 13.

Department of Enteric Infections, Bacterial Diseases Branch, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA

is an important cause of diarrhea worldwide, with serotypes 2a, 3a, and demonstrating epidemiological prevalence. Many development efforts are focused on lipopolysaccharide (LPS)-based vaccines, as O antigen-specific conjugate vaccines are immunogenic and efficacious. Immunization with vaccines containing LPS can elicit antibodies capable of killing in a serotype-specific manner. Read More

View Article
June 2018
8 Reads

Imaging Ca2+ Responses During Shigella Infection of Epithelial Cells.

J Vis Exp 2018 05 24(135). Epub 2018 May 24.

Equipe Communication Intercellulaire et Infections Microbiennes, Centre de Recherche Interdisciplinaire en Biologie (CIRB), Collège de France; Institut National de la Santé et de la Recherche Médicale (Inserm), U1050; Centre Nationale de la Recherche Scientifique (CNRS), UMR7241; MEMOLIFE Laboratory of Excellence and Paris Sciences et Lettres;

Ca is a ubiquitous ion involved in all known cellular processes. While global Ca responses may affect cell fate, local variations in free Ca cytosolic concentrations, linked to release from internal stores or an influx through plasma membrane channels, regulate cortical cell processes. Pathogens that adhere to or invade host cells trigger a reorganization of the actin cytoskeleton underlying the host plasma membrane, which likely affects both global and local Ca signaling. Read More

View Article
May 2018
9 Reads

Carvacrol activity & morphological changes in Mycobacterium tuberculosis.

Future Microbiol 2018 06 7;13:877-888. Epub 2018 Jun 7.

Department of Clinical Analysis & Biomedicine, State University of Maringa, Paraná, Brazil.

Aim: Evaluating carvacrol, derivatives and carvacrol plus anti-TB (anti-tuberculous) drug combination activities in Mycobacterium tuberculosis as well as carvacrol cytotoxicity, efflux pump inhibitor activity and morphological changes in M. tuberculosis HRv.

Methods: Carvacrol (CAR) and derivatives' activities were determined by resazurin microtiter assay and drug interaction by resazurin drug combination microtiter. Read More

View Article

Enteric pathogens and associated risk factors among under-five children with and without diarrhea in Wegera District, Northwestern Ethiopia.

Pan Afr Med J 2018 24;29:72. Epub 2018 Jan 24.

Faculty of Medicine, Addis Ababa University, Ethiopia.

Introduction: Childhood diarrhea is highly prevalent in slums in developing countries, but it remains understudied. The objectives of this study were to explore the prevalence of , rotavirus and bacterial enteropathogens among diarrheic and non-diarrheic children and investigate socio-environmental determinants of diarrhea in two Ethiopian towns.

Methods: A cross-sectional study was conducted from June to October 2016. Read More

View Article
June 2018
1 Read

-Specific Antibodies in Multibacillary Leprosy Patients Decrease During and After Treatment With Either the Regular 12 Doses Multidrug Therapy (MDT) or the Uniform 6 Doses MDT.

Front Immunol 2018 14;9:915. Epub 2018 May 14.

Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Goiânia, Brazil.

Leprosy serology reflects the bacillary load of patients and multidrug therapy (MDT) reduces -specific antibody titers of multibacillary (MB) patients. The () compared outcomes of regular 12 doses MDT/R-MDT and the uniform 6 doses MDT/U-MDT for MB leprosy, both of regimens including rifampicin, clofazimine, and dapsone. This study investigated the impact of R-MDT and U-MDT and the kinetic of antibody responses to specific antigens in MB patients from the . Read More

View Article
May 2018
14 Reads