Transcriptomic data from two primary cell models stimulating human monocytes suggest inhibition of oxidative phosphorylation and mitochondrial function by N. meningitidis which is partially up-regulated by IL-10.
- Unni Gopinathan,
- Reidun Øvstebø,
- Berit Sletbakk Brusletto,
- Ole Kristoffer Olstad,
- Peter Kierulf,
- Petter Brandtzaeg,
- Jens Petter Berg
BMC Immunol 2017 Oct 27;18(1):46. Epub 2017 Oct 27.
Blood Cell Research Group, Section for Research, Department of Medical Biochemistry, Oslo University Hospital, Oslo, Norway.
Background: Biological interpretation of DNA microarray data may differ depending on underlying assumptions and statistical tests of bioinformatics tools used. We used Gene Set Enrichment Analysis (GSEA) and Ingenuity Pathway Analysis (IPA) to analyze previously generated DNA microarray data from human monocytes stimulated with N. meningitidis and IL-10 ("the model system"), and with meningococcal sepsis plasma before and after immunodepletion of IL-10 ("the patient plasma system"). Read More