6,069 results match your criteria BMC Research Notes [Journal]

Brief report: Parental attitudes and knowledge on routine childhood immunization: an experience from Central Sri Lanka.

BMC Res Notes 2018 Jun 22;11(1):402. Epub 2018 Jun 22.

Department of Microbiology, Faculty of Medicine, University of Peradeniya, Peradeniya, Sri Lanka.

Objectives: A lack of correct awareness about immunization among parents put them at risk of falling prey to the anti-vaccine movement. This risk is present even in countries with a high vaccine uptake. This study was done with the objective of assessing the awareness of parents childhood vaccination. Read More

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Integration of conventional cell viability assays for reliable and reproducible read-outs: experimental evidence.

BMC Res Notes 2018 Jun 22;11(1):403. Epub 2018 Jun 22.

DAILAB, DBT-AIST International Center for Translational and Environmental Research (DAICENTER), National Institute of Advanced Industrial Science & Technology (AIST), 1-1-1 Higashi, Tsukuba, 305-8565, Japan.

Objective: Short-term viability assays of cultured cells in 96-well plates are routinely used to determine the cytotoxicity or safety of drugs. These are often based on the formation of chromogen, generated selectively in viable cells. The innate problems of such short-term cell viability assays include (i) effect of drugs is determined by cell density (ii) some drugs have slow/gradual effect and hence may escape such assays, (iii) cell morphology that reveal significant hints to molecular signaling underlining the effect of drugs cannot be effectively captured, (iv) long-term effect on viability and clonogenic potential of cells cannot be determined and (v) herbal extracts often possess intrinsic color that interferes with spectrophotometer estimation. Read More

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Investigating the functionality of a ribosome-binding mutant of NAA15 using Saccharomyces cerevisiae.

BMC Res Notes 2018 Jun 22;11(1):404. Epub 2018 Jun 22.

Department of Biological Sciences, University of Bergen, 5006, Bergen, Norway.

Objective: N-terminal acetylation is a common protein modification that occurs preferentially co-translationally as the substrate N-terminus is emerging from the ribosome. The major N-terminal acetyltransferase complex A (NatA) is estimated to N-terminally acetylate more than 40% of the human proteome. To form a functional NatA complex the catalytic subunit NAA10 must bind the auxiliary subunit NAA15, which properly folds NAA10 for correct substrate acetylation as well as anchors the entire complex to the ribosome. Read More

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Tumor analysis: freeze-thawing cycle of triple-negative breast cancer cells alters tumor CD24/CD44 profiles and the percentage of tumor-infiltrating immune cells.

BMC Res Notes 2018 Jun 20;11(1):401. Epub 2018 Jun 20.

COSS (Chemistry Oncogenesis Stress Signaling)-UMR 1242, Inserm, Univ Rennes, CLCC Eugène Marquis, Rue Bataille Flandres Dunkerque, 35042, Rennes, France.

Objective: The use of novel methods to characterize living tumor cells relies on well-conceived biobanks. Herein, we raised the question of whether the composition of fresh and freeze/thawed dissociated tumor samples is comparable in terms of quantitative and qualitative profiling.

Results: Breast cancer is a heterogeneous disease, encompassing luminal A and B, basal/triple-negative breast cancer (TNBC), and ERBB2-like tumors. Read More

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Value of peripheral blood count for dengue severity prediction.

BMC Res Notes 2018 Jun 20;11(1):400. Epub 2018 Jun 20.

Department of Medicine, Faculty of Medicine, University of Peradeniya, Peradeniya, Sri Lanka.

Objective: This retrospective study was conducted in 2017 with the objective of evaluating the value of acute phase peripheral blood parameters in predicting dengue haemorrhagic fever (DHF). Patients, who were admitted to Teaching Hospital Peradeniya between January and August 2017 due to dengue illness, were recruited into this study.

Results: A total of 515 patients participated in the study. Read More

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The efficacy of high-dose penicillin G for pneumococcal pneumonia diagnosed based on initial comprehensive assessment at admission: an observational study.

BMC Res Notes 2018 Jun 20;11(1):399. Epub 2018 Jun 20.

Department of Internal Medicine, National Hospital Organization Tochigi Medical Center, 1-10-37, Nakatomatsuri, Utsunomiya, Tochigi, 3208580, Japan.

Objectives: High-dose penicillin therapy is effective in approximately 90% of pneumococcal pneumonia cases diagnosed based on urinary pneumococcal antigen tests or Gram staining at admission. The efficacy of high-dose penicillin therapy for pneumococcal pneumonia diagnosed based on an initial comprehensive assessment comprising a syndromic approach, Gram staining of sputum and urinary pneumococcal antigen testing was investigated.

Results: Seventy adult patients diagnosed with pneumococcal pneumonia based on an initial comprehensive assessment and treated with high-dose penicillin G at admission were included. Read More

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The remarkable plethora of infestation-responsive Q-type C2H2 transcription factors in potato.

BMC Res Notes 2018 Jun 19;11(1):398. Epub 2018 Jun 19.

Invasive Insect Biocontrol and Behavior Lab, USDA-ARS, 10300 Baltimore Ave., BARC-West Bldg 007, Rm 301, Beltsville, MD, 20705, USA.

Objective: Q-type C2H2 transcription factors (TF) play crucial roles in the plant response to stress, often leading to regulation of downstream genes required for tolerance to these challenges. An infestation-responsive Q-type C2H2 TF (StZFP2) is induced by wounding and infestation in potato. While mining the Solanum tuberosum Group Phureja genome for additional members of this family of proteins, five StZFP2-like genes were found on a portion of chromosome 11. Read More

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Mitochondrial genome variation of Atlantic cod.

BMC Res Notes 2018 Jun 19;11(1):397. Epub 2018 Jun 19.

Genomics Group, Faculty of Biosciences and Aquaculture, Nord University, 8049, Bodø, Norway.

Objective: The objective of this study was to analyse intraspecific sequence variation of Atlantic cod mitochondrial DNA, based on a comprehensive collection of completely sequenced mitochondrial genomes.

Results: We determined the complete mitochondrial DNA sequence of 124 cod specimens from the eastern and western part of the species' distribution range in the North Atlantic Ocean. All specimens harboured a unique mitochondrial DNA haplotype. Read More

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Case report: Diffuse hyperplastic perilobar nephroblastomatosis complicated by a unilateral Wilms tumour: diagnosis, treatment and follow-up.

BMC Res Notes 2018 Jun 19;11(1):396. Epub 2018 Jun 19.

Department of Radiology, The University of Calgary, 3330 Hospital Dr NW, Calgary, AB, T2N 4N1, Canada.

Background: Nephroblastomatosis is an uncommon pathologic process characterized by the presence of persistent embryonic nephrogenic rests. Progression to Wilms tumour occurs in an estimated 35% of patients. Cure rates are based on histologic findings and disease stage and have improved from 10% in the 1920s to over 90% today. Read More

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Confirmation of covalently-linked structure and cell-death inducing activity in site-specific chemical conjugates of human Fas ligand extracellular domain.

BMC Res Notes 2018 Jun 15;11(1):395. Epub 2018 Jun 15.

Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8566, Japan.

Objective: In this study, we aimed to identify the structural components and to clarify the biological activity in the site-specific conjugates of human Fas ligand extracellular domain (hFasLECD) with either fluorescein moiety (FL) or chicken egg-white avidin (Avi). The conjugates were characterized by molecular-weight measurement using MALDI-TOF mass-spectrometric analysis and by cell-death inducing activity measurement against a human colorectal cancer cell line, HT-29, using MTT cell-viability assay. Pretreatment effect with human interferon-γ (IFN-γ) on the cell-death inducing activity was evaluated. Read More

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Acceptability and feasibility of magnetic femoral nerve stimulation in older, functionally impaired patients.

BMC Res Notes 2018 Jun 15;11(1):394. Epub 2018 Jun 15.

Division of Molecular and Clinical Medicine, Ninewells Hospital, University of Dundee, Dundee, Scotland, UK.

Objective: Magnetic femoral nerve stimulation to test muscle function has been largely unexplored in older people. We assessed acceptability, feasibility, along with reproducibility and correlation with other physical function measures.

Results: Study 1 recruited older people with sarcopenia. Read More

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Self-assessment differences between genders in a low-stakes objective structured clinical examination (OSCE).

BMC Res Notes 2018 Jun 15;11(1):393. Epub 2018 Jun 15.

Division of Hematology, Department of Medicine, Faculty of Medicine, University of Ottawa, Ottawa, Canada.

Objective: Physicians and medical students are generally poor-self assessors. Research suggests that this inaccuracy in self-assessment differs by gender among medical students whereby females underestimate their performance compared to their male counterparts. However, whether this gender difference in self-assessment is observable in low-stakes scenarios remains unclear. Read More

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Topolnogical classifier for detecting the emergence of epileptic seizures.

BMC Res Notes 2018 Jun 14;11(1):392. Epub 2018 Jun 14.

Computer Science Division, School of Science and Technology, University of Camerino, Via Madonna delle Carceri, 9, 62032, Camerino, IT, Italy.

Objective: An innovative method based on topological data analysis is introduced for classifying EEG recordings of patients affected by epilepsy. We construct a topological space from a collection of EEGs signals using Persistent Homology; then, we analyse the space by Persistent entropy, a global topological feature, in order to classify healthy and epileptic signals.

Results: The performance of the resulting one-feature-based linear topological classifier is tested by analysing the Physionet dataset. Read More

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Hereditary hearing loss SNP-microarray pilot study.

BMC Res Notes 2018 Jun 14;11(1):391. Epub 2018 Jun 14.

Institute of Human Genetics, Julius Maximilians University, Würzburg, Germany.

Objectives: Despite recent advancements in diagnostic tools, the genomic landscape of hereditary hearing loss remains largely uncharacterized. One strategy to understand genome-wide aberrations includes the analysis of copy number variation that can be mapped using SNP-microarray technology. A growing collection of literature has begun to uncover the importance of copy number variation in hereditary hearing loss. Read More

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An enChIP system for the analysis of bacterial genome functions.

BMC Res Notes 2018 Jun 14;11(1):387. Epub 2018 Jun 14.

Department of Biochemistry and Genome Biology, Hirosaki University Graduate School of Medicine, 5 Zaifu-cho, Hirosaki, Aomori, 036-8562, Japan.

Objective: The engineered DNA-binding molecule-mediated chromatin immunoprecipitation (enChIP) technology enables purification of specific genomic regions interacting with their associated molecules. In enChIP, the locus to be purified is first tagged with engineered DNA-binding molecules. An example of such engineered DNA-binding molecules to tag the locus of interest is the clustered regularly interspaced short palindromic repeats (CRISPR) system, consisting of a catalytically-inactive form of Cas9 (dCas9) and guide RNA (gRNA). Read More

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Mammalian expression vectors for metabolic biotinylation tandem affinity tagging by co-expression in cis of a mammalian codon-optimized BirA biotin ligase.

BMC Res Notes 2018 Jun 14;11(1):390. Epub 2018 Jun 14.

Institute of Molecular Biology and Biotechnology, Foundation of Research & Technology Hellas, 100 Nikolaou Plastira Street, 70013, Heraklion, Crete, Greece.

ΟBJECTIVE: To construct mammalian expression vectors for the N- or C-terminal tagging of proteins with a tandem affinity tag comprised of the biotinylatable Avi tag and of a triple FLAG tag.

Results: We constructed and tested by transient transfections mammalian expression vectors for the co-expression from a single plasmid of N- or C-terminally tagged proteins bearing a tandem affinity tag comprised of the biotinylatable Avi tag and of a triple FLAG tag separated by a tobacco etch virus (TEV) protease cleavage site, together with a mammalian codon-optimized BirA biotin ligase fused to green fluorescent protein. We also describe platform vectors for the N- or C-terminal AVI-TEV-FLAG tagging of any complementary DNA of choice. Read More

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Liver transplant score for prediction of biliary atresia patients' survival following Kasai procedure.

BMC Res Notes 2018 Jun 13;11(1):381. Epub 2018 Jun 13.

Pediatric Surgery Division, Department of Surgery, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada/Dr. Sardjito Hospital, Jl. Kesehatan No. 1, Yogyakarta, 55281, Indonesia.

Objectives: Recently, a scoring system has been developed to predict which patients with biliary atresia (BA) who underwent a Kasai procedure should be considered for liver transplant. Here, we applied the scoring system to predict the survival of BA patients following the Kasai procedure at Dr. Sardjito Hospital, Yogyakarta, Indonesia from January 2012 to January 2016. Read More

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June 2018
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Knowledge, attitudes and skills in melanoma diagnosis among doctors: a cross sectional study from Sri Lanka.

BMC Res Notes 2018 Jun 14;11(1):389. Epub 2018 Jun 14.

National Hospital of Sri Lanka, Colombo, 01000, Sri Lanka.

Objectives: This study aimed to assess the knowledge, attitudes and skills of non-specialist doctors on timely referral of suspicious lesions for melanoma diagnosis.

Results: One hundred and twenty-three doctors (mean age; 30.4 years, SD ± 8. Read More

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June 2018
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Identification of a new allele of the Dw gene causing brachytic dwarfing in peach.

BMC Res Notes 2018 Jun 14;11(1):386. Epub 2018 Jun 14.

IRTA, Centre de Recerca en Agrigenòmica CSIC-IRTA-UAB-UB, Campus UAB, Cerdanyola del Vallès (Bellaterra), 08193, Barcelona, Spain.

Objective: Peach brachytic dwarfism determined by Dwarf gene (Dw) is an undesired trait segregating in some peach breeding programs. Recently, a single nucleotide polymorphism (SNP) mutation in the gibberellin insensitive dwarf 1 (GID1) peach gene causing brachytic dwarfism was described. In this research we wanted to validate this marker in an F population of the 'Nectavantop' peach cultivar (Nv) to include it as a marker assisted selection tool for peach breeding programs. Read More

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The impact of bariatric surgery on the resolution of obstructive sleep apnoea.

BMC Res Notes 2018 Jun 14;11(1):385. Epub 2018 Jun 14.

Department of Upper Gastrointestinal and Bariatric Surgery, Worcestershire Acute Hospitals NHS Trust, Worcester, WR5 1DD, UK.

Objective: Obesity is associated with a high incidence of obstructive sleep apnoea (OSA). Bariatric surgery is postulated to lead to OSA resolution, but there is inconclusive evidence on its efficacy. We used objective measurements to determine the rate of resolution or improvement of OSA in patients who had bariatric procedures in our unit. Read More

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The association between the lipid profile and fasting blood glucose with weight related outcomes in healthy obese adults.

BMC Res Notes 2018 Jun 14;11(1):383. Epub 2018 Jun 14.

Patanjali Research Foundation, Patanjali Yogpeeth, Haridwar, Uttarakhand, 249405, India.

Objectives: The present study was conducted on healthy obese persons to determine: (i) the association between total cholesterol, triglycerides, HDL cholesterol, total cholesterol/HDL ratio and fasting blood glucose (FBG) with (a) BMI, (b) waist circumference (WC) and (c) body fat and (ii) the presence of undiagnosed type 2 diabetes (based on fasting blood glucose) in the participants. There were 1140 participants of both sexes (female:male 697:443; group mean age 44.0 ± 10. Read More

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Detection of Ehrlichia ruminantium infection in cattle in Cameroon.

BMC Res Notes 2018 Jun 14;11(1):388. Epub 2018 Jun 14.

Laboratory for Emerging Infectious Diseases, University of Buea, P. O. Box 63, Buea, Cameroon.

Objectives: Ehrlichia ruminantium infection (heartwater) is a major constraint that impacts negatively on the cattle industry development in sub-Saharan Africa and so far, little is known of the presence of heartwater in cattle in Cameroon. This study sought to investigate the prevalence of E. ruminantium infection in cattle in Cameroon and to determine the predictors of infection. Read More

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Evaluation of buccal swabs for pharmacogenetics.

BMC Res Notes 2018 Jun 14;11(1):382. Epub 2018 Jun 14.

GenXys Health Care Systems Inc., Vancouver, BC, Canada.

Objective: A simple, non-invasive sample collection method is key for the integration of pharmacogenetics into clinical practice. The aim of this study was to gain samples for pharmacogenetic testing and evaluate the variation between dry-flocked and sponge-tipped buccal swabs in yield and quality of DNA isolated.

Results: Thirty-one participants collected samples using dry-flocked swabs and sponge-tipped swabs. Read More

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A cost effective RFLP method to genotype Solute carrier organic anion 1B1 (SLCO1B1) c.1929A>C (p.Leu643Phe, rs34671512); a variant with potential effect on rosuvastatin pharmacokinetics.

BMC Res Notes 2018 Jun 14;11(1):384. Epub 2018 Jun 14.

Division of Human Genetics, Department of Pathology, Faculty of Health Sciences, Anzio Road, Observatory, Cape Town, South Africa.

Objective: This study describes a restriction fragment polymorphism protocol for rapidly screening the polymorphism SLCO1B1 c.1929A>C in genomic DNA samples. The polymorphism SLCO1B1 c. Read More

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June 2018
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Prevention of haematoma progression by tranexamic acid in intracerebral haemorrhage patients with and without spot sign on admission scan: a statistical analysis plan of a pre-specified sub-study of the TICH-2 trial.

BMC Res Notes 2018 Jun 13;11(1):379. Epub 2018 Jun 13.

Department of Neurology, Bispebjerg Hospital, Copenhagen University Hospital, Bispebjerg bakke 23, 2400, Copenhagen, Denmark.

Objective: We present the statistical analysis plan of a prespecified Tranexamic Acid for Hyperacute Primary Intracerebral Haemorrhage (TICH)-2 sub-study aiming to investigate, if tranexamic acid has a different effect in intracerebral haemorrhage patients with the spot sign on admission compared to spot sign negative patients. The TICH-2 trial recruited above 2000 participants with intracerebral haemorrhage arriving in hospital within 8 h after symptom onset. They were included irrespective of radiological signs of on-going haematoma expansion. Read More

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Role of temperature in reported chickenpox cases in northern European countries: Denmark and Finland.

Ayako Sumi

BMC Res Notes 2018 Jun 13;11(1):377. Epub 2018 Jun 13.

Department of Hygiene, Sapporo Medical University School of Medicine, S-1, W-17, Chuo-ku, Sapporo, Hokkaido, 060-8556, Japan.

Objective: In this study, we sought to explore the temperature-dependent transition of patterns of reported chickenpox cases in the northern European countries of Denmark and Finland to help determine the potential relationship with epidemiological factors of the disease. We performed time-series analysis consisting of a spectral analysis based on the maximum entropy method in the frequency domain and the nonlinear least squares method in the time domain, using the following time-series data: monthly data of reported chickenpox cases and mean temperatures in the pre-vaccination era for Denmark and Finland. The results were compared with those reported for China and Japan in our previous studies. Read More

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Genetic screen identifies a requirement for SMN in mRNA localisation within the Drosophila oocyte.

BMC Res Notes 2018 Jun 13;11(1):378. Epub 2018 Jun 13.

Department of Physiology and Biochemistry, Faculty of Medicine and Surgery, University of Malta, Msida, Malta.

Objective: Spinal muscular atrophy (SMA) results from insufficient levels of the survival motor neuron (SMN) protein. Drosophila is conducive to large-scale genetic-modifier screens which can reveal novel pathways underpinning the disease mechanism. We tested the ability of a large collection of genomic deletions to enhance SMN-dependent lethality. Read More

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Magnitude of diabetes self-care practice and associated factors among type two adult diabetic patients following at public Hospitals in central zone, Tigray Region, Ethiopia, 2017.

BMC Res Notes 2018 Jun 13;11(1):380. Epub 2018 Jun 13.

Department of Nursing and Midwifery, School of Allied Health Science, College of Health Science, Addis Ababa University, Addis Ababa, Ethiopia.

Objective: Self-care practice in type two diabetes is a critical factor to keep the disease under control. Despite the important role of self-care practices in management of diabetes were recognized to be useful and effective in achieving diabetes control and preventing its complication, findings of previous studies in Ethiopia were confirmed that, aspects of self-care practices were more problematic. So that this study focus on magnitude of self-care practice and associated factors among diabetic patients. Read More

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Correction to: Spontaneous rhythms in a harbor seal pup calls.

Andrea Ravignani

BMC Res Notes 2018 Jun 11;11(1):376. Epub 2018 Jun 11.

Research Department, Sealcentre Pieterburen, Hoofdstraat 94a, 9968 AG, Pieterburen, The Netherlands.

Following publication of the original article [1], the author reported an error in the first sentence of the 'Sound recordings' section. The recordings mentioned in this sentence were performed 6 days later than written. In this Correction article the original and corrected version of the sentence are presented. Read More

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Self-rated health, quality of life and appetite as predictors of initiation of dialysis and mortality in patients with chronic kidney disease stages 4-5: a prospective cohort study.

BMC Res Notes 2018 Jun 8;11(1):371. Epub 2018 Jun 8.

Department of Renal Medicine, Aarhus University Hospital, Palle Juul-Jensens Boulevard 99, 8200, Aarhus N, Denmark.

Objective: Patient-reported health status, including symptom burden, functional status and quality of life, are important measures of health in patients with chronic kidney disease. We aimed to investigate patient-reported outcomes (PRO) on self-rated health, appetite, quality of life and their associations with clinical outcomes. We conducted a prospective observational cohort study. Read More

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Analysis of tuberculosis treatment outcomes among pulmonary tuberculosis patients in Bahawalpur, Pakistan.

BMC Res Notes 2018 Jun 8;11(1):370. Epub 2018 Jun 8.

Massey University, Auckland, New Zealand.

Objective: Monitoring tuberculosis treatment outcomes and understanding the reasons for unsuccessful treatment are important indicators for evaluating the performance of the national tuberculosis control program. The aim of this study was to evaluate the treatment outcomes among pulmonary TB (PTB) patients and identify the predictors of unsuccessful treatment outcome.

Results: Treatment success rate of 67. Read More

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Comparison of TPLO tibial tuberosity fractures with and without an in situ rotational pin.

BMC Res Notes 2018 Jun 8;11(1):368. Epub 2018 Jun 8.

Department of Surgery Affiliated Veterinary Specialists, 9905 S US Hwy 17-92, Maitland, FL, 32751, USA.

Objective: Cranial cruciate ligament (CCL) rupture is a common cause of pelvic limb lameness in dogs. The tibial plateau leveling osteotomy (TPLO) is a well-described surgical procedure that treats CCL ruptures. The objective of this study was to compare the risk of tibial tuberosity fractures from TPLO procedures using a TPLO reduction pin in situ versus patients with a TPLO reduction pin removed at the time of surgery. Read More

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Trend of hypertension morbidity and mortality in Tigray Region from 2011 to 2015, Tigray, Ethiopia.

BMC Res Notes 2018 Jun 8;11(1):375. Epub 2018 Jun 8.

Department of Biomedical Sciences, College of Health Sciences & Referral Hospital, Aksum University, Axum, Ethiopia.

Objective: Hypertension in developing countries were not considered as a major concern. This lack of concern can be explained by the health policy and research of the past years, which had its main focus on infectious diseases but this study focuses on 4 years trend of hypertension mortality and morbidity. A facility based retrospective health management information system record assessment was carried out. Read More

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Effect of the 5-HT receptor agonist tegaserod on the expression of GRK2 and GRK6 in the rat gastrointestinal tract.

BMC Res Notes 2018 Jun 8;11(1):362. Epub 2018 Jun 8.

Monash Institute of Pharmaceutical Sciences, Monash University (Parkville Campus), 381 Royal Parade, Parkville, VIC, 3052, Australia.

Objective: Tegaserod is a 5-hydroxytryptamine type 4 (5-HT) receptor agonist, formerly used in treating constipation predominant irritable bowel syndrome, which desensitizes 5-HT receptors in rat oesophagus and colon in vitro. Desensitization of 5-HT receptors is regulated by G-protein coupled receptor kinases. This study was designed to assess the effect of 5-HT receptor activation on the expression of GRK2 and GRK6 in the rat oesophagus and distal colon by acute administration of tegaserod. Read More

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Sucroferric oxyhydroxide decreases serum phosphorus level and fibroblast growth factor 23 and improves renal anemia in hemodialysis patients.

BMC Res Notes 2018 Jun 8;11(1):363. Epub 2018 Jun 8.

Department of Kidney Disease, Kawashima Hospital, 1-39 Kitasakoichiban-cho, Tokushima, 770-0011, Japan.

Objective: Sucroferric oxyhydroxide, a novel iron-based phosphate-binder, has been shown to have beneficial effects in lowering serum phosphorus levels and improving renal anemia in clinical studies. Although an effect of this agent on fibroblast growth factor 23 (FGF23) has been reported in an animal study, there is little clinical data supporting this finding. This study aimed to evaluate the effect on chronic kidney disease-mineral and bone disorder, FGF23, renal anemia, iron-related parameters, adverse events of sucroferric oxyhydroxide in hemodialysis patients. Read More

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Handgrip exercise by the non-affected hand increases venous return in the contralateral axillary vein in patients with stroke: a pilot study.

BMC Res Notes 2018 Jun 8;11(1):374. Epub 2018 Jun 8.

Faculty of Care and Rehabilitation, Seijoh University, 2-172 Fukinodai, Tokai, Aichi, 476-8588, Japan.

Objective: Treatment of hand edema is important for maintaining upper limb function in patients with stroke, although the effects of many such treatments have been limited. This study aimed to examine, using ultrasound, the effect of handgrip exercise by the non-affected hand of stroke patients on venous return in the affected upper limb.

Results: Seven men participated, within 6 months of a unilateral first-ever stroke. Read More

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Cytokeratin 5/6 and cytokeratin 8/18 expression in triple negative breast cancers: clinicopathologic significance in South-Asian population.

BMC Res Notes 2018 Jun 8;11(1):372. Epub 2018 Jun 8.

Brown University, Providence, RI, USA.

Objective: Cytokeratin 5/6 and Cytokeratin 8/18 are basal and luminal markers of breast cancer and they have pathological and prognostic significance in breast cancer. We performed Cytokeratin 5/6 and CK8/18 immunohistochemistry on 150 cases of triple negative breast cancers and association with various clinicopathological features was evaluated.

Results: Positive CK5/6 expression was noted in 8% (12 cases) of TNBC while 2. Read More

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Prevalence of resistance to integrase strand-transfer inhibitors (INSTIs) among untreated HIV-1 infected patients in Morocco.

BMC Res Notes 2018 Jun 8;11(1):369. Epub 2018 Jun 8.

Faculty of Medicine and Pharmacy, University Mohammed V in Rabat, Av. Mohamed Belarbi El Alaoui, 6203, Rabat, Morocco.

Objective: The integrase strand-transfer inhibitors (INSTIs) are an important class in the arsenal of antiretroviral drugs designed to block the integration of HIV-1 cDNA into the host DNA through the inhibition of DNA strand transfer. In this study for the first time in Morocco, the complete HIV-1 integrase gene was analysed from newly diagnosed patients to evaluate the prevalence of natural polymorphisms and INSTIs resistance-associated mutations in the integrase gene.

Results: The 864pb IN coding region was successfully sequenced from plasma sample for 77 among 80 antiretroviral naïve patients. Read More

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Antibody titers in turkeys increase after multiple booster vaccinations with an attenuated Salmonella live vaccine.

BMC Res Notes 2018 Jun 8;11(1):367. Epub 2018 Jun 8.

Clinic for Poultry, University of Veterinary Medicine Hannover, Bünteweg 17, Hannover, Germany.

Objective: Human Salmonellosis is one of the most frequently reported foodborne zoonoses in the European Union. The most common source of human infections is the consumption of poultry products. Besides management and hygiene practices vaccination of poultry livestock is seen as one way to reduce Salmonella infections in humans. Read More

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June 2018
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Assessment of glycemic control in type 2 diabetes in the Eastern Sudan.

BMC Res Notes 2018 Jun 8;11(1):373. Epub 2018 Jun 8.

Faculty of Medicine, University of Khartoum, P. O. Box 102, Khartoum, Sudan.

Objectives: A cross-sectional study was conducted in Gadarif, eastern Sudan to assess glycaemic control among adult patients with type 2 diabetes in eastern Sudan. Poor glycaemic control was defined as HbA1c level of ≥ 7.0%. Read More

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Male size, not female preferences influence female reproductive success in a poeciliid fish (Poecilia latipinna): a combined behavioural/genetic approach.

BMC Res Notes 2018 Jun 8;11(1):364. Epub 2018 Jun 8.

Department of Zoology, University of Oklahoma, 730 Van Vleet Oval, Norman, OK, 73019, USA.

Objective: We investigated the potential role of indirect benefits for female mate preferences in a highly promiscuous species of live-bearing fishes, the sailfin molly Poecilia latipinna using an integrative approach that combines methods from animal behavior, life-history evolution, and genetics. Males of this species solely contribute sperm for reproduction, and consequently females do not receive any direct benefits. Despite this, females typically show clear mate preferences. Read More

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Training, executive, attention and motor skills (TEAMS) training versus standard treatment for preschool children with attention deficit hyperactivity disorder: a randomised clinical trial.

BMC Res Notes 2018 Jun 8;11(1):366. Epub 2018 Jun 8.

Psychiatric Research Unit, Region Zealand Psychiatry, Slagelse, Denmark.

Objective: This study compared the effectiveness of manualised training, executive, attention, and motor skills (TEAMS) training versus standard treatment in preschool children with attention deficit hyperactivity disorder (ADHD). We conducted a randomised parallel group, single-blinded, superiority trial. The primary outcome was ADHD symptoms and the secondary outcome was functionality. Read More

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The association of latent toxoplasmosis and level of serum testosterone in humans.

BMC Res Notes 2018 Jun 8;11(1):365. Epub 2018 Jun 8.

Department of Medical Parasitology and Mycology, Tehran University of Medical Sciences, Pour Sina St., Ghods St., Enghelab St., Tehran, 1417613191, Iran.

Objectives: Latent toxoplasmosis modifies various hormones and behaviors in infected hosts and possibly involves in etiology of different neurologic and psychiatric disorders. The aim of the current study was to assess possible associations between latent toxoplasmosis and testosterone concentration in Toxoplasma infected and free subjects. Briefly, 18-49 year-old participated in the study. Read More

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Challenges to achieving low palatal fistula rates following primary cleft palate repair: experience of an institution in Uganda.

BMC Res Notes 2018 Jun 7;11(1):358. Epub 2018 Jun 7.

Mbarara University of Science and Technology, P.O.Box 1410, Mbarara, Uganda.

Objective: To determine frequency of palatal fistula following primary cleft palate repair and the associated factors as a measure of cleft palate repair outcome and its challenges at a cleft centre in Uganda.

Results: Between May and December 2016, 54 children with cleft palate were followed up at Comprehensive Rehabilitation services of Uganda (CoRSU) hospital, from time of primary cleft palate repair until at least 3 months postoperative to determine whether they developed palatal fistula or not. Frequency of palatal fistula was 35%. Read More

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Urinary exosomal expression of activator of G protein signaling 3 in polycystic kidney disease.

BMC Res Notes 2018 Jun 7;11(1):359. Epub 2018 Jun 7.

Department of Pharmaceutical Sciences, University of Tennessee Health Science Center College of Pharmacy, 881 Madison Ave, Rm 442, Memphis, TN, 38163, USA.

Objective: PKD is a genetic disease that is characterized by abnormally proliferative epithelial cells in the kidney and liver. Urinary exosomes have been previously examined as a source of unique proteins that may be used to diagnose and monitor the progression of PKD. Previous studies by our group have shown that AGS3, which is a receptor-independent regulator G-proteins, was markedly upregulated in RTECs during kidney injury including PKD. Read More

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Multiplex PCR for the simultaneous detection of the Enterobacterial gene wecA, the Shiga Toxin genes (stx and stx) and the Intimin gene (eae).

BMC Res Notes 2018 Jun 7;11(1):360. Epub 2018 Jun 7.

Department of Biosciences, University of Oslo, Box 1031, Blindern, 0316, Oslo, Norway.

Objectives: The aetiology of several human diarrhoeas has been increasingly associated with the presence of virulence factors rather than with the bacterial species hosting the virulence genes, exemplified by the sporadic emergence of new bacterial hosts. Two important virulence factors are the Shiga toxin (Stx) and the E. coli outer membrane protein (Eae) or intimin, encoded by the stx and eae genes, respectively. Read More

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Elimination of Mycoplasma contamination in Chlamydia stocks as a result of in vivo passage or plaque isolation.

BMC Res Notes 2018 Jun 7;11(1):361. Epub 2018 Jun 7.

Microbiology and Immunology Department, Chicago College of Osteopathic Medicine, Midwestern University, SH-323H, 555 31st Street, Downers Grove, IL, USA.

Objective: This study aims to eliminate Mycoplasma spp. contamination from laboratory stocks of Chlamydia spp. by in vivo passage or by plaque assay. Read More

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June 2018
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Sublingual nitroglycerin for early blood pressure control in hypertensive emergencies: observations from an emergency department clinical audit in Sri Lanka.

BMC Res Notes 2018 Jun 5;11(1):355. Epub 2018 Jun 5.

Department of Clinical Medicine, University of Colombo, Colombo, Sri Lanka.

Objective: Hypertensive emergencies are potentially life threatening and require prompt blood pressure control with intravenous agents. Preparation of intravenous infusions is time consuming. Usefulness of sublingual nitroglycerin in this setting is not known. Read More

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June 2018
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Duration taken for the anal sphincter pressures to stabilize prior to anorectal manometry.

BMC Res Notes 2018 Jun 5;11(1):354. Epub 2018 Jun 5.

Department of Surgery, Faculty of Medicine, University of Colombo, P.O. Box 271, Kynsey Road, Colombo 8, Sri Lanka.

Objectives: Anorectal manometry (ARM) is an integral part of evaluating the anal sphincter function. The current recommendation of waiting for 5 min (lead-in-time) prior to beginning the recording has no evidence. A prolonged procedure may reduce patient compliance. Read More

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June 2018
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Evaluation of virtual patient cases for teaching diagnostic and management skills in internal medicine: a mixed methods study.

BMC Res Notes 2018 Jun 5;11(1):357. Epub 2018 Jun 5.

Department of General Internal Medicine, University Health Network - Toronto General Hospital, University of Toronto, EN 14-218, 200 Elizabeth Street, Toronto, ON, M5G 2C4, Canada.

Objective: The virtual patient (VP) is a computer program that simulates real-life clinical scenarios and allows learners to make diagnostic and therapeutic decisions in a safe environment. Although many VP cases are available, few focus on junior trainees as their target audience. In addition, there is wide variability in trainees' clinical rotation experiences, based on local practice and referral patterns, duty hour restrictions, and competing educational requirements. Read More

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June 2018
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