138,131 results match your criteria Automated Software Engineering[Journal]


Automating Decision Making in the Cryo-EM Pre-processing Pipeline.

Structure 2020 Jul;28(7):727-729

National Resource for Automated Molecular Microscopy, Simons Electron Microscopy Center, New York Structural Biology Center, 89 Convent Ave, New York, NY 10027, USA. Electronic address:

In this issue of Structure, Li et al. (2020) describe machine learning methods that automate decision making in the cryo-EM pre-processing pipeline, eliminating the need for user-subjective decisions. They successfully tested this pipeline for a wide range of datasets, demonstrating the proof of concept and the efficiency of this approach. Read More

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http://dx.doi.org/10.1016/j.str.2020.06.004DOI Listing

A new approach for diagnosing hematological malignancies using monocytosis workflow optimization and abnormal lymphocyte/blast flag of Sysmex XN series of blood count analyzers.

Int J Lab Hematol 2020 Jul 8. Epub 2020 Jul 8.

Department of Medical Biochemistry, Eskisehir Osmangazi University Medical Faculty, Eskisehir, Turkey.

Introduction: Monocytosis Workflow Optimization rule set has been developed by using mono-dysplasia-score to determine reactive monocytosis and prevent unnecessary blood smear of these patients and for detection of chronic myelomonocytic leukemia cases during complete blood count. In our study, we aimed to examine the contribution of Monocytosis Workflow Optimization rule set.

Methods: Adult patients with monocyte count ≥1. Read More

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http://dx.doi.org/10.1111/ijlh.13281DOI Listing

A Multicenter, Cross-Sectional Quality Improvement Project: The Perioperative Implementation of a Hypertension Protocol by Anesthesiologists.

Anesth Analg 2020 Jul 2. Epub 2020 Jul 2.

From the Department of Anaesthesia and Perioperative Medicine.

Background: Hypertension is a common risk factor for cardiovascular morbidity and mortality, with a high prevalence in patients presenting for elective surgery. In limited resource environments, patients have poor access to primary care physicians, limiting the efficacy of lifestyle modification for the management of hypertension. In these circumstances, the perioperative period presents a unique opportunity for diagnosis and initiation and/or modification of pharmacotherapy of hypertension. Read More

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http://dx.doi.org/10.1213/ANE.0000000000004966DOI Listing

Lower Corneal Haze and Aberrations in Descemet Membrane Endothelial Keratoplasty Versus Descemet Stripping Automated Endothelial Keratoplasty in Fellow Eyes for Fuchs Endothelial Corneal Dystrophy.

Cornea 2020 Jul 6. Epub 2020 Jul 6.

Department of Ophthalmology, The University of Texas Southwestern Medical Center, Dallas, TX.

Purpose: To investigate the long-term corneal changes in patients with Fuchs endothelial corneal dystrophy contributing to superior postoperative visual outcomes after Descemet membrane endothelial keratoplasty (DMEK) compared with Descemet stripping automated endothelial keratoplasty (DSAEK).

Methods: Using retrospective analysis, we evaluated 9 patients with Fuchs endothelial corneal dystrophy who underwent DSAEK in 1 eye and DMEK in the fellow eye. Patients were genotyped for the triplet repeat expansion in the TCF4 gene and imaged using optical coherence tomography, Scheimpflug imaging, and in vivo confocal microscopy through focusing. Read More

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http://dx.doi.org/10.1097/ICO.0000000000002416DOI Listing

AutoPH4: An automated method for generating pharmacophore models from protein binding pockets.

J Chem Inf Model 2020 Jul 8. Epub 2020 Jul 8.

Pharmacophore models are widely used in computational drug discovery (e.g., in the virtual screening of drug molecules) to capture essential information about interactions between ligands and a target protein. Read More

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http://dx.doi.org/10.1021/acs.jcim.0c00121DOI Listing

Automated Coupling of Nanodroplet Sample Preparation with Liquid Chromatography-Mass Spectrometry for High-Throughput Single-Cell Proteomics.

Anal Chem 2020 Jul 8. Epub 2020 Jul 8.

Single-cell proteomics can provide critical biological insight into the cellular heterogeneity that is masked by bulk-scale analysis. We have developed a nanoPOTS (nanodroplet processing in one pot for trace samples) platform and demonstrated its broad applicability for single-cell proteomics. However, because of nanoliter-scale sample volumes, the nanoPOTS platform is not compatible with automated LC-MS systems, which significantly limits sample throughput and robustness. Read More

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http://dx.doi.org/10.1021/acs.analchem.0c01551DOI Listing

Effectiveness of the automated drug dispensing system: systematic review and meta-analysis.

Rev Bras Enferm 2020 6;73(5):e20180942. Epub 2020 Jul 6.

Universidade Federal do Estado do Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil.

Objectives: to compare the effectiveness of the decentralized automated drug dispensing system with pockets.

Methods: an effectiveness study based on a systematic review guided by the question: for patients admitted to hospital units, is the use of automated drug dispensing effective for reducing medication errors when compared to manual unit dose dispensing? The evidence was evaluated by the Grading of Recommendations Assessment, Development and Evaluation and the Preferred Reporting Items for Systematic Reviews and Meta-Analyses instrument, used in the report.

Results: the sample was composed of 15 studies and none of them directly compared both technologies; however, the meta-analysis showed that there is no difference in effectiveness between them [OR 1. Read More

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http://dx.doi.org/10.1590/0034-7167-2018-0942DOI Listing

Association between antioxidant vitamins and oxidative stress among patients with a complete hydatidiform mole.

Clinics (Sao Paulo) 2020 6;75:e1724. Epub 2020 Jul 6.

Division of Gynecologic Oncology, Department of Obstetrics and Gynecology and Reproductive Biology, New England Trophoblastic Disease Center, Brigham and Women's Hospital, Dana-Farber Cancer Institute, Boston, MA, USA.

Objectives: This study aimed to evaluate the potential relationship between oxidative stress, dietary intake, and serum levels of antioxidants in patients with a complete hydatidiform mole (CHM) compared with controls.

Methods: This was an observational cross-sectional study conducted in Rio de Janeiro, Brazil. A total of 140 women were enrolled in this study and divided into four groups: 43 patients with CHM, 33 women who had had an abortion, 32 healthy pregnant women, and 32 healthy non-pregnant women. Read More

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http://dx.doi.org/10.6061/clinics/2020/e1724DOI Listing

High-throughput phenotyping of brachiaria grass seeds using free access tool for analyzing X-ray images.

An Acad Bras Cienc 2020 6;92 Suppl 1:e20190209. Epub 2020 Jul 6.

Universidade Federal de Viçosa/UFV, Departamento de Agronomia, Viçosa, MG, Brazil.

New approaches based on image analysis can assist in phenotyping of biological characteristics, serving as support for decision-making in modern agribusiness. The aim of this study was to propose a method of high-throughput phenotyping of free access for processing of 2D X-ray images of brachiaria grass (Brachiaria ruziziensis cv. Ruziziensis) seeds, as well as correlate the parameters linked to the physiological potential of the seeds. Read More

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http://dx.doi.org/10.1590/0001-3765202020190209DOI Listing

Evaluation of Cost, Workflow, and Safety of Implementing a Vial Transfer Device for Ready-to-Mix Drugs at an Academic Medical Center.

J Pharm Pract 2020 Jul 8:897190020938195. Epub 2020 Jul 8.

Department of Pharmacy Services, Brigham and Women's Hospital, Boston, MA, USA.

Objective: To evaluate the cost, workflow, and safety of implementing a vial transfer device system.

Methods: In this retrospective analysis, pharmacy systems and electronic health record reports identified high-volume and high-cost medications prepared by a Vial2Bag (V2B) system from July 2017 to June 2018. The major outcome was the extrapolated yearly cost avoidance (EYCA) from utilization of a V2B system, calculated by subtracting total costs of the V2B system from total cost of ready-to-use products and locally compounded sterile products. Read More

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http://dx.doi.org/10.1177/0897190020938195DOI Listing

Convolutional Neural Network-Based Pelvic Floor Structure Segmentation Using Magnetic Resonance Imaging in Pelvic Organ Prolapse.

Med Phys 2020 Jul 7. Epub 2020 Jul 7.

Biomedical Engineering Department, Peking University, Beijing, 100191, China.

Purpose: Automated segmentation could improve the efficiency of modeling-based pelvic organ prolapse (POP) evaluations. However, segmentation performance is limited by the blurry soft tissue boundaries. In this study, we aimed to present a hybrid solution for uterus, rectum, bladder, and levator ani muscle segmentation by combining a convolutional neural network (CNN) and a level set method. Read More

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http://dx.doi.org/10.1002/mp.14377DOI Listing

CcpNmr AnalysisScreen, a new software programme with dedicated automated analysis tools for fragment-based drug discovery by NMR.

J Biomol NMR 2020 Jul 7. Epub 2020 Jul 7.

Department of Molecular and Cell Biology, Leicester Institute of Structural and Chemical Biology, University of Leicester, Henry Wellcome Building, Lancaster Road, Leicester, LE1 7HN, UK.

Fragment-based drug discovery or FBDD is one of the main methods used by industry and academia for identifying drug-like candidates in early stages of drug discovery. NMR has a significant impact at any stage of the drug discovery process, from primary identification of small molecules to the elucidation of binding modes for guiding optimisations. The essence of NMR as an analytical tool, however, requires the processing and analysis of relatively large amounts of single data items, e. Read More

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http://dx.doi.org/10.1007/s10858-020-00321-1DOI Listing

Histogram analysis for bedside respiratory monitoring in not critically ill preterm neonates: a proposal for a new way to look at the monitoring data.

Eur J Pediatr 2020 Jul 8. Epub 2020 Jul 8.

Department of Neonatology, Lancashire Women and Newborn Centre, East Lancashire Hospital NHS Trust, Casterton Avenue, Burnley, BB10 2PQ, United Kingdom.

Despite robust evidence in favour of maintaining optimal oxygen saturation targets in the preterm infants, the titration of oxygen is largely dependent on manual observations and transcription. Similarly, notwithstanding the gaining popularity of non-invasive modalities like high-flow nasal therapy, the practices of weaning and escalating support are largely individualized and based on point of care observations. These are often erroneous and lack objectivity. Read More

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http://dx.doi.org/10.1007/s00431-020-03732-2DOI Listing

The Minimum Information about a Molecular Interaction Causal Statement (MI2CAST).

Bioinformatics 2020 Jul 8. Epub 2020 Jul 8.

Department of Biology, Norwegian University of Science and Technology (NTNU), Trondheim, Norway.

Motivation: A large variety of molecular interactions occurs between biomolecular components in cells. When a molecular interaction results in a regulatory effect, exerted by one component onto a downstream component, a so-called 'causal interaction' takes place. Causal interactions constitute the building blocks in our understanding of larger regulatory networks in cells. Read More

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http://dx.doi.org/10.1093/bioinformatics/btaa622DOI Listing

Analytical and clinical performance of the fully-automated LIAISONXL calprotectin immunoassay from DiaSorin in IBD patients.

Pract Lab Med 2020 Aug 24;21:e00175. Epub 2020 Jun 24.

Laboratory of Hematology and Clinical Chemistry, OLVG Oost, Amsterdam, the Netherlands.

Objectives: Distinction between inflammatory bowel disease (IBD) and irritable bowel syndrome (IBS) based on clinical symptoms is often difficult. In this study we assessed the performance of the fully-automated calprotectin immunoassay from DiaSorin in IBD diagnosis and follow-up and compared it to the EliA calprotectin 2 immunoassay.

Design: and Methods: The calprotectin immunoassay from DiaSorin run on the LIAISONXL was analytically and clinically validated and compared to the EliA calprotectin 2 immunoassay from Thermo Fisher Scientific run on the ImmunoCAP250. Read More

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http://dx.doi.org/10.1016/j.plabm.2020.e00175DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7327250PMC

Target depth-regularized reconstruction in diffuse optical tomography using ultrasound segmentation as prior information.

Biomed Opt Express 2020 Jun 28;11(6):3331-3345. Epub 2020 May 28.

Electrical and System Engineering Department, Washington University in St. Louis, 1 Brooking Dr, St. Louis, MO 63130, USA.

Ultrasound (US)-guided diffuse optical tomography (DOT) is a promising non-invasive functional imaging technique for diagnosing breast cancer and monitoring breast cancer treatment response. However, because larger lesions are highly absorbing, reconstructions of these lesions using reflection geometry may exhibit light shadowing, which leads to inaccurate quantification of their deeper portions. Here we propose a depth-regularized reconstruction algorithm combined with a semi-automated interactive neural network (CNN) for depth-dependent reconstruction of absorption distribution. Read More

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http://dx.doi.org/10.1364/BOE.388816DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7316021PMC

Machine learning approach to predict medication overuse in migraine patients.

Comput Struct Biotechnol J 2020 12;18:1487-1496. Epub 2020 Jun 12.

BioBIM (InterInstitutional Multidisciplinary Biobank), IRCCS San Raffaele Pisana, Via di Val Cannuta 247, 00166 Rome, Italy.

Machine learning (ML) is largely used to develop automatic predictors in migraine classification but automatic predictors for medication overuse (MO) in migraine are still in their infancy. Thus, to understand the benefits of ML in MO prediction, we explored an automated predictor to estimate MO risk in migraine. To achieve this objective, a study was designed to analyze the performance of a customized ML-based decision support system that combines support vector machines and Random Optimization (RO-MO). Read More

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http://dx.doi.org/10.1016/j.csbj.2020.06.006DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7327028PMC

Electron microscopy-based semi-automated characterization of aggregation in monoclonal antibody products.

Comput Struct Biotechnol J 2020 11;18:1458-1465. Epub 2020 Jun 11.

Kusuma School of Biological Sciences, Indian Institute of Technology - Delhi, Hauz Khas, New Delhi 110016, India.

Aggregation is a critical parameter for protein-based therapeutics, due to its impact on the immunogenicity of the product. The traditional approach towards characterization of such products is to use a collection of orthogonal tools. However, the fact that none of these tools is able to completely classify the distribution and physical characteristics of aggregates, implies that there exists a need for additional analytical methods. Read More

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http://dx.doi.org/10.1016/j.csbj.2020.06.009DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7327430PMC

Constructing knowledge graphs and their biomedical applications.

Comput Struct Biotechnol J 2020 2;18:1414-1428. Epub 2020 Jun 2.

Department of Systems Pharmacology and Translational Therapeutics, Perelman School of Medicine, University of Pennsylvania, Childhood Cancer Data Lab, Alex's Lemonade Stand Foundation, United States.

Knowledge graphs can support many biomedical applications. These graphs represent biomedical concepts and relationships in the form of nodes and edges. In this review, we discuss how these graphs are constructed and applied with a particular focus on how machine learning approaches are changing these processes. Read More

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http://dx.doi.org/10.1016/j.csbj.2020.05.017DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7327409PMC

Next-Generation Manufacturing Protocols Enriching T CAR T Cells Can Overcome Disease-Specific T Cell Defects in Cancer Patients.

Front Immunol 2020 19;11:1217. Epub 2020 Jun 19.

Innovative Immunotherapies Unit, Division of Immunology, Transplantation and Infectious Diseases, IRCCS San Raffaele Scientific Institute, Milan, Italy.

Chimeric antigen receptor (CAR) T cell expansion and persistence emerged as key efficacy determinants in cancer patients. These features are typical of early-memory T cells, which can be enriched with specific manufacturing procedures, providing signal one and signal two in the proper steric conformation and in the presence of homeostatic cytokines. In this project, we exploited our expertise with paramagnetic beads and IL-7/IL-15 to develop an optimized protocol for CAR T cell production based on reagents, including a polymeric nanomatrix, which are compatible with automated manufacturing the CliniMACS Prodigy. Read More

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http://dx.doi.org/10.3389/fimmu.2020.01217DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7317024PMC

A Novel Method of Visual Field Assessment for Patients with Unilateral Severely Limited Central Vision: A Pilot Study.

Clin Ophthalmol 2020 29;14:1799-1804. Epub 2020 Jun 29.

Department of Ophthalmology, Hadassah Medical Center, Kiryat Hadassah, Jerusalem 91120, Israel.

Purpose: Reliable visual field testing requires the tested eye to be fixated on a central target. This poses a major obstacle for eyes with severe central vision loss. This pilot study assesses whether it may be feasible to examine such patients with a modified method. Read More

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http://dx.doi.org/10.2147/OPTH.S258949DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7334015PMC

Cryo-EM structure of arabinosyltransferase EmbB from Mycobacterium smegmatis.

Nat Commun 2020 Jul 7;11(1):3396. Epub 2020 Jul 7.

Department of Physiology and Cellular Biophysics, Columbia University, New York, NY, 10032, USA.

Arabinosyltransferase B (EmbB) belongs to a family of membrane-bound glycosyltransferases that build the lipidated polysaccharides of the mycobacterial cell envelope, and are targets of anti-tuberculosis drug ethambutol. We present the 3.3 Å resolution single-particle cryo-electron microscopy structure of Mycobacterium smegmatis EmbB, providing insights on substrate binding and reaction mechanism. Read More

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http://dx.doi.org/10.1038/s41467-020-17202-8DOI Listing

Characterizing soundscapes across diverse ecosystems using a universal acoustic feature set.

Proc Natl Acad Sci U S A 2020 Jul 7. Epub 2020 Jul 7.

Department of Life Sciences, Imperial College London, Ascot, SL5 7PY, United Kingdom.

Natural habitats are being impacted by human pressures at an alarming rate. Monitoring these ecosystem-level changes often requires labor-intensive surveys that are unable to detect rapid or unanticipated environmental changes. Here we have developed a generalizable, data-driven solution to this challenge using eco-acoustic data. Read More

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http://dx.doi.org/10.1073/pnas.2004702117DOI Listing

Interventions to improve uptake of urate-lowering therapy in patients with gout: a systematic review.

BJGP Open 2020 Jul 7. Epub 2020 Jul 7.

Department of General Practice & Primary Health Care, The University of Auckland, Auckland, New Zealand

Background: Gout treatment is suboptimal despite available therapy, with low levels of initiation and persistence of urate-lowering therapy (ULT) in many patients.

Aim: To identify all interventions that have attempted to improve the uptake of ULT and analyse the clinical outcomes.

Design & Setting: A systematic review of international articles published in English. Read More

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http://dx.doi.org/10.3399/bjgpopen20X101051DOI Listing

Maturation of the auditory system in normal-hearing newborns with a very or extremely premature birth.

Pediatr Neonatol 2020 Jun 12. Epub 2020 Jun 12.

Department of Neonatology, Isala Clinics, Zwolle, the Netherlands.

Background: Literature shows that lower gestational age leads to greater delays in the auditory conduction, which suggests atypical maturation of the brainstem in normal-hearing premature newborns. Our aim is to investigate if there is a difference between the extrauterine and intrauterine maturation of the auditory system in normal-hearing newborns with a very premature (28-31 weeks) or extremely premature (<28 weeks) birth.

Methods: Results of the Automated Auditory Brainstem Response Newborn Hearing Screening Program in Dutch Neonatal Intensive Care Units and diagnostic examinations were centrally registered from 1998 to 2016. Read More

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http://dx.doi.org/10.1016/j.pedneo.2020.05.014DOI Listing

Automated Quantitative Assessment of Coronary Calcification Using Intravascular Ultrasound.

Ultrasound Med Biol 2020 Jul 4. Epub 2020 Jul 4.

Department of Cardiology, Erasmus University Medical Center, Rotterdam, The Netherlands. Electronic address:

Coronary calcification represents a challenge in the treatment of coronary artery disease by stent placement. It negatively affects stent expansion and has been related to future adverse cardiac events. Intravascular ultrasound (IVUS) is known for its high sensitivity in detecting coronary calcification. Read More

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http://dx.doi.org/10.1016/j.ultrasmedbio.2020.04.032DOI Listing

A new automated system for the preparation of sclerosant foam: A study of the physical characteristics produced and the device settings required.

Phlebology 2020 Jul 7:268355520937615. Epub 2020 Jul 7.

Vascular Surgery, Clínica Vascular Barcelona, Barcelona, Spain.

Objectives: To determine the quality of sclerosant foam produced with an automated preparation system with multiple combinations of sclerosing agents, gases, and device settings.

Methods: Single-use mixing capsules filled with air or O/CO and polidocanol (0.25, 1, or 3%) or sodium tetradecyl sulfate (0. Read More

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http://dx.doi.org/10.1177/0268355520937615DOI Listing

Advances on Measuring Deep-Seated Ground Deformations Using Robotized Inclinometer System.

Sensors (Basel) 2020 Jul 5;20(13). Epub 2020 Jul 5.

Italian National Research Council, Research Institute for Hydrogeological Prevention and Protection, Geohazard Monitoring Group (GMG) Strada delle Cacce, 73, 10135 Torino, Italy.

In the field of geo-hazards and geo-engineering, monitoring networks represent a key element for the geological risk assessment and the design and management of large infrastructures construction. In the last decade, we have observed a strong development on remote sensing techniques but just small changes in the subsoil observations. However, this type of measurement is very important to have a three-dimensional representation of the studied area, since the surface measurements often represent a sum of deformations that develop in a complex way in the subsoil. Read More

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http://dx.doi.org/10.3390/s20133769DOI Listing

New Procedure for BIM Characterization of Architectural Models Manufactured Using Fused Deposition Modeling and Plastic Materials in 4.0 Advanced Construction Environments.

Polymers (Basel) 2020 Jul 4;12(7). Epub 2020 Jul 4.

Department of Engineering Graphics Design and Projects, University of Jaen, Campus Las Lagunillas, s/n, Building, A3-210 23071 Jaen, Spain.

This paper presents a new procedure for the building information modeling (BIM) characterization of structural topologies manufactured with plastic materials and fused deposition modeling (FDM) additive technology. The procedure presented here transforms the architectural geometry into an expanded three-dimensional model, capable of directly linking the topology of the plastic structure with the technological, functional and economic requirements for working in advanced construction 4.0 environments. Read More

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http://dx.doi.org/10.3390/polym12071498DOI Listing

Ensemble Deep Learning for Cervix Image Selection toward Improving Reliability in Automated Cervical Precancer Screening.

Diagnostics (Basel) 2020 Jul 3;10(7). Epub 2020 Jul 3.

Communications Engineering Branch, Lister Hill National Center for Biomedical Communications, U.S. National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA.

Automated Visual Examination (AVE) is a deep learning algorithm that aims to improve the effectiveness of cervical precancer screening, particularly in low- and medium-resource regions. It was trained on data from a large longitudinal study conducted by the National Cancer Institute (NCI) and has been shown to accurately identify cervices with early stages of cervical neoplasia for clinical evaluation and treatment. The algorithm processes images of the uterine cervix taken with a digital camera and alerts the user if the woman is a candidate for further evaluation. Read More

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http://dx.doi.org/10.3390/diagnostics10070451DOI Listing

Toward Automated Inventory Modeling in Life Cycle Assessment: The Utility of Semantic Data Modeling to Predict Real-World Chemical Production.

ACS Sustain Chem Eng 2017 Dec;6(2):1961-1976

U.S. Environmental Protection Agency, National Risk Management Research Laboratory, 26 West Martin Luther King Drive, Cincinnati, Ohio 45268, United States.

A set of coupled semantic data models, i.e., ontologies, are presented to advance a methodology toward automated inventory modeling of chemical manufacturing in life cycle assessment. Read More

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http://dx.doi.org/10.1021/acssuschemeng.7b03379DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7336534PMC
December 2017

A Web-Based Tool to Automate Portions of Pretest Genetic Counseling for Inherited Cancer.

J Natl Compr Canc Netw 2020 Jul;18(7):841-847

2Division of Genetic Medicine, Department of Medicine, Vanderbilt University Medical Center, and.

Background: Increasing demand for genetic testing for inherited cancer risk coupled with a shortage of providers trained in genetics highlight the potential for automated tools embedded in the clinic process to meet this demand. We developed and tested a scalable, easy-to-use, 12-minute web-based educational tool that included standard pretest genetic counseling elements related to panel-based testing for multiple genes associated with cancer risk.

Methods: The tool was viewed by new patients at the Vanderbilt Hereditary Cancer Clinic before meeting with a board-certified genetics professional. Read More

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http://dx.doi.org/10.6004/jnccn.2020.7546DOI Listing

AI-PLAX: AI-based placental assessment and examination using photos.

Comput Med Imaging Graph 2020 Jun 1;84:101744. Epub 2020 Jun 1.

The Pennsylvania State University, University Park, PN, USA. Electronic address:

Post-delivery analysis of the placenta is useful for evaluating health risks of both the mother and baby. In the U.S. Read More

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http://dx.doi.org/10.1016/j.compmedimag.2020.101744DOI Listing

Performance evaluation of the spectral autocorrelation function and autoregressive models for automated sleep apnea detection using single-lead ECG signal.

Comput Methods Programs Biomed 2020 Jun 26;195:105626. Epub 2020 Jun 26.

Department of Biomedical Engineering, Tarbiat Modares University, Tehran, Iran. Electronic address:

Background And Objective: This paper addresses the automated recognition of obstructive sleep apnea (OSA) from the analysis of single-lead ECG signals. This is one of the most important problems that is, critical to the realization of monitoring patients with sleep apnea.

Methods: In the present study, a novel solution based on autoregressive (AR) modeling of the single-lead ECG, and spectral autocorrelation function as an ECG feature extraction method is presented. Read More

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http://dx.doi.org/10.1016/j.cmpb.2020.105626DOI Listing

Human pluripotent stem cells: A toolbox to understand and treat retinal degeneration.

Mol Cell Neurosci 2020 Jul 4:103523. Epub 2020 Jul 4.

INSERM U861, I-Stem, AFM, Institute for Stem Cell Therapy and Exploration of Monogenic Diseases, 91100 Corbeil-Essonnes, France; Université Paris-Saclay, Université d'Evry, U861, 91100 Corbeil-Essonnes, France; Centre d'Etude des Cellules Souches, 91100 Corbeil-Essonnes, France. Electronic address:

Age-related Macular Degeneration (AMD) and Retinitis Pigmentosa (RP) are retinal degenerative disorders that dramatically damage the retina. As there is no therapeutic options for the majority of patients, vision is progressively and irremediably lost. Owing to their unlimited renewal and potency to give rise to any cell type of the human adult body, human pluripotent stem cells (hPSCs) have been extensively studied in recent years to develop more physiologically relevant in vitro cellular models. Read More

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http://dx.doi.org/10.1016/j.mcn.2020.103523DOI Listing

Use of Automated Office Blood Pressure Measurement in the Evaluation of Elevated Blood Pressures in Children and Adolescents.

J Pediatr 2020 Jul 4. Epub 2020 Jul 4.

Professor of Pediatrics, University of Washington School of Medicine, Division of Nephrology, Seattle Children's Hospital.

Objectives: To determine the level of agreement between AOBP, auscultated or manual office BP (MOBP) and 24 hour ABPM, and to explore the ability of AOBP and MOBP to correctly identify daytime ambulatory hypertension (HTN) in children.

Study Design: We retrospectively compared BPs obtained by AOBP and MOBP to predict daytime HTN on ABPM. Six BPs were taken by AOBP followed by MOBP. Read More

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http://dx.doi.org/10.1016/j.jpeds.2020.06.059DOI Listing

Electrocardiogram Standards for Children and Young Adults Using Z-scores.

Circ Arrhythm Electrophysiol 2020 Jul 7. Epub 2020 Jul 7.

Rady Children's Hospital San Diego & University of California San Diego, Department of Pediatrics, San Diego, CA.

- Normative ECG values for children are based on relatively few subjects and are not standardized, resulting in interpersonal variability of interpretation. Recent advances in digital technology allow a more quantitative, reproducible assessment of ECG variables. Our objective was to create the foundation of normative ECG standards in the young utilizing Z-scores. Read More

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http://dx.doi.org/10.1161/CIRCEP.119.008253DOI Listing

A Comprehensive Targeted Metabolomic Assay for Urine Analysis.

Anal Chem 2020 Jul 7. Epub 2020 Jul 7.

Among all the human biological fluids used for disease biomarker discovery or clinical chemistry, urine stands out. It can be col-lected easily and non-invasively, it is readily available in large volumes, it is typically free from protein contamination and it is chemically complex - reflecting a wide range of physiological states and functions. However, the comprehensive metabolomic analysis of urine has been somewhat less studied compared to blood. Read More

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http://dx.doi.org/10.1021/acs.analchem.0c01682DOI Listing

Implementation of a multiple-fraction concatenation strategy in an online two-dimensional high-/low-pH reversed-phase/reversed-phase liquid chromatography platform for qualitative and quantitative shotgun proteomic analyses.

J Mass Spectrom 2020 Jun 12:e4591. Epub 2020 Jun 12.

Department of Chemistry, The University of Hong Kong, Hong Kong, China.

Multidimensional liquid chromatography is the mainstay separation technique used for shotgun proteomic analyses. The application of a multiple-fraction concatenation (MFC) strategy can result in a more disperse and consistent peptide elution profile across different fractions, when compared with a conventional strategy. Herein, we present the first automated online RP-RP platform implementing an MFC strategy to facilitate robust, unattended, routine proteomic analyses. Read More

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http://dx.doi.org/10.1002/jms.4591DOI Listing

Military Protein Intake Related to Strength and Fat Mass Independent of Energy Intake.

Mil Med 2020 Jul 7. Epub 2020 Jul 7.

Sports Medicine Research Institute, University of Kentucky, 720 Sports Center Dr. Lexington, KY 40506.

Introduction: Kinetic military units operate in austere training environments and deprivation not commonly experienced by competitive athletes. Nutritional strategies to protect against decrements in performance and potential injury risk may differ for these two groups. A cross sectional analysis was conducted to determine energy and macronutrient characteristics associated with performance metrics. Read More

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http://dx.doi.org/10.1093/milmed/usaa151DOI Listing

An in vivo microfluidic study of bacterial transit in C. elegans nematodes.

Lab Chip 2020 Jul 7. Epub 2020 Jul 7.

Laboratory of Microsystems, Ecole Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland.

Caenorhabditis elegans (C. elegans) constitutes an important model organism for use in nutrition and aging studies. We report a novel method for studying the dynamics of Escherichia coli (E. Read More

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http://dx.doi.org/10.1039/d0lc00064gDOI Listing

What's Driving Me? Exploration and Validation of a Hierarchical Personality Model for Trust in Automated Driving.

Hum Factors 2020 Jul 6:18720820922653. Epub 2020 Jul 6.

98917 9189 Ulm University, Germany.

Objective: This paper presents a comprehensive investigation of personality traits related to trust in automated vehicles. A hierarchical personality model based on Mowen's (2000) 3M model is explored in a first and replicated in a second study.

Background: Trust in automation is established in a complex psychological process involving user-, system- and situation-related variables. Read More

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http://dx.doi.org/10.1177/0018720820922653DOI Listing

Novel semi-automated algorithm for high-throughput quantification of adipocyte size in breast adipose tissue, with applications for breast cancer microenvironment.

Adipocyte 2020 Dec;9(1):313-325

BU-BMC Cancer Center, Boston University School of Medicine , Boston, MA, USA.

The size distribution of adipocytes in fat tissue provides important information about metabolic status and overall health of patients. Histological measurements of biopsied adipose tissue can reveal cardiovascular and/or cancer risks, to complement typical prognosis parameters such as body mass index, hypertension or diabetes. Yet, current methods for adipocyte quantification are problematic and insufficient. Read More

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http://dx.doi.org/10.1080/21623945.2020.1787582DOI Listing
December 2020

High-precision stable isotope analysis of <5 μg CaCO samples by continuous-flow mass spectrometry.

Rapid Commun Mass Spectrom 2020 Jul 7:e8878. Epub 2020 Jul 7.

Department of Climate Geochemistry, Max Planck Institute for Chemistry, Hahn-Meitner-Weg 1, Mainz, 55128, Germany.

Rationale: Oxygen (δ O) and carbon (δ C) isotope analysis of foraminifera and other CaCO samples has been a key technique for paleoceanographical and paleoclimatological research for more than 60 years now. There is ongoing demand for the analysis of ever smaller CaCO samples, driven, for example, by the desire to analyse single specimen planktic foraminifera, or small samples of tooth enamel.

Methods: We present a continuous-flow mass spectrometric technique, that uses cryo-focussing of sample CO to analyse CaCO samples in a weight range between 10 and 3 μg. Read More

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http://dx.doi.org/10.1002/rcm.8878DOI Listing

High-Yielding Radiosynthesis of [Ga]Ga-PSMA-11 Using a Low-Cost Microfluidic Device.

Mol Imaging Biol 2020 Jul 6. Epub 2020 Jul 6.

Vanderbilt Center for Molecular Probes, Vanderbilt University Medical Center, Nashville, TN, 37232, USA.

Purpose: Current PET radiotracer production models result in facility and operational costs that scale prohibitively with the number of tracers synthesized, particularly those made as a single dose-on-demand. Short of a paradigm shift in the technology and economics of radiotracer production, the impact of PET on precision medicine will be limited. Inexpensive, microfluidic radiochemistry platforms have the potential to significantly reduce costs associated with dose-on-demand production and expand the breadth of PET tracers accessible for molecular imaging. Read More

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http://dx.doi.org/10.1007/s11307-020-01515-7DOI Listing

A meta-analysis of the predictability of LENA™ automated measures for child language development.

Dev Rev 2020 Sep 11;57. Epub 2020 Jun 11.

Department of Otolaryngology-Head & Neck Surgery, The Ohio State University, 915 Olentangy River Road # 4000, Columbus, OH.

Early language environment plays a critical role in child language development. The Language ENvironment Analysis (LENA™) system allows researchers and clinicians to collect daylong recordings and obtain automated measures to characterize a child's language environment. This meta-analysis evaluates the predictability of LENA's automated measures for language skills in young children. Read More

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http://dx.doi.org/10.1016/j.dr.2020.100921DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7337141PMC
September 2020

Automated spheroid generation, drug application and efficacy screening using a deep learning classification: a feasibility study.

Sci Rep 2020 Jul 6;10(1):11071. Epub 2020 Jul 6.

Institute of Molecular Medicine and Cell Research, Albert Ludwigs University of Freiburg, Stefan Meier Strasse 17, 79104, Freiburg, Germany.

The last two decades saw the establishment of three-dimensional (3D) cell cultures as an acknowledged tool to investigate cell behaviour in a tissue-like environment. Cells growing in spheroids differentiate and develop different characteristics in comparison to their two-dimensionally grown counterparts and are hence seen to exhibit a more in vivo-like phenotype. However, generating, treating and analysing spheroids in high quantities remains labour intensive and therefore limits its applicability in drugs and compound research. Read More

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http://dx.doi.org/10.1038/s41598-020-67960-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7338379PMC

Development of simple and efficient Lab-on-a-Disc platforms for automated chemical cell lysis.

Sci Rep 2020 Jul 6;10(1):11039. Epub 2020 Jul 6.

Molecular Bank Iranian Biological Resource Center (IBRC), ACECR Tehran, Tehran, Iran.

Cell lysis is the most important first step for molecular biology and diagnostic testing. Recently, microfluidic systems have attracted considerable attention due to advantages associated with automation, integration and miniaturization, especially in resource-limited settings. In this work, novel centrifugal microfluidic platforms with new configurations for chemical cell lysis are presented. Read More

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http://dx.doi.org/10.1038/s41598-020-67995-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7338454PMC

Structural basis for the stabilization of amyloidogenic immunoglobulin light chains by hydantoins.

Bioorg Med Chem Lett 2020 Aug 16;30(16):127356. Epub 2020 Jun 16.

Department of Chemistry, The Scripps Research Institute, La Jolla, CA 92037, USA; The Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, CA 92037, USA. Electronic address:

Misfolding and aggregation of immunoglobulin light chains (LCs) leads to the degeneration of post-mitotic tissue in the disease immunoglobulin LC amyloidosis (AL). We previously reported the discovery of small molecule kinetic stabilizers of the native dimeric structure of full-length LCs, which slow or stop the LC aggregation cascade at the outset. A predominant structural category of kinetic stabilizers emerging from the high-throughput screen are coumarins substituted at the 7-position, which bind at the interface between the two variable domains of the light chain dimer. Read More

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http://dx.doi.org/10.1016/j.bmcl.2020.127356DOI Listing

Comparison of smartphone-based retinal imaging systems for diabetic retinopathy detection using deep learning.

BMC Bioinformatics 2020 Jul 6;21(Suppl 4):259. Epub 2020 Jul 6.

Dept. of Computer Science, University of Central Arkansas, 201 Donaghey Ave, Conway, AR, 72035, USA.

Background: Diabetic retinopathy (DR), the most common cause of vision loss, is caused by damage to the small blood vessels in the retina. If untreated, it may result in varying degrees of vision loss and even blindness. Since DR is a silent disease that may cause no symptoms or only mild vision problems, annual eye exams are crucial for early detection to improve chances of effective treatment where fundus cameras are used to capture retinal image. Read More

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http://dx.doi.org/10.1186/s12859-020-03587-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7336606PMC