355 results match your criteria Avicenna journal of medical biotechnology[Journal]


The Association of PON1 192 Q/R Polymorphism with the Risk of Idiopathic Male Infertility in Northern Iran.

Avicenna J Med Biotechnol 2019 Jan-Mar;11(1):127

[This retracts the article on p. 253 in vol. 10, PMID: 30555659. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6359700PMC
February 2019
2 Reads

Triploidy and Routine Combined First Trimester Pregnancy Screening.

Avicenna J Med Biotechnol 2019 Jan-Mar;11(1):124-126

Genetics Research Center, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran.

Background: This report is about a pregnancy with a triploid fetus and underscores the potential of first trimester combined screening to detect this devastating chromosomal aberration earlier in pregnancy. This report is about a pregnancy with a triploid fetus identified from the first trimester combined screening and confirmed by amniocentesis.

Methods: A 28 year old, G5P2AB2 woman was referred to our clinic at 15 weeks of gestation due to a remarkable decrease of her first trimester double biochemical markers and therefore in the high-risk range for trisomy 13 and 18. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6359692PMC
February 2019
1 Read

Development of One-Step Tetra-primer ARMS-PCR for Simultaneous Detection of the I/D and Gene Polymorphisms and the Correlation with CAD Patients.

Avicenna J Med Biotechnol 2019 Jan-Mar;11(1):118-123

Research Center of Prevention and Epidemiology of Non-Communicable Disease, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.

Background: The Insertion/Deletion and gene polymorphisms could be associated with pathogenesis of essential hypertension and cardiovascular disorders and Coronary Artery Disease (CAD). In the present study, a fast and novel approach of multiplex Tetra-Primer Amplification Refractory Mutation System-PCR (T-ARMS-PCR) was developed for simultaneous detection of two SNPs including (rs4340) and 2350A>G (rs4343) of () gene.

Methods: The present research was performed using 148 blood samples taken from patients with CAD and 135 healthy individuals. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6359703PMC
February 2019

Enhancing Stability of Destabilized Green Fluorescent Protein Using Chimeric mRNA Containing Human Beta-Globin 5' and 3' Untranslated Regions.

Avicenna J Med Biotechnol 2019 Jan-Mar;11(1):112-117

Diagnostic Laboratory Sciences and Technology Research Center, Faculty of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran.

Background: In spite of recent progress in mRNA technologies and their potential applications for treatment of human diseases, problems such as the transient nature of mRNA limit the stability of gene up-regulation and, thus, potentially reduce mRNA efficiency for gene therapy. Using human β-globin 5' and 3' untranslated regions (UTRs), this study aimed to develop the different chimeric constructs of mRNAs to increase the stability of destabilized green fluorescent protein (EGFPd2) in HEK 293 cells.

Methods: Purified human β-globin (HBG) 5'-3'UTRs, and the coding sequence of destabilized green fluorescent protein (EGFPd2) were amplified separately and ligated to each other using SOEing PCR method in a different format. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6359690PMC
February 2019

Prediction of RNA- and DNA-Binding Proteins Using Various Machine Learning Classifiers.

Avicenna J Med Biotechnol 2019 Jan-Mar;11(1):104-111

Department of Biophysics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.

Background: Nucleic acid-binding proteins play major roles in different biological processes, such as transcription, splicing and translation. Therefore, the nucleic acid-binding function prediction of proteins is a step toward full functional annotation of proteins. The aim of our research was the improvement of nucleic-acid binding function prediction. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6359699PMC
February 2019

Identification of Potential Lead Molecules for Zika Envelope Protein from In Silico Perspective.

Avicenna J Med Biotechnol 2019 Jan-Mar;11(1):94-103

Faculty of Biotechnology and Bioinformatics, D.Y. Patil Deemed to be University, CBD Belapur, Navi Mumbai, India.

Background: Zika virus is the family member of flavivirus with no reported clinically approved drugs or vaccines in the market till date. This virus is spread by mosquitoes, and can also be transmitted through sexual contact or blood transfusions. There are reported medical conditions like microcephaly among new-borns delivered by infected pregnant women. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6359704PMC
February 2019
1 Read

Expression and Activity of Platelet Endothelial Nitric Oxide Synthase Are Decreased in Patients with Coronary Thrombosis and Stenosis.

Avicenna J Med Biotechnol 2019 Jan-Mar;11(1):88-93

Department of Biochemistry, Faculty of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.

Background: Nitric Oxide (NO) which is synthesized by endothelial Nitric Oxide Synthase (eNOS) in both vascular tissues and platelets plays an important role as a protective mediator in the cardiovascular system. It modulates blood pressure, vasodilation and thrombosis. In this regard, eNOS activity and gene expression in platelets and NO levels in patients' plasmas with Coronary Thrombosis (CT) and stenosis diseases were determined. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6359691PMC
February 2019

Development and Comparison of In-house Line Probe Assay (LiPA) and SYBR Green Real-time PCR Regarding the Detection of Periodontal Pathogens.

Avicenna J Med Biotechnol 2019 Jan-Mar;11(1):80-87

Department of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran.

Background: Periodontal disease, which can become a chronic condition, is an inflammatory disease that upsets the soft and hard structures supporting the teeth. The aim of the present study was to design and develop an in-house Line Probe Assay (LiPA), to detect putative periodontitis-related bacterial pathogens, and compare it with SYBR Green Real-time PCR.

Methods: The LiPA method was launched using biotinylated 16s rRNA universal primers and specific probes for each of the five bacteria including , , , and . Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6359689PMC
February 2019

Characterization of Monoclonal and Polyclonal Antibodies Recognizing Prostate Specific Antigen: Implication for Design of a Sandwich ELISA.

Avicenna J Med Biotechnol 2019 Jan-Mar;11(1):72-79

Department of Immunology, Faculty of Public Health, Tehran University of Medical Sciences, Tehran, Iran.

Background: Prostate cancer is the second most common cancer in men. Prostate-Specific Antigen (PSA) is a tumor-associated glycoprotein with enzymatic activity which is secreted by the prostate gland. Following entry to the blood, 70-90% of PSA forms complexes with protease inhibitors and its enzymatic activity is inhibited. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6359702PMC
February 2019

The Effect of Gene of Human T-cell Leukemia Virus Type-1 on the Expression of CCR5 in K562 Cell Line.

Avicenna J Med Biotechnol 2019 Jan-Mar;11(1):67-71

Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran.

Background: Tax-1 protein of Human T-cell Leukemia Virus type 1(HTLV-1) serves as a key transcriptional regulatory gene product and has a crucial role in transactivating genes of infected cells by employing their transcriptional factors. This modulation includes induction of genes which encode CC-chemokines and their receptors. In this study, a recombinant vector containing Tax-1 gene was made and tested for its ability to induce CCR5 (CC chemokine receptor 5) expression in K562 cell line. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6359701PMC
February 2019

Statistical Optimization of Process Parameters by Central Composite Design (CCD) for an Enhanced Production of L-asparaginase by BSH-3, a Novel Species.

Avicenna J Med Biotechnol 2019 Jan-Mar;11(1):59-66

Department of Biotechnology, GITAM Institute of Science, GITAM University, Visakhapatnam- 530 045, Andhra Pradesh, India.

Background: The present study focused on the production of L-asparaginase using Solid State Fermentation (SSF) by

Methods: Initially, five significant parameters (Carbon source; Nitrogen source, temperature, pH and incubation period) were identified that affect the production process of L-asparaginase using Classical One Factor at a Time (OFAT) optimization. An optimized L-asparaginase specific activity obtained by OFAT was recorded as 85.7 . Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6359705PMC
February 2019

A Multidisciplinary Study to Evaluate the Anti-quorum Sensing Ability of Phyto-compounds in Jacq.

Avicenna J Med Biotechnol 2019 Jan-Mar;11(1):48-58

PG and Research Department of Botany, Arignar Anna Government College, Thiruvalluvar University, Villupuram, Tamilnadu, India.

Background: () causing numerous diseases in humans, have become resistant to antibiotics, hence, urging the need for alternative medicines.

Methods: In this study, the Indian medicinal weed, ) extracted and fractioned through column chromatography was subjected to antibacterial and anti-quorum sensing activity against and Methicillin Resistant Staphylococcus aureus (MRSA).

Results: The obtained results confirmed fraction F44 to have significant effect as antimicrobial and anti-biofilm agent against both the micro-organism. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6359695PMC
February 2019

The Anti-cancer Effects of Hydroalcoholic Extract.

Avicenna J Med Biotechnol 2019 Jan-Mar;11(1):43-47

Neuroscience Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.

Background: Recently, due to the steep increase in cancer lethality statistics, pharmaceutical societies seek approaches for designing drugs with higher efficiency and lower expenses. Plant-based drugs have therefore gained much attention, due to their abundance and ease of accessibility, and their higher effectiveness.

Methods: Wild-grown caper [ ()] was collected from northern Iran and next 100 of the powder was added to 300 of a solvent (Ethanol 80), the solution was mixed for 72 and later filtered Whatman filter papers. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6359694PMC
February 2019

Preconditioning with SDF-1 Improves Therapeutic Outcomes of Bone marrow-derived Mesenchymal Stromal Cells in a Mouse Model of STZ-induced Diabetes.

Avicenna J Med Biotechnol 2019 Jan-Mar;11(1):35-42

Department of Anatomical Sciences, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.

Background: Nowadays, transplantation of Bone marrow-derived Mesenchymal Stromal Cells (BMSCs) is currently an important alternative therapy for patient's type 1 diabetes mellitus. But a number of critical obstacles lie ahead of this new strategy including reducing stem cell homing to the damaged tissue due to oxidative stress. The purpose of the present study was to investigate whether preconditioning of BMSCs with SDF-1 could enhance their homing to the pancreas and promote regeneration of the pancreatic β cells after being intravenously injected. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6359696PMC
February 2019
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Evaluation of Differential Gene Expression during Transdifferentiation of Bone Marrow Stromal Cells to Glial Phenotype in the Presence of Cerebrospinal Fluid.

Avicenna J Med Biotechnol 2019 Jan-Mar;11(1):28-34

Department of Anatomy & Cell Biology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.

Background: The present study assessed the alteration of gene expression during transdifferentiation of Bone Marrow Stromal Cells (BMSCs) into oligodendrocyte in the presence of Cerebrospinal Fluid (CSF).

Methods: BMSCs were collected from female Sprague-Dawley rats and were cultured in DMEM/F12 medium supplemented with Retinoic Acid (RA), basic Fibroblast Growth Factor (bFGF), and Epidermal Growth Factor (EGF). CSF was added daily to the culture media. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6359706PMC
February 2019

Recovery of MicroRNA from Stored Bone Marrow Aspirate Slides.

Avicenna J Med Biotechnol 2019 Jan-Mar;11(1):24-27

Department of Biology, Faculty of Science, University of Isfahan, Isfahan, Iran.

Background: Archived bone marrow aspirate slides are almost infinite, readily available resource of biospecimens that enable retrospective molecular investigations of diseases. RNAs obtained from slides has limitations in utility because of their low quality and highly fragmented nature. MicroRNAs are small (<22 nt) noncoding RNAs with various cellular regulatory roles. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6359693PMC
February 2019
1 Read

Antibody-Drug Conjugates: Possibilities and Challenges.

Avicenna J Med Biotechnol 2019 Jan-Mar;11(1):3-23

Department of Immunology, Faculty of Public Health, Tehran University of Medical Sciences, Tehran, Iran.

The design of Antibody Drug Conjugates (ADCs) as efficient targeting agents for tumor cell is still in its infancy for clinical applications. This approach incorporates the antibody specificity and cell killing activity of chemically conjugated cytotoxic agents. Antibody in ADC structure acts as a targeting agent and a nanoscale carrier to deliver a therapeutic dose of cytotoxic cargo into desired tumor cells. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6359697PMC
February 2019
1 Read

Monoclonal Antibody a Promising Treatment for Depression.

Avicenna J Med Biotechnol 2019 Jan-Mar;11(1):1-2

Psychiatric Research Center, Roozbeh Hospital, Tehran University of Medical Sciences, Tehran, Iran.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6359698PMC
February 2019

Arylamine N-acetyltransferase 2 Polymorphisms and the Risk of Endometriosis.

Avicenna J Med Biotechnol 2018 Oct-Dec;10(4):277-278

Academic Unit of Obstetrics and Gynaecology IRCCS Ospedale Policlinico San Martino, University of Genoa Largo Rosanna Benzi 10, 16121 Genova, Italy.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6252036PMC
December 2018

Gene c.2860C>T Mutation in CFEOM1A: The First Report from Iran.

Avicenna J Med Biotechnol 2018 Oct-Dec;10(4):273-276

Genome Research Division, Department of Human Genetics, Radboud University Medical Center, Geert Grooteplein Zuid 10, 6525 KL, Nijmegen, The Netherlands.

Congenital Fibrosis of the Extra Ocular Muscles1 (CFEOM1) is an autosomal dominant condition, caused by mutation in the and . It is characterized by congenital non-progressive restrictive ophthalmoplegia and ptosis. Mutational analysis of the known genes in such rare diseases by Sanger sequencing not only prevents wasting the time and expenses but also speeds diagnosis process, genetic counseling, and the possibility of prenatal diagnosis. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6252030PMC
December 2018
10 Reads

Rapid and Simple Detection of by Loop-Mediated Isothermal Amplification Assay in Urine Specimens.

Avicenna J Med Biotechnol 2018 Oct-Dec;10(4):269-272

Department of Microbiology, Faculty of Biological Sciences, Shahid Beheshti University, Tehran, Iran.

Background: To improve urinary tract infection detection, we evaluated the specificity and sensitivity of Loop-mediated isothermal Amplification Method (LAMP) for detection of the in urine samples, for the first time.

Methods: Primers were designed to target the malB gene of . LAMP assay was performed on urine specimens collected from patients with urinary tract infection symptoms. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6252025PMC
December 2018
1 Read

A Study of Recombinant Factor IX in Drosophila Insect S2 Cell Lines Through Transient Gene Expression Technology.

Avicenna J Med Biotechnol 2018 Oct-Dec;10(4):265-268

Department of Biotechnology, Sabzevar Branch, Islamic Azad University, Sabzevar, Iran.

Background: Since the mass production of recombinant proteins requires the development of stable cell lines which is a time-consuming complex process, the use of transient expression on a large scale can be a comparatively useful alternative. Although various cell lines have been used for the expression of recombinant proteins, only a limited number of cells enjoy a high transfection characteristic and the ability to adapt to serum-free suspension culture easily. In the present study, the S2 cells from Drosophila insect with the ability to grow in suspension and serum-free cultures were used for the expression of factor IX (FIX) using Transient Gene Expression (TGE) technique. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6252033PMC
December 2018
1 Read

A Study on the Association between Mutation and HCV Infection in Iranian Patients.

Avicenna J Med Biotechnol 2018 Oct-Dec;10(4):261-264

Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran.

Background: Mutations in the coding region of the Chemokine Receptor 5 (5) genes reduce or eliminate expression in immune cells and progression of HCV infection. This study aimed to investigate the role of this mutation in HCV infection in Iranian patients in comparison with healthy individuals.

Methods: 100 HCV infected patients and 100 healthy individuals were randomly selected. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6252026PMC
December 2018
12 Reads

Pattern Analysis of Short Tandem Repeats Allele Frequencies among the Population of Khuzestan Province, South of Iran.

Avicenna J Med Biotechnol 2018 Oct-Dec;10(4):257-260

Department of Forensic Medicine, Khuzestan Legal Medicine Organization, Ahvaz, Iran.

Background: The basis of genetic fingerprinting and DNA profiling in forensic laboratories is the use of Short Tandem Repeats (STRs) according to local and ethnical genetics characteristics.

Methods: Forensic parameters and allele frequencies for 15 autosomal STRs in 100 unrelated individuals from Khuzestan province, south Iran were determined. PCR was carried out for amplification of STRs and GeneMapper ID software was used for genotyping and allelic analyzing. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6252038PMC
December 2018

The Association of PON1 192 Q/R Polymorphism with the Risk of Idiopathic Male Infertility in Northern Iran.

Avicenna J Med Biotechnol 2018 Oct-Dec;10(4):253-256

Cellular and Molecular Research Center, Faculty of Medical Sciences, Guilan University of Medical Sciences, Rasht, Iran.

Background: Infertility is defined as the inability to achieve pregnancy after 12 months of regular unprotected sexual intercourse. Environmental and genetic factors are involved in male infertility. The polymorphism studies have a crucial role in disease recognition. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6252028PMC
December 2018

Interaction Effect of RsaI and BamHI Polymorphisms of TGFα, BMP2 and BMP4 on the Occurrence of Non-Syndromic Cleft Lip and Palate in Iranian Patients.

Avicenna J Med Biotechnol 2018 Oct-Dec;10(4):248-252

Dentofacial Deformities Research Center, Research Institute of Dental Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Background: Orofacial cleft is the most common congenital defect of the maxillofacial region. Its non-syndromic type is multi-factorial, and several genes are involved in its occurrence. This study aimed to assess the interaction effect of Rsal and BamHI polymorphisms of gene and Bone Morphogenetic Protein-2 (BMP2) and BMP4 variants on the occurrence of Non-Syndromic Cleft Lip and Palate (NSCLP) in the Iranian population. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6252027PMC
December 2018
4 Reads

Association of G/C (rs638405) Polymorphism in Gene with Alzheimer's Disease.

Avicenna J Med Biotechnol 2018 Oct-Dec;10(4):242-247

Department of Biochemistry, Faculty of Medical, Cellular and Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

Background: Alzheimer's Disease (AD) is a neurodegenerative disorder, which is the most common cause of dementia in the elderly. Accumulation of β-amyloid plaques outside neurons is the most important pathological hallmark of AD, which is produced by cleavage of amyloid precursor protein by the Alzheimer's β-secretase (). Since is a key enzyme in the formation of β-amyloid peptides, the purpose of this study was to assess the association between polymorphisms of G/C (rs638405) gene with sporadic AD in Khuzestan, Isfahan and Fars provinces in Iran. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6252032PMC
December 2018
1 Read

Exploring Potential Biomarkers Underlying Pathogenesis of Alzheimer's Disease by Differential Co-expression Analysis.

Avicenna J Med Biotechnol 2018 Oct-Dec;10(4):233-241

Research Center for Molecular Medicine, Hamedan University of Medical Sciences, Hamedan, Iran.

Background: Alzheimer's Disease (AD) is the most common form of dementia in the elderly. Due to the facts that biological causes of AD are complex in addition to increasing rates of AD worldwide, a deeper understanding of AD etiology is required for AD treatment and diagnosis.

Methods: To identify molecular pathological alterations in AD brains, GSE36980 series containing microarray data samples from temporal cortex, frontal cortex and hippocampus were downloaded from Gene Expression Omnibus (GEO) database and valid gene symbols were subjected to building a gene co-expression network by a bioinformatics tool known as differential regulation from differential co-expression (DCGL) software package. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6252023PMC
December 2018

HER-3 Knocking Down Induces G2/M Arrest in Gastric Cancer Cells.

Avicenna J Med Biotechnol 2018 Oct-Dec;10(4):227-232

Department of Biology, Faculty of Biological Sciences, Islamic Azad University, East Tehran Branch, Tehran, Iran.

Background: The Human Epidermal growth factor Receptor-3 (HER-3) is a member of ErbB receptor family and has deficient kinase activity. HER-3 should heterodimerize with other members of ErbB receptor family, especially with HER-2, to transduce downstream signaling pathways. HER-3 co-expresses with other ErbB receptors in different cancers and overexpresses while the oncogenic signaling pathways such as Jak/Stat, MAPK, and PI3K/Akt are activated and promoted. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6252031PMC
December 2018

Effects of . on Matured Mouse Oocytes and Granulosa Cells.

Avicenna J Med Biotechnol 2018 Oct-Dec;10(4):220-226

Department of Biology, Faculty of Sciences, Shiraz University, Shiraz, Iran.

Background: According to previous studies, L. (dill) aqueous extracts decreased the fertility of female rats. Therefore, the present study aimed to examine the effects of this herb on cultured granulosa cells and immature oocytes. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6252029PMC
December 2018
1 Read

Effects of Dietary Polyunsaturated Fatty Acids on DNA Methylation and the Expression of and Genes in Rats.

Avicenna J Med Biotechnol 2018 Oct-Dec;10(4):214-219

Department of Biochemistry, Faculty of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.

Background: Previous studies have suggested a protective role for Polyunsaturated Fatty Acids (PUFA) against cancer, cardiovascular, and other diseases. To provide new insights into the effects of PUFA on gene expression, the effects of dietary PUFA on and gene expression and global DNA methylation were investigated in selected rat tissues.

Methods: Thirty sprague-dawley rats were allotted into 3 dietary groups of ten animals each, received experimental diets containing PUFAs every day by gavages for 12 weeks as follows: control group fed a normal diet and water; n-3 PUFAs group received 300 n-3 PUFAs supplementation; mixed-PUFAs group received 300 of a mixture of n-3, -6, -9 PUFAs supplementations. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6252034PMC
December 2018
2 Reads

Improvement Efficacy of Influenza Nanovaccine in Combination with Hemokinin-1 Molecular Adjuvant.

Avicenna J Med Biotechnol 2018 Oct-Dec;10(4):208-213

Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran.

Background: H9N2 avian influenza viruses have the potential to become the next human pandemic threat and next generation vaccine technologies are needed. Current studies introduce nanoparticles as a proper vaccine delivery vehicle for induction of protective immunity. In this study, the efficacy of chitosan nanoparticle-based H9N2 influenza vaccine with and without hemokinin-1 (HK-1) as a molecular adjuvant to induce protective immunity against the virus was examined. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6252024PMC
December 2018
8 Reads

Inclusion Body Expression and Refolding of Recombinant Bone Morphogenetic Protein-2.

Avicenna J Med Biotechnol 2018 Oct-Dec;10(4):202-207

Department of Medical Biotechnology, Semnan University of Medical Sciences, Semnan, Iran.

Background: Bone Morphogenetic Protein-2 (BMP-2) is a cysteine rich growth factor expressed in homodimeric form and has a pivotal role in osteochondral development and fracture healing. Recent studies have benefited more from recombinant BMP-2 in osteochondral tissue engineering. Cost-effective and easy production at large scale makes () the first choice for recombinant protein expression programs. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6252035PMC
December 2018

An Overview of 3D Bioprinting as a Novel Strategy in the Field of Tissue Engineering.

Avicenna J Med Biotechnol 2018 Oct-Dec;10(4):201

Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6252037PMC
December 2018

Polymorphisms in the Cholinergic Receptors Muscarinic and Genes and Alzheimer's Disease.

Avicenna J Med Biotechnol 2018 Jul-Sep;10(3):196-199

Pengiran Anak Puteri Rashidah Sa'adatul Bolkiah Institute of Health Sciences, Universiti Brunei Darussalam, Jalan Tungku Link, BE 1410, Gadong, Brunei Darussalam.

Background: Disruption of the cholinergic neurotransmitter pathway which is important for cognition, memory and learning abilities has been reported in Alzheimer's Disease (AD) patients. The receptors involved include the Cholinergic Receptors Muscarinic (). gene has been associated with intelligence, personality traits, substance dependence and depression. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6064004PMC
August 2018
6 Reads

Alterations in Turkish Patients with Common Variable Immunodeficiency and IgA Deficiency.

Avicenna J Med Biotechnol 2018 Jul-Sep;10(3):192-195

Department of Pediatric Immunology, Faculty of Medicine, Ege University, Izmir, Turkey.

Background: The Transmembrane Activator and Calcium modulator ligand Interactor (TACI), encoded by gene, is mutated in some patients with Common Variable Immunodeficiency (CVID) and IgA Deficiency (IgAD). The purpose of the study was to investigate for the first time in Turkish patients the prevalence of alterations in CVID, selective and partial IgAD patients.

Methods: Forty two CVID, 36 selective IgAD, 34 partial IgAD and 25 healthy controls were included. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6064010PMC
August 2018
9 Reads

Menstrual Blood-Derived Stromal Stem Cells Augment CD4+ T Cells Proliferation.

Avicenna J Med Biotechnol 2018 Jul-Sep;10(3):183-191

Department of Immunology, Faculty of Public Health, Tehran University of Medical Sciences, Tehran, Iran.

Background: It is more than sixty years that the concept of the fetal allograft and immunological paradox of pregnancy was proposed and in this context, several regulatory networks and mechanisms have been introduced so far. It is now generally recognized that mesenchymal stem cells exert potent immunoregulatory activity. In this study, for the first time, the potential impact of Menstrual blood Stem Cells (MenSCs), as surrogate for endometrial stem cells, on proliferative capacity of CD4+ T cells was tested. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6063997PMC
August 2018
1 Read

Association between rs6759298 and Ankylosing Spondylitis in Iranian Population.

Avicenna J Med Biotechnol 2018 Jul-Sep;10(3):178-182

Rheumatology Research Center, Tehran University of Medical Sciences, Tehran, Iran.

Background: Ankylosing Spondylitis (AS) is a chronic autoinflammatory Spondyloarthropathy (SpA) which is characterized by sacroiliitis, which progresses to the axial skeleton. It seems that non-Human Leukocyte Antigen (HLA) and also HLA-B27 are associated with the susceptibility and pathogenesis of the disease. The recent Ge-nome-Wide Association Studies (GWASs) have reported intergenic rs6759298 to be associated with AS etiology. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6064007PMC
August 2018
2 Reads

and Single Nucleotide Polymorphisms in Iranian Patients with Chronic Heart Failure.

Avicenna J Med Biotechnol 2018 Jul-Sep;10(3):173-177

Molecular Immunology Research Center, Tehran University of Medical Sciences, Tehran, Iran.

Background: Inflammatory cytokines have been known to be associated with Chronic Heart Failure (CHF). Given the importance of cytokines in the context of the failing heart, the prevalence of Interleukin-2 (IL-2) and Interferon-gamma (IFN-γ) polymorphisms was studied in patients with CHF due to ischemic heart disease in a case-control study.

Methods: Fifty-six Iranian patients with CHF were enrolled in this study as the case group and compared with 139 healthy subjects, using polymerase chain reaction with sequence-specific primers method, so as to determine the frequency of alleles, genotypes and haplotypes of (+874 A/T) and (-330 G/T, +166 G/T) SNPs. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6064006PMC
August 2018
17 Reads

Association of the Variations and the Risk of Non-Syndromic Cleft Lip and Palate in a Population of Iranian Infants.

Avicenna J Med Biotechnol 2018 Jul-Sep;10(3):168-172

Genetic Research Center, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran.

Background: Nonsyndromic cleft lip and/or palate (NSCL/P) is the most common orofacial birth defect, often attributed to ethnic and environmental differences. Up to now, linkage analyses and genome-wide association studies have identified several genomic susceptibility regions for NSCL/P. The genes including are strong candidates for NSCL/P, since they are involved in regulating mid-face development and upper lip fusion. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6064000PMC
August 2018
17 Reads

Arylamine N-acetyltransferase 2 Polymorphisms and the Risk of Endometriosis.

Avicenna J Med Biotechnol 2018 Jul-Sep;10(3):163-167

Genetic Research Center, University of Social Welfare and Rehabilitation Sciences, Tehran, Iran.

Background: Human arylamine N-acetyltransferase 2 gene has a key role in xenobiotic metabolism through the conjugation of acetyl group to xenobiotic substances. has been suggested as a susceptibility factor in endometriosis; however, the results of studies have been controversial. In this study, the association of polymorphisms with susceptibility to endometriosis was evaluated in an Iranian population. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6064003PMC
August 2018
10 Reads

Biological Activity of Steroids from the Marine Gastropods Collected from South West Coast of India.

Avicenna J Med Biotechnol 2018 Jul-Sep;10(3):158-162

Department of Chemical Oceanography, Faculty of Marine Sciences, Cochin University of Science and Technology, Cochin-682016, India.

Background: The purpose of this study was to investigate the sterol profiling and predict the pharmacological potential of marine gastropod , collected from mangrove ecosystem in the South west coast of India.

Methods: Sterol fractions were separated from the crude lipids using 15% ethyl acetate. Ethyl acetate fractions were dried under ultrahigh purity N and analyzed using GC-MS. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6064002PMC
August 2018
13 Reads

The Distinct Role of Small Heat Shock Protein 20 on HCV NS3 Expression in HEK-293T Cell Line.

Avicenna J Med Biotechnol 2018 Jul-Sep;10(3):152-157

Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran.

Background: Hepatitis C (HCV) is known as a serious blood-borne disease that infects millions of people globally. NS3 is a conserved non-structural sequence of hepatitis C virus which has a major role in activating specific CTL responses. As known, there is no effective vaccine against HCV infection, thus it is required to design a specific regimen of vaccination. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6063998PMC
August 2018
11 Reads

A Feasibility Study to Evaluate as a Host for Producing Recombinant Human Parathyroid Hormone.

Avicenna J Med Biotechnol 2018 Jul-Sep;10(3):147-151

Department of Molecular Genetics, Research Centre for Biosciences and Biotechnology, Malek-Ashtar University of Technology, Tehran, Iran.

Background: Biosynthetic teriparatide (1-34) (TPD) is a N-terminally truncated version of human parathyroid hormone (hPTH). The recombinant form of this polypeptide has been expressed in and approved as the first anabolic treatment of osteoporosis in the EU and the USA. Feasibility of expression and secretion of a tag- fused form of TPD into was examined due to several advantages of over in production of recombinant proteins with pharmacological activities. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6064005PMC

Soluble Expression of Humanized Anti-CD20 Single Chain Antibody in by Cytoplasmic Chaperones Co-expression.

Avicenna J Med Biotechnol 2018 Jul-Sep;10(3):141-146

Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

Background: CD20 is an important cell surface receptor that is used for target therapy of B cell lymphoma and some related blood diseases due to vital function of CD20. In previous studies, a Rituximab based humanized single chain variable fragment (scFv) antibody showed good reactivity against B cell related cancer cells. But this recombinant protein produced Inclusion Bodies (IBs) in ) cytoplasm. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6063999PMC
August 2018
6 Reads

Gene's Immune Response Induced by Cocktail DNA Vaccine with Gene Against Cutaneous Leishmaniasis in BALB/c Mice.

Avicenna J Med Biotechnol 2018 Jul-Sep;10(3):134-140

Private Veterinary Physician, Gorgan, Iran.

Background: Leishmaniasis is caused by parasitic protozoa of the genus which is an obligate intracellular parasite in the infected host. Individuals who have been recovered from clinical leishmaniasis develop strong immunity against reinfection. DNA vaccines are the new type of vaccines that induce expression of protein eukaryotic cells. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6064009PMC

Apoptosis of Adipose-Derived Stem Cells Induced by Liposomal Soybean Phosphatidylcholine Extract.

Avicenna J Med Biotechnol 2018 Jul-Sep;10(3):126-133

Department of Dermatovenerology, Faculty of Medicine, University of Indonesia, West Java, Indonesia.

Background: Recently, Phosphatidylcholine (PC) has been used as an off-label treatment for lipolysis injection, which is associated with inflammatory reaction due to sodium deoxycholate, an emulsifier, so that inflammation as side effect occurs in those patients. Liposome formulation from lipid was thought to be a better and safer alternative. This study aimed to analyze the mechanism of Liposomal Soybean Phosphatidylcholine (LSPC) extract from Indonesian soybeans (containing 26% PC) to induce Adipose-derived Stem Cells (ASCs) death . Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6064001PMC

Monoclonal Antibody for Reducing Memory and Learning Problems in Schizophrenia.

Avicenna J Med Biotechnol 2018 Jul-Sep;10(3):125

Psychiatric Research Center, Roozbeh Hospital, Tehran University of Medical, Sciences, Tehran, Iran.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6064008PMC
August 2018
2 Reads

Assessment of Gene Expression Following Vitrification of 2-cell and Blastocyst Mouse Embryos.

Avicenna J Med Biotechnol 2018 Apr-Jun;10(2):120-122

Cellular and Molecular Research Center, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran.

Background: Exact mechanisms of fetal harm following vitrification are still unknown. This study was conducted to evaluate the cryopreservation impact on the expression of Epidermal gene in mouse 2-cell and blastocysts.

Methods: To stimulate ovulation in mice, hCG was injected, followed by collecting 2-cells and blastocysts after 44-46 and 88-89 , respectively. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5960058PMC
June 2018
2 Reads

Evaluating the Frequency of , , , and Genes in Aminoglycosides Resistant Isolates Obtained from Hospitalized Patients in Yazd, Iran.

Avicenna J Med Biotechnol 2018 Apr-Jun;10(2):115-119

Department of Public Medicine, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.

Background: is an opportunistic pathogen that could be resistant to many antimicrobial agents. Resistance genes can be carried among gram-negative bacteria by integrons. Enzymatic inactivation is the most important mechanism of resistance to aminoglycosides. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5960057PMC