111,459 results match your criteria Attachment & Human Development[Journal]


TLR2/4 signaling pathway mediates sperm-induced inflammation in bovine endometrial epithelial cells in vitro.

PLoS One 2019 17;14(4):e0214516. Epub 2019 Apr 17.

Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan.

We have recently shown that sperm attachment to bovine endometrial epithelial cells (BEECs) triggers uterine local innate immunity with induction of a pro-inflammatory response in vitro, however details of the mechanism remain unknown. Here, we investigated the involvement of Toll-like receptor 2/4 (TLR2/4) pathway in mediating sperm-BEECs inflammatory process. Immunohistochemistry of the uterine tissue revealed that TLR2 and TLR4 proteins were present in the luminal and glandular epithelia of bovine endometrium. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0214516PLOS

Monoclonal antibodies point to Achilles' heel in picornavirus capsid.

Authors:
Mihnea Bostina

PLoS Biol 2019 Apr 17;17(4):e3000232. Epub 2019 Apr 17.

Department of Microbiology and Immunology and Otago Micro and Nano Imaging, University of Otago, Dunedin, New Zealand.

Picornaviruses are small, icosahedral, nonenveloped, positive-sense, single-stranded RNA viruses that form one of the largest and most important viral families. Numerous Picornaviridae members pose serious health or agricultural threats, causing diseases such as poliomyelitis, hepatitis A, or foot-and-mouth disease. The antigenic characterization of picornavirus capsids plays an important role in understanding the mechanism of viral neutralization and the conformational changes associated with genome release, and it can point to regions which can be targeted by small-molecule compounds to be developed as antiviral inhibitors. Read More

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http://dx.doi.org/10.1371/journal.pbio.3000232DOI Listing

Translation and Brazilian adaptation of the Relationship Scales Questionnaire (RSQ).

Trends Psychiatry Psychother 2019 Jan-Mar;41(1):69-77. Epub 2019 Apr 11.

Programa de Pós-Graduação em Gerontologia Biomédica, Escola de Medicina, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, RS, Brazil.

Objectives: To describe the process of translating and adapting the Relationship Scales Questionnaire (RSQ) from English into Brazilian Portuguese and to present the results of its test-retest reliability using the version developed for interview application.

Methodology: The process was based on the guidelines of the International Society for Pharmaeconomics and Outcomes Research (ISPOR), which propose 10 steps for the translation and cross-cultural adaptation of self-administered instruments. The original authors of the RSQ have agreed to the translation. Read More

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http://dx.doi.org/10.1590/2237-6089-2018-0032DOI Listing

The role of long-term psychodynamic psychotherapy in improving attachment patterns, defense styles, and alexithymia in patients with depressive/anxiety disorders.

Trends Psychiatry Psychother 2019 Jan-Mar;41(1):43-50. Epub 2019 Apr 11.

Social Determinants of Health Research Center, Babol University of Medical Sciences, Babol, Iran.

Introduction: Long-term psychodynamic psychotherapy (LTPP) emphasizes the centrality of intrapsychic and unconscious conflicts and their relation to development. Although there is evidence supporting the efficacy of LTPP in mental disorders, little research has been published on the efficacy of LTPP for depressive and anxiety disorders.

Objective: To examine whether patients with anxiety and depressive disorders demonstrate improvement in their attachment styles, defense styles, psychiatric symptoms, anxiety/depressive symptoms, and alexithymia with LTPP. Read More

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http://www.scielo.br/scielo.php?script=sci_arttext&pid=S
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http://dx.doi.org/10.1590/2237-6089-2017-0144DOI Listing
April 2019
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Frequency of Porphyromonas gingivalis fimA in smokers and nonsmokers after periodontal therapy.

J Appl Oral Sci 2019 11;27:e20180205. Epub 2019 Apr 11.

Universidade Federal Fluminense, Departamento de Odontologia, Área de Periodontia, Nova Friburgo, Rio de Janeiro, Brasil.

Porphyromonas gingivalis is one of the most important Gram-negative anaerobe bacteria involved in the pathogenesis of periodontitis. P. gingivalis has an arsenal of specialized virulence factors that contribute to its pathogenicity. Read More

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http://www.scielo.br/scielo.php?script=sci_arttext&pid=S
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http://dx.doi.org/10.1590/1678-7757-2018-0205DOI Listing
April 2019
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Membrane-permeant, environment-sensitive dyes generate biosensors within living cells.

J Am Chem Soc 2019 Apr 16. Epub 2019 Apr 16.

Dyes with environment-sensitive fluorescence have proven useful to study the spatio-temporal dynamics of protein activity in living cells. When attached to proteins, their fluorescence can reflect protein conformational changes, posttranslational modifications or protein interactions. However, the utility of such dye-protein conjugates has been limited because it is difficult to load them into cells. Read More

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http://dx.doi.org/10.1021/jacs.8b09841DOI Listing

Evaluation of Vaccination Coverages in the Health Care Workers of a University Hospital in Southern Italy.

Ann Ig 2019 Mar-Apr;31(2 Supple 1):13-24

Postgraduate Medical School in Hygiene and Preventive Medicine, University of Messina, Italy.

Introduction: An adequate immunization of the health care workers is essential for infection prevention and control, to avoid consequences not only for them, but for all patients that the health care workers could infect causing serious damage and / or death. Unfortunately, to date, despite the presence of international and national guidelines (Law Decree 119/2017), the vaccination coverage was low, also because of the "vaccine hesitancy" spread among the same health care workers. In light of the above, the aim of our study was to investigate the vaccination coverage of healthcare workers of all the operational units present in our hospital and to evaluate differences between sex, age, professional profile and area of work. Read More

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http://dx.doi.org/10.7416/ai.2019.2273DOI Listing

Intraoperative quantification of meningioma cell proliferation potential using rapid flow cytometry reveals intratumoral heterogeneity.

Cancer Med 2019 Apr 16. Epub 2019 Apr 16.

Department of Neurosurgery, Saitama Medical Center, Saitama Medical University, Saitama, Japan.

Background: Standard sampling methods to evaluate the proliferative ability of meningioma have not been established.

Methods: This prospective study was conducted to evaluate the effectiveness of intraoperative rapid flow cytometry (iFC) using raw samples for the quantitative assessment of proliferative ability in meningioma cells and to investigate intratumoral heterogeneity. Proliferation index (PI) was defined as the ratio of aneuploid cells with an abnormal number of chromosomes to the total cells. Read More

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http://dx.doi.org/10.1002/cam4.2178DOI Listing

Periodontitis as the risk factor of chronic kidney disease: Mediation analysis.

J Clin Periodontol 2019 Apr 16. Epub 2019 Apr 16.

Section for Clinical Epidemiology and Biostatistics, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand.

Aim: To determine sequences and magnitude of causality among periodontitis, diabetes and chronic kidney disease (CKD) by mediation analysis.

Methods: Ten-year-data were retrieved from the Electric Generation Authority of Thailand (EGAT) study. A cohort of 2,635 subjects was identified with no CKD at baseline. Read More

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http://dx.doi.org/10.1111/jcpe.13114DOI Listing

12R-lipoxygenase activity is reduced in photodamaged facial stratum corneum. A novel activity assay indicates a key function in corneocyte maturation.

Int J Cosmet Sci 2019 Apr 16. Epub 2019 Apr 16.

UCL School of Pharmacy, London, UK.

Background: During the late stage of keratinocyte differentiation corneocytes gain a strong protein-lipid structure: the corneocyte envelopes (CE), composed of the inner corneocyte protein envelope (CPE) and the outer corneocyte lipid envelope (CLE). The hydrophobicity of CEs depends on the covalent attachment of linoleoyl-acyl-ceramides by transglutaminases (TG). These ceramides are processed by a range of other enzymes, including 12R-lipoxygenase (12R-LOX), before the covalent attachment of the free ω-hydroxyceramides to the CPE surface to form the CLE. Read More

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http://dx.doi.org/10.1111/ics.12532DOI Listing

Separation anxiety and gender variance in a community sample of children.

Eur Child Adolesc Psychiatry 2019 Apr 16. Epub 2019 Apr 16.

Department of Psychology, University of Toronto Mississauga, Mississauga, ON, L5L 1C6, Canada.

In clinical child and retrospective adult samples, childhood gender variance (GV; i.e., cross-gender behaviour) has been associated with separation anxiety (SA; i. Read More

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http://link.springer.com/10.1007/s00787-019-01319-3
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http://dx.doi.org/10.1007/s00787-019-01319-3DOI Listing
April 2019
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Carvacrol antimicrobial wash treatments reduce Campylobacter jejuni and aerobic bacteria on broiler chicken skin.

Poult Sci 2019 Apr 16. Epub 2019 Apr 16.

Poultry Production and Product Safety Research Unit, USDA-ARS, AR 72701, USA.

Campylobacter jejuni, a major cause of human gastroenteritis worldwide, is often associated with the consumption of contaminated poultry products. With increasing consumer preference to natural and minimally processed foods, interventions utilizing natural antimicrobials for controlling C. jejuni on poultry products are gaining popularity. Read More

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http://dx.doi.org/10.3382/ps/pez198DOI Listing

Tuning the stability of alkoxyisopropyl protection groups.

Beilstein J Org Chem 2019 21;15:746-751. Epub 2019 Mar 21.

Department of Chemistry, University of Helsinki, P.O.Box 55 (A.I.Virtasen aukio 1), FIN-00014 Helsinki, Finland.

Five different 2-alkoxypropan-2-yl groups are introduced as acid-labile protecting groups for the 5'- and 3'-hydroxy groups of a 2'-deoxynucleoside. All studied protecting groups were readily introduced with good to excellent yields using the appropriate enol ether as a reagent and 0.5 to 1 mol % toluenesulfonic acid as a catalyst. Read More

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http://dx.doi.org/10.3762/bjoc.15.70DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6444389PMC

Superparamagnetic iron oxide nanoparticles combined with NGF and quercetin promote neuronal branching morphogenesis of PC12 cells.

Int J Nanomedicine 2019 27;14:2157-2169. Epub 2019 Mar 27.

Department of Biotechnology, Faculty of Advanced Sciences and Technologies, University of Isfahan, Isfahan, Iran.

Background: The investigation of agents promoting recovery of nerve regeneration following neurodegenerative diseases has been the most important issue in neuroscience. Nerve growth factor (NGF) and quercetin as potential flavonoids could possibly have therapeutic applications in the field of degenerative diseases such as Parkinson and Alzheimer.

Materials And Methods: The MTT assay was done at 24, 48, and 72 hours to examine the cytotoxicity of superparamagnetic iron oxide nanoparticles (SPIONs) and quercetin. Read More

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http://dx.doi.org/10.2147/IJN.S191878DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6445231PMC

Structure-based Design of JOC-x, a Conjugatable Tumor Tight Junction Opener to Enhance Cancer Therapy.

Sci Rep 2019 Apr 16;9(1):6169. Epub 2019 Apr 16.

PAI Life Sciences, Inc., Seattle, WA, USA.

Disorganized intercellular junctions are critical for maintaining the integrity of solid epithelial tumors and prevent the infiltration of oncological therapies into the bulk of the malignancy. We have developed small, recombinant proteins which bind a critical junction protein, desmoglein 2, triggering the transient and specific opening of tumor tight junctions allowing for infiltration of the tumor with immune cells, oncolytic viruses, drugs, and other therapeutics. Our new molecule, JOC-x, is a promising candidate for a new class of tumor-targeting agents that accumulate both around and within tumors and remodel the tumor microenvironment. Read More

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http://www.nature.com/articles/s41598-019-42229-3
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http://dx.doi.org/10.1038/s41598-019-42229-3DOI Listing
April 2019
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An apically located hybrid guanylate cyclase-ATPase is critical for the initiation of Ca signalling and motility in .

J Biol Chem 2019 Apr 16. Epub 2019 Apr 16.

Division of Infection and Immunity, The Walter and Eliza Hall Institute of Medical Research, Australia.

Protozoan parasites of the phylum Apicomplexa actively move through tissue in order to initiate and perpetuate infection. The regulation of parasite motility relies on cyclic nucleotide-dependent kinases, but how these kinases are activated remains unknown. Here, using an array of biochemical and cell biology approaches, we show that the apicomplexan parasite expresses a large guanylate cyclase protein (TgGC), which contains several upstream ATPase transporter-like domains. Read More

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http://dx.doi.org/10.1074/jbc.RA118.005491DOI Listing

Variable non-linear removal of viruses during transport through a saturated soil column.

J Contam Hydrol 2019 Apr 10. Epub 2019 Apr 10.

Department of Soil, Water and Environmental Science, The University of Arizona, Tucson, AZ, United States.

Reduction of viral surrogates (bacteriophage MS2 and murine norovirus-1 [MNV-1]) and viruses naturally present in wastewater (enteroviruses, adenoviruses, Aichi viruses, reovirus, pepper mild mottle virus) was studied in a long-term experiment simulating soil-aquifer treatment of a non-disinfected secondary treated wastewater effluent blend using a 4.4 m deep saturated soil column (95% sand, 4% silt, 1% clay) with a hydraulic residence time of 15.4 days under predominantly anoxic redox conditions. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01697722183041
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http://dx.doi.org/10.1016/j.jconhyd.2019.04.002DOI Listing
April 2019
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Unraveling Interaction Mechanisms between Molybdenite and Dodecane Oil Droplet Using Atomic Force Microscopy.

Langmuir 2019 Apr 16. Epub 2019 Apr 16.

Molybdenite (MoS2) is a mineral that has drawn great interest because of its potential application in various fields. To facilitate the flotation of molybdenite, the mineral pulp is commonly treated with non-polar oil additives to promote hydrophobicity and to form an oil bridge between ultrafine molybdenite particles for agglomeration. In this study, dodecane was chosen as a model oil to investigate the flotation mechanisms of molybdenite by non-polar oil. Read More

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http://dx.doi.org/10.1021/acs.langmuir.9b00203DOI Listing

The Polyphenol-Rich Extract from an Endemic Medicinal Plant from Reunion Island, Inhibits the Early Stages of Dengue and Zika Virus Infection.

Int J Mol Sci 2019 Apr 15;20(8). Epub 2019 Apr 15.

Université de la Réunion, INSERM U1187, CNRS UMR 9192, IRD UMR 249, Unité Mixte Processus Infectieux en Milieu Insulaire Tropical, Plateforme Technologique CYROI, Sainte Clotilde, 97490 La Réunion, France.

The recent emergence and re-emergence of viral infections transmitted by vectors, such as the Zika virus (ZIKV) and Dengue virus (DENV), is a cause for international concern. These highly pathogenic arboviruses represent a serious health burden in tropical and subtropical areas of the world. Despite the high morbidity and mortality associated with these viral infections, antiviral therapies are missing. Read More

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http://dx.doi.org/10.3390/ijms20081860DOI Listing

Effect of Jute Fiber Modification on Mechanical Properties of Jute Fiber Composite.

Materials (Basel) 2019 Apr 15;12(8). Epub 2019 Apr 15.

College of Textiles, Donghua University, 2999 North Renmin Road, Shanghai 201620, China.

Recently, the demand for reinforced plastics from natural, sustainable, biodegradable, and environmentally friendly fibers has been rising worldwide. However, the main shortcoming of natural fibers reinforced plastics is the poor compatibility between reinforcing fibers and the matrix. Hence, it is necessary to form a strong attachment of the fibers to the matrix to obtain the optimum performance. Read More

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http://dx.doi.org/10.3390/ma12081226DOI Listing

Host-parasite interaction as a toxicity test endpoint using asymmetrical exposures.

Aquat Toxicol 2019 Apr 8;211:173-180. Epub 2019 Apr 8.

University of South Bohemia in České Budějovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zátiší 728/II, 389 25 Vodňany, Czech Republic.

Interspecific relationships frequently determine the effect a pollutant can have on an organism, and this is especially true in closely interacting species such as hosts and parasites. The high spatial and temporal variability of contaminant concentrations combined with the movement of aquatic biota can further influence the consequences that are associated with contamination. We used a full factorial design for the exposed and unexposed partners of the relationship between the parasitic larvae (glochidia) of the European freshwater mussel (Anodonta anatina) and its host fish (Squalius cephalus) to identify the sources of variation in the sublethal endpoints of species interaction (the intensity of parasite attachment, the spatial position of glochidia on the host body, and encapsulation success). Read More

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http://dx.doi.org/10.1016/j.aquatox.2019.04.006DOI Listing
April 2019
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Treatment of Gingival Recessions Associated with Noncarious Cervical Lesions Using Natural Inlays Before Coronally Advanced Flap Application: A Case Series.

Int J Periodontics Restorative Dent 2019 Apr 16. Epub 2019 Apr 16.

Treatment of gingival recessions associated with noncarious cervical lesions (NCCL) can be a challenging clinical situation for clinicians. Both periodontal and restorative treatment can be necessary for this kind of defect. By using both digitally precise computer-aided design/computer-assisted manufacture (CAD/CAM) procedures and natural inlay (NI) material for restoration of defects before the coronally advanced flap (CAF) surgery, attachment of soft tissues on the root surface can be achieved successfully. Read More

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http://dx.doi.org/10.11607/prd.3543DOI Listing

Narrative Coherence in Adolescence: Relations With Attachment, Mentalization, and Psychopathology.

J Pers Assess 2019 Apr 16:1-10. Epub 2019 Apr 16.

a Department of Psychology , Northeastern University.

Narrative coherence refers to the overall completeness of the narrative that helps the individual to draw meaning from past events. Research has predominantly focused on developmental trajectories of narrative coherence among typically developing individuals and less research sheds light on narrative coherence in adolescents facing serious psychological difficulties. This study is the first to apply Baerger and McAdams's well-validated coding scheme of narrative coherence to adolescents and to rate narrative coherence based on the content derived from the Child Attachment Interview in the context of attachment security, mentalization, and internalizing and externalizing pathology in 70 inpatient adolescents. Read More

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https://www.tandfonline.com/doi/full/10.1080/00223891.2019.1
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http://dx.doi.org/10.1080/00223891.2019.1574805DOI Listing
April 2019
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In a father's mind: paternal reflective functioning, sensitive parenting, and protection against socioeconomic risk.

Attach Hum Dev 2019 Apr 16:1-22. Epub 2019 Apr 16.

e Department of Psychological Science , University of California , Irvine , CA , USA.

This study utilized attachment theory as a framework for understanding how fathers' reflective functioning (RF) and social emotional (SE) and autonomy (AU) supportive behaviors relate to children's emotion regulation (ER) beyond effects of mothers' RF. Moreover, the study explored how fathers' RF may be a protective factor against risks associated with low income. Fathers (n = 77) and their toddlers participated. Read More

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http://dx.doi.org/10.1080/14616734.2019.1582596DOI Listing

Sex and Laterality Differences in Medullary Humerus Morphology.

Anat Rec (Hoboken) 2019 Apr 15. Epub 2019 Apr 15.

Rehabilitation Research and Development Service, VA Medical Center, 500 Foothill Drive (151), Salt Lake City, Utah.

Percutaneous osseointegrated (OI) prosthetic limb attachment holds promise for transhumeral amputees. Understanding humeral medullary morphology is necessary for informed design of upper extremity OI systems, and is beneficial to the field of megaprosthetic reconstruction of the distal humerus where diaphyseal fixation is desired. The purpose of this study was to quantify the sex and laterality differences in humerus morphology, specifically over the diaphysis. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/ar.24138
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http://dx.doi.org/10.1002/ar.24138DOI Listing
April 2019
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Molecular interplay of autophagy and endocytosis in human health and diseases.

Biol Rev Camb Philos Soc 2019 Apr 15. Epub 2019 Apr 15.

Department of Life Science, National Institute of Technology Rourkela, Sundergarh, Odisha 769008, India.

Autophagy, an evolutionarily conserved process for maintaining the physio-metabolic equilibrium of cells, shares many common effector proteins with endocytosis. For example, tethering proteins involved in fusion like Ras-like GTPases (Rabs), soluble N-ethylmaleimide sensitive factor attachment protein receptors (SNAREs), lysosomal-associated membrane protein (LAMP), and endosomal sorting complex required for transport (ESCRT) have a dual role in endocytosis and autophagy, and the trafficking routes of these processes converge at lysosomes. These common effectors indicate an association between budding and fusion of membrane-bound vesicles that may have a substantial role in autophagic lysosome reformation, by sensing cellular stress levels. Read More

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http://dx.doi.org/10.1111/brv.12515DOI Listing

Membrane biofouling retardation by zwitterionic peptide and its impact on the bacterial adhesion.

Environ Sci Pollut Res Int 2019 Apr 15. Epub 2019 Apr 15.

Shandong Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Shandong University, Qingdao, 266237, China.

Nanofiltration polyamide membranes naturally tend towards biofouling, due to their surface physicochemistries. Nisin, a type of short cationic amphiphilic peptide with antimicrobial properties, has been recognized as a safe antimicrobial for food biopreservation and biomedical applications. This study investigates the impact of nisin on the initial bacterial attachment to membranes, its anti-biofouling properties, and characterizes a non-monotonic correlation between nisin concentration and biofilm inhibition. Read More

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http://link.springer.com/10.1007/s11356-019-04898-5
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http://dx.doi.org/10.1007/s11356-019-04898-5DOI Listing
April 2019
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Decellularized Adipose Tissue: Biochemical Composition, in vivo Analysis and Potential Clinical Applications.

Adv Exp Med Biol 2019 Apr 16. Epub 2019 Apr 16.

Center for Stem Cell Research & Regenerative Medicine, Tulane University School of Medicine, New Orleans, LA, USA.

Decellularized tissues are gaining popularity as scaffolds for tissue engineering; they allow cell attachment, proliferation, differentiation, and are non-immunogenic. Adipose tissue is an abundant resource that can be decellularized and converted in to a bio-scaffold. Several methods have been developed for adipose tissue decellularization, typically starting with freeze thaw cycles, followed by washes with hypotonic/hypertonic sodium chloride solution, isopropanol, detergent (SDS, SDC and Triton X-100) and trypsin digestion. Read More

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http://dx.doi.org/10.1007/5584_2019_371DOI Listing

Lesson Learnt from Recall of Valsartan and Other Angiotensin II Receptor Blocker Drugs Containing NDMA and NDEA Impurities.

AAPS PharmSciTech 2019 Apr 15;20(5):166. Epub 2019 Apr 15.

Irma Lerma Rangel College of Pharmacy, Texas A&M Health Science Center, Texas A&M University, 310 Reynolds Medical Building, Suite 159, College Station, Texas, 77843-1114, USA.

The presence of N-nitrosodimethylamine (NDMA) and N-nitrosodiethylamine (NDEA) impurities in angiotensin II receptor blocker (ARB) drugs containing tetrazole ring has triggered worldwide product recalls. The purpose of this article is to identify the potential gap area in current pharmaceutical industry practice that might have led to the NMDA and NDEA impurities escaping the drug manufacturer's and FDA's attention. The impact of process change was not adequately assessed by the manufacturer of contaminated APIs (active pharmaceutical ingredients), and potential for generation of mutagenic or other toxic impurities was not considered. Read More

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http://dx.doi.org/10.1208/s12249-019-1376-1DOI Listing

New insights into black truffle biology: discovery of the potential connecting structure between a Tuber aestivum ascocarp and its host root.

Mycorrhiza 2019 Apr 15. Epub 2019 Apr 15.

Institut national de la recherche agronomique (INRA), Unité Mixte de Recherche 1136 INRA-Université de Lorraine, Interactions Arbres/Microorganismes, Centre INRA-Grand Est-Nancy, 54280, Champenoux, France.

According to isotopic labeling experiments, most of the carbon used by truffle (Tuber sp.) fruiting bodies to develop underground is provided by host trees, suggesting that trees and truffles are physically connected. However, such physical link between trees and truffle fruiting bodies has never been observed. Read More

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http://link.springer.com/10.1007/s00572-019-00892-4
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http://dx.doi.org/10.1007/s00572-019-00892-4DOI Listing
April 2019
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Effect of Multi-walled Carbon Nanotubes on the Toxicity of Triphenyltin to the Marine Copepod Tigriopus japonicus.

Bull Environ Contam Toxicol 2019 Apr 15. Epub 2019 Apr 15.

School of Food and Environment, Dalian University of Technology, Panjin, 124221, China.

Marine organisms are often exposed to a mixture of various pollutants in marine environment (i.e., nanoparticles, organic pollutants). Read More

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http://dx.doi.org/10.1007/s00128-019-02608-yDOI Listing

Mechanistic insights into the SNARE complex disassembly.

Sci Adv 2019 Apr 10;5(4):eaau8164. Epub 2019 Apr 10.

State Key Laboratory of Membrane Biology, Beijing Advanced Innovation Center for Structural Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China.

NSF (-ethylmaleimide-sensitive factor) and α-SNAP (α-soluble NSF attachment protein) bind to the SNARE (soluble NSF attachment protein receptor) complex, the minimum machinery to mediate membrane fusion, to form a 20S complex, which disassembles the SNARE complex for reuse. We report the cryo-EM structures of the α-SNAP-SNARE subcomplex and the NSF-D1D2 domain in the 20S complex at 3.9- and 3. Read More

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http://dx.doi.org/10.1126/sciadv.aau8164DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6457932PMC

S-nitrosylation of the Peroxiredoxin-2 promotes S-nitrosoglutathione-mediated lung cancer cells apoptosis via AMPK-SIRT1 pathway.

Cell Death Dis 2019 Apr 15;10(5):329. Epub 2019 Apr 15.

Key Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, 5 Yushan Road, Qingdao, 266003, China.

Protein S-nitrosylation, the redox-based posttranslational modification of a cysteine thiol by the attachment of a nitric oxide (NO) group, is responsible for a variety of signaling effects. Dysregulation of S-nitrosylation may be directly linked to cancer apoptotic resistance and cancer therapy outcomes, emphasizing the importance of S-nitrosylation in cancer. Peroxiredoxin-2 (Prdx2), an antioxidant enzyme, plays an important role in the protection of cancer cells from oxidative radical damage caused by hydrogen dioxide (HO), which is a potential target for cancer therapy. Read More

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http://www.nature.com/articles/s41419-019-1561-x
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http://dx.doi.org/10.1038/s41419-019-1561-xDOI Listing
April 2019
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Occurrence and characterisation of biofilms in drinking water systems of broiler houses.

BMC Microbiol 2019 Apr 15;19(1):77. Epub 2019 Apr 15.

Flanders Research Institute for Agriculture, Fisheries and Food (ILVO), Technology and Food Science Unit, Brusselsesteenweg 370, 9090, Melle, Belgium.

Background: Water quality in the drinking water system (DWS) plays an important role in the general health and performance of broiler chickens. Conditions in the DWS of broilers are ideal for microbial biofilm formation. Since pathogens might reside within these biofilms, they serve as potential source of waterborne transmission of pathogens to livestock and humans. Read More

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http://dx.doi.org/10.1186/s12866-019-1451-5DOI Listing

Trauma and anxious attachment influence the relationship between suggestibility and dissociation: a moderated-moderation analysis.

Cogn Neuropsychiatry 2019 Apr 16:1-17. Epub 2019 Apr 16.

a Department of Psychology , Goldsmiths, University of London , London , UK.

Introduction: Hypnotic suggestibility is elevated in the dissociative disorders but the relationship between dissociative tendencies and suggestibility in the general population seems to be constrained by additional factors. The diathesis-stress (DS) model stipulates that suggestibility interacts with trauma exposure to augment the propensity for dissociative states whereas the dual pathway to suggestibility (DPS) model proposes two developmental routes involving either dissociation preceded by trauma, or a healthy cognitive profile characterised by superior imagination.

Methods: This study sought to discriminate between these partially competing accounts and further considered the moderating role of anxious attachment. Read More

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http://dx.doi.org/10.1080/13546805.2019.1606705DOI Listing

Effect of the Immobilization Strategy on the Efficiency and Recyclability of the Versatile Lipase from .

Molecules 2019 Apr 3;24(7). Epub 2019 Apr 3.

Centro de Investigaciones Biológicas (CSIC), C/Ramiro de Maeztu, 9, 28040 Madrid, Spain.

The recombinant lipase from OPEr has demonstrated to have catalytic properties superior to those of many commercial enzymes. Enzymatic crudes with OPEr were immobilized onto magnetite nanoparticles by hydrophobicity (SiMAG-Octyl) and by two procedures that involve covalent attachment of the protein (mCLEAs and AMNP-GA), giving three nanobiocatalysts with different specific activity in hydrolysis of -nitrophenyl butyrate (NPB) and good storage stability at 4 °C over a period of 4 months. Free OPEr and the different nanobiocatalysts were compared for the synthesis of butyl esters of volatile fatty acids C4 to C7 in reactions containing the same lipase activity. Read More

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http://dx.doi.org/10.3390/molecules24071313DOI Listing

Mimicking the Annulus Fibrosus Using Electrospun Polyester Blended Scaffolds.

Nanomaterials (Basel) 2019 Apr 3;9(4). Epub 2019 Apr 3.

School of Materials, Faculty of Science and Engineering, The University of Manchester, Oxford Road, Manchester M13 9PL, UK.

Treatments to alleviate chronic lower back pain, caused by intervertebral disc herniation as a consequence of degenerate annulus fibrosus (AF) tissue, fail to provide long-term relief and do not restore tissue structure or function. This study aims to mimic the architecture and mechanical environment of AF tissue using electrospun fiber scaffolds made from synthetic biopolymers-poly(ε-caprolactone) (PCL) and poly(L-lactic) acid (PLLA). Pure polymer and their blends (PCL%:PLLA%; 80:20, 50:50, and 20:80) are studied and material properties-fiber diameter, alignment, % crystallinity, tensile strength, and water contact angle-characterized. Read More

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http://dx.doi.org/10.3390/nano9040537DOI Listing

Intracellular Delivery of Biologically-Active Fungal Metabolite Gliotoxin Using Magnetic Nanoparticles.

Materials (Basel) 2019 Apr 2;12(7). Epub 2019 Apr 2.

Instituto de Carboquímica (ICB-CSIC), 50018 Zaragoza, Spain.

Gliotoxin (GT), a secondary metabolite produced by Aspergillus molds, has been proposed as a potential anti-tumor agent. Here we have developed a nanoparticle approach to enhance delivery of GT in tumor cells and establish a basis for its potential use as therapeutical drug. GT bound to magnetic nanoparticles (MNPs) retained a high anti-tumor activity, correlating with efficient intracellular delivery, which was increased in the presence of glucose. Read More

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http://dx.doi.org/10.3390/ma12071092DOI Listing

Anti-Herpetic, Anti-Dengue and Antineoplastic Activities of Simple and Heterocycle-Fused Derivatives of Terpenyl-1,4-Naphthoquinone and 1,4-Anthraquinone.

Molecules 2019 Apr 2;24(7). Epub 2019 Apr 2.

Departamento de Ciencias Farmacéuticas, Área de Química Farmacéutica, Facultad de Farmacia, CIETUS, IBSAL. Campus Miguel de Unamuno, University of Salamanca, 37007-Salamanca, Spain.

Quinones are secondary metabolites of higher plants associated with many biological activities, including antiviral effects and cytotoxicity. In this study, the anti-herpetic and anti-dengue evaluation of 27 terpenyl-1,4-naphthoquinone (NQ), 1,4-anthraquinone (AQ) and heterocycle-fused quinone (HetQ) derivatives was done in vitro against Human Herpesvirus (HHV) type 1 and 2, and Dengue virus serotype 2 (DENV-2). The cytotoxicity on HeLa and Jurkat tumor cell lines was also tested. Read More

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http://dx.doi.org/10.3390/molecules24071279DOI Listing

Structure-Guided Immobilization of an Evolved Unspecific Peroxygenase.

Int J Mol Sci 2019 Apr 2;20(7). Epub 2019 Apr 2.

Department of Biocatalysis, Institute of Catalysis, CSIC, Cantoblanco, 28049 Madrid, Spain.

Unspecific peroxygenases (UPOs) are highly promiscuous biocatalyst with self-sufficient mono(per)oxygenase activity. A laboratory-evolved UPO secreted by yeast was covalently immobilized in activated carriers through one-point attachment. In order to maintain the desired orientation without compromising the enzyme's activity, the S221C mutation was introduced at the surface of the enzyme, enabling a single disulfide bridge to be established between the support and the protein. Read More

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https://www.mdpi.com/1422-0067/20/7/1627
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http://dx.doi.org/10.3390/ijms20071627DOI Listing
April 2019
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Long-Term (15 to 20 Years) Outcomes of Papillae Preservation Flap Surgery in Esthetic Areas.

Authors:
Renzo Guarnieri

Int J Periodontics Restorative Dent 2019 May/Jun;39(3):349-359

Several techniques have been proposed to maintain full papillary form and preserve soft tissue during periodontal surgical access. However, very little evidence of long-term results is available. The aim of the present paper is to report the 15- to 20-year follow-up outcomes of a case series study of papillae preservation flap surgery in esthetic areas. Read More

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http://dx.doi.org/10.11607/prd.4017DOI Listing
April 2019
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Heavy-atom labeling of RNA by PLOR for de novo crystallographic phasing.

PLoS One 2019 15;14(4):e0215555. Epub 2019 Apr 15.

Protein-Nucleic Acid Interaction Section, Structural Biophysics Laboratory, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, Maryland, United States of America.

Due to the paucity of known RNA structures, experimental phasing is crucial for obtaining three-dimensional structures of RNAs by X-ray crystallography. Covalent attachment of heavy atoms to RNAs is one of the most useful strategies to facilitate phase determination. However, this approach is limited by the inefficiency or inability to synthesize large RNAs (>60 nucleotides) site-specifically labeled with heavy atoms using traditional methods. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0215555PLOS
April 2019
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Depressive symptom contagion in the transition to parenthood: Interparental processes and the role of partner-related attachment.

J Abnorm Psychol 2019 Apr 15. Epub 2019 Apr 15.

Department of Psychology.

How depressive symptoms unfold within a couple during the perinatal events of pregnancy, childbirth, and early parenthood is poorly understood. In this prospective study, we aim to investigate the reciprocal relation between maternal and paternal depressive symptomatology, specifically how symptoms in 1 partner relate to subsequent symptom level changes in the other partner throughout the perinatal period. Further, we aim to identify parents who are particularly vulnerable to the development of disruptive processes of negative mood states. Read More

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http://dx.doi.org/10.1037/abn0000429DOI Listing

Notable effect of water on excess electron attachment to aqueous DNA deoxyribonucleosides.

Phys Chem Chem Phys 2019 Apr 15. Epub 2019 Apr 15.

State Key Laboratory of Molecular Reaction Dynamics, Dalian Institute of Chemical Physics, Chinese Academy of Science, Zhongshan Road 457, Dalian 116023, China.

Computations using the combined quantum mechanical/molecular mechanical (QM/MM) method were performed to investigate excess electron attachment to and detachment from aqueous deoxyribonucleosides (dRNs). The QM/MM vertical electron affinities (VEAs) of four dRNs are higher than the values of the corresponding nucleobases by ∼0.20 eV. Read More

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http://xlink.rsc.org/?DOI=C9CP00536F
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http://dx.doi.org/10.1039/c9cp00536fDOI Listing
April 2019
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Micro-Nano Bioactive Glass Particles Incorporated Porous Scaffold for Promoting Osteogenesis and Angiogenesis .

Front Chem 2019 29;7:186. Epub 2019 Mar 29.

Department of Biomedical Engineering, School of Materials Science and Engineering, South China University of Technology, Guangzhou, China.

Constructing the interconnected porous biomaterials scaffolds with osteogenesis and angiogenesis capacity is extremely important for efficient bone tissue engineering. Herein, we fabricated a bioactive micro-nano composite scaffolds with excellent osteogenesis and angiogenesis capacity, based on poly (lactic-co-glycolic acid) (PLGA) incorporated with micro-nano bioactive glass (MNBG). The results showed that the addition of MNBG enlarged the pore size, increased the compressive modulus (4 times improvement), enhanced the physiological stability and apatite-forming ability of porous PLGA scaffolds. Read More

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https://www.frontiersin.org/article/10.3389/fchem.2019.00186
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http://dx.doi.org/10.3389/fchem.2019.00186DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6449679PMC
March 2019
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Dissociative Electron Attachment From Vibrationally Excited Molecules in Nanosecond Repetitively Pulsed CO Discharges and Afterglows.

Front Chem 2019 29;7:163. Epub 2019 Mar 29.

P.Las.M.I. Lab, CNR-Nanotec, Bari, Italy.

Non-equilibrium vibrational distributions and electron energy distributions of CO in nanosecond repetitively pulsed (NRP) discharges and afterglows have been determined from a coupled solution of the time dependent Boltzmann equation for the electron energy distribution function (eedf) of free electrons and the master equations for the vibrational distribution function (vdf) of CO and the electronic excited states of CO and O and C atoms. Emphasis is given to the role of dissociative electron attachment (DEA) from vibrationally excited states in affecting the eedf and vdf under extreme conditions, i.e. Read More

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http://dx.doi.org/10.3389/fchem.2019.00163DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6450140PMC

Effect of gutkha chewing on periodontal health and oral hygiene of peoples in Delhi NCR region of North India: A cross-sectional multicentered study.

J Family Med Prim Care 2019 Feb;8(2):564-567

Department of Public Health Dentistry, ITS Centre for Dental Studies and Research, Muradnagar, Ghaziabad, Uttar Pradesh, India.

Background: In India, gutkha is popular among all socioeconomic groups, since it is available easily and is of less cost. Various investigations have demonstrated the relationship of gutkha biting with periodontal status and oral hygiene.

Aim: The aim of this study is to assess the effect of gutkha chewing on periodontal health and oral hygiene of patients attending the dental department in India. Read More

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http://www.jfmpc.com/text.asp?2019/8/2/564/253052
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http://dx.doi.org/10.4103/jfmpc.jfmpc_439_18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6436307PMC
February 2019
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Vascular leakage caused by loss of Akt1 is associated with impaired mural cell coverage.

FEBS Open Bio 2019 Apr 20;9(4):801-813. Epub 2019 Mar 20.

Biomedical Research Institute Gene and Cell Therapy Center for Vessel Associated Disease Department of Pharmacology Pusan National University School of Medicine Yangsan Korea.

Angiogenesis plays a critical role in embryo development, tissue repair, tumor growth and wound healing. In the present study, we investigated the role of the serine/threonine kinase Akt in angiogenesis. Silencing of Akt1 in human umbilical vein endothelial cells significantly inhibited vascular endothelial growth factor (VEGF)-induced capillary-like tube formation. Read More

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http://dx.doi.org/10.1002/2211-5463.12621DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6443864PMC

Effective anti-biofouling enabled by surface electric disturbance from water wave-driven nanogenerator.

Nano Energy 2019 Mar 21;57:558-565. Epub 2018 Dec 21.

Department of Materials Science and Engineering, University of Wisconsin-Madison, Madison, Wisconsin 53706, United States.

Biofouling has been a long-last problem in a variety of marine systems which causes energy waste and device damage. In this study, we present a self-activated anti-biofouling system enabled by electrical double layer disturbance, which could effectively suppress the initial formation of conditioning layers and subsequent microbe attachment. The small and low-frequency alternating electrical fields were produced by a triboelectric nanogenerator under water wave impacts. Read More

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http://dx.doi.org/10.1016/j.nanoen.2018.12.069DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6459608PMC

An elongated dorsally curved xiphoid process.

Anat Cell Biol 2019 Mar 29;52(1):102-104. Epub 2019 Mar 29.

Seattle Science Foundation, Seattle, WA, USA.

The xiphoid process of the sternum lies in the epigastric region and functions to serve as an attachment point for vital muscles that aid in respiration. With the xiphoid process extending as the most inferior portion of the sternum, variable morphology is widely observed. During a routine dissection of a 44-year-old Caucasian male cadaver, we discovered a hook-shaped, elongated xiphoid process that protruded dorsally. Read More

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https://synapse.koreamed.org/DOIx.php?id=10.5115/acb.2019.52
Publisher Site
http://dx.doi.org/10.5115/acb.2019.52.1.102DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6449596PMC
March 2019
1 Read