1,392 results match your criteria BMC Biotechnology [Journal]


A new prokaryotic expression vector for the expression of antimicrobial peptide abaecin using SUMO fusion tag.

BMC Biotechnol 2019 Feb 15;19(1):13. Epub 2019 Feb 15.

MicroSynbiotiX Ltd, 11011 N Torrey Pines Rd Ste. #135, La Jolla, CA, 92037, USA.

Background: Despite the growing demand for antimicrobial peptides (AMPs) for clinical use as an alternative approach against antibiotic-resistant bacteria, the manufacture of AMPs relies on expensive, small-scale chemical methods. The small ubiquitin-related modifier (SUMO) tag is industrially practical for increasing the yield of recombinant proteins by increasing solubility and preventing degradation in expression systems.

Results: A new vector system, pKSEC1, was designed to produce AMPs, which can work in prokaryotic systems such as Escherichia coli and plant chloroplasts. Read More

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http://dx.doi.org/10.1186/s12896-019-0506-xDOI Listing
February 2019

Correction to: characterizing active transportation mechanisms for free fatty acids and antibiotics in Synechocystis sp. PCC 6803.

BMC Biotechnol 2019 Feb 13;19(1):12. Epub 2019 Feb 13.

School of Life Sciences, Arizona State University, 427 E. Tyler Mall, Tempe, AZ, 85287, USA.

Following publication of the original article [1], the author reported that the gene/protein names of slr2131 and sll0180 were swapped in the Discussion section. The details of the correction are mentioned below. Read More

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http://dx.doi.org/10.1186/s12896-019-0505-yDOI Listing
February 2019

Production of high titer of citric acid from inulin.

BMC Biotechnol 2019 Feb 11;19(1):11. Epub 2019 Feb 11.

Department of Biotechnology and Food Microbiology, Wroclaw University of Environmental and Life Sciences, 37 Chełmońskiego St, 51-630, Wroclaw, Poland.

Background: Citric acid is considered as the most economically feasible product of microbiological production, therefore studies on cheap and renewable raw materials for its production are highly desirable. In this study citric acid was synthesized by genetically engineered strains of Yarrowia lipolytica from widely available, renewable polysaccharide - inulin. Hydrolysis of inulin by the Y. Read More

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http://dx.doi.org/10.1186/s12896-019-0503-0DOI Listing
February 2019

Resveratrol alleviates lipopolysaccharide-induced inflammation in PC-12 cells and in rat model.

BMC Biotechnol 2019 Jan 28;19(1):10. Epub 2019 Jan 28.

Department of Spinal Surgery, Dalian Municipal Central Hospital, No.826, Xinan Road, Shahekou District, Dalian, 116033, Liaoning, China.

Background: Spinal cord injury (SCI) remains a huge medical problem nowadays as there is no hospital providing the versatile strategies for repairing central nervous system and restoring function. Herein, we focused on PC-12 cells as an important research tool and studied the potential role of resveratrol (RSV) in inflammation induced by LPS.

Results: RSV improved inflammatory injury and functional recovery in rat model of SCI. Read More

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http://dx.doi.org/10.1186/s12896-019-0502-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6350285PMC
January 2019

CRISPR-Cas9 mediated targeted disruption of FAD2-2 microsomal omega-6 desaturase in soybean (Glycine max.L).

BMC Biotechnol 2019 Jan 28;19(1). Epub 2019 Jan 28.

College of Agronomy, Plant Biotechnology Center, Jilin Agricultural University, Changchun, 130118, Jilin, China.

Background: Recent innovation in the field of genome engineering encompasses numerous levels of plant genome engineering which attract the substantial excitement of plant biologist worldwide. RNA-guided CRISPR Cas9 system has appeared a promising tool in site-directed mutagenesis due to its innovative utilization in different branches of biology. CRISPR-Cas9 nuclease system have supersedes all previously existed strategies and their associated pitfalls encountered with site-specific mutagenesis. Read More

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https://bmcbiotechnol.biomedcentral.com/articles/10.1186/s12
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http://dx.doi.org/10.1186/s12896-019-0501-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6350355PMC
January 2019
5 Reads

Extracellular overproduction of E7 oncoprotein of Iranian human papillomavirus type 16 by genetically engineered Lactococcus lactis.

BMC Biotechnol 2019 Jan 24;19(1). Epub 2019 Jan 24.

Department of Food Hygiene, Faculty of Veterinary Sciences, Science and Research Branch, Islamic Azad University, Tehran, IR, Iran.

Background: We aimed at constructing Lactococcus lactis strains expressing HPV-16 recombinant E7 (rE7) oncoprotein and examining its overproduction ability followed by optimizing batch and fed-batch fermentations. Thereafter, in order to assess the immunogenicity of recombinant L. lactis cells, C57BL/6 mice were immunized by oral gavage. Read More

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http://dx.doi.org/10.1186/s12896-019-0499-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6346575PMC
January 2019
2.034 Impact Factor

Homologous recombination-mediated targeted integration in monkey embryos using TALE nucleases.

BMC Biotechnol 2019 Jan 15;19(1). Epub 2019 Jan 15.

Yunnan Key Laboratory of Primate Biomedicine Research; Institute of Primate Translational Medicine, Kunming University of Science and Technology, Kunming, 650500, China.

Background: Non-human primate (NHP) models can closely mimic human physiological functions and are therefore highly valuable in biomedical research. Genome editing is now developing rapidly due to the precision and efficiency offered by engineered site-specific endonuclease-based systems, such as transcription activator-like effector nucleases (TALENs) and the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein-9 nuclease (Cas9) system. It has been demonstrated that these programmable nucleases can introduce genetic changes in embryos from many species including NHPs. Read More

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http://dx.doi.org/10.1186/s12896-018-0494-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6334428PMC
January 2019
1 Read
2.034 Impact Factor

MicroRNA-218 promotes early chondrogenesis of mesenchymal stem cells and inhibits later chondrocyte maturation.

BMC Biotechnol 2019 Jan 15;19(1). Epub 2019 Jan 15.

Department of Arthroplasty Surgery, Changzheng Hospital, Second Military Medical University, Shanghai, 200003, China.

Background: MicroRNAs (miRNAs) reportedly participate in the mesenchymal stem cell (MSC) chondrogenic differentiation regulation. We objected to examine how miR-218 regulate chondrogenic differentiation of synovium-derived MSCs (SDSCs) and the maturation of RCJ3.1C5. Read More

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http://dx.doi.org/10.1186/s12896-018-0496-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6334453PMC
January 2019
2 Reads

Characterizing active transportation mechanisms for free fatty acids and antibiotics in Synechocystis sp. PCC 6803.

BMC Biotechnol 2019 Jan 10;19(1). Epub 2019 Jan 10.

School of Life Sciences, Arizona State University, 427 E. Tyler Mall, Tempe, AZ, 85287, USA.

Background: Synechocystis sp. PCC 6803 is a photosynthetic bacterium that has been genetically modified to produce industrially relevant chemicals, yet efflux mechanisms have not been well elucidated. These photosynthetic organisms live in environments that are often nutrient limited; therefore, the genome of these organisms encodes far fewer proteins used for efflux of chemicals when compared to members of the Enterobacteriaceae family. Read More

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http://dx.doi.org/10.1186/s12896-019-0500-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6329066PMC
January 2019
1 Read

Evaluation of strategies for improving the transgene expression in an oleaginous microalga Scenedesmus acutus.

BMC Biotechnol 2019 Jan 10;19(1). Epub 2019 Jan 10.

Special Research Unit in Microalgal Molecular Genetics and Functional Genomics (MMGFG), Department of Genetics, Faculty of Science, Kasetsart University, 50 Ngam Wong Wan road, Chatuchak, Bangkok, 10900, Thailand.

Background: Genetic transformation of microalgae has been hampered by inefficient transgene expression, limiting the progress of microalgal biotechnology. Many vector tools and strategies have been developed in recent years to improve transgene expression in the model microalga Chlamydomonas, but these were hardly applied to other microalgae. In this work, naturally-isolated oleaginous microalgae were accessed for genetic transformation, and various expression systems were evaluated in a selected microalga to circumvent inefficient transgene expression. Read More

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http://dx.doi.org/10.1186/s12896-018-0497-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6327543PMC
January 2019
1 Read

Fast gene disruption in Trichoderma reesei using in vitro assembled Cas9/gRNA complex.

BMC Biotechnol 2019 Jan 9;19(1). Epub 2019 Jan 9.

Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, No. 12 South Zhongguancun Street, Beijing, 100081, China.

Background: CRISPR/Cas9 has wide application potentials in a variety of biological species including Trichoderma reesei, a filamentous fungus workhorse for cellulase production. However, expression of Cas9 heterologously in the host cell could be time-consuming and sometimes even troublesome.

Results: We tested two gene disruption methods in T. Read More

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http://dx.doi.org/10.1186/s12896-018-0498-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6325762PMC
January 2019
1 Read

An auto-inducible phosphate-controlled expression system of Bacillus licheniformis.

BMC Biotechnol 2019 Jan 9;19(1). Epub 2019 Jan 9.

Pharmaceutical Biotechnology, Institute of Pharmacy, University Greifswald, Greifswald, Germany.

Background: A promoter that drives high-level, long-term expression of the target gene under substrate limited growth conditions in the absence of an artificial inducer would facilitate the efficient production of heterologous proteins at low cost. A novel phosphate-regulated expression system was constructed using the promoter of the phytase encoding gene phyL from Bacillus licheniformis for the overexpression of proteins in this industrially relevant host.

Results: It is shown that the phyL promoter enables a strong overexpression of the heterologous genes amyE and xynA in B. Read More

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http://dx.doi.org/10.1186/s12896-018-0490-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6327384PMC
January 2019
1 Read

Establishment of TP53-knockout canine cells using optimized CRIPSR/Cas9 vector system for canine cancer research.

BMC Biotechnol 2019 Jan 3;19(1). Epub 2019 Jan 3.

Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul, 02841, Republic of Korea.

Background: Genetic engineering technology such as clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system provides a powerful tool for developing disease models and determining gene functions. Recent interests in canine cancer models have highlighted the necessity of developing genetic engineering tools for dogs. In this study, we attempted to generate optimized CRISPR/Cas9 system to target canine tumor protein 53 (TP53), one of the most crucial tumor suppressor genes, to establish TP53 knockout canine cells for canine cancer research. Read More

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https://bmcbiotechnol.biomedcentral.com/articles/10.1186/s12
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http://dx.doi.org/10.1186/s12896-018-0491-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6318917PMC
January 2019
5 Reads

Alterations in the proteomic composition of Serratia marcescens in response to manganese (II).

BMC Biotechnol 2018 Dec 29;18(1):83. Epub 2018 Dec 29.

Laboratório de Bioquímica e Biologia Molecular, Departamento de Ciências Biológicas (DECBI) & Instituto de Ciências Exatas e Biológica (NUPEB), Campus Morro do Cruzeiro, Universidade Federal de Ouro Preto, Ouro Preto, MG, Brazil.

Background: Proteomics is an important tool for the investigation of dynamic physiological responses of microbes under heavy metal stress. To gain insight into how bacteria respond to manganese (II) and identify the proteins involved in Mn (II) oxidation, the shotgun proteomics approach was applied to a potential Mn (II)-oxidizing Serratia marcescens strain cultivated in the absence and presence of Mn (II).

Results: The LG1 strain, which grew equally well in the two conditions, was found to express a set of proteins related to cellular processes vital for survival, as well as proteins involved in adaptation and tolerance to Mn (II). Read More

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http://dx.doi.org/10.1186/s12896-018-0493-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6311052PMC
December 2018
1 Read

A chicken bioreactor for efficient production of functional cytokines.

BMC Biotechnol 2018 Dec 29;18(1):82. Epub 2018 Dec 29.

The Roslin Institute, University of Edinburgh, Easter Bush, Midlothian, EH25 9RG, UK.

Background: The global market for protein drugs has the highest compound annual growth rate of any pharmaceutical class but their availability, especially outside of the US market, is compromised by the high cost of manufacture and validation compared to traditional chemical drugs. Improvements in transgenic technologies allow valuable proteins to be produced by genetically-modified animals; several therapeutic proteins from such animal bioreactors are already on the market after successful clinical trials and regulatory approval. Chickens have lagged behind mammals in bioreactor development, despite a number of potential advantages, due to the historic difficulty in producing transgenic birds, but the production of therapeutic proteins in egg white of transgenic chickens would substantially lower costs across the entire production cycle compared to traditional cell culture-based production systems. Read More

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http://dx.doi.org/10.1186/s12896-018-0495-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6311007PMC
December 2018
2 Reads

Enhancing the efficiency of the Pichia pastoris AOX1 promoter via the synthetic positive feedback circuit of transcription factor Mxr1.

BMC Biotechnol 2018 Dec 27;18(1):81. Epub 2018 Dec 27.

Department of Biochemical Science and Technology, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, 10617, Taiwan.

Background: The methanol-regulated AOX1 promoter (P) is the most widely used promoter in the production of recombinant proteins in the methylotrophic yeast Pichia pastoris. However, as the tight regulation and methanol dependence of P restricts its application, it is necessary to develop a flexible induction system to avoid the problems of methanol without losing the advantages of P. The availability of synthetic biology tools enables researchers to reprogram the cellular behaviour of P. Read More

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http://dx.doi.org/10.1186/s12896-018-0492-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6307218PMC
December 2018
1 Read

Recombinant Expression and Bioactivity Comparison of Four Typical Fungal Immunomodulatory Proteins from Three Main Ganoderma Species.

BMC Biotechnol 2018 Dec 14;18(1):80. Epub 2018 Dec 14.

Liaoning Province Key Laboratory of Agricultural Technology, College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang, 110866, China.

Background: More than a dozen of fungal immunomodulatory proteins (FIPs) have been identified to date, most of which are from Ganoderma species. However, little is known about the similarities and differences between different Ganoderma FIPs' bioactivities. In the current study, two FIP genes termed FIP-gap1 and FIP-gap2 from G. Read More

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http://dx.doi.org/10.1186/s12896-018-0488-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6295072PMC
December 2018
3 Reads

Simultaneous enzymatic saccharification and comminution for the valorization of lignocellulosic biomass toward natural products.

BMC Biotechnol 2018 Dec 12;18(1):79. Epub 2018 Dec 12.

Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1 Kagamiyama, Higashihiroshima, Hiroshima, 739-8530, Japan.

Background: Large-scale processing of lignocellulosics for glucose production generally relies on high temperature and acidic or alkaline conditions. However, extreme conditions produce chemical contaminants that complicate downstream processing. A method that mainly rely on mechanical and enzymatic reaction completely averts such problem and generates unmodified lignin. Read More

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http://dx.doi.org/10.1186/s12896-018-0487-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6292164PMC
December 2018
3 Reads

Association of high pressure and alkaline condition for solubilization of inclusion bodies and refolding of the NS1 protein from zika virus.

BMC Biotechnol 2018 Dec 12;18(1):78. Epub 2018 Dec 12.

Instituto de Pesquisas Energéticas e Nucleares, IPEN-CNEN/SP, Centro de Biotecnologia, Av. Prof. Lineu Prestes, 2242, São Paulo, 05508-000, Brazil.

Background: Proteins in inclusion bodies (IBs) present native-like secondary structures. However, chaotropic agents at denaturing concentrations, which are widely used for IB solubilization and subsequent refolding, unfold these secondary structures. Removal of the chaotropes frequently causes reaggregation and poor recovery of bioactive proteins. Read More

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http://dx.doi.org/10.1186/s12896-018-0486-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6291932PMC
December 2018
1 Read

High-yield production of human Dicer by transfection of human HEK293-EBNA1 cells grown in suspension.

BMC Biotechnol 2018 Dec 6;18(1):76. Epub 2018 Dec 6.

Département de Biochimie et Médecine Moléculaire, Université de Montréal, C.P. 6128, Succursale Centre-Ville, Montréal, H3C 3J7, QC, Canada.

Background: Dicer is a 219-kDa protein that plays key roles in gene regulation, particularly as the ribonuclease III enzyme responsible for cleaving precursor miRNA substrates. Its enzymatic activity is highly regulated by protein factors, and this regulation can impact on the levels of miRNAs and modulate the behavior of a cell. To better understand the underlying mechanisms of regulation, detailed enzymatic and structural characterization of Dicer are needed. Read More

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https://bmcbiotechnol.biomedcentral.com/articles/10.1186/s12
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http://dx.doi.org/10.1186/s12896-018-0485-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6282390PMC
December 2018
7 Reads

Expression of Rift Valley fever virus N-protein in Nicotiana benthamiana for use as a diagnostic antigen.

BMC Biotechnol 2018 Dec 11;18(1):77. Epub 2018 Dec 11.

Biopharming Research Unit, Department of Molecular and Cell Biology, University of Cape Town, 6503200115084, Rondebosch, Cape Town, 7700, South Africa.

Background: Rift Valley fever virus (RVFV), the causative agent of Rift Valley fever, is an enveloped single-stranded negative-sense RNA virus in the genus Phlebovirus, family Bunyaviridae. The virus is spread by infected mosquitoes and affects ruminants and humans, causing abortion storms in pregnant ruminants, high neonatal mortality in animals, and morbidity and occasional fatalities in humans. The disease is endemic in parts of Africa and the Arabian Peninsula, but is described as emerging due to the wide range of mosquitoes that could spread the disease into non-endemic regions. Read More

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https://bmcbiotechnol.biomedcentral.com/articles/10.1186/s12
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http://dx.doi.org/10.1186/s12896-018-0489-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6290525PMC
December 2018
8 Reads

Myogenic differentiation of primary myoblasts and mesenchymal stromal cells under serum-free conditions on PCL-collagen I-nanoscaffolds.

BMC Biotechnol 2018 Nov 26;18(1):75. Epub 2018 Nov 26.

Department of Plastic and Hand Surgery and Laboratory for Tissue Engineering and Regenerative Medicine, University Hospital of Erlangen, Friedrich-Alexander University of Erlangen-Nürnberg (FAU), Krankenhausstraße 12, 91054, Erlangen, Germany.

Background: The creation of functional skeletal muscle via tissue engineering holds great promise without sacrificing healthy donor tissue. Different cell types have been investigated regarding their myogenic differentiation potential under the influence of various media supplemented with growth factors. Yet, most cell cultures include the use of animal sera, which raises safety concerns and might lead to variances in results. Read More

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http://dx.doi.org/10.1186/s12896-018-0482-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6260685PMC
November 2018
1 Read

Suitability of nutrients removal from brewery wastewater using a hydroponic technology with Typha latifolia.

BMC Biotechnol 2018 Nov 22;18(1):74. Epub 2018 Nov 22.

Department of Biotechnology, Addis Ababa Science and Technology University, Addis Ababa, Ethiopia.

Background: This study aims to assess suitability of hydroponic technology for treatment of brewery wastewater in a hydroponic bioreactor using Typha latifolia. Triplicated hydroponic bioreactor treatment units were designed, constructed and operated at a hydraulic retention time of 5 days with different surface loadings and mean hydraulic loading rate 0.023 m md. Read More

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http://dx.doi.org/10.1186/s12896-018-0484-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6251130PMC
November 2018
7 Reads

Development of luciferase-linked antibody capture assay based on luciferase immunoprecipitation systems for antibody detection of porcine reproductive and respiratory syndrome virus.

BMC Biotechnol 2018 Nov 16;18(1):73. Epub 2018 Nov 16.

Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, Shaanxi, China.

Background: Early detection of porcine reproductive and respiratory syndrome virus (PRRSV) infection of swine is necessary to control this devastating disease. By monitoring host serum antibodies to viral antigens, early virus detection within herds is feasible. In this study, recombinant antigens were generated using recombinant DNA techniques to fuse PRRSV structural protein (N) or nonstructural protein 1α (nsp1α) with the Rellina luciferase gene. Read More

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https://bmcbiotechnol.biomedcentral.com/articles/10.1186/s12
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http://dx.doi.org/10.1186/s12896-018-0483-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6240198PMC
November 2018
8 Reads
2.034 Impact Factor

Cyclodextrin enhanced the soluble expression of Bacillus clarkii γ-CGTase in Escherichia coli.

BMC Biotechnol 2018 Nov 12;18(1):72. Epub 2018 Nov 12.

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214000, China.

Background: Cyclodextrin glycosyltransferases (CGTases) catalyze the synthesis of cyclodextrins, which are circular α-(1,4)-linked glucans used in many applications in the industries related to food, pharmaceuticals, cosmetics, chemicals, and agriculture, among others. Economic use of these CGTases, particularly γ-CGTase, requires their efficient production. In this study, the effects of chemical chaperones, temperature and inducers on cell growth and the production of soluble γ-CGTase by Escherichia coli were investigated. Read More

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https://bmcbiotechnol.biomedcentral.com/articles/10.1186/s12
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http://dx.doi.org/10.1186/s12896-018-0480-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6233531PMC
November 2018
16 Reads
2.034 Impact Factor

Bioprocess optimization for pectinase production using Aspergillus niger in a submerged cultivation system.

BMC Biotechnol 2018 Nov 9;18(1):71. Epub 2018 Nov 9.

Chemistry of Natural and Microbial Products Department, National Research Centre, 12622 Dokki, Cairo, Egypt.

Background: Pectinase enzymes present a high priced category of microbial enzymes with many potential applications in various food and oil industries and an estimated market share of $ 41.4 billion by 2020.

Results: The production medium was first optimized using a statistical optimization approach to increase pectinase production. Read More

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https://bmcbiotechnol.biomedcentral.com/articles/10.1186/s12
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http://dx.doi.org/10.1186/s12896-018-0481-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6230287PMC
November 2018
9 Reads

Infectivity of adeno-associated virus serotypes in mouse testis.

BMC Biotechnol 2018 Nov 1;18(1):70. Epub 2018 Nov 1.

Institute for Stem Cell Biology and Regenerative Medicine, GKVK Campus, Bellary Road, Bengaluru, 560065, India.

Background: Recombinant adeno-associated viruses (AAVs) are emerging as favoured transgene delivery vectors for both research applications and gene therapy. In this context, a thorough investigation of the potential of various AAV serotypes to transduce specific cell types is valuable. Here, we rigorously tested the infectivity of a number of AAV serotypes in murine testis by direct testicular injection. Read More

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https://bmcbiotechnol.biomedcentral.com/articles/10.1186/s12
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http://dx.doi.org/10.1186/s12896-018-0479-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6211462PMC
November 2018
4 Reads

Efficient production of heat-stable antifungal factor through integrating statistical optimization with a two-stage temperature control strategy in Lysobacter enzymogenes OH11.

BMC Biotechnol 2018 Oct 24;18(1):69. Epub 2018 Oct 24.

Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China.

Background: Heat-stable antifungal factor (HSAF) is a newly identified broad-spectrum antifungal antibiotic from the biocontrol agent Lysobacter enzymogenes and is regarded as a potential biological pesticide, due to its novel mode of action. However, the production level of HSAF is quite low, and little research has reported on the fermentation process involved, representing huge obstacles for large-scale industrial production.

Results: Medium capacity, culture temperature, and fermentation time were identified as the most significant factors affecting the production of HSAF and employed for further optimization through statistical methods. Read More

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https://bmcbiotechnol.biomedcentral.com/articles/10.1186/s12
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http://dx.doi.org/10.1186/s12896-018-0478-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6201579PMC
October 2018
2 Reads

Genetic transformation of einkorn (Triticum monococcum L. ssp. monococcum L.), a diploid cultivated wheat species.

BMC Biotechnol 2018 Oct 23;18(1):68. Epub 2018 Oct 23.

Branch of Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry RAS, Pushchino, Moscow Region, Russian Federation.

Background: Domesticated einkorn (Triticum monococcum L.) is one of the oldest cultivated cereal crops in the world. Its small genome size (~ 5. Read More

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http://dx.doi.org/10.1186/s12896-018-0477-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6199808PMC
October 2018
1 Read

Bioconversion of duck blood cell: process optimization of hydrolytic conditions and peptide hydrolysate characterization.

BMC Biotechnol 2018 Oct 20;18(1):67. Epub 2018 Oct 20.

State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China.

Background: As the protein-laden by-product, red blood cells (RBCs) from poultry blood is a potential source of protein used as food and feed ingredient. However, RBC was currently underutilized. Therefore, it is an urgent need to develop feasible and cost-effective methods for converting poultry waste into nutritional and functional products. Read More

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http://dx.doi.org/10.1186/s12896-018-0475-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6196028PMC
October 2018
2 Reads

Targeted integration in human cells through single crossover mediated by ZFN or CRISPR/Cas9.

BMC Biotechnol 2018 Oct 19;18(1):66. Epub 2018 Oct 19.

State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510006, People's Republic of China.

Background: Targeted DNA integration is widely used in basic research and commercial applications because it eliminates positional effects on transgene expression. Targeted integration in mammalian cells is generally achieved through a double crossover event between the genome and a linear donor containing two homology arms flanking the gene of interest. However, this strategy is generally less efficient at introducing larger DNA fragments. Read More

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https://bmcbiotechnol.biomedcentral.com/articles/10.1186/s12
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http://dx.doi.org/10.1186/s12896-018-0474-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6194632PMC
October 2018
10 Reads

A highly parallel strategy for storage of digital information in living cells.

BMC Biotechnol 2018 Oct 17;18(1):64. Epub 2018 Oct 17.

Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, TX, USA.

Background: Encoding arbitrary digital information in DNA has attracted attention as a potential avenue for large scale and long term data storage. However, in order to enable DNA data storage technologies there needs to be improvements in data storage fidelity (tolerance to mutation), the facility of writing and reading the data (biases and systematic error arising from synthesis and sequencing), and overall scalability.

Results: To this end, we have developed and implemented an encoding scheme that is suitable for detecting and correcting errors that may arise during storage, writing, and reading, such as those arising from nucleotide substitutions, insertions, and deletions. Read More

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https://bmcbiotechnol.biomedcentral.com/articles/10.1186/s12
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http://dx.doi.org/10.1186/s12896-018-0476-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6191901PMC
October 2018
11 Reads

Proof of concept in utilizing in-trans surface display system of Lactobacillus plantarum as mucosal tuberculosis vaccine via oral administration in mice.

BMC Biotechnol 2018 Oct 11;18(1):63. Epub 2018 Oct 11.

Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor Darul Ehsan, Malaysia.

Background: Tuberculosis is one of the most common and deadliest infectious diseases worldwide affecting almost a third of the world's population. Although this disease is being prevented and controlled by the Bacille Calmette Guérin (BCG) vaccine, the protective efficacy is highly variable and substandard (0-80%) in adults. Therefore, novel and effective tuberculosis vaccine that can overcome the limitations from BCG vaccine need to be developed. Read More

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https://bmcbiotechnol.biomedcentral.com/articles/10.1186/s12
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http://dx.doi.org/10.1186/s12896-018-0461-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6182793PMC
October 2018
2 Reads

Rapid and efficient production of cecropin A antibacterial peptide in Escherichia coli by fusion with a self-aggregating protein.

BMC Biotechnol 2018 Oct 5;18(1):62. Epub 2018 Oct 5.

School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, China.

Background: Cecropin A (CeA), a natural cationic antimicrobial peptide, exerts potent antimicrobial activity against a broad spectrum of Gram-positive and Gram-negative bacteria, making it an attractive candidate substitute for antimicrobials. However, the low production rate and cumbersome, expensive processes required for both its recombinant and chemical synthesis have seriously hindered the exploitation and application of CeA. Here, we utilized a short β-structured self-aggregating protein, ELK16, as a fusion partner of CeA, which allowed the efficient production of high-purity CeA antibacterial peptide with a simple inexpensive process. Read More

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https://bmcbiotechnol.biomedcentral.com/articles/10.1186/s12
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http://dx.doi.org/10.1186/s12896-018-0473-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6173929PMC
October 2018
3 Reads
2.034 Impact Factor

CRISPR/Cas9-mediated genome editing induces gene knockdown by altering the pre-mRNA splicing in mice.

BMC Biotechnol 2018 Oct 3;18(1):61. Epub 2018 Oct 3.

State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China.

Background: Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR associated protein 9 (CRISPR/Cas9) has been wildly used to generate gene knockout models through inducing indels causing frame-shift. However, there are few studies concerning the post-transcript effects caused by CRISPR-mediated genome editing.

Results: In the present study, we showed that gene knockdown model also could be generated using CRISPR-mediated gene editing by disrupting the boundary of exon and intron in mice (C57BL/6 J). Read More

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http://dx.doi.org/10.1186/s12896-018-0472-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6171314PMC
October 2018
4 Reads

CRISPR/Cas9-mediated genome editing induces exon skipping by complete or stochastic altering splicing in the migratory locust.

BMC Biotechnol 2018 Sep 25;18(1):60. Epub 2018 Sep 25.

State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China.

Background: The CRISPR/Cas9 system has been widely used to generate gene knockout/knockin models by inducing frameshift mutants in cell lines and organisms. Several recent studies have reported that such mutants can lead to in-frame exon skipping in cell lines. However, there was little research about post-transcriptional effect of CRISPR-mediated gene editing in vivo. Read More

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http://dx.doi.org/10.1186/s12896-018-0465-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6156852PMC
September 2018
2 Reads

Flocculation characteristics of a bioflocculant produced by the actinomycete Streptomyces sp. hsn06 on microalgae biomass.

BMC Biotechnol 2018 Sep 21;18(1):58. Epub 2018 Sep 21.

College of Life Sciences, Henan Normal University, Xinxiang, 453007, China.

Background: Microbial flocculation is a good choice for harvest of microalgae biomass, which has gained extensive attention. There have been carried out massive studies in bacterial flocculation, many bacterial strains with flocculation activity were isolated and different types of bioflocculants were produced. However, harvest of algal biomass by bioflocculants which produced from actinomycete are deficiency. Read More

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http://dx.doi.org/10.1186/s12896-018-0471-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6151018PMC
September 2018
4 Reads
2.034 Impact Factor

Characterization of a strong and constitutive promoter from the Arabidopsis serine carboxypeptidase-like gene AtSCPL30 as a potential tool for crop transgenic breeding.

BMC Biotechnol 2018 Sep 21;18(1):59. Epub 2018 Sep 21.

The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Science, Shandong University, Jinan, Shandong, China.

Background: Transgenic technology has become an important technique for crop genetic improvement. The application of well-characterized promoters is essential for developing a vector system for efficient genetic transformation. Therefore, isolation and functional validation of more alternative constitutive promoters to the CaMV35S promoter is highly desirable. Read More

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http://dx.doi.org/10.1186/s12896-018-0470-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6151023PMC
September 2018
2 Reads
2.034 Impact Factor

Consensus design for improved thermostability of lipoxygenase from Anabaena sp. PCC 7120.

BMC Biotechnol 2018 Sep 20;18(1):57. Epub 2018 Sep 20.

State Key Laboratory of Microbial Metabolism, College of Life Science and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Rd, Shanghai, 200240, People's Republic of China.

Background: Lipoxygenase (LOX) from Anabaena sp. PCC 7120 (Ana-rLOX) offers important applications in the food industry, especially for improving aroma and dough rheological properties. However, industrial applications of LOXs have been limited by their poor thermostability. Read More

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https://bmcbiotechnol.biomedcentral.com/articles/10.1186/s12
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http://dx.doi.org/10.1186/s12896-018-0468-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6148764PMC
September 2018
2 Reads

Development of a vitrification method for preserving human myoblast cell sheets for myocardial regeneration therapy.

BMC Biotechnol 2018 Sep 10;18(1):56. Epub 2018 Sep 10.

Department of Cardiovascular Surgery, Osaka University Graduate School of Medicine, Suita, Osaka, Japan.

Background: Tissue-engineered cardiac constructs have potential in the functional recovery of heart failure; however, the preservation of these constructs is crucial for the development and widespread application of this treatment. We hypothesized that tissue-engineered skeletal myoblast (SMB) constructs may be preserved by vitrification to conserve biological function and structure.

Results: Scaffold-free cardiac cell-sheet constructs were prepared from SMBs and immersed in a vitrification solution containing ethylene glycol, sucrose, and carboxyl poly-L-lysine. Read More

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http://dx.doi.org/10.1186/s12896-018-0467-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6131806PMC
September 2018
3 Reads

A novel synthetic trivalent single chain variable fragment (tri-scFv) construction platform based on the SpyTag/SpyCatcher protein ligase system.

BMC Biotechnol 2018 Sep 10;18(1):55. Epub 2018 Sep 10.

Department of Pathology and Laboratory Medicine, College of Medicine, University of Saskatchewan, Room 2841, Royal University Hospital, 103 Hospital Drive, Saskatoon, S7N 0W8, Canada.

Background: Advances in antibody engineering provide strategies to construct recombinant antibody-like molecules with modified pharmacokinetic properties. Multermerization is one strategy that has been used to produce antibody-like molecules with two or more antigen binding sites. Multimerization enhances the functional affinity (avidity) and can be used to optimize size and pharmacokinetic properties. Read More

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http://dx.doi.org/10.1186/s12896-018-0466-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6131909PMC
September 2018
1 Read

Overexpression of a rice BAHD acyltransferase gene in switchgrass (Panicum virgatum L.) enhances saccharification.

BMC Biotechnol 2018 Sep 4;18(1):54. Epub 2018 Sep 4.

Joint BioEnergy Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.

Background: Switchgrass (Panicum virgatum L.) is a promising bioenergy feedstock because it can be grown on marginal land and produces abundant biomass. Recalcitrance of the lignocellulosic components of the switchgrass cell wall to enzymatic degradation into simple sugars impedes efficient biofuel production. Read More

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http://dx.doi.org/10.1186/s12896-018-0464-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6123914PMC
September 2018
4 Reads
2.034 Impact Factor

A rapid in vitro method to flip back the double-floxed inverted open reading frame in a plasmid.

BMC Biotechnol 2018 Aug 31;18(1):52. Epub 2018 Aug 31.

Department of Ophthalmology, Northwestern University Feinberg School of Medicine, 303 E Chicago Ave, Tarry 5-723, Chicago, IL, 60611, USA.

Background: The LoxP site based genetic switch, the FLEX, also known as DIO (Double-Floxed Inverted Open reading frame), was invented to turn on gene expression via Cre-mediated recombination. Since its first publication, numerous FLEX switch plasmids have been generated. These plasmids are designed to only work in combination with Cre. Read More

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https://bmcbiotechnol.biomedcentral.com/articles/10.1186/s12
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http://dx.doi.org/10.1186/s12896-018-0462-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6119287PMC
August 2018
10 Reads

Heterologous expression of cyclodextrin glycosyltransferase from Paenibacillus macerans in Escherichia coli and its application in 2-O-α-D-glucopyranosyl-L-ascorbic acid production.

BMC Biotechnol 2018 Aug 31;18(1):53. Epub 2018 Aug 31.

State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Puzhu South Road 30#, Nanjing, 211800, People's Republic of China.

Background: Cyclodextrin glucanotransferase (CGTase) can transform L-ascorbic acid (L-AA, vitamin C) to 2-O-α-D-glucopyranosyl-L-ascorbic acid (AA-2G), which shows diverse applications in food, cosmetic and pharmaceutical industries.

Results: In this study, the cgt gene encoding α-CGTase from Paenibacillus macerans was codon-optimized (opt-cgt) and cloned into pET-28a (+) for intracellular expression in E. coli BL21 (DE3). Read More

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http://dx.doi.org/10.1186/s12896-018-0463-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6119282PMC
August 2018
13 Reads

Oil body bound oleosin-rhFGF9 fusion protein expressed in safflower (Carthamus tinctorius L.) stimulates hair growth and wound healing in mice.

BMC Biotechnol 2018 Aug 29;18(1):51. Epub 2018 Aug 29.

College of Life Sciences, Engineering Research Center of the Chinese Ministry of Education for Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun, Jilin, 130118, China.

Background: Fibroblast growth factor 9 (FGF9) is a heparin-binding growth factor, secreted by both mesothelial and epithelial cells, which participates in hair follicle regeneration, wound healing, and bone development. A suitable source of recombinant human FGF9 (rhFGF9) is needed for research into potential clinical applications. We present that expression of oleosin-rhFGF9 fusion protein in safflower (Carthamus tinctorius L. Read More

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https://bmcbiotechnol.biomedcentral.com/articles/10.1186/s12
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http://dx.doi.org/10.1186/s12896-018-0433-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6114888PMC
August 2018
12 Reads

A paratransgenic strategy to block transmission of Xylella fastidiosa from the glassy-winged sharpshooter Homalodisca vitripennis.

BMC Biotechnol 2018 08 22;18(1):50. Epub 2018 Aug 22.

Present Address: Department of Medicine, Loyola University Stritch School of Medicine, Maywood, IL-60153, USA.

Background: Arthropod-borne diseases remain a leading cause of human morbidity and mortality and exact an enormous toll on global agriculture. The practice of insecticide-based control is fraught with issues of excessive cost, human and environmental toxicity, unwanted impact on beneficial insects and selection of resistant insects. Efforts to modulate insects to eliminate pathogen transmission have gained some traction and remain future options for disease control. Read More

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http://dx.doi.org/10.1186/s12896-018-0460-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6104007PMC
August 2018
5 Reads

Lignocellulose integration to 1G-ethanol process using filamentous fungi: fermentation prospects of edible strain of Neurospora intermedia.

BMC Biotechnol 2018 08 17;18(1):49. Epub 2018 Aug 17.

Swedish Centre for Resource Recovery, University of Borås, 50190, Borås, SE, Sweden.

Background: Integration of first- and second-generation ethanol processes is one among the alternate approaches that efficiently address the current socio-economic issues of the bioethanol sector. Edible filamentous fungus capable of utilizing pentoses from lignocelluloses and also possessing biomass application as potential animal feed component was used as the fermentation strain for the integration model. This study presents various fermentation aspects of using edible filamentous fungi in the integrated first and second generation ethanol process model. Read More

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http://dx.doi.org/10.1186/s12896-018-0444-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6098641PMC
August 2018
3 Reads

Acetosyringone treatment duration affects large T-DNA molecule transfer to rice callus.

BMC Biotechnol 2018 08 9;18(1):48. Epub 2018 Aug 9.

Department of Crop and Soil Sciences, North Carolina State University, Raleigh, NC, 27695, USA.

Background: Large T-DNA fragment transfer has long been a problem for Agrobacterium-mediated transformation. Although vector systems, such as the BIBAC series, were successfully developed for the purpose, low transformation efficiencies were consistently observed.

Results: To gain insights of this problem in monocot transformation, we investigated the T-strand accumulation of various size of T-DNA in two kinds of binary vectors (one copy vs. Read More

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http://dx.doi.org/10.1186/s12896-018-0459-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6085696PMC
August 2018
2 Reads

Expression of novel fusion antiviral proteins ricin a chain-pokeweed antiviral proteins (RTA-PAPs) in Escherichia coli and their inhibition of protein synthesis and of hepatitis B virus in vitro.

BMC Biotechnol 2018 08 6;18(1):47. Epub 2018 Aug 6.

AscentGene Inc., Gaithersburg, MD, 20878, USA.

Background: Ricin A chain (RTA) and Pokeweed antiviral proteins (PAPs) are plant-derived N-glycosidase ribosomal-inactivating proteins (RIPs) isolated from Ricinus communis and Phytolacca Americana respectively. This study was to investigate the potential production amenability and sub-toxic antiviral value of novel fusion proteins between RTA and PAPs (RTA-PAPs). In brief, RTA-Pokeweed antiviral protein isoform 1 from seeds (RTA-PAPS1) was produced in an E. Read More

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http://dx.doi.org/10.1186/s12896-018-0458-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6080542PMC
August 2018
2 Reads