7,320 results match your criteria Assisted Reproduction Technology


Effect of male age on pregnancy and neonatal outcomes in the first frozen-thawed embryo transfer cycles of IVF/ICSI treatment.

Andrology 2021 May 7. Epub 2021 May 7.

Department of Assisted Reproduction, First Maternity and Infant Hospital, Tongji University School of Medicine, Shanghai, China.

Background: The effect of male age on pregnancy outcomes after assisted reproductive technology (ART) treatment shown in the previous literature is controversial. In addition, the influence of male age on neonatal outcomes following ART treatment has less been investigated.

Objectives: The aim of this study was to evaluate the effect of male age on reproductive and neonatal outcomes in couples following ART treatment. Read More

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Androgen-induced gut dysbiosis disrupts glucolipid metabolism and endocrinal functions in polycystic ovary syndrome.

Microbiome 2021 May 6;9(1):101. Epub 2021 May 6.

Center for Reproductive Medicine, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200135, China.

Background: The characteristics of polycystic ovary syndrome (PCOS), a common reproductive endocrinal disorder, are high incidence, complicated aetiology and poor therapeutic effects. PCOS patients frequently exhibit gut dysbiosis; however, its roles in the regulation of metabolic and endocrinal balances in PCOS pathophysiology are not clear.

Results: In this study, gut dysbiosis was reproduced in dehydroepiandrosterone (DHEA)-induced PCOS-like rats. Read More

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A systematic review and meta-analysis on the association between ICSI and chromosome abnormalities.

Hum Reprod Update 2021 May 6. Epub 2021 May 6.

Fertility Clinic, Rigshospitalet, Copenhagen University Hospital, Copenhagen, Denmark.

Background: In the decade following the introduction of ICSI, a higher prevalence of de novo chromosome abnormalities, in particular sex chromosome and autosomal structural abnormalities, as well as inherited abnormalities was described in children conceived by ICSI compared to both naturally conceived (NC) children and children conceived by standard IVF. The explanation for the observed increase in prevalence is not clear and has been suggested to reflect parental factors (e.g. Read More

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The Inferto-Sex Syndrome (ISS): sexual dysfunction in fertility care setting and assisted reproduction.

J Endocrinol Invest 2021 May 6. Epub 2021 May 6.

Chair of Endocrinology and Medical Sexology (ENDOSEX), Department of Systems Medicine, University of Rome Tor Vergata, Via Montpellier, 1, E Tower South. Floor 4, Room E413, 00133, Rome, Italy.

Purpose: Infertility represents a peculiar social burden affecting more than 15% of couples, provoking it a real threat to the general quality of life and to the sexual health. The medicalization (diagnosis, therapy and follow up) of the lack of fertility is frequently a challenge in term of personal and couple's involvement. In particular, while the Assisted Reproductive Technology (ART) has allowed many infertile couples to achieve pregnancy, the therapeutic process faced by the couple bears a strong psychological stress that can affect the couple's quality of life, relationship and sexuality. Read More

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Evolution of the sheep industry and genetic research in the United States: opportunities for convergence in the twenty-first century.

Anim Genet 2021 May 6. Epub 2021 May 6.

National Animal Germplasm Program, United States Department of Agriculture, Agricultural Research Service, Fort Collins, CO, 80521, USA.

The continuous development and application of technology for genetic improvement is a key element for advancing sheep production in the United States. The US sheep industry has contracted over time but appears to be at a juncture where a greater utilization of technology can facilitate industry expansion to new markets and address inefficiencies in traditional production practices. Significant transformations include the increased value of lamb in relation to wool, and a downtrend in large-scale operations but a simultaneous rise in small flocks. Read More

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Pronuclear score improves prediction of embryo implantation success in ICSI cycles.

BMC Pregnancy Childbirth 2021 May 5;21(1):361. Epub 2021 May 5.

UOS Physiopathology of Human Reproduction, IRCCS Ospedale Policlinico San Martino, Largo R. Benzi, 10, 16132, Genova, Italy.

Background: In assisted reproduction technology embryo competence is routinely evaluated on morphological criteria but efficacy remains relatively low. Additional information could be obtained by evaluating pronuclear (PN) morphology. Up to now controversial results have been reported about the prognostic value of PN score. Read More

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Influence Factors of Sexual Intercourse Frequency in Infertile Couples without Sexual Dysfunction in Southwest China: A Retrospective Study.

Urol Int 2021 May 5:1-7. Epub 2021 May 5.

Department of Assisted Reproduction Center, Sichuan Academy of Medical Science & Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, China.

Introduction: The aim of this study was to investigate the sexual intercourse frequency (SIF) of infertile couples without sexual dysfunction and analyze its related influencing factors.

Materials And Methods: We retrospectively analyzed the data of a total number of 4,923 infertile couples without sexual dysfunction who received treatment in our assisted reproductive center from October 2016 to October 2018. Both partners of couples were inquired about their information of demographic statistics, occupations, lifestyles, education backgrounds, psychological characteristics, and testostrone levels of male patients. Read More

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A 24-bp indel within the sheep gene is associated with litter size.

Anim Biotechnol 2021 May 4:1-6. Epub 2021 May 4.

Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, China.

Aryl Hydrocarbon Receptor (AHR) is a member of the PER-ARNT-SIM (PAS) family, which could mediate various biological processes, for instance, the balance of the immune system, cell proliferation, differentiation, vascular tissue remodeling and reproduction ability regulation. A previous research showed that the gene exerted important functions on the pig reproduction, implying that it could serve as a candidate gene related to animal reproductive traits. Here, the aim of this work was to identify potential insertion/deletion (indel) mutations of the gene in three sheep breeds and analyze the associations between these mutations and reproductive traits. Read More

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Lysophosphatidic acid improves oocyte quality during IVM by activating the ERK1/2 pathway in cumulus cells and oocytes.

Mol Hum Reprod 2021 May 1. Epub 2021 May 1.

Assisted Reproduction Unit, Department of Obstetrics and Gynecology, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, China.

Oocyte IVM technology is an option for fertility preservation in some groups of patients, such as those with polycystic ovary syndrome, patients with ovarian hyperstimulation syndrome, and for patients with cancer. However, the developmental potential of oocytes from IVM still needs to improve. Several previous studies have reported that lysophosphatidic acid (LPA) promotes glucose metabolism, cumulus cell (CC) expansion, and oocyte nuclear maturation. Read More

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The ART of bringing extinction to a freeze - History and future of species conservation, exemplified by rhinos.

Theriogenology 2021 Apr 18;169:76-88. Epub 2021 Apr 18.

The Leibniz Institute for Zoo and Wildlife Research, Alfred-Kowalke-Straße 17, 10315, Berlin, Germany. Electronic address:

The ongoing mass extinction of animal species at an unprecedented rate is largely caused by human activities. Progressive habitat destruction and fragmentation is resulting in accelerated loss of biodiversity on a global scale. Over decades, captive breeding programs of non-domestic species were characterized by efforts to optimize species-specific husbandry, to increase studbook-based animal exchange, and to improve enclosure designs. Read More

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A comprehensive PGT-M strategy for ADPKD patients with de novo PKD1 mutations using affected embryo or gametes as proband.

J Assist Reprod Genet 2021 May 3. Epub 2021 May 3.

Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, No. 49, North Garden Road, Haidian District, Beijing, 100191, People's Republic of China.

Autosomal dominant polycystic kidney disease (ADPKD) is the most common inherited kidney disease characterized by the development of renal cysts and progression to renal failure. Preimplantation genetic testing-monogenic disease (PGT-M) is an alternative option to obtain healthy babies. However, de novo PKD1 mutation of one of the spouses or the absence of a positive family history poses a serious challenge to PGT-M. Read More

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The association between thrombophilic genes alterations and poor ovarian response in infertile women: a retrospective case-control study.

J Obstet Gynaecol 2021 May 2:1-6. Epub 2021 May 2.

Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.

This research aimed to retrospectively investigate the possible association between poor ovarian stimulation and selected thrombophilia markers in Iranian women with infertility. For this study 100 Iranian infertile women, with a history of at least three Assisted Reproduction Technology (ART) failures (50 with a poor ovarian response and 50 with a normal response), referred to Royan Institute were selected. Targeted genetic variation evaluation for Factor V G1691A, F II Prothrombin G20210A, MTHFR C677T, MTHFR A1298C was performed by PCR-RFLP followed by Sanger Sequencing. Read More

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The journey of IVF Data collection in Turkey.

J Turk Ger Gynecol Assoc 2021 May 3. Epub 2021 May 3.

Epidémiology, Research and Development, Paris, France.

Collecting and reporting data is a crucial aspect of IVF practice. During the following two decades after the first report of European IVF-monitoring Consortium (EIM) on IVF data, the number of contributing countries increased gradually reaching nearly fourty. In 2004 for the first time Turkey took part in European registry and accordingly in World registry. Read More

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Ce-based heterogeneous catalysts by partial thermal decomposition of Ce-MOFs in activation of peroxymonosulfate for the removal of organic pollutants under visible light.

Chemosphere 2021 Apr 23;280:130637. Epub 2021 Apr 23.

School of Environmental Science and Engineering, Guangdong Provincial Key Laboratory of Soil and Groundwater Pollution Control, Southern University of Science and Technology, Shenzhen, 518055, China. Electronic address:

Metal-organic framework (MOF) derivatives have drawn considerable attention for applications in various fields. In this work, spindle-shaped Ce-TCPPs were assembled by a rapid microwave-assisted hydrothermal method. After thermal treatment at low temperature under a N atmosphere, the Ce-TCPPs were partially pyrolyzed and converted to a novel CeO/N-doped carbon/Ce-TCPP nanocomposite. Read More

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[Clinical studies on acupuncture assisting fertilization-embryo transfer: a review and thoughts].

Zhen Ci Yan Jiu 2021 Apr;46(4):342-7

Institute of Integrated Traditional Chinese and Western Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.

Acupuncture has a long history of application in the field of reproduction, and in the past 20 years, numerous clinical studies have been conducted to explore the value of acupuncture in assisted reproductive technology. However, due to inconsistent research findings, there are still controversies over the clinical safety and efficacy of acupuncture in assisting fertilization-embryo transfer (IVF-ET), which may be closely associated with lacking of standardized treatment regimen in clinical trials of acupuncture. For that reason, this article reviews 16 representative clinical trials of acupuncture assisting IVF-ET published worldwide in 1999-2020, summarizes the grouping design, acupuncture method (acupuncture timing, acupuncture frequency, acupoints, and acupuncture types), and pregnancy outcome (pregnancy rate, clinical pregnancy rate, ongoing pregnancy rate, and live birth rate), and discusses several key links in clinical protocol design, so as to provide references for future clinical research. Read More

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Changes in thyroid function during controlled ovarian hyperstimulation (COH) and its impact on assisted reproduction technology (ART) outcomes: a systematic review and meta-analysis.

J Assist Reprod Genet 2021 Apr 30. Epub 2021 Apr 30.

Branch of National Clinical Research Center for Metabolic Diseases, Division of Endocrinology, Department of Internal Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, Hubei, China.

Background: To investigate the thyroid function changes during controlled ovarian hyperstimulation (COH) and ascertain its impact on reproductive outcomes.

Methods: We conducted meta-analysis in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. A comprehensive literature search was performed to identify studies reported changes in thyroid parameters during COH. Read More

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Smaller volume and altered functional connectivity of the amygdala in patients with lifelong premature ejaculation.

Eur Radiol 2021 Apr 30. Epub 2021 Apr 30.

Life Science Research Center, School of Life Science and Technology, Xidian University, Xi'an, 710071, Shaanxi, China.

Objectives: To analyze the abnormal amygdala structure and function in lifelong premature ejaculation (PE) patients compared with healthy controls (HCs).

Methods: Forty-four lifelong PE patients and thirty-one HCs were enrolled in this study. Each subject was diagnosed with PE using a Premature Ejaculation Diagnostic Tool (PEDT) and intravaginal ejaculation latency time (IELT) score. Read More

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Genetic testing of products of conception in recurrent pregnancy loss evaluation.

Reprod Biomed Online 2021 Mar 23. Epub 2021 Mar 23.

Division of Reproductive Endocrinology, Department of Obstetrics and Gynecology, Vanderbilt University Medical Center, Nashville TN, USA; Recurrent Pregnancy Loss Center, Fertility Associates of Memphis, Memphis TN, USA.

Genetic testing of products of conception (POC) has been proposed as a tool to be used in the evaluation of patients with recurrent pregnancy loss (RPL). Following a complete RPL evaluation, POC results may reveal an aneuploidy and provide an explanation for the miscarriage in more than 55% of cases. When the cytogenetic result of the pregnancy loss reveals a euploid pregnancy, management should be directed towards the identification of treatable abnormalities. Read More

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International Committee for Monitoring Assisted Reproductive Technologies (ICMART): world report on assisted reproductive technologies, 2013.

Fertil Steril 2021 Apr 26. Epub 2021 Apr 26.

Equal3 Fertility, Cupertino; Stanford University School of Medicine, Palo Alto; and University of California School of Medicine, San Francisco, California.

Objective: To report the utilization, effectiveness, and safety of practices in assisted reproductive technology (ART) globally in 2013 and assess global trends over time.

Design: Retrospective, cross-sectional survey on the utilization, effectiveness, and safety of ART procedures performed globally during 2013.

Setting: Seventy-five countries and 2,639 ART clinics. Read More

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Hot Topics on Fertility Preservation for Women and Girls-Current Research, Knowledge Gaps, and Future Possibilities.

J Clin Med 2021 Apr 13;10(8). Epub 2021 Apr 13.

Department of Oncology and Pathology, Karolinska Institutet, SE-171 64 Stockholm, Sweden.

Fertility preservation is a novel clinical discipline aiming to protect the fertility potential of young adults and children at risk of infertility. The field is evolving quickly, enriched by advances in assisted reproductive technologies and cryopreservation methods, in addition to surgical developments. The best-characterized target group for fertility preservation is the patient population diagnosed with cancer at a young age since the bulk of the data indicates that the gonadotoxicity inherent to most cancer treatments induces iatrogenic infertility. Read More

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Is frozen embryo transfer better than fresh embryo transfer in women undergoing intracytoplasmic sperm injection over the age of thirty-five? A single referral centre experience.

J Obstet Gynaecol 2021 Apr 29:1-5. Epub 2021 Apr 29.

Department of IVF Research and Training Center, Ege University School of Medicine, Izmir, Turkey.

Developments in embryo freezing techniques in recent years have increased the chance of pregnancy after frozen embryo transfers. In this study we aimed to compare the pregnancy outcomes between fresh (ET) and frozen-thawed embryo transfer (FET) and to evaluate the benefits of embryo freezing strategy for a woman of advanced age. A total of 513 ET cycles in women over the age of 35 years, including 397 fresh ET cycles and 116 FET cycles were reviewed. Read More

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A Potential Pathogenic Link Between Cancer of Female Reproductive System and Infertile Women Treated With Assisted Reproduction Techniques.

In Vivo 2021 May-Jun;35(3):1393-1399. Epub 2021 Apr 28.

Department of Obstetrics and Gynecology, Democritus University of Thrace, Komotini, Greece.

Prevention and treatment of infertility remains a priority for developed countries where a large proportion of women undergo in vitro fertilization (IVF) after ovarian stimulation. Latest data suggest that, in the USA alone, almost eight million women of fertile age will have sought medical advice for fertility problems by 2025. However, over the last years, attention has been increasingly focused, and questions have risen, on the long-term health effects in women who underwent assisted reproductive technologies (ARTs). Read More

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Single human oocyte transcriptome analysis reveals distinct maturation stage-dependent pathways impacted by age.

Aging Cell 2021 Apr 28:e13360. Epub 2021 Apr 28.

Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, Barcelona, Spain.

Female fertility is inversely correlated with maternal age due to a depletion of the oocyte pool and a reduction in oocyte developmental competence. Few studies have addressed the effect of maternal age on the human mature oocyte (MII) transcriptome, which is established during oocyte growth and maturation, however, the pathways involved remain unclear. Here, we characterize and compare the transcriptomes of a large cohort of fully grown germinal vesicle stage (GV) and in vitro matured (IVM-MII) oocytes from women of varying reproductive age. Read More

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Evaluation of follicular flushing with double lumen needle in patients undergoing assisted reproductive technology treatments.

JBRA Assist Reprod 2021 Apr 27;25(2):272-275. Epub 2021 Apr 27.

Fertipraxis, Human Reproduction Center, Rio de Janeiro, Brazil.

Objective: The purpose of this study was to investigate the possible impact of follicular flushing on the number of oocytes retrieved and oocytes in metaphase II in patients with poor ovarian response (POR) compared to direct aspiration.

Methods: This prospective, comparative, randomized single center study included 208 punctures of patients with POR, submitted to assisted reproduction technology (ART) treatments. Two groups were compared; one in which double lumen needles were used (Wallace DNS1733) for follicular flushing (n=105), and one in which single lumen needles were used (Wallace ONS1733) for direct aspiration (n=103), upon the observation of ≤ 5 follicles between 15-17 mm, ≤ 4 follicles with sizes greater than 18 mm on hCG day, and ≤ 7 recovered oocytes. Read More

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[Performance of vaginal self-sampling high-risk HPV genotyping as primary and combining cytology or viral load as secondary in cervical cancer screening].

Zhonghua Fu Chan Ke Za Zhi 2021 Apr;56(4):271-279

Department of Obstetrics and Gynecology, Peking University Shenzhen Hospital, Institute of Obstetrics and Gynecology, Shenzhen Peking University-Hong Kong University of Science and Technology Medical Center, Shenzhen Key Laboratory on Technology for Early Diagnosis of Major Gynecological Diseases, Shenzhen 518000, China.

To evaluate the efficacy of high-risk HPV (HR-HPV) genotyping with vaginal self-sampling in primary screening and combining cytology or viral load for HR-HPV positive as secondary screening strategies. The data referring to HR-HPV genotyping of self-collected sample with mass array matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF-MS), HR-HPV viral load of physician-collected sample with hybrid capture Ⅱ (HC-Ⅱ), liquid-based cytology and histology of 8 556 women were from Shenzhen cervical cancer screening trial Ⅱ (SHENCCAST-Ⅱ) conducted between April 2009 and April 2010. The data were reanalyzed to determine the sensitivity and specificity to cervical intraepithelial neoplasia (CIN) of grade 2 or worse (CIN Ⅱ), CIN of grade 3 or worse (CIN Ⅲ) when HR-HPV genotyping combining with colposcopy as primary screening strategy based on varied HR-HPV subtype (strategy 1, including 5 sub-strategies: 1a: HPV 16/18 positive; 1b: HPV 16/18/58 positive; 1c: HPV 16/18/58/31/33 positive; 1d: HPV 16/18/58/31/33/52 positive; 1e: any HR-HPV positive). Read More

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Semen evaluation: methodological advancements in sperm quality-specific fertility assessment.

Anim Biosci 2021 Apr 23. Epub 2021 Apr 23.

College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Korea.

Assessment of male fertility is based on the evaluation of sperm. Semen evaluation measures various sperm quality parameters as fertility indicators. However, semen evaluation has limitations, and it requires the advancement and application of strict quality control methods to interpret the results. Read More

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A homozygous variant in TBPL2 was identified in women with oocyte maturation defects and infertility.

Hum Reprod 2021 Apr 24. Epub 2021 Apr 24.

Center for Reproductive Medicine, Cheeloo College of Medicine, Shandong University, Key Laboratory of Reproductive Endocrinology of Ministry of Education, Shandong University, Shandong Key Laboratory of Reproductive Medicine, Jinan, China.

Study Question: What are the genetic causes of oocyte maturation defects?

Summary Answer: A homozygous splicing variant (c.788 + 3A>G) in TATA-box binding protein like 2 (TBPL2) was identified as a contributory genetic factor in oocyte maturation defects.

What Is Known Already: TBPL2, a vertebrate oocyte-specific general transcription factor, is essential for oocyte development. Read More

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Simplified sperm testing devices: a possible tool to overcome lack of accessibility and inconsistency in male factor infertility diagnosis. An opportunity for low- and middle- income countries.

Facts Views Vis Obgyn 2021 Mar 31;13(1):79-93. Epub 2021 Mar 31.

Genk Institute for Fertility Technology, Genk, Belgium.

Background: Manual semen assessment (MSA) is a key component in a male's fertility assessment. Clinicians rely on it to make diagnostic and treatment decisions. When performed manually, this routine laboratory test is prone to variability due to human intervention which can lead to misdiagnosis and consequently over- or under- treatment. Read More

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Qualitative and quantitative detection of surgical pathogenic microorganisms Escherichia coli and Staphylococcus aureus based on ddPCR system.

Sci Rep 2021 Apr 22;11(1):8771. Epub 2021 Apr 22.

Department of General Surgery, The First Hospital of Shanxi Medical University, Yingze District, No. 85 Jiefang South Road, Taiyuan, 030001, People's Republic of China.

Bacterial culture and drug susceptibility testing are used to identify pathogen infections. Nevertheless, the process requires several days from collection to the identification of bacterial species and drug-resistance patterns. The digital PCR system is a rapidly developing quantitative detection technology widely applied to molecular diagnosis, including copy number variations, single nucleotide variant analysis, cancer biomarker discovery, and pathogen identification. Read More

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