- Matteo Chiara,
- Antonio Placido,
- Ernesto Picardi,
- Luigi Ruggiero Ceci,
- David Stephen Horner,
- Graziano Pesole
BMC Genomics 2018 Jan 12;19(1):44. Epub 2018 Jan 12.
Institute of Biomembranes, Bioenergetics and Molecular Biotechnology, Consiglio Nazionale delle Ricerche, via Amendola 165A, 70126, Bari, Italy.
Background: Expression screening of environmental DNA (eDNA) libraries is a popular approach for the identification and characterization of novel microbial enzymes with promising biotechnological properties. In such "functional metagenomics" experiments, inserts, selected on the basis of activity assays, are sequenced with high throughput sequencing technologies. Assembly is followed by gene prediction, annotation and identification of candidate genes that are subsequently evaluated for biotechnological applications. Read More