6,507 results match your criteria Assisted Reproduction Technology


Metagenomic analysis identified microbiome alterations and pathological association between intestinal microbiota and polycystic ovary syndrome.

Fertil Steril 2020 Jun;113(6):1286-1298.e4

Center for Reproductive Medicine, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, People's Republic of China; Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics, Shanghai, People's Republic of China. Electronic address:

Objective: To identify different microbial species in women with polycystic ovary syndrome (PCOS) and reveal a possible relationship between gut dysbiosis and pathological changes.

Design: Cross-sectional study.

Setting: Academic institution. Read More

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http://dx.doi.org/10.1016/j.fertnstert.2020.01.027DOI Listing

Protein Expression Profile in IVF Follicular Fluid and Pregnancy Outcome Analysis in Euthyroid Women with Thyroid Autoimmunity.

ACS Omega 2020 May 14;5(20):11439-11447. Epub 2020 May 14.

Department of Endocrinology and Metabolism, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China.

The objective of this study is to investigate the influence of the thyroid autoantibodies on the protein expression in follicular fluid and the clinical outcome of assisted reproductive technology. A total of 602 patients treated for infertility were screened; 49 euthyroid women who were positive for thyroid autoantibodies and 63 negative controls were recruited. Follicular fluid samples were analyzed using proteomics. Read More

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http://dx.doi.org/10.1021/acsomega.0c00463DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7254522PMC

Bi-allelic Missense Pathogenic Variants in TRIP13Cause Female Infertility Characterized by Oocyte Maturation Arrest.

Am J Hum Genet 2020 May 22. Epub 2020 May 22.

Institute of Pediatrics, Children's Hospital of Fudan University, the Shanghai Key Laboratory of Medical Epigenetics, the International Co-laboratory of Medical Epigenetics and Metabolism, the Ministry of Science and Technology, the Institutes of Biomedical Sciences, and the State Key Laboratory of Genetic Engineering, Fudan University, Shanghai 200032, China; Shanghai Center for Women and Children's Health, Shanghai 200062, China. Electronic address:

Normal oocyte meiosis is a prerequisite for successful human reproduction, and abnormalities in the process will result in infertility. In 2016, we identified mutations in TUBB8 as responsible for human oocyte meiotic arrest. However, the underlying genetic factors for most affected individuals remain unknown. Read More

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http://dx.doi.org/10.1016/j.ajhg.2020.05.001DOI Listing

Transiently increased risk of breast cancer after childbirth: implications for fertility treatments and surrogacy.

Hum Reprod 2020 May 30. Epub 2020 May 30.

Warren Alpert Medical School of Brown University, Providence, RI, USA.

Whereas longstanding dogma has purported that pregnancies protect women from breast cancer, a recent meta-analysis now mandates reconsideration since it reported an actual higher breast cancer risk for more than two decades after childbirth before the relative risk turns negative. Moreover, the risk of breast cancer appears higher for women having their first birth at an older age and with a family history and it is not reduced by breastfeeding. The process of obtaining informed consent for all fertility treatments, therefore, must make patients aware of the facts that every pregnancy, to a small degree, will increase the short-term breast cancer risk. Read More

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http://dx.doi.org/10.1093/humrep/deaa102DOI Listing

A blastocyst biopsy approach for preimplantation genetic diagnosis technique that affects the expression of SNAP-α in mice.

Reprod Biol 2020 May 26. Epub 2020 May 26.

Key Laboratory of Reproductive Medicine of Guangdong Province, the First Affiliated Hospital and School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, China. Electronic address:

Preimplantation genetic diagnosis (PGD) is a technique that is commonly used during assisted reproduction in the clinics to eliminate genetically abnormal embryos before implantation. The blastomere biopsy technique has risks related to the embryo, but blastocyst biopsy has not been systematically evaluated in relation to effects after birth, and the resulting offspring have not been followed up on. We designed a series of experiments to evaluate the risk of blastocyst biopsy on the resulting progeny. Read More

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http://dx.doi.org/10.1016/j.repbio.2020.03.010DOI Listing

Minor DNA methylation changes are observed in spermatozoa prepared using different protocols.

Andrology 2020 May 29. Epub 2020 May 29.

Department of Human Reproduction, Division of Gynaecology, University Medical Center Ljubljana, Ljubljana, Slovenia.

Background: DNA methylation patterns can show transgenerational inheritance and are influenced by lifestyle and environmental factors. It is suggested that these patterns can be changed by assisted reproductive technology.

Objectives: To evaluate the impact of two different sperm preparation methods, conventional density gradient centrifugation (DGC) vs. Read More

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http://dx.doi.org/10.1111/andr.12832DOI Listing

The live birth and neonatal outcomes in the subsequent pregnancy among patients with adverse pregnancy outcomes in first frozen embryo transfer cycles.

Arch Gynecol Obstet 2020 May 28. Epub 2020 May 28.

Department of Assisted Reproduction, Shanghai Ninth People's Hospital affiliated to JiaoTong University School of Medicine, Zhizaoju Road No. 639, Shanghai, China.

Purpose: To explore whether the adverse pregnancy outcomes in first frozen embryo transfer (FET) cycles affect live birth and neonatal outcomes in the subsequent pregnancy?

Methods: This was a retrospective study. Women with a history of adverse pregnancy outcomes in first FET cycles started their subsequent embryo transfer cycles. The adverse pregnancy outcomes included biochemical pregnancy, ectopic pregnancy, and first-trimester pregnancy loss. Read More

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http://dx.doi.org/10.1007/s00404-020-05608-0DOI Listing

[Analysis of prognosis and pregnancy outcomes of fertility-preserving treatment for patients with stage Ⅰa, grade 2 endometrial cancer].

Zhonghua Fu Chan Ke Za Zhi 2020 May;55(5):327-332

Department of Obstetrics and Gynecology, Peking University People's Hospital, Beijing 100044, China.

To investigate the efficacy and pregnancy outcome of fertility-preserving treatment for patients with stage Ⅰa, grade 2 endometrial cancer (EC). Clinical data was retrospectively collected for EC or atypical endometrial hyperplasia (AEH) patients treated in Peking University People's Hospital, Foshan First People's Hospital of Guangdong Province and First Affiliated Hospital of Sun Yat-sen University, from 2010 to 2019. Inclusion criteria for fertility-preserving treatment included: (1) Age ≤45 years. Read More

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http://dx.doi.org/10.3760/cma.j.cn112141-20200118-00047DOI Listing

Assisted reproductive techniques in Latin America: The Latin American Registry, 2017.

JBRA Assist Reprod 2020 May 13. Epub 2020 May 13.

Latin American Network of Assisted Reproduction (REDLARA), Montevideo, Uruguay.

Research Question: What was the utilization, effectiveness and safety of assisted reproductive techniques performed in Latin America during 2017.

Design: Retrospective collection of multinational data on ART performed in 188 institutions from 15 Latin American countries.

Results: We are reporting 93,600 initiated cycles, 16,976 deliveries and the birth of 20,404 babies. Read More

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http://dx.doi.org/10.5935/1518-0557.20200029DOI Listing

Metabolic changes in follicular fluids of patients treated with recombinant versus urinary human chorionic gonadotropin for triggering ovulation in assisted reproductive technologies: a metabolomics pilot study.

Arch Gynecol Obstet 2020 May 27. Epub 2020 May 27.

Department of Sciences, University of Basilicata, viale Ateneo Lucano 10, 85100, Potenza, Italy.

Introduction: The main goal of this retrospective cohort study is the assessment of the effects of administration of recombinant-hCG (r-hCG) versus urinary-hCG (u-hCG) on follicular fluid (FF) composition of women who underwent in vitro fertilization (IVF) treatments.

Materials And Methods: We selected 70 patients with infertility attributable to tubal diseases, unexplained infertility, and male factor. Metabolomics analysis of their FFs was performed by H nuclear magnetic resonance (H NMR) spectroscopy in combination with multivariate analysis to interpret the spectral data. Read More

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http://dx.doi.org/10.1007/s00404-020-05609-zDOI Listing

The Success Rate and Factors Affecting the Outcome of Assisted Reproductive Treatment in Subfertile Men.

Iran J Public Health 2020 Feb;49(2):332-340

Reproductive Biotechnology Research Center, Avicenna Research Institute, Tehran, Iran.

Background: This study was conducted to evaluate the success rate of male infertility treatment and the factors affecting its outcome.

Methods: In a historical cohort study, from Mar 2013 to Mar 2014, 323 couples with male factor were investigated. Couples had treated with IUI or/and ICSI were included randomly. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7231713PMC
February 2020

A randomised trial comparing conventional semen parameters, sperm DNA fragmentation levels and satisfaction levels between semen collection at home and at the clinic.

Andrologia 2020 May 26:e13628. Epub 2020 May 26.

Center of Assisted Reproduction and Embryology, The University of Hong Kong - Shenzhen Hospital, Shenzhen, China.

The aim of the randomised trial was to compare conventional semen parameters, sperm DNA fragmentation levels and satisfaction levels between semen samples collected at home and at the clinic. We recruited 110 men with a history of infertility for at least 1 year from the outpatient andrology clinic. Each man collected two semen samples, one at home and one at the clinic. Read More

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http://dx.doi.org/10.1111/and.13628DOI Listing

Effectiveness of a therapeutic multiple-lifestyle intervention taking into account the periconceptional environment in the management of infertile couples: study design of a randomized controlled trial - the PEPCI study.

BMC Pregnancy Childbirth 2020 May 26;20(1):322. Epub 2020 May 26.

Saint Antoine Research Center, INSERM équipe Lipodystrophies génétiques et acquises, Service de biologie de la reproduction-CECOS, AP-HP, Hôpital Tenon, Sorbonne Université, 4 rue de la Chine, F-75020, Paris, France.

Background: Infertility is defined as the inability to conceive after 12 months of unprotected intercourse. It affects approximately one in six couples seeking pregnancy in France or western countries. Many lifestyle factors of the couples' pre and peri-conceptional environment (weight, diet, alcohol, tobacco, coffee, drugs, physical activity, stress, sleep…) have been identified as risk factors for infertility in both males and females. Read More

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http://dx.doi.org/10.1186/s12884-020-2855-9DOI Listing

Current Therapeutic Options for Controlled Ovarian Stimulation in Assisted Reproductive Technology.

Drugs 2020 May 24. Epub 2020 May 24.

Department of Reproductive Medicine, Dexeus University Hospital, Barcelona, Spain.

Assisted reproduction technologies have substantially advanced since the birth of the first in vitro fertilization baby, and innovations within in vitro fertilization laboratories have been of paramount importance for the overall assisted reproduction technology success rates. However, one of the milestones in the history of in vitro fertilization is irrefutably the introduction of conventional ovarian stimulation. The objective of the present review is to provide an update on conventional ovarian stimulation, by giving an overview of treatment milestones, together with the latest innovations currently being investigated. Read More

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http://dx.doi.org/10.1007/s40265-020-01324-wDOI Listing

ART utilization: an indicator of access to infertility care.

Reprod Biomed Online 2020 Mar 14. Epub 2020 Mar 14.

Clinica las Condes and Program of Ethics and Public Policies in Human Reproduction, University Diego Portales, Santiago, Chile.

This commentary outlines the importance of utilizing assisted reproductive technology (ART) as an indicator of access to infertility care and provides a standard way of reporting utilization to facilitate international comparisons. Factors that influence ART utilization as well as underlying inequalities and inequities in access to care are discussed. The relevance of a marker that can inform and evaluate policy initiatives, monitor progress and document change is emphasized. Read More

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http://dx.doi.org/10.1016/j.rbmo.2020.03.007DOI Listing

Identification of a 6-month-old baby with a combination of WAGR and Potocki-Shaffer contiguous deletion syndromes by SNP array testing.

Hereditas 2020 May 23;157(1):23. Epub 2020 May 23.

Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, 100191, China.

WAGR 11p13 deletion syndrome is associated with abnormalities including (W) ilms tumor, (A) niridia, (G) enitourinary abnormalities, and growth and mental (R) etardation (WAGR). Potocki-Schaffer syndrome is a contiguous gene syndrome associated with deletions in 11p11.2, principal features of which are multiple exostoses, parietal foramina development delay, mental retardation, and facial dysmorphism. Read More

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http://dx.doi.org/10.1186/s41065-020-00132-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7245943PMC

Human Chorionic Gonadotropin Priming Does Not Improve Pregnancy Outcomes of PCOS-IVM Cycles.

Front Endocrinol (Lausanne) 2020 30;11:279. Epub 2020 Apr 30.

Department of Obstetrics and Gynecology, Center for Reproductive Medicine, Peking University Third Hospital, Beijing, China.

Influence of pre-retrieval human chorionic gonadotropin (HCG) priming on outcomes of maturation (IVM) remains controversial. This study aimed to evaluate the effect of HCG priming before oocyte retrieval on clinical outcomes of IVM cycles in patients with polycystic ovarian syndrome (PCOS). This was a retrospective cohort study analyzing data from the first IVM cycles of unstimulated PCOS patients in a reproductive center of university affiliated hospital from January 2006 to December 2017. Read More

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http://dx.doi.org/10.3389/fendo.2020.00279DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7204525PMC

Body mass index (BMI) is associated with serum thyroid-stimulating hormone (TSH) level in infertile women: a cross-sectional study.

Endocr J 2020 May 15. Epub 2020 May 15.

Center for Reproductive Medicine, Affiliated Hospital of Jining Medical University, Jining, Shandong 272029, China.

Studies suggest that there is a relationship between body mass index (BMI) and thyroid-stimulating hormone (TSH) levels. But conflicting evidence exists regarding the relationship between the two variables. Moreover, thyroid function is closely related to female fertility and has certain effects on infertility. Read More

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http://dx.doi.org/10.1507/endocrj.EJ19-0441DOI Listing

Assisted reproductive technologies in Latin America: The Latin American Registry, 2017.

Reprod Biomed Online 2020 Feb 20. Epub 2020 Feb 20.

Latin American Network of Assisted Reproduction (REDLARA), Plaza Independencia 811 Montevideo, Uruguay.

Research Question: What was the utilization, effectiveness and safety of assisted reproductive technologies (ART) carried out in Latin America during 2017.

Design: Retrospective collection of multinational data on ART carried out in 188 institutions from 15 Latin American countries.

Results: In this study, 93,600 initiated cycles, 16,976 deliveries and 20,404 births reported. Read More

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http://dx.doi.org/10.1016/j.rbmo.2020.02.004DOI Listing
February 2020

Randomized, assessor-blinded trial comparing highly purified human menotropin and recombinant follicle-stimulating hormone in high responders undergoing intracytoplasmic sperm injection.

Fertil Steril 2020 May 13. Epub 2020 May 13.

Ferring Pharmaceuticals, Inc., Parsippany, New Jersey. Electronic address:

Objective: To evaluate the efficacy and safety of highly purified human menotropin (HP-hMG) and recombinant follicle-stimulating hormone (rFSH) for controlled ovarian stimulation in a population of patients predicted to be high responders.

Design: Randomized, open-label, assessor-blinded, parallel-group, noninferiority trial.

Setting: Fertility centers. Read More

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http://dx.doi.org/10.1016/j.fertnstert.2020.03.029DOI Listing

Long-term storage of boar seminal doses contaminated with Proteus vulgaris: A dose-dependent effect on sperm motility and sperm-bacteria interaction.

Anim Reprod Sci 2020 May 9;216:106349. Epub 2020 Apr 9.

Biotechnology of Animal and Human Reproduction (TechnoSperm), Unit of Cell Biology, Department of Biology, Institute of Food and Agricultural Technology, Faculty of Sciences, University of Girona, C/Maria Aurèlia Campany, 69, Campus de Montilivi, 17003, Girona, Spain. Electronic address:

This study evaluated how Proteus vulgaris affects sperm quality and sperm-bacteria interaction in stored semen samples. A strain of P. vulgaris resistant to streptomycin, penicillin, lincomycin and spectinomycin was added to boar semen in doses of 10, 10, 10, 10 and 10 CFU/mL. Read More

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http://dx.doi.org/10.1016/j.anireprosci.2020.106349DOI Listing

Effects of selection by the Percoll density gradient method on motility, mitochondrial membrane potential and fertility in a subpopulation of Atlantic salmon (Salmo salar) testicular spermatozoa.

Anim Reprod Sci 2020 May 2;216:106344. Epub 2020 Apr 2.

Nucleus of Research in Food Production, Faculty of Natural Resources, Catholic University of Temuco, Temuco, Chile. Electronic address:

The aim of this study was to evaluate effects of selection using the Percoll density gradient method on motility, mitochondrial membrane potential (ΔΨMit) and fertility in a subpopulation of testicular spermatozoa obtained from Atlantic salmon (Salmo salar). Samples were divided into three groups: Control (C), T1 (45/90 % Percoll®) and T2 (45/60 % Percoll®). Sperm motility was evaluated using CASA (Computer-Assisted Sperm Analysis), ΔΨMit using flow cytometry, and fertility evaluating whether cleavage of fertilised eggs had occurred after 16 h of incubation at 10 °C. Read More

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http://dx.doi.org/10.1016/j.anireprosci.2020.106344DOI Listing

Integrative analysis of genomic and epigenetic regulation of endometrial cancer.

Aging (Albany NY) 2020 May 15;12. Epub 2020 May 15.

Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100191, China.

Endometrial carcinomas (EC) are characterized by high DNA copy numbers and DNA methylation aberrations. In this study, we sought to comprehensively explore the effect of these two factors on development and progression of EC by analyzing integrated genomic and epigenetic analysis to. We found high DNA copy number and DNA methylation abnormalities in EC, with 6308 copy-number variation genes (CNV-G) and 4376 methylation genes (MET-G). Read More

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http://dx.doi.org/10.18632/aging.103202DOI Listing

Effect of serum 25-hydroxyvitamin D levels on sperm quality and assisted reproductive technology outcomes for men of infertile Chinese couples.

Andrology 2020 May 15. Epub 2020 May 15.

Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, 100191, China.

Introduction: Vitamin D insufficiency/deficiency is highly prevalent in China, but its effect on sperm quality and outcomes of assisted reproductive technology (ART) in men from infertile couples is not clear. This study aimed to explore the association of serum 25-hydroxyvitamin D (25OHD) levels and semen parameters, as well as ART outcomes for men from infertile couples.

Materials And Methods: This study recruited 1,308 men from infertile couples who attended the Reproductive Medicine Center of Peking University Third Hospital for first time from February to August 2019(NCT03804294). Read More

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http://dx.doi.org/10.1111/andr.12818DOI Listing

Australia after Cresswell and Chapman: A Legal and Regulatory Paradox, or an Opportunity for Uniformity?

J Law Med 2020 Apr;27(3):741-761

Consultant Physician, West Gippsland Health Care Group; Interventional Endoscopy Fellow, Austin Health; Juris Doctor Student, Flinders University of South Australia; Masters of Health and Medical Law Student, University of Melbourne; Liver Transplant Unit, Austin Health, Heidelberg, Victoria.

Australia, like many overseas jurisdictions, has recognised the need to cope with advancing medical technology and changing community attitudes towards assisted reproductive technology (ART). Despite this, several States in Australia still do not have legislative instruments regulating ART, and those that have legislated have done so in a non-uniform way. In 2018/2019, four cases came before State Supreme Courts, where the female applicants had to endure significant legal battle in order to utilise their late partner's gametes, highlighting the inability of the law to provide an appropriate clinical framework. Read More

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COVID-19 and assisted reproductive technology services: repercussions for patients and proposal for individualized clinical management.

Reprod Biol Endocrinol 2020 May 13;18(1):45. Epub 2020 May 13.

Fertility Clinic Skive, Skive Regional Hospital, Skive, Denmark.

The prolonged lockdown of health services providing high-complexity fertility treatments -as currently recommended by many reproductive medicine entities- is detrimental for society as a whole, and infertility patients in particular. Globally, approximately 0.3% of all infants born every year are conceived using assisted reproductive technology (ART) treatments. Read More

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http://dx.doi.org/10.1186/s12958-020-00605-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7218705PMC

Erythropoietin-producing hepatocellular A7 restrains estrogen-negative feedback of luteinizing hormone via ephrin A5 in the hypothalamus of female rats.

Am J Physiol Endocrinol Metab 2020 May 12. Epub 2020 May 12.

Center for Reproductive Medicine, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200135, China; Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics, Shanghai 200135, China.

We have previously shown that systemic injection of exogenous erythropoietin-producing hepatocellular (EPH) A7-Fc raises serum luteinizing hormone (LH) levels before ovulation in female rats, indicating the induction of EPHA7 in ovulation. In this study, we aimed to identify the mechanism and hypothalamus-pituitary-ovary (HPO) axis level underlying the promotion of LH secretion by EPHA7. Using an ovariectomized (OVX) rat model, in conjunction with low-dose estradiol (E) treatment, we investigated the association between EPHA7-ephrin (EFN) A5 signaling and E-negative feedback. Read More

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http://dx.doi.org/10.1152/ajpendo.00046.2020DOI Listing

Imprinting disorders in children born after ART: a Nordic study from the CoNARTaS group.

Hum Reprod 2020 May;35(5):1178-1184

Gynecological Clinic, Rigshospitalet, University of Copenhagen, 2100 Copenhagen, Denmark.

Study Question: Is the risk of imprinting disorders increased in children conceived after ART?

Summary Answer: We found an adjusted odds ratio (AOR) of 2.84 [95% CI: 1.34-6. Read More

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http://dx.doi.org/10.1093/humrep/deaa039DOI Listing

Paternal age, body mass index, and semen volume are associated with chromosomal aberrations-related miscarriages in couples that underwent treatment by assisted reproductive technology.

Aging (Albany NY) 2020 May 8;12(9):8459-8472. Epub 2020 May 8.

Center for Reproductive Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.

We investigated the effects of paternal characteristics, including age, body mass index (BMI), and semen parameters on chromosomal aberration-related miscarriages in couples that underwent treatment with assisted reproductive technology (ART). Single nucleotide polymorphism (SNP) array analysis showed chromosomal aberrations in 60.2% (557/925) of miscarried fetuses, including trisomy in 73. Read More

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http://dx.doi.org/10.18632/aging.103151DOI Listing

Developmental Plasticity in Response to Embryo Cryopreservation: The Importance of the Vitrification Device in Rabbits.

Animals (Basel) 2020 May 6;10(5). Epub 2020 May 6.

Instituto de Ciencia y Tecnología Animal, Universitat Politècnica de València, 46022 Valencia, Spain.

In this study, we evaluated the effect of embryo vitrification using two different devices on adulthood phenotype in rabbits. In vitro development, prenatal embryo survival, body weight, growth performance, haematological and biochemical peripheral blood analysis, reproductive performance, and lactation performance traits were compared between the experimental groups. They derived from naturally-conceived embryos (NC), fresh-transferred embryos (FT), vitrified-transferred embryos using mini-straw (VTs), or vitrified-transferred embryos using Cryotop (VTc). Read More

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http://dx.doi.org/10.3390/ani10050804DOI Listing

Desire for parenthood at the time of COVID-19 pandemic: an insight into the Italian situation.

J Psychosom Obstet Gynaecol 2020 May 7:1-8. Epub 2020 May 7.

Assisted Reproductive Technology Centre, Careggi Hospital, University of Florence, Florence, Italy.

To evaluate the impact of the COVID-19 pandemic lifestyle change on couples of reproductive age and on their desire for parenthood. A quantitative correlational research study, based on a web survey, was conducted among Italian men and women in heterosexual stable relationships, aged between 18 and 46 years. The self-administered Italian version questionnaire was created using Google Forms and posted on chats and social networks. Read More

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http://dx.doi.org/10.1080/0167482X.2020.1759545DOI Listing

The effect of SNP rs400827589 in exon 2 of the MTNR1B gene on reproductive seasonality and litter size in sheep.

Vet Med Sci 2020 May 6. Epub 2020 May 6.

Key Laboratory of Animal Genetics and Breeding and Reproduction of Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China.

In mammals, the melatonin receptor gene has been widely studied since it has a great influence on reproductive traits. However, little is known about the association between polymorphism of the coding region of the MTNR1B gene and year-round oestrus or the litter size in Small Tail Han sheep. To better understand the effects of single nucleotide polymorphism (SNP) rs400827589 in MTNR1B, a population polymorphism analysis was conducted using genotyping data in 45 sheep breeds around the world. Read More

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http://dx.doi.org/10.1002/vms3.280DOI Listing

Prospects for FSH Treatment of Male Infertility.

J Clin Endocrinol Metab 2020 Jul;105(7)

Department of Biomedical, Metabolic and Neural Sciences, University of Modena and Reggio Emilia, Modena, Italy.

Context: Despite the new opportunities provided by assisted reproductive technology (ART), male infertility treatment is far from being optimized. One possibility, based on pathophysiological evidence, is to stimulate spermatogenesis with gonadotropins.

Evidence Acquisition: We conducted a comprehensive systematic PubMed literature review, up to January 2020, of studies evaluating the genetic basis of follicle-stimulating hormone (FSH) action, the role of FSH in spermatogenesis, and the effects of its administration in male infertility. Read More

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http://dx.doi.org/10.1210/clinem/dgaa243DOI Listing

Child welfare assessments and the regulation of access to publicly funded fertility treatment.

Authors:
Judith Lind

Reprod Biomed Soc Online 2020 Jun 22;10:19-27. Epub 2020 Feb 22.

Department of Thematic Studies - Child Studies, Linköping University, Linköping, Sweden.

Assessment of the psychological and social circumstances of candidates for assisted reproduction is commonly justified with references to the welfare of the intended child. In nine focus group discussions with 64 clinic staff at four public fertility clinics in Sweden, the responsible use of public resources constituted another important justification for such assessments. Theoretically, this study draws on the identification of the role of regulatory conversations in decision makers' policy interpretations. Read More

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http://dx.doi.org/10.1016/j.rbms.2020.01.003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7191644PMC

Metformin inhibits testosterone-induced endoplasmic reticulum stress in ovarian granulosa cells via inactivation of p38 MAPK.

Hum Reprod 2020 May;35(5):1145-1158

Assisted Reproduction Unit, Department of Obstetrics and Gynecology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, 310016, Hangzhou, China.

Study Question: Does metformin inhibit excessive androgen-induced endoplasmic reticulum (ER) stress in mouse granulosa cells (GCs) in vivo and in vitro?

Summary Answer: Metformin inhibits testosterone-induced ER stress and unfolded protein response (UPR) activation by suppressing p38 MAPK phosphorylation in ovarian GCs.

What Is Known Already: Polycystic ovary syndrome (PCOS) is associated with hyperandrogenism. Excessive testosterone induces ER stress and UPR activation in human cumulus cells, leading to cell apoptosis. Read More

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http://dx.doi.org/10.1093/humrep/deaa077DOI Listing

Single-cell transcriptome analysis of the novel coronavirus (SARS-CoV-2) associated gene ACE2 expression in normal and non-obstructive azoospermia (NOA) human male testes.

Sci China Life Sci 2020 Apr 30. Epub 2020 Apr 30.

Beijing Advanced Innovation Center for Genomics, Department of Obstetrics and Gynecology, Third Hospital, College of Life Sciences, Peking University, Beijing, 100871, China.

Being infected by SARS-CoV-2 may cause damage to multiple organs in patients, such as the lung, liver and heart. Angiotensin-converting enzyme 2 (ACE2), reported as a SARS-CoV-2 receptor, is also expressed in human male testes. This suggests a potential risk in human male reproductive system. Read More

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http://dx.doi.org/10.1007/s11427-020-1705-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7195615PMC

Internet Influence of Assisted Reproduction Technology Centers in China: A Model Based on WeChat Official Accounts Measurement.

J Med Internet Res 2020 Feb 26. Epub 2020 Feb 26.

Department of Histology and Embryology, Nanjing Medical University, Nanjing, CN.

Background: The prevalence of infertility in China is high, but the advent of assisted reproduction technology (ART) has greatly eased this situation. Social media, such as WeChat official accounts, have become the preferred tool for ART centers to communicate with patients, but their attention and operational status differ, and the internet influence of WeChat official accounts is insufficient. In addition, questions about whether internet influence is consistent with academic influence and whether the internet can influence patients' choice of medical treatment to a certain extent have not been explored. Read More

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http://dx.doi.org/10.2196/17997DOI Listing
February 2020

After corona: there is life after the pandemic.

Authors:
Jan Tesarik

Reprod Biomed Online 2020 06 8;40(6):760-762. Epub 2020 Apr 8.

Molecular Assisted Reproduction and Genetics, MAR&Gen Clinic, Camino de Ronda 2, Granada 18006, Spain. Electronic address:

The current pandemic of Coronavirus Disease 2019 (COVID-19) has focused the attention of medical-care providers away from non-life-threatening diseases, including infertility. Although infertility does not jeopardize the physical survival of infertile couples, it does jeopardize their future quality of life. Human infertility can be caused by a number of factors, some of which are age-dependent, and their effects may become irreversible if appropriate measures are not taken in time to prevent irreversible childlessness. Read More

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http://dx.doi.org/10.1016/j.rbmo.2020.04.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7141638PMC
June 2020
3.015 Impact Factor

Novel PGD strategy based on single sperm linkage analysis for carriers of single gene pathogenic variant and chromosome reciprocal translocation.

J Assist Reprod Genet 2020 May 29;37(5):1239-1250. Epub 2020 Apr 29.

Department of Obstetrics and Gynecology, Center for Reproductive Medicine, Peking University Third Hospital, Beijing, 100191, China.

Purpose: Preimplantation genetic diagnosis (PGD) analysis can be challenging for couples who carry more than one genetic condition. In this study, we describe a new PGD strategy to select which embryo(s) to transfer for two clinically challenging cases. Both cases lack essential family members for linkage analysis including de novo mutation combined with reciprocal translocation. Read More

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http://dx.doi.org/10.1007/s10815-020-01753-2DOI Listing

Sex determination in the GIFT strain of tilapia is controlled by a locus in linkage group 23.

BMC Genet 2020 Apr 29;21(1):49. Epub 2020 Apr 29.

Institute of Aquaculture, Faculty of Natural Sciences, University of Stirling, Stirling, Scotland, UK.

Background: Tilapias (Family Cichlidae) are the second most important group of aquaculture species in the world. They have been the subject of much research on sex determination due to problems caused by early maturation in culture and their complex sex-determining systems. Different sex-determining loci (linkage group 1, 20 and 23) have been detected in various tilapia stocks. Read More

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http://dx.doi.org/10.1186/s12863-020-00853-3DOI Listing

Microsurgical gonadal-inferior epigastric vein anastomosis to treat the nutcracker phenomenon with left gonadal vein varices with reflux.

Int Urol Nephrol 2020 Apr 27. Epub 2020 Apr 27.

Department of Urology, Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu, 611731, China.

Objective: To evaluate the effectiveness and safety of microsurgical gonadal-inferior epigastric vein anastomosis for the treatment of the nutcracker phenomenon (NCP) associated with left gonadal vein varices with reflux.

Methods: Thirty-five patients with NCP associated with left gonadal vein varices with reflux diagnosed in our hospital from June 2016 to June 2018 were included. All patients underwent a shunt operation consisting of microsurgical gonadal-inferior epigastric vein anastomosis, and the patients were followed up for 1 year. Read More

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http://dx.doi.org/10.1007/s11255-020-02478-yDOI Listing
April 2020
1.293 Impact Factor

Decreased brain and muscle ARNT-like protein 1 expression mediated the contribution of hyperandrogenism to insulin resistance in polycystic ovary syndrome.

Reprod Biol Endocrinol 2020 Apr 25;18(1):32. Epub 2020 Apr 25.

Center for Reproductive Medicine, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, 845 Lingshan Road, Shanghai, 200135, China.

Background: The interface between environmental risk factors and genetic factors could contribute to the pathogenesis of hyperandrogenism and insulin resistance in polycystic ovary syndrome (PCOS); however, the underlying complex mechanism remains to be elucidated.

Methods: We used dehydroepiandrosterone (DHEA)-induced PCOS-like rat model to measure circadian clock genes and insulin resistance-related genes. Additionally, we performed in vitro experiments in mature adipocytes to verify the molecular mechanisms. Read More

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http://dx.doi.org/10.1186/s12958-020-00592-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7183135PMC

Rare mutations in the autophagy-regulating gene AMBRA1 contribute to human neural tube defects.

Hum Mutat 2020 Apr 25. Epub 2020 Apr 25.

Obstetrics and Gynecology Hospital, State Key Laboratory of Genetic Engineering at School of Life Sciences, Fudan University, Shanghai, China.

Neural tube defects (NTDs) are severe congenital malformations caused by failed neural tube closure. Recently, autophagy is revealed to play a vital role in neuroepithelium development and neurulation. Autophagy and beclin 1 regulator 1 (Ambra1) is a crucial regulator of autophagy initiation, and its deficiency in mice leads to exencephaly and/or spina bifida. Read More

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http://dx.doi.org/10.1002/humu.24028DOI Listing

Progestins versus GnRH analogues for pituitary suppression during ovarian stimulation for assisted reproductive technology: a systematic review and meta-analysis.

Reprod Biomed Online 2020 Jun 5;40(6):894-903. Epub 2020 Feb 5.

Department of Obstetrics and Gynecology, Koc University Hospital, Davutpaşa Cad. No: 4 Topkapi, Istanbul 34010, Turkey; Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Koc University School of Medicine, Rumelifeneri Yolu, 34450, Sarıyer, Istanbul, Turkey. Electronic address:

This systematic review and meta-analysis of comparative studies investigated whether progestins are as effective as gonadotrophin releasing hormone (GnRH) analogues for pituitary suppression in assisted reproduction. The primary outcome was live birth rate per woman. Secondary outcomes were live birth or ongoing pregnancy per woman and per embryo transfer, ongoing pregnancy, clinical pregnancy, numbers of oocytes and metaphase-two oocytes, duration of stimulation and gonadotrophin consumption. Read More

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http://dx.doi.org/10.1016/j.rbmo.2020.01.027DOI Listing

Likelihood of live birth with extremely low β-hCG level 14 days after fresh embryo transfer.

Gynecol Endocrinol 2020 Apr 23:1-6. Epub 2020 Apr 23.

Department of Reproductive Medicine, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, China.

To investigate the pregnancy outcomes of patients with low serum β-hCG level 14 days after day 3 embryo transfer. A retrospective study was performed with 723 patients with a serum β-hCG level between 5 and 100 mIU/ml 14 days after embryo transfer. Pregnancy outcomes (live birth, clinical pregnancy) were analyzed according to the female patients' age. Read More

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http://dx.doi.org/10.1080/09513590.2020.1754787DOI Listing

A novel mathematical model of true ovarian reserve assessment based on predicted probability of poor ovarian response: a retrospective cohort study.

J Assist Reprod Genet 2020 Apr 21;37(4):963-972. Epub 2020 Apr 21.

Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, 49 Huayuan North Road, Haidian District, Beijing, 100191, People's Republic of China.

Purpose: To establish a mathematical model for assessing the true ovarian reserve based on the predicted probability of poor ovarian response (POR).

Methods: In this retrospective cohort study, a total of 1523 GnRH-antagonist cycles in 2017 were firstly analyzed. The ovarian responses were calculated based on the number of retrieved oocytes. Read More

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http://dx.doi.org/10.1007/s10815-020-01700-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7183040PMC

Live birth in women with multiple sclerosis receiving assisted reproduction.

Reprod Biomed Online 2020 May 27;40(5):711-718. Epub 2020 Jan 27.

Centre for Clinical Epidemiology, Odense University Hospital, Odense, Denmark and Research Unit of Clinical Epidemiology, Department of Clinical Research, University of Southern Denmark, Kløvervænget 30, Entrance 216, Odense C DK-5000, Denmark.

Research Question: Multiple sclerosis is predominant in women during the childbearing years and, in case of problems with conceiving naturally, assisted reproductive technology (ART) becomes relevant. The efficacy of ART treatment in women with multiple sclerosis has not previously been studied. Our aim was to examine the chance of a liveborn infant after an embryo transfer in women with multiple sclerosis and to examine whether corticosteroids before embryo transfer had a beneficial effect. Read More

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http://dx.doi.org/10.1016/j.rbmo.2020.01.013DOI Listing

Preimplantation loss of fertilized human ova: estimating the unobservable.

Hum Reprod 2020 Apr;35(4):743-750

Dept. Obstetrics and Gynecology, Brown University, Providence, RI, USA.

Study Question: What proportion of fertilized human ova are lost before implantation?

Summary Answer: An estimated 40 to 50% of fertilized ova fail to implant.

What Is Known Already: Preimplantation loss is not detectable with current technology. Published estimates of preimplantation loss range from 10 to 70%. Read More

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http://dx.doi.org/10.1093/humrep/deaa048DOI Listing

A novel 23 bp indel mutation in gene is associated with growth traits in Luxi Blackhead sheep.

Anim Biotechnol 2020 Apr 15:1-8. Epub 2020 Apr 15.

Shandong Key Lab of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan, Shandong, China.

Prolactin is a highly versatile pituitary hormone with multiple biological functions. expression is regulated by and the prophet of (). The aim of this study was to investigate the indel variations in ovine and the directly related ( and ) genes, and their associations with growth traits in Luxi Blackhead (LXBH) sheep. Read More

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http://dx.doi.org/10.1080/10495398.2020.1753757DOI Listing

Mining of variables from embryo morphokinetics, blastocyst's morphology and patient parameters: an approach to predict the live birth in the assisted reproduction service.

JBRA Assist Reprod 2020 Apr 15. Epub 2020 Apr 15.

Laboratório de Micromanipulação Embrionária, Department of Biological Sciences, School of Sciences and Languages, Campus Assis, São Paulo State University (UNESP), Assis, SP, Brazil.

Based on growing demand for assisted reproduction technology, improved predictive models are required to optimize in vitro fertilization/intracytoplasmatic sperm injection strategies, prioritizing single embryo transfer. There are still several obstacles to overcome for the purpose of improving assisted reproductive success, such as intra- and inter-observer subjectivity in embryonic selection, high occurrence of multiple pregnancies, maternal and neonatal complications. Here, we compare studies that used several variables that impact the success of assisted reproduction, such as blastocyst morphology and morphokinetic aspects of embryo development as well as characteristics of the patients submitted to assisted reproduction, in order to predict embryo quality, implantation or live birth. Read More

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http://dx.doi.org/10.5935/1518-0557.20200014DOI Listing