410 results match your criteria Avicenna journal of medical biotechnology[Journal]


Construction, Cloning, and Expression of CagA Recombinant Protein of .

Avicenna J Med Biotechnol 2020 Apr-Jun;12(2):135-138

Department of Microbiology, Faculty of Public Health, Tehran University of Medical Sciences, Tehran, Iran.

Background: This study aimed to assess construction and expression of CagA recombinant protein of in BL21.

Methods: Bioinformatics was used in designing the desired gene by Gene Runner. Next, the construct was subcloned to pET21b vector and this process was confirmed by Polymerase Chain Reaction (PCR), enzyme digestion and sequencing techniques. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7229452PMC

Gene Expression and Levels of TGF-B in PBMC Is Associated with Severity of Symptoms in Chronic Heart Failure.

Avicenna J Med Biotechnol 2020 Apr-Jun;12(2):132-134

Research Center for Immunodeficiencies, Pediatrics Center of Excellence, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran.

Background: TGF-β1 is known to promote cardiac remodeling and fibrosis during Congestive Heart Failure (CHF). In this study, an attempt was made to investigate expression of Transforming Growth Factor beta1 (TGF-β1) and relative expansion or contraction of regulatory T-cell (Tregs) population in peripheral blood of patients with Chronic Heart Failure (CHF).

Methods: Real-time PCR assay was used to investigate expression and post-stimulation levels of TGF-β1 in cell culture supernatant of Peripheral Blood Mononuclear Cells (PBMC) of 42 patients with CHF and 42 controls. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7229453PMC

Investigation of Integron-Associated Resistance Gene Cassettes in Urinary Isolates of in Yasuj, Southwestern Iran During 2015-16.

Avicenna J Med Biotechnol 2020 Apr-Jun;12(2):124-131

Medicinal Plants Research Center, Yasuj University of Medical Sciences, Yasuj, Iran.

Background: Growing antibiotic resistance among urinary opportunistic pathogens such as has created a worrisome condition in the treatment of the Urinary Tract Infections (UTIs) in recent years. Integrons play a significant role in the dissemination of antibiotic resistance genes. The present study was conducted to investigate class 1-3 integrons and the corresponding resistance gene cassettes in urinary isolates. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7229451PMC

Optimization of Fermentation Conditions to Enhance Cytotoxic Metabolites Production by Strain RP137 from the Persian Gulf.

Avicenna J Med Biotechnol 2020 Apr-Jun;12(2):116-123

Molecular Biotechnology Laboratory, Department of Biology, Faculty of Science, Shiraz University, Shiraz, Iran.

Background: Isolation, introduction and producing bioactive compounds from bacteria, especially marine bacteria, is an attractive research area. One of the main challenges of using these metabolites as drug and their industrialization is the optimization of production conditions.

Methods: In the present study, the response surface methodology was applied to optimize the production of a cytotoxic extract (C-137-R) by () strain RP137. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7229456PMC

Analysis of Glioblastoma Multiforme Tumor Metabolites Using Multivoxel Magnetic Resonance Spectroscopy.

Avicenna J Med Biotechnol 2020 Apr-Jun;12(2):107-115

Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran.

Background: Glioblastoma Multiforme (GBM) is the most common and deadly type of primary brain tumor in adults. Magnetic Resonance Spectroscopy (MRS) is a noninvasive imaging technique used to study metabolic changes in the brain tumors. Some metabolites such as Phosphocholine, Creatine, NAA/Cr, and Pcho/Cr have been proven to show a diagnostic role in GBM. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7229458PMC

Methylation Analysis of and in Circulating Cell-Free DNA for Detection of Gastric Cancer: A Validation Study.

Avicenna J Med Biotechnol 2020 Apr-Jun;12(2):99-106

Hematology, Oncology and Stem Cell Transplantation Research Center, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran.

Background: Most of Gastric Cancer (GC) patients are diagnosed at an advanced stage with poor prognosis. Hypermethylations of several tumor suppressor genes in cell-free DNA of GC patients have been previously reported. In this study, an attempt was made to investigate the methylation status of and and their potentials for early diagnosis of GC. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7229449PMC

Design of Anti-Angiogenic Peptidomimetics and Evaluation their Biological Activity by Assays.

Avicenna J Med Biotechnol 2020 Apr-Jun;12(2):91-98

Department of Medical Biotechnology, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran.

Background: One of the important therapeutic approaches in cancer field is development of compounds which can block the initial tumor growth and the progression of tumor metastasis with no side effects. Thus, the recent study was carried out to design anti-VEGFR2-peptidomimetics as the most significant factor of angiogenesis process- and evaluate their biological activity by assays.

Methods: We designed anti-VEGFR2 peptidomimetics with anti-angiogenic activity, including compound P (lactam derivative) and compound T (indole derivative) by using in silico methods. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7229457PMC

Cysteine/Histidine-Dependent Amidohydrolase/Peptidase (CHAP)-Displayed Nano Phages: Antimicrobial Function against Methicillin-Resistant (MRSA).

Avicenna J Med Biotechnol 2020 Apr-Jun;12(2):85-90

Pediatric Infectious Diseases Research Center, Mazandaran University of Medical Sciences, Sari, Iran.

Background: Emergence and prevalence of multi drug resistance strains such as Methicillin-Resistant (MRSA) call for new antibacterial option. Endolysins as a new option is suggested. The phage display technique is suggested for production of recombinant endolysins. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7229455PMC

Polyaniline Based Electrochemical Sensor for the Detection of Dengue Virus Infection.

Avicenna J Med Biotechnol 2020 Apr-Jun;12(2):77-84

Department of Biotechnology, Faculty of Bioengineering, SRM Institute of Science and Technology, Kattankulathur, Tamil Nadu, India.

Background: Dengue burden is increasing day-by-day globally. A rapid, sensitive, cost-effective early diagnosis kit is the need of the hour. In this study, a label-free electrochemical immunosensor was proposed for dengue virus detection. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7229450PMC

Bone Regeneration Using Bio-Nanocomposite Tissue Reinforced with Bioactive Nanoparticles for Femoral Defect Applications in Medicine.

Avicenna J Med Biotechnol 2020 Apr-Jun;12(2):68-76

New Technologies Research Center, Amirkabir University of Technology, Tehran, Iran.

Background: In recent years, the method of constructing and evaluating the properties of polymer nanocomposite and bioactive ceramics in tissue engineering such as biocompatible scaffolds was studied by some researchers.

Methods: In this study, the bio-nanocomposite scaffolds of Chitosan (CS)-Hydroxyapatite (HA)-Wllastonite (WS), incorporated with 0, 10, 20 and 30 wt% of zirconium were produced using a freeze-drying method. Also, the phase structure and morphology of scaffolds were investigated using X-ray Diffraction (XRD), Scanning Electron Microscopy (SEM) and Energy Dispersive Spectroscopy (EDS). Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7229459PMC

Repositioning Drugs for Psychiatry.

Avicenna J Med Biotechnol 2020 Apr-Jun;12(2):67

Psychiatric Research Center, Roozbeh Hospital, Tehran University of Medical Sciences, Tehran, Iran.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7229454PMC

Importance of Argan Oil in Human Health According to the Dosage of Antioxidants in the Algerian Argan Fruits (.

Avicenna J Med Biotechnol 2020 Jan-Mar;12(1):66

Laboratory of Plant Ecology and Environment, Faculty of Biological Sciences, Telemcen University, Telemcen, Algeria.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7035458PMC

Evaluation of the Bioactive Potential of Secondary Metabolites Produced by a New Marine Species Isolated from the Persian Gulf.

Avicenna J Med Biotechnol 2020 Jan-Mar;12(1):61-65

Molecular Biotechnology Lab., Department of Biology, Faculty of Science, Shiraz University, Shiraz, Iran.

Background: In the present work, a newly isolated marine bacterium, sp. MP76, from coastal area of Persian Gulf around Bushehr province, Iran, was identified with the ability to produce bioactive compounds.

Methods: The pigment production was optimized by changing carbon and nitrogen sources in bacterial growth media at 28° and 220 for 5 days. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7035459PMC

Investigation of the Correlation between Androgen Receptor and ZEB1 and its Value in Progression of Gastric Cancer.

Avicenna J Med Biotechnol 2020 Jan-Mar;12(1):52-60

Hematology, Oncology and Stem Cell Transplantation Research Institute, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.

Background: Zinc-finger Enhancer Binding protein (ZEB1) acts as a transcription factor to promote cancer progression through regulating Epithelial to Mesenchymal Transition (EMT). It is well-known that ZEB1 mRNA expression is directly induced by both Estrogen Receptor (ER) and Progesterone Receptor (PR). Moreover, Androgen Receptor (AR) and PR could bind to the same regulatory element. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7035462PMC

HR9: An Important Cell Penetrating Peptide for Delivery of HCV NS3 DNA into HEK-293T Cells.

Avicenna J Med Biotechnol 2020 Jan-Mar;12(1):44-51

Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran.

Background: The delivery of exogenous genes into cells for functional expression is required for development of DNA vaccine and gene therapy in medicine and pharmacology. Cell Penetrating Peptides (CPPs) were considered to mediate gene and drug delivery into living cells. In this study, an attempt was made to evaluate the efficiency of an arginine-rich CPP, HR9, in HCV NS3 gene delivery compared to TurboFect cationic polymer and supercharged +36 GFP into HEK-293T cells. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7035460PMC

A Single Tube Overlap Extension PCR Method for Splicing of Multiple DNA Fragments.

Avicenna J Med Biotechnol 2020 Jan-Mar;12(1):37-43

Diagnostic Laboratory Sciences and Technology Research Centre, Faculty of Paramedical, Shiraz Sciences University of Medical Sciences, Shiraz, Iran.

Background: Despite the ease of conventional splicing by overlap-extension (SOEing) PCR technique in theory, when splicing more than two fragments, and especially if one of the complementary sequences is A-T rich, the attachment of the fragments would be challenging. A new rapid and highly efficient SOEing PCR assay was developed for simultaneous splicing of multiple DNA fragments and induction of site-directed mutagenesis in a single tube.

Methods: The method was adapted for splicing human beta-globin UTRs to OCT4, SOX2, KLF4, C-MYC, LIN28A, and destabilized GFP for the construction of chimeric DNA fragments for transcription. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7035467PMC

Effect of Poly-Histidine Tag Position toward Inhibition Activity of Secretory Leukocyte Protease Inhibitor as Candidate for Material Wound Healing.

Avicenna J Med Biotechnol 2020 Jan-Mar;12(1):32-36

Department of Chemistry, Faculty of Science and Technology, Universitas Airlangga, Surabaya, Indonesia.

Background: The Secretory Leukocyte Protease Inhibitors (SLPI) has many biological functions including anti-bacterial, anti-fungal, anti-viral, anti-inflammatory, and immuno-modulatory. Previous studies have shown that gene-encoding human SLPI have successfully been expressed in with a C-terminal poly-histidine tag (His-tag). The aim of this research was to investigate the inhibition activity of N-terminal His-tag position (NSLPI) and C-terminal His-tag position (CSLPI). Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7035463PMC

Expression of Human Placenta-specific 1 (PLAC1) in CHO-K1 Cells.

Avicenna J Med Biotechnol 2020 Jan-Mar;12(1):24-31

Reproductive Immunology Research Center, Avicenna Research Institute, (ACECR), Tehran, Iran.

Background: Placenta-specific 1 (PLAC1), as a new Cancer/Testis Antigen (CTA), is frequently expressed in a variety of cancers and localized to cytoplasm and plasma membrane. Surface expression of cancer target antigens is of great importance that enables antibody-mediated cancer immunotherapy. The aim of the current study was to express the intact human PLAC1 protein on plasma membrane of a eukaryotic cell as a model for future anti-PLAC1-based cancer immunotherapy. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7035464PMC

Optimization of Expansion and Activation of Human Natural Killer Cells against Breast Cancer Cell Line.

Avicenna J Med Biotechnol 2020 Jan-Mar;12(1):17-23

Department of Immunology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.

Background: Regarding to the increase of cancer deaths in recent years and disability of common therapies to eradicate cancers, as well as expansion of Natural Killer (NK) cell therapy, it seems so vital to find new useful therapies against cancers. Breast cancer is the second main cause of cancer death among women. As it is impossible for a majority of patients to receive NK cell therapy, an attempt was made to establish a low-cost and efficient method for expanding and activating NK cells against breast cancer cell line (MCF7). Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7035457PMC

Evaluation of Immune Response and Protection Induced by V-ATPase Subunit F as DNA Vaccine Against (LCED Syrian 01) After Detection and Sequencing.

Avicenna J Med Biotechnol 2020 Jan-Mar;12(1):9-16

Department of Biochemistry and Parasitology, Faculty of Pharmacy, Damascus University, Damascus, Syria.

Background: Leishmaniasis is one of the major emerging health problems worldwide and () is most prevalent in the Middle East due to conflict and environmental factors, and there is no effective prevention strategy available until now. An effective vaccine has not been developed to date. DNA vaccines are considered a promising approach to protect against this infection. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7035466PMC

AS-LAMP: A New and Alternative Method for Genotyping.

Avicenna J Med Biotechnol 2020 Jan-Mar;12(1):2-8

Student Research Committee, Thalassemia Research Center, Hemoglobinopathy Institute, Mazandaran University of Medical Sciences, Sari, Iran.

In recent decades, different methods have been introduced for the genotyping of Single Nucleotide Polymorphisms (SNPs) and mutations in nucleic acid sequences. These methods have several applications ranging from agriculture to medicine. The Loop-mediated isothermal amplification (LAMP) method was first introduced by Notomi . Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7035465PMC

The Protein Subcellular Mislocalization in Human Cancers.

Avicenna J Med Biotechnol 2020 Jan-Mar;12(1)

Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7035461PMC

Importance of Fluctuating Amino Acid Residues in Folding and Binding of Proteins.

Avicenna J Med Biotechnol 2019 Oct-Dec;11(4):339-343

Department of Bioinformatics, Faculty of Biosciences, The Marudupandiyar Institutions, Thanjavur-613403, Tamil Nadu, India.

Background: Conformational flexibility of proteins remains as one of the major events in protein-protein/DNA/ligand/small molecule binding to achieve its biological function in the cell. The availability of high-resolution structures of protein complexes is a valuable resource for researchers to understand the mechanisms behind such interactions and it is found that the flexibility of amino acid residues at binding sites is crucial for many important functions in the cell.

Methods: In this article, our statistical method (PreFRP) developed based on fluctuating amino acid residues and various amino acid indices related to flexibility/rigidity were used to study the importance of fluctuating amino acid residues in thermonucleases from pathogenic bacteria, cell penetrating peptides and intrinsically disordered proteins responsible for many neural disorders. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6925403PMC
January 2020

Integrity and Quantity Evaluation of Plasma Cell-Free DNA in Triple Negative Breast Cancer.

Avicenna J Med Biotechnol 2019 Oct-Dec;11(4):334-338

Department of Medical Genetics, Institute of Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.

Background: Triple-Negative Breast Cancer (TNBC) is a subtype of breast cancer that lacks expression of the estrogen and progesterone receptor and does not overex-press human epidermal growth factor 2 receptor protein. TNBC is associated with special characteristics, including aggressiveness, poor prognosis, and treatment response. Non-invasive blood-based molecular markers such as cell-free DNA (cfDNA) variables have been shown to be putative markers in breast cancer prognosis. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6925404PMC
January 2020

Interleukin 10 and Transforming Growth Factor Beta Polymorphisms as Risk Factors for Kawasaki Disease: A Case-Control Study and Meta-Analysis.

Avicenna J Med Biotechnol 2019 Oct-Dec;11(4):325-333

Research Center for Immunodeficiencies, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran.

Background: Alteration in serum expression of Transforming Growth Factor-beta (TGF-β) and IL-10 have been suggested to play a role in the pathogenesis of Kawasaki Disease (KD). Inconsistent reports exist on the association of IL-10 polymorphisms with KD susceptibility and Coronary Artery Aneurysms (CAA).

Methods: A number of 110 paediatric patients with KD and 140 healthy individuals were recruited to investigate the frequency of Single Nucleotide Polymorphisms (SNPs) of TGF-β C/T at codon 10 (rs1982073), C/G at codon 25 (rs1800471) and IL-10 A/G at -1082 (rs1800896), C/T at -819 (rs1800871) and A/C at -592 (rs1800872) and their respective genotype and haplotypes. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6925396PMC
January 2020

Effect of Sodium Butyrate on mRNA Expression as a Transcription Factor of HDAC8 in Human Colorectal Cancer Cell Lines.

Avicenna J Med Biotechnol 2019 Oct-Dec;11(4):317-324

Department of Molecular Medicine, Pasteur Institute of Iran, Tehran, Iran.

Background: LHX1 is an important transcription factor for the gene. The aim of this study was to investigate the effect of Sodium Butyrate (SB), as a histone deacetylase inhibitor, on the expression of gene in colorectal cancer cell lines.

Methods: HT-29 and HCT-116 cell lines were treated with 6. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6925397PMC
January 2020

Menstrual Blood Stem Cell Transplantation in Mice Model of Acute Liver Failure: Does Gender of Recipient Affect the Outcome?

Avicenna J Med Biotechnol 2019 Oct-Dec;11(4):308-316

Department of Molecular Medicine, Faculty of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.

Background: There exists a dramatic rise in liver failure and numerous patients undergo liver transplant for life-saving reasons annually. Introducing alternatives to allo-graft transplantation is necessary due to present limitations. Recently, a noninvasive stem cell population from Menstrual blood-derived Stem Cells (MenSCs) has been identified. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6925399PMC
January 2020

A Metabolomic Study to Identify Potential Tissue Biomarkers for Indomethacin-Induced Gastric Ulcer in Rats.

Avicenna J Med Biotechnol 2019 Oct-Dec;11(4):299-307

Department of Basic Sciences, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Background: Gastric Ulcer (GU) is the most prevalent gastrointestinal disorder induced by various factors and Non-Steroid Anti-Inflammatory Drugs (NSAIDs) as one of the most common reasons. Due to the absence of appropriate molecular markers for GU, the aim of this study was to utilize a metabolomics approach in order to find potential metabolite markers for the disease.

Methods: Stomach tissue samples from indomethacin-treated rats and normal controls were used to perform a 1H-NMR metabolomics study. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6925398PMC
January 2020

G2 Dendrimer as a Carrier Can Enhance Immune Responses Against HCV-NS3 Protein in BALB/c Mice.

Avicenna J Med Biotechnol 2019 Oct-Dec;11(4):292-298

Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran.

Background: Hepatitis C virus (HCV) infection is a major issue of public health. It seems of paramount importance to find an effective vaccine against HCV infection. The best vaccine candidate should induce robust cellular responses. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6925401PMC
January 2020

Anti-Influenza Virus Activity and Phenolic Content of Pomegranate (Punica granatum L.) Peel Extract and Fractions.

Avicenna J Med Biotechnol 2019 Oct-Dec;11(4):285-291

Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran.

Background: Influenza virus, associated with high level of morbidity and mortality, has been recently considered a public health concern while the choices for the control and treatment of the disease are limited. The present study was conducted to evaluate activity of pomegranate peel extract and its fractions against virus .

Methods: In this research, ethyl alcohol extract of pomegranate peel was prepared and subjected to fractionation with different polarities. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6925405PMC
January 2020

Design and Fabrication of a Novel Microfluidic System for Enrichment of Circulating Tumor Cells with the Assistance of Computer Simulations.

Avicenna J Med Biotechnol 2019 Oct-Dec;11(4):277-284

Department of Chemical Engineering, Sharif University of Technology, Tehran, Iran.

Background: Cancer is the first cause of death in developed countries. The heterogeneous nature of cancer requires patient-specified treatment plans. One reliable approach is collecting Circulating Tumour Cells (CTCs) and using them for prognosis and drug response assessment purposes. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6925400PMC
January 2020

Sortilin as a Novel Diagnostic and Therapeutic Biomarker in Chronic Lymphocytic Leukemia.

Avicenna J Med Biotechnol 2019 Oct-Dec;11(4):270-276

Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.

Background: The overexpression of sortilin/neurotensin receptor 3 has previously been reported in various human solid tumors but not in hematological malignancies. Here, we report the overexpression of sortilin in leukemic cells from patients with Chronic Lymphocytic Leukemia (CLL).

Methods: Flow cytometry was used to compare the expression of sortilin in CLL patients (n=52) and healthy individuals (n=26). Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6925395PMC
January 2020

Microbiome and Schizophrenia.

Avicenna J Med Biotechnol 2019 Oct-Dec;11(4):269

Psychiatric Research Center, Roozbeh Hospital, Tehran University of Medical Sciences, Tehran, Iran.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6925402PMC
January 2020

Cloning and Expression of Gene in as a Food Grade Vaccine.

Avicenna J Med Biotechnol 2019 Jul-Sep;11(3):264-267

Razi Vaccine and Serum Research Institute, Agricultural Research, Education & Extension Organization, Karaj, Iran.

Background: Brucellosis is still an important health problem in under developing countries and researches for finding efficient vaccine are going on. gene is a good candidate for brucellosis vaccine and investigations showed that ( with several positive characteristic are attractive for protein expression as a live delivery vectors. These fast growing bacteria need no aeration, are easy to handle, have no exotoxin, endotoxin and protease, so the cost of culturing is inexpensive. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6626509PMC

Pre-validation of Gene Editing by CRISPR/Cas9 Ribonucleoprotein.

Avicenna J Med Biotechnol 2019 Jul-Sep;11(3):259-263

Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.

Background: The CRISPR/Cas9 genome editing system is a powerful and simple gene editing method. The format of the CRISPR components is one of the important factors in targeting efficiency. Compared to plasmid or mRNA (IVTs) format, using the CRISPR/Cas9 system as Cas9-crRNA-tracrRNA RNP format is more efficient and rapid, especially in minimizing some of the pitfalls of CRISPR-mediated gene editing. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6626505PMC

Whole Exome Sequencing of an X-linked Thrombocytopenia Patient with Normal Sized Platelets.

Avicenna J Med Biotechnol 2019 Jul-Sep;11(3):253-258

Department of Medical Biotechnology, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran.

Wiskott-Aldrich Syndrome () is a rare X-linked recessive Primary Immunodeficiency (PID) caused by mutations in gene which encodes a protein known as WASp. WASp plays important roles in cytoskeletal functions that compromise multiple aspects of normal cellular activity including proliferation, phagocytosis, immune synapse formation, adhesion and directed migration. WASp defect particularly causes platelets abnormality which is presented in forms of decrease of Mean Platelet Volume (MPV) and thrombocytopenia in most conditions; nevertheless, some studies reported patients with a normal or large size of platelets in recent years. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6626504PMC
August 2019
3 Reads

Sericin Ameliorates the Capacitation State and Chromatin Integrity of Frozen-Thawed Stallion Spermatozoa by Reducing Oxidative Stress.

Avicenna J Med Biotechnol 2019 Jul-Sep;11(3):245-252

Research Institute of Animal Embryo Technology, Shahrekord University, Shahrekord, Iran.

Background: In the process of sperm cryopreservation, apart from cryoinjury, the production of Reactive Oxygen Species (ROS) can adversely affect the integrity of chromatin and cellular membranes. Addition of natural antioxidants to freezing medium is an approach to reduce the destructive effects of ROS on sperm.

Methods: In this study, during 60 of cooling process, the ejaculates of five stallions were diluted in the following media: INRA 82 medium as Control (C), INRA 82 medium supplemented with 0. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6626508PMC
August 2019
1 Read

Investigation of the Association between 5-Hydroxytryptamine Transporter Gene-Linked Polymorphic Region with Type 2 Diabetes Mellitus, Obesity and Biochemical Profiles of Serum in Iranian Population.

Avicenna J Med Biotechnol 2019 Jul-Sep;11(3):239-244

Noncommunicable Diseases Research Center, Fasa University of Medical Sciences, Fasa, Iran.

Background: Type 2 Diabetes Mellitus (T2DM) is a serious problem in the world. 5-Hydroxytryptamine (5-HT, serotonin) plays an important role in obesity, glucose control and insulin resistance. The polymorphism of the serotonin transporter gene linked promoter region (5-HTTLPR) might influence 5-HTT expression and serotonin uptake. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6626511PMC
August 2019
2 Reads

Effect of Hydroalcoholic Ginger Extract on Brain HMG-CoA Reductase and CYP46A1 Levels in Streptozotocin-induced Diabetic Rats.

Avicenna J Med Biotechnol 2019 Jul-Sep;11(3):234-238

Department of Biochemistry, Cellular and Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

Background: Patients with diabetes present with lipid disorders, including hypercholesterolemia, which can be a high-risk factor for atherosclerosis. Recently, increasing interest has been focused on anti-lipidemic function of herbal medicines, especially (known as ginger), in diabetes. However, the mechanism underlying the effect of ginger on some players involved in cholesterol homeostasis of Central Nervous System (CNS) among diabetic patients remains unclear. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6626506PMC
August 2019
3 Reads

Effect of Recombinant Helicobacter Outer Membrane Protein H (HopH) on Nitric Oxide Production by Peripheral Macrophage in BALB/c Mice.

Avicenna J Med Biotechnol 2019 Jul-Sep;11(3):229-233

Department of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.

Background: Some products of bacteria are reported as an immunomodulator. The () outer membrane proteins play an important role in stimulation of immune system. The present study was performed to determine the effect of recombinant HopH of on Nitric Oxide (NO) production and viability of mouse peritoneal macrophages. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6626510PMC
August 2019
1 Read

Phylogenetic Analysis of Virus among Household Members with HBV Chronic Infection.

Avicenna J Med Biotechnol 2019 Jul-Sep;11(3):221-228

Department of Microbiology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Background: Intrafamilial spread of virus (HBV) infection in Iran has only been investigated with serological testing without using molecular studies as the most informative and definitive type of analysis.

Methods: In the present study, intrafamilial transmission of HBV among family members of Iranian index HBsAg carriers was investigated using phylogenetic analysis of the S region of the viral genome. Nested polymerase chain reaction was used for detection of HBV DNA in serum samples from 22 index and 43 contact patients with chronic HBV infection. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6626507PMC
August 2019
2 Reads

MIG1 Glucose Repression in Metabolic Processes of Genetics to Metabolic Engineering.

Avicenna J Med Biotechnol 2019 Jul-Sep;11(3):215-220

Institute of Chemical Biology, Faculty of Natural Sciences and Engineering, Ilia State University, Tbilisi, Georgia.

Background: Although has several industrial applications, there are still fundamental problems associated with sequential use of carbon sources. As such, glucose repression effect can direct metabolism of yeast to preferably anaerobic conditions. This leads to higher ethanol production and less efficient production of recombinant products. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6626512PMC
August 2019
2 Reads

The p53 Modulated Cytotoxicity of Polysaccharide Against Resistance Ovarian Cancer Cells.

Avicenna J Med Biotechnol 2019 Jul-Sep;11(3):208-214

Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran.

Background: Marine environment is a valuable source of bioactive compounds with variable medicinal properties. Previously, it was shown that extracted polysaccharide has prominent cytotoxic effect on HeLa human cervical cancer cells. In the present study, the anti-cancer properties of polysaccharide extracted from ( were examined in comparison with paclitaxel as a conventional drug against resistant ovarian cancer; also, its related mechanism against A2780cp ovarian cancer cells was investigated. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6626503PMC
August 2019
2 Reads

Depression and Inflammation: Is There any Role for Biomarkers?

Avicenna J Med Biotechnol 2019 Jul-Sep;11(3):207

Psychiatric Research Center, Roozbeh Hospital, Tehran University of Medical Sciences, Tehran, Iran.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6626502PMC
August 2019
1 Read

Evaluation of the Effect of Morphine and Imiquimodon Expression of and from Lesion RNA Extracted from BALB/c Mice Infected with .

Avicenna J Med Biotechnol 2019 Apr-Jun;11(2):202-205

Blood Transfusion Research Center, High Institute for Education and Research in Transfusion Medicine, Tehran, Iran.

Background: Toll-Like Receptors (TLRs) are the cause of phagocytosis activation and destruction of the infection agents. In addition, new evidences support the idea that TLRs play a vital role in starting the acquired immunity reactions.

Methods: In this study, it has been attempted to infect the BALB/c mice with Leishania ajor () and treat them using morphine and imiquimod; then the expressions of ,4 from treated lesion were studied by using Real-Time PCR method. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6490411PMC
May 2019
4 Reads

Antibiotic Susceptibility Patterns and Prevalence of Some Extended Spectrum Beta-Lactamases Genes in Gram-Negative Bacteria Isolated from Patients Infected with Urinary Tract Infections in Al-Najaf City, Iraq.

Avicenna J Med Biotechnol 2019 Apr-Jun;11(2):192-201

Department of Microbiology, Faculty of Science, University of Kufa, Kufa, Iraq.

Background: Urinary Tract Infection (UTI) in patients with Chronic Kidney Disease (CKD) caused by multi-drug resistance and Extended Spectrum Beta Lactamase (ESBL)-producing gram-negative bacteria has been increased in different countries. The aim of the present study was to detect the antibiotic susceptibility patterns and the distribution of , and genes in gram-negative bacteria isolated from outpatients infected with UTI, with and without CKD in Al-Najaf city, Iraq.

Methods: A total of 120 non-duplicate urine samples were collected from outpatients (37 male and 83 female) infected with UTI in Al-Najaf city, Iraq; 60 samples from patients Without Kidney Disease (WKD) and 60 samples from patients with CKD. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6490404PMC
May 2019
8 Reads

Single Nucleotide Polymorphism of Gene and Susceptibility to Rheumatoid Arthritis in Iranian Population.

Avicenna J Med Biotechnol 2019 Apr-Jun;11(2):187-191

Rheumatology Research Center, Tehran University of Medical Sciences, Tehran, Iran.

Background: Rheumatoid Arthritis (RA) is a debilitating disorder in which the immune system mainly targets the synovial tissue. Janus kinase family including tyrosine kinase 2 (TYK2) is one of the crucial mediators of the downstream signaling pathway of inflammatory cytokines that further contributes to RA pathogenesis. In this study, the association of gene rs34536443 polymorphism, which may affect the function of TYK protein and, hence, the inflammatory settings, with RA susceptibility was investigated. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6490405PMC
May 2019
7 Reads

Immunogenicity of Cork and Loop Domains of Recombinant Utilization Protein in Murine Model.

Avicenna J Med Biotechnol 2019 Apr-Jun;11(2):180-186

Departeman of Biology, Science and Art University, Yazd, Iran.

Background: is a bothersome fatal pathogen, particularly in healthcare system. Persistence and successful invasion of in vertebrate host cells largely depends on iron acquisition methods. Siderophore molecules and Iron-Regulated Outer Membrane Proteins (IROMPs) are the two essential members of iron acquisition system. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6490406PMC
May 2019
3 Reads

Bacteriostatic Potency of FeO Against in Synergy with Antibiotics by DDST Method.

Avicenna J Med Biotechnol 2019 Apr-Jun;11(2):176-179

Department of Biology, Tehran North Branch, Islamic Azad University, Tehran, Iran.

Background: In this study, bacteriostatic potency of the Iron oxide nanoparticles against ( (a clinical sample and the ATCC11700 strain) was investigated.

Methods: Nanoparticles' bacteriostatic concentration was determined and used to appraise the characteristics of the Iron Oxide (FeO) against the isolates. Antimicrobial examinations with 10 were performed at the baseline. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6490414PMC
May 2019
13 Reads

Development of Sensitive and Rapid RNA Transcription-based Isothermal Amplification Method for Detection of .

Avicenna J Med Biotechnol 2019 Apr-Jun;11(2):169-175

Department of Medical Biotechnology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.

Background: The accurate and early diagnosis of tuberculosis is important for its effective management. During the last decade, several molecular methods for detection of Tuberculosis (TB) have been developed. Since RNA especially mRNA has a generally much shorter half-life than DNA, its detection may be useful for the assessment of viability of bacteria. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6490409PMC
May 2019
11 Reads