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Skeletal Radiol 2019 Apr 17. Epub 2019 Apr 17.

Department of Radiology, Diagnóstico Médico Cantabria (DMC), Santander, Cantabria, Spain.

The ultrasound examination of hamstrings inspires respect due to the connective complexity of their structures, particularly for sonographers who are not used to this kind of study. Therefore, it is important to know the specific ultrasound reference points that facilitate the location of the hamstring structures, dividing them into four areas of interest: (a) tendinous origin of the hamstring, (b) the proximal half, (c) distal and medial half, and (d) distal and lateral half. The origin of the hamstrings is found at the level of the ischial tuberosity. Read More

J Food Prot 1989 Mar;52(3):173-177

USDA/ARS 2 , Roman L. Hruska U.S. Meat Animal Research Center, P.O. Box 166, Clay Center, NE 68933.

The attachment of bacteria ( Serratia marcescens , Staphylococcus aureus , Streptococcus faecalis , Salmonella arizonae , Pseudomonas aeruginosa , and Listeria monocytogenes ), to lean muscle tissue and fat tissue was investigated. The number of cells attached to the meat was directly proportional to the initial cell concentrations present. There was no significant difference in the number of cells attached between the lean muscle tissue and fat tissues among the organisms tested. Read More

Poult Sci 2019 Apr 17. Epub 2019 Apr 17.

Facultad de Medicina Veterinaria y de Zootecnia, Universidad Nacional de Colombia, Bogotá 11001, Colombia.

The gut microbiota has an important effect on poultry health and production. The aim of this study was to evaluate the effect of Colombian oregano (COO), Lippia origanoides Kunth, essential oil supplementation on broiler chicken performance and their cecal bacterial microbiome by 16S-based sequencing. Essential oil was extracted by steam distillation and analyzed by Gas chromatography/mass spectrometry. Read More

J Food Sci 2019 Apr 17. Epub 2019 Apr 17.

Food Science Research Unit, U.S. Dept. of Agriculture, Agricultural Research Service, SEA, North Carolina State Univ., 322 Schaub Hall, Box 7624, Raleigh, NC, 27695-7624, U.S.A.

Commercial fermentation for bulk preservation of cucumbers relies on natural microbiota and approximately 1 M sodium chloride (NaCl) brines, resulting in large volumes of high-salt wastewater. An alternative process utilizing 0.1 M calcium chloride (CaCl ) as the only salt was developed to eliminate NaCl from fermentation brines for reduced environmental impact. Read More

Commun Biol 2019 15;2:131. Epub 2019 Apr 15.

1Department of Cellular Biology and Pharmacology, Florida International University, Herbert Wertheim College of Medicine, Miami, FL 33199 USA.

The emergence and spread of antimicrobial resistance highlights the urgent need for new antibiotics. Organoarsenicals have been used as antimicrobials since Paul Ehrlich's salvarsan. Recently a soil bacterium was shown to produce the organoarsenical arsinothricin. Read More

Genet Med 2019 Apr 17. Epub 2019 Apr 17.

Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, USA.

Purpose: Maternal diabetes is a known teratogen that can cause a wide spectrum of birth defects, collectively referred to as diabetic embryopathy (DE). However, the pathogenic mechanisms underlying DE remain uncertain and there are no definitive tests to establish the diagnosis. Here, we explore the potential of DNA methylation as a diagnostic biomarker for DE and to inform disease pathogenesis. Read More

J Pediatr Surg 2019 Mar 28. Epub 2019 Mar 28.

Section of Pediatric Gastroenterology, Baylor College of Medicine, Houston, TX; USDA/ARS Children's Nutrition Research Center, Texas Children's Hospital, Houston, TX. Electronic address:

Background/purpose: Diagnostic delay or time to diagnosis, and its relationship with colectomy risk has been studied in adult Inflammatory Bowel Disease (IBD), but rarely in pediatric IBD (PIBD), especially pediatric ulcerative colitis (P-UC), which often has a more severe course than adult UC. This study compared the relationship between diagnostic delay and colectomy in P-UC.

Methods: The medical records of P-UC patients, ages <18 years, diagnosed at Texas Children's Hospital from 2012 to 2018 were examined. Read More

Int J Mol Sci 2019 Apr 15;20(8). Epub 2019 Apr 15.

USDA-ARS Dale Bumpers National Rice Research Center, Stuttgart, AR 72160, USA.

Access to adequate irrigation resources is critical for sustained agricultural production, and rice, a staple cereal grain for half of the world population, is one of the biggest users of irrigation. To reduce water use, several water saving irrigation systems have been developed for rice production, but a reliable system to evaluate cultivars for water stress tolerance is still lacking. Here, seven rice cultivars that have diverse yield potential under water stress were evaluated in a field study using four continuous irrigation regimes varying from saturation to wilting point. Read More

PLoS Genet 2019 Apr 16;15(4):e1007739. Epub 2019 Apr 16.

Division of Sleep and Circadian Disorders, Brigham and Women's Hospital, Boston, MA, United States of America.

Sleep disordered breathing (SDB)-related overnight hypoxemia is associated with cardiometabolic disease and other comorbidities. Understanding the genetic bases for variations in nocturnal hypoxemia may help understand mechanisms influencing oxygenation and SDB-related mortality. We conducted genome-wide association tests across 10 cohorts and 4 populations to identify genetic variants associated with three correlated measures of overnight oxyhemoglobin saturation: average and minimum oxyhemoglobin saturation during sleep and the percent of sleep with oxyhemoglobin saturation under 90%. Read More

Cell Stress Chaperones 2019 Apr 15. Epub 2019 Apr 15.

MOE Key Laboratory of Laser Life Science & College of Life Science, South China Normal University, Guangzhou, 510631, China.

Artesunate (ARS) induced significant reactive oxygen species (ROS) generation in HepG2, HeLa, and A549 lines. However, ARS induced ROS-dependent apoptosis in HeLa and A549 cell lines but ROS-independent apoptosis in HepG2 cells. A total of 200 μM hydrogen peroxide (HO) significantly induced cytotoxicity in HeLa cells, while HO up to 300 μM did not induce cytotoxicity in HepG2 cells, further demonstrating the strong resistance of HepG2 cells to ROS. Read More

Int J Vitam Nutr Res 2019 Apr 16:1-8. Epub 2019 Apr 16.

4 USDA, ARS, Western Human Nutrition Research Center, Davis, CA, USA.

Serum samples from the 1999 Mexico National Nutrition Survey (NNS) were analyzed to determine the prevalence of low serum B12 concentrations, identify factors related with low values including B12 intake, and importantly, to provide a baseline for monitoring progress in reducing deficiency. Samples for B12 were available from 488 children and 464 women, a sub-sample of the nationally representative 1999 NNS. The national overall prevalence of low (<200 pg/mL) and marginal (200 to 300 pg/mL) serum B12 was 25. Read More

Int J Vitam Nutr Res 2019 Apr 16:1-8. Epub 2019 Apr 16.

4 USDA, ARS, Western Human Nutrition Research Center, Davis, CA,USA.

Vitamin B12 (B12) plays in an important role in the development and function of the brain and nervous system, and adequate B12 status is especially important for the normal development of infants. In previous research conducted in Guatemala City we reported a high prevalence of B12 deficiency in lactating women and their infants 3 and 12 months of age, and low B12 concentrations in breast milk. The objective of this study was to assess predictors of serum B12 concentration in predominantly breastfed Guatemalan infants including intake of B12 from breast milk and other foods. Read More

Molecules 2019 Apr 4;24(7). Epub 2019 Apr 4.

State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A & F University, Yangling 712100, China.

Alkylresorcinols (ARs) are beneficial for human health and can be used as biomarkers for whole-grain cereal intake. In previous studies, ARs content in whole-grain flour was determined by milling the seeds into powder, thus destroying their structure. In this paper, we adopted a non-destructive extraction approach. Read More

Plants (Basel) 2019 Apr 2;8(4). Epub 2019 Apr 2.

DOE Center for Advanced Bioenergy and Bioproducts Innovation and Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.

Elevated tropospheric ozone concentration (O₃) increases oxidative stress in vegetation and threatens the stability of crop production. Current O₃ pollution in the United States is estimated to decrease the yields of maize () up to 10%, however, many bioenergy feedstocks including switchgrass () have not been studied for response to O₃ stress. Using Free Air Concentration Enrichment (FACE) technology, we investigated the impacts of elevated O₃ (~100 nmol mol) on leaf photosynthetic traits and capacity, chlorophyll fluorescence, the Ball⁻Woodrow⁻Berry (BWB) relationship, respiration, leaf structure, biomass and nutrient composition of switchgrass. Read More

Chem Biol Drug Des 2019 Apr 15. Epub 2019 Apr 15.

Faculty of Pharmacy, Philadelphia University-Jordan, P.O BOX (1), Philadelphia University, (19392), Jordan.

A series of novel 7-amino-5-oxo-2-substituted-aryl/heteroaryl-5,8-dihydro[1,2,4]triazolo[1,5-a]-pyridine-6-carbonitriles (4a-4t) was synthesized, characterized and evaluated for their binding affinity and selectivity towards hA , hA , hA and hA adenosine receptors (ARs). Compound 4a with a phenyl ring at 2-position of the triazolo moiety of the scaffold showed high affinity and selectivity for hA AR (K hA = 0.076 μM, hA = 25. Read More

J Anim Sci 2019 Apr 15. Epub 2019 Apr 15.

Department of Animal Sciences, University of Illinois, Urbana-Champaign, IL.

Adequate carcass chilling is required to optimize pork quality and food safety. The rate at which carcasses chill is dependent upon their mass. Hot carcass weight (HCW) has increased steadily over the years, certainly affecting the chilling rate of the average carcass in contemporary abattoirs. Read More

J Anim Sci 2019 Apr 15. Epub 2019 Apr 15.

West Texas A&M University, Canyon.

Wet distiller's grains with solubles (WDGS) is a common by-product feedstuff generated by the grain-ethanol industry, and it is used extensively by the cattle feeding industry. Distillers grains are typically high in protein; however, the protein in WDGS has a low ruminal degradability, and thus may result in a deficiency of rumen degradable protein (RDP) in the diet even when dietary crude protein (CP) concentrations are high. Assessment of the RDP needs in diets containing WDGS is needed to aid the cattle feeding industry in managing feed costs and potential environmental issues. Read More

Front Cell Neurosci 2019 28;13:124. Epub 2019 Mar 28.

Molecular Recognition Section, Laboratory of Bioorganic Chemistry, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, United States.

Adenosine receptors (ARs) function in the body's response to conditions of pathology and stress associated with a functional imbalance, such as in the supply and demand of energy/oxygen/nutrients. Extracellular adenosine concentrations vary widely to raise or lower the basal activation of four subtypes of ARs. Endogenous adenosine can correct an energy imbalance during hypoxia and other stress, for example, by slowing the heart rate by AAR activation or increasing the blood supply to heart muscle by the AAR. Read More

J Anim Sci 2019 Apr 15. Epub 2019 Apr 15.

West Texas A&M University, Canyon.

The use of distiller's grains (DG) in beef cattle finishing diets is a common practice. However, the effects of supplemental fat on performance and nutrient losses of cattle fed diets containing DG is not known. Therefore, we fed 398 crossbred yearling heifers (initial BW = 373. Read More

Turk Kardiyol Dern Ars 2019 Apr;47(3):232-234

Department of Cardiology, Kartal Koşuyolu Yüksek İhtisas Training and Research Hospital, İstanbul, Turkey.

Coronary artery bifurcation disease of a saphenous vein graft (SVG) is extremely rare. SVG disease remains a challenging lesion to treat because of increased morbidity and mortality with repeated coronary artery bypass graft (CABG) surgery, a high rate of periprocedural complications, and in-stent restenosis or occlusion requiring repeat revascularization with percutaneous coronary intervention. Presently described is use of the simultaneous kissing stent technique to treat inverted Y SVG bifurcation disease in a patient with a prior CABG and new-onset acute coronary syndrome. Read More

Turk Kardiyol Dern Ars 2019 Apr;47(3):162-167

Department of Cardiology, Yeditepe University Faculty of Medicine, İstanbul, Turkey.

diac structure and function in obese patients. This study was an examination of the short-term effects of sleeve gastrectomy on body measurements and diastolic function.

Methods: A total of 41 consecutive obese patients who were scheduled to undergo a sleeve gastrectomy procedure were included in the study. Read More

Turk Kardiyol Dern Ars 2019 Apr;47(3):243

Department of Cardiology,Tehran Heart Center, Tehran University of Medical Sciences, Tehran, I.R. Iran.

Turk Kardiyol Dern Ars 2019 Apr;47(3):245

Department of Cardiology, Adnan Menderes University Faculty of Medicine, Aydın, Turkey.

Turk Kardiyol Dern Ars 2019 Apr;47(3):239-242

Department of Radiology, Aspetar-Orthopaedic and Sports Medicine Hospital, Al Buwairda St, Doha, Qatar.

A patient with advanced rheumatic heart valve disease underwent aortic and mitral valve replacement with tricuspid ring annuloplasty. There was an anomalous left circumflex coronary artery (LCCA) arising from the right coronary artery (RCA) running along the anterior surface of an enlarged right ventricle (RV). During the immediate postoperative course, signs of inferior and lateral myocardial ischemia developed. Read More

Turk Kardiyol Dern Ars 2019 Apr;47(3):244

Gülhane Research and Education Hospital, Department of Cardiology, Turkey.

Turk Kardiyol Dern Ars 2019 Apr;47(3):191-197

Department of Cardiology, Türkiye Yüksek Ihtisas Training and Research Hospital, Ankara, Turkey.

Objective: Acute pulmonary embolism (APE) is a serious clinical situation and atrial fibrillation (AF) is the most common arrhytmia in clinical practice. The Pulmonary Embolism Severity Index (PESI) is an accepted risk stratification tool used to predict short term mortality in APE. The aim of this study was to evaluate the relationship between the PESI score and new-onset AF in patients with APE. Read More

Turk Kardiyol Dern Ars 2019 Apr;47(3):207-215

Dr. Sami Ulus Obstetrics and Gynecology, Children's Health and Diseases Training and Research Hospital, Ankara, Turkey.

Objective: Dilated cardiomyopathy (DCM) is a disorder featuring left ventricular dysfunction, heart failure, and a poor prognosis. The etiology is still unclear, despite diagnostic and therapeutic developments. This study was an evaluation of factors affecting the life span of a group of idiopathic DCM patients. Read More

Turk Kardiyol Dern Ars 2019 Apr;47(3):185-190

Department of Cardiology, Haseki Training and Research Hospital, İstanbul, Turkey.

Objective: Various hematological blood count parameters, including the neutrophil-to-lymphocyte ratio (NLR) and mean platelet volume (MPV), were analyzed to assess differences in patients with renovascular (RVH) and essential hypertension (EH).

Methods: A propensity score analysis was performed to match 51 patients with RVH and 173 patients with EH. After matching, 49 pairs of patients were compared. Read More

Turk Kardiyol Dern Ars 2019 Apr;47(3):228-231

Department of Cardiology, İstanbul Medipol University Faculty of Medicine, İstanbul, Turkey.

The implantation of aortic transcatheter heart valves has been successfully performed throughout the world in hundreds of patients with severe dysfunction of a degenerated mitral bioprosthesis or those at high surgical risk for re-operation. The transseptal approach may be more technically challenging, but is a less invasive procedure and may have a lower mortality rate compared with a transapical approach, and also offers a quick patient recovery. This report is a description of a rare case in Turkey: a successful transseptal mitral valve replacement in a case of a failed bioprosthetic valve. Read More

Turk Kardiyol Dern Ars 2019 Apr;47(3):198-206

Department of Cardiology, Faculty of Medicine, Hacettepe University, Ankara.

Objective: Heart failure (HF) is an important health issue of the 21st century and the prevalence in Turkey has been reported as 2.9%. A national profile, frequency data, characteristics of different phenotypes, and risk factors have not yet been well established. Read More

Turk Kardiyol Dern Ars 2019 Apr;47(3):246

Department Of Cardiology, Trakya University Faculty of Medicine, Edirne, Turkey.

Turk Kardiyol Dern Ars 2019 Apr;47(3):216-217

2Department of Cardiology, Kingston Health Sciences Centre, Queen's University, Kingston, Ontario, Canada.

Turk Kardiyol Dern Ars 2019 Apr;47(3):168-176

Department of Cardiology, Health Sciences University Adana Health Practices and Research Center, Adana.

Objective: The aim of this study was to investigate the relationship between ischemic changes in the lead aVR and left ventricular thrombus (LVT) or high-grade spontaneous echo contrast (SEC) in patients with acute anterior myocardial infarction (MI).

Methods: Quantitative T wave polarity in lead aVR (TPaVR) and ST segment deviation in the lead aVR (STaVR) measured from a surface electrocardiogram (ECG), as well as the absolute numerical values, were recorded. The ST/TPaVR ratio was obtained by dividing the larger absolute value by the smaller. Read More

Turk Kardiyol Dern Ars 2019 Apr;47(3):177-184

Department Of Cardiology, Tehran University Of Medical Sciences, Tehran, Iran.

Objective: Prolactin is correlated with some conditions that predispose individuals to atherosclerosis. Prolactin receptors have been found in atherosclerotic plaques. However, the correlation between the serum prolactin level and the extension and severity of coronary artery atherosclerosis has yet to be studied. Read More

Turk Kardiyol Dern Ars 2019 Apr;47(3):235-238

Department of Cardiology, Hacettepe University Faculty of Medicine, Ankara, Turkey.

Secondary hypertension accounts for 5% to 10% of all hypertensive cases, and renal artery stenosis is one of the most common causes of secondary hypertension. Although atherosclerotic vascular disease and fibromuscular dysplasia are the leading causes of renal artery stenosis, there are other, rare etiologies, such as vasculitis and trauma. A partial nephrectomy is the standard of care treatment option for early stage renal carcinoma patients. Read More

Turk Kardiyol Dern Ars 2019 Apr;47(3):159-161

Department of Clinical Biochemistry, Royal Free Hospital Campus, University College London Medical School, University College London (UCL), London, UK.

Turk Kardiyol Dern Ars 2019 Apr;47(3):218-227

Ankara University Faculty of Medicine Department of History of Medicine and Ethics, Ankara, Turkey.

Retrospective file research is very common in daily medical practice. Approval is required under current law for the review of personal genetic data, and the informed consent of all research subjects is necessary before conducting non-prospective research. In this article, the protection of personal data will be reviewed within the context of informed consent, the protection of confidentiality, the use of good anonymization techniques, research ethics, and medical law. Read More

J Nutr Biochem 2019 Feb 20;67:212-218. Epub 2019 Feb 20.

Cardiovascular Nutrition Laboratory, JM USDA Human Nutrition Research Center on Aging at Tufts University, Boston, MA. Electronic address:

Epicardial adipose tissue (EAT) inflammation is thought to potentiate the development of coronary artery disease (CAD). Overall diet quality and statin therapy are important modulators of inflammation and CAD progression. Our objective was to examine the effects and interaction of dietary patterns and statin therapy on EAT gene expression in the Ossabaw pig. Read More

Anim Genet 2019 Apr 12. Epub 2019 Apr 12.

Roman L. Hruska U.S. Meat Animal Research Center (USMARC), United States Department of Agriculture, Agricultural Research Service (USDA, ARS), Clay Center, NE, 68933, USA.

Previous work identified SNP associations with twinning rate in the US Holstein population and developed a model for genomic prediction. The current study was conducted to assess the association of these SNPs with twinning rate and ovulation rate in a genetically diverse, outbred population selected for twinning and ovulation rate. A total of 18 SNPs that were components of a prediction equation for twinning rate in Holstein cattle were genotyped on 731 animals from the USDA Meat Animal Research Center production efficiency or twinning population. Read More

Exp Clin Endocrinol Diabetes 2019 Apr 12. Epub 2019 Apr 12.

Department of Pharmacology and Biochemistry, Faculty of Pharmacy, Ankara University, Ankara, Turkey.

Aim: To investigate the possible beneficial effect of mirabegron [a selective β-adrenoceptor (AR) agonist] treatment on erectile dysfunction (ED) in streptozotocin-induced diabetic rats.

Methods: Sprague-Dawley rats (n=20) were divided into two groups: control group and streptozotocin-induced diabetic group. In vivo erectile responses were evaluated after intracavernosal injection of mirabegron (0. Read More

J Orthop Res 2019 Apr 12. Epub 2019 Apr 12.

Institute of Biomaterials and Biomedical Engineering, University of Toronto, Canada.

Osteocytes' mechano-regulation of bone formation and resorption is key to maintaining proper bone health. Although extensive in vitro studies have explored osteocyte mechanobiology using the well-established MLO-Y4 cell model, a low amount of sclerostin secreted by this cell line renders it inadequate for studying cross-talk between osteocytes and osteoblasts under mechanical loading. Here we investigated the potential of sclerostin-expressing OCY454 osteocyte cell model in fulfilling this role. Read More

Virus Genes 2019 Apr 12. Epub 2019 Apr 12.

Southeast Poultry Research Laboratory, US National Poultry Research Center, 934 College Station Rd, Athens, GA, 30605, USA.

Virulent Newcastle disease viruses (NDV) have been present in Mexico since 1946, and recently, multiple outbreaks have been reported in the country. Here, we characterized eleven NDV isolated from apparently healthy wild birds and backyard chickens in three different locations of Jalisco, Mexico in 2017. Total RNA from NDV was reverse-transcribed, and 1285 nucleotides, which includes 3/4 of the fusion gene, was amplified and sequenced using a long-read MinION sequencing method. Read More

Evol Appl 2019 Apr 31;12(4):815-829. Epub 2019 Jan 31.

Department of Entomology University of California Riverside California.

Parasitic wasps are among the most species-rich groups on Earth. A major cause of this diversity may be local adaptation to host species. However, little is known about variation in host specificity among populations within parasitoid species. Read More

Evol Appl 2019 Apr 4;12(4):773-790. Epub 2019 Jan 4.

Department of Biological Sciences University of Southern California Los Angeles California.

The intentional introduction of exotic species through classical biological control programs provides unique opportunities to examine the consequences of population movement and ecological processes for the genetic diversity and population structure of introduced species. The weevils and (Coleoptera: Curculionidae) have been introduced globally to control the invasive floating aquatic weed, , with variable outcomes. Here, we use the importation history and data from polymorphic microsatellite markers to examine the effects of introduction processes on population genetic diversity and structure. Read More

Evol Appl 2019 Apr 31;12(4):744-756. Epub 2018 Dec 31.

Crop and Soil Sciences, College of Agricultural and Environmental Sciences University of Georgia Tifton Georgia.

In biological control, populations of both the biological control agent and the pest have the potential to evolve and even to coevolve. This feature marks the most powerful and unpredictable aspect of biological control strategies. In particular, evolutionary change in host specificity of the biological control agent could increase or decrease its efficacy. Read More

BMC Res Notes 2019 Apr 11;12(1):223. Epub 2019 Apr 11.

Maryland Institute for Applied Environmental Health, University of Maryland School of Public Health, School of Public Health Building (255), 4200 Valley Drive, Room 2234P, College Park, MD, 20742, USA.

Objective: Zero-valent iron sand filtration can remove multiple contaminants, including some types of pathogenic bacteria, from contaminated water. However, its efficacy at removing complex viral populations, such as those found in reclaimed water used for agricultural irrigation, has not been fully evaluated. Therefore, this study utilized metagenomic sequencing and epifluorescent microscopy to enumerate and characterize viral populations found in reclaimed water and zero-valent iron-sand filtered reclaimed water sampled three times during a larger greenhouse study. Read More

BMC Nephrol 2019 Apr 11;20(1):126. Epub 2019 Apr 11.

Molecular Biology Laboratory, Fundació Puigvert, Instituto de Investigaciones Biomédicas Sant Pau (IIB-Sant Pau), Universitat Autònoma de Barcelona, REDinREN, Instituto de Investigación Carlos III, Cartagena 340-350, 08025, Barcelona, Catalonia, Spain.

Background: Galloway-Mowat syndrome (GAMOS) is a rare autosomal recessive disorder characterized by early-onset nephrotic syndrome and microcephaly with brain anomalies. WDR73 pathogenic variants were described as the first genetic cause of GAMOS and, very recently, four novel causative genes, OSGEP, LAGE3, TP53RK, and TPRKB, have been identified.

Case Presentation: We present the clinical and genetic characteristics of two unrelated infants with clinical suspicion of GAMOS who were born from consanguineous parents. Read More

J Virol Methods 2019 Apr 8;269:70-76. Epub 2019 Apr 8.

Arthropod-Borne Animal Diseases Research Unit, USDA, ARS, Manhattan, KS, USA.

Rift Valley fever virus (RVFV) is an important mosquito-borne pathogen with devastating impacts on agriculture and public health. With outbreaks being reported beyond the continent of Africa to the Middle East, there is great concern that RVFV will continue to spread to non-endemic areas such as the Americas and Europe. There is a need for safe and high throughput serological assays for rapid detection of RVFV during outbreaks and for surveillance. Read More

Genomics 2019 Apr 8. Epub 2019 Apr 8.

Food and Drug Administration, Center for Food Safety and Applied Nutrition, College Park, MD 20740.

A total of 91 draft genome sequences were used to analyze isolates of Salmonella enterica serovar Enteritidis obtained from feral mice caught on poultry farms in Pennsylvania. One objective was to find mutations disrupting open reading frames (ORFs) and another was to determine if ORF-disruptive mutations were present in isolates obtained from other sources. A total of 83 mice were obtained between 1995-1998. Read More

Dev Comp Immunol 2019 Apr 8;98:20-33. Epub 2019 Apr 8.

U.S. Department of Agriculture - Agriculture Research Service, Beltsville Human Nutrition Research Center, Diet, Genomics and Immunology Laboratory, Beltsville, MD, 20705, USA. Electronic address:

We previously demonstrated that the most bioactive vitamin A metabolite, all-trans retinoic acid (ATRA), increased T helper 2-associated responses induced in pigs by infection with the parasitic nematode Ascaris suum We also showed that ATRA potentiated the mRNA expression of several IL-4 induced chemokines (chemokine (CC motif) ligand 11 [(CCL11), CCL17, CCL22 and CCL26] associated with alternative activation (M2a) in porcine macrophages in vitro. Herein, several mechanisms whereby ATRA affects IL-4 signaling are profiled using large-scale real time PCR and RNA-Seq analysis. Twenty-three genes associated with M2a markers in other species were independently upregulated by both IL-4 and ATRA, including the adenosine receptor A2B (ADORA2B), cysteinyl leukotriene receptor 2 (CYSLTR2) and the vitamin D receptor (VDR). Read More