2,827 results match your criteria Atla-alternatives To Laboratory Animals[Journal]


Refined experimental design may increase the value of murine models for estimation of bluetongue virus virulence.

Lab Anim 2020 Jun 26:23677220930056. Epub 2020 Jun 26.

Department of Production Animal Clinical Sciences, Norwegian University of Life Sciences, Norway.

Bluetongue is a serious non-contagious vector-borne viral disease in ruminants, causing poor animal welfare and economic consequences globally. Concern has been raised about the development of novel bluetongue virus (BTV) strains and their possibly altered virulence through the process of viral reassortment. Virulence is traditionally estimated in lethal dose 50 (LD) studies in murine models, but agreement with both and virulence in ruminants is questionable, and a refined experimental design is needed. Read More

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http://dx.doi.org/10.1177/0023677220930056DOI Listing

Many thanks to our animal caretakers and technicians.

Lab Anim 2020 Jun;54(3):295

FELASA Honorary Secretary, FELASA, Eye, UK.

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http://dx.doi.org/10.1177/0023677220924348DOI Listing

Technical experience and postoperative complications with repeat transperitoneal approach to the lumbar spine in female Lewis rats.

Lab Anim 2020 Jun 15:23677220930043. Epub 2020 Jun 15.

Department of Orthopaedic Surgery, Beaumont Health, USA.

Rat models of lumbar intervertebral disc (IVD) degeneration are widely employed to characterize biologic-based therapeutics, but their anatomy and small size preclude consistent delivery of injectable therapeutics to the lumbar spine via the traditional posterolateral approach. Here, we describe our experience with a repeat ventral transperitoneal approach in female Lewis rats, enabling induction of IVD degeneration and later intervention via an injectable therapeutic. In the initial surgery, the ventral aspect of the L5/L6 IVD was accessed, and an annular defect was created using a #11 scalpel blade. Read More

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http://dx.doi.org/10.1177/0023677220930043DOI Listing

Efficient production of immunodeficient non-obese diabetic/Shi-scid mice via the superovulation technique using inhibin antiserum and gonadotropin.

Lab Anim 2020 Jun 6:23677220928091. Epub 2020 Jun 6.

Center for Animal Resources and Development (CARD), Kumamoto University, Japan.

Severe immunodeficient mice are an essential tool for the examination of the efficacy and safety of new therapeutic technologies as a humanized model. Previously, non-obese diabetic (NOD)/Shi-scid (NOG) mice were established as immunodeficient mice by combining interleukin-2 receptor-γ chain-knockout mice and NOD/Shi-scid mice. The NOG mice are used frequently in the research of therapeutic monoclonal antibodies and regenerative medicine for human diseases. Read More

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http://dx.doi.org/10.1177/0023677220928091DOI Listing

Determination of basal bone mineral density in the femur bones of male and female Wistar rats.

Lab Anim 2020 May 28:23677220922566. Epub 2020 May 28.

Departamento de Nanotecnología, Centro de Física Aplicada y Tecnología Avanzada, Universidad Nacional Autónoma de México, Campus Juriquilla, Mexico.

Changes in bone mineral content of calcium (Ca), phosphorous (P), magnesium and potassium for male and female Wistar rats during their development from 3 weeks old to adulthood (27 weeks old) were measured. Bone mineral content was related to areal bone mineral density (BMD) which was measured in vivo at the femoral neck using a calibrated X-ray transmission system to obtain basal curves as a function of the age of the specimen. Diagnostic curves were built to determine low BMD (osteopaenia) and osteoporosis in female rats fed a Ca-depleted diet (50%) based on the obtained data and the criteria established by the World Health Organization. Read More

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http://dx.doi.org/10.1177/0023677220922566DOI Listing

Application of the 3R principles: Vertebrae as an additional source of murine bone-marrow cells.

Lab Anim 2020 May 16:23677220922573. Epub 2020 May 16.

Blood Transfusion Centre of Slovenia, Slovenia.

Experimental murine models are an essential tool in the field of bone marrow (BM) transplantation research. Therefore, numerous mice are required to obtain a sufficient number of BM cells, which is in contrast with the Reduction principle of the 3R principles. The selection of the cell source and the isolation protocol are therefore critical in obtaining a sufficient yield of cells for experiments. Read More

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http://dx.doi.org/10.1177/0023677220922573DOI Listing

Morphometric analysis of knockout pig kidney and heart.

Lab Anim 2020 Apr 21:23677220915218. Epub 2020 Apr 21.

Animal Biotechnology Division, National Institute of Animal Science, Republic of Korea.

We report a morphometric evaluation of knockout (GTKO) pig heart and kidney ( = 9) at the end of one, three and seven months. Organs parameters gradually increased with the age ( < 0.05) and body weight ( < 0. Read More

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http://dx.doi.org/10.1177/0023677220915218DOI Listing
April 2020
1.120 Impact Factor

Collection on score sheets, severity assessment and humane end points: Invitation to submit.

Lab Anim 2020 Apr;54(2):149

Platform for Morphology and Department of Fundamental Neurosciences, Faculty of Biology and Medicine, University of Lausanne, Switzerland.

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http://dx.doi.org/10.1177/0023677220906399DOI Listing

El XV Congreso de la SECAL (Sociedad Española para las Ciencias del Animal de Laboratorio) tuvo lugar en Sevilla del 6 al 8 de noviembre.

Lab Anim 2020 Apr;54(2):196-197

Animal Facility and Transgenic Unit. CITIUS III, University of Seville, Seville, Spain.

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http://dx.doi.org/10.1177/0023677220906766DOI Listing

3Rs - Reduce Reuse Recycle.

Lab Anim 2020 Apr;54(2):194

FELASA Honorary Secretary, FELASA, Eye, UK.

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http://dx.doi.org/10.1177/0023677220909272DOI Listing

Report on LASA's annual meeting, November 2019.

Authors:
Angela Kerton

Lab Anim 2020 Jun 11;54(3):301-302. Epub 2020 Apr 11.

Chair of the Scientific Programme, LASA, UK.

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http://dx.doi.org/10.1177/0023677220909774DOI Listing

Severity assessment using three common behavioral or locomotor tests after laparotomy in rats: a pilot study.

Lab Anim 2020 Mar 30:23677220911680. Epub 2020 Mar 30.

Institute for Laboratory Animal Science and Experimental Surgery, RWTH Aachen University, Faculty of Medicine, Germany.

The aim of this pilot study was to evaluate whether behavioral or locomotor tests (Open Field (OF), rotarod (RR), and CatWalk (CW)) can help assess the severity of laparotomy in rats. The new EU Directive (2010/63/EU) mandates severity assessment in experiments involving animals. However, validated and objective methods are needed to relate trial-specific monitoring results to the degree of distress caused to individual animals. Read More

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http://dx.doi.org/10.1177/0023677220911680DOI Listing

Use of high frequency jet ventilation as a refinement for imaging macaques with respiratory disease.

Lab Anim 2020 Mar 26:23677220913328. Epub 2020 Mar 26.

Public Health England, National Infection Service, Porton Down, Salisbury, SP4 0JG, UK.

Imaging is used in human medicine to diagnose disease and monitor treatment efficacy. Computed tomography (CT) positron emission tomography (PET) and magnetic resonance (MR) are applied to animal models of infectious diseases to increase data quality, enhance their relevance to the clinical situation, and to address ethical issues through reduction of numbers and refinement of study designs. The time required for collection of MR and PET-CT scans means that normal breathing produces motion artefacts that can render images unacceptable. Read More

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http://dx.doi.org/10.1177/0023677220913328DOI Listing

Refining the unilateral ureteral obstruction mouse model: No sham, no shame.

Lab Anim 2020 Mar 17:23677220909401. Epub 2020 Mar 17.

UCB Pharma, UK.

Fibrosis, as a common final pathway in many renal diseases, contributes significantly to the decline of organ function and to progression to end-stage renal disease. To establish therapeutic interventions that target fibrosis, animal models are essential. The most widely used model of renal fibrosis is the unilateral ureteral obstruction (UUO) model. Read More

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http://dx.doi.org/10.1177/0023677220909401DOI Listing

Experimental replications in animal trials.

Lab Anim 2020 Mar 5:23677220907617. Epub 2020 Mar 5.

Center for Medical Statistics, Informatics and Intelligent Systems, Section for Clinical Biometrics, Medical University Vienna, Austria.

The recent discussion on the reproducibility of scientific results is particularly relevant for preclinical research with animal models. Within certain areas of preclinical research, there exists the tradition of repeating an experiment at least twice to demonstrate replicability. If the results of the first two experiments do not agree, then the experiment might be repeated a third time. Read More

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http://dx.doi.org/10.1177/0023677220907617DOI Listing

A novel mechanical ventilator providing flow-controlled expiration for small animals.

Lab Anim 2020 Feb 19:23677220906857. Epub 2020 Feb 19.

Department of Anesthesiology and Critical Care, University Medical Center Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany.

For investigating the effects of mechanical ventilation on the respiratory system, experiments in small mammal models are used. However, conventional ventilators for small animals are usually limited to a specific ventilation mode, and in particular to passive expiration. Here, we present a computer-controlled research ventilator for small animals which provides conventional mechanical ventilation as well as new type ventilation profiles. Read More

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http://dx.doi.org/10.1177/0023677220906857DOI Listing
February 2020

Sciatic nerve injury model in rabbits: What to expect.

Lab Anim 2020 Feb 18:23677219898481. Epub 2020 Feb 18.

Department of Plastic Surgery, Vanderbilt University Medical Center, USA.

Rabbits are commonly used for sciatic nerve injuries larger than 1.5 cm. This report provides insight into risks and benefits associated with using rabbit models in sciatic nerve injury models and proposes interventions that researchers can use to prevent experimental complications. Read More

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http://dx.doi.org/10.1177/0023677219898481DOI Listing
February 2020

Using multidimensional scaling in model choice for congenital oesophageal atresia: similarity analysis of human autopsy organ weights with those from a comparative assessment of Aachen Minipig and Pietrain piglets.

Lab Anim 2020 Feb 16:23677220902184. Epub 2020 Feb 16.

Department of Paediatric Surgery, Universitätsmedizin Mainz, Germany.

Swine models had been popular in paediatric oesophageal surgery in the past. Although being largely replaced by rodent models, swine experienced a revival with the establishment of minipig models. However, none of them has ever been investigated for similarity to humans. Read More

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http://dx.doi.org/10.1177/0023677220902184DOI Listing
February 2020

A novel welfare and scientific approach to conducting dog metabolism studies allowing dogs to be pair housed.

Lab Anim 2020 Feb 16:23677220905330. Epub 2020 Feb 16.

Novo Nordisk A/S, Måløv, Denmark.

Metabolism cages are designed to conduct absorption, distribution, metabolism and excretion (ADME) studies, enabling an 'excretion balance' scientific objective to be met. Historically, the design of dog metabolism cages has involved single housing. This type of housing has limitations for normal social behaviours and has been largely unchanged for 25-30 years. Read More

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http://dx.doi.org/10.1177/0023677220905330DOI Listing
February 2020

Impulse for animal welfare outside the experiment.

Lab Anim 2020 Apr 12;54(2):150-158. Epub 2020 Feb 12.

Department Animal Welfare and 3Rs, University of Zurich, Switzerland.

Animal welfare is a growing societal concern and the well-being of animals used for experimental purposes is under particular scrutiny. The vast majority of laboratory animals are mice living in small cages that do not offer very much variety. Moreover, the experimental procedure often takes very little time compared to the time these animals have been bred to the desired age or are being held available for animal experimentation. Read More

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http://dx.doi.org/10.1177/0023677219891754DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7160751PMC

Phytoestrogen-containing diets offer benefits for mouse embryology but lead to fewer offspring being produced.

Lab Anim 2020 Feb 12:23677219898486. Epub 2020 Feb 12.

Institute of Zoology, Technische Universität Dresden, Germany.

One of the most commonly used protein sources in rodent diets is soy, which is naturally rich in phytoestrogens. Although phytoestrogens have shown potential health benefits in humans, they may also have the ability to disrupt reproduction. Consequently, there has been a tendency to try to exclude them from rodent diets. Read More

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http://dx.doi.org/10.1177/0023677219898486DOI Listing
February 2020

Efficiency of timed pregnancies in C57BL/6 and BALB/c mice by mating one male with up to four females.

Lab Anim 2020 Feb 11:23677219897687. Epub 2020 Feb 11.

Institute for Laboratory Animal Science, Hannover Medical School, Germany.

For a wide range of biomedical approaches, an accurate estimate of the age of embryos or pups is important. Overnight mating is the method that is mostly used to establish timed pregnancies. The oestrus cycle in mice repeats every four to five days. Read More

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http://dx.doi.org/10.1177/0023677219897687DOI Listing
February 2020

Life cycle of a FELASA working group.

Lab Anim 2020 Feb;54(1):113

FELASA Honorary Secretary, FELASA, Eye, UK.

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http://dx.doi.org/10.1177/0023677219897534DOI Listing
February 2020

Severity Assessment in animal based research.

Lab Anim 2020 Feb;54(1):16

Institute for Laboratory Animal Science and Experimental Surgery, RWTH Aachen University, Germany.

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http://dx.doi.org/10.1177/0023677219898105DOI Listing
February 2020

Nesting material enrichment reduces severity of overgrooming-related self-injury in individually housed rats.

Lab Anim 2020 Jan 10:23677219894356. Epub 2020 Jan 10.

Center for Studies in Behavioral Neurobiology, Department of Psychology, Concordia University, Montreal, Canada.

Individual or singly-housing laboratory rats is common in many animal facilities, but has an adverse impact on the welfare of this social species. It has previously been shown that a small proportion of individually housed mice (∼5%) engage in pathological overgrooming behaviour, but this has not been assessed in rats. We performed an observational study to determine the prevalence of overgrooming-related self-injury and whether providing nesting material enrichment throughout an animal's life would affect the prevalence or severity of overgrooming-related self-injury. Read More

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http://dx.doi.org/10.1177/0023677219894356DOI Listing
January 2020

FELASA's horizon for 2020.

Lab Anim 2019 Dec;53(6):643

FELASA Honorary Secretary, FELASA, UK.

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http://dx.doi.org/10.1177/0023677219885735DOI Listing
December 2019

News from LASA.

Authors:
Angela Kerton

Lab Anim 2019 Dec;53(6):648-650

PO Box 140, Ware, Hertfordshire SG9 0ZN, UK.

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http://dx.doi.org/10.1177/0023677219886432DOI Listing
December 2019

GV-SOLAS Seminar Report.

Authors:
Matthias Schmidt

Lab Anim 2019 Dec;53(6):645-646

Tierschutzbeauftragter, Ruhr-Universität Bochum, Bochum, Germany.

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http://dx.doi.org/10.1177/0023677219886430DOI Listing
December 2019

The European Federation of the Pharmaceutical Industry and Associations' Research and Animal Welfare Group: Assessing and benchmarking 'Culture of Care' in the context of using animals for scientific purpose.

Lab Anim 2019 Nov 19:23677219887998. Epub 2019 Nov 19.

Science Policy and Regulatory Affairs, EFPIA, Belgium.

The European Federation of Pharmaceutical Industries and Associations' Research and Animal Welfare group members reflected on the concept of a Culture of Care in relation to animal care and use and on differences in its understanding and application across European pharmaceutical companies. The term 'Culture of Care' is used across different regions and organizations but rarely with any defined indicators to support working practice. The European Federation of Pharmaceutical Industries and Associations' Research and Animal Welfare group has developed a framework to help organizations identify gaps or potential areas for improvement in support of a positive Culture of Care. Read More

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http://dx.doi.org/10.1177/0023677219887998DOI Listing
November 2019
1.120 Impact Factor

Where are we heading? Challenges in evidence-based severity assessment.

Lab Anim 2020 Feb 13;54(1):50-62. Epub 2019 Nov 13.

Institute for Laboratory Animal Science, Hannover Medical School, Germany.

Evidence-based severity assessment in laboratory animals is, apart from the ethical responsibility, imperative to generate reproducible, standardized and valid data. However, the path towards a valid study design determining the degree of pain, distress and suffering experienced by the animal is lined with pitfalls and obstacles as we will elucidate in this review. Furthermore, we will ponder on the genesis of a holistic concept relying on multifactorial composite scales. Read More

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http://dx.doi.org/10.1177/0023677219877216DOI Listing
February 2020

A safe bet? Inter-laboratory variability in behaviour-based severity assessment.

Lab Anim 2020 Feb 7;54(1):73-82. Epub 2019 Nov 7.

Institute for Laboratory Animal Science and Central Animal Facility, Hannover Medical School, Germany.

Evidence-based severity assessment is essential as a basis for ethical evaluation in animal experimentation to ensure animal welfare, legal compliance and scientific quality. To fulfil these tasks scientists, animal care and veterinary personnel need assessment tools that provide species-relevant measurements of the animals' physical and affective state. In a three-centre study inter-laboratory robustness of body weight monitoring, mouse grimace scale (MGS) and burrowing test were evaluated. Read More

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http://dx.doi.org/10.1177/0023677219881481DOI Listing
February 2020

Wheel running behaviour in group-housed female mice indicates disturbed wellbeing due to DSS colitis.

Lab Anim 2020 Feb 1;54(1):63-72. Epub 2019 Nov 1.

Institute for Laboratory Animal Science, Hannover Medical School, Germany.

Voluntary wheel running (VWR) behaviour is a sensitive indicator of disturbed wellbeing and used for the assessment of individual experimental severity levels in laboratory mice. However, monitoring individual VWR performance usually requires single housing, which itself might have a negative effect on wellbeing. In consideration of the 3Rs principle, VWR behaviour was evaluated under group-housing conditions. Read More

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http://dx.doi.org/10.1177/0023677219879455DOI Listing
February 2020

Defining body-weight reduction as a humane endpoint: a critical appraisal.

Lab Anim 2020 Feb 30;54(1):99-110. Epub 2019 Oct 30.

Rudolf-Zenker-Institute of Experimental Surgery, University Medical Center, Rostock, Germany.

In many animal experiments scientists and local authorities define a body-weight reduction of 20% or more as severe suffering and thereby as a potential parameter for humane endpoint decisions. In this study, we evaluated distinct animal experiments in multiple research facilities, and assessed whether 20% body-weight reduction is a valid humane endpoint criterion in rodents. In most experiments (restraint stress, distinct models for epilepsy, pancreatic resection, liver resection, caloric restrictive feeding and a mouse model for Dravet syndrome) the animals lost less than 20% of their original body weight. Read More

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http://dx.doi.org/10.1177/0023677219883319DOI Listing
February 2020

Semi-automated generation of pictures for the Mouse Grimace Scale: A multi-laboratory analysis (Part 2).

Lab Anim 2020 Feb 29;54(1):92-98. Epub 2019 Oct 29.

Institute for Laboratory Animal Science, RWTH Aachen University, Germany.

The Mouse Grimace Scale (MGS) is an established method for estimating pain in mice during animal studies. Recently, an improved and standardized MGS set-up and an algorithm for automated and blinded output of images for MGS evaluation were introduced. The present study evaluated the application of this standardized set-up and the robustness of the associated algorithm at four facilities in different locations and as part of varied experimental projects. Read More

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http://dx.doi.org/10.1177/0023677219881664DOI Listing
February 2020

Performance of severity parameters to detect chemotherapy-induced pain and distress in mice.

Lab Anim 2019 Oct 29:23677219883327. Epub 2019 Oct 29.

Institute for Experimental Molecular Imaging, Medical Faculty, RWTH Aachen International University, Germany.

According to European Union directive 2010/63/EU a severity classification of experimental procedures performed on laboratory animals is mandatory. This includes a prospective evaluation of all interventions performed within the experiment, as well as an assessment of the actual burden of each animal during the experiment. In this regard, the evaluation and scoring of defined criteria regarding the health state of animals could help to early identify deteriorations in animal health and facilitate the application of humane endpoints. Read More

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http://dx.doi.org/10.1177/0023677219883327DOI Listing
October 2019
2 Reads

Measurement of corticosterone in mice: a protocol for a mapping review.

Lab Anim 2020 Feb 26;54(1):26-32. Epub 2019 Oct 26.

Institute for Laboratory Animal Science, Hannover Medical School, Germany.

Severity assessment for experiments conducted with laboratory animals is still based mainly on subjective evaluations; evidence-based methods are scarce. Objective measures, amongst which determination of the concentrations of stress hormones, can be used to aid severity assessment. Short-term increases in glucocorticoid concentrations generally reflect healthy responses to stressors, but prolonged increases may indicate impaired welfare. Read More

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http://dx.doi.org/10.1177/0023677219868499DOI Listing
February 2020

Improvement of the Mouse Grimace Scale set-up for implementing a semi-automated Mouse Grimace Scale scoring (Part 1).

Lab Anim 2020 Feb 24;54(1):83-91. Epub 2019 Oct 24.

Institute for Laboratory Animal Science and Experimental Surgery, RWTH Aachen University, Germany.

The Mouse Grimace Scale (MGS) has been widely used for the noninvasive examination of distress/pain in mice. The aim of this study was to further improve its performance to generate repeatable, faster, blinded and reliable results for developing automated and standardized pictures for MGS scoring and simultaneous evaluation of up to four animals. Videos of seven C57BL/6N mice were generated in an experiment to assess pain and stress induced by repeated intraperitoneal injection of carbon tetrachloride (CCl). Read More

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http://dx.doi.org/10.1177/0023677219881655DOI Listing
February 2020

Nursing and training of pigs used in renal transplantation studies.

Lab Anim 2019 Oct 24:23677219879169. Epub 2019 Oct 24.

Department of Clinical Sciences, Swedish University of Agricultural Sciences, Sweden.

The pig is commonly used in renal transplantation studies since the porcine kidney resembles the human kidney. To meet the requirements of intense caretaking and examination without stress, a 2-week socialisation and training programme was developed. Conventional cross-breed pigs ( = 36) with high health status were trained for 15 min/day in a four-step training programme before kidney transplantation. Read More

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http://dx.doi.org/10.1177/0023677219879169DOI Listing
October 2019

Students' acceptance of e-learning approaches in Laboratory Animal Science Training.

Lab Anim 2019 Oct 20:23677219879170. Epub 2019 Oct 20.

i3S, Instituto de Investigação e Inovação em Saúde, University of Porto, Portugal.

Different online courses and training programs in Laboratory Animal Science (LAS) have emerged across Europe in recent years. E-learning appears to be a promising solution to achieve flexibility in training while meeting the quality criteria of demanding programs in short training periods. However, little is known about how students perceive e-learning in this context, and there is also a lack of specific and valid instruments to measure this perception. Read More

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http://dx.doi.org/10.1177/0023677219879170DOI Listing
October 2019

Endorsement of Animal Research: Incorporation of In Vivo Experiments (ARRIVE) Guidelines/Gold Standard Publication Checklist (GSPC) by Chinese journals: A survey of journals' instructions for authors and editors.

Lab Anim 2019 Oct 9:23677219879181. Epub 2019 Oct 9.

Evidence-Based Medicine Center, School of Basic Medical Sciences, Lanzhou University, Lanzhou, Gansu, China.

The objective was to determine the rate at which Chinese journals include Animal Research: Reporting of In Vivo Experiments (ARRIVE) Guidelines/Gold Standard Publication Checklist (GSPC) in their instructions for authors, and the awareness and recognition of editors. The survey was performed on Chinese journals. The most recent versions each journal's instructions for authors were downloaded, and the information related to the ARRIVE/GSPC was collected. Read More

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http://dx.doi.org/10.1177/0023677219879181DOI Listing
October 2019
1 Read

A novel arthritis model induced by wind, damp, cold and heat in female rats.

Lab Anim 2019 Oct 4:23677219874844. Epub 2019 Oct 4.

The Engineering Research Center of Synthetic Polypeptide Drug Discovery and Evaluation of Jiangsu Province, China Pharmaceutical University, Nanjing, Jiangsu, People's Republic of China.

Animal models are in constant development to benefit scientific research. Rheumatoid arthritis (RA) is considered a very complex disease due to its complicated pathogenesis, and patients with rheumatic disease around the world are still unable to obtain effective, simple and curable treatment. In order to obtain a clear insight into the pathogenesis of RA, a rat model was established based on the concept of syndrome in Traditional Chinese Medicine by simulating the conditions of RA as much as possible via the change in the physical conditions wind, damp, cold and heat (WDCH). Read More

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http://dx.doi.org/10.1177/0023677219874844DOI Listing
October 2019
1 Read

Systematic analysis of severity in a widely used cognitive depression model for mice.

Lab Anim 2020 Feb 1;54(1):40-49. Epub 2019 Oct 1.

RG Animal Models in Psychiatry, Department of Psychiatry and Psychotherapy, Central Institute of Mental Health, Medical Faculty Mannheim, Heidelberg University, Germany.

Animal models in psychiatric research are indispensable for insights into mechanisms of behaviour and mental disorders. Distress is an important aetiological factor in psychiatric diseases, especially depression, and is often used to mimic the human condition. Modern bioethics requires balancing scientific progress with animal welfare concerns. Read More

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http://dx.doi.org/10.1177/0023677219874831DOI Listing
February 2020

Watch out for three FELASA Working Group recommendations.

Lab Anim 2019 Oct;53(5):513

FELASA Honorary Secretary, FELASA, Eye, UK.

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http://dx.doi.org/10.1177/0023677219872667DOI Listing
October 2019

Warming and cooling device using thermoelectric Peltier elements tested on male mice.

Lab Anim 2019 Sep 25:23677219873687. Epub 2019 Sep 25.

Department of Cardiothoracic, Transplantation and Vascular Surgery, Hannover Medical School, Hannover, Germany.

Hypothermia is a treatment strategy for different clinical conditions and an essential part of cardiopulmonary bypass in complex cardiac procedures. Clinically, cooling patients is achieved via a mattress and heat exchanger integrated into a membrane oxygenator connected to a waterbed using a refrigerator system based on volatile and toxic liquids. Peltier elements are known as environmentally friendly thermoelectric generators that enable rapid warming and cooling. Read More

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http://dx.doi.org/10.1177/0023677219873687DOI Listing
September 2019
3 Reads

A cautionary note: Toxicity of polyethylene glycol 200 injected intraperitoneally into mice.

Lab Anim 2019 Sep 16:23677219873684. Epub 2019 Sep 16.

Universität Heidelberg, Medizinische Fakultät Mannheim, Mannheim, Germany.

The parenteral administration of hydrophobic substances in vivo requires the use of organic solvents to ensure sufficient solubility and avoid precipitation. Dimethyl sulfoxide is commonly used for this purpose. Based on the common assumption that polyethylene glycol (PEG) is non-toxic, our local regulatory authorities recently recommended the use of PEG instead. Read More

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http://dx.doi.org/10.1177/0023677219873684DOI Listing
September 2019

Manganese-free chow, a refined non-invasive solution to reduce gastrointestinal signal for T-weighted magnetic resonance imaging of the mouse abdomen.

Lab Anim 2019 Sep 16:23677219869363. Epub 2019 Sep 16.

Cancer Research UK/MRC Oxford Institute for Radiation Oncology, Department of Oncology, University of Oxford, Oxford, UK.

Commercial mouse chow is designed to provide a complete, nutrient-rich diet, and it can contain upwards of 100 mg/kg manganese, an essential mineral. Manganese acts as a relaxation time-shortening contrast agent for both T and T, and where standard chow is hydrated in the gastrointestinal tract, bright signals are produced when using T-weighted imaging (TWI). As a result of peristalsis, gastrointestinal hyperintensities result in temporally unstable signals, leading to image ghosting and decreased resolution from that prescribed. Read More

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http://dx.doi.org/10.1177/0023677219869363DOI Listing
September 2019

Optimising streptozotocin dosing to minimise renal toxicity and impairment of stomach emptying in male 129/Sv mice.

Lab Anim 2019 Sep 11:23677219872224. Epub 2019 Sep 11.

Department of Pharmacology, Novo Nordisk A/S, Denmark.

The streptozotocin (STZ)-induced diabetic mouse model has been extensively used as a model for diabetes and diabetic nephropathy, but it is still influenced by many off-target toxic effects and large variation in diabetes induction. Therefore, the aim of this study was to compare different STZ dosing regimens to optimise animal welfare and minimise unwanted effects of STZ measured by acute renal toxicity, impairment of stomach emptying and weight loss. Male 129/Sv mice were injected with 1 × 50, 1 × 100, 1 × 125, 1 × 150, 1 × 200, 5 × 50, 2 × 100 and 2 × 125 mg/kg STZ or vehicle and euthanized 24 hours after the last injection. Read More

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http://dx.doi.org/10.1177/0023677219872224DOI Listing
September 2019
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Zebrafish: Housing and husbandry recommendations.

Lab Anim 2020 Jun 11;54(3):213-224. Epub 2019 Sep 11.

Karolinska Institutet, Comparative Medicine, Stockholm, Sweden.

This article provides recommendations for the care of laboratory zebrafish () as part of the further implementation of Annex A to the European Convention on the protection of vertebrate animals used for experimental and other scientific purposes, EU Commission Recommendation 2007/526/EC and the fulfilment of Article 33 of EU Directive 2010/63, both concerning the housing and care of experimental animals. The recommendations provide guidance on best practices and ranges of husbandry parameters within which zebrafish welfare, as well as reproducibility of experimental procedures, are assured. Husbandry procedures found today in zebrafish facilities are numerous. Read More

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http://journals.sagepub.com/doi/10.1177/0023677219869037
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http://dx.doi.org/10.1177/0023677219869037DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7301644PMC
June 2020
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