2,690 results match your criteria BMC Veterinary Research [Journal]


Expression profile analysis of 5-day-old neonatal piglets infected with porcine Deltacoronavirus.

BMC Vet Res 2019 Apr 16;15(1):117. Epub 2019 Apr 16.

College of Animal Science, South China Agricultural University, Tianhe District, Wushan Road 483, Guangzhou, 510642, China.

Background: Porcine deltacoronavirus (PDCoV) is a novel coronavirus that can cause diarrhea in nursing piglets. This study was aimed to investigate the roles of host differentially expressed genes on metabolic pathways in PDCoV infections.

Results: Twenty thousand six hundred seventy-four differentially expressed mRNAs were identified in 5-day-old piglets responded to PDCoV experimental infections. Read More

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http://dx.doi.org/10.1186/s12917-019-1848-2DOI Listing

Visual detection of porcine epidemic diarrhea virus using a novel reverse transcription polymerase spiral reaction method.

BMC Vet Res 2019 Apr 15;15(1):116. Epub 2019 Apr 15.

College of Animal Science, South China Agricultural University, Guangzhou, 510642, People's Republic of China.

Background: Porcine epidemic diarrhea virus (PEDV) is a major etiological agent of porcine epidemic diarrhea around the world. Point-of-care testing in the field is lacking owing to the requirement for a simple, robust field applicable test that does not require professional laboratory equipment. The aim of this study was to establish a novel reverse transcription polymerase spiral reaction (RT-PSR) assay for the rapid detection of porcine epidemic diarrhea virus (PEDV). Read More

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http://dx.doi.org/10.1186/s12917-019-1851-7DOI Listing

Deep dermal and subcutaneous canine hemangiosarcoma in the perianal area: diagnosis of perianal mass in a dog.

Authors:
Eun Wha Choi

BMC Vet Res 2019 Apr 15;15(1):115. Epub 2019 Apr 15.

Department of Veterinary Clinical Pathology, College of Veterinary Medicine & Institute of Veterinary Science, Kangwon National University, 1 Kangwondaehak-gil, Chuncheon, Gangwon-do, 24341, Republic of Korea.

Background: Tumors of the perianal area occur frequently in dogs, and the two most common tumors are perianal gland adenoma and anal sac adenocarcinoma; others such as mast cell tumor, lymphoma and melanoma can also occur at this site. Diagnostic cytology is a useful technique and is usually used to establish a definitive diagnosis of some tumors in veterinary medicine. This report describes an extremely rare case of a deep dermal and subcutaneous canine hemangiosarcoma in the perianal area. Read More

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http://dx.doi.org/10.1186/s12917-019-1852-6DOI Listing
April 2019
1 Read

The dynamic distribution of duck Tembusu virus in the spleen of infected shelducks.

BMC Vet Res 2019 Apr 11;15(1):112. Epub 2019 Apr 11.

Ministry of Education Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Wei gang No.1, Nanjing, 210095, Jiangsu Province, China.

Background: Duck Tembusu virus (DTMUV) is a novel member of Flavivirus. The isolated and purified DTMUV strain XZ-2012 was used as a strain model, to intramuscularly inject the six-month egg-laying shelducks with the infective dose of 10TCID. The dynamic distribution of the virus in spleen at different time post-infection (pi) was studied using RT-PCR, RT-qPCR, ELISA, immunofluorescence and transmission electron microscopy (TEM). Read More

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http://dx.doi.org/10.1186/s12917-019-1860-6DOI Listing

The in vitro behaviour of canine osteoblasts derived from different bone types.

BMC Vet Res 2019 Apr 11;15(1):114. Epub 2019 Apr 11.

Comparative Biomedical Sciences, Skeletal Biology Group, The Royal Veterinary College, Royal College Street, London, NW1 0TU, UK.

Background: Our understanding of the biology of osteoblasts is important as they underpin bone remodelling, fracture healing and processes such as osseointegration. Osteoblasts isolated from human humeral samples display distinctive biological activity in vitro, which relates to the samples' bone types (subchondral (S), trabecular (T), cortical (C)). Our aim was to isolate primary osteoblast cultures from different bone types from the proximal femur of a clinical population of dogs presented for total hip replacement and compare the behaviour of the osteoblasts derived from different bone types, to identify a preferred bone type for isolation. Read More

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https://bmcvetres.biomedcentral.com/articles/10.1186/s12917-
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http://dx.doi.org/10.1186/s12917-019-1857-1DOI Listing
April 2019
2 Reads

H3N2 canine influenza virus and Enterococcus faecalis coinfection in dogs in China.

BMC Vet Res 2019 Apr 11;15(1):113. Epub 2019 Apr 11.

College of Veterinary Medicine, China Agricultural University, No.2 Yuanmingyuan West Road, Beijing, 100193, China.

Background: In May 2017, 17 dogs in a German Shepherd breeding kennel in northern China developed respiratory clinical signs. The owner treated the dogs with an intravenous injection of Shuang-Huang-lian, a traditional Chinese medicine, and azithromycin. The respiratory signs improved 3 days post-treatment, however, cysts were observed in the necks of eight dogs, and three of them died in the following 2 days. Read More

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https://bmcvetres.biomedcentral.com/articles/10.1186/s12917-
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http://dx.doi.org/10.1186/s12917-019-1832-xDOI Listing
April 2019
2 Reads

Development of a nanoparticle-assisted PCR assay for detection of bovine respiratory syncytial virus.

BMC Vet Res 2019 Apr 11;15(1):110. Epub 2019 Apr 11.

Institute of Special Animal and Plant Sciences, Chinese Academy of Agricultural Sciences, Changchun, 130112, China.

Background: Bovine respiratory syncytial virus (BRSV) is a common pathogen causing respiratory disease in cattle and a significant contributor to the bovine respiratory disease (BRD) complex. BRSV is widely distributed around the world, causing severe economic losses. This study we established a new molecular detection method of BRSV pathogen NanoPCR attributed to the combination of nano-particles in traditional PCR (Polymerase chain reaction) technology. Read More

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http://dx.doi.org/10.1186/s12917-019-1858-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6458741PMC
April 2019
1 Read

Analysis of long non-coding RNAs in neonatal piglets at different stages of porcine deltacoronavirus infection.

BMC Vet Res 2019 Apr 11;15(1):111. Epub 2019 Apr 11.

College of Animal Science, South China Agricultural University, Tianhe District, Wushan Road 483, Guangzhou, 510642, China.

Background: PDCoV (Porcine Deltacoronavirus) is a novel porcine coronavirus that causes intestinal necrosis of piglets, thinning of the intestinal wall and severe villus atrophy in the small intestine. PDCoV is a highly contagious infectious disease characterized by diarrhea, dehydration and vomiting. It has been reported that lncRNA has a significant effect on viral replication and increased or decreased virulence. Read More

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http://dx.doi.org/10.1186/s12917-019-1862-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6458635PMC

Characterization of minimal lesions related to the presence of visna/maedi virus in the mammary gland and milk of dairy sheep.

BMC Vet Res 2019 Apr 10;15(1):109. Epub 2019 Apr 10.

Veterinary Pathology Unit, School of Veterinary Medicine, University of León, León, Spain.

Background: In order to characterize the complete range of lesions, especially minimal, affecting mammary gland and viral antigen distribution and target cells using immunohistochemistry in naturally Visna/maedi (VM) 84 infected sheep were studied, forty-four from flocks with clinical cases (A) and 35 randomly sampled from two abattoirs (B) together with five negative controls (C). An immunocytochemistry technique was developed and further milk samples (n = 39) were used to study viral excretion, carrier cells and the role of milk and colostrum in the transmission of the disease.

Results: All sheep from group C and three sheep from group B were negative to VM in tissue sections by histopathology, immunohistochemistry and PCR, and also in serum using ELISA. Read More

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https://bmcvetres.biomedcentral.com/articles/10.1186/s12917-
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http://dx.doi.org/10.1186/s12917-019-1855-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6456970PMC
April 2019
2 Reads

First detection and phylogenetic analysis of porcine circovirus type 2 in raccoon dogs.

BMC Vet Res 2019 Apr 8;15(1):107. Epub 2019 Apr 8.

Hebei Key Laboratory of Preventive Veterinary Medicine, College of Animal Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao, 066004, China.

Background: Porcine circovirus type 2 (PCV2) is a major emerging virus of porcine circovirus-associated disease (PCVAD), which has brought huge economic losses to the global pig industry. Pigs are well known as the natural reservoir of PCV2. Recently, many researchers have revealed PCV2 could infect many other mammals like mice, calves, minks, dogs and goats. Read More

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http://dx.doi.org/10.1186/s12917-019-1856-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6454600PMC

Prevalence of Salmonella in broiler chickens in Kagoshima, Japan in 2009 to 2012 and the relationship between serovars changing and antimicrobial resistance.

BMC Vet Res 2019 Apr 8;15(1):108. Epub 2019 Apr 8.

Laboratory of Veterinary Public Health, Joint Faculty of Veterinary Medicine, Kagoshima University, 1-21-24 Korimoto, Kagoshima, 890-0065, Japan.

Background: This study aimed to examine the prevalence, serovars, and antimicrobial resistance of Salmonella isolates from broiler chickens in Kagoshima, Japan. A total of 192 flocks and 3071 samples were collected from broiler chickens at local farms in Kagoshima, Japan from 2009 to 2012.

Result: Among the tested farms, 49. Read More

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http://dx.doi.org/10.1186/s12917-019-1836-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6454760PMC

Pathogenic potential and the role of clones and plasmids in beta-lactamase-producing E. coli from chicken faeces in Vietnam.

BMC Vet Res 2019 Apr 4;15(1):106. Epub 2019 Apr 4.

Department of Pathology and Microbiology, Faculty of Veterinary Medicine, Université de Montréal, the Swine and Poultry Infectious Diseases Research Centre (CRIPA) and the Research Group on Zoonoses and Public Health (GREZOSP), St-Hyacinthe, Quebec, Canada.

Background: Antimicrobial resistance (AMR) in food-producing animals is a global public health issue. This study investigated AMR and virulence profiles of E. coli isolated from healthy chickens in Vietnam. Read More

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http://dx.doi.org/10.1186/s12917-019-1849-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6449924PMC

Selected prebiotics and synbiotics administered in ovo can modify innate immunity in chicken broilers.

BMC Vet Res 2019 Apr 3;15(1):105. Epub 2019 Apr 3.

Department of Animal Biotechnology and Genetics, UTP University of Science and Technology, Mazowiecka 28, 85-084, Bydgoszcz, Poland.

Background: A previous study showed that prebiotics and synbiotics administered in ovo into the egg air cell on the 12th day of incubation enhance the growth and development of chickens. However, the influence of this procedure on the development and efficiency of the innate immune system of broiler chickens is unclear. Therefore, the aim of this study was to evaluate whether the early (on the 12th day of embryo development) in ovo administration of selected prebiotics (inulin - Pre1 and Bitos - Pre2) and synbiotics (inulin + Lactococcus lactis subsp. Read More

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http://dx.doi.org/10.1186/s12917-019-1850-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6448256PMC
April 2019
1 Read

A dual colour FISH method for routine validation of sexed Bos taurus semen.

BMC Vet Res 2019 Apr 3;15(1):104. Epub 2019 Apr 3.

Institute of Molecular and Cell Biology, University of Tartu, Riia 23, 51010, Tartu, Estonia.

Background: Usage of sexed semen that allows to choose the gender of the calves, is commonly practiced in livestock industry as a profitable breeding alternative, especially in dairy farming. The flow cytometric cell sorting is the only commercially available method for bovine sperm sexing. For validation of the sexing procedure several methods have been developed including sperm fluorescence in situ hybridisation techniques. Read More

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http://dx.doi.org/10.1186/s12917-019-1839-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6446427PMC

Development and application of multiplex PCR method for simultaneous detection of seven viruses in ducks.

BMC Vet Res 2019 Apr 1;15(1):103. Epub 2019 Apr 1.

MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China.

Background: Major viruses, including duck-origin avian influenza virus, duck-origin Newcastle disease virus, novel duck parvovirus, duck hepatitis A virus, duck Tembusu virus, fowl adenovirus, and duck enteritis virus, pose great harm to ducks and cause enormous economic losses to duck industry. This study aims to establish a multiplex polymerase chain reaction (m-PCR) method for simultaneous detection of these seven viruses.

Results: Specific primers were designed and synthesized according to the conserved region of seven viral gene sequences. Read More

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https://bmcvetres.biomedcentral.com/articles/10.1186/s12917-
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http://dx.doi.org/10.1186/s12917-019-1820-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6444421PMC
April 2019
1 Read

Molecular identification and epidemiological comparison of Cryptosporidium spp. among different pig breeds in Tibet and Henan, China.

BMC Vet Res 2019 Mar 28;15(1):101. Epub 2019 Mar 28.

College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450002, People's Republic of China.

Background: Cryptosporidium spp. are important zoonotic pathogens infecting a wide range of vertebrate hosts, and causing moderate to severe diarrhea in humans. Cryptosporidium infections are frequently reported in humans and animals worldwide, but little research has been done on local pig breeds such as Tibetan pigs and Yunan Black pigs and imported pig breeds such as Landrace pigs in China. Read More

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http://dx.doi.org/10.1186/s12917-019-1847-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6438020PMC
March 2019
1 Read
1.777 Impact Factor

Polymelia in a chimeric Simmental calf: nociceptive withdrawal reflex, anaesthetic and analgesic management, anatomic and genetic analysis.

BMC Vet Res 2019 Mar 29;15(1):102. Epub 2019 Mar 29.

Department of Veterinary Clinical Science, Anaesthesiology and Pain Therapy Division, Vetsuisse Faculty, University of Bern, Länggassstrasse 124, 3012, Bern, Switzerland.

Background: Polymelia is a congenital defect characterized by one or more supernumerary legs. The genetics and aetiology of this condition in cattle have not yet been thoroughly investigated even though several case reports do exist. The model of the nociceptive withdrawal reflex (NWR) has been characterized in several species to study spinal nociceptive processing. Read More

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http://dx.doi.org/10.1186/s12917-019-1846-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6440010PMC
March 2019
1 Read

Clinical, laboratory and ultrasonographic findings in 87 cows with type-4 abomasal ulcer.

BMC Vet Res 2019 Mar 25;15(1):100. Epub 2019 Mar 25.

Department of Farm Animals, Vetsuisse-Faculty, University of Zurich, Winterthurerstrasse 260, CH-8057, Zurich, Switzerland.

Background: This study evaluated the clinical, laboratory, ultrasonographic and pathological findings in 87 cows aged 2 to 10 years (4.5 ± 1.5 years) with type-4 abomasal ulcer. Read More

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https://bmcvetres.biomedcentral.com/articles/10.1186/s12917-
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http://dx.doi.org/10.1186/s12917-019-1844-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6434897PMC
March 2019
4 Reads

In vitro ovicidal effect of a Senecio brasiliensis extract and its fractions on Haemonchus contortus.

BMC Vet Res 2019 Mar 25;15(1):99. Epub 2019 Mar 25.

Programa de Pós-Graduação em Bioexperimentação, Universidade de Passo Fundo, Passo Fundo, Rio Grande do Sul, Brasil.

Background: Haemonchosis affects sheep husbandry and its treatment is often compromised due to the development of anthelminthic resistance. Plant-derived bioactive compounds have been studied as alternative to control Haemonchus contortus. The objective of this study was to evaluate the effect of Senecio brasiliensis extracts on H. Read More

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http://dx.doi.org/10.1186/s12917-019-1843-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6434854PMC
March 2019
1 Read

Effects of Baicalin on piglet monocytes involving PKC-MAPK signaling pathways induced by Haemophilus parasuis.

BMC Vet Res 2019 Mar 25;15(1):98. Epub 2019 Mar 25.

Biochemistry and Molecular Biology, University of New Mexico School of Medicine, Albuquerque, NM, 87131, USA.

Background: Haemophilus parasuis (HPS) is the causative agent of Glässer's disease, characterized by arthritis, fibrinous polyserositis and meningitis, and resulting in worldwide economic losses in the swine industry. Baicalin (BA), a commonly used traditional Chinese medication, has been shown to possess a series of activities, such as anti-bacterial, anti-viral, anti-tumor, anti-oxidant and anti-inflammatory activities. However, whether BA has anti-apoptotic effects following HPS infection is unclear. Read More

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http://dx.doi.org/10.1186/s12917-019-1840-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6434632PMC
March 2019
2 Reads

Identification of very early inflammatory markers in a porcine myocardial infarction model.

BMC Vet Res 2019 Mar 12;15(1):91. Epub 2019 Mar 12.

Stem Cell Therapy Unit, Jesús Usón Minimally Invasive Surgery Centre, 10071, Cáceres, Spain.

Background: Acute myocardial infarction (AMI) is one of the most deleterious conditions leading to cardiovascular diseases and mortality. The importance of an early and accurate diagnosis assures immediate medical treatments, which are fundamental to reduce mortality and improve prognoses. AMI is associated to an inflammatory response which includes the increase of circulating inflammatory cytokines, chemokines and immune cell activation. Read More

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http://dx.doi.org/10.1186/s12917-019-1837-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6427889PMC
March 2019
1 Read

Rapid and visual detection of Lawsonia intracellularis with an improved recombinase polymerase amplification assay combined with a lateral flow dipstick.

BMC Vet Res 2019 Mar 21;15(1):97. Epub 2019 Mar 21.

College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan, China.

Background: Lawsonia intracellularis (L. intracellularis) is the etiologic agent of porcine proliferative enteropathy (PPE), which is reported in many swine breeding countries all over the world, and has caused enormous economic losses in intensive pig production systems. Therefore, the aim of this study was to develop a simple and rapid method for on-site detection of Lawsonia intracellularis (L. Read More

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http://dx.doi.org/10.1186/s12917-019-1841-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6429820PMC
March 2019
1 Read

Indication of metabolic inflexibility to food intake in spontaneously overweight Labrador Retriever dogs.

BMC Vet Res 2019 Mar 20;15(1):96. Epub 2019 Mar 20.

Department of Anatomy, Physiology and Biochemistry, Faculty of Veterinary Medicine and Animal Science, Swedish University of Agricultural Sciences, Box 7011, 75007, Uppsala, Sweden.

Background: Obesity in dogs is an increasing problem associated with morbidity, shortened life span and poor life quality. Overweight dogs exhibit postprandial hyperlipidaemia, highlighting the need to identify potential dysregulations in lipid metabolism. This study investigated metabolites related to lipid metabolism (i. Read More

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http://dx.doi.org/10.1186/s12917-019-1845-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6425671PMC
March 2019
1 Read

Seroprevalence of antibodies for bovine viral diarrhoea virus, Brucella abortus and Neospora caninum, and their roles in the incidence of abortion/foetal loss in dairy cattle herds in Nakuru District, Kenya.

BMC Vet Res 2019 Mar 18;15(1):95. Epub 2019 Mar 18.

Department of Health Management, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, Canada.

Background: No comprehensive studies have been carried out on the infectious causes of abortion in Kenyan dairy cattle herds. A survey was carried out to determine the seroprevalence of antibodies against Bovine Viral Diarrhoea Virus (BVDV), Brucella abortus (BA) and Neospora caninum (NC) among dairy cattle herds in Nakuru County, a major dairying area in Kenya. A prospective sero-epidemiological study was also undertaken to investigate the effects of BVDV, BA and NC on the occurrence of bovine abortion in dairy cattle herds, where monthly rectal palpations for pregnancy were performed, and monthly serum samples were tested for antibodies to the 3 pathogens. Read More

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http://dx.doi.org/10.1186/s12917-019-1842-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6423779PMC
March 2019
3 Reads

Antimicrobial use for selected diseases in cats in Switzerland.

BMC Vet Res 2019 Mar 14;15(1):94. Epub 2019 Mar 14.

Clinic for Small Animal Internal Medicine, Vetsuisse Faculty, University of Zurich, Winterthurerstrasse 260, CH-8057, Zurich, Switzerland.

Background: Antibiotic use in human and veterinary medicine is considered a main driver of antimicrobial resistance. Although guidelines to promote appropriate use of antimicrobials in veterinary patients have been developed, antibiotic overprescription is assumed to be a common problem. The goal of this study was to investigate antimicrobial use in cats in Switzerland with acute upper respiratory tract disease (aURTD), feline lower urinary tract disease (FLUTD) and abscesses, and to assess compliance of prescription with consensus guidelines. Read More

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http://dx.doi.org/10.1186/s12917-019-1821-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6417182PMC
March 2019
2 Reads

High prevalence of hepatitis E virus infection among domestic pigs in Ibaraki Prefecture, Japan.

BMC Vet Res 2019 Mar 12;15(1):87. Epub 2019 Mar 12.

Department of Virology II, National Institute of Infectious Diseases, Musashimurayama, Tokyo, Japan.

Background: Hepatitis E virus (HEV) is prevalent in pigs and may serve as a reservoir for human infection. However, data on HEV infections in pigs in Ibaraki Prefecture, Japan, are limited. Here, we clarified the process and course of HEV in naturally infected pigs. Read More

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http://dx.doi.org/10.1186/s12917-019-1816-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6416891PMC
March 2019
6 Reads

Long-term computed tomography follow-up in great Danes with or without signs of osseous- associated cervical Spondylomyelopathy.

BMC Vet Res 2019 Mar 12;15(1):90. Epub 2019 Mar 12.

College of Veterinary Medicine, The Ohio State University, 601 Vernon L. Tharp St, Columbus, OH, USA.

Background: Osseous- associated cervical spondylomyelopathy (OA-CSM) has a high prevalence in Great Danes. In order to understand the progression of osseous changes, we aimed to perform a long-term computed tomographic (CT) follow-up study of Great Dane dogs with and without OA-CSM. Canine CSM is comparable to a common neurologic disease often diagnosed in older people termed cervical spondylotic myelopathy or degenerative cervical myelopathy, which is progressive in nature. Read More

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http://dx.doi.org/10.1186/s12917-019-1835-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6416867PMC
March 2019
2 Reads

A European interlaboratory trial to evaluate the performance of different PCR methods for Mycoplasma bovis diagnosis.

BMC Vet Res 2019 Mar 12;15(1):86. Epub 2019 Mar 12.

Anses, Lyon Laboratory, JRU Ruminants Mycoplasmoses, Anses, VetAgro Sup, University of Lyon, Lyon, France.

Background: Several species-specific PCR assays, based on a variety of target genes are currently used in the diagnosis of Mycoplasma bovis infections in cattle herds with respiratory diseases and/or mastitis. With this diversity of methods, and the development of new methods and formats, regular performance comparisons are required to ascertain diagnostic quality. The present study compares PCR methods that are currently used in six national veterinary institutes across Europe. Read More

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http://dx.doi.org/10.1186/s12917-019-1819-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6417145PMC
March 2019
1 Read

A detection of benzimidazole resistance-associated SNPs in the isotype-1 β-tubulin gene in Haemonchus contortus from wild blue sheep (Pseudois nayaur) sympatric with sheep in Helan Mountains, China.

BMC Vet Res 2019 Mar 12;15(1):89. Epub 2019 Mar 12.

College of Wildlife Resources, Northeast Forestry University, Harbin, China.

Background: Benzimidazole (BZ) resistance is an increasingly serious problem due to the excessive use of this anthelmintic for controlling Haemonchus contortus, which is one of the major gastrointestinal nematodes infecting small ruminants worldwide. Three known single nucleotide polymorphisms (SNPs), F167Y (TAC), E198A (GCA) and F200Y (TAC), in the isotype-1 β-tubulin gene of H. contortus are associated with BZ resistance. Read More

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http://dx.doi.org/10.1186/s12917-019-1838-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6416841PMC
March 2019
1 Read

Congenital intestinal stenosis and Hirschsprung's disease: two extremely rare pathologies in a newborn puppy.

BMC Vet Res 2019 Mar 13;15(1):92. Epub 2019 Mar 13.

Department of Reproductive Biology, National Institute of Medical Sciences and Nutrition Salvador Zubirán, Avenue. Vasco de Quiroga 15 Col. Belisario Domínguez, Section XVI, Tlalpan, 14080, México City, Mexico.

Background: Hirschsprung's disease (HSCR) is a common congenital malformation of the enteric nervous system (ENS). During fetal development, ganglion cells of the ENS are derived from neural crest cells that migrate to the bowel. These cells reside principally in two ganglionated plexus: 1) The myenteric plexus, extending from the esophagus to the anus, and 2) submucous plexus, extending from the duodenum to the anus. Read More

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http://dx.doi.org/10.1186/s12917-019-1806-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6416937PMC
March 2019
2 Reads

Identification and antimicrobial susceptibility profile of Escherichia coli isolated from backyard chicken in and around ambo, Central Ethiopia.

BMC Vet Res 2019 Mar 11;15(1):85. Epub 2019 Mar 11.

School of Veterinary Medicine, Wolaita Sodo University, P O Box 138, Sodo, Ethiopia.

Background: Escherichia coli is bacteria that exist as commensal in the intestine of animals and humans, but pathogenic strains cause disease in chickens. The development of antimicrobial resistance in E. coli is one of major concern worldwide. Read More

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http://dx.doi.org/10.1186/s12917-019-1830-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6417126PMC
March 2019
1 Read

White blood cell count in birds: evaluation of a commercially available method.

BMC Vet Res 2019 Mar 14;15(1):93. Epub 2019 Mar 14.

Clinical Laboratory, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland.

Background: To conduct a hematological analysis of avian blood samples, standard automated cell counting is unreliable because all avian blood cells are nucleated. Therefore, quantitative white blood cell counting in birds is still performed manually, whereby the Natt-Herrick method is widely used in veterinary laboratories. The aim of this study was to evaluate a new commercially available single test system for avian white blood cell counting, the Natt-Herricks-Tic®, which would allow easy in-house analysis by clinicians or technicians. Read More

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http://dx.doi.org/10.1186/s12917-019-1834-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6417236PMC
March 2019
1 Read

Identification and genomic analysis of two novel duck-origin GPV-related parvovirus in China.

BMC Vet Res 2019 Mar 12;15(1):88. Epub 2019 Mar 12.

Guangdong Haid Institute of Animal Husbandry & Veterinary, Guangzhou, 511400, China.

Background: Since early 2015, mule duck and Cherry Valley duck flocks have been suffering from short beak and dwarfism syndrome. This widely spreading infectious disease is characterized by growth retardation, smaller beak and tarsus with high morbidity and low mortality rate. For better understanding, we identified and characterized virus isolates named AH and GD from diseased Cherry Valley duck and mule duck flocks and investigated the damage caused by novel parvovirus-related virus (NGPV) to tissues and organs, including kidney, brain, pancreas, liver, spleen, bursa of fabricius and myocardial tissues. Read More

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https://bmcvetres.biomedcentral.com/articles/10.1186/s12917-
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http://dx.doi.org/10.1186/s12917-019-1833-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6417286PMC
March 2019
15 Reads

Molecular detection and phylogenetic analysis of Peste des petits ruminants virus circulating in small ruminants in eastern Amhara region, Ethiopia.

BMC Vet Res 2019 Mar 8;15(1):84. Epub 2019 Mar 8.

Department of clinical studies, College of Veterinary Medicine and Agriculture, Addis Ababa University, Bishoftu, Ethiopia.

Background: Peste des Petits Ruminants (PPR) is a severe, highly infectious and fatal viral disease of small ruminants. Four lineages of PPR virus have been identified globally based on sequence analysis of the nucleoprotein (N) and fusion (F) gene. The aim of this study was to isolate and genetically characterize recently circulating PPR virus in small ruminants in the eastern Amhara region in Ethiopia. Read More

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http://dx.doi.org/10.1186/s12917-019-1828-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6408798PMC
March 2019
1 Read

Basal and dynamics mRNA expression of muscular HSP108, HSP90, HSF-1 and HSF-2 in thermally manipulated broilers during embryogenesis.

BMC Vet Res 2019 Mar 8;15(1):83. Epub 2019 Mar 8.

Department of Physiology, Biochemistry and Pharmacology, College of Veterinary Medicine, King Faisal University, P.O. Box 400, Al-Hufof, 31982, Saudi Arabia.

Background: Limited data are available about the kinetics of mRNA expressions of Heat shock proteins (HSPs) and heat shock factors (HSFs) in the thermally manipulated (TM) broiler chicks during acute heat stress. Therefore, this study aimed to investigate effects of thermal manipulation (TM) of broiler chicken during embryonic days (ED) 12-18 on the basal and dynamics mRNA expression of heat shock proteins (HSP108 and HSP90) and heat shock factors (HSF-1 and HSF-2) in the muscle tissue during late embryogenesis, first week of life and during heat stress (HS) on post-hatch days 14 and 28. One thousand and five hundred fertile Ross 315 broiler eggs were randomly allocated to five groups: control group (37. Read More

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http://dx.doi.org/10.1186/s12917-019-1827-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6408791PMC
March 2019
3 Reads

Coordinated expression of vascular endothelial growth factor A and urokinase-type plasminogen activator contributes to classical swine fever virus Shimen infection in macrophages.

BMC Vet Res 2019 Mar 8;15(1):82. Epub 2019 Mar 8.

School of Life Science and Technology, Xidian University, Xi'an, Shaanxi, 710071, People's Republic of China.

Background: The Shimen strain of classical swine fever (CSF) virus (CSFV) causes CSF, which is mainly characterised by disseminated intravascular haemorrhage. Macrophages are an essential component of innate immunity against pathogenic microorganisms; however, the role of macrophages in CSF pathogenesis remains unclear. To illuminate the infective mechanism of CSFV, we used gene co-expression networks derived from macrophages infected with CSFV Shimen and CSFV C as well as uninfected macrophages to screen key regulatory genes, and their contributions to the pathogenesis of CSF were discussed. Read More

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http://dx.doi.org/10.1186/s12917-019-1826-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6407193PMC
March 2019
2 Reads

Risk factors for new bovine brucellosis infections in Colombian herds.

BMC Vet Res 2019 Mar 7;15(1):81. Epub 2019 Mar 7.

Animal Medicine and Health Department, Faculty of Veterinary Medicine, Campus of The Autonomous University of Barcelona (UAB), 08193 Bellaterra, Barcelona, Spain.

Background: Bovine brucellosis is a zoonotic disease that causes substantial economic losses and has a strong impact on public health. The main objective of this paper is to determine the risk factors for new infections of Brucella abortus on Colombian cattle farms previously certified as being free of brucellosis. A case-control study was conducted by comparing 98 cases (farms certified as brucellosis-free for three or more years but became infected) with 93 controls (farms that remained brucellosis-free during at least the previous three years). Read More

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https://bmcvetres.biomedcentral.com/articles/10.1186/s12917-
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http://dx.doi.org/10.1186/s12917-019-1825-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6404332PMC
March 2019
9 Reads

Humoral immune response to repeated lumpy skin disease virus vaccination and performance of serological tests.

BMC Vet Res 2019 Mar 6;15(1):80. Epub 2019 Mar 6.

Friedrich-Loeffler-Institut, Südufer 10, D-17493, Greifswald-Insel Riems, Germany.

Background: In the presented study we investigated the development of the humoral immune response against LSDV during the process of re-vaccination of cattle over a time span of 5 months. In addition, the performance of different serological techniques for antibody detection against LSDV was compared. For sample collection, an area without previous LSD outbreak reports in Serbia was selected. Read More

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http://dx.doi.org/10.1186/s12917-019-1831-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6404298PMC
March 2019
2 Reads

Feasibility of a disposable canister-free negative-pressure wound therapy (NPWT) device for treating open wounds in horses.

BMC Vet Res 2019 Mar 6;15(1):78. Epub 2019 Mar 6.

Département de biomédecine vétérinaire, Université de Montréal, 3200 rue Sicotte, St-Hyacinthe, QC, J2S 2M2, Canada.

Background: Wounds are among the most common medical conditions affecting horses and have a major economic impact on the horse industry. Wound healing in horses is distinct to that documented in other species, and often results in delayed healing and extensive scarring, with compromised functional and aesthetic outcomes. To date, there is no conventional method objectively proven to accelerate healing or to successfully prevent complications associated with second intention healing. Read More

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http://dx.doi.org/10.1186/s12917-019-1829-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6404353PMC
March 2019
1 Read

Effect of green tea and mulberry leaf powders on the gut microbiota of chicken.

BMC Vet Res 2019 Mar 6;15(1):77. Epub 2019 Mar 6.

School of Life Science, Huizhou University, Huizhou, 516007, China.

Background: The gut microbiota is closely correlated with host health and is strongly influenced by food composition. Chinese herbs are usually used as natural feed additives in livestock production. Therefore, the present study assessed the influence of diet supplementation with green tea and mulberry leaf powders on the chicken gut microbiota. Read More

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https://bmcvetres.biomedcentral.com/articles/10.1186/s12917-
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http://dx.doi.org/10.1186/s12917-019-1822-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6404333PMC
March 2019
7 Reads

Development and application of a baculovirus-expressed capsid protein-based indirect ELISA for detection of porcine circovirus 3 IgG antibodies.

BMC Vet Res 2019 Mar 6;15(1):79. Epub 2019 Mar 6.

Hunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines, Laboratory of Functional Proteomics, Research Center of Reverse Vaccinology, College of Veterinary Medicine, Hunan Agricultural University, Changsha, 410128, China.

Background: Porcine circovirus type 3 (PCV3), recently widely isolated from pigs with various clinical conditions, is likely globally epidemic. However, development of serological diagnosis for PCV3 in pigs is ongoing. Our objectives were to: 1) establish an indirect ELISA, using PCV3 capsid protein (Cap) prepared by Baculovirus Expression Vector System (BEVS) as a high-quality coating antigen for detection of PCV3-associated antibodies in serum samples; and 2) use this ELISA to conduct a serological survey for PCV3 in various regions of Hunan province, China. Read More

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http://dx.doi.org/10.1186/s12917-019-1810-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6404275PMC
March 2019
1 Read
1.777 Impact Factor

Ultrasonography-guided removal of plant-based foreign bodies from the lacrimal sac in four dogs.

BMC Vet Res 2019 Mar 5;15(1):76. Epub 2019 Mar 5.

Department of Veterinary Science, University of Pisa, via livornese snc, San Piero a Grado, 56122, Pisa, Italy.

Background: Dacryocystitis is an inflammation of the lacrimal drainage system. One of the most common causes of dacryocystitis in dogs is due to plant-based foreign bodies typically located in the lacrimal sac. The aim of this case series was to describe an ultrasonography-guided technique for dogs with plant-based foreign bodies in the lacrimal sac, as both a diagnostic and therapeutic tool. Read More

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http://dx.doi.org/10.1186/s12917-019-1817-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6402165PMC
March 2019
4 Reads

A spatial assessment of Nipah virus transmission in Thailand pig farms using multi-criteria decision analysis.

BMC Vet Res 2019 Mar 4;15(1):73. Epub 2019 Mar 4.

Spatial epidemiology Lab. (SpELL), University of Brussels, Brussels, Belgium.

Background: Thailand's Central Plain is identified as a contact zone between pigs and flying foxes, representing a potential zoonotic risk. Nipah virus (NiV) has been reported in flying foxes in Thailand, but it has never been found in pigs or humans. An assessment of the suitability of NiV transmission at the spatial and farm level would be useful for disease surveillance and prevention. Read More

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http://dx.doi.org/10.1186/s12917-019-1815-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6399983PMC
March 2019
3 Reads

Raw meat-based diets for dogs: survey of owners' motivations, attitudes and practices.

BMC Vet Res 2019 Mar 4;15(1):74. Epub 2019 Mar 4.

Department of Animal Medicine, Production and Health, University of Padua, Viale dell'Università 16, 35020, Legnaro, PD, Italy.

Background: The popularity of raw meat-based diets (RMBDs) for pets has been increasing in recent years even if the reputed health benefits are mainly anecdotal. A web-based survey was developed to better understand the motivations and habits of owners who decided to feed their dogs RMBDs.

Results: The questionnaire was completed by 218 dog owners, 62 of whom were living with people whose immune system was impaired or weakened. Read More

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http://dx.doi.org/10.1186/s12917-019-1824-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6399943PMC
March 2019
4 Reads

Immune related genes as markers for monitoring health status of honey bee colonies.

BMC Vet Res 2019 Mar 4;15(1):72. Epub 2019 Mar 4.

VISAVET Centre and Animal Health Department, Veterinary School, Complutense University of Madrid, Madrid, Spain.

Background: Honey bee population decline threatens the beekeeping sector, agriculture and global biodiversity. Early detection of colony mortality may facilitate rapid interventions to contain and prevent mortality spread. Among others, deformed wing virus (DWV) is capable of inducing colony losses, especially when combined with Varroa destructor mite. Read More

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http://dx.doi.org/10.1186/s12917-019-1823-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6398266PMC
March 2019
5 Reads

Effects of divergent selection upon adrenocortical activity on immune traits in pig.

BMC Vet Res 2019 Mar 4;15(1):71. Epub 2019 Mar 4.

IECM, INRA, Oniris, La Chantrerie, CS 40706, F-44307, Nantes Cedex 3, France.

Background: The sustainability of farming and animal welfare requires the reconsideration of current selection schemes. In particular, implementation of new selection criteria related to animal health and welfare should help to produce more robust animals and to reduce anti-microbial use. The hypothalamo-pituitary-adrenocortical (HPA) axis plays a major role in metabolic regulation and adaptation processes and its activity is strongly influenced by genetic factors. Read More

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http://dx.doi.org/10.1186/s12917-019-1809-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6398250PMC
March 2019
6 Reads

Primary angle-closure glaucoma with goniodysgenesis in a Beagle dog.

BMC Vet Res 2019 Mar 4;15(1):75. Epub 2019 Mar 4.

Department of Small Animal Clinical Sciences, Veterinary Medical Center, College of Veterinary Medicine, Michigan State University, 736 Wilson Road, East Lansing, MI, 48824, USA.

Background: Open angle glaucoma is the only type of primary glaucoma reported in Beagles. This case report describes a primary angle-closure glaucoma in a Beagle and its diagnostic and prognostic relevance.

Case Presentation: A 12-year-old, neutered male Beagle presented to the Michigan State University (MSU) Comparative Ophthalmology Service for evaluation of suspected visual impairment. Read More

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http://dx.doi.org/10.1186/s12917-019-1812-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6399873PMC
March 2019
4 Reads

Specificity of serological test for detection of tuberculosis in cattle, goats, sheep and pigs under different epidemiological situations.

BMC Vet Res 2019 Mar 1;15(1):70. Epub 2019 Mar 1.

Unidad de Inmunología Microbiana, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Madrid, Spain.

Background: Serum antibody detection has potential as a complementary diagnostic tool in animal tuberculosis (TB) control, particularly in multi-host systems. The objective of the present study was to assess the specificity (Sp) of an enzyme-linked immunosorbent assay (ELISA) based on the new multiprotein complex P22 for the detection of specific antibodies against the Mycobacterium tuberculosis complex (MTC) in the four most relevant domestic animals acting as MTC hosts: cattle, goat, sheep and pig. We used sera from an officially TB-free (OTF) country, Norway, and from a non-OTF one, Spain. Read More

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http://dx.doi.org/10.1186/s12917-019-1814-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6397464PMC
March 2019
2 Reads

Field validation of clinical and laboratory diagnosis of wildebeest associated malignant catarrhal fever in cattle.

BMC Vet Res 2019 Feb 28;15(1):69. Epub 2019 Feb 28.

International Livestock Research Institute, Old Naivasha Road, P. O. Box 30709, Nairobi, Kenya.

Background: Wildebeest associated malignant catarrhal fever (WA-MCF) is a fatal disease of cattle. Outbreaks are seasonal and associated with close interaction between cattle and calving wildebeest. In Kenya, WA-MCF has a dramatic effect on cattle-keepers who lose up to 10% of their cattle herds per year. Read More

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http://dx.doi.org/10.1186/s12917-019-1818-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6396541PMC
February 2019
4 Reads

Effects of bovine tumor necrosis factor alpha decoy receptors on cell death and inflammatory cytokine kinetics: potential for bovine inflammation therapy.

BMC Vet Res 2019 Feb 28;15(1):68. Epub 2019 Feb 28.

Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido, 060-0818, Japan.

Background: Refractory diseases, including bacterial infections, are causing huge economic losses in dairy farming. Despite efforts to prevent and treat those diseases in cattle, including the use of antimicrobials, it is not well controlled in the field. Several inflammatory cytokines, including tumor necrosis factor alpha (TNF-α), play important roles in disease progression; thus, blocking these cytokines can attenuate the acute and sever inflammation and may be a novel strategy for treatment. Read More

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https://bmcvetres.biomedcentral.com/articles/10.1186/s12917-
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http://dx.doi.org/10.1186/s12917-019-1813-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6396486PMC
February 2019
6 Reads