3,977 results match your criteria BMC Microbiology[Journal]


Characterization of a potential probiotic bacterium Lactococcus raffinolactis WiKim0068 isolated from fermented vegetable using genomic and in vitro analyses.

BMC Microbiol 2020 May 27;20(1):136. Epub 2020 May 27.

Microbiology and Functionality Research Group, World Institute of Kimchi, Gwangju, 61755, Republic of Korea.

Background: Lactococcus members belonging to lactic acid bacteria are widely used as starter bacteria in the production of fermented dairy products. From kimchi, a Korean food made of fermented vegetables, Lactococcus raffinolactis WiKim0068 was isolated and its genome was analyzed.

Results: The complete genome of the strain WiKim0068 consists of one chromosome and two plasmids that comprises 2,292,235 bp, with a G + C content of 39. Read More

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http://dx.doi.org/10.1186/s12866-020-01820-9DOI Listing

Effect of donor-recipient relatedness on the plasmid conjugation frequency: a meta-analysis.

BMC Microbiol 2020 May 26;20(1):135. Epub 2020 May 26.

Department of Population Health Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands.

Background: Conjugation plays a major role in the transmission of plasmids encoding antibiotic resistance genes in both clinical and general settings. The conjugation efficiency is influenced by many biotic and abiotic factors, one of which is the taxonomic relatedness between donor and recipient bacteria. A comprehensive overview of the influence of donor-recipient relatedness on conjugation is still lacking, but such an overview is important to quantitatively assess the risk of plasmid transfer and the effect of interventions which limit the spread of antibiotic resistance, and to obtain parameter values for conjugation in mathematical models. Read More

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http://dx.doi.org/10.1186/s12866-020-01825-4DOI Listing

Nanopore sequencing reveals genomic map of CTX-M-type extended-spectrum β-lactamases carried by Escherichia coli strains isolated from blue mussels (Mytilus edulis) in Norway.

BMC Microbiol 2020 May 25;20(1):134. Epub 2020 May 25.

Department of Contaminants and Biohazards, Institute of Marine Research (IMR), Bergen, Norway.

Background: Environmental surveillance of antibiotic resistance can contribute towards better understanding and management of human and environmental health. This study applied a combination of long-read Oxford Nanopore MinION and short-read Illumina MiSeq-based sequencing to obtain closed complete genome sequences of two CTX-M-producing multidrug-resistant Escherichia coli strains isolated from blue mussels (Mytilus edulis) in Norway, in order to understand the potential for mobility of the detected antibiotic resistance genes (ARGs).

Results: The complete genome sequence of strain 631 (E. Read More

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http://dx.doi.org/10.1186/s12866-020-01821-8DOI Listing

LipF increases rifampicin and streptomycin sensitivity in a Mycobacterium tuberculosis surrogate.

BMC Microbiol 2020 May 25;20(1):132. Epub 2020 May 25.

Departamento de Biología Molecular, Centro de Investigación Biomédica del Noreste, Instituto Mexicano del Seguro Social, Monterrey, Nuevo León, México.

Background: Mortality due to tuberculosis (TB) has increased due to the development of drug resistance, the mechanisms of which have not been fully elucidated. Our research group identified a low expression of lipF gene in Mycobacterium tuberculosis clinical isolates with drug resistance. The aim of this work was to evaluate the effect of lipase F (LipF) expression on mycobacterial drug resistance. Read More

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http://dx.doi.org/10.1186/s12866-020-01802-xDOI Listing

In vitro conjugation kinetics of AmpC, broad spectrum and extended-spectrum beta-lactamase-producing Escherichia coli donors and various Enterobacteriaceae recipients.

BMC Microbiol 2020 May 25;20(1):133. Epub 2020 May 25.

Institute of Animal Nutrition, Freie Universität Berlin, Königin-Luise-Str. 49, 14195, Berlin, Germany.

Background: Extended spectrum beta-lactamase (ESBL)-producing enterobacteria pose a major hazard to public health. Due to the possibility of genetic transfer, ESBL genes might spread to pathogenic enterobacterial strains. Thus, information on possible genetic transfer between enterobacteria is of high interest. Read More

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http://dx.doi.org/10.1186/s12866-020-01787-7DOI Listing

Assessment of real-time PCR for Helicobacter pylori DNA detection in stool with co-infection of intestinal parasites: a comparative study of DNA extraction methods.

BMC Microbiol 2020 May 24;20(1):131. Epub 2020 May 24.

Department of Infectious-Tropical Diseases and Microbiology, IRCCS Sacro Cuore Don Calabria Hospital, Via Don A. Sempreboni, 5 - 37024 Negrar di Valpolicella, Verona, Italy.

Background: Many studies reported high prevalence of H. pylori infection among patients co-infected with intestinal parasites. Molecular approach for the DNA detection of those microbes in stool have been proposed. Read More

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http://dx.doi.org/10.1186/s12866-020-01824-5DOI Listing

Discovery of 20 novel ribosomal leader candidates in bacteria and archaea.

BMC Microbiol 2020 May 24;20(1):130. Epub 2020 May 24.

Bioinformatics Group, Department of Computer Science and Interdisciplinary Centre for Bioinformatics, Leipzig University, Härtelstraße 16-18, 04107, Leipzig, Germany.

Background: RNAs perform many functions in addition to supplying coding templates, such as binding proteins. RNA-protein interactions are important in multiple processes in all domains of life, and the discovery of additional protein-binding RNAs expands the scope for studying such interactions. To find such RNAs, we exploited a form of ribosomal regulation. Read More

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http://dx.doi.org/10.1186/s12866-020-01823-6DOI Listing

Efficient dual-negative selection for bacterial genome editing.

BMC Microbiol 2020 May 24;20(1):129. Epub 2020 May 24.

Biozentrum, University of Basel, CH-4056, Basel, Switzerland.

Background: Gene editing is key for elucidating gene function. Traditional methods, such as consecutive single-crossovers, have been widely used to modify bacterial genomes. However, cumbersome cloning and limited efficiency of negative selection often make this method slower than other methods such as recombineering. Read More

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http://dx.doi.org/10.1186/s12866-020-01819-2DOI Listing

Profiles of Staphyloccocus aureus isolated from goat persistent mastitis before and after treatment with enrofloxacin.

BMC Microbiol 2020 May 24;20(1):127. Epub 2020 May 24.

Laboratory of Bacterial Diseases (LDBAC), Veterinary Department, Universidade Federal de Vicosa (UFV), PH Rolfs Avenue, s/n, University Campus, Viçosa, Minas Gerais, 36570-900, Brazil.

Background: Staphylococcus aureus is one of the main causative agents of mastitis in small ruminants. Antimicrobial use is the major treatment, but there are many flaws linked to resistance, tolerance or persistence. This study aimed to verify changes in resistance, virulence and clonal profiles of S. Read More

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http://dx.doi.org/10.1186/s12866-020-01793-9DOI Listing

Hydrogen peroxide-producing pyruvate oxidase from Lactobacillus delbrueckii is catalytically activated by phosphotidylethanolamine.

BMC Microbiol 2020 May 24;20(1):128. Epub 2020 May 24.

Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, MA, 02111, USA.

Background: Pyruvate oxidase (Pox) is an important enzyme in bacterial metabolism for increasing ATP production and providing a fitness advantage via hydrogen peroxide production. However, few Pox enzymes have been characterized from bacterial species. The tetrameric non-hydrogen-peroxide producing Pox from E. Read More

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http://dx.doi.org/10.1186/s12866-020-01788-6DOI Listing

New perspectives on butyrate assimilation in Rhodospirillum rubrum S1H under photoheterotrophic conditions.

BMC Microbiol 2020 May 20;20(1):126. Epub 2020 May 20.

Laboratory of Proteomics and Microbiology, Research Institute for Biosciences, University of Mons, Avenue du champs de Mars, 6 (Pentagone 3B), 7000, Mons, Belgium.

Background: The great metabolic versatility of the purple non-sulfur bacteria is of particular interest in green technology. Rhodospirillum rubrum S1H is an α-proteobacterium that is capable of photoheterotrophic assimilation of volatile fatty acids (VFAs). Butyrate is one of the most abundant VFAs produced during fermentative biodegradation of crude organic wastes in various applications. Read More

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http://dx.doi.org/10.1186/s12866-020-01814-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7238569PMC

Viral metagenomic analysis of fecal samples reveals an enteric virome signature in irritable bowel syndrome.

BMC Microbiol 2020 May 19;20(1):123. Epub 2020 May 19.

Health Policy Research Center, Institute of Health, Shiraz University of Medical Sciences, Shiraz, Iran.

Background: Changes in the enteric microbiota have been suggested to contribute to gastrointestinal diseases, including irritable bowel syndrome. Most of the published work is on bacterial dysbiosis with meager data on the role of the virome in irritable bowel syndrome and other gastrointestinal diseases. In the current study, we therefore aimed to investigate the viral community composition of the gut and test for potential dysbiosis linked to irritable bowel syndrome. Read More

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http://dx.doi.org/10.1186/s12866-020-01817-4DOI Listing

Antimicrobial sensitivity profile and bacterial isolates among suspected pyogenic meningitis patients attending at Hawassa University Hospital: Cross-sectional study.

BMC Microbiol 2020 May 19;20(1):125. Epub 2020 May 19.

Department of Neurology, Hawassa University College of Medicine and Health Sciences, Hawassa, Ethiopia.

Background: Bacterial meningitis is a serious inflammation of the meninges. Antimicrobial therapy on early cerebrospinal fluid (CSF) examination has an important role in diagnosis. The disease is still challenging in developing countries because of poor (diagnostic set-up, socioeconomic conditions, management), and misuse of antimicrobial therapy results in emerging antimicrobial-resistant strains. Read More

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http://dx.doi.org/10.1186/s12866-020-01808-5DOI Listing

Proteome profile changes during poly-hydroxybutyrate intracellular mobilization in gram positive Bacillus cereus tsu1.

BMC Microbiol 2020 May 19;20(1):122. Epub 2020 May 19.

R.W. Holley Center for Agriculture and Health, USDA-ARS, Cornell University, Ithaca, NY, 14853, USA.

Background: Bacillus cereus is a bacterial species which grows efficiently on a wide range of carbon sources and accumulates biopolymer poly-hydroxybutyrate (PHB) up to 80% cell dry weight. PHB is an aliphatic polymer produced and stored intracellularly as a reservoir of carbon and energy, its mobilization is a key biological process for sporulation in Bacillus spp. Previously, B. Read More

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http://dx.doi.org/10.1186/s12866-020-01815-6DOI Listing

A novel gene expression system for Ralstonia eutropha based on the T7 promoter.

BMC Microbiol 2020 May 19;20(1):121. Epub 2020 May 19.

Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, People's Republic of China.

Background: Ralstonia eutropha (syn. Cupriavidus necator) is a model microorganism for studying metabolism of polyhydroxyalkanoates (PHAs) and a potential chassis for protein expression due to various advantages. Although current plasmid systems of R. Read More

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http://dx.doi.org/10.1186/s12866-020-01812-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7236105PMC

Uncovering the role of Symbiodiniaceae assemblage composition and abundance in coral bleaching response by minimizing sampling and evolutionary biases.

BMC Microbiol 2020 May 19;20(1):124. Epub 2020 May 19.

Department of Civil and Environmental Engineering, Northwestern University, Evanston, IL, 60208, USA.

Background: Biodiversity and productivity of coral-reef ecosystems depend upon reef-building corals and their associations with endosymbiotic Symbiodiniaceae, which offer diverse functional capabilities to their hosts. The number of unique symbiotic partners (richness) and relative abundances (evenness) have been hypothesized to affect host response to climate change induced thermal stress. Symbiodiniaceae assemblages with many unique phylotypes may provide greater physiological flexibility or form less stable symbioses; assemblages with low abundance phylotypes may allow corals to retain thermotolerant symbionts or represent associations with less-suitable symbionts. Read More

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http://dx.doi.org/10.1186/s12866-020-01765-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7236918PMC

Defining the oral microbiome by whole-genome sequencing and resistome analysis: the complexity of the healthy picture.

BMC Microbiol 2020 May 18;20(1):120. Epub 2020 May 18.

Section of Dentistry, Department of Biomedical and Specialty Surgical Sciences, University of Ferrara, Ferrara, Italy.

Background: The microbiome of the oral cavity is the second-largest and diverse microbiota after the gut, harboring over 700 species of bacteria and including also fungi, viruses, and protozoa. With its diverse niches, the oral cavity is a very complex environment, where different microbes preferentially colonize different habitats. Recent data indicate that the oral microbiome has essential functions in maintaining oral and systemic health, and the emergence of 16S rRNA gene next-generation sequencing (NGS) has greatly contributed to revealing the complexity of its bacterial component. Read More

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http://dx.doi.org/10.1186/s12866-020-01801-yDOI Listing

Metabolic diversity and co-occurrence of multiple Ferrovum species at an acid mine drainage site.

BMC Microbiol 2020 May 18;20(1):119. Epub 2020 May 18.

Department of Plant and Microbial Biology, University of Minnesota, 218 Cargill Building, St. Paul, MN, 55108, USA.

Background: Ferrovum spp. are abundant in acid mine drainage sites globally where they play an important role in biogeochemical cycling. All known taxa in this genus are Fe(II) oxidizers. Read More

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http://dx.doi.org/10.1186/s12866-020-01768-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7236192PMC

Growth inhibition and metabolomic analysis of Xanthomonas oryzae pv. oryzae treated with resveratrol.

BMC Microbiol 2020 May 14;20(1):117. Epub 2020 May 14.

School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing, 210094, China.

Background: Xanthomonas oryzae pv. oryzae (Xoo) can cause destructive bacterial blight in rice. As an antibacterial, resveratrol may inhibit Xoo growth. Read More

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http://dx.doi.org/10.1186/s12866-020-01803-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7227335PMC

In vitro synergistic potentials of novel antibacterial combination therapies against Salmonella enterica serovar Typhimurium.

BMC Microbiol 2020 May 14;20(1):118. Epub 2020 May 14.

Veterinary Drugs & Biologics Division, Animal and Plant Quarantine Agency, Gimcheon-si, 39660, Republic of Korea.

Background: The antibiotics generally used in farm animals are rapidly losing their effectiveness all over the world as bacteria develop antibiotic resistance. Like some other pathogenic bacteria multidrug-resistant strains of Salmonella enterica serovar Typhimurium (S. Typhimurium) are also frequently found in animals and humans which poses a major public health concern. Read More

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http://dx.doi.org/10.1186/s12866-020-01810-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7227263PMC

Dynamic distribution of gut microbiota in meat rabbits at different growth stages and relationship with average daily gain (ADG).

BMC Microbiol 2020 May 14;20(1):116. Epub 2020 May 14.

College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.

Background: The mammalian intestinal tract harbors diverse and dynamic microbial communities that play pivotal roles in host health, metabolism, immunity, and development. Average daily gain (ADG) is an important growth trait in meat rabbit industry. The effects of gut microbiota on ADG in meat rabbits are still unknown. Read More

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http://dx.doi.org/10.1186/s12866-020-01797-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7227296PMC

The exopolysaccharide-eDNA interaction modulates 3D architecture of Bacillus subtilis biofilm.

BMC Microbiol 2020 May 14;20(1):115. Epub 2020 May 14.

State Key Laboratory of Agricultural Microbiology, College of Resources of Environment, Huazhong Agricultural University, Wuhan, 430070, China.

Background: Bacterial biofilms are surface-adherent microbial communities in which individual cells are surrounded by a self-produced extracellular matrix of polysaccharides, extracellular DNA (eDNA) and proteins. Interactions among matrix components within biofilms are responsible for creating an adaptable structure during biofilm development. However, it is unclear how the interactions among matrix components contribute to the construction of the three-dimensional (3D) biofilm architecture. Read More

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http://dx.doi.org/10.1186/s12866-020-01789-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7227074PMC
May 2020
2.729 Impact Factor

Discovery of thermophilic Bacillales using reduced-representation genotyping for identification.

BMC Microbiol 2020 May 13;20(1):114. Epub 2020 May 13.

Institute of Applied Ecology, University of Canberra, Canberra, ACT, 2601, Australia.

Background: This study demonstrates the use of reduced-representation genotyping to provide preliminary identifications for thermophilic bacterial isolates. The approach combines restriction enzyme digestion and PCR with next-generation sequencing to provide thousands of short-read sequences from across the bacterial genomes. Isolates were obtained from compost, hot water systems, and artesian bores of the Great Artesian Basin. Read More

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http://dx.doi.org/10.1186/s12866-020-01800-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7222431PMC

Morin inhibits Listeria monocytogenes virulence in vivo and in vitro by targeting listeriolysin O and inflammation.

BMC Microbiol 2020 May 12;20(1):112. Epub 2020 May 12.

Department of Respiratory Medicine, The First Hospital of Jilin University, Jilin University, Changchun, Jilin, 130021, China.

Background: Listeria monocytogenes (L. monocytogenes) is a global opportunistic intracellular pathogen that can cause many infections, including meningitis and abortion in humans and animals; thus, L. monocytogenes poses a great threat to public safety and the development of the aquaculture industry. Read More

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http://dx.doi.org/10.1186/s12866-020-01807-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7216731PMC

Optimizing 16S rRNA gene profile analysis from low biomass nasopharyngeal and induced sputum specimens.

BMC Microbiol 2020 May 12;20(1):113. Epub 2020 May 12.

Division of Medical Microbiology, Department of Pathology, Faculty of Health Sciences, University of Cape Town, Cape Town, South Africa.

Background: Careful consideration of experimental artefacts is required in order to successfully apply high-throughput 16S ribosomal ribonucleic acid (rRNA) gene sequencing technology. Here we introduce experimental design, quality control and "denoising" approaches for sequencing low biomass specimens.

Results: We found that bacterial biomass is a key driver of 16S rRNA gene sequencing profiles generated from bacterial mock communities and that the use of different deoxyribonucleic acid (DNA) extraction methods [DSP Virus/Pathogen Mini Kit® (Kit-QS) and ZymoBIOMICS DNA Miniprep Kit (Kit-ZB)] and storage buffers [PrimeStore® Molecular Transport medium (Primestore) and Skim-milk, Tryptone, Glucose and Glycerol (STGG)] further influence these profiles. Read More

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http://dx.doi.org/10.1186/s12866-020-01795-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7218582PMC

Bifidobacterium mongoliense genome seems particularly adapted to milk oligosaccharide digestion leading to production of antivirulent metabolites.

BMC Microbiol 2020 May 7;20(1):111. Epub 2020 May 7.

Department of Food Science, Fundamental and Applied Research for Animal and Health, Faculty of Veterinary Medicine, University of Liège, Liège, Belgium.

Background: Human milk oligosaccharides (HMO) could promote the growth of bifidobacteria, improving young children's health. In addition, fermentation of carbohydrates by bifidobacteria can result in the production of metabolites presenting an antivirulent activity against intestinal pathogens. Bovine milk oligosaccharides (BMO), structurally similar to HMO, are found at high concentration in cow whey. Read More

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http://dx.doi.org/10.1186/s12866-020-01804-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7206731PMC

The model cyanobacteria Anabaena sp. PCC 7120 possess an intact but partially degenerated gene cluster encoding gas vesicles.

BMC Microbiol 2020 May 6;20(1):110. Epub 2020 May 6.

Hefei National Laboratory for Physical Sciences at the Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, 230027, Anhui, China.

Background: Bacterial gas vesicles, composed of two major gas vesicle proteins and filled with gas, are a unique class of intracellular bubble-like nanostructures. They provide buoyancy for cells, and thus play an essential role in the growth and survival of aquatic and soil microbes. Moreover, the gas vesicle could be applied to multimodal and noninvasive biological imaging as a potential nanoscale contrast agent. Read More

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http://dx.doi.org/10.1186/s12866-020-01805-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7204071PMC

A single amino acid substitution in aromatic hydroxylase (HpaB) of Escherichia coli alters substrate specificity of the structural isomers of hydroxyphenylacetate.

BMC Microbiol 2020 May 6;20(1):109. Epub 2020 May 6.

Department of Bioscience and Biotechnology, Konkuk University, Seoul, 05029, Republic of Korea.

Background: A broad range of aromatic compounds can be degraded by enteric bacteria, and hydroxyphenylacetic acid (HPA) degrading bacteria are the most widespread. Majority of Escherichia coli strains can use both the structural isomers of HPA, 3HPA and 4HPA, as the sole carbon source, which are catabolized by the same pathway whose associated enzymes are encoded by hpa gene cluster. Previously, we observed that E. Read More

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http://dx.doi.org/10.1186/s12866-020-01798-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7201708PMC
May 2020
2.729 Impact Factor

Insight to shape of soil microbiome during the ternary cropping system of Gastradia elata.

BMC Microbiol 2020 May 5;20(1):108. Epub 2020 May 5.

Guizhou University of Traditional Chinese Medicine, Dongqingnan Road, Guiyang, 540025, Guizhou, China.

Background: The ternary cropping system of Gastradia elata depends on a symbiotic relationship with the mycorrhizal fungi Armillaria mellea, which decays wood to assimilate nutrition for the growth of G. elata. The composition of microbe flora as key determinants of rhizoshere and mycorrhizoshere soil fertility and health was investigated to understand how G. Read More

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http://dx.doi.org/10.1186/s12866-020-01790-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7201697PMC

Genomic heterogeneity of Dichelobacter nodosus within and between UK sheep flocks and between age groups within a flock.

BMC Microbiol 2020 May 1;20(1):107. Epub 2020 May 1.

University of Nottingham, School of Veterinary Medicine and Science, Sutton Bonington, UK.

Background: Footrot and interdigital dermatitis are endemic infectious diseases in all sheep farming regions, impairing welfare and production. The development of efficacious vaccines against the primary causative pathogen has been hampered by the extensive antigenic diversity of Dichelobacter nodosus. Understanding the heterogeneity of the pathogen within and between flocks is essential if the feasibility of bespoke vaccine production is to be assessed for use in the U. Read More

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http://dx.doi.org/10.1186/s12866-020-01769-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7193352PMC

Complete genome sequencing and comparative genomic analyses of Bacillus sp. S3, a novel hyper Sb(III)-oxidizing bacterium.

BMC Microbiol 2020 May 1;20(1):106. Epub 2020 May 1.

School of Minerals Processing and Bioengineering, Central South University, Changsha, 410083, China.

Background: Antimonite [Sb(III)]-oxidizing bacterium has great potential in the environmental bioremediation of Sb-polluted sites. Bacillus sp. S3 that was previously isolated from antimony-contaminated soil displayed high Sb(III) resistance and Sb(III) oxidation efficiency. Read More

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http://dx.doi.org/10.1186/s12866-020-01737-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7193398PMC

Correction to: Identification of cholesterol-assimilating actinomycetes strain and application of statistical modeling approaches for improvement of cholesterol oxidase production by Streptomyces anulatus strain NEAE-94.

BMC Microbiol 2020 Apr 30;20(1):105. Epub 2020 Apr 30.

Department of Bioprocess Development, Genetic Engineering and Biotechnology Research Institute, City for Scientific Research and Technological Applications, Alexandria, Egypt.

Following publication of the original article [1], the authors reported an error in Fig. 2c. Read More

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http://dx.doi.org/10.1186/s12866-020-01791-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7191723PMC
April 2020
2.729 Impact Factor

Low phosphatase activity of LiaS and strong LiaR-DNA affinity explain the unusual LiaS to LiaR in vivo stoichiometry.

BMC Microbiol 2020 Apr 29;20(1):104. Epub 2020 Apr 29.

Department of Biology, York University, Toronto, ON, M3J1P3, Canada.

Background: LiaRS mediates Bacillus subtilis response to cell envelope perturbations. A third protein, LiaF, has an inhibitory role over LiaRS in the absence of stimulus. Together, LiaF and LiaRS form a three-component system characterized by an unusual stoichiometry, a 4:1 ratio between LiaS and LiaR, the significance of which in the signal transduction mechanism of LiaRS is not entirely understood. Read More

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http://dx.doi.org/10.1186/s12866-020-01796-6DOI Listing

Organic mulching positively regulates the soil microbial communities and ecosystem functions in tea plantation.

BMC Microbiol 2020 Apr 29;20(1):103. Epub 2020 Apr 29.

Tea Research Institute, Qingdao Agricultural University, Qingdao, 266109, Shandong, China.

Background: Different mulches have variable effects on soil physicochemical characteristics, bacterial and fungal communities and ecosystem functions. However, the information about soil microbial diversity, community structure and ecosystem function in tea plantation under different mulching patterns was limited. In this study, we investigated bacterial and fungal communities of tea plantation soils under polyethylene film and peanut hull mulching using high-throughput 16S rRNA and ITS rDNA gene Illumina sequencing. Read More

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http://dx.doi.org/10.1186/s12866-020-01794-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7191807PMC
April 2020
2.729 Impact Factor

Extensively drug-resistant Haemophilus influenzae - emergence, epidemiology, risk factors, and regimen.

BMC Microbiol 2020 Apr 28;20(1):102. Epub 2020 Apr 28.

Department of Medical Laboratory Science, College of Medicine, I-Shou University, No.8, Yida Road, Jiaosu Village, Yanchao District, Kaohsiung City, 82445, Taiwan.

Background: Concern about Haemophilus influenzae infection has been increasing over recent decades. Given the emergence of H. influenzae with severe drug resistance, we assessed the prevalence of as well as risk factors and potential therapies for extensively drug-resistant (XDR) H. Read More

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http://dx.doi.org/10.1186/s12866-020-01785-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7189504PMC

Molecular typing of multi-drug resistant Acinetobacter baumannii isolates from clinical and environmental specimens in three Iranian hospitals by pulsed field gel electrophoresis.

BMC Microbiol 2020 Apr 25;20(1):101. Epub 2020 Apr 25.

Department of Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.

Background: Multi-drug resistant (MDR) Acinetobacter baumannii is one of the most important causes of nosocomial infections. The purpose of this study was to identify antibiotic resistance patterns, biofilm formation and the clonal relationship of clinical and environmental isolates of A. baumannii by Pulsed Field Gel Electrophoresis method. Read More

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http://dx.doi.org/10.1186/s12866-020-01792-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7183727PMC
April 2020
2.729 Impact Factor

Multi-omic profiling reveals associations between the gut mucosal microbiome, the metabolome, and host DNA methylation associated gene expression in patients with colorectal cancer.

BMC Microbiol 2020 Apr 23;20(Suppl 1):83. Epub 2020 Apr 23.

State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.

Background: The human gut microbiome plays a critical role in the carcinogenesis of colorectal cancer (CRC). However, a comprehensive analysis of the interaction between the host and microbiome is still lacking.

Results: We found correlations between the change in abundance of microbial taxa, butyrate-related colonic metabolites, and methylation-associated host gene expression in colonic tumour mucosa tissues compared with the adjacent normal mucosa tissues. Read More

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http://dx.doi.org/10.1186/s12866-020-01762-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7178946PMC
April 2020
2.729 Impact Factor

Seasonal variation in gut microbiota composition: cross-sectional evidence from Ukrainian population.

BMC Microbiol 2020 Apr 21;20(1):100. Epub 2020 Apr 21.

Institute of Gerontology, Vyshgorodskaya st. 67, Kyiv, 04114, Ukraine.

Background: Gut microbiota composition is known to depend on environmental (diet, day length, infections, xenobiotic exposure) and lifestyle (alcohol/drug intake, physical activity) factors. All these factors fluctuate seasonally, especially in areas with highly variable climatic conditions between seasons. Seasonal microbiota changes were reported in several previous studies. Read More

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http://dx.doi.org/10.1186/s12866-020-01786-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7175530PMC

Effects of resveratrol on cariogenic virulence properties of Streptococcus mutans.

BMC Microbiol 2020 Apr 17;20(1):99. Epub 2020 Apr 17.

Department of General Dentistry, College of Stomatology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, 639 Zhi Zao Ju Road, Shanghai, 200011, China.

Background: Streptococcus mutans is the principal etiological agent of human dental caries. The major virulence factors of S. mutans are acid production, acid tolerance, extracellular polysaccharide (EPS) synthesis and biofilm formation. Read More

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http://dx.doi.org/10.1186/s12866-020-01761-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7165372PMC

High-throughput sequencing study of the effect of transabdominal hysterectomy on intestinal flora in patients with uterine fibroids.

BMC Microbiol 2020 Apr 15;20(1):98. Epub 2020 Apr 15.

Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical University, Shenyang, 110004, China.

Background: To investigate the effect of transabdominal hysterectomy on the diversity of the intestinal flora in patients with uterine fibroids. Patients with uterine fibroids were selected from September 2018 to December 2018, in the Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical University, and stool specimens were collected from patients before and after surgery.

Results: High-throughput sequencing of the 16S rRNA gene was used to detect the changes in microbial community structure and diversity, and the effects of total hysterectomy on the intestinal flora were further analyzed. Read More

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http://dx.doi.org/10.1186/s12866-020-01779-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7161020PMC

Comparison of methods for the detection of in vitro synergy in multidrug-resistant gram-negative bacteria.

BMC Microbiol 2020 Apr 16;20(1):97. Epub 2020 Apr 16.

Laboratório de Investigação Médica 49 - LIM-49, Instituto de Medicina Tropical, Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo, Avenida Doutor Enéas de Carvalho Aguiar, 470, São Paulo, SP, 05403-000, Brazil.

Background: The use of combined antibiotic therapy has become an option for infections caused by multidrug-resistant (MDR) bacteria. The time-kill (TK) assay is considered the gold standard method for the evaluation of in vitro synergy, but it is a time-consuming and expensive method. The purpose of this study was to evaluate two methods for testing in vitro antimicrobial combinations: the disk diffusion method through disk approximation (DA) and the agar gradient diffusion method via the MIC:MIC ratio. Read More

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http://dx.doi.org/10.1186/s12866-020-01756-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7161189PMC

Design of PCR assays to specifically detect and identify 37 Lactobacillus species in a single 96 well plate.

BMC Microbiol 2020 Apr 15;20(1):96. Epub 2020 Apr 15.

Institute of Life Sciences & Resources and Department of Food Science and Biotechnology, Kyung Hee University, Yongin, 17104, South Korea.

Background: Lactobacillus species are used as probiotics and play an important role in fermented food production. However, use of 16S rRNA gene sequences as standard markers for the differentiation of Lactobacillus species offers a very limited scope, as several species of Lactobacillus share similar 16S rRNA gene sequences. In this study, we developed a rapid and accurate method based on comparative genomic analysis for the identification of 37 Lactobacillus species that are commonly used in probiotics and fermented foods. Read More

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http://dx.doi.org/10.1186/s12866-020-01781-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7160897PMC
April 2020
2.729 Impact Factor

Kobuviruses carried by Rattus norvegicus in Guangdong, China.

BMC Microbiol 2020 Apr 15;20(1):94. Epub 2020 Apr 15.

Department of Epidemiology, School of Public Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, Southern Medical University, 1838 North Road Guangzhou, Guangzhou, 510515, China.

Background: Murine kobuviruses (MuKV) are newly recognized picornaviruses first detected in murine rodents in the USA in 2011. Little information on MuKV epidemiology in murine rodents is available. Therefore, we conducted a survey of the prevalence and genomic characteristics of rat kobuvirus in Guangdong, China. Read More

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http://dx.doi.org/10.1186/s12866-020-01767-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7161169PMC

Difference in virulence between Neisseria meningitidis serogroups W and Y in transgenic mice.

BMC Microbiol 2020 Apr 15;20(1):92. Epub 2020 Apr 15.

Institut Pasteur, Invasive Infections Unit, Paris, France.

Background: Neisseria meningitidis serogroups W and Y are the most common serogroups causing invasive meningococcal disease in Sweden. The majority of cases are caused by the serogroup W UK 2013 strain of clonal complex (cc) 11, and subtype 1 of the serogroup Y, YI strain of cc23. In this study, virulence factors of several lineages within cc11 and cc23 were investigated in transgenic BALB/c mice expressing human transferrin. Read More

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http://dx.doi.org/10.1186/s12866-020-01760-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7160935PMC

Protein-protein interaction of Rv0148 with Htdy and its predicted role towards drug resistance in Mycobacterium tuberculosis.

BMC Microbiol 2020 Apr 15;20(1):93. Epub 2020 Apr 15.

Department of Immunology, ICMR-National Institute for Research in Tuberculosis, #1, Mayor Sathyamoorthy Road, Chetpet, Chennai, 600031, India.

Background: Mycobacterium tuberculosis resides inside host macrophages during infection and adapts to resilient stresses generated by the host immune system. As a response, M. tuberculosis codes for short-chain dehydrogenases/reductases (SDRs). Read More

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http://dx.doi.org/10.1186/s12866-020-01763-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7161113PMC

Silver diamine fluoride (SDF) used in childhood caries management has potent antifungal activity against oral Candida species.

BMC Microbiol 2020 Apr 15;20(1):95. Epub 2020 Apr 15.

Department of Preventive and Restorative Dentistry, University City- Sharjah, University of Sharjah, 27272, United Arab Emirates.

Background: The microbiome of Severe-Early Childhood Caries (S-ECC), is characterized by an ecosystem comprising bacterial and fungal species, with a predominance of Candida species. Hence, an anti-cariogen effective against both bacteria and fungi would be valuable in the management of S-ECC. Here we evaluate the antifungal effect of silver diamine fluoride (SDF) against 35-clinical yeast isolates (Ten-each of C. Read More

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http://dx.doi.org/10.1186/s12866-020-01776-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7161255PMC

Identification of a virulence tal gene in the cotton pathogen, Xanthomonas citri pv. malvacearum strain Xss-V-18.

BMC Microbiol 2020 Apr 15;20(1):91. Epub 2020 Apr 15.

School of Agriculture and Biology, Shanghai Jiao Tong University/Key Laboratory of Urban Agriculture by the Ministry of Agriculture, Shanghai, 200240, China.

Background: Bacterial blight of cotton (BBC), which is caused by the bacterium Xanthomonas citri pv. malvacearum (Xcm), is a destructive disease in cotton. Transcription activator-like effectors (TALEs), encoded by tal-genes, play critical roles in the pathogenesis of xanthomonads. Read More

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http://dx.doi.org/10.1186/s12866-020-01783-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7160923PMC

Validation of in-house liquid direct agglutination test antigen: the potential diagnostic test in visceral Leishimaniasis endemic areas of Northwest Ethiopia.

BMC Microbiol 2020 Apr 15;20(1):90. Epub 2020 Apr 15.

Armauer Hansen Research Institute, Addis Ababa, Ethiopia.

Background: Visceral leishmaniasis in Ethiopia is a re-emerging threat to public health, with increased geographical distribution and number of cases. It is a fatal disease without early diagnosis and treatment; thus, the availability of affordable diagnostic tools is crucial. However, due to delays caused by import regulations, procurement and late delivery of imported test kits, accessibility remains a problem in the control program. Read More

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http://dx.doi.org/10.1186/s12866-020-01780-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7158028PMC
April 2020
2.729 Impact Factor

Genetic and virulence characterisation of Vibrio parahaemolyticus isolated from Indian coast.

BMC Microbiol 2020 Mar 23;20(1):62. Epub 2020 Mar 23.

Cholera and Biofilm Research Lab, Department of Pathogen Biology, Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram, Kerala, 695 014, India.

Background: V. parahaemolyticus is autochthonous to the marine environment and causes seafood-borne gastroenteritis in humans. Generally, V. Read More

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http://dx.doi.org/10.1186/s12866-020-01746-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7092547PMC

Value of multiplex PCR for detection of antimicrobial resistance in samples retrieved from patients with orthopaedic infections.

BMC Microbiol 2020 Apr 14;20(1):88. Epub 2020 Apr 14.

Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Center for Musculoskeletal Surgery (CMSC), Charitéplatz 1, 10117, Berlin, Germany.

Background: The performance of multiplex PCR (mPCR) for detection of antimicrobial resistance from clinical isolates is unknown. We assessed the ability of mPCR to analyse resistance genes directly from clinical samples. Patients with orthopedic infections were prospectively included. Read More

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http://dx.doi.org/10.1186/s12866-020-01741-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7155317PMC