188,411 results match your criteria Assembly Automation[Journal]


The reference genome of the selfing fish Kryptolebias hermaphroditus: Identification of phases I and II detoxification genes.

Comp Biochem Physiol Part D Genomics Proteomics 2020 Apr 12;35:100684. Epub 2020 Apr 12.

Department of Biological Sciences, College of Science, Sungkyunkwan University, Suwon 16419, South Korea. Electronic address:

The selfing fish Kryptolebias hermaphroditus has unique reproductive system for self-fertilization, making genetically homozygous offsprings. Here, we report on high density genetic map-based genome assembly for the K. hermaphroditus Panama line (PanRS). Read More

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http://dx.doi.org/10.1016/j.cbd.2020.100684DOI Listing
April 2020
2.055 Impact Factor

Factors affecting early-life intestinal microbiota development.

Nutrition 2020 Mar 25;78:110812. Epub 2020 Mar 25.

Ulm University Hospital, Department of Pediatrics and Adolescent Medicine, Division of Pediatric Endocrinology and Diabetes, Centre for Hormonal Disorders in Children and Adolescents, Ulm, Germany.

Objectives: This paper reviews the published evidence on early-life intestinal microbiota development, as well as the different factors influencing its development before, at, and afterbirth. A literature search was done using PubMed, Cochrane and EMBASE databases. A growing body of evidence indicates that the intrauterine environment is not sterile as once presumed, but that maternal-fetal transmission of microbiota occurs during pregnancy. Read More

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http://dx.doi.org/10.1016/j.nut.2020.110812DOI Listing

Importance of exopolysaccharide branched chains in determining the aggregation ability of anammox sludge.

Sci Total Environ 2020 May 16;734:139470. Epub 2020 May 16.

National Base of International Science and Technology Cooperation for Intelligent Manufacturing Service, Chongqing Key Laboratory of Catalysis & New Environmental Materials, Chongqing Technology and Business University, Chongqing 400067, China; Chongqing South-to-Thais Environmental Protection Technology Research Institute Co., Ltd., Chongqing 400060, China. Electronic address:

The high aggregation ability of anammox granular sludge is an issue of wide concern; however, the mechanism needs to be further clarified. In this study, selective hydrolysis experiments were performed to determine the role of exopolysaccharide (PS) branched chains and proteins for the aggregation mechanism of anammox granular sludge. The results revealed that selective hydrolysis of proteins hardly affected the granular aggregation while the hydrolysis of PS branched chains led to a decrease in the sludge zeta potential by 17. Read More

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http://dx.doi.org/10.1016/j.scitotenv.2020.139470DOI Listing

Sequence-Independent Self-Assembly of Germ Granule mRNAs into Homotypic Clusters.

Mol Cell 2020 May 20. Epub 2020 May 20.

HHMI, Skirball Institute of Biomolecular Medicine, Department of Cell Biology, NYU School of Medicine, New York, NY, USA. Electronic address:

mRNAs enriched in membraneless condensates provide functional compartmentalization within cells. The mechanisms that recruit transcripts to condensates are under intense study; however, how mRNAs organize once they reach a granule remains poorly understood. Here, we report on a self-sorting mechanism by which multiple mRNAs derived from the same gene assemble into discrete homotypic clusters. Read More

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http://dx.doi.org/10.1016/j.molcel.2020.05.008DOI Listing

A Reversible Light-Responsive Solventless-Liquid Switch: Polarization Induced Dynamic Surface Ordering-Disordering in Liquid-Like Carbon Quantum Dots.

J Phys Chem Lett 2020 May 28. Epub 2020 May 28.

Naturally stimulated dynamic ordering-disordering of biomolecules via non-covalent interactions is a commonly ocuring phenomenon in biological systems. Herein, we report the effect of induced polarization on the charge carrier dynamicsof carbon quantum dot-based nano ionic materials (CQD-NIMs) under simulated solar radiation. The solvent-less liquid-like CQD-NIMs is composed of polystyrene sulfonate (PSS)-passivated CQD as core-coronasystem with a polyetheramine (Jeffamine®) forming the canopy. Read More

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http://dx.doi.org/10.1021/acs.jpclett.0c01275DOI Listing

Cyanide-Bridged Fe-Co Polynuclear Clusters Based on Four-Coordinate Cobalt(II).

Inorg Chem 2020 May 28. Epub 2020 May 28.

Department of Chemistry, Southern University of Science and Technology (SUSTech), Shenzhen, 518055, China.

Treatment of CoCl·6HO and tris(pyrazolyl-1-yl)borate tricyanoiron(III) anions at 55 °C afforded a series of new Fe-Co polynuclear clusters: {CoCl(DMF)[(Tp)Fe(CN)]} (; Tp = hydridotris(4-methylpyrazol-1-yl)borate), (HO)@{CoCl[(Tp)Fe(CN)]} (), (MePhP){CoCl[(Tp)Fe(CN)]}·15CHCN·3CHOH·2HO (), and (BnEtN){CoCl[(Tp*)Fe(CN)]}·4CHCN·2HO (; Tp*= hydridotris(3,5-dimethylpyrazol-1-yl)borate). They feature an asymmetric [FeCo(CN)] square, a pseudocubic [FeCo(CN)] cluster, a distorted-hexagonal-prism-shaped [FeCo(CN)] cage, and a bis(trigonal-bipyramidal) cluster of [FeCo(CN)] fused at one cobalt center, respectively. The Co(II) ions adopt a four-coordinate tetrahedral geometry except for half of in an octahedral geometry. Read More

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http://dx.doi.org/10.1021/acs.inorgchem.0c00233DOI Listing

Autonomous growth of spatially localized supramolecular hydrogel through emergence of autocatalytic ability.

Angew Chem Int Ed Engl 2020 May 28. Epub 2020 May 28.

Institut Charles Sadron (UPR22 - CNRS), PECMAT, 23 rue du Loess, 67034, STRASBOURG, FRANCE.

Autocatalysis and self-assembly are key processes in developmental biology and are involved in the emergence of life. In the last decade both features were extensively investigated by chemists with the final goal to design synthetic living systems. Herein, we describe the autonomous growth of a self-assembled soft material, i. Read More

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http://dx.doi.org/10.1002/anie.202005377DOI Listing

Freedom of assembly: metabolic enzymes come together.

Mol Biol Cell 2020 Jun;31(12):1201-1205

Cancer Biology Program, Fox Chase Cancer Center, Philadelphia, PA 19111.

Many different enzymes in intermediate metabolism dynamically assemble filamentous polymers in cells, often in response to changes in physiological conditions. Most of the enzyme filaments known to date have only been observed in cells, but in a handful of cases structural and biochemical studies have revealed the mechanisms and consequences of assembly. In general, enzyme polymerization functions as a mechanism to allosterically tune enzyme kinetics, and it may play a physiological role in integrating metabolic signaling. Read More

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http://dx.doi.org/10.1091/mbc.E18-10-0675DOI Listing

Quantitative Analysis of Nanorod Aggregation and Morphology from Scanning Electron Micrographs Using SEMseg.

J Phys Chem A 2020 May 28. Epub 2020 May 28.

General methods to achieve better physical insight about nanoparticle aggregation and assembly are needed because of the potential role of aggregation in a wide range of materials, environmental, and biological outcomes. Scanning electron microscopy (SEM) is fast and affordable compared to transmission electron microscopy, but SEM micrographs lack contrast and resolution due to lower beam energy, topographic contrast, edge effects, and charging. We present a new segmentation algorithm called SEMseg that is robust to the challenges inherent in SEM micrograph analysis and demonstrate its utility for analyzing gold (Au) nanorod aggregates. Read More

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http://dx.doi.org/10.1021/acs.jpca.0c03190DOI Listing

Supramolecular Co-assembly of the TiL Cube with [Ti(DMF)] Species and Ti-Oxo Cluster.

Inorg Chem 2020 May 28. Epub 2020 May 28.

State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002, P. R. China.

Titanium-based coordination cages are fascinating in the field of supramolecular and photophysical chemistry. Herein, we address the unprecedented supramolecular co-assembly arrangement of a cubic TiL cage with [Ti(DMF)] species and Ti-oxo cluster, contributing to the cocrystals of {TiL + Ti(DMF)} () and {TiL + Ti-oxo} (). The ESI-MS and H NMR measurements reveal their stability in solution. Read More

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http://dx.doi.org/10.1021/acs.inorgchem.0c00682DOI Listing

Efficient water self-diffusion in diphenylalanine peptide nanotubes.

ACS Appl Mater Interfaces 2020 May 28. Epub 2020 May 28.

Nanotubes of self-assembled dipeptides as exemplified by diphenylalanine (FF) demonstrate a wide range of useful functional properties such as high Young's modulus, strong photoluminescence, remarkable piezoelectricity and pyroelectricity, optical waveguiding, etc., and became an object of the intensive research due to their ability to combine electronic and biological functions in the same material. Two types of nanoconfined water molecules (bound water directly interacting with peptide backbone and free water located inside nanochannels) are known to play a key role in the self-assembly of FF. Read More

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http://dx.doi.org/10.1021/acsami.0c03658DOI Listing

Transfer cells: What regulates the development of their intricate wall labyrinths?

New Phytol 2020 May 28. Epub 2020 May 28.

School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW, 2308, Australia.

Transfer cells (TCs) support high nutrient rates into, or at symplasmic discontinuities within, the plant body. Their transport capacity is conferred by an amplified plasma membrane surface area, enriched in nutrient transporters, supported on an intricately-invaginated wall labyrinth (WL). Thus, development of the WL is at the heart of TC function. Read More

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http://dx.doi.org/10.1111/nph.16707DOI Listing

DNA-Programmed plasmonic ELISA for the ultrasensitive detection of protein biomarkers.

Analyst 2020 May 28. Epub 2020 May 28.

State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, P. R. China.

We report a novel DNA-programmed plasmonic enzyme-linked immunosorbent assay (ELISA) for the ultrasensitive detection of protein biomarkers with the naked eye. The DNA-programmed plasmonic assay was based on two enzyme-free and isothermal nucleic acid amplification methods: hybridization chain reaction (HCR) and catalyzed hairpin assembly (CHA). In this study, a biotin-labeled DNA probe was utilized insteand of an enzyme-label probe in well-developed ELISA method. Read More

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http://dx.doi.org/10.1039/d0an00656dDOI Listing

Zeta-Potential Dependent Self-Assembly For Very Large Area Nanosphere Lithography.

Nano Lett 2020 May 28. Epub 2020 May 28.

Nanosphere lithography offers a rapid, low-cost approach for patterning of large-area two dimensional periodic nanostructures. However, a complete understanding of the nanosphere self-assembly process is necessary to enable further development and scaling of this technology. The self-assembly of nanospheres into two dimensional periodic arrays has previously been attributed solely to the Marangoni force; however, we demonstrate that the zeta potential of the nanosphere solution is critically important for successful self-assembly to occur. Read More

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http://dx.doi.org/10.1021/acs.nanolett.0c01277DOI Listing

Molecular-Pump-Enabled Synthesis of a Daisy Chain Polymer.

J Am Chem Soc 2020 May 28. Epub 2020 May 28.

Department of Chemistry, Northwestern University, 2145 Sheridan Road, Evanston, Illinois 60208, United States.

The assembly of a kinetically trapped daisy chain polymer under redox control has been achieved with a self-complementary monomer using an energy ratchet mechanism. The monomer is composed of a molecular pump at one end and a cyclobis(paraquat--phenylene) (CBPQT) ring at the other end. The pump and ring are linked together by a long collecting chain. Read More

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http://dx.doi.org/10.1021/jacs.0c04029DOI Listing

Radicalradical chalcogen bonds: CSD analysis and DFT calculations.

Phys Chem Chem Phys 2020 May 28. Epub 2020 May 28.

Laboratory of Nanostructured Fluorinated Materials (NFMLab), Department of Chemistry, Materials and Chemical Engineering "Giulio Natta", Politecnico di Milano, Via L. Mancinelli 7, 20131 Milano, Italy.

This manuscript reports a combination of crystallographic analysis (Cambridge Structural Database) and theoretical DFT calculations in chalcogen bonding interactions involving radicals in both the Ch bond (ChB) donor and acceptor. As a radical ChB acceptor (nucleophile) we have used benzodithiazolyl radical (BDTA) and as Ch bond donors (electrophile) we have used dithiadiazolyl and diselenadiazolyl radicals of the general formula p-X-C6F4-CNChChN (Ch = S, and Se). We have evaluated how the para substituent (X) affects the interaction energy, spin density and charge/spin transfer from the electron rich BDTA radical to the electron poor dichalcogenadiazolyl ring. Read More

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http://dx.doi.org/10.1039/d0cp01643hDOI Listing

A computational prediction of SARS-CoV-2 structural protein inhibitors from (Neem).

J Biomol Struct Dyn 2020 May 28:1-17. Epub 2020 May 28.

Kusuma School of Biological Sciences, Indian Institute of Technology Delhi, Hauz Khas, New Delhi, 110016, India.

The rapid global spread of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has created an unprecedented healthcare crisis. The treatment for the severe respiratory illness caused by this virus is primarily symptomatic at this point, although the usage of a broad antiviral drug Remdesivir has been allowed on emergency basis by the Food and Drug Administration (FDA).The ever-increasing death toll highlights an urgent need for development of specific antivirals. Read More

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http://dx.doi.org/10.1080/07391102.2020.1774419DOI Listing

2D Supramolecular-Ionic Framework for Precise Membrane Separation of Small Nanoparticles.

ACS Appl Mater Interfaces 2020 May 28. Epub 2020 May 28.

Supramolecular frameworks driven by intermolecular interactions represent a new type of porous materials differing from those driven by covalent or coordination bonding. The intermolecular interaction-induced flexible assembly structures display unique advantages in materials processing, structure stimuli-responses and recyclings. In this work, a two-dimensional (2D) supramolecular-ionic framework (SIF) was constructed through the initial ionic interaction between host cation and polyoxometalate polyanion and then the host-guest inclusion of the formed host ionic complex with a four-arm porphyrin guest molecule following a 2+4 reaction. Read More

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http://dx.doi.org/10.1021/acsami.0c05947DOI Listing

Self-Assembly of Hydrophobic and Self-Healing Bio-nanocomposite-Coated Controlled Release Fertilizer.

ACS Appl Mater Interfaces 2020 May 28. Epub 2020 May 28.

Self-healing materials have received increased attentions due to their automatic detecting and repairing damage function. In this paper, a novel self-assembly and self-healing bio-nanocomposite was developed as a coating material for controlled release fertilizer. This nanotechnology-enabled coating is environmentally friendly, highly efficient, and possesses tunable nutrient-releasing characteristic. Read More

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http://dx.doi.org/10.1021/acsami.0c06530DOI Listing

Mutually constructive roles of Ail and LPS in Yersinia pestis serum survival.

Mol Microbiol 2020 May 27. Epub 2020 May 27.

Cancer Center, Sanford Burnham Prebys Medical Discovery Institute, 10901 North Torrey Pines Road, La Jolla, CA, 92037, USA.

The outer membrane is a key virulence determinant of gram-negative bacteria. In Yersinia pestis, the deadly agent that causes plague, the protein Ail and lipopolysaccharide (LPS) enhance lethality by promoting resistance to human innate immunity and antibiotics, enabling bacteria to proliferate in the human host. Their functions are highly coordinated. Read More

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http://dx.doi.org/10.1111/mmi.14530DOI Listing

Automated Optical-Tweezers Assembly of Engineered Microgranular Crystals.

Small 2020 May 27:e2000314. Epub 2020 May 27.

Department of Mechanical and Aerospace Engineering, University of California, Los Angeles, Los Angeles, CA, 90095, USA.

In this work, a scalable automated approach for fabricating 3D microgranular crystals consisting of desired arrangements of microspheres using holographic optical tweezers and two-photon polymerization is introduced. The ability to position microspheres as desired within lattices of any configuration allows designers to engineer the behavior of new metamaterials that enable advanced applications (e.g. Read More

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http://dx.doi.org/10.1002/smll.202000314DOI Listing

Assembly of Foldable 3D Microstructures Using Graphene Hinges.

Adv Mater 2020 May 27:e2001303. Epub 2020 May 27.

School of Electrical and Electronic Engineering, Yonsei University, Seoul, 03722, Republic of Korea.

Origami/kirigami-inspired 3D assembly approaches have recently attracted attention for a variety of applications, such as advanced optoelectronic devices and biomedical sensors. The results reported here describe an approach to construct classes of multiple foldable 3D microstructures that involve deformations that typical conductive materials, such as conventional metal films, cannot tolerate. Atomically thin graphene sheets serve as folding hinges during a process of 2D to 3D conversion via a deterministic buckling process. Read More

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http://dx.doi.org/10.1002/adma.202001303DOI Listing

Methods for Optimization of Protein Extraction and Proteogenomic Mapping in Sweet Potato.

Methods Mol Biol 2020 ;2139:309-324

Department of Biochemistry, Molecular Biology, Entomology, and Plant Pathology, Mississippi State University, Mississippi State, MS, USA.

The complexity in chemical composition alongside the genomic complexity of crop plants poses significant challenges for the characterization of their proteomes. This chapter provides specific methods that can be used for the extraction and identification of proteins from sweet potato, and a proteogenomic method for the subsequent peptide mapping on the haplotype-derived sweet potato genome assembly. We outline two basic methods for extracting proteins expressed in root and leaf tissues for the label-free quantitative proteomics-one phenol-based procedure and one polyethylene glycol (PEG) 4000-based fractionation method-and discuss strategies for the organ-specific protein extraction and increased recovery of low-abundance proteins. Read More

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http://dx.doi.org/10.1007/978-1-0716-0528-8_23DOI Listing
January 2020

Self-assembly of novel Fmoc-cardanol compounds into hydrogels - analysis based on rheological, structural and thermal properties.

Soft Matter 2020 May 28. Epub 2020 May 28.

Polymer Science and Engineering Division, CSIR-National Chemical Laboratory, Dr Homibhabha Road, Pune, 411008, India.

Hydrogels of low molecular weight molecules are particularly appealing for various biomedical applications such as drug delivery, tissue engineering, and antitumor therapy due to their excellent biocompatibility, biodegradability, and easy availability. Fmoc-peptide hydrogels form an essential category of these hydrogels. Herein we report a new class of Fmoc hydrogels in which cardanol (3-pentadecyl phenol (PDP)) is covalently linked with fluorenylmethyloxycarbonyl group. Read More

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http://dx.doi.org/10.1039/d0sm00670jDOI Listing

Controlled supramolecular polymerization of π-systems.

Chem Commun (Camb) 2020 May 28. Epub 2020 May 28.

School of Applied and Interdisciplinary Sciences, Indian Association for the Cultivation Science, 2A and 2B Raja S. C. Mullick Road, Kolkata, 700032, India.

Supramolecular polymers, albeit having precise internal order, largely lack precision in the mesoscopic scale because in most examples supramolecular polymerization occurs under thermodynamic control through spontaneous self-assembly. Recent reports have exemplified that by varying experimental parameters including cooling rate, solvent composition, interplay of intra- vs. inter-molecular H-bonding and others, it is possible to retard the spontaneous nucleation, and isolate a dormant kinetically controlled monomeric/aggregated state which in turn can serve as the monomer pool to undergo controlled supramolecular polymerization (CSP) through a chain-growth mechanism in the presence of an aggregated/molecular initiator (seed) or by an external stimuli like light. Read More

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http://dx.doi.org/10.1039/d0cc02787aDOI Listing

Drivers of beta diversity in modern and ancient reef-associated soft-bottom environments.

PeerJ 2020 14;8:e9139. Epub 2020 May 14.

GeoZentrum Nordbayern, Section Paleobiology, Friedrich-Alexander University Erlangen-Nürnberg, Erlangen, Germany.

Beta diversity, the compositional variation among communities, is often associated with environmental gradients. Other drivers of beta diversity include stochastic processes, priority effects, predation, or competitive exclusion. Temporal turnover may also explain differences in faunal composition between fossil assemblages. Read More

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http://dx.doi.org/10.7717/peerj.9139DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7231500PMC

The stepwise assembly of the neonatal virome is modulated by breastfeeding.

Nature 2020 May 15;581(7809):470-474. Epub 2020 Apr 15.

Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.

The gut of healthy human neonates is usually devoid of viruses at birth, but quickly becomes colonized, which-in some cases-leads to gastrointestinal disorders. Here we show that the assembly of the viral community in neonates takes place in distinct steps. Fluorescent staining of virus-like particles purified from infant meconium or early stool samples shows few or no particles, but by one month of life particle numbers increase to 10 per gram, and these numbers seem to persist throughout life. Read More

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http://dx.doi.org/10.1038/s41586-020-2192-1DOI Listing

Long-read bitter gourd () genome and the genomic architecture of nonclassic domestication.

Proc Natl Acad Sci U S A 2020 May 27. Epub 2020 May 27.

Institute of Ecology and Evolutionary Biology, National Taiwan University, Taipei 10617, Taiwan;

The genetic architecture of quantitative traits is determined by both Mendelian and polygenic factors, yet classic examples of plant domestication focused on selective sweep of newly mutated Mendelian genes. Here we report the chromosome-level genome assembly and the genomic investigation of a nonclassic domestication example, bitter gourd (), an important Asian vegetable and medicinal plant of the family Cucurbitaceae. Population resequencing revealed the divergence between wild and South Asian cultivars about 6,000 y ago, followed by the separation of the Southeast Asian cultivars about 800 y ago, with the latter exhibiting more extreme trait divergence from wild progenitors and stronger signs of selection on fruit traits. Read More

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http://dx.doi.org/10.1073/pnas.1921016117DOI Listing

Force and phosphate release from Arp2/3 complex promote dissociation of actin filament branches.

Proc Natl Acad Sci U S A 2020 May 27. Epub 2020 May 27.

Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT 06520;

Networks of branched actin filaments formed by Arp2/3 complex generate and experience mechanical forces during essential cellular functions, including cell motility and endocytosis. External forces regulate the assembly and architecture of branched actin networks both in vitro and in cells. Considerably less is known about how mechanical forces influence the disassembly of actin filament networks, specifically, the dissociation of branches. Read More

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http://dx.doi.org/10.1073/pnas.1911183117DOI Listing

A genetically engineered rotavirus NSP2 phosphorylation mutant impaired in viroplasm formation and replication shows an early interaction between vNSP2 and cellular lipid droplets.

J Virol 2020 May 27. Epub 2020 May 27.

Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX 77030

Many RNA viruses replicate in cytoplasmic compartments (virus factories or viroplasms) composed of viral and cellular proteins, but the mechanisms required for their formation remain largely unknown. Rotavirus (RV) replication in viroplasms requires interactions between virus nonstructural proteins NSP2 and NSP5 that are associated with components of lipid droplets (LDs). We previously identified two forms of NSP2 in RV-infected cells; a cytoplasmically dispersed (dNSP2) and a viroplasm-specific (vNSP2) that interact with hypo- and hyper-phosphorylated NSP5, respectively, that indicates a coordinated phosphorylation cascade controls viroplasm assembly. Read More

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http://dx.doi.org/10.1128/JVI.00972-20DOI Listing

Solvent induced supramolecular polymorphism in Cu(II) coordination complex built from 1,2,4-triazolo[1,5-a]pyrimidine: Crystal structures and anti-oxidant activity.

J Inorg Biochem 2020 May 5:111092. Epub 2020 May 5.

Institute of Condensed Matter and Nanosciences, Molecular Chemistry, Materials and Catalysis (IMCN/MOST), Université catholique de Louvain, Place L. Pasteur 1, 1348 Louvain-la-Neuve, Belgium. Electronic address:

Two Cu(II) coordination complexes, C1 and C2 of the formula [Cu(4)(HO)], have been prepared by reaction between CuCl·2HO and 7-ethoxycarbonylmethyl-5-methyl-1,2,4[1,5-a]pyrimidine (L) in a 1:2 M:L molar ratio. The L molecule decomposes during the reaction process into 7-carboxy-5-methyl-[1,2,4]-triazolo[1,5-a]pyrimidine (4) through an intermediate, ethyl 2,2-dihydroxy-2-(5-methyl-[1,2,4]triazolo[1,5-a]pyrimidin-7-yl)acetate (5), which has been isolated and its crystal structure determined by X-ray diffraction. The X-ray analysis of the single crystals of [Cu(4)(HO)] obtained from the slow evaporation of EtOH and MeOH, separately, revealed the formation of "solvent induced" polymorphs C1 and C2, respectively. Read More

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http://dx.doi.org/10.1016/j.jinorgbio.2020.111092DOI Listing

Vascular Extracellular Matrix Remodeling and Hypertension.

Antioxid Redox Signal 2020 May 27. Epub 2020 May 27.

Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Peking University, Beijing, China.

The vascular extracellular matrix (ECM) not only provides mechanical stability but also manipulates vascular cell behaviors, which are crucial for vascular function and homeostasis. ECM remodeling, which alters vascular wall mechanical properties and exposes vascular cells to bioactive molecules, is involved in the development and progression of hypertension. This brief review summarized the dynamic changes in ECM components and their modification and degradation during hypertension and after antihypertensive treatment. Read More

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http://dx.doi.org/10.1089/ars.2020.8110DOI Listing

The Effect of Cholesterol on Membrane Binding and Self-Assembly of Collagen Fibrils.

Langmuir 2020 May 27. Epub 2020 May 27.

Collagen is a skeleton of the native extracellular matrix (ECM) that is known to provide mechanical and structural stability. In an attempt to develop a new connective cellular model with the surrounding ECM without further experimental complications, such as the reconstitution of ECM receptors, we designed the experiments and discovered that the fibrillogenesis of membrane-bound collagen is not spontaneous as it is in the form of free collagen in bulk solution. The confocal microscopic results suggest that cholesterol is a crucial component that facilitates the fibril formation on the membrane surface. Read More

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http://dx.doi.org/10.1021/acs.langmuir.0c00574DOI Listing

Layer-by-layer assembly of bio-inspired borate/graphene oxide membranes for dye removal.

Chemosphere 2020 May 19;256:127118. Epub 2020 May 19.

Nanjing Institute of Environmental Science, Ministry of Ecology and Environment of the People's Republic of China, Nanjing, 210042, China. Electronic address:

Dye wastewater is harmful to the ecological environment because of its potential biological toxicity, teratogenicity, carcinogenicity, and mutagenicity. We fabricated a layered graphene oxide (GO) membrane through layer-by-layer (LBL) self-assembly. We used borate to crosslink with GO on a polyethyleneimine (PEI)-coated hydrolyzed polyacrylonitrile (hPAN) support. Read More

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http://dx.doi.org/10.1016/j.chemosphere.2020.127118DOI Listing

Self-assembling nanoparticles of dually hydrophobic prodrugs constructed from camptothecin analogue for cancer therapy.

Eur J Med Chem 2020 Apr 30;200:112365. Epub 2020 Apr 30.

Key Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao, 266003, China; Laboratory for Marine Drugs and Bioproducts, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266237, China. Electronic address:

Nanomedicines have shown success in cancer therapy in recent years because of their excellent solubility in aqueous solution and drug accumulation through controlled release in tumor tissues, but the preparation of most nanomedicines still requires ionic materials, surfactants or the amphiphilic structure to maintain nanoparticle stability and function. In this study, we developed a couple of novel dually hydrophobic prodrugs (DHPs) by combining two hydrophobic compounds through different linkers and elaborated their self-assembly mechanisms by virtue of computational simulation. Importantly, without using any excipients, FL-2 NPs exhibited significantly prolonged retention in blood circulation and displayed a remarkable anti-tumor effect at very low concentration in vivo. Read More

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http://dx.doi.org/10.1016/j.ejmech.2020.112365DOI Listing

Direct conjugation of distinct carbon dots as Lego-like building blocks for the assembly of versatile drug nanocarriers.

J Colloid Interface Sci 2020 May 5;576:412-425. Epub 2020 May 5.

Department of Chemistry, University of Miami, Coral Gables, FL 33146, USA. Electronic address:

As a promising drug nanocarrier, carbon dots (CDs) have exhibited many excellent properties. However, some properties such as bone targeting and crossing the blood-brain barrier (BBB) only apply to a certain CD preparation with limited drug loading capacity. Therefore, it is significant to conjugate distinct CDs to centralize many unique properties on the novel drug nanocarrier. Read More

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http://dx.doi.org/10.1016/j.jcis.2020.05.005DOI Listing

Oxford Nanopore Sequencing: New opportunities for plant genomics?

J Exp Bot 2020 May 27. Epub 2020 May 27.

Institute for Biology I, BioSC, RWTH Aachen University.

DNA sequencing was dominated by Sanger's chain-termination method until the mid-2000s, when it was progressively supplanted by new sequencing technologies that can generate much larger quantities of data in a shorter time. At the forefront of these developments, long-read sequencing technologies (third-generation sequencing) can produce reads that are several kilobases in length. This greatly improves the accuracy of genome assemblies by spanning the highly-repetitive segments that cause difficulty for second-generation short-read technologies. Read More

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http://dx.doi.org/10.1093/jxb/eraa263DOI Listing

Just-in-Time Simulation to Guide Workflow Design for Coronavirus Disease 2019 Difficult Airway Management.

Pediatr Crit Care Med 2020 May 27. Epub 2020 May 27.

Department of Anesthesiology and Critical Care Medicine, The Children's Hospital of Philadelphia, The University of Pennsylvania Perlman School of Medicine, Philadelphia, PA.

Objectives: The coronavirus disease 2019 pandemic has required that hospitals rapidly adapt workflows and processes to limit disease spread and optimize the care of critically ill children.

Design And Setting: As part of our institution's coronavirus disease 2019 critical care workflow design process, we developed and conducted a number of simulation exercises, increasing in complexity, progressing to intubation wearing personal protective equipment, and culminating in activation of our difficult airway team for an airway emergency.

Patients And Interventions: In situ simulations were used to identify and rework potential failure points to generate guidance for optimal airway management in coronavirus disease 2019 suspected or positive children. Read More

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http://dx.doi.org/10.1097/PCC.0000000000002435DOI Listing

Exploring the Determinants of Mobile Health Adoption by Hospitals in China.

JMIR Med Inform 2019 Nov 22. Epub 2019 Nov 22.

Faculty of Health and Applied Sciences, University of the West of England, Bristol, GB.

Background: Although mobile health (mHealth) has the potential to transform health care by delivering better outcomes at a much lower cost than traditional health care services, little is known about mHealth adoption by hospitals.

Objective: This study aimed to explore the determinants of mHealth adoption by hospitals using the technology-organization-environment (TOE) framework.

Methods: We conducted an interviewer-administered survey with 87 managers in Chinese public hospitals and analyzed the data using logistic regression. Read More

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http://dx.doi.org/10.2196/14795DOI Listing
November 2019

Switchable Full-Color Reflective Photonic Ellipsoidal Particles.

J Am Chem Soc 2020 May 27. Epub 2020 May 27.

Department of Chemistry, Massachusetts Institute of Technology (MIT), Cambridge, Massachusetts 02139, United States.

Full-color reflective photonic ellipsoidal polymer particles, capable of a dynamic color change, are created from dendronized brush block copolymers (-BBCPs) self-assembled by solvent-evaporation from an emulsion. Surfactants composed of dendritic monomer units allow for the precise modulation of the interfacial properties of -BBCP particles to transition in shape from spheres to striped ellipsoids. Strong steric repulsions between wedge-type monomers promote rapid self-assembly of polymers into large domains (i. Read More

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http://dx.doi.org/10.1021/jacs.0c02398DOI Listing

The integrity of the intradimer interface of the Hepatitis B Virus capsid protein dimer regulates capsid self-assembly.

ACS Chem Biol 2020 May 27. Epub 2020 May 27.

During the hepatitis B virus lifecycle, 120 copies of homodimeric capsid protein assemble around a copy of reverse transcriptase and viral RNA and go on to produce an infectious virion. Assembly needs to be tightly regulated by protein conformational change to ensure symmetry, fidelity and reproducibility. Here we show that structures at the intradimer interface regulate conformational changes at the distal interdimer interface and so regulate assembly. Read More

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http://dx.doi.org/10.1021/acschembio.0c00277DOI Listing

Enhanced Reduction of Thermal Conductivity in Amorphous Silicon Nitride Containing Phononic Crystals Fabricated Using Directed Self-Assembly of Block Copolymers.

ACS Nano 2020 May 27. Epub 2020 May 27.

Studies have demonstrated that the thermal conductivity () of crystalline semiconductor materials can be reduced by phonon scattering in periodic nanostructures formed using templates fabricated from self-assembled block copolymers (BCPs). Compared to crystalline materials, the heat transport mechanisms in amorphous inorganic materials differ significantly and have been explored far less extensively. However, thermal management of amorphous inorganic solids is crucial for a broad range of semiconductor devices. Read More

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http://dx.doi.org/10.1021/acsnano.0c01463DOI Listing

Chiral Assembly of Gold-Silver Core-Shell Plasmonic Nanorods on DNA Origami with Strong Optical Activity.

ACS Nano 2020 May 27. Epub 2020 May 27.

The spatial organization of metal nanoparticles has become an important tool for manipulating light in nanophotonic applications. Silver nanoparticles, particularly silver nanorods have excellent plasmonic properties, but are prone to oxidation and are therefore inherently unstable in aqueous solutions and salt containing buffers. Consequently, gold nanoparticles have often been favored, despite their inferior optical performance. Read More

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http://dx.doi.org/10.1021/acsnano.0c03127DOI Listing

Long-Range Exciton Diffusion in Two-Dimensional Assemblies of Cesium Lead Bromide Perovskite Nanocrystals.

ACS Nano 2020 May 27. Epub 2020 May 27.

Förster Resonant Energy Transfer (FRET)-mediated exciton diffusion through artificial nanoscale building block assemblies could be used as an optoelectronic design element to transport energy. However, so far nanocrystal (NC) systems supported only diffusion lengths of 30 nm, which are too small to be useful in devices. Here, we demonstrate a FRET-mediated exciton diffusion length of 200 nm with 0. Read More

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http://dx.doi.org/10.1021/acsnano.0c01536DOI Listing

Transparent, flexible MAPbI perovskite microwire arrays passivated with ultra-hydrophobic supramolecular self-assembly for stable and high-performance photodetectors.

Nanoscale 2020 May 27. Epub 2020 May 27.

School of Applied & Interdisciplinary Sciences, Indian Association for the Cultivation of Science, Jadavpur, Kolkata 700032, India. and Technical Research Centre, Indian Association for the Cultivation of Science, Jadavpur, Kolkata 700032, India. and Department of Organic Chemistry, Indian Institute of Science, Bangalore 560012, Karnataka, India.

The emergence of organic-inorganic hybrid perovskites (OHPs) has revolutionised the potential performance of optoelectronic devices; most perovskites are opaque and hence incompatible with transparent optoelectronics and sensitive to environmental degradation. Here, we have reported a single-step fabrication of ultra-long MAPbI3 perovskite microwire arrays over a large area using stencil lithography based on sequential vacuum sublimation. The environmental stability of MAPbI3 is empowered with a newly designed and synthesized transparent supramolecular self-assembly based on a mixture of two tripodal l-Phe-C11H23/C7F15 molecules, which showed a contact angle of 105° and served as ultra-hydrophobic passivation layers for more than 45 days in an ambient atmosphere. Read More

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http://dx.doi.org/10.1039/d0nr01394cDOI Listing

Integrating CuInSe nanocrystals with polymeric carbon nitride nanorods for photocatalytic water splitting.

Dalton Trans 2020 May 27. Epub 2020 May 27.

Fujian Key Laboratory of Photoelectric Functional Materials, College of Materials Science and Engineering, Huaqiao University, Xiamen, Fujian 361021, P. R. China.

Developing photocatalysts with improved photoactivity and efficiency has remained an enduring theme both fundamentally and technologically in the field of photocatalysis. Polymeric carbon nitride (CN) has been widely exploited as an earth-abundant photocatalyst for water redox reactions. Nevertheless, the limited visible-light utilization rate and the high recombination rate of photoinduced charge carriers give rise to the moderate photocatalytic reactivity of CN in water splitting. Read More

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http://dx.doi.org/10.1039/d0dt00865fDOI Listing

Structural insights into sodium transport by the oxaloacetate decarboxylase sodium pump.

Elife 2020 May 27;9. Epub 2020 May 27.

Department of Biochemistry and Molecular Biology, Tianjin Medical University, Tianjin, China.

The oxaloacetate decarboxylase sodium pump (OAD) is a unique primary-active transporter that utilizes the free energy derived from oxaloacetate decarboxylation for sodium transport across the cell membrane. It is composed of 3 subunits: the a subunit catalyzes carboxyl-transfer from oxaloacetate to biotin, the membrane integrated β subunit catalyzes the subsequent carboxyl-biotin decarboxylation and the coupled sodium transport, the γ subunit interacts with the a and β subunits and stabilizes the OAD complex. We present here structure of the OAD βγ sub-complex. Read More

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http://dx.doi.org/10.7554/eLife.53853DOI Listing

Ultrafast Growth of Cu(OH)2-CuO Nanoneedle Array on Cu Foil for Methanol Oxidation Electrocatalysis.

ACS Appl Mater Interfaces 2020 May 27. Epub 2020 May 27.

A swift potentiostatic anodization method for growing 5-7 µm tall nanoneedle array of Cu(OH)2-CuO on Cu foil within 100 s has been developed. This catalytic electrode when screened for methanol oxidation electrocatalysis in 1 M KOH with 0.5 M methanol, delivered a current density as high as 70 ± 10 mA cm-2 at 0. Read More

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http://dx.doi.org/10.1021/acsami.0c08979DOI Listing

Guanidinylated Amphiphilic Polycarbonates with Enhanced Antimicrobial Activity by Extending the Length of the Spacer Arm and Micelle Self-Assembly.

Macromol Biosci 2020 May 27:e2000065. Epub 2020 May 27.

School of Chemical Sciences, University of Auckland, Auckland, 1142, New Zealand.

Nine guanidinylated amphiphilic polycarbonates are rationally designed and synthesized. Each polymer has the same biodegradable backbone but different side groups. The influence of the hydrophobic/hydrophilic effect on antimicrobial activities and cytotoxicity is systematically investigated. Read More

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http://dx.doi.org/10.1002/mabi.202000065DOI Listing

Unveiling the Dynamical Assembly of Magnetic Nanocrystal Zig-Zag Chains via In Situ TEM Imaging in Liquid.

Small 2020 May 27:e1907419. Epub 2020 May 27.

Istituto Italiano di Tecnologia, Via Morego 30, Genova, 16163, Italy.

The controlled assembly of colloidal magnetic nanocrystals is key to many applications such as nanoelectronics, storage memory devices, and nanomedicine. Here, the motion and ordering of ferrimagnetic nanocubes in water via liquid-cell transmission electron microscopy is directly imaged in situ. Through the experimental analysis, combined with molecular dynamics simulations and theoretical considerations, it is shown that the presence of highly competitive interactions leads to the formation of stable monomers and dimers, acting as nuclei, followed by a dynamic growth of zig-zag chain-like assemblies. Read More

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http://dx.doi.org/10.1002/smll.201907419DOI Listing