202,602 results match your criteria Assembly Automation[Journal]

Homogeneous triple-phase interfaces enabling one-pot route to metal compound/carbon composites.

J Colloid Interface Sci 2021 Apr 16;599:271-279. Epub 2021 Apr 16.

Key Laboratory for Biobased Materials and Energy of Ministry of Education, Guangdong Provincial Engineering Technology Research Center for Optical Agriculture, College of Materials and Energy, South China Agricultural University, Guangzhou, China; Guangdong Laboratory of Lingnan Modern Agriculture, Guangzhou, China. Electronic address:

Metal compounds (e.g., metal phosphides/sulfides/selenides) coupled with carbon materials have recently drawn great attraction for boosting the electrochemical performances because of their appealing synergistic effect and valuable structural stability. Read More

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Template-Induced {Mn}-Organic Framework with Lewis Acid-Base Canals as a Highly Efficient Heterogeneous Catalyst for Chemical Fixation of CO and Knoevenagel Condensation.

Inorg Chem 2021 May 4. Epub 2021 May 4.

Department of Chemistry, College of Science, North University of China, Taiyuan 030051, People's Republic of China.

The target for the self-assembly of functional microporous metal-organic frameworks (MOFs) could be realized by employing ligand-directed and/or template-induced strategies, which prompted us to explore the synthetic technique of d secondary-building-unit-based nanoporous frameworks. Here, the exquisite combination of a paddle-wheel [Mn(CO)(OH)] cluster and a TDP ligand contributes one robust honeycomb framework of {(MeNH)[Mn(TDP)(HO)]·3HO·3DMF} (; DMF = ,-dimethylformamide), whose activated state with the removal of associated aqueous molecules characterizes the outstanding physicochemical properties of nanochannels, penta- and tetracoordinated Mn serving as highly open metal sites, rich Lewis base sites (rows of C═O groups and N atoms), and excellent thermal stability. Moreover, it is worth mentioning that Lewis acid-base sites on the inner surface of the channels in activated successfully form one unprecedented canal-shaped acid-base confined space with evenly distributed open metal sites of Mn and N atoms as the canal bottom as well as two rows of C═O groups serving as dyke dams. Read More

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Fabrication of Robust Capsules by Sequential Assembly of Polyelectrolytes onto Charged Liposomes.

Langmuir 2021 May 4. Epub 2021 May 4.

Departamento de Química Física, Facultad de Ciencias Químicas, Universidad Complutense de Madrid, Ciudad Universitaria s/n, Madrid 28040, Spain.

This work presents a simple methodology for coating small unilamellar liposomes bearing different degrees of positive charge with polyelectrolyte multilayers using the sequential layer-by-layer deposition method. The liposomes were made of mixtures of 1,2-dioleyl--glycero-3-phosphocoline and dimethyl dioctadecyl ammonium bromide (DODAB) and coated by alternated layers of the sodium salt of poly(4-styrenesulfonate) (PSS) and poly(allylamine) (PAH) as polyanions and polycations, respectively. The results show that the zeta potential of the liposomes was not very sensitive to the mole fraction of DODAB in the membrane, , in the range 0. Read More

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scRNA Transcription Profile of Adult Zebrafish Podocytes Using a Novel Reporter Strain.

Cell Physiol Biochem 2021 May;55(S4):35-47

Queen's Medical Research Institute, Cardiovascular Science Centre, University of Edinburgh, Edinburgh, UK.

Background/aims: The role of podocytes is well conserved across species from drosophila to teleosts, and mammals. Identifying the molecular markers that actively maintain the integrity of the podocyte will enable a greater understanding of the changes that lead to damage.

Methods: We generated transgenic zebrafish, expressing fluorescent reporters driven by the podocin promoter, for the visualization and isolation of podocytes. Read More

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Chromosomal-level reference genome of the neotropical tree Jacaranda mimosifolia D. Don.

Genome Biol Evol 2021 May 3. Epub 2021 May 3.

Institute for Advanced Study, Chengdu University, No. 2025 Chengluo Road, Chengdu 610106, China.

Jacaranda mimosifolia D. Don is a deciduous tree widely cultivated in the tropics and subtropics of the world. It is famous for its beautiful blue flowers and pinnate compound leaves. Read More

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Characterization of the Complete Chloroplast Genome of Buddleja Lindleyana.

J AOAC Int 2021 May 4. Epub 2021 May 4.

National Engineering Research Center for Modernization of Traditional Chinese Medicine, Mathematical Engineering Academy of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, 510006, China.

Background: Buddleja lindleyana Fort., which belongs to the Loganiaceae with a distribution throughout the tropics, is widely used as an ornamental plant in China. Buddleja contains several morphologically similar species, which need to be identified by molecular identification. Read More

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Elephant Genomes Reveal Accelerated Evolution in Mechanisms Underlying Disease Defenses.

Mol Biol Evol 2021 May 3. Epub 2021 May 3.

Arizona Cancer Evolution Center, Arizona State University, Tempe, AZ.

Disease susceptibility and resistance are important factors for the conservation of endangered species, including elephants. We analyzed pathology data from 26 zoos and report that Asian elephants have increased neoplasia and malignancy prevalence compared with African bush elephants. This is consistent with observed higher susceptibility to tuberculosis and elephant endotheliotropic herpesvirus (EEHV) in Asian elephants. Read More

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Giantin is required for intracellular N-terminal processing of type I procollagen.

J Cell Biol 2021 Jun 4;220(6). Epub 2021 May 4.

Cell Biology Laboratories, School of Biochemistry, Faculty of Life Sciences, University of Bristol, Bristol, UK.

Knockout of the golgin giantin leads to skeletal and craniofacial defects driven by poorly studied changes in glycosylation and extracellular matrix deposition. Here, we sought to determine how giantin impacts the production of healthy bone tissue by focusing on the main protein component of the osteoid, type I collagen. Giantin mutant zebrafish accumulate multiple spontaneous fractures in their caudal fin, suggesting their bones may be more brittle. Read More

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Sec17/Sec18 can support membrane fusion without help from completion of SNARE zippering.

Elife 2021 May 4;10. Epub 2021 May 4.

Department of Biochemistry and Cell Biology, Geisel School of Medicine at Dartmouth, Hanover, United States.

Membrane fusion requires R-, Qa-, Qb-, and Qc-family SNAREs that zipper into RQaQbQc coiled coils, driven by the sequestration of apolar amino acids. Zippering has been thought to provide all the force driving fusion. Sec17/aSNAP can form an oligomeric assembly with SNAREs with the Sec17 C-terminus bound to Sec18/NSF, the central region bound to SNAREs, and a crucial apolar loop near the N-terminus poised to insert into membranes. Read More

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Self-Assembly of Nanosheet-Supported Fe-MOF Heterocrystals as a Reusable Catalyst for Boosting Advanced Oxidation Performance via Radical and Nonradical Pathways.

ACS Appl Mater Interfaces 2021 May 4. Epub 2021 May 4.

Department of Chemistry, Tokyo Metropolitan University, Tokyo 192-0397, Japan.

Heterojunction catalysts have drawn increasing interest for the visible light-driven Fenton reaction and bring tremendous opportunities for environmental remediation. Herein, a BiOI/MIL-53(Fe) Z-scheme heterojunction (named BMFe) was synthesized for the first time via a facile strategy. Compared with pristine BiOI and MIL-53(Fe) catalysts, the two-dimensional/three-dimensional (2D/3D) heterojunction catalyst manifested remarkable catalytic performance toward degradation of phenol, bisphenol A, methylene blue, and carbamazepine, which is attributed mainly to the interfacial integration and efficient charge separation. Read More

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Polymer Self-Assembly Induced Enhancement of Ice Recrystallization Inhibition.

J Am Chem Soc 2021 May 4. Epub 2021 May 4.

Department of Chemistry, University of Warwick, Gibbet Hill Road, CV4 7AL Coventry, U.K.

Ice binding proteins modulate ice nucleation/growth and have huge (bio)technological potential. There are few synthetic materials that reproduce their function, and rational design is challenging due to the outstanding questions about the mechanisms of ice binding, including whether ice binding is essential to reproduce all their macroscopic properties. Here we report that nanoparticles obtained by polymerization-induced self-assembly (PISA) inhibit ice recrystallization (IRI) despite their constituent polymers having no apparent activity. Read More

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Multiplexed mRNA assembly into ribonucleoprotein particles plays an operon-like role in the control of yeast cell physiology.

Elife 2021 May 4;10. Epub 2021 May 4.

Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel.

Prokaryotes utilize polycistronic messages (operons) to co-translate proteins involved in the same biological processes. Whether eukaryotes achieve similar regulation by selectively assembling and translating monocistronic messages derived from different chromosomes is unknown. We employed transcript-specific RNA pulldowns and RNA-seq/RT-PCR to identify yeast mRNAs that co-precipitate as ribonucleoprotein (RNP) complexes. Read More

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Qki regulates myelinogenesis through Srebp2-dependent cholesterol biosynthesis.

Elife 2021 May 4;10. Epub 2021 May 4.

Department of Cancer Biology, MD Anderson Cancer Center, Houston, United States.

Myelination depends on timely, precise control of oligodendrocyte differentiation and myelinogenesis. Cholesterol is the most abundant component of myelin and essential for myelin membrane assembly in the central nervous system. However, the underlying mechanisms of precise control of cholesterol biosynthesis in oligodendrocytes remain elusive. Read More

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Microscopic Imaging Techniques for Molecular Assemblies: Electron, Atomic Force, and Confocal Microscopies.

Chem Rev 2021 May 4. Epub 2021 May 4.

Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto 615-8510, Japan.

Self-assembly is promising for construction of a wide variety of supramolecular assemblies, whose 1D/2D/3D structures are typically relevant to their functions. In-depth understanding of their structure-function relationships is essential for rational design and development of functional molecular assemblies. Microscopic imaging has been used as a powerful tool to elucidate structures of individual molecular assemblies with subnanometer to millimeter resolution, which is complementary to conventional spectroscopic techniques that provide the ensemble structural information. Read More

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Condensed Clustered Iron Oxides for Ultrahigh Photothermal Conversion and Multimodal Imaging.

ACS Appl Mater Interfaces 2021 May 4. Epub 2021 May 4.

Nanotechnology Centre, Centre of Energy and Environmental Technologies, VŠB-Technical University of Ostrava, 17. listopadu 2172/15, Poruba, 708 00 Ostrava, Czech Republic.

Magnetic iron oxide nanocrystals (MIONs) are established as potent theranostic nanoplatforms due to their biocompatibility and the multifunctionality of their spin-active atomic framework. Recent insights have also unveiled their attractive near-infrared photothermal properties, which are, however, limited by their low near-infrared absorbance, resulting in noncompetitive photothermal conversion efficiencies (PCEs). Herein, we report on the dramatically improved photothermal conversion of condensed clustered MIONs, reaching an ultrahigh PCE of 71% at 808 nm, surpassing the so-far MION-based photothermal agents and even benchmark near-infrared photothermal nanomaterials. Read More

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Arylethynyl Helices Supported by π-Stacking and Halogen Bonding.

Chempluschem 2021 May;86(5):745-749

Chemistry Department, Missouri State University, 901 South National Avenue, Springfield, MO 65897, USA.

Co-crystallization of a pyridyl-containing arylethynyl (AE) moiety with 1,4-diiodotetrafluorobenzene leads to unique, figure-eight shaped helical motifs within the crystal lattice. A slight twist in the AE backbone allows each AE unit to simultaneously interact with haloarene units that are stacked on top of one another. Left-handed (M) and right-handed (P) helices are interspersed in a regular pattern throughout the crystal. Read More

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Exploring the vertebrate fauna of the Bird's Head Peninsula (Indonesia, West Papua) through DNA barcodes.

Mol Ecol Resour 2021 May 3. Epub 2021 May 3.

USR LEEISA- Laboratoire Ecologie, Interactions des Systèmes amazoniens, Centre de Recherche de Montabo, Evolution, BP 70620, 97334, French Guiana.

Biodiversity knowledge is widely heterogeneous across the Earth's biomes. Some areas, due to their remoteness and difficult access, present large taxonomic knowledge gaps. Mostly located in the tropics, these areas have frequently experienced a fast development of anthropogenic activities during the last decades and are therefore of high conservation concerns. Read More

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Strategies for Constructing and Operating DNA Origami Linear Actuators.

Small 2021 May 4:e2007704. Epub 2021 May 4.

Department of Physics, University of Oxford, Parks Road, Oxford, OX1 3PU, UK.

Linear actuators are ubiquitous components at all scales of engineering. DNA nanotechnology offers a unique opportunity for bottom-up assembly at the molecular scale, providing nanoscale precision with multiple methods for constructing and operating devices. In this paper, DNA origami linear actuators with up to 200 nm travel, based on a rail threading a topologically locked slider, are demonstrated. Read More

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Self-Assembly or Coassembly of Multiresponsive Histidine-Containing Elastin-Like Polypeptide Block Copolymers.

Macromol Biosci 2021 May 4:e2100081. Epub 2021 May 4.

Bio-Organic Chemistry, Department of Biomedical Engineering, Institute for Complex Molecular Systems, Eindhoven University of Technology, Helix (STO 3.41), P. O. Box 513, Eindhoven, 5600 MB, The Netherlands.

In this study a histidine containing elastin-like polypeptide (ELP) diblock copolymer is described with multiresponsive assembly behavior. Self-assembly into micelles is examined by two methods. First, the self-assembly is triggered by the addition of divalent metal ions, with Zn being the most suitable one. Read More

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The Pan-genome of the Cultivated Soybean (PanSoy) Reveals an Extraordinarily Conserved Gene Content.

Plant Biotechnol J 2021 May 4. Epub 2021 May 4.

Département de phytologie, Faculté des sciences de l'agriculture et de l'alimentation (FSAA), Université Laval, Québec, Québec), Canada.

Studies on structural variation in plants have revealed the inadequacy of a single reference genome for an entire species and suggest that it is necessary to build a species-representative genome, called a pan-genome to better capture the extent of both structural and nucleotide variation. Here, we present a pan-genome of cultivated soybean (Glycine max), termed PanSoy, constructed using the de novo genome assembly of 204 phylogenetically and geographically representative improved accessions selected from the larger GmHapMap collection. PanSoy uncovers 108 Mb (~11%) of novel nonreference sequences encompassing 3,621 protein-coding genes (including 1,659 novel genes) absent from the soybean "Williams 82" reference genome. Read More

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Pyroptosis in stroke-new insights into disease mechanisms and therapeutic strategies.

J Physiol Biochem 2021 May 4. Epub 2021 May 4.

State Key Laboratory of Natural Medicines, Department of Physiology, China Pharmaceutical University, Nanjing, 210009, China.

Stroke is a common disease with high mortality and disability worldwide. Different forms of cell deaths, including apoptosis and necrosis, occur in ischemic or hemorrhagic brain tissue, among which pyroptosis, a newly discovered inflammation-related programmed cell death, is generally divided into two main pathways, the canonical inflammasome pathway and the non-canonical inflammasome pathway. Caspase-mediated pyroptosis requires the assembly of inflammasomes such as NLRP3, which leads to the release of inflammatory cytokines IL-1β and IL-18 through the pores formed in the plasma membrane by GSDMD followed by neuroinflammation. Read More

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NCTR fusion facilitates the formation of TRAIL polymers that selectively activate TRAIL receptors with higher potency and efficacy than TRAIL.

Cancer Chemother Pharmacol 2021 May 4. Epub 2021 May 4.

Beijing Key Laboratory of New Drug Mechanisms and Pharmacological Evaluation Study, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, 1st, Xiannongtan Street, Xicheng District, Beijing, 100050, People's Republic of China.

Purpose: Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) binds to death receptor (DR) 4 and DR5 and induces tumor-selective apoptosis. The fusion proteins NCTR-TRAIL and NCTR-TGF3L-TRAIL self-assembled into polymers and triggered super-active cancer cell killing. The role of TGF3L in self-assembly and super-activity was unclear. Read More

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News from Mars: Two-Tier Paradox, Intracellular PCR, Chimeric Junction Shift, Dark Matter mRNA and Other Remarkable Features of Mammalian RNA-Dependent mRNA Amplification. Implications for Alzheimer's Disease, RNA-Based Vaccines and mRNA Therapeutics.

Ann Integr Mol Med 2021 ;2:131-173

Division of Molecular Medicine, Children's Hospital, USA.

Molecular Biology, a branch of science established to examine the flow of information from "letters" encrypted into DNA structure to functional proteins, was initially defined by a concept of DNA-to-RNA-to-Protein information movement, a notion termed the Central Dogma of Molecular Biology. RNA-dependent mRNA amplification, a novel mode of eukaryotic protein-encoding RNA-to-RNA-to-Protein genomic information transfer, constitutes the extension of the Central Dogma in the context of mammalian cells. It was shown to occur in cellular circumstances requiring exceptionally high levels of production of specific polypeptides, e. Read More

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January 2021

Solid-state rigid-rod polymer composite electrolytes with nanocrystalline lithium ion pathways.

Nat Mater 2021 May 3. Epub 2021 May 3.

Department of Chemistry and Macromolecules Innovation Institute, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA.

A critical challenge for next-generation lithium-based batteries lies in development of electrolytes that enable thermal safety along with the use of high-energy-density electrodes. We describe molecular ionic composite electrolytes based on an aligned liquid crystalline polymer combined with ionic liquids and concentrated Li salt. This high strength (200 MPa) and non-flammable solid electrolyte possesses outstanding Li conductivity (1 mS cm at 25 °C) and electrochemical stability (5. Read More

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Exploiting vulnerabilities of SWI/SNF chromatin remodelling complexes for cancer therapy.

Oncogene 2021 May 3. Epub 2021 May 3.

Institute of Pharmaceutical Chemistry, Goethe University Frankfurt, Frankfurt am Main, Germany.

Multi-subunit ATPase-dependent chromatin remodelling complexes SWI/SNF (switch/sucrose non-fermentable) are fundamental epigenetic regulators of gene transcription. Functional genomic studies revealed a remarkable mutation prevalence of SWI/SNF-encoding genes in 20-25% of all human cancers, frequently driving oncogenic programmes. Some SWI/SNF-mutant cancers are hypersensitive to perturbations in other SWI/SNF subunits, regulatory proteins and distinct biological pathways, often resulting in sustained anticancer effects and synthetic lethal interactions. Read More

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Biallelic splicing variants in the nucleolar 60S assembly factor RBM28 cause the ribosomopathy ANE syndrome.

Proc Natl Acad Sci U S A 2021 May;118(19)

Department of Molecular Biophysics and Biochemistry, Yale School of Medicine, New Haven, CT 06520;

Alopecia, neurologic defects, and endocrinopathy (ANE) syndrome is a rare ribosomopathy known to be caused by a p.(Leu351Pro) variant in the essential, conserved, nucleolar large ribosomal subunit (60S) assembly factor RBM28. We report the second family of ANE syndrome to date and a female pediatric ANE syndrome patient. Read More

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Archaeal and bacterial communities assembly and co-occurrence networks in subtropical mangrove sediments under Spartina alterniflora invasion.

Environ Microbiome 2021 May 3;16(1):10. Epub 2021 May 3.

College of Environmental Sciences and Engineering, Peking University, Beijing, 100871, China.

Background: Mangrove ecosystems are vulnerable due to the exotic Spartina alterniflora (S. alterniflora) invasion in China. However, little is known about mangrove sediment microbial community assembly processes and interactions under S. Read More

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One-pot synthesis of AuPd@FeO nanoagent with the activable Fe species for enhanced Chemodynamic-photothermal synergetic therapy.

Biomaterials 2021 Apr 27;274:120821. Epub 2021 Apr 27.

State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, PR China; School of Applied Chemical Engineering, University of Science and Technology of China, Road Baohe District, Hefei, Anhui, 230026, PR China. Electronic address:

Facile fabrication of Fe-based nanotheranostic agents with the enhanced Chemodynamic therapy (CDT) effect and multiple functions is important for oncotherapy. In this report, noble-metal@FeO core-shell nanoparticles (Au@FeO NPs, AuRu@FeO NPs, AuPt@FeO NPs and AuPd@FeO NPs) are one-pot constructed by a simply redox self-assembly strategy. As a typical example, AuPd@FeO NPs are applied for oncotherapy. Read More

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Distinct patterns of abundant and rare subcommunities in paddy soil during wetting-drying cycles.

Sci Total Environ 2021 Apr 24;785:147298. Epub 2021 Apr 24.

Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, 1799 Jimei Road, Xiamen, Fujian 361021, China. Electronic address:

Wetting-drying cycles typically result in a wide range of soil moistures and redox potentials (Eh) that significantly affect the soil microbial community. Although numerous studies have addressed the effects of soil moisture on soil microbial community structure and composition, the response of active microbes to the fluctuation in soil Eh is still largely unknown; this is especially true for the ecological roles of abundant and rare taxa. To explore the dynamics of active and total microbial communities in response to wetting-drying cycles, we conducted a microcosm experiment based on three wetting-drying cycles and 16S rRNA transcript (active) and 16S rRNA gene (total) amplicon sequencing. Read More

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Super-Assembled Hierarchical CoO Nanosheets-Cu Foam Composites as Multi-Level Hosts for High-Performance Lithium Metal Anodes.

Small 2021 May 3:e2101301. Epub 2021 May 3.

Collaborative Innovation Center of Chemistry for Energy Materials (iChEM), Department of Chemistry, Laboratory of Advanced Materials, Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Fudan University, Shanghai, 200433, P. R. China.

Achieving uniform lithium (Li) deposition is the key to tackle uncontrollable dendrite growth, which hinders the application of Li metal anodes. In this study, molten Li is thermally injected into a 3D framework by growing lithiophilic CoO nanosheets on Cu foam (CF). The CoO layer grown on the CF surface physically adsorbs molten Li, which makes it possible to spontaneously wet the framework. Read More

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