3,613 results match your criteria BMC Microbiology[Journal]


Analysis of xanthyletin and secondary metabolites from Pseudomonas stutzeri ST1302 and Klebsiella pneumoniae ST2501 against Pythium insidiosum.

BMC Microbiol 2019 Apr 16;19(1):78. Epub 2019 Apr 16.

Department of Clinical Microbiology, Faculty of Associated Medical Sciences, Centre for Research and Development of Medical Diagnosis Laboratories, Khon Kaen University, Khon Kaen, 40002, Thailand.

Background: Pythium insidiosum is a member of the oomycetes class of aquatic fungus-like microorganisms. It can infect humans and animals through skin wounds and the eyes, causing pythiosis, an infectious disease with high morbidity and mortality rates. Antifungal agents are ineffective as pythiosis treatments because ergosterol, the target site of most antifungal agents, is not found in the P. Read More

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http://dx.doi.org/10.1186/s12866-019-1452-4DOI Listing

Phosphate uptake by the phosphonate transport system PhnCDE.

BMC Microbiol 2019 Apr 16;19(1):79. Epub 2019 Apr 16.

Departamento de Microbiologia, Instituto de Ciências Biomédicas Universidade de São Paulo, São Paulo-SP, Brazil.

Background: Phosphate is a fundamental nutrient for all creatures. It is thus not surprising that a single bacterium carries different transport systems for this molecule, each usually operating under different environmental conditions. The phosphonate transport system of E. Read More

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http://dx.doi.org/10.1186/s12866-019-1445-3DOI Listing

Occurrence and characterisation of biofilms in drinking water systems of broiler houses.

BMC Microbiol 2019 Apr 15;19(1):77. Epub 2019 Apr 15.

Flanders Research Institute for Agriculture, Fisheries and Food (ILVO), Technology and Food Science Unit, Brusselsesteenweg 370, 9090, Melle, Belgium.

Background: Water quality in the drinking water system (DWS) plays an important role in the general health and performance of broiler chickens. Conditions in the DWS of broilers are ideal for microbial biofilm formation. Since pathogens might reside within these biofilms, they serve as potential source of waterborne transmission of pathogens to livestock and humans. Read More

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http://dx.doi.org/10.1186/s12866-019-1451-5DOI Listing

gyrA and parC mutations in fluoroquinolone-resistant Neisseria gonorrhoeae isolates from Kenya.

BMC Microbiol 2019 Apr 8;19(1):76. Epub 2019 Apr 8.

US Army Medical Research Directorate-Africa, P.O Box 606, 00621, Village Market, Nairobi, Kenya.

Background: Phenotypic fluoroquinolone resistance was first reported in Western Kenya in 2009 and later in Coastal Kenya and Nairobi. Until recently gonococcal fluoroquinolone resistance mechanisms in Kenya had not been elucidated. The aim of this paper is to analyze mutations in both gyrA and parC responsible for elevated fluoroquinolone Minimum Inhibitory Concentrations (MICs) in Neisseria gonorrhoeae (GC) isolated from heterosexual individuals from different locations in Kenya between 2013 and 2017. Read More

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http://dx.doi.org/10.1186/s12866-019-1439-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6454682PMC
April 2019
2.729 Impact Factor

Next-generation sequencing for hypothesis-free genomic detection of invasive tropical infections in poly-microbially contaminated, formalin-fixed, paraffin-embedded tissue samples - a proof-of-principle assessment.

BMC Microbiol 2019 Apr 8;19(1):75. Epub 2019 Apr 8.

Institute for Microbiology, Virology and Hygiene, University Medicine Rostock, Schillingallee 70, 18057, Rostock, Germany.

Background: The potential of next-generation sequencing (NGS) for hypothesis-free pathogen diagnosis from (poly-)microbially contaminated, formalin-fixed, paraffin embedded tissue samples from patients with invasive fungal infections and amebiasis was investigated. Samples from patients with chromoblastomycosis (n = 3), coccidioidomycosis (n = 2), histoplasmosis (n = 4), histoplasmosis or cryptococcosis with poor histological discriminability (n = 1), mucormycosis (n = 2), mycetoma (n = 3), rhinosporidiosis (n = 2), and invasive Entamoeba histolytica infections (n = 6) were analyzed by NGS (each one Illumina v3 run per sample). To discriminate contamination from putative infections in NGS analysis, mean and standard deviation of the number of specific sequence fragments (paired reads) were determined and compared in all samples examined for the pathogens in question. Read More

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http://dx.doi.org/10.1186/s12866-019-1448-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6454699PMC
April 2019
1 Read

A 16S rDNA PCR-based theoretical to actual delta approach on culturable mock communities revealed severe losses of diversity information.

BMC Microbiol 2019 Apr 8;19(1):74. Epub 2019 Apr 8.

Department of Biological Sciences (DCB), State University of Santa Cruz (UESC), Pav. Jorge Amado, Rod. BR 415, Km 16, Salobrinho, Ilhéus, BA, 45662-000, Brazil.

Background: Subunits of ribosomal RNA genes (rDNAs) characterized by PCR-based protocols have been the proxy for studies in microbial taxonomy, phylogenetics, evolution and ecology. However, relevant factors have shown to interfere in the experimental outputs in a variety of systems. In this work, a 'theoretical' to 'actual' delta approach was applied to data on culturable mock bacterial communities (MBCs) to study the levels of losses in operational taxonomic units (OTUs) detectability. Read More

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http://dx.doi.org/10.1186/s12866-019-1446-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6454784PMC

Low concentrations of acetic and formic acids enhance the inactivation of Staphylococcus aureus and Pseudomonas aeruginosa with pulsed electric fields.

BMC Microbiol 2019 Apr 3;19(1):73. Epub 2019 Apr 3.

Faculty of Electronics, Vilnius Gediminas Technical University, Naugarduko st. 41, 03227, Vilnius, Lithuania.

Background: Skin infections, particularly caused by drug-resistant pathogens, represent a clinical challenge due to being a frequent cause of morbidity and mortality. The objectives of this study were to examine if low concentrations of acetic and formic acids can increase sensitivity of Staphylococcus aureus and Pseudomonas aeruginosa to pulsed electric field (PEF) and thus, promote a fast and efficient treatment methodology for wound treatment.

Results: We have shown that the combination of PEF (10-30 kV/cm) with organic acids (0. Read More

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http://dx.doi.org/10.1186/s12866-019-1447-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6448289PMC
April 2019
1 Read

Knockout of alanine racemase gene attenuates the pathogenicity of Aeromonas hydrophila.

BMC Microbiol 2019 Apr 2;19(1):72. Epub 2019 Apr 2.

College of Life Science, Hebei Normal University, Shijiazhuang, 050024, China.

Background: Aeromonas hydrophila is an opportunistic pathogen of poikilothermic and homoeothermic animals, including humans. In the present study, we described the role of Alanine racemase (alr-2) in the virulence of A. hydrophila using an alr-2 knockout mutant (A. Read More

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http://dx.doi.org/10.1186/s12866-019-1437-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6444436PMC

Efficacy of bacteriophage treatment against carbapenem-resistant Acinetobacter baumannii in Galleria mellonella larvae and a mouse model of acute pneumonia.

BMC Microbiol 2019 Apr 2;19(1):70. Epub 2019 Apr 2.

Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Y, Seoul, Republic of Korea.

Background: Acinetobacter baumannii is an opportunistic pathogen that causes serious nosocomial infection in intensive care units. In particular, carbapenem-resistant A. baumannii (CRAB) strains have been increasing in the past decade, and they have caused major medical problems worldwide. Read More

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http://dx.doi.org/10.1186/s12866-019-1443-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6444642PMC
April 2019
2 Reads

Lipopeptide mediated biocontrol activity of endophytic Bacillus subtilis against fungal phytopathogens.

BMC Microbiol 2019 Apr 2;19(1):71. Epub 2019 Apr 2.

Department of Agricultural Biotechnology, Assam Agricultural University, 785013, Jorhat, India.

Background: The use of chemical fungicides against fungal pathogens adversely affects soil and plant health thereby resulting in overall environmental hazards. Therefore, biological source for obtaining antifungal agents is considered as an environment-friendly alternative for controlling fungal pathogens.

Results: In this study, seven endophytic bacteria were isolated from sugarcane leaves and screened for its antifungal activity against 10 fungal isolates belonging to the genera Alternaria, Cochliobolus, Curvularia, Fusarium, Neodeightonia, Phomopsis and Saccharicola isolated from diseased leaves of sugarcane. Read More

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http://dx.doi.org/10.1186/s12866-019-1440-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6444643PMC
April 2019
1 Read

Proteome profiling of Pseudomonas aeruginosa PAO1 identifies novel responders to copper stress.

BMC Microbiol 2019 Apr 1;19(1):69. Epub 2019 Apr 1.

Department of Molecular Sciences, Macquarie University, Sydney, 2109, Australia.

Background: The opportunistic pathogen, Pseudomonas aeruginosa is well known for its environmental and metabolic versatility, yet many of the functions of its gene-products remain to be fully elucidated. This study's objective was to illuminate the potential functions of under-described gene-products during the medically relevant copper-stress condition.

Results: We used data-independent acquisition mass spectrometry to quantitate protein expression changes associated with copper stress in P. Read More

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http://dx.doi.org/10.1186/s12866-019-1441-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6444534PMC

Induction of the immunoprotective coat of Yersinia pestis at body temperature is mediated by the Caf1R transcription factor.

BMC Microbiol 2019 Mar 29;19(1):68. Epub 2019 Mar 29.

Institute for Cell and Molecular Biosciences, Medical School, Newcastle University, Newcastle upon Tyne, UK.

Background: Thermal regulation of gene expression occurs in many microorganisms, and is mediated via several typical mechanisms. Yersinia pestis is the causative agent of the plague and spreads by zoonotic transfer from fleas to mammalian blood with a concomitant rapid temperature change, from ambient to 37 °C, which induces the expression of capsular antigen (Caf1) that inhibits phagocytosis. Caf1 is formed into long polymeric fimbriae by a periplasmic chaperone (Caf1M) and outer membrane usher (Caf1A). Read More

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https://bmcmicrobiol.biomedcentral.com/articles/10.1186/s128
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http://dx.doi.org/10.1186/s12866-019-1444-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6440114PMC
March 2019
5 Reads

Development of flow cytometry based adherence assay for Neisseria gonorrhoeae using 5'-carboxyfluorosceinsuccidyl ester.

BMC Microbiol 2019 Mar 25;19(1):67. Epub 2019 Mar 25.

Vaccine and Infectious Disease Organization - International Vaccine Centre (VIDO-InterVac), University of Saskatchewan, Saskatoon, Saskatchewan, S7N 5E3, Canada.

Background: Neisseria gonorrhoeae is an obligate human pathogen and its adherence to host cells is essential for its pathogenesis. Gonococcal adherence assays are based on the enumeration of bacteria attached to human cells on solid media. Because conventional adherence assays are based on bacterial counts, they are often time consuming to perform and prone to observer bias. Read More

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http://dx.doi.org/10.1186/s12866-019-1438-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6434840PMC
March 2019
1 Read

Genome-wide discovery of structured noncoding RNAs in bacteria.

BMC Microbiol 2019 Mar 22;19(1):66. Epub 2019 Mar 22.

Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, USA.

Background: Structured noncoding RNAs (ncRNAs) play essential roles in many biological processes such as gene regulation, signaling, RNA processing, and protein synthesis. Among the most common groups of ncRNAs in bacteria are riboswitches. These cis-regulatory, metabolite-binding RNAs are present in many species where they regulate various metabolic and signaling pathways. Read More

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http://dx.doi.org/10.1186/s12866-019-1433-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6429828PMC

Isothermal microcalorimetry for thermal viable count of microorganisms in pure cultures and stabilized formulations.

BMC Microbiol 2019 Mar 21;19(1):65. Epub 2019 Mar 21.

Department of Molecular Sciences, Swedish University of Agricultural Sciences, P.O. Box 7015, SE-75007, Uppsala, Sweden.

Background: Quantification of viable microorganisms is an important step in microbiological research as well as in microbial product formulation to develop biological control products or probiotics. Often, the efficiency of the resulting product is dependent on the microbial cell density and their viability, which may decrease over time. Commonly, the number of viable cells is determined by serial dilution and plating techniques or flow cytometry. Read More

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http://dx.doi.org/10.1186/s12866-019-1432-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6429831PMC

Lactobacillus curvatus UFV-NPAC1 and other lactic acid bacteria isolated from calabresa, a fermented meat product, present high bacteriocinogenic activity against Listeria monocytogenes.

BMC Microbiol 2019 Mar 20;19(1):63. Epub 2019 Mar 20.

Departamento de Veterinária, Universidade Federal de Viçosa, Campus UFV, Viçosa, MG, 36570-900, Brazil.

Background: Bacteriocins produced by lactic acid bacteria (LAB) can be considered as viable alternatives for food safety and quality, once these peptides present antimicrobial activity against foodborne pathogens and spoilage bacteria. Fermented foods, such as artisanal sausages and cured meats, are relevant sources of LAB strains capable of producing novel bacteriocins, with particular interest by the food industry.

Results: Three LAB strains (firstly named as Lactobacillus curvatus 12, L. Read More

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http://dx.doi.org/10.1186/s12866-019-1436-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6425648PMC

A genomic analysis of Mycobacterium immunogenum strain CD11_6 and its potential role in the activation of T cells against Mycobacterium tuberculosis.

BMC Microbiol 2019 Mar 20;19(1):64. Epub 2019 Mar 20.

Microbial Type Culture Collection and Gene bank (MTCC), CSIR- Institute of Microbial Technology, Chandigarh, India.

Background: Mycobacterium tuberculosis (Mtb) is an etiological agent of tuberculosis (TB). Tuberculosis is a mounting problem worldwide. The only available vaccine BCG protects the childhood but not adulthood form of TB. Read More

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http://dx.doi.org/10.1186/s12866-019-1421-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6425668PMC
March 2019
2 Reads
2.729 Impact Factor

Studies of antimicrobial resistance in rare mycobacteria from a nosocomial environment.

BMC Microbiol 2019 Mar 19;19(1):62. Epub 2019 Mar 19.

Center for Neuroscience and Cell Biology (CNC), University of Coimbra, 3004-504, Coimbra, Portugal.

Background: Nontuberculous mycobacteria (NTM) are ubiquitous in nature and recognized agents of opportunistic infection, which is often aggravated by their intrinsic resistance to antimicrobials, poorly defined therapeutic strategies and by the lack of new drugs. However, evaluation of their prevalence in anthropogenic environments and the associated antimicrobial resistance profiles have been neglected. In this work, we sought to determine minimal inhibitory concentrations of 25 antimicrobials against 5 NTM isolates recovered from a tertiary-care hospital surfaces. Read More

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http://dx.doi.org/10.1186/s12866-019-1428-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6425705PMC

Characterization of ciprofloxacin-resistant and ESBL-producing Salmonella enteric serotype Derby in Eastern China.

BMC Microbiol 2019 Mar 18;19(1):61. Epub 2019 Mar 18.

Intensive Care Unit, Ningbo No. 2 Hospital, Zhejiang, Zhejiang Province, People's Republic of China.

Background: Fluoroquinolone resistance and ESBL-production are concurrently found in a limited number of Salmonella serotypes. The present study was aimed to characterize fluoroquinolone-resistant and ESBL-producing Salmonella enteric serotype Derby (S. Derby) isolates in terms of antimicrobial susceptibility, relevant genetic mechanisms, and PFGE. Read More

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http://dx.doi.org/10.1186/s12866-019-1434-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6423743PMC

Comparative evaluation of the UMIC Colistine kit to assess MIC of colistin of gram-negative rods.

BMC Microbiol 2019 Mar 18;19(1):60. Epub 2019 Mar 18.

Aix Marseille Univ, IRD, APHM, MEPHI, IHU-Méditerranée Infection, Marseille, France.

Background: The recent description of the first plasmid-mediated colistin-resistant gene mcr-1, conferring transferable and low-level resistance to colistin, raised concern about the need to implement a rapid and reliable screening method to detect colistin-resistant clinical isolates. The only valid method to assess the MIC of colistin is the broth microdilution according to the joint CLSI-EUCAST Polymyxin Breakpoints Working Group. UMIC Colistine is a ready-to-use broth microdilution kit developed to easily assess colistin MIC by proposing unitary polystyrene strips containing 11 concentrations of dehydrated colistin. Read More

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http://dx.doi.org/10.1186/s12866-019-1424-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6421643PMC

Expression profiling of genes coding for abundant proteins in the alkenone body of marine haptophyte alga Tisochrysis lutea.

Authors:
Qing Shi

BMC Microbiol 2019 Mar 11;19(1):56. Epub 2019 Mar 11.

School of Scientific Research, China University of Geosciences (Beijing), 29 Xueyuan Road, Beijing, 100083, China.

Background: Several abundant proteins have been identified in lipid body of an alkenone-producing marine haptophyte alga Tisochrysis lutea. The gene expression patterns of these proteins were investigated to better understand their roles in alkenone biosynthesis. For this purpose, T. Read More

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http://dx.doi.org/10.1186/s12866-019-1430-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6419369PMC

Letrozole treatment of adult female mice results in a similar reproductive phenotype but distinct changes in metabolism and the gut microbiome compared to pubertal mice.

BMC Microbiol 2019 Mar 12;19(1):57. Epub 2019 Mar 12.

Department of Obstetrics, Gynecology and Reproductive Sciences, University of California, San Diego, La Jolla, CA, 92093, USA.

Background: A majority of women with polycystic ovary syndrome (PCOS) have metabolic dysfunction that results in an increased risk of type 2 diabetes. We previously developed a pubertal mouse model using the aromatase inhibitor, letrozole, which recapitulates many of the reproductive and metabolic features of PCOS. To further our understanding of the effects of androgen excess, we compared the effects of letrozole treatment initiated in puberty versus adulthood on reproductive and metabolic phenotypes as well as on the gut microbiome. Read More

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http://dx.doi.org/10.1186/s12866-019-1425-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6419356PMC
March 2019
5 Reads

Epidemiology of paediatric gastrointestinal colonisation by extended spectrum cephalosporin-resistant Escherichia coli and Klebsiella pneumoniae isolates in north-west Cambodia.

BMC Microbiol 2019 Mar 12;19(1):59. Epub 2019 Mar 12.

Nuffield Department of Clinical Medicine and the National Institute for Health Research Oxford Biomedical Research Centre (NIHR-OxBRC), University of Oxford, Oxford, UK.

Background: Extended-spectrum cephalosporin resistance (ESC-R) in Escherichia coli and Klebsiella pneumoniae is a healthcare threat; high gastrointestinal carriage rates are reported from South-east Asia. Colonisation prevalence data in Cambodia are lacking. The aim of this study was to determine gastrointestinal colonisation prevalence of ESC-resistant E. Read More

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http://dx.doi.org/10.1186/s12866-019-1431-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6417137PMC

Comparative gene expression analysis of planktonic Porphyromonas gingivalis ATCC 33277 in the presence of a growing biofilm versus planktonic cells.

BMC Microbiol 2019 Mar 12;19(1):58. Epub 2019 Mar 12.

Etiology and Therapy of Periodontal Diseases (ETEP) Research Group, University Complutense, Madrid, Spain.

Background: Porphyromonas gingivalis, a microorganism residing in the oral cavity within complex multispecies biofilms, is one of the keystone pathogens in the onset and progression of periodontitis. In this in vitro study, using DNA microarray, we investigate the differential gene expression of Porphyromonas gingivalis ATCC 33277 when growing in the presence or in absence of its own monospecies biofilm.

Results: Approximately 1. Read More

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https://bmcmicrobiol.biomedcentral.com/articles/10.1186/s128
Publisher Site
http://dx.doi.org/10.1186/s12866-019-1423-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6417203PMC
March 2019
5 Reads

Transposon mutagenesis and identification of mutated genes in growth-delayed Edwardsiella ictaluri.

BMC Microbiol 2019 Mar 8;19(1):55. Epub 2019 Mar 8.

Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, MS, USA.

Background: Edwardsiella ictaluri is a Gram-negative facultative intracellular anaerobe and the etiologic agent of enteric septicemia of channel catfish (ESC). To the catfish industry, ESC is a devastating disease due to production losses and treatment costs. Identification of virulence mechanisms of E. Read More

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http://dx.doi.org/10.1186/s12866-019-1429-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6408766PMC

Cationic antimicrobial peptides: alternatives and/or adjuvants to antibiotics active against methicillin-resistant Staphylococcus aureus and multidrug-resistant Pseudomonas aeruginosa.

BMC Microbiol 2019 Mar 8;19(1):54. Epub 2019 Mar 8.

Microbiology Laboratory, School of Pharmacy, Saint Joseph University, Beirut, Lebanon.

Background: Methicillin-resistant Staphylococcus aureus and multidrug-resistant Pseudomonas aeruginosa are becoming difficult to treat with antibiotics whereas Cationic Antimicrobial Peptides (CAMPs) represent promising alternatives. The effects of four CAMPs (LL-37: human cathelicidin, CAMA: cecropin(1-7)-melittin A(2-9) amide, magainin-II and nisin) were investigated against clinical and laboratory S. aureus (n = 10) and P. Read More

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http://dx.doi.org/10.1186/s12866-019-1416-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6408789PMC

Paeniclostridium sordellii and Clostridioides difficile encode similar and clinically relevant tetracycline resistance loci in diverse genomic locations.

BMC Microbiol 2019 Mar 4;19(1):53. Epub 2019 Mar 4.

Infection and Immunity Program, Monash Biomedicine Discovery Institute and Department of Microbiology, Monash University, Clayton, Victoria, Australia.

Background: With the current rise of antibiotic resistance in bacteria, it is important to monitor the efficacy of antimicrobials in clinical use. Paeniclostridium sordellii (previously Clostridium sordellii) is a bacterial pathogen that causes human uterine infection after spontaneous or medically induced abortion, for which mortality rates approach 100%. Prophylactic antibiotics have been recommended for individuals undergoing medically-induced abortion, one of which is doxycycline, a member of the tetracycline antibiotic family. Read More

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http://dx.doi.org/10.1186/s12866-019-1427-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6399922PMC
March 2019
1 Read

Using formalin fixed paraffin embedded tissue to characterize the preterm gut microbiota in necrotising enterocolitis and spontaneous isolated perforation using marginal and diseased tissue.

BMC Microbiol 2019 Mar 4;19(1):52. Epub 2019 Mar 4.

Section of Neonatology, Department of Pediatrics, Baylor College of Medicine, Texas Children's Hospital, Houston, TX, USA.

Background: Necrotising enterocolitis (NEC) is a common cause of death in preterm infants and is closely linked to the gut microbiota. Spontaneous intestinal perforation (SIP) also occurs in preterm neonates, but results in lower mortality and less adverse neonatal outcomes than NEC. Existing studies are largely limited to non-invasive stool samples, which may not be reflective of the anatomical site of disease. Read More

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http://dx.doi.org/10.1186/s12866-019-1426-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6398254PMC
March 2019
3 Reads

Prevalence, molecular epidemiology, and antimicrobial resistance of methicillin-resistant Staphylococcus aureus from swine in southern Italy.

BMC Microbiol 2019 Feb 26;19(1):51. Epub 2019 Feb 26.

Department of Science, Roma Tre University, Viale G. Marconi 446, 00146, Rome, Italy.

Background: Colonization by livestock-associated MRSA (LA-MRSA) has increasingly been reported in the swine population worldwide. The aim of this study was to assess the prevalence of MRSA nasal carriage in healthy pigs, including the black (Calabrese) breed, from farms in the Calabria Region (Southern Italy). Between January and March 2018, a total of 475 healthy pigs reared in 32 farms were sampled by nasal swabbing. Read More

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http://dx.doi.org/10.1186/s12866-019-1422-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6390553PMC
February 2019

A coumarin analogue NFA from endophytic Aspergillus fumigatus improves drought resistance in rice as an antioxidant.

BMC Microbiol 2019 Feb 26;19(1):50. Epub 2019 Feb 26.

College of Life Science and Pharmacy, China Three Gorges University, Yichang, 443002, China.

Background: Drought and its resulting oxidative damage are the major yield limiting factors for crops in arid and semi-arid regions. Recent studies have found that endophytic fungi coexisting in plants can alleviate biotic or abiotic damage to plant growth and development. In order to screen for the endophytes associated with drought stress, 12 strains of endophytic fungi with high antioxidant activity isolated from riparian plants Myricaria laxiflora were evaluated for their effects in rice by the crude extracts. Read More

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http://dx.doi.org/10.1186/s12866-019-1419-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6390358PMC
February 2019
1 Read

Spore forming Actinobacterial diversity of Cholistan Desert Pakistan: Polyphasic taxonomy, antimicrobial potential and chemical profiling.

BMC Microbiol 2019 Feb 22;19(1):49. Epub 2019 Feb 22.

Department of Microbiology and Molecular Genetics (MMG), University of the Punjab, Quaid-e-Azam Campus, Lahore, 54590, Pakistan.

Background: Actinobacteria are famous for the production of unique secondary metabolites that help in controlling the continuously emerging drug resistance all over the globe. This study aimed at the investigation of an extreme environment the Cholistan desert, located in southern Punjab, Pakistan, for actinobacterial diversity and their activity against methicillin resistant Staphylococcus aureus (MRSA). The Cholistan desert is a sub-tropical and arid ecosystem with harsh environment, limited rainfall and low humidity. Read More

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http://dx.doi.org/10.1186/s12866-019-1414-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6387500PMC
February 2019

The application of the loop-mediated isothermal amplification (LAMP) method for diagnosing Enterococcus hirae-associated endocarditis outbreaks in chickens.

BMC Microbiol 2019 Feb 21;19(1):48. Epub 2019 Feb 21.

Department of Pathology and Veterinary Diagnostics, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Nowoursynowska 159c, 02-776, Warsaw, Poland.

Background: Enterococcus hirae is considered a part of the normal intestinal biota of several domestic animals, including poultry. However, this species is also associated with infective endocarditis in chickens, a disease that leads to unexpected deaths and serious economical losses. Enterococcus hirae is identified predominantly with the use of conventional bacteriological methods, biochemical tests and PCR. Read More

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http://dx.doi.org/10.1186/s12866-019-1420-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6385395PMC
February 2019
1 Read

Age-related variation in the oral microbiome of urban Cooper's hawks (Accipiter cooperii).

BMC Microbiol 2019 Feb 21;19(1):47. Epub 2019 Feb 21.

School of Plant Sciences, The University of Arizona, Tucson, USA.

Background: Bird species worldwide are affected by trichomoniasis caused by the protist Trichomonas gallinae. In avivorous raptors such as Cooper's hawks (Accipiter cooperii), nestlings are more susceptible than fledglings and adults. Previous research suggested a link between oral pH and susceptibility: the oral pH of fledgling and adult hawks is more than seven times more acidic than that of nestlings. Read More

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http://dx.doi.org/10.1186/s12866-019-1413-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6385412PMC
February 2019

Genome plasticity favours double chromosomal Tn4401b-bla transposon insertion in the Pseudomonas aeruginosa ST235 clone.

BMC Microbiol 2019 Feb 20;19(1):45. Epub 2019 Feb 20.

Bacterial Molecular Genetics Laboratory, Universidad El Bosque, Carrera 9 N°131A-02, Bogotá D.C, Colombia.

Background: Pseudomonas aeruginosa Sequence Type 235 is a clone that possesses an extraordinary ability to acquire mobile genetic elements and has been associated with the spread of resistance genes, including genes that encode for carbapenemases. Here, we aim to characterize the genetic platforms involved in resistance dissemination in bla-positive P. aeruginosa ST235 in Colombia. Read More

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http://dx.doi.org/10.1186/s12866-019-1418-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6381643PMC
February 2019
1 Read

Functional analysis of RIP toxins from the Drosophila endosymbiont Spiroplasma poulsonii.

BMC Microbiol 2019 Feb 20;19(1):46. Epub 2019 Feb 20.

Global Health Institute, School of Life Science, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland.

Background: Insects frequently live in close relationship with symbiotic bacteria that carry out beneficial functions for their host, like protection against parasites and viruses. However, in some cases, the mutualistic nature of such associations is put into question because of detrimental phenotypes caused by the symbiont. One example is the association between the vertically transmitted facultative endosymbiont Spiroplasma poulsonii and its natural host Drosophila melanogaster. Read More

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http://dx.doi.org/10.1186/s12866-019-1410-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6383259PMC
February 2019

Purification and characterization of actinomycins from Streptomyces strain M7 active against methicillin resistant Staphylococcus aureus and vancomycin resistant Enterococcus.

BMC Microbiol 2019 Feb 19;19(1):44. Epub 2019 Feb 19.

Department of Microbiology, Guru Nanak Dev University, Amritsar, Punjab, 143005, India.

Background: The increased rate of resistance among two highly concerned pathogens i.e. methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococcus (VRE) necessitates the discovery of novel anti-MRSA and anti-VRE compounds. Read More

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http://dx.doi.org/10.1186/s12866-019-1405-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6381723PMC
February 2019
1 Read

Sensitive and immunogen-specific serological detection of Rodentibacter pneumotropicus infections in mice.

BMC Microbiol 2019 Feb 18;19(1):43. Epub 2019 Feb 18.

Institute of Bioanalytical Chemistry, Faculty of Chemistry and Mineralogy, Universität Leipzig, Leipzig, Germany.

Background: Rodentibacter (R.) pneumotropicus colonizes the respiratory and urogenital tracts of laboratory mice with a reported moderate serological prevalence from 4 to 13%. Thus, regular tests to identify this pathogen in mice are recommended for animal facilities. Read More

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http://dx.doi.org/10.1186/s12866-019-1417-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6380038PMC
February 2019
2 Reads

Gut microbiome patterns correlate with higher postoperative complication rates after pancreatic surgery.

BMC Microbiol 2019 Feb 18;19(1):42. Epub 2019 Feb 18.

Department of Infectious Diseases, Medical Microbiology and Hygiene, Heidelberg University Hospital, Heidelberg, Germany.

Background: Postoperative complications are of great relevance in daily clinical practice, and the gut microbiome might play an important role by preventing pathogens from crossing the intestinal barrier. The two aims of this prospective clinical pilot study were: (1) to examine changes in the gut microbiome following pancreatic surgery, and (2) to correlate these changes with the postoperative course of the patient.

Results: In total, 116 stool samples of 32 patients undergoing pancreatic surgery were analysed by 16S-rRNA gene next-generation sequencing. Read More

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http://dx.doi.org/10.1186/s12866-019-1399-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6379976PMC
February 2019

Ability of the So-Called Obligate Hydrocarbonoclastic Bacteria to Utilize Nonhydrocarbon Substrates Thus Enhancing Their Activities Despite their Misleading Name.

BMC Microbiol 2019 Feb 18;19(1):41. Epub 2019 Feb 18.

Microbiology program, Department of Biological Sciences, Faculty of Science, Kuwait University, P.O. Box 5969, 13060, Safat, Kuwait.

Background: The group of the so-called obligate hydrocarbonoclastic bacteria (OHCB) are marine microorganisms affiliated with the genera Alcanivorax, Cycloclasticus, Oleiphilus and Thalassolituus. This small group plays a major role in oil-bioremediation in marine ecosystems. Marinobacter and Planomicrobium are considered related to this group. Read More

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http://dx.doi.org/10.1186/s12866-019-1406-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6379940PMC
February 2019
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Characterization of enzymatic properties of two novel enzymes, 3,4-dihydroxyphenylacetate dioxygenase and 4-hydroxyphenylacetate 3-hydroxylase, from Sulfobacillus acidophilus TPY.

BMC Microbiol 2019 Feb 13;19(1):40. Epub 2019 Feb 13.

Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen, 361005, Fujian, China.

Background: As an environmental pollutant, 4-hydroxyphenylacetate (4-HPA) was a product of softwood lignin decomposition and was found in industrial effluents from olive oil production. Sulfobacillus acidophilus TPY was a moderately thermoacidophilic bacterium capable of degrading aromatic compounds including 4-HPA. The enzymes involved in the degradation of 4-HPA and the role of this strain in the bioremediation of marine pollutants need to be illustrated. Read More

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http://dx.doi.org/10.1186/s12866-019-1415-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6375179PMC
February 2019

Inhibition of pathogenic bacterial biofilms on PDMS based implants by L. acidophilus derived biosurfactant.

BMC Microbiol 2019 Feb 13;19(1):39. Epub 2019 Feb 13.

Department of Physics, Savitribai Phule Pune University, Pune, Maharashtra, 411007, India.

Background: Lactobacillus spp. predominantly shows its presence as a normal mucosal flora of the mouth and intestine. Therefore, the objective of our research is to investigate the in-vitro conditions for the prospective of medically valuable biosurfactants (BSs) derived from Lactobacillus spp. Read More

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http://dx.doi.org/10.1186/s12866-019-1412-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6374892PMC
February 2019
2 Reads
2.729 Impact Factor

Mangrove derived Streptomyces sp. MUM265 as a potential source of antioxidant and anticolon-cancer agents.

BMC Microbiol 2019 Feb 13;19(1):38. Epub 2019 Feb 13.

Biofunctional Molecule Exploratory (BMEX) Research Group, School of Pharmacy, Monash University Malaysia, 47500, Bandar Sunway, Selangor Darul Ehsan, Malaysia.

Background: Colon cancer is the third most commonly diagnosed cancer worldwide, with a commensurately high mortality rate. The search for novel antioxidants and specific anticancer agents which may inhibit, delay or reverse the development of colon cancer is thus an area of great interest; Streptomyces bacteria have been demonstrated to be a source of such agents.

Results: The extract from Streptomyces sp. Read More

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http://dx.doi.org/10.1186/s12866-019-1409-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6375222PMC
February 2019
2.729 Impact Factor

Application of qPCR assays based on haloacids transporter gene dehp2 for discrimination of Burkholderia and Paraburkholderia.

BMC Microbiol 2019 Feb 11;19(1):36. Epub 2019 Feb 11.

Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University, Shanghai, China.

Background: A major facilitator superfamily transporter Dehp2 was recently shown to be playing an important role in transport and biodegradation of haloacids in Paraburkholderia caribensis MBA4, and Dehp2 is phylogenetically conserved in Burkholderia sensu lato.

Results: We designed both Burkholderia sensu stricto-specific and Paraburkholderia-specific qPCR assays based on dehp2 and 16S rRNA, and validated the qPCR assays in 12 bacterial strains. The qPCR assays could detect single species of Burkholderia sensu stricto or Paraburkholderia with high sensitivity and discriminate them in mixtures with high specificity over a wide dynamic range of relative concentrations. Read More

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http://dx.doi.org/10.1186/s12866-019-1411-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6371555PMC
February 2019
1 Read

Biological characterization of Pasteurella multocida present in the Saiga population.

BMC Microbiol 2019 Feb 11;19(1):37. Epub 2019 Feb 11.

Royal Veterinary College, Hawkshead Lane, Hatfield, Herts, AL9 7TA, UK.

Background: This study provides biochemical and molecular genetic characteristics of P. multocida isolated from dead saigas in 1988, 2010-2015 on the territory of the Republic of Kazakhstan.

Results: Bacteriological samples taken from carcasses of saiga antelope during mortality events recorded in West Kazakhstan in both 2010 and 2011 and in Kostanay in 2012 and 2015 confirmed the presence of P. Read More

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https://bmcmicrobiol.biomedcentral.com/articles/10.1186/s128
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http://dx.doi.org/10.1186/s12866-019-1407-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6371526PMC
February 2019
2 Reads

The study of metabolites from fermentation culture of Alternaria oxytropis.

BMC Microbiol 2019 Feb 11;19(1):35. Epub 2019 Feb 11.

College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, Shaanxi, China.

Background: The indolizidine alkaloid-swainsonine is produced by an endophytic fungus Alternaria oxytropis, which was isolated from locoweeds. Swainsonine has many biological activities such as anti-tumorigenic, anti-viral and bacteriostatic. However, the full complement of metabolites produced by Alternaria oxytropis is not known. Read More

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http://dx.doi.org/10.1186/s12866-019-1408-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6369557PMC
February 2019
2 Reads

Osteopontin in the host response to Leishmania amazonensis.

BMC Microbiol 2019 Feb 8;19(1):32. Epub 2019 Feb 8.

Immunophysiology and Parasitism Laboratory and Department of Parasites and Insect Vectors, Institut Pasteur, 28 rue du Dr Roux, 75724, Paris Cedex 15, France.

Background: Leishmania (L.) spp are intracellular eukaryotic parasites responsible for cutaneous or visceral leishmaniasis, replicating predominantly in macrophages (MF). In C57BL/6 mice virulence with L. Read More

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http://dx.doi.org/10.1186/s12866-019-1404-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6368773PMC
February 2019

Lactobacillus mucosae DPC 6426 as a bile-modifying and immunomodulatory microbe.

BMC Microbiol 2019 Feb 8;19(1):33. Epub 2019 Feb 8.

Teagasc Food Research Centre, Food Biosciences Department, Moorepark, Fermoy, Co, Cork, Ireland.

Background: Lactobacillus mucosae DPC 6426 has previously demonstrated potentially cardio-protective properties, in the form of dyslipidaemia and hypercholesterolemia correction in an apolipoprotein-E deficient mouse model. This study aims to characterise the manner in which this microbe may modulate host bile pool composition and immune response, in the context of cardiovascular disease. Lactobacillus mucosae DPC 6426 was assessed for bile salt hydrolase activity and specificity. Read More

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http://dx.doi.org/10.1186/s12866-019-1403-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6368806PMC
February 2019
1 Read
2.729 Impact Factor

Single stranded (ss)RNA-mediated antiviral response against infectious laryngotracheitis virus infection.

BMC Microbiol 2019 Feb 8;19(1):34. Epub 2019 Feb 8.

Faculty of Veterinary Medicine, Health Research Innovation Center 2C53, University of Calgary, 3330 Hospital Drive NW, Calgary, AB, T2N 4N1, Canada.

Background: Single stranded ribonucleic acid (ssRNA) binds to toll-like receptor (TLR)7 leading to recruitment of immune cells and production of pro-inflammatory cytokines, which has been shown in mammals. In chickens, synthetic ssRNA analog, resiquimod, has been shown to elicit antiviral response against infectious bursal disease virus infection. The objective of this study was to determine the innate host responses activated by the pre-hatch in ovo administration of resiquimod against infectious laryngotracheitis virus (ILTV) infection in chickens post-hatch. Read More

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https://bmcmicrobiol.biomedcentral.com/articles/10.1186/s128
Publisher Site
http://dx.doi.org/10.1186/s12866-019-1398-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6368756PMC
February 2019
13 Reads

Phylogenetic relationship of Ornithobacterium rhinotracheale isolated from poultry and diverse avian hosts based on 16S rRNA and rpoB gene analyses.

BMC Microbiol 2019 Feb 6;19(1):31. Epub 2019 Feb 6.

Department of Wildlife Diseases, Leibniz Institute for Zoo and Wildlife Research, Berlin, Germany.

Background: Ornithobacterium (O.) rhinotracheale is an emerging bacterial pathogen in poultry and not fully understood to date. Because of its importance particularly for the global turkey meat industry, reliable diagnostic and characterization methods are needed for early treatment and in future for better vaccine production. Read More

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http://dx.doi.org/10.1186/s12866-019-1395-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6364391PMC
February 2019
2.729 Impact Factor

Ruminal metagenomic analyses of goat data reveals potential functional microbiota by supplementation with essential oil-cobalt complexes.

BMC Microbiol 2019 Feb 4;19(1):30. Epub 2019 Feb 4.

College of Animal Science and Technology, Gansu Agriculture University, Lanzhou, 730070, China.

Background: Essential Oils (EO) are complex mixtures of plant secondary metabolites that have been proposed as promising feed additives for mitigating methane and ammonia emissions. We have previously demonstrated that Essential Oil-Cobalt (EOC) supplementation resulted in increased average daily gain and improved phenotypes (cashmere fiber traits, carcass weight, and meat quality) when cashmere goats received supplementation at approximately 2 mg/kg of body weight. However, the ruminal microbiological effects of EO remain poorly understood with regard to the extent to which ruminal populations can adapt to EO presence as feed ingredients. Read More

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http://dx.doi.org/10.1186/s12866-019-1400-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6362596PMC
February 2019
3 Reads