1,508 results match your criteria BMC Biotechnology [Journal]


Recombinant PAL/PilE/FlaA DNA vaccine provides protective immunity against Legionella pneumophila in BALB/c mice.

BMC Biotechnol 2020 May 18;20(1):28. Epub 2020 May 18.

Department of Pulmonary and Critical Care Medicine, Shengjing Hospital of China Medical University, 36 Sanhao Street, Shenyang, 110004, People's Republic of China.

Background: Legionella pneumophila (L.pneumophila), a Gram-negative small microorganism, causes hospital-acquired pneumonia especially in immunocompromised patients. Vaccination may be an effective method for preventing L. Read More

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http://dx.doi.org/10.1186/s12896-020-00620-3DOI Listing

Screening of five marine-derived fungal strains for their potential to produce oxidases with laccase activities suitable for biotechnological applications.

BMC Biotechnol 2020 May 12;20(1):27. Epub 2020 May 12.

Ecole Nationale d'Ingénieurs de Sfax, Laboratoire de Biochimie et de Génie enzymatique des lipases, Université de Sfax, Sfax, Tunisie.

Background: Environmental pollution is one of the major problems that the world is facing today. Several approaches have been taken, from physical and chemical methods to biotechnological strategies (e.g. Read More

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http://dx.doi.org/10.1186/s12896-020-00617-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7218534PMC

GoldenBac: a simple, highly efficient, and widely applicable system for construction of multi-gene expression vectors for use with the baculovirus expression vector system.

BMC Biotechnol 2020 May 12;20(1):26. Epub 2020 May 12.

Protein Technologies Facility, Vienna BioCenter Core Facilities, Dr. Bohr-gasse 3, 1030, Vienna, Austria.

Background: Recombinant protein production and purification of large protein complexes in eukaryotes requires efficient methods to generate multi-gene expression constructs, where each individual gene is under the control of its own promoter and terminator. Current methods are based either on serial rounds of combination of several vectors containing loxP sites via the Cre-lox technology, or on multiple rounds of gene combination via PCR or other methods. These methods are multi-step, have lower efficiencies than single gene cloning, and may require laborious processes to verify that all genes of interest are present in the final product. Read More

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http://dx.doi.org/10.1186/s12896-020-00616-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7216392PMC

Generation of transgene-free PDS mutants in potato by Agrobacterium-mediated transformation.

BMC Biotechnol 2020 May 12;20(1):25. Epub 2020 May 12.

NARIC Agricultural Biotechnology Institute, H-2100 Szent-Györgyi A. u. 4., Gödöllő, Hungary.

Background: Gene editing using the CRISPR/Cas9 system has become a routinely applied method in several plant species. The most convenient gene delivery system is Agrobacterium-mediated gene transfer with antibiotic selection and stable genomic integration of transgenes, including Cas9. For elimination of transgenes in the segregating progeny, selfing is applied in many plant species. Read More

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http://dx.doi.org/10.1186/s12896-020-00621-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7216596PMC

Translational regulation of periplasmic folding assistants and proteases as a valuable strategy to improve production of translocated recombinant proteins in Escherichia coli.

BMC Biotechnol 2020 May 11;20(1):24. Epub 2020 May 11.

Department of Biotechnology and Food Science, Norwegian University of Science and Technology, Sem Sælandsvei 6-8, N-7491, Trondheim, Norway.

Background: Advantages of translocation of recombinant proteins to the periplasm in Escherichia coli include simplified downstream processing, and improved folding and in vivo activity of the target protein. There are, however, problems encountered in the periplasmic production that can be associated with the incorrect formation of disulfide bonds, incomplete cleavage of the signal peptide, and proteolytic degradation. A common strategy used to overcome these difficulties involves manipulating the cellular levels of proteases and periplasmic folding assistants like chaperones, signal peptide peptidases or thiol-disulfide oxidoreductases. Read More

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http://dx.doi.org/10.1186/s12896-020-00615-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7216727PMC

Retraction Note: DNA methylome profiling at single-base resolution through bisulfite sequencing of 5mC-immunoprecipitated DNA.

BMC Biotechnol 2020 May 11;20(1):23. Epub 2020 May 11.

Department of Stomatology, Chinese PLA General Hospital, Beijing, 100853, People's Republic of China.

An amendment to this paper has been published and can be accessed via the original article. Read More

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http://dx.doi.org/10.1186/s12896-020-00619-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7216471PMC
May 2020
2.034 Impact Factor

Utilization of egg-laying hens (Gallus Gallus domesticus) for production of ingredients for human consumption and animal feed.

BMC Biotechnol 2020 May 6;20(1):22. Epub 2020 May 6.

SINTEF Sealab, Brattørkaia 17 C, 7010, Trondheim, Norway.

Background: In Norway, 3 million discarded egg-laying hens are destructed annually, which equals 1500 tons pure hen meat. Due to the slaughter methods used, this raw material is handled as a high-risk waste, while in reality it constitutes a source of valuable components like proteins and lipids.

Methods: This study assess different processing methods (thermal treatment, enzymatic hydrolysis and silaging) for utilization of discarded egg-laying hens for the production of ingredients for human consumption and animal feed. Read More

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http://dx.doi.org/10.1186/s12896-020-00618-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7204061PMC

Process optimization and effect of thermal, alkaline, HO oxidation and combination pretreatment of sewage sludge on solubilization and anaerobic digestion.

BMC Biotechnol 2020 May 6;20(1):21. Epub 2020 May 6.

Faculty of Engineeringss, Department of Civil Engineering, Monash University, Melbourne, Australia.

Background: This study investigated the feasibility of enhancing anaerobic digestion of sewage sludge with triple, dual, and individual pretreatment of waste activated sludge with heat, alkalinity, and hydrogen peroxide. These pretreatments disrupt sludge flocs, organisms' cell walls, extracellular polymeric substance, and intracellular organic matter, which increase biodegradability and hydrolysis rate of activate sludge. In addition, the influence of various variables on methane production was analyzed using the response surface methodology with the quadratic model. Read More

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http://dx.doi.org/10.1186/s12896-020-00614-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7201573PMC

Phytoremediation effect of Medicago sativa colonized by Piriformospora indica in the phenanthrene and cadmium co-contaminated soil.

BMC Biotechnol 2020 Apr 28;20(1):20. Epub 2020 Apr 28.

School of Chemical Engineering and Technology, Hebei University of Technology, Tianjin, 300130, China.

Background: The coexistence of polycyclic aromatic hydrocarbons (PAHs) and heavy metals has deleterious effects on environmental quality. Few reports have studied the mechanisms of plant inoculation with Piriformospora indica to remediate PAH-metal co-contaminated soil by analyzing the chemical speciation of the contaminants. This study investigated the influence of the inoculation of Medicago sativa with P. Read More

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http://dx.doi.org/10.1186/s12896-020-00613-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7187505PMC

Lentivirus expressing shRNAs inhibit the replication of contagious ecthyma virus by targeting DNA polymerase gene.

BMC Biotechnol 2020 Mar 23;20(1):18. Epub 2020 Mar 23.

Department of Microbiology, School of Biology, College of Science, University of Tehran, Tehran, Iran.

Background: Contagious ecthyma or Orf is known as a zoonotic disease remains prevalently worldwide despite the application of some control strategies against it. RNAi particularly shRNA provides us with the chance to tackle this obstacle by an encouraging new approach. The current study indicates the design and experiment of third-generation lentivirus packaging systems delivering shRNAs to inhibit Orf virus (ORFV) replication and infection. Read More

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http://dx.doi.org/10.1186/s12896-020-00611-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7092477PMC

Generation of an engineered food-grade Lactococcus lactis strain for production of an antimicrobial peptide: in vitro and in silico evaluation.

BMC Biotechnol 2020 03 30;20(1):19. Epub 2020 Mar 30.

Department of Animal Sciences, Faculty of Agriculture, Ferdowsi University of Mashhad, P.O. Box 91775-1163, Mashhad, Iran.

Background: Foodborne pathogens and their biofilms are considered as one of the most serious problems in human health and food industry. Moreover, safety of foods is a main global concern because of the increasing use of chemical food additives. Ensuring food safety enhances interest in discovery of new alternative compounds such as antimicrobial peptides (AMPs), which can be used as bio-preservatives in the food industry. Read More

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http://dx.doi.org/10.1186/s12896-020-00612-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7106598PMC

A novel family of expression vectors with multiple affinity tags for wheat germ cell-free protein expression.

BMC Biotechnol 2020 03 14;20(1):17. Epub 2020 Mar 14.

Department of Medical Chemistry, Molecular Biology and Pathobiochemistry, Semmelweis University, 37-47 Tűzoltó Street, Budapest, H-1094, Hungary.

Background: Cell-free protein expression has become a widely used alternative of in vivo, cell-based systems in functional and structural studies of proteins. The wheat germ-based method outstands from the commercially available eukaryotic in vitro translation systems by its flexibility, high translation efficiency and success rate of properly folded eukaryotic protein synthesis. The original T7 promoter containing pEU3-NII vector was improved previously by addition of a ligation-independent cloning site, His- and GST-tags, and a TEV protease cleavage site to facilitate the creation of recombinant plasmids, permit affinity purification, and enable production of purified, tag-free target proteins, respectively. Read More

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http://dx.doi.org/10.1186/s12896-020-00610-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7071761PMC

Development and biological activity of long-acting recombinant human interferon-α2b.

BMC Biotechnol 2020 03 13;20(1):16. Epub 2020 Mar 13.

NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, 100052, China.

Background: The type I human interferon (IFN) family consists of a group of cytokines with a multiplicity of biological activities, including antiviral, antitumor, and immunomodulatory effects. However, because the half-life of IFN is short, its clinical application is limited. Increasing the yield and biological activity of IFN while extending its half-life is currently the focus of IFN research. Read More

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http://dx.doi.org/10.1186/s12896-020-00605-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7071744PMC

Optimised production of an anti-fungal antibody in Solanaceae hairy roots to develop new formulations against Candida albicans.

BMC Biotechnol 2020 03 12;20(1):15. Epub 2020 Mar 12.

Department of Sustainability, Laboratory Biotechnologies, ENEA, Casaccia Research Center, Via Anguillarese 301, 00123, Rome, Italy.

Background: Infections caused by fungi are often refractory to conventional therapies and urgently require the development of novel options, such as immunotherapy. To produce therapeutic antibodies, a plant-based expression platform is an attractive biotechnological strategy compared to mammalian cell cultures. In addition to whole plants, hairy roots (HR) cultures can be used, representing an expression system easy to build up, with indefinite growth while handled under containment conditions. Read More

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http://dx.doi.org/10.1186/s12896-020-00607-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7069033PMC

An efficient gene disruption method for the woody plant pathogen Botryosphaeria dothidea.

BMC Biotechnol 2020 03 5;20(1):14. Epub 2020 Mar 5.

Laboratory of Mycology, College of Plant Protection, China Agricultural University, Beijing, 100193, China.

Background: Botryosphaeria dothidea causes apple white rot and infects many tree plants. Genome data for B. dothidea are available and many pathogenesis-related genes have been predicted. Read More

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http://dx.doi.org/10.1186/s12896-020-00608-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7059327PMC

A snapshot of microbial diversity and function in an undisturbed sugarcane bagasse pile.

BMC Biotechnol 2020 02 28;20(1):12. Epub 2020 Feb 28.

Queensland University of Technology, 2 George St, Brisbane, QLD, 4000, Australia.

Background: Sugarcane bagasse is a major source of lignocellulosic biomass, yet its economic potential is not fully realised. To add value to bagasse, processing is needed to gain access to the embodied recalcitrant biomaterials. When bagasse is stored in piles in the open for long periods it is colonised by microbes originating from the sugarcane, the soil nearby or spores in the environment. Read More

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http://dx.doi.org/10.1186/s12896-020-00609-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7049217PMC
February 2020

Cassava/peanut intercropping improves soil quality via rhizospheric microbes increased available nitrogen contents.

BMC Biotechnol 2020 02 28;20(1):13. Epub 2020 Feb 28.

Agricultural College of Guangxi University, Nanning, 530005, Guangxi, China.

Background: Intercropping, an essential cultivation pattern in modern agricultural systems, increases crop yields and soil quality. Cassava and peanut intercropping systems exhibit advantages in solar utilization and cadmium absorption, etc. However, the inner mechanisms need to be elucidated. Read More

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http://dx.doi.org/10.1186/s12896-020-00606-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7049180PMC
February 2020

Integration, abundance, and transmission of mutations and transgenes in a series of CRISPR/Cas9 soybean lines.

BMC Biotechnol 2020 02 24;20(1):10. Epub 2020 Feb 24.

Bioinformatics and Computational Biology Program, University of Minnesota, Minneapolis, MN, USA.

Background: As with many plant species, current genome editing strategies in soybean are initiated by stably transforming a gene that encodes an engineered nuclease into the genome. Expression of the transgene results in a double-stranded break and repair at the targeted locus, oftentimes resulting in mutation(s) at the intended site. As soybean is a self-pollinating species with 20 chromosome pairs, the transgene(s) in the T0 plant are generally expected to be unlinked to the targeted mutation(s), and the transgene(s)/mutation(s) should independently assort into the T1 generation, resulting in Mendellian combinations of transgene presence/absence and allelic states within the segregating family. Read More

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http://dx.doi.org/10.1186/s12896-020-00604-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7038615PMC
February 2020

Soft - sensing modeling based on ABC - MLSSVM inversion for marine low - temperature alkaline protease MP fermentation process.

BMC Biotechnol 2020 02 18;20(1). Epub 2020 Feb 18.

School of Electrical and Information Engineering, JiangSu University, Zhenjiang, 212013, Jiangsu, China.

Background: Aiming at the characteristics of nonlinear, multi-parameter, strong coupling and difficulty in direct on-line measurement of key biological parameters of marine low-temperature protease fermentation process, a soft-sensing modeling method based on artificial bee colony (ABC) and multiple least squares support vector machine (MLSSVM) inversion for marine protease fermentation process is proposed.

Methods: Firstly, based on the material balance and the characteristics of the fermentation process, the dynamic "grey box" model of the fed-batch fermentation process of marine protease is established. The inverse model is constructed by analyzing the inverse system existence and introducing the characteristic information of the fermentation process. Read More

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http://dx.doi.org/10.1186/s12896-020-0603-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7029510PMC
February 2020

Trauma induced tissue survival in vitro with a muscle-biomaterial based osteogenic organoid system: a proof of concept study.

BMC Biotechnol 2020 01 31;20(1). Epub 2020 Jan 31.

Department of Orthopedics, Physical Medicine and Rehabilitation, University Hospital of Munich (LMU), Munich, Germany.

Background: The translation from animal research into the clinical environment remains problematic, as animal systems do not adequately replicate the human in vivo environment. Bioreactors have emerged as a good alternative that can reproduce part of the human in vivo processes at an in vitro level. However, in vitro bone formation platforms primarily utilize stem cells only, with tissue based in vitro systems remaining poorly investigated. Read More

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http://dx.doi.org/10.1186/s12896-020-0602-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6995208PMC
January 2020

Generation of recombinant baculovirus expressing atoxic C-terminal CPA toxin of Clostridium perfringens and production of specific antibodies.

BMC Biotechnol 2020 01 28;20(1). Epub 2020 Jan 28.

Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche "Togo Rosati", Via G. Salvemini 1, 06126, Perugia, Italy.

Background: Clostridium perfringens is the causative agent of several diseases and enteric infections in animals and humans. The virulence of C. perfringens is largely attributable to the production of numerous toxins; of these, the alpha toxin (CPA) plays a crucial role in histotoxic infections (gas gangrene). Read More

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http://dx.doi.org/10.1186/s12896-019-0597-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6986089PMC
January 2020

Production, purification and characterization of recombinant human R-spondin1 (RSPO1) protein stably expressed in human HEK293 cells.

BMC Biotechnol 2020 01 20;20(1). Epub 2020 Jan 20.

Cell and Molecular Therapy Center (NUCEL), Medical School, University of São Paulo, Edifício NUCEL, Rua Pangaré, 100 (Cidade Universitária), São Paulo, SP, 05360-130, Brazil.

Background: The R-Spondin proteins comprise a family of secreted proteins, known for their important roles in cell proliferation, differentiation and death, by inducing the Wnt pathway. Several studies have demonstrated the importance of RSPOs in regulation of a number of tissue-specific processes, namely: bone formation, skeletal muscle tissue development, proliferation of pancreatic β-cells and intestinal stem cells and even cancer. RSPO1 stands out among RSPOs molecules with respect to its potential therapeutic use, especially in the Regenerative Medicine field, due to its mitogenic activity in stem cells. Read More

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http://dx.doi.org/10.1186/s12896-020-0600-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6971977PMC
January 2020
2.034 Impact Factor

Auxotrophic mutations of Trichophyton rubrum created by in vitro synthesized Cas9 ribonucleoprotein.

BMC Biotechnol 2020 01 20;20(1). Epub 2020 Jan 20.

Department of Medical Mycology, Institute of Dermatology, Chinese Academy of Medical Science and Peking Union Medical College, Nanjing, Jiangsu, 210042, People's Republic of China.

Background: Trichophyton rubrum is an obligate human parasitic fungus and responsible for approximately 80-90% of dermatomycosis in human. Molecular genetic manipulations of this pathogen are challenging and available tools and protocols are only rudimentary. We adapt molecular genetics methods of well established fungal model organism, to knock out genes in T. Read More

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http://dx.doi.org/10.1186/s12896-020-0601-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6971929PMC
January 2020

A self-aggregating peptide: implications for the development of thermostable vaccine candidates.

BMC Biotechnol 2020 01 21;20(1). Epub 2020 Jan 21.

Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, CDMX 04510, Mexico City, Mexico.

Background: The use of biomaterials has been expanded to improve the characteristics of vaccines. Recently we have identified that the peptide PH from polyhedrin self-aggregates and incorporates foreign proteins to form particles. We have proposed that this peptide can be used as an antigen carrying system for vaccines. Read More

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http://dx.doi.org/10.1186/s12896-019-0592-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6971912PMC
January 2020

Construction and characterization of a high-quality cDNA library of Cymbidium faberi suitable for yeast one- and two-hybrid assays.

BMC Biotechnol 2020 01 16;20(1). Epub 2020 Jan 16.

Center of Applied Biotechnology, Wuhan University of Bioengineering, Wuhan, 430415, People's Republic of China.

Background: Cymbidium faberi is one of the oldest cultivars of oriental orchids, with an elegant flower fragrance. In order to investigate the molecular mechanism and the functions of related proteins in the methyl jasmonate (MeJA) signaling pathway, one of the main components of flower fragrance in C. faberi, yeast one- and two-hybrid three-frame cDNA libraries were constructed. Read More

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http://dx.doi.org/10.1186/s12896-020-0599-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6966867PMC
January 2020

Intein-mediated recombinant expression of monomeric B22Asp desB30 insulin.

BMC Biotechnol 2020 01 9;20(1). Epub 2020 Jan 9.

Key Laboratory of Science and Technology of Eco-Textiles, Ministry of Education, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, 2999 North Ren Min Rd, Shanghai, 201620, China.

Background: Insulin controls hyperglycemia caused by diabetes, and virtually all treatments require exogenous insulin. However, the product's extensive post-translational modifications have hindered the manufacture of recombinant insulin.

Result: Here we report a novel production method for a monomeric B22Asp desB30 insulin analog (B22D desB30 insulin). Read More

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http://dx.doi.org/10.1186/s12896-020-0598-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6953245PMC
January 2020
2.034 Impact Factor

Screening of cellulolytic bacteria from rotten wood of Qinling (China) for biomass degradation and cloning of cellulases from Bacillus methylotrophicus.

BMC Biotechnol 2020 01 7;20(1). Epub 2020 Jan 7.

Lab of Bioresources, College of Food Science and Engineering, Northwest A&F University, Yangling, 712100, Shaanxi Province, China.

Background: Cellulosic biomass degradation still needs to be paid more attentions as bioenergy is the most likely to replace fossil energy in the future, and more evaluable cellulolytic bacteria isolation will lay a foundation for this filed. Qinling Mountains have unique biodiversity, acting as promising source of cellulose-degrading bacteria exhibiting noteworthy properties. Therefore, the aim of this work was to find potential cellulolytic bacteria and verify the possibility of the cloning of cellulases from the selected powerful bacteria. Read More

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http://dx.doi.org/10.1186/s12896-019-0593-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6947901PMC
January 2020

Development and applications of a monoclonal antibody against caprine interferon-gamma.

BMC Biotechnol 2019 12 23;19(1):102. Epub 2019 Dec 23.

College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, Shaanxi Province, China.

Background: Interferon-gamma (IFN-γ) is an important mediator of type I immune response and has antiviral, immunoregulatory and anti-tumor properties, plays a wide range of roles in inflammation and autoimmune diseases. The aim of this study was to obtain monoclonal antibody (mAb) against caprine IFN-γ by immunizing of BALB/c mice with the purified rIFN-γ.

Results: Recombinant caprine IFN-γ was expressed in Escherichia coli strain BL21 (DE3) and monoclonal antibodies against caprine IFN-γ were produced by immunizing of BALB/c mice with rIFN-γ. Read More

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http://dx.doi.org/10.1186/s12896-019-0596-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6929374PMC
December 2019
5 Reads

The next generation of biopanning: next gen sequencing improves analysis of bacterial display libraries.

BMC Biotechnol 2019 12 21;19(1):100. Epub 2019 Dec 21.

Biotechnology Branch, CCDC US Army Research Laboratory, 2800 Powder Mill Rd, Adelphi, 20783, MD, USA.

Background: Bacterial surface display libraries are a popular tool for novel ligand discovery due to their ease of manipulation and rapid growth rates. These libraries typically express a scaffold protein embedded within the outer membrane with a short, surface-exposed peptide that is either terminal or is incorporated into an outer loop, and can therefore interact with and bind to substrates of interest.

Results: In this study, we employed a novel bacterial peptide display library which incorporates short 15-mer peptides on the surface of E. Read More

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http://dx.doi.org/10.1186/s12896-019-0577-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6925417PMC
December 2019

Optimized production of a biologically active Clostridium perfringens glycosyl hydrolase phage endolysin PlyCP41 in plants using virus-based systemic expression.

BMC Biotechnol 2019 12 21;19(1):101. Epub 2019 Dec 21.

USDA ARS NEA BARC Animal Biosciences and Biotechnology Laboratory, Beltsville, MD, 20705, USA.

Background: Clostridium perfringens, a gram-positive, anaerobic, rod-shaped bacterium, is the third leading cause of human foodborne bacterial disease and a cause of necrotic enteritis in poultry. It is controlled using antibiotics, widespread use of which may lead to development of drug-resistant bacteria. Bacteriophage-encoded endolysins that degrade peptidoglycans in the bacterial cell wall are potential replacements for antibiotics. Read More

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http://dx.doi.org/10.1186/s12896-019-0594-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6925876PMC
December 2019

Fecal DNA isolation and degradation in clam Cyclina sinensis: noninvasive DNA isolation for conservation and genetic assessment.

BMC Biotechnol 2019 12 19;19(1):99. Epub 2019 Dec 19.

Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang, 222005, Jiangsu, China.

Background: To avoid destructive sampling for conservation and genetic assessment, we isolated the DNA of clam Cyclina sinensis from their feces. DNA electrophoresis and PCR amplification were used to determine the quality of fecal DNA. And we analyzed the effects of different conditions on the degradation of feces and fecal DNA. Read More

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http://dx.doi.org/10.1186/s12896-019-0595-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6923993PMC
December 2019

The effect of diet and radiation on the bacterial symbiome of the melon fly, Zeugodacus cucurbitae (Coquillett).

BMC Biotechnol 2019 12 18;19(Suppl 2):88. Epub 2019 Dec 18.

Department of Environmental Engineering, University of Patras, 2 Seferi St., 30131, Agrinio, Greece.

Background: Symbiotic bacteria contribute to a multitude of important biological functions such as nutrition and reproduction and affect multiple physiological factors like fitness and longevity in their insect hosts. The melon fly, Zeugodacus cucurbitae (Coquillett), is an important agricultural pest that affects a variety of cultivated plants belonging mostly to the Cucurbitaceae family. It is considered invasive and widespread in many parts of the world. Read More

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http://dx.doi.org/10.1186/s12896-019-0578-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6918631PMC
December 2019

Potential of a fly gut microbiota incorporated gel-based larval diet for rearing Bactrocera dorsalis (Hendel).

BMC Biotechnol 2019 12 18;19(Suppl 2):94. Epub 2019 Dec 18.

Jashore University of Science and Technology, Jashore, Bangladesh.

Background: The Oriental fruit fly, Bactrocera dorsalis (Hendel) (Diptera: Tephritidae), is an important polyphagous pest of horticultural produce. The sterile insect technique (SIT) is a proven control method against many insect pests, including fruit flies, under area-wide pest management programs. High quality mass-rearing process and the cost-effective production of sterile target species are important for SIT. Read More

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http://dx.doi.org/10.1186/s12896-019-0580-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6918544PMC
December 2019

Horizontal transfer and finalization of a reliable detection method for the olive fruit fly endosymbiont, Candidatus Erwinia dacicola.

BMC Biotechnol 2019 12 18;19(Suppl 2):93. Epub 2019 Dec 18.

Department of Agriculture, Food, Environment and Forestry (DAGRI), University of Florence, via Maragliano 77, 50144, Florence, Italy.

Background: The olive fly, Bactrocera oleae, is the most important insect pest in olive production, causing economic damage to olive crops worldwide. In addition to extensive research on B. oleae control methods, scientists have devoted much effort in the last century to understanding olive fly endosymbiosis with a bacterium eventually identified as Candidatus Erwinia dacicola. Read More

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http://dx.doi.org/10.1186/s12896-019-0583-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6918556PMC
December 2019

The host fruit amplifies mutualistic interaction between Ceratitis capitata larvae and associated bacteria.

BMC Biotechnol 2019 12 18;19(Suppl 2):92. Epub 2019 Dec 18.

Departments of Plant Pathology & Microbiology, Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, POB 12, 76100, Rehovot, Israel.

Background: The Mediterranean fruit fly Ceratitis capitata is a major pest in horticulture. The development of fly larvae is mediated by bacterial decay in the fruit tissue. Despite the importance of bacteria on larval development, very little is known about the interaction between bacteria and larvae in their true ecological context. Read More

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http://dx.doi.org/10.1186/s12896-019-0581-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6918632PMC
December 2019

The oesophageal diverticulum of Dirioxa pornia studied through micro-CT scan, dissection and SEM studies.

BMC Biotechnol 2019 12 18;19(Suppl 2):89. Epub 2019 Dec 18.

School of Agriculture, Food and Wine, The University of Adelaide, Adelaide, SA, Australia.

Background: Dirioxa pornia (Diptera, Tephritidae) (Island fly) is an Australian native species related to a number of pestiferous fruit flies but, unlike many of the pest species, has not been studied extensively due to its non-pest status. However, due to D. pornia's apparent reliance on the bacteria for survival it is an ideal species to undertake studies into interaction between Tephritid species and bacteria associated with the intestinal tract. Read More

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http://dx.doi.org/10.1186/s12896-019-0585-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6918637PMC
December 2019

A walk on the wild side: gut bacteria fed to mass-reared larvae of Queensland fruit fly [Bactrocera tryoni (Froggatt)] influence development.

BMC Biotechnol 2019 12 18;19(Suppl 2):95. Epub 2019 Dec 18.

Biosecurity and Food Safety, New South Wales Department of Primary Industries, Elizabeth Macarthur Agricultural Institute, Menangle, 2568, Australia.

Background: The Queensland fruit fly, Bactrocera tryoni (Froggatt) (Diptera, Tephritidae) is the most significant insect pest of Australian horticulture. Bactrocera tryoni is controlled using a range of tools including the Sterile Insect Technique (SIT). Mass-rearing and irradiation of pupae in SIT can reduce the fitness and quality of the released sterile insects. Read More

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http://dx.doi.org/10.1186/s12896-019-0579-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6918714PMC
December 2019

Olive fruit fly rearing procedures affect the vertical transmission of the bacterial symbiont Candidatus Erwinia dacicola.

BMC Biotechnol 2019 12 18;19(Suppl 2):91. Epub 2019 Dec 18.

Department of Agriculture, Food, Environment and Forestry (DAGRI), University of Florence, via Maragliano 77, 50144, Florence, Italy.

Background: The symbiosis between the olive fruit fly, Bactrocera oleae, and Candidatus Erwinia dacicola has been demonstrated as essential for the fly's larval development and adult physiology. The mass rearing of the olive fruit fly has been hindered by several issues, including problems which could be related to the lack of the symbiont, presumably due to preservatives and antibiotics currently used during rearing under laboratory conditions. To better understand the mechanisms underlying symbiont removal or loss during the rearing of lab colonies of the olive fruit fly, we performed experiments that focused on bacterial transfer from wild female flies to their eggs. Read More

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http://dx.doi.org/10.1186/s12896-019-0582-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6918546PMC
December 2019

Medfly-Wolbachia symbiosis: genotype x genotype interactions determine host's life history traits under mass rearing conditions.

BMC Biotechnol 2019 12 18;19(Suppl 2):96. Epub 2019 Dec 18.

Laboratory of Entomology and Agricultural Zoology, Department of Agriculture Crop Production and Rural Environment, University of Thessaly, Phytokou St., 38446 N, Ionia Magnisia, Greece.

Background: Wolbachia pipientis is a widespread, obligatory intracellular and maternally inherited bacterium, that induces a wide range of reproductive alterations to its hosts. Cytoplasmic Incompatibility (CI) is causing embryonic lethality, the most common of them. Despite that Wolbachia-borne sterility has been proposed as an environmental friendly pest control method (Incompatible Insect Technique, IIT) since 1970s, the fact that Wolbachia modifies important fitness components of its hosts sets severe barriers to IIT implementation. Read More

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http://dx.doi.org/10.1186/s12896-019-0586-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6918550PMC
December 2019

Biochemical and nutritional characterization of the medfly gut symbiont Enterobacter sp. AA26 for its use as probiotics in sterile insect technique applications.

BMC Biotechnol 2019 12 18;19(Suppl 2):90. Epub 2019 Dec 18.

Laboratory of Wastewater Management and Treatment Technologies, Department of Environmental Engineering, Democritus University of Thrace, Vas. Sofias 12, 67100, Xanthi, Greece.

Background: Enterobacter sp. AA26 was recently isolated from the midgut of Ceratitis capitata (Wiedemann) and it was shown to have positive effects in rearing efficiency when used as larval probiotics. In this study, biomass production was carried out in bench-scale bioreactors to elucidate the biokinetic properties of Enterobacter sp. Read More

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http://dx.doi.org/10.1186/s12896-019-0584-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6918548PMC
December 2019

Simultaneous hydrolysis with lipase and fermentation of rapeseed cake for iturin A production by Bacillus amyloliquefaciens CX-20.

BMC Biotechnol 2019 12 16;19(1):98. Epub 2019 Dec 16.

Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan, 430062, People's Republic of China.

Background: Rapeseed cake (RSC), as the intermediate by-product of oil extraction from the seeds of Brassica napus, can be converted into rapeseed meal (RSM) by solvent extraction to remove oil. However, compared with RSM, RSC has been rarely used as a raw material for microbial fermentation, although both RSC and RSM are mainly composed of proteins, carbohydrates and minerals. In this study, we investigated the feasibility of using untreated low-cost RSC as nitrogen source to produce the valuable cyclic lipopeptide antibiotic iturin A using Bacillus amyloliquefaciens CX-20 in submerged fermentation. Read More

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http://dx.doi.org/10.1186/s12896-019-0591-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6915999PMC
December 2019

Morphological analysis of Apolipoprotein E binding to Aβ Amyloid using a combination of Surface Plasmon Resonance, Immunogold Labeling and Scanning Electron Microscopy.

BMC Biotechnol 2019 12 11;19(1):97. Epub 2019 Dec 11.

Department of Medical Biochemistry and Biophysics, Umeå University, SE-901 87, Umeå, Sweden.

Background: Immunogold labeling in combination with transmission electron microscopy analysis is a technique frequently used to correlate high-resolution morphology studies with detailed information regarding localization of specific antigens. Although powerful, the methodology has limitations and it is frequently difficult to acquire a stringent system where unspecific low-affinity interactions are removed prior to analysis.

Results: We here describe a combinatorial strategy where surface plasmon resonance and immunogold labeling are used followed by a direct analysis of the sensor-chip surface by scanning electron microscopy. Read More

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http://dx.doi.org/10.1186/s12896-019-0589-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6907347PMC
December 2019

A new method for quantitative detection of Lactobacillus casei based on casx gene and its application.

BMC Biotechnol 2019 12 10;19(1):87. Epub 2019 Dec 10.

Institute of Food Science and Technology, Chinese Academy of Agricultural Science, Beijing, 100193, China.

Background: The traditional method of bacterial identification based on 16S rRNA is a widely used and very effective detection method, but this method still has some deficiencies, especially in the identification of closely related strains. A high homology with little differences is mostly observed in the 16S sequence of closely related bacteria, which results in difficulty to distinguish them by 16S rRNA-based detection method. In order to develop a rapid and accurate method of bacterial identification, we studied the possibility of identifying bacteria with other characteristic fragments without the use of 16S rRNA as detection targets. Read More

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http://dx.doi.org/10.1186/s12896-019-0587-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6902566PMC
December 2019

Purification of the recombinant green fluorescent protein from tobacco plants using alcohol/salt aqueous two-phase system and hydrophobic interaction chromatography.

BMC Biotechnol 2019 12 9;19(1):86. Epub 2019 Dec 9.

Key Laboratory of Ministry of Education for Protection and Utilization of Special Biological Resources in the Western China, Yinchuan, 750021, People's Republic of China.

Background: The green fluorescent protein (GFP) has been regarded as a valuable tool and widely applied as a biomarker in medical applications and diagnostics. A cost-efficient upstream expression system and an inexpensive downstream purification process will meet the demands of the GFP protein with high-purity.

Results: The recombinant GFP was transiently expressed in an active form in agoinoculated Nicotiana benthamiana leaves by using Tobacco mosaic virus (TMV) RNA-based overexpression vector (TRBO). Read More

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http://dx.doi.org/10.1186/s12896-019-0590-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6902424PMC
December 2019

Improvement and use of CRISPR/Cas9 to engineer a sperm-marking strain for the invasive fruit pest Drosophila suzukii.

BMC Biotechnol 2019 12 5;19(1):85. Epub 2019 Dec 5.

Department of Developmental Biology, Johann-Friedrich-Blumenbach-Institute of Zoology and Anthropology, Göttingen Center for Molecular Biosciences, Georg-August-University Göttingen, 37077, Göttingen, Germany.

Background: The invasive fruit pest Drosophila suzukii was reported for the first time in Europe and the USA in 2008 and has spread since then. The adoption of type II clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) as a tool for genome manipulation provides new ways to develop novel biotechnologically-based pest control approaches. Stage or tissue-specifically expressed genes are of particular importance in the field of insect biotechnology. Read More

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http://dx.doi.org/10.1186/s12896-019-0588-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6896403PMC
December 2019

Tuning the performance of CAR T cell immunotherapies.

BMC Biotechnol 2019 11 29;19(1):84. Epub 2019 Nov 29.

Department of Microbiology, Immunology and Biochemistry, University of Tennessee Health Science Center, 858 Madison Avenue, Memphis, TN, 38163, USA.

Background: Simultaneous advances in gene editing, T cell engineering and biotechnology currently provide an opportunity for rapid progress in medicine. The approval of chimeric antigen receptor (CAR) T cell therapies by the US Food and Drug Administration (FDA) and the European Commission have generated substantial momentum for these first-in-class therapies to be used in patients with B cell malignancies.

Main Body: Considerable efforts focus on improved outcomes and reduced side effects of the newly approved therapies. Read More

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http://dx.doi.org/10.1186/s12896-019-0576-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6884819PMC
November 2019

LC-MSMS based screening of emerging pollutant degradation by different peroxidases.

BMC Biotechnol 2019 11 28;19(1):83. Epub 2019 Nov 28.

Department of Chemistry, College of Arts and Sciences, Khalifa University, P O Box 127788, Abu Dhabi, UAE.

Background: The presence of a wide range of bioactive organic pollutants in wastewater and municipal water sources is raising concerns about their potential effects on humans. Not surprisingly, various approaches are being explored that can efficiently degrade these persistent organic pollutants. Use of peroxidases has recently been recognized as a novel remediation approach that may have potential advantages over conventional degradation techniques. Read More

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http://dx.doi.org/10.1186/s12896-019-0574-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6883607PMC
November 2019
2.034 Impact Factor

Engineering integrative vectors based on phage site-specific recombination mechanism for Lactococcus lactis.

BMC Biotechnol 2019 11 27;19(1):82. Epub 2019 Nov 27.

Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia.

Background: Site-specific integration system allows foreign DNA to be integrated into the specific site of the host genome, enabling stable expression of heterologous protein. In this study, integrative vectors for secretion and surface display of proteins were constructed based on a lactococcal phage TP901-1 integrating system.

Results: The constructed integration system comprises of a lactococcal promoter (P or P), phage attachment site (attP) from bacteriophage TP901-1, a signal peptide (USP45 or SPK1) for translocation of the target protein, and a PrtP anchor domain in the case of the integrative vectors for surface display. Read More

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http://dx.doi.org/10.1186/s12896-019-0575-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6882331PMC
November 2019

Production and characterization of virus-like particles of grapevine fanleaf virus presenting L2 epitope of human papillomavirus minor capsid protein.

BMC Biotechnol 2019 11 21;19(1):81. Epub 2019 Nov 21.

Laboratory for the Analysis of Medicines (LAM), Department of Pharmaceutical Sciences, CIRM, University of Liège, Quartier Hôpital, B36, Tower 4, Avenue Hippocrate, 15, 4000, Liège, Belgium.

Background: Virus-like particle (VLP) platform represents a promising approach for the generation of efficient and immunogenic subunit vaccines. Here, the feasibility of using grapevine fanleaf virus (GFLV) VLPs as a new carrier for the presentation of human papillomavirus (HPV) L2 epitope was studied. To achieve this goal, a model of the HPV L2 epitope secondary structure was predicted and its insertion within 5 external loops in the GFLV capsid protein (CP) was evaluated. Read More

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http://dx.doi.org/10.1186/s12896-019-0566-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6868843PMC
November 2019
2.034 Impact Factor