Artif Organs 2021 Apr 28. Epub 2021 Apr 28.
Zhongnan Hospital of Wuhan University, Institute of Hepatobiliary Diseases, Wuhan University, Transplant Center of Wuhan University, Hubei Key Laboratory of Medical Technology on Transplantation, Wuhan Hubei, 430071, PR China.
Background: In this study, we evaluated the restoring and defatting effect of hypothermic oxygenated perfusion (HOPE) on severe steatotic liver grafts with a defatting cocktail (DF) in a rat model.
Methods: Severe (≥60%) hepatic macrosteatosis was induced by a high-fat diet (HFD) for six weeks, after which the rats were randomly divided into four following groups: Control group, with lean livers being preserved in static cold storage (SCS) at 0-4℃ for 45 min; SCS group, with a steatotic liver graft (SLG) being preserved in SCS at 0-4℃ for 4 hours; HOPE group, where SLG was perfused with 3-h HOPE followed by 1-h SCS; HOPE+DF group, HOPE with the addition of DF. Graft function after orthotopic liver transplantation was assessed in terms of mitochondrial function (ATP, Glycogen), endoplasmic reticulum stress (PPY, GRP78, CHOP, and ATF-6), cell apoptosis (Tunel assay, Caspase-3), inflammatory level (HMGB1, TLR4, IL-1β, IL-6. Read More