Search our Database of Scientific Publications and Authors

I’m looking for a

    1307 results match your criteria BMC Biotechnology [Journal]

    1 OF 27

    Agrobacterium-mediated and electroporation-mediated transformation of Chlamydomonas reinhardtii: a comparative study.
    BMC Biotechnol 2018 Feb 17;18(1):11. Epub 2018 Feb 17.
    ENEA, Italian National Agency for New Technologies, Energy and Sustainable Economic Development, Casaccia Research Center, 00123, Rome, Italy.
    Background: Chlamydomonas reinhardtii is an unicellular green alga used for functional genomics studies and heterologous protein expression. A major hindrance in these studies is the low level and instability of expression of nuclear transgenes, due to their rearrangement and/or silencing over time.

    Results: We constructed dedicated vectors for Agrobacterium-mediated transformation carrying, within the T-DNA borders, the Paromomycin (Paro) selectable marker and an expression cassette containing the Luciferase (Luc) reporter gene. Read More

    Effects of substrate binding site residue substitutions of xynA from Bacillus amyloliquefaciens on substrate specificity.
    BMC Biotechnol 2018 Feb 13;18(1). Epub 2018 Feb 13.
    P. G. Department of Biosciences, UGC-Centre of advanced studies, Satellite campus, Sardar Patel University, Sardar Patel Maidan, Bakrol-Vadtal Road, PO Box 39, Vallabh Vidyanagar, Gujarat, 388 120, India.
    Background: The aromatic residues of xylanase enzyme, W187, Y124, W144, Y128 and W63 of substrate binding pocket from Bacillus amyloliquefaciens were investigated for their role in substrate binding by homology modelling and sequence analysis. These residues are highly conserved and play an important role in substrate binding through steric hindrance. The substitution of these residues with alanine allows the enzyme to accommodate nonspecific substrates. Read More

    Genetically encoded calcium indicator with NTnC-like design and enhanced fluorescence contrast and kinetics.
    BMC Biotechnol 2018 Feb 13;18(1):10. Epub 2018 Feb 13.
    Moscow Institute of Physics and Technology, Moscow, 123182, Russia.
    Background: The recently developed genetically encoded calcium indicator (GECI), called NTnC, has a novel design with reduced size due to utilization of the troponin C (TnC) as a Ca-binding moiety inserted into the mNeonGreen fluorescent protein. NTnC binds two times less Caions while maintaining a higher fluorescence brightness at the basal level of Cain neurons as compared with the calmodulin-based GECIs, such as GCaMPs. In spite of NTnC's high brightness, pH-stability, and high sensitivity to single action potentials, it has a limited fluorescence contrast (F/F) and slow Cadissociation kinetics. Read More

    A new approach for detecting adventitious viruses shows Sf-rhabdovirus-negative Sf-RVN cells are suitable for safe biologicals production.
    BMC Biotechnol 2018 Feb 7;18(1). Epub 2018 Feb 7.
    GlycoBac LLC, 1938 Harney Street, Laramie, WY, 82072, USA.
    Background: Adventitious viral contamination in cell substrates used for biologicals production is a major safety concern. A powerful new approach that can be used to identify adventitious viruses is a combination of bioinformatics tools with massively parallel sequencing technology. Typically, this involves mapping or BLASTN searching individual reads against viral nucleotide databases. Read More

    DNA methylome profiling at single-base resolution through bisulfite sequencing of 5mC-immunoprecipitated DNA.
    BMC Biotechnol 2018 Feb 6;18(1). Epub 2018 Feb 6.
    Department of Stomatology, Chinese PLA General Hospital, Beijing, 100853, People's Republic of China.
    Background: Detection of DNA methylome at single-base resolution is a significant challenge but promises to shed considerable light on human disease etiology. Current technologies could not detect DNA methylation genome-wide at single-base resolution with small amount of sequencing data and could not avoid detecting the methylation of repetitive elements which are considered as "junk DNA".

    Methods: In this study, we have developed a novel DNA methylome profiling technology named MB-seq with its ability to identify genome-wide 5mC and quantify DNA methylation levels by introduced an assistant adapter AluI-linker This linker can be ligated to sonicated DNA and then be digested after the bisulfite treatment and amplification, which has no effect of MeDIP enrichment. Read More

    Two approaches for estimating the lower limit of quantitation (LLOQ) of microRNA levels assayed as exploratory biomarkers by RT-qPCR.
    BMC Biotechnol 2018 Feb 2;18(1). Epub 2018 Feb 2.
    Center for Drug Evaluation and Research, Food and Drug Administration, Silver Spring, MD, 20993, USA.
    Background: Circulating microRNAs are undergoing exploratory use as safety biomarkers in drug development. Reverse transcription quantitative polymerase chain reaction (RT-qPCR) is one common approach used to quantitate levels of microRNAs in samples that includes the use of a standard curve of calibrators fit to a regression model. Guidelines are needed for setting assay quantitation thresholds that are appropriate for this method and to biomarker pre-validation. Read More

    Genetic engineering of Escherichia coli to improve L-phenylalanine production.
    BMC Biotechnol 2018 Jan 30;18(1). Epub 2018 Jan 30.
    Tianjin Institutes of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, People's Republic of China.
    Background: L-phenylalanine (L-Phe) is an essential amino acid for mammals and applications expand into human health and nutritional products. In this study, a system level engineering was conducted to enhance L-Phe biosynthesis in Escherichia coli.

    Results: We inactivated the PTS system and recruited glucose uptake via combinatorial modulation of galP and glk to increase PEP supply in the Xllp01 strain. Read More

    Optimized DNA electroporation for primary human T cell engineering.
    BMC Biotechnol 2018 Jan 30;18(1). Epub 2018 Jan 30.
    Laboratory of Vaccine and Antibody Engineering, Beijing Institute of Biotechnology, No. 20, Dongdajie street, Fengtai District, Beijing, 100071, China.
    Background: Effective gene-delivery systems for primary human T cell engineering are useful tools for both basic research and clinical immunotherapy applications. Pseudovirus-based systems and electro-transfection are the most popular strategies for genetic material transduction. Compared with viral-particle-mediated approaches, electro-transfection is theoretically safer, because it does not promote transgene integration into the host genome. Read More

    RNase I-treated quantitative PCR for dsRNA quantitation of RNAi trait in genetically modified crops.
    BMC Biotechnol 2018 Jan 17;18(1). Epub 2018 Jan 17.
    Dow AgroSciences LLC, 9330 Zionsville Rd, Indianapolis, IN, 46268, USA.
    Background: RNA interference (RNAi) technology has been widely used to knockdown target genes via post-transcriptional silencing. In plants, RNAi is used as an effective tool with diverse applications being developed such as resistance against insects, fungi, viruses, and metabolism manipulation. To develop genetically modified (GM) RNAi traits for insect control, a transgene is created and composed of an inversely-repeated sequence of the target gene with a spacer region inserted between the repeats. Read More

    Donor age affects proteome composition of tenocyte-derived engineered tendon.
    BMC Biotechnol 2018 Jan 16;18(1). Epub 2018 Jan 16.
    Institute of Ageing and Chronic Disease, University of Liverpool, William Duncan Building, 6 West Derby Street, Liverpool, L7 8TX, UK.
    Background: The concept of tissue engineering is to deliver to the injury site biological scaffolds carrying functional cells that will enhance healing response. The preferred cell source is autologous in order to reduce immune response in the treated individual. However, in elderly patients age-related changes in synthetic activity of the implanted cells and subsequent alterations in tissue protein content may affect therapeutic outcomes. Read More

    The function of tcf3 in medaka embryos: efficient knockdown with pePNAs.
    BMC Biotechnol 2018 Jan 9;18(1). Epub 2018 Jan 9.
    Department for Applied Life Sciences, University of Applied Sciences, FH Campus Wien, Helmut-Qualtinger-Gasse 2, A-1030, Vienna, Austria.
    Background: The application of antisense molecules, such as morpholino oligonucleotides, is an efficient method of gene inactivation in vivo. We recently introduced phosphonic ester modified peptide nucleic acids (PNA) for in vivo loss-of-function experiments in medaka embryos. Here we tested novel modifications of the PNA backbone to knockdown the medaka tcf3 gene. Read More

    Laser-fabricated cell patterning stencil for single cell analysis.
    BMC Biotechnol 2017 Dec 19;17(1):89. Epub 2017 Dec 19.
    Center for Biosignatures Discovery Automation, The Biodesign Institute, Arizona State University, 1001 S. McAllister Ave., P.O. Box 877101, Tempe, AZ, 85287-7101, USA.
    Precise spatial positioning and isolation of mammalian cells is a critical component of many single cell experimental methods and biological engineering applications. Although a variety of cell patterning methods have been demonstrated, many of these methods subject cells to high stress environments, discriminate against certain phenotypes, or are a challenge to implement. Here, we demonstrate a rapid, simple, indiscriminate, and minimally perturbing cell patterning method using a laser fabricated polymer stencil. Read More

    Computer face-matching technology using two-dimensional photographs accurately matches the facial gestalt of unrelated individuals with the same syndromic form of intellectual disability.
    BMC Biotechnol 2017 Dec 19;17(1):90. Epub 2017 Dec 19.
    Imagus Technology, Brisbane, QLD, Australia.
    Background: Massively parallel genetic sequencing allows rapid testing of known intellectual disability (ID) genes. However, the discovery of novel syndromic ID genes requires molecular confirmation in at least a second or a cluster of individuals with an overlapping phenotype or similar facial gestalt. Using computer face-matching technology we report an automated approach to matching the faces of non-identical individuals with the same genetic syndrome within a database of 3681 images [1600 images of one of 10 genetic syndrome subgroups together with 2081 control images]. Read More

    Modulation of the regioselectivity of Thermomyces lanuginosus lipase via biocatalyst engineering for the Ethanolysis of oil in fully anhydrous medium.
    BMC Biotechnol 2017 Dec 16;17(1):88. Epub 2017 Dec 16.
    Department of Biocatalysis, Institute of Catalysis and Petrochemistry (ICP) CSIC. Campus UAM, Cantoblanco, 28049, Madrid, Spain.
    Background: Enzymatic ethanolysis of oils (for example, high oleic sunflower oil containing 90% of oleic acid) may yield two different reaction products depending on the regioselectivity of the immobilized lipase biocatalyst. Some lipase biocatalysts exhibit a 1,3-regioselectivity and they produced 2 mols of fatty acid ethyl ester plus 1 mol of sn2-monoacylglycerol (2-MAG) per mol of triglyceride without the release of glycerol. Other lipase biocatalysts are completely non-regioselective releasing 3 mols of fatty acid ethyl ester and 1 mol of glycerol per mol of triglyceride. Read More

    Heterologous expression, purification and function of the extracellular domain of human RANK.
    BMC Biotechnol 2017 Dec 4;17(1):87. Epub 2017 Dec 4.
    Key Laboratory of Stem Cell Biology, Shanghai Institutes for Biological Sciences, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai, 200031, China.
    Background: Receptor activator of NF-κB ligand (RANKL)/RANK signaling essentially functions within the skeletal system, particularly participating in osteoclastogenesis and bone resorption. In addition, this signaling pathway has also been shown to influence tumor progression as well as the development and function of the immune system. Therefore, blocking the interaction between RANKL and RANK is a new therapeutic approach to prevent bone-related diseases and cancer. Read More

    A tandem CBM25 domain of α-amylase from Microbacterium aurum as potential tool for targeting proteins to starch granules during starch biosynthesis.
    BMC Biotechnol 2017 Dec 4;17(1):86. Epub 2017 Dec 4.
    Wageningen University and Research, Plant Breeding, P.O. Box 386, 6700, AJ, Wageningen, The Netherlands.
    Background: Starch-binding domains from carbohydrate binding module family 20 have been used as a tool for starch engineering. Previous studies showed that expression of starch binding domain fusion proteins in planta resulted in modified starch granule structures and physicochemical properties. However, although 13 carbohydrate binding module families have been reported to contain starch-binding domains, only starch-binding domains from carbohydrate binding module family 20 have been well studied and introduced into plants successfully. Read More

    Optimal reduction of chemical oxygen demand and NH-N from landfill leachate using a strongly resistant novel Bacillus salmalaya strain.
    BMC Biotechnol 2017 Nov 28;17(1):85. Epub 2017 Nov 28.
    Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia.
    Background: When the unavoidable waste generation is considered as damaging to our environment, it becomes crucial to develop a sustainable technology to remediate the pollutant source towards an environmental protection and safety. The development of a bioengineering technology for highly efficient pollutant removal is this regard. Given the high ammonia nitrogen content and chemical oxygen demand of landfill leachate, Bacillus salmalaya strain 139SI, a novel resident strain microbe that can survive in high ammonia nitrogen concentrations, was investigated for the bioremoval of ammonia nitrogen from landfill leachate. Read More

    Characterization of a novel bioflocculant from a marine bacterium and its application in dye wastewater treatment.
    BMC Biotechnol 2017 Nov 17;17(1):84. Epub 2017 Nov 17.
    Department of Chemical and Biochemical Engineering, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, People's Republic of China.
    Background: The identification of microorganisms with excellent flocculant-producing capability and optimization of the fermentation process are necessary for the wide-scale application of bioflocculants. Thus, we evaluated the flocculant-producing ability of a novel strain identified by the screening of marine bacteria, and we report for the first time the properties of the bioflocculant produced by Alteromonas sp. in the treatment of dye wastewater. Read More

    A new single-step protocol for rapid baculovirus-driven protein production in insect cells.
    BMC Biotechnol 2017 Nov 16;17(1):83. Epub 2017 Nov 16.
    Max-Planck Institute of Biochemistry, Am Klopferspitz 18, 82152, Martinsried, Germany.
    Background: In the last three decades, the Baculovirus expression vector system (BEV) has evolved to one of the most widely used eukaryotic systems for heterologous protein expression including approved vaccines and therapies. Despite the significant improvements introduced during the past years, the BEV system still has major drawbacks, primarily the time required to generate recombinant virus and virus instability for certain target proteins. In this study we show that the conventional method to generate recombinant Baculovirus using a Tn7 transposition based system can be shortened to a single-step transfection-only procedure without further amplification. Read More

    Comparison of [HSO], [Cl]and [MeCO]as anions in pretreatment of aspen and spruce with imidazolium-based ionic liquids.
    BMC Biotechnol 2017 Nov 15;17(1):82. Epub 2017 Nov 15.
    Department of Chemistry, Umeå University, SE-901 87, Umeå, Sweden.
    Background: Ionic liquids (ILs) draw attention as green solvents for pretreatment of lignocellulose before enzymatic saccharification. Imidazolium-based ILs with different anionic constituents ([HSO], [Cl], [MeCO]) were compared with regard to pretreatment of wood from aspen and spruce. The objective was to elucidate how the choice of anionic constituent affected the suitability of using the IL for pretreatment of hardwood, such as aspen, and softwood, such as spruce. Read More

    AFEAP cloning: a precise and efficient method for large DNA sequence assembly.
    BMC Biotechnol 2017 Nov 14;17(1):81. Epub 2017 Nov 14.
    McGovern Medical School, the University of Texas Health Science Center at Houston, Houston, 77030, USA.
    Background: Recent development of DNA assembly technologies has spurred myriad advances in synthetic biology, but new tools are always required for complicated scenarios. Here, we have developed an alternative DNA assembly method named AFEAP cloning (Assembly of Fragment Ends After PCR), which allows scarless, modular, and reliable construction of biological pathways and circuits from basic genetic parts.

    Methods: The AFEAP method requires two-round of PCRs followed by ligation of the sticky ends of DNA fragments. Read More

    Adaptation of the GoldenBraid modular cloning system and creation of a toolkit for the expression of heterologous proteins in yeast mitochondria.
    BMC Biotechnol 2017 Nov 13;17(1):80. Epub 2017 Nov 13.
    Centro de Biotecnología y Genómica de Plantas, Universidad Politécnica de Madrid (UPM) - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Campus Montegancedo UPM, 28223, Pozuelo de Alarcón, Madrid, Spain.
    Background: There is a need for the development of synthetic biology methods and tools to facilitate rapid and efficient engineering of yeast that accommodates the needs of specific biotechnology projects. In particular, the manipulation of the mitochondrial proteome has interesting potential applications due to its compartmentalized nature. One of these advantages resides in the fact that metalation occurs after protein import into mitochondria, which contains pools of iron, zinc, copper and manganese ions that can be utilized in recombinant metalloprotein metalation reactions. Read More

    Optimization of physical conditions for the production of thermostable T1 lipase in Pichia guilliermondii strain SO using response surface methodology.
    BMC Biotechnol 2017 Nov 10;17(1):78. Epub 2017 Nov 10.
    Enzyme and Microbial Technology Research Center, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
    Background: Pichia guilliermondii was found capable of expressing the recombinant thermostable lipase without methanol under the control of methanol dependent alcohol oxidase 1 promoter (AOXp 1). In this study, statistical approaches were employed for the screening and optimisation of physical conditions for T1 lipase production in P. guilliermondii. Read More

    Production and purification of chimeric HBc virus-like particles carrying influenza virus LAH domain as vaccine candidates.
    BMC Biotechnol 2017 Nov 10;17(1):79. Epub 2017 Nov 10.
    iQur Limited, 2 Royal College Street, London, NW1 0NH, UK.
    Background: The lack of a universal influenza vaccine is a global health problem. Interest is now focused on structurally conserved protein domains capable of eliciting protection against a broad range of influenza virus strains. The long alpha helix (LAH) is an attractive vaccine component since it is one of the most conserved influenza hemagglutinin (HA) stalk regions. Read More

    Successful production of the potato antimicrobial peptide Snakin-1 in baculovirus-infected insect cells and development of specific antibodies.
    BMC Biotechnol 2017 Nov 9;17(1):75. Epub 2017 Nov 9.
    Instituto de Biotecnología, Centro de Investigación en Ciencias Veterinarias y Agronómicas, Centro Nacional de Investigaciones Agropecuarias, Instituto Nacional de Tecnología Agropecuaria, Repetto y De Los Reseros s/n, CP 1686, Hurlingham, Buenos Aires, Argentina.
    Background: Snakin-1 (StSN1) is a broad-spectrum antimicrobial cysteine-rich peptide isolated from Solanum tuberosum. Its biotechnological potential has been already recognized since it exhibits in vivo antifungal and antibacterial activity. Most attempts to produce StSN1, or homologous peptides, in a soluble native state using bacterial, yeast or synthetic expression systems have presented production bottlenecks such as insolubility, misfolding or low yields. Read More

    Generation of murine macrophage-derived cell lines expressing porcine CD163 that support porcine reproductive and respiratory syndrome virus infection.
    BMC Biotechnol 2017 Nov 9;17(1):77. Epub 2017 Nov 9.
    Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, China.
    Background: Porcine reproductive and respiratory syndrome virus (PRRSV) exhibits a highly restricted tropism for cells of the monocyte-macrophage lineage, utilizing porcine CD163 (pCD163) as an indispensable cellular receptor for infection. Transfection the gene of pCD163 into several non-permissive cell lines followed by protein expression confers susceptibility to PRRSV. A lack of specialized porcine antibody tools for use with existing porcine-derived primary cells and cell lines has hampered studies of both PRRSV pathogenesis and virus triggering of immune response cascades. Read More

    Biological activities and chemical compositions of slime tracks and crude exopolysaccharides isolated from plasmodia of Physarum polycephalum and Physarella oblonga.
    BMC Biotechnol 2017 Nov 9;17(1):76. Epub 2017 Nov 9.
    School of Biotechnology, International University, VNU-HCM, Block 6, LinhTrung Ward, Thu Duc District, Ho Chi Minh City, 70000, Vietnam.
    Background: The myxomycetes derive their common name (slime molds) from the multinucleate trophic stage (plasmodium) in the life cycle, which typically produces a noticeable amount of slimy materials, some of which is normally left behind as a "slime track" as the plasmodium migrates over the surface of a particular substrate. The study reported herein apparently represents the first attempt to investigate the chemical composition and biological activities of slime tracks and the exopolysaccharides (EPS) which cover the surface of the plasmodia of Physarum polycephalum and Physarella oblonga.

    Results: Chemical analyses indicated that the slime tracks and samples of the EPS consist largely of carbohydrates, proteins and various sulphate groups. Read More

    Light induced expression of β-glucosidase in Escherichia coli with autolysis of cell.
    BMC Biotechnol 2017 Nov 7;17(1):74. Epub 2017 Nov 7.
    School of Life Sciences, Anhui University, Hefei, Anhui, 230601, China.
    Background: β-Glucosidase has attracted substantial attention in the scientific community because of its pivotal role in cellulose degradation, glycoside transformation and many other industrial processes. However, the tedious and costly expression and purification procedures have severely thwarted the industrial applications of β-glucosidase. Thus development of new strategies to express β-glucosidases with cost-effective and simple procedure to meet the increasing demands on enzymes for biocatalysis is of paramount importance. Read More

    De novo characterization of venom apparatus transcriptome of Pardosa pseudoannulata and analysis of its gene expression in response to Bt protein.
    BMC Biotechnol 2017 Nov 7;17(1):73. Epub 2017 Nov 7.
    College of Bioscience & Biotechnology, Hunan Agriculture University, Changsha, 410128, China.
    Background: Pardosa pseudoannulata is a prevailing spider species, and has been regarded as an important bio-control agent of insect pests in farmland of China. However, the available genomic and transcriptomic databases of P. pseudoannulata and their venom are limited, which severely hampers functional genomic analysis of P. Read More

    Simultaneous detection of α-Lactoalbumin, β-Lactoglobulin and Lactoferrin in milk by Visualized Microarray.
    BMC Biotechnol 2017 Sep 12;17(1):72. Epub 2017 Sep 12.
    State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210093, China.
    Background: α-Lactalbumin (a-LA), β-lactoglobulin (β-LG) and lactoferrin (LF) are of high nutritional value which have made ingredients of choice in the formulation of modern foods and beverages. There remains an urgent need to develop novel biosensing methods for quantification featuring reduced cost, improved sensitivity, selectivity and more rapid response, especially for simultaneous detection of multiple whey proteins.

    Results: A novel visualized microarray method was developed for the determination of a-LA, β-LG and LF in milk samples without the need for complex or time-consuming pre-treatment steps. Read More

    Effect of supercritical carbon dioxide on the enzymatic production of biodiesel from waste animal fat using immobilized Candida antarctica lipase B variant.
    BMC Biotechnol 2017 Sep 9;17(1):70. Epub 2017 Sep 9.
    SKKU Advanced Institute of Nanotechnology, Sungkyunkwan University, 2066 Seobu-ro, Jangan-gu, Suwon-si, Gyeonggi-do, 16419, South Korea.
    Background: Waste animal fat is a promising feedstock to replace vegetable oil that widely used in commercial biodiesel process, however the high content of free fatty acid in waste fat makes it unfeasible to be processed with commercial base-catalytic process. Enzymatic process is preferable to convert waste fat into biodiesel since enzyme can catalyze both esterification of free fatty acid and transesterification of triglyceride. However, enzymatic reaction still has some drawbacks such as lower reaction rates than base-catalyzed transesterification and the limitation of reactant concentration due to the enzyme inhibition of methanol. Read More

    Conversion of an inactive xylose isomerase into a functional enzyme by co-expression of GroEL-GroES chaperonins in Saccharomyces cerevisiae.
    BMC Biotechnol 2017 Sep 9;17(1):71. Epub 2017 Sep 9.
    Laboratory of Genomics and Expression, Department of Genetics and Evolution, Institute of Biology, UNICAMP, Campinas, São Paulo, 13083-970, Brazil.
    Background: Second-generation ethanol production is a clean bioenergy source with potential to mitigate fossil fuel emissions. The engineering of Saccharomyces cerevisiae for xylose utilization is an essential step towards the production of this biofuel. Though xylose isomerase (XI) is the key enzyme for xylose conversion, almost half of the XI genes are not functional when expressed in S. Read More

    A split ubiquitin system to reveal topology and released peptides of membrane proteins.
    BMC Biotechnol 2017 Sep 2;17(1):69. Epub 2017 Sep 2.
    Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai, 200438, China.
    Background: Membrane proteins define biological functions of membranes in cells. Extracellular peptides of transmembrane proteins receive signals from pathogens or environments, and are the major targets of drug developments. Despite of their essential roles, membrane proteins remain elusive in topological studies due to technique difficulties in their expressions and purifications. Read More

    Expression of Vitreoscilla hemoglobin enhances production of arachidonic acid and lipids in Mortierella alpina.
    BMC Biotechnol 2017 Aug 30;17(1):68. Epub 2017 Aug 30.
    Shandong Provincial Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No.189 Songling Road, Laoshan District, Qingdao, Shandong Province, 266101, China.
    Background: Arachidonic acid (ARA, C20:4, n-6), which belongs to the omega-6 series of polyunsaturated fatty acids and has a variety of biological activities, is commercially produced in Mortierella alpina. Dissolved oxygen or oxygen utilization efficiency is a critical factor for Mortierella alpina growth and arachidonic acid production in large-scale fermentation. Overexpression of the Vitreoscilla hemoglobin gene is thought to significantly increase the oxygen utilization efficiency of the cells. Read More

    A bioinformatics approach for identifying transgene insertion sites using whole genome sequencing data.
    BMC Biotechnol 2017 Aug 15;17(1):67. Epub 2017 Aug 15.
    Department of Agriculture and Life Industry, Kangwon National University, 1 Kangwondaehak-gil, Chuncheon, Gangwon, 24341, Republic of Korea.
    Background: Genetically modified crops (GM crops) have been developed to improve the agricultural traits of modern crop cultivars. Safety assessments of GM crops are of paramount importance in research at developmental stages and before releasing transgenic plants into the marketplace. Sequencing technology is developing rapidly, with higher output and labor efficiencies, and will eventually replace existing methods for the molecular characterization of genetically modified organisms. Read More

    An in vitro synthetic biosystem based on acetate for production of phloroglucinol.
    BMC Biotechnol 2017 Aug 8;17(1):66. Epub 2017 Aug 8.
    CAS Key Laboratory of Bio-Based Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101, China.
    Background: Phloroglucinol is an important chemical, and the biosynthesis processes which can convert glucose to phloroglucinol have been established. However, due to approximate 80% of the glucose being transformed into undesirable by-products and biomass, this biosynthesis process only shows a low yield with the highest value of about 0.20 g/g. Read More

    Immobilisation of Delta-like 1 ligand for the scalable and controlled manufacture of hematopoietic progenitor cells in a stirred bioreactor.
    BMC Biotechnol 2017 Aug 4;17(1):65. Epub 2017 Aug 4.
    Healthcare Engineering Research Group, Centre for Biological Engineering, Wolfson School of Mechanical and Manufacturing Engineering, Loughborough University, Loughborough, Leicestershire, UK.
    Background: Umbilical cord blood provides a source of hematopoietic stem cells for transplantation with immunological and availability advantages over conventional bone marrow sources. Limited cell numbers and slower engraftment from umbilical cord blood units has led to the clinical development of immobilised Notch ligand Delta-Like 1 to promote ex vivo expansion of a rapidly engrafting cell population. However, current immobilisation methods are not simple to scale in a controlled manner. Read More

    Comprehensive in silico allergenicity assessment of novel protein engineered chimeric Cry proteins for safe deployment in crops.
    BMC Biotechnol 2017 Aug 2;17(1):64. Epub 2017 Aug 2.
    ICAR-National Research Centre on Plant Biotechnology, LBS Centre, Pusa Campus, New Delhi, 110012, India.
    Background: Development of chimeric Cry toxins by protein engineering of known and validated proteins is imperative for enhancing the efficacy and broadening the insecticidal spectrum of these genes. Expression of novel Cry proteins in food crops has however created apprehensions with respect to the safety aspects. To clarify this, premarket evaluation consisting of an array of analyses to evaluate the unintended effects is a prerequisite to provide safety assurance to the consumers. Read More

    Effect of acetic acid on ethanol production by Zymomonas mobilis mutant strains through continuous adaptation.
    BMC Biotechnol 2017 Aug 1;17(1):63. Epub 2017 Aug 1.
    Department of Microbiology, Immunology and Biopharmaceuticals, National Chiayi University, Chiayi, Taiwan.
    Background: Acetic acid is a predominant by-product of lignocellulosic biofuel process, which inhibits microbial biocatalysts. Development of bacterial strains that are tolerant to acetic acid is challenging due to poor understanding of the underlying molecular mechanisms.

    Results: In this study, we generated and characterized two acetic acid-tolerant strains of Zymomonas mobilis using N-methyl-N'-nitro-N-nitrosoguanidine (NTG)-acetate adaptive breeding. Read More

    An att site-based recombination reporter system for genome engineering and synthetic DNA assembly.
    BMC Biotechnol 2017 Jul 14;17(1):62. Epub 2017 Jul 14.
    Unité Plasticité du Génome Bactérien, Département Génomes et Génétique, Institut Pasteur, 75015, Paris, France.
    Background: Direct manipulation of the genome is a widespread technique for genetic studies and synthetic biology applications. The tyrosine and serine site-specific recombination systems of bacteriophages HK022 and ΦC31 are widely used for stable directional exchange and relocation of DNA sequences, making them valuable tools in these contexts. We have developed site-specific recombination tools that allow the direct selection of recombination events by embedding the attB site from each system within the β-lactamase resistance coding sequence (bla). Read More

    Overview of methodologies for T-cell receptor repertoire analysis.
    BMC Biotechnol 2017 Jul 10;17(1):61. Epub 2017 Jul 10.
    Institute of Clinical Molecular Biology, Kiel University, Rosalind-Franklin-Str. 12, 24105, Kiel, Germany.
    Background: The T-cell receptor (TCR), located on the surface of T cells, is responsible for the recognition of the antigen-major histocompatibility complex, leading to the initiation of an inflammatory response. Analysing the TCR repertoire may help to gain a better understanding of the immune system features and of the aetiology and progression of diseases, in particular those with unknown antigenic triggers. The extreme diversity of the TCR repertoire represents a major analytical challenge; this has led to the development of specialized methods which aim to characterize the TCR repertoire in-depth. Read More

    Unusual acylation of chloramphenicol in Lysobacter enzymogenes, a biocontrol agent with intrinsic resistance to multiple antibiotics.
    BMC Biotechnol 2017 07 4;17(1):59. Epub 2017 Jul 4.
    Department of Chemistry, University of Nebraska-Lincoln, Lincoln, NE, 68588-0304, USA.
    Background: The environmental gliding bacteria Lysobacter are emerging as a new group of biocontrol agents due to their prolific production of lytic enzymes and potent antibiotic natural products. These bacteria are intrinsically resistant to many antibiotics, but the mechanisms behind the antibiotic resistance have not been investigated.

    Results: Previously, we have used chloramphenicol acetyltransferase gene (cat) as a selection marker in genetic manipulation of natural product biosynthetic genes in Lysobacter, because chloramphenicol is one of the two common antibiotics that Lysobacter are susceptible to. Read More

    Heterogeneous oxygen availability affects the titer and topology but not the fidelity of plasmid DNA produced by Escherichia coli.
    BMC Biotechnol 2017 07 4;17(1):60. Epub 2017 Jul 4.
    Departamento de Procesos y Tecnología, Universidad Autónoma Metropolitana-Cuajimalpa, Av. Vasco de Quiroga 4871, Col. Santa Fe, 05348, Mexico City, Mexico.
    Background: Dissolved oxygen tension (DOT) is hardly constant and homogenously distributed in a bioreactor, which can have a negative impact in the metabolism and product synthesis. However, the effects of DOT on plasmid DNA (pDNA) production and quality have not been thoroughly investigated. In the present study, the effects of aerobic (DOT ≥30% air sat. Read More

    Potent long-acting rhFGF21 analog for treatment of diabetic nephropathy in db/db and DIO mice.
    BMC Biotechnol 2017 07 4;17(1):58. Epub 2017 Jul 4.
    School of Pharmacy, Wenzhou Medical University, Wenzhou, 325035, China.
    Background: Fibroblast growth factor 21 (FGF21) is an endocrine-acting hormone that has the potential to treat diabetic nephropathy. However, development of FGF21 into a therapeutic has been hindered due to its low intrinsic bio-stability. In our previous study, we have developed a recombinant human FGF21 (rhFGF21) variant by site-directed mutagenesis and solid-phase PEGylation, which retained its biological function. Read More

    Site-specific chemical conjugation of human Fas ligand extracellular domain using trans-cyclooctene - methyltetrazine reactions.
    BMC Biotechnol 2017 07 3;17(1):56. Epub 2017 Jul 3.
    Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Central 6, 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8566, Japan.
    Background: Fas ligand plays a key role in the human immune system as a major cell death inducing protein. The extracellular domain of human Fas ligand (hFasLECD) triggers apoptosis of malignant cells, and therefore is expected to have substantial potentials in medical biotechnology. However, the current application of this protein to clinical medicine is hampered by a shortage of the benefits relative to the drawbacks including the side-effects in systemic administration. Read More

    Oligomerization triggered by foldon: a simple method to enhance the catalytic efficiency of lichenase and xylanase.
    BMC Biotechnol 2017 07 3;17(1):57. Epub 2017 Jul 3.
    Fujian Provincial Key Laboratory of Biochemical Technology, Huaqiao University, Xiamen, Fujian, 361021, China.
    Background: Effective and simple methods that lead to higher enzymatic efficiencies are highly sough. Here we proposed a foldon-triggered trimerization of the target enzymes with significantly improved catalytic performances by fusing a foldon domain at the C-terminus of the enzymes via elastin-like polypeptides (ELPs). The foldon domain comprises 27 residues and can forms trimers with high stability. Read More

    Continuous long-term cytotoxicity monitoring in 3D spheroids of beetle luciferase-expressing hepatocytes by nondestructive bioluminescence measurement.
    BMC Biotechnol 2017 06 20;17(1):54. Epub 2017 Jun 20.
    Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Takamatsu, Kagawa, 761-0395, Japan.
    Background: Three-dimensional (3D) spheroids are frequently used in toxicological study because their morphology and function closely resemble those of tissue. As these properties are maintained over a long term, repeated treatment of the spheroids with a test object is possible. Generally, in the repeated treatment test to assess cytotoxicity in the spheroids, ATP assay, colorimetric measurement using pigments or high-content imaging analysis is performed. Read More

    Enhancement of thermoalkaliphilic xylanase production by Pichia pastoris through novel fed-batch strategy in high cell-density fermentation.
    BMC Biotechnol 2017 06 21;17(1):55. Epub 2017 Jun 21.
    Laboratory of Feed Biotechnology, State Key Laboratory of Animal Nutrition, College of Animal Science & Technology, China Agricultural University, No.2 Yuanmingyuan West Road, Haidian District, Beijing, 100193, China.
    Background: Xylanase degrades xylan into monomers of various sizes by catalyzing the endohydrolysis of the 1,4-β-D-xylosidic linkage randomly, possessing potential in wide industrial applications. Most of xylanases are susceptible to be inactive when suffering high temperature and high alkaline process. Therefore, it is necessary to develop a high amount of effective thermoalkaliphilic xylanases. Read More

    A hyper-thermostable α-amylase from Pyrococcus furiosus accumulates in Nicotiana tabacum as functional aggregates.
    BMC Biotechnol 2017 06 19;17(1):53. Epub 2017 Jun 19.
    Agriculture and Agri-Food Canada, London Research and Development Centre, London, Ontario, Canada.
    Background: Alpha amylase hydrolyzes α-bonds of polysaccharides such as starch and produces malto-oligosaccharides. Its starch saccharification applications make it an essential enzyme in the textile, food and brewing industries. Commercially available α-amylase is mostly produced from Bacillus or Aspergillus. Read More

    Expression and functional analyses of a Kinesin gene GhKIS13A1 from cotton (Gossypium hirsutum) fiber.
    BMC Biotechnol 2017 06 12;17(1):50. Epub 2017 Jun 12.
    The Key Laboratory of Oasis Eco-agriculture, Agriculture College, Shihezi University, Bei 4 Road, Shihezi, 832003, Xinjiang, China.
    Background: Cotton fiber, a natural fiber widely used in the textile industry, is differentiated from single cell of ovule epidermis. A large number of genes are believed to be involved in fiber formation, but so far only a few fiber genes have been isolated and functionally characterized in this developmental process. The Kinesin13 subfamily was found to play key roles during cell division and cell elongation, and was considered to be involved in the regulation of cotton fiber development. Read More

    1 OF 27