871 results match your criteria BMC Cell Biology [Journal]


Shikonin sensitizes A549 cells to TRAIL-induced apoptosis through the JNK, STAT3 and AKT pathways.

BMC Cell Biol 2018 Dec 29;19(1):29. Epub 2018 Dec 29.

Beijing Key Laboratory of Research of Chinese Medicine on Prevention and Treatment for Major Diseases, Experimental Research Center, China Academy of Chinese Medical Sciences, 16 Dong Zhi Men Nei Street, Dong Cheng District, Beijing, China.

Background: TRAIL, tumor necrosis factor-related apoptosis-inducing ligand, can selectively kill cancer cells with little or no cytotoxicity toward normal human cells and is regarded as a potential relatively safe antitumor drug. However, some cancer cells are resistant to TRAIL-induced apoptosis. Thus, reagents that potentiate TRAIL-induced cytotoxicity are needed. Read More

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http://dx.doi.org/10.1186/s12860-018-0179-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6310954PMC
December 2018
10 Reads

Mitotic activity patterns and cytoskeletal changes throughout the progression of diapause developmental program in Daphnia.

BMC Cell Biol 2018 Dec 29;19(1):30. Epub 2018 Dec 29.

Department of Animal Ecology, Evolution and Biodiversity, Ruhr-University Bochum, NDEF 05/751, Universitaetsstrasse 150, 44780, Bochum, Germany.

Background: Diapause is a form of dormancy that is genetically predetermined to allow animals to overcome harsh environmental conditions. It is induced by predictive environmental cues bringing cellular activity levels into a state of suspended animation. Entering diapause requires organismal, molecular and cellular adaptation to severely reduced energy flows. Read More

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http://dx.doi.org/10.1186/s12860-018-0181-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6310958PMC
December 2018
2 Reads

Post-treatment de-phosphorylation of p53 correlates with dasatinib responsiveness in malignant melanoma.

BMC Cell Biol 2018 Dec 27;19(1):28. Epub 2018 Dec 27.

Department of Physiology & Medical Physics, Royal College of Surgeons in Ireland, Dublin 2, Ireland.

Background: Dasatinib (Sprycel) was developed as a tyrosine kinase inhibitor targeting Bcr-Abl and the family of Src kinases. Dasatinib is commonly used for the treatment of acute lymphoblastic and chronic myelogenous leukemia. Previous clinical studies in melanoma returned inconclusive results and suggested that patients respond highly heterogeneously to dasatinib as single agent or in combination with standard-of-care chemotherapeutic dacarbazine. Read More

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http://dx.doi.org/10.1186/s12860-018-0180-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6307246PMC
December 2018
10 Reads

Comparative evaluation of mesenchymal stromal cells from umbilical cord and amniotic membrane in xeno-free conditions.

BMC Cell Biol 2018 Dec 13;19(1):27. Epub 2018 Dec 13.

Department of Rheumatology, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi Pvovince, China.

Background: Within the past years, umbilical cord (UC) and amniotic membrane (AM) expanded in human platelet lysate (PL) have been found to become increasingly candidate of mesenchymal stromal cells (MSCs) in preclinical and clinical studies. Different sources of MSCs have different properties, and lead to different therapeutic applications. However, the similarity and differences between the AMMSCs and UCMSCs in PL remain unclear. Read More

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http://dx.doi.org/10.1186/s12860-018-0178-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6293527PMC
December 2018
1 Read

The STRIPAK complex components FAM40A and FAM40B regulate endothelial cell contractility via ROCKs.

BMC Cell Biol 2018 Dec 3;19(1):26. Epub 2018 Dec 3.

Randall Centre for Cell and Molecular Biophysics, King's College London, New Hunt's House, Guy's Campus, London, SE1 1UL, UK.

Background: Endothelial cells provide a barrier between blood and tissues, which is regulated to allow molecules and cells in out of tissues. Patients with cerebral cavernous malformations (CCM) have dilated leaky blood vessels, especially in the central nervous system. A subset of these patients has loss-of-function mutations in CCM3. Read More

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http://dx.doi.org/10.1186/s12860-018-0175-yDOI Listing
December 2018
2 Reads

Kif17 phosphorylation regulates photoreceptor outer segment turnover.

BMC Cell Biol 2018 Nov 20;19(1):25. Epub 2018 Nov 20.

Department of Cell Biology, Neurobiology, and Anatomy Medical College of Wisconsin, Milwaukee, WI, USA.

Background: KIF17, a kinesin-2 motor that functions in intraflagellar transport, can regulate the onset of photoreceptor outer segment development. However, the function of KIF17 in a mature photoreceptor remains unclear. Additionally, the ciliary localization of KIF17 is regulated by a C-terminal consensus sequence (KRKK) that is immediately adjacent to a conserved residue (mouse S1029/zebrafish S815) previously shown to be phosphorylated by CaMKII. Read More

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http://dx.doi.org/10.1186/s12860-018-0177-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6245759PMC
November 2018
8 Reads

PIAS1 protects against myocardial ischemia-reperfusion injury by stimulating PPARγ SUMOylation.

BMC Cell Biol 2018 Nov 12;19(1):24. Epub 2018 Nov 12.

Department of Cardiovascular Surgery, Renji Hospital, Shanghai Jiao Tong University School of Medicine, 160 Pujian Road, Shanghai, 200127, China.

Background: Myocardial ischemia-reperfusion injury (IRI) has become one of the most serious complications after reperfusion therapy in patients with acute myocardial infarction. Small ubiquitin-like modification (SUMOylation) is a reversible process, including SUMO E1-, E2-, and E3-mediated SUMOylation and SUMO-specific protease-mediated deSUMOylation, with the latter having been shown to play a vital role in myocardial IRI previously. However, little is known about the function and regulation of SUMO E3 ligases in myocardial IRI. Read More

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https://bmccellbiol.biomedcentral.com/articles/10.1186/s1286
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http://dx.doi.org/10.1186/s12860-018-0176-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6233564PMC
November 2018
10 Reads

Acetylation of C-terminal lysines modulates protein turnover and stability of Connexin-32.

BMC Cell Biol 2018 09 29;19(1):22. Epub 2018 Sep 29.

Department of Neurology & Rehabilitation, University of Illinois at Chicago, Chicago, IL, 60612, USA.

Background: The gap junction protein, Connexin32 (Cx32), is expressed in various tissues including liver, exocrine pancreas, gastrointestinal epithelium, and the glia of the central and peripheral nervous system. Gap junction-mediated cell-cell communication and channel-independent processes of Cx32 contribute to the regulation of physiological and cellular activities such as glial differentiation, survival, and proliferation; maintenance of the hepatic epithelium; and axonal myelination. Mutations in Cx32 cause X-linked Charcot-Marie-Tooth disease (CMT1X), an inherited peripheral neuropathy. Read More

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http://dx.doi.org/10.1186/s12860-018-0173-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6162937PMC
September 2018
7 Reads

Inflammation caused by peripheral immune cells across into injured mouse blood brain barrier can worsen postoperative cognitive dysfunction induced by isoflurane.

BMC Cell Biol 2018 09 29;19(1):23. Epub 2018 Sep 29.

Department of Anesthesiology, Zhongshan Hospital, Fudan University, No. 180 Fenglin Road, Xuhui District, Shanghai, 200032, People's Republic of China.

Background: Disruption to the blood brain barrier (BBB) is a leading factor associated with the development of postoperative cognitive dysfunction (POCD). Despite this, the underlying mechanism by which BBB disruption promotes POCD in the elderly population has not yet been not fully elucidated.

Results: In this study, we established a POCD mice model using isoflurane, and observed the highly expressed occludin and claudin 5 in brain tissues concomitant with the increased enrichment of CD4 positive cells and NK cells in the hippocampus of POCD mice compared to normal and non-POCD control. Read More

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http://dx.doi.org/10.1186/s12860-018-0172-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6162931PMC
September 2018
2 Reads

Tanshinone IIA reduces SW837 colorectal cancer cell viability via the promotion of mitochondrial fission by activating JNK-Mff signaling pathways.

BMC Cell Biol 2018 09 25;19(1):21. Epub 2018 Sep 25.

Department of Traditional Chinese Medicine, Central South University Third Xiangya Hospital, Changsha, 410013, China.

Background: Mitochondrial homeostasis has been increasingly viewed as a potential target of cancer therapy, and mitochondrial fission is a novel regulator of mitochondrial function and apoptosis. The aim of our study was to determine the detailed role of mitochondrial fission in SW837 colorectal cancer cell viability, mobility and proliferation. In addition, the current study also investigated the therapeutic impact of Tanshinone IIA (Tan IIA), a type of anticancer adjuvant drug, on cancer mitochondrial homeostasis. Read More

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https://bmccellbiol.biomedcentral.com/articles/10.1186/s1286
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http://dx.doi.org/10.1186/s12860-018-0174-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6157045PMC
September 2018
8 Reads

Correction to: The M-phase specific hyperphosphorylation of Staufen2 involved the cyclin-dependent kinase CDK1.

BMC Cell Biol 2018 09 10;19(1):20. Epub 2018 Sep 10.

Département de biochimie et médecine moléculaire, Faculté de médecine, Université de Montréal, 2900 Edouard Montpetit, Montréal, QC, H3T 1J4, Canada.

Following publication of the original article [1], the authors reported a change to one of the author names. Read More

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http://dx.doi.org/10.1186/s12860-018-0171-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6130059PMC
September 2018
4 Reads

Treatment of Trypanosoma cruzi with 2-bromopalmitate alters morphology, endocytosis, differentiation and infectivity.

BMC Cell Biol 2018 08 31;19(1):19. Epub 2018 Aug 31.

Laboratory of Cell Biology, Carlos Chagas Institute/Fiocruz-PR, 81310-020 Curitiba, Paraná, Brazil.

Background: The palmitate analogue 2-bromopalmitate (2-BP) is a non-selective membrane tethered cysteine alkylator of many membrane-associated enzymes that in the last years emerged as a general inhibitor of protein S-palmitoylation. Palmitoylation is a post-translational protein modification that adds palmitic acid to a cysteine residue through a thioester linkage, promoting membrane localization, protein stability, regulation of enzymatic activity, and the epigenetic regulation of gene expression. Little is known on such important process in the pathogenic protozoan Trypanosoma cruzi, the etiological agent of Chagas disease. Read More

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http://dx.doi.org/10.1186/s12860-018-0170-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6119340PMC
August 2018
7 Reads

Lipopolysaccharide induces bacterial autophagy in epithelial keratinocytes of the gingival sulcus.

BMC Cell Biol 2018 08 30;19(1):18. Epub 2018 Aug 30.

Research Center for Regenerative Medicine, Fukuoka Dental College, Fukuoka, Japan.

Background: Interactions of resident bacteria and/or their producing lipopolysaccharide (LPS) with sulcular epithelial keratinocytes may be regulated by autophagy in the gingival sulcus. In this study, we investigated an induction of bacterial autophagy in exfoliative sulcular keratinocytes of the gingival sulcus and cultured keratinocytes treated with Porphyromonas gingivalis-originated LPS (PgLPS).

Results: Exfoliative sulcular keratinocytes showed an induction of autophagy, in addition to increased expression of LPS-mediated factors including lipopolysaccharide-binding protein and toll-like receptors (TLRs), leading to co-localization of bacteria with autophagosomes. Read More

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http://dx.doi.org/10.1186/s12860-018-0168-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6117973PMC
August 2018
1 Read

Fibroblasts from bank voles inhabiting Chernobyl have increased resistance against oxidative and DNA stresses.

BMC Cell Biol 2018 08 29;19(1):17. Epub 2018 Aug 29.

Department of Ecology and Genetics, University of Oulu, FI-90014, Oulu, Finland.

Background: Elevated levels of environmental ionizing radiation can be a selective pressure for wildlife by producing reactive oxygen species and DNA damage. However, the underlying molecular mechanisms that are affected are not known.

Results: We isolated skin fibroblasts from bank voles (Myodes glareolus) inhabiting the Chernobyl nuclear power plant accident site where background radiation levels are about 100 times greater than in uncontaminated areas. Read More

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https://bmccellbiol.biomedcentral.com/articles/10.1186/s1286
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http://dx.doi.org/10.1186/s12860-018-0169-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6114495PMC
August 2018
14 Reads

Mutant TP53 G245C and R273H promote cellular malignancy in esophageal squamous cell carcinoma.

BMC Cell Biol 2018 08 20;19(1):16. Epub 2018 Aug 20.

State Key Laboratory of Molecular Oncology, Chinese Academy of Medical Sciences and Peking Union Medical College, National Cancer Center/Cancer Hospital, Beijing, 100021, China.

Background: TP53 gene mutations occur in more than 50% of human cancers and the vast majority of these mutations in human cancers are missense mutations, which broadly occur in DNA binding domain (DBD) (Amino acids 102-292) and mainly reside in six "hotspot" residues. TP53 G245C and R273H point mutations are two of the most frequent mutations in tumors and have been verified in several different cancers. In the previous study of the whole genome sequencing (WGS), we found some mutations of TP53 DBD in esophageal squamous cell carcinoma (ESCC) clinical samples. Read More

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http://dx.doi.org/10.1186/s12860-018-0167-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6102840PMC
August 2018
55 Reads

Generation of renewable mouse intestinal epithelial cell monolayers and organoids for functional analyses.

BMC Cell Biol 2018 08 15;19(1):15. Epub 2018 Aug 15.

Department of Cell Biology and Physiology, University of North Carolina at Chapel Hill, 111 Mason Farm Road, 6340C MBRB, CB #7545, Chapel Hill, NC, 27599-7545, USA.

Background: Conditional reprogramming has enabled the development of long-lived, normal epithelial cell lines from mice and humans by in vitro culture with ROCK inhibitor on a feeder layer. We applied this technology to mouse small intestine to create 2D mouse intestinal epithelial monolayers (IEC monolayers) from genetic mouse models for functional analysis.

Results: IEC monolayers form epithelial colonies that proliferate on a feeder cell layer and are able to maintain their genotype over long-term passage. Read More

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https://bmccellbiol.biomedcentral.com/articles/10.1186/s1286
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http://dx.doi.org/10.1186/s12860-018-0165-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6094565PMC
August 2018
24 Reads

Novel roles for scleraxis in regulating adult tenocyte function.

BMC Cell Biol 2018 08 7;19(1):14. Epub 2018 Aug 7.

Department of Large Animal Clinical Sciences, Virginia-Maryland College of Veterinary Medicine, Virginia Tech, 205 Duck Pond Drive, Blacksburg, VA, 24061-0442, USA.

Background: Tendinopathies are common and difficult to resolve due to the formation of scar tissue that reduces the mechanical integrity of the tissue, leading to frequent reinjury. Tenocytes respond to both excessive loading and unloading by producing pro-inflammatory mediators, suggesting that these cells are actively involved in the development of tendon degeneration. The transcription factor scleraxis (Scx) is required for the development of force-transmitting tendon during development and for mechanically stimulated tenogenesis of stem cells, but its function in adult tenocytes is less well-defined. Read More

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https://bmccellbiol.biomedcentral.com/articles/10.1186/s1286
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http://dx.doi.org/10.1186/s12860-018-0166-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6081934PMC
August 2018
7 Reads

Cyclic AMP signaling in Dictyostelium promotes the translocation of the copine family of calcium-binding proteins to the plasma membrane.

BMC Cell Biol 2018 07 16;19(1):13. Epub 2018 Jul 16.

Biology Department, Central Michigan University, Mount Pleasant, MI, 48859, USA.

Background: Copines are calcium-dependent phospholipid-binding proteins found in many eukaryotic organisms and are thought to be involved in signaling pathways that regulate a wide variety of cellular processes. Copines are characterized by having two C2 domains at the N-terminus accompanied by an A domain at the C-terminus. Six copine genes have been identified in the Dictyostelium genome, cpnA - cpnF. Read More

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http://dx.doi.org/10.1186/s12860-018-0160-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6048903PMC
July 2018
4 Reads

mTOR and ROS regulation by anethole on adipogenic differentiation in human mesenchymal stem cells.

BMC Cell Biol 2018 07 6;19(1):12. Epub 2018 Jul 6.

Beckman Laser Institute Korea, Dankook University, 119 Dandae-ro, Cheonan, 31116, Republic of Korea.

Background: Adipocyte differentiation of human mesenchymal stem cells (hMSCs) is dependent on mitochondrial metabolism and reactive oxygen species (ROS) to initiate adipocyte differentiation. Although anethole has been known as an anti-oxidant and lipid peroxidation inhibitor, there is little investigated about its role in adipogenic differentiation.

Methods: The effects on cytotoxicity and proliferation of anethole in hMSCs were measured by the MTT assay. Read More

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http://dx.doi.org/10.1186/s12860-018-0163-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6035441PMC
July 2018
11 Reads

Caspase-independent cell death does not elicit a proliferative response in melanoma cancer cells.

BMC Cell Biol 2018 07 4;19(1):11. Epub 2018 Jul 4.

INSERM 1052, CNRS 5286, Cancer Research Center of Lyon (CRCL), Lyon, France.

Background: Apoptosis, the most well-known type of programmed cell death, can induce in a paracrine manner a proliferative response in neighboring surviving cells called apoptosis-induced proliferation (AiP). While having obvious benefits when triggered in developmental processes, AiP is a serious obstacle in cancer therapy, where apoptosis is frequently induced by chemotherapy. Therefore, in this study, we evaluated the capacity of an alternative type of cell death, called caspase-independent cell death, to promote proliferation. Read More

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http://dx.doi.org/10.1186/s12860-018-0164-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6030751PMC
July 2018
3 Reads

Establishment and comparison of air-liquid interface culture systems for primary and immortalized swine tracheal epithelial cells.

BMC Cell Biol 2018 06 28;19(1):10. Epub 2018 Jun 28.

Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, 50 Zhongling Street, Nanjing, 210014, China.

Background: Air-liquid interface (Ali) systems allow the establishment of a culture environment more representative of that in vivo than other culture systems. They are useful for performing mechanistic studies of respiratory epithelial cells as drug permeation barriers and can be used to study the interactions between hosts and respiratory pathogens. However, there have been few studies concerning Ali cultures of primary swine tracheal epithelial cells (STECs) and an immortalized STEC line, and the differences between these two systems remain poorly defined. Read More

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http://dx.doi.org/10.1186/s12860-018-0162-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6025731PMC
June 2018
28 Reads
2.341 Impact Factor

Mannan oligosaccharide requires functional ETC and TLR for biological radiation protection to normal cells.

BMC Cell Biol 2018 06 27;19(1). Epub 2018 Jun 27.

Division of Capacity Enhancement and Product Induction, Institute of Nuclear Medicine & Allied Sciences, Defence Research and Development Organization, Ministry of Defence, Brig. S.K. Mazumdar Marg, Timarpur, Delhi, 110054, India.

Background: Low LET Ionizing radiation is known to alter intracellular redox balance by inducing free radical generation, which may cause oxidative modification of various cellular biomolecules. The extent of biomolecule-modifications/ damages and changes in vital processes (viz. cellular homeostasis, inter-/intra-cellular signaling, mitochondrial physiology/dynamics antioxidant defence systems) are crucial which in turn determine fate of cells. Read More

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http://dx.doi.org/10.1186/s12860-018-0161-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6020349PMC
June 2018
4 Reads

An easy-to-build and re-usable microfluidic system for live-cell imaging.

BMC Cell Biol 2018 06 20;19(1). Epub 2018 Jun 20.

SyntheCell team, Institute of Genetics and Development of Rennes, CNRS UMR 6290, 2 avenue du Pr. Léon Bernard, 35043, Rennes, France.

Background: Real-time monitoring of cellular responses to dynamic changes in their environment or to specific treatments has become central to cell biology. However, when coupled to live-cell imaging, such strategies are difficult to implement with precision and high time resolution, and the simultaneous alteration of multiple parameters is a major challenge. Recently, microfluidics has provided powerful solutions for such analyses, bringing an unprecedented level of control over the conditions and the medium in which cells under microscopic observation are grown. Read More

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http://dx.doi.org/10.1186/s12860-018-0158-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6011407PMC
June 2018
4 Reads

A comparison of LKB1/AMPK/mTOR metabolic axis response to global ischaemia in brain, heart, liver and kidney in a rat model of cardiac arrest.

BMC Cell Biol 2018 06 19;19(1). Epub 2018 Jun 19.

Centre for Neuroscience, Neuronal Injury and Repair Laboratory, College of Medicine and Public Health, Flinders University of South Australia, Adelaide, SA, 5042, Australia.

Background: Cellular energy failure in high metabolic rate organs is one of the underlying causes for many disorders such as neurodegenerative diseases, cardiomyopathies, liver and renal failures. In the past decade, numerous studies have discovered the cellular axis of LKB1/AMPK/mTOR as an essential modulator of cell homeostasis in response to energy stress. Through regulating adaptive mechanisms, this axis adjusts the energy availability to its demand by a systematized control on metabolism. Read More

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http://dx.doi.org/10.1186/s12860-018-0159-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6010165PMC
June 2018
6 Reads

The C-terminus of the oncoprotein TGAT is necessary for plasma membrane association and efficient RhoA-mediated signaling.

BMC Cell Biol 2018 06 7;19(1). Epub 2018 Jun 7.

Swammerdam Institute for Life Sciences, Section of Molecular Cytology, van Leeuwenhoek Centre for Advanced Microscopy, University of Amsterdam, P.O. Box 94215, NL, -1090, GE, Amsterdam, The Netherlands.

Background: Rho guanine exchange factors (RhoGEFs) control cellular processes such as migration, adhesion and proliferation. Alternative splicing of the RhoGEF Trio produces TGAT. The RhoGEF TGAT is an oncoprotein with constitutive RhoGEF activity. Read More

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http://dx.doi.org/10.1186/s12860-018-0155-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5992656PMC
June 2018
3 Reads

Overexpression of Contactin 1 promotes growth, migration and invasion in Hs578T breast cancer cells.

BMC Cell Biol 2018 04 19;19(1). Epub 2018 Apr 19.

Department of Surgical Oncology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, China.

Background: Contactin1 (CNTN1) has been shown to play an important role in the invasion and metastasis of several tumors; however, the role of CNTN1 in breast cancer has not been fully studied. The purpose of this study is to investigate the role of CNTN1 in regulating tumor growth, migration and invasion in breast cancer.

Results: To investigate its function, CNTN1 was expressed in Hs578T cells. Read More

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http://dx.doi.org/10.1186/s12860-018-0154-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5907708PMC
April 2018
15 Reads

Foreskin-derived mesenchymal stromal cells with aldehyde dehydrogenase activity: isolation and gene profiling.

BMC Cell Biol 2018 04 6;19(1). Epub 2018 Apr 6.

Laboratory of Clinical Cell Therapy, Jules Bordet Institute, Université Libre de Bruxelles (ULB), Campus Erasme, Bâtiment de Transfusion (Level +1), Route de Lennik 808, 1070, Brussels, Belgium.

Background: Mesenchymal stromal cells (MSCs) become an attractive research topic because of their crucial roles in tissue repair and regenerative medicine. Foreskin is considered as a valuable tissue source containing immunotherapeutic MSCs (FSK-MSCs).

Results: In this work, we used aldehyde dehydrogenase activity (ALDH) assay (ALDEFLUOR™) to isolate and therefore characterize subsets of FSK-MSCs. Read More

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http://dx.doi.org/10.1186/s12860-018-0157-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5889569PMC
April 2018
6 Reads

Role of the HIV-1 envelope transmembrane domain in intracellular sorting.

BMC Cell Biol 2018 03 15;19(1). Epub 2018 Mar 15.

Department of Cell Physiology and Metabolism, Faculty of Medicine, University of Geneva, 1 rue Michel Servet, 1211, Geneva 4, Switzerland.

Background: The envelope protein of lentiviruses are type I transmembrane proteins, and their transmembrane domain contains conserved potentially charged residues. This highly unusual feature would be expected to cause endoplasmic reticulum (ER) localization. The aim of this study was to determine by which means the HIV-1 Env protein is transported to the cell surface although its transmembrane domain contains a conserved arginine residue. Read More

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http://dx.doi.org/10.1186/s12860-018-0153-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5856207PMC
March 2018
4 Reads

Separation of functionally divergent muscle precursor cell populations from porcine juvenile muscles by discontinuous Percoll density gradient centrifugation.

BMC Cell Biol 2018 03 9;19(1). Epub 2018 Mar 9.

Leibniz Institute for Farm Animal Biology (FBN), Institute of Muscle Biology and Growth, Growth and Development Unit, Wilhelm-Stahl-Allee 2, 18196, Dummerstorf, Germany.

Background: Satellite cells (SC) and their descendants, muscle precursor cells (MPC), play a key role in postnatal muscle development, regeneration, and plasticity. Several studies have provided evidence that SC and MPC represent a heterogeneous population differing in their biochemical and functional properties. The identification and characterization of functionally divergent SC subpopulations should help to reveal the precise involvement of SC/MPC in these myogenic processes. Read More

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http://dx.doi.org/10.1186/s12860-018-0156-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5845299PMC
March 2018
10 Reads

MicroRNA-17-5p aggravates lipopolysaccharide-induced injury in nasal epithelial cells by targeting Smad7.

BMC Cell Biol 2018 02 13;19(1). Epub 2018 Feb 13.

Department of Allergy, Wuhan No.1 Hospital, No. 215, Zhongshan Avenue, Wuhan, 430022, China.

Background: Globally, rhinitis is one of the most common chronic disorders. Despite availability of drugs to manage the symptomatology of rhinitis, researchers still focus on identification of novel molecular targets for better management. MicroRNAs are implicated in many biological and pathological processes. Read More

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http://dx.doi.org/10.1186/s12860-018-0152-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5809994PMC
February 2018
6 Reads

Mechanically tuned 3 dimensional hydrogels support human mammary fibroblast growth and viability.

BMC Cell Biol 2017 Dec 16;18(1):35. Epub 2017 Dec 16.

Department of Biomedical Sciences, Georgia Philadelphia College of Osteopathic Medicine, Suwanee, GA, 30024, USA.

Background: Carcinoma associated fibroblasts (CAFs or myofibroblasts) are activated fibroblasts which participate in breast tumor growth, angiogenesis, invasion, metastasis and therapy resistance. As such, recent efforts have been directed toward understanding the factors responsible for activation of the phenotype. In this study, we have investigated how changes in the mechanical stiffness of a 3D hydrogel alter the behavior and myofibroblast-like properties of human mammary fibroblasts (HMFs). Read More

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http://dx.doi.org/10.1186/s12860-017-0151-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5732527PMC
December 2017
8 Reads

Molecular targets and signaling pathways regulated by nuclear translocation of syndecan-1.

BMC Cell Biol 2017 Dec 8;18(1):34. Epub 2017 Dec 8.

Department of Laboratory Medicine, Division of Pathology, Karolinska Institutet, SE-14186, Stockholm, Sweden.

Background: The cell-surface heparan sulfate proteoglycan syndecan-1 is important for tumor cell proliferation, migration, and cell cycle regulation in a broad spectrum of malignancies. Syndecan-1, however, also translocates to the cell nucleus, where it might regulate various molecular functions.

Results: We used a fibrosarcoma model to dissect the functions of syndecan-1 related to the nucleus and separate them from functions related to the cell-surface. Read More

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http://dx.doi.org/10.1186/s12860-017-0150-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5721467PMC
December 2017
6 Reads

A novel interaction between kinase activities in regulation of cilia formation.

BMC Cell Biol 2017 Nov 15;18(1):33. Epub 2017 Nov 15.

Anatomy and Cell Biology, East Carolina University, Brody School of Medicine, Greenville, NC, USA.

Background: The primary cilium is an extension of the cell membrane that encloses a microtubule-based axoneme. Primary cilia are essential for transmission of environmental cues that determine cell fate. Disruption of primary cilia function is the molecular basis of numerous developmental disorders. Read More

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http://dx.doi.org/10.1186/s12860-017-0149-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5688660PMC
November 2017
9 Reads

Reduction of pluripotent gene expression in murine embryonic stem cells exposed to mechanical loading or Cyclo RGD peptide.

BMC Cell Biol 2017 Nov 14;18(1):32. Epub 2017 Nov 14.

McCaig Institute for Bone and Joint Health, University of Calgary, Calgary, AB, Canada.

Background: Self-renewal and differentiation of embryonic stem cells (ESCs) is directed by biological and/or physical cues that regulate multiple signaling cascades. We have previously shown that mESCs seeded in a type I collagen matrix demonstrate a loss of pluripotent marker expression and differentiate towards an osteogenic lineage. In this study, we examined if this effect was mediated in part through Arginylglycylaspartic acid (RGD) dependent integrin activity and/or mechano-transduction. Read More

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http://dx.doi.org/10.1186/s12860-017-0148-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5686896PMC
November 2017
8 Reads

Small molecule modulation of splicing factor expression is associated with rescue from cellular senescence.

BMC Cell Biol 2017 10 17;18(1):31. Epub 2017 Oct 17.

Institute of Biomedical and Clinical Sciences, University of Exeter Medical School, University of Exeter, Barrack Road, Exeter, Devon, EX2 5DW, UK.

Background: Altered expression of mRNA splicing factors occurs with ageing in vivo and is thought to be an ageing mechanism. The accumulation of senescent cells also occurs in vivo with advancing age and causes much degenerative age-related pathology. However, the relationship between these two processes is opaque. Read More

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http://dx.doi.org/10.1186/s12860-017-0147-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5645932PMC
October 2017
16 Reads

Honokiol improved chondrogenesis and suppressed inflammation in human umbilical cord derived mesenchymal stem cells via blocking nuclear factor-κB pathway.

BMC Cell Biol 2017 08 29;18(1):29. Epub 2017 Aug 29.

Department of Orthopaedics, The First Affiliated Hospital, College of Medicine, Xi'an Jiaotong University, Xi'an, 710061, People's Republic of China.

Background: Cartilage degradation is the significant pathological process in osteoarthritis (OA). Inflammatory cytokines, such as interleukin-1β (IL-1β), activate various downstream mediators contributing to OA pathology. Recently, stem cell-based cartilage repair emerges as a potential therapeutic strategy that being widely studied, whereas, the outcome is still far from clinical application. Read More

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http://dx.doi.org/10.1186/s12860-017-0145-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5576244PMC
August 2017
18 Reads

Coral cell separation and isolation by fluorescence-activated cell sorting (FACS).

BMC Cell Biol 2017 08 29;18(1):30. Epub 2017 Aug 29.

University of Miami, Rosenstiel School of Marine and Atmospheric Science, 4600 Rickenbacker Causeway, Florida, 33149, USA.

Background: Generalized methods for understanding the cell biology of non-model species are quite rare, yet very much needed. In order to address this issue, we have modified a technique traditionally used in the biomedical field for ecological and evolutionary research. Fluorescent activated cell sorting (FACS) is often used for sorting and identifying cell populations. Read More

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http://dx.doi.org/10.1186/s12860-017-0146-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5575905PMC
August 2017
10 Reads

EphA receptors and ephrin-A ligands are upregulated by monocytic differentiation/maturation and promote cell adhesion and protrusion formation in HL60 monocytes.

BMC Cell Biol 2017 08 29;18(1):28. Epub 2017 Aug 29.

Laboratory of Veterinary Anatomy, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, 1-58 Rinku-Ourai-Kita, Izumisano, Osaka, 598-8531, Japan.

Background: Eph signaling is known to induce contrasting cell behaviors such as promoting and inhibiting cell adhesion/spreading by altering F-actin organization and influencing integrin activities. We have previously demonstrated that EphA2 stimulation by ephrin-A1 promotes cell adhesion through interaction with integrins and integrin ligands in two monocyte/macrophage cell lines. Although mature mononuclear leukocytes express several members of the EphA/ephrin-A subclass, their expression has not been examined in monocytes undergoing during differentiation and maturation. Read More

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http://bmccellbiol.biomedcentral.com/articles/10.1186/s12860
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http://dx.doi.org/10.1186/s12860-017-0144-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5576293PMC
August 2017
8 Reads

Cladosporol A triggers apoptosis sensitivity by ROS-mediated autophagic flux in human breast cancer cells.

BMC Cell Biol 2017 07 20;18(1):26. Epub 2017 Jul 20.

Cancer Pharmacology Division, CSIR-Indian Institute of Integrative Medicine, Jammu, India.

Background: Endophytes have proven to be an invaluable resource of chemically diverse secondary metabolites that act as excellent lead compounds for anticancer drug discovery. Here we report the promising cytotoxic effects of Cladosporol A (HPLC purified >98%) isolated from endophytic fungus Cladosporium cladosporioides collected from Datura innoxia. Cladosporol A was subjected to in vitro cytotoxicity assay against NCI60 panel of human cancer cells using MTT assay. Read More

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http://dx.doi.org/10.1186/s12860-017-0141-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5520384PMC
July 2017
49 Reads

The M-phase specific hyperphosphorylation of Staufen2 involved the cyclin-dependent kinase CDK1.

BMC Cell Biol 2017 07 14;18(1):25. Epub 2017 Jul 14.

Département de biochimie et médecine moléculaire, Faculté de médecine, Université de Montréal, 2900 Edouard Montpetit, Montréal, QC, H3T 1J4, Canada.

Background: Staufen2 (STAU2) is an RNA-binding protein involved in the post-transcriptional regulation of gene expression. This protein was shown to be required for organ formation and cell differentiation. Although STAU2 functions have been reported in neuronal cells, its role in dividing cells remains deeply uncharacterized. Read More

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http://dx.doi.org/10.1186/s12860-017-0142-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5513041PMC
July 2017
15 Reads

External magnetic field promotes homing of magnetized stem cells following subcutaneous injection.

BMC Cell Biol 2017 05 26;18(1):24. Epub 2017 May 26.

Department of Nuclear Medicine, the First Hospital Affiliated to Jinan University, No. 613 Huangpu West Road, Guangzhou, 510630, China.

Background: Mesenchymal stem cells (MSCs) are multipotent stromal cells that have the ability to self-renew and migrate to sites of pathology. In vivo tracking of MSCs provides insights into both, the underlying mechanisms of MSC transformation and their potential as gene delivery vehicles. The aim of our study was to assess the ability of superparamagnetic iron oxide nanoparticles (SPIONs)-labeled Wharton's Jelly of the human umbilical cord-derived MSCs (WJ-MSCs) to carry the green fluorescent protein (GFP) gene to cutaneous injury sites in a murine model. Read More

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http://dx.doi.org/10.1186/s12860-017-0140-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5446710PMC
May 2017
26 Reads

Adenylyl cyclase mRNA localizes to the posterior of polarized DICTYOSTELIUM cells during chemotaxis.

BMC Cell Biol 2017 05 25;18(1):23. Epub 2017 May 25.

Laboratory of Cellular and Molecular Biology, Center for Cancer Research, 37 Convent Drive, Bldg.37/Rm2066, NCI, NIH, Bethesda, MD, 20892-4256, USA.

Background: In Dictyostelium discoideum, vesicular transport of the adenylyl cyclase A (ACA) to the posterior of polarized cells is essential to relay exogenous 3',5'-cyclic adenosine monophosphate (cAMP) signals during chemotaxis and for the collective migration of cells in head-to-tail arrangements called streams.

Results: Using fluorescence in situ hybridization (FISH), we discovered that the ACA mRNA is asymmetrically distributed at the posterior of polarized cells. Using both standard estimators and Monte Carlo simulation methods, we found that the ACA mRNA enrichment depends on the position of the cell within a stream, with the posterior localization of ACA mRNA being strongest for cells at the end of a stream. Read More

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http://dx.doi.org/10.1186/s12860-017-0139-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5445419PMC
May 2017
15 Reads

ULK1 phosphorylates Sec23A and mediates autophagy-induced inhibition of ER-to-Golgi traffic.

BMC Cell Biol 2017 05 10;18(1):22. Epub 2017 May 10.

School of Biomedical Sciences, Shatin, N.T., Hong Kong, Special Administrative Region of China.

Background: Autophagy is an inducible autodigestive process that allows cells to recycle proteins and other materials for survival during stress and nutrient deprived conditions. The kinase ULK1 is required to activate this process. ULK1 phosphorylates a number of target proteins and regulates many cellular processes including the early secretory pathway. Read More

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http://dx.doi.org/10.1186/s12860-017-0138-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5424413PMC
May 2017
31 Reads

Loss of pericyte smoothened activity in mice with genetic deficiency of leptin.

BMC Cell Biol 2017 04 20;18(1):20. Epub 2017 Apr 20.

Department of Medicine, Division of Gastroenterology, Duke University, 905 S. LaSalle Street, Snyderman Building, Suite 1073, Durham, NC, 27710, USA.

Background: Obesity is associated with multiple diseases, but it is unclear how obesity promotes progressive tissue damage. Recovery from injury requires repair, an energy-expensive process that is coupled to energy availability at the cellular level. The satiety factor, leptin, is a key component of the sensor that matches cellular energy utilization to available energy supplies. Read More

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http://dx.doi.org/10.1186/s12860-017-0135-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5399438PMC
April 2017
10 Reads

Intermittent parathyroid hormone (PTH) promotes cementogenesis and alleviates the catabolic effects of mechanical strain in cementoblasts.

BMC Cell Biol 2017 04 20;18(1):19. Epub 2017 Apr 20.

Department of Orthodontics, West China Hospital of Stomatology, Sichuan University, No. 14, 3rd Section, Renmin South Road, Chengdu, 610041, China.

Background: External root resorption, commonly starting from cementum, is a severe side effect of orthodontic treatment. In this pathological process and repairing course followed, cementoblasts play a significant role. Previous studies implicated that parathyroid hormone (PTH) could act on committed osteoblast precursors to promote differentiation, and inhibit apoptosis. Read More

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http://dx.doi.org/10.1186/s12860-017-0133-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5397739PMC
April 2017
18 Reads

Dental pulp pluripotent-like stem cells (DPPSC), a new stem cell population with chromosomal stability and osteogenic capacity for biomaterials evaluation.

BMC Cell Biol 2017 04 21;18(1):21. Epub 2017 Apr 21.

Regenerative Medicine Research Institute, Universitat Internacional de Catalunya, Barcelona, Spain.

Background: Biomaterials are widely used to regenerate or substitute bone tissue. In order to evaluate their potential use for clinical applications, these need to be tested and evaluated in vitro with cell culture models. Frequently, immortalized osteoblastic cell lines are used in these studies. Read More

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http://dx.doi.org/10.1186/s12860-017-0137-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5399345PMC
April 2017
22 Reads

Stress induced nuclear granules form in response to accumulation of misfolded proteins in Caenorhabditis elegans.

BMC Cell Biol 2017 04 19;18(1):18. Epub 2017 Apr 19.

Department of Biology, Middle Tennessee State University, 1301 E. Main Street, Murfreesboro, TN, 37132, USA.

Background: Environmental stress can affect the viability or fecundity of an organism. Environmental stressors may affect the genome or the proteome and can cause cellular distress by contributing to protein damage or misfolding. This study examines the cellular response to environmental stress in the germline of the nematode, C. Read More

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http://dx.doi.org/10.1186/s12860-017-0136-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5395811PMC
April 2017
6 Reads

Impact of the MDM2 splice-variants MDM2-A, MDM2-B and MDM2-C on cytotoxic stress response in breast cancer cells.

BMC Cell Biol 2017 04 17;18(1):17. Epub 2017 Apr 17.

Section of Oncology, Department of Clinical Science, University of Bergen, 5020, Bergen, Norway.

Background: The murine double minute 2 (MDM2) is an oncogene and a negative regulator of the tumor suppressor protein p53. MDM2 is known to be amplified in numerous human cancers, and upregulation of MDM2 is considered to be an alternative mechanism of p53 inactivation. The presence of many splice variants of MDM2 has been observed in both normal tissues and malignant cells; however their impact and functional properties in response to chemotherapy treatment are not fully understood. Read More

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http://bmccellbiol.biomedcentral.com/articles/10.1186/s12860
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http://dx.doi.org/10.1186/s12860-017-0134-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5393014PMC
April 2017
8 Reads

Testis-specific ATP synthase peripheral stalk subunits required for tissue-specific mitochondrial morphogenesis in Drosophila.

BMC Cell Biol 2017 03 23;18(1):16. Epub 2017 Mar 23.

Department of Biology, Davidson College, Davidson, NC, USA.

Background: In Drosophila early post-meiotic spermatids, mitochondria undergo dramatic shaping into the Nebenkern, a spherical body with complex internal structure that contains two interwrapped giant mitochondrial derivatives. The purpose of this study was to elucidate genetic and molecular mechanisms underlying the shaping of this structure.

Results: The knotted onions (knon) gene encodes an unconventionally large testis-specific paralog of ATP synthase subunit d and is required for internal structure of the Nebenkern as well as its subsequent disassembly and elongation. Read More

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http://dx.doi.org/10.1186/s12860-017-0132-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5364652PMC
March 2017
21 Reads