4,384 results match your criteria Arenaviruses


Confocal Imaging of Double-Stranded RNA and Pattern Recognition Receptors in Negative-Sense RNA Virus Infection.

J Vis Exp 2019 Jan 26(143). Epub 2019 Jan 26.

Department of Pathology and Institute for Human Infections and Immunity, University of Texas Medical Branch.

Double-stranded (ds) RNA is produced as a replicative intermediate during RNA virus infection. Recognition of dsRNA by host pattern recognition receptors (PRRs) such as the retinoic acid (RIG-I) like receptors (RLRs) RIG-I and melanoma differentiation-associated protein 5 (MDA-5) leads to the induction of the innate immune response. The formation and intracellular distribution of dsRNA in positive-sense RNA virus infection has been well characterized by microscopy. Read More

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http://dx.doi.org/10.3791/59095DOI Listing
January 2019
2 Reads

TRIM2, a novel member of the antiviral family, limits New World arenavirus entry.

PLoS Biol 2019 Feb 6;17(2):e3000137. Epub 2019 Feb 6.

Department of Microbiology and Immunology, UIC College of Medicine, Chicago, Illinois, United States of America.

Tripartite motif (TRIM) proteins belong to a large family with many roles in host biology, including restricting virus infection. Here, we found that TRIM2, which has been implicated in cases of Charcot-Marie-Tooth disease (CMTD) in humans, acts by blocking hemorrhagic fever New World arenavirus (NWA) entry into cells. We show that Trim2-knockout mice, as well as primary fibroblasts from a CMTD patient with mutations in TRIM2, are more highly infected by the NWAs Junín and Tacaribe virus than wild-type mice or cells are. Read More

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http://dx.doi.org/10.1371/journal.pbio.3000137DOI Listing
February 2019
1 Read

Virus⁻Host Interactions Involved in Lassa Virus Entry and Genome Replication.

Pathogens 2019 Jan 29;8(1). Epub 2019 Jan 29.

Centro de Virología Animal (CEVAN), CONICET-SENASA, Av Sir Alexander Fleming 1653, Martínez, Provincia de Buenos Aires B1640CSI, Argentina.

Lassa virus (LASV) is the causative agent of Lassa fever, a human hemorrhagic disease associated with high mortality and morbidity rates, particularly prevalent in West Africa. Over the past few years, a significant amount of novel information has been provided on cellular factors that are determinant elements playing a role in arenavirus multiplication. In this review, we focus on host proteins that intersect with the initial steps of the LASV replication cycle: virus entry and genome replication. Read More

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http://www.mdpi.com/2076-0817/8/1/17
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http://dx.doi.org/10.3390/pathogens8010017DOI Listing
January 2019
3 Reads

Assessing cross-reactivity of Junín virus-directed neutralizing antibodies.

Antiviral Res 2019 Mar 19;163:106-116. Epub 2019 Jan 19.

Junior Research Group Arenavirus Biology, Friedrich-Loeffler-Institut, Greifswald, Insel Riems, Germany; Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Hamilton, MT, USA. Electronic address:

Arenaviruses cause several viral hemorrhagic fevers endemic to Africa and South America. The respective causative agents are classified as biosafety level (BSL) 4 pathogens. Unlike for most other BSL4 agents, for the New World arenavirus Junín virus (JUNV) both a highly effective vaccination (Candid#1) and a post-exposure treatment, based on convalescent plasma transfer, are available. Read More

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http://dx.doi.org/10.1016/j.antiviral.2019.01.006DOI Listing
March 2019
1 Read

Lassa Fever in Travelers from West Africa, 1969-2016.

Emerg Infect Dis 2019 Feb;25(2):245-248

Lassa virus is a rodentborne arenavirus responsible for human cases of Lassa fever, a viral hemorrhagic fever, in West Africa and in travelers arriving to non-Lassa-endemic countries from West Africa. We describe a retrospective review performed through literature search of clinical and epidemiologic characteristics of all imported Lassa fever cases worldwide during 1969-2016. Our findings demonstrate that approximately half of imported cases had distinctive clinical features (defined as fever and >1 of the following: pharyngitis, sore throat, tonsillitis, conjunctivitis, oropharyngeal ulcers, or proteinuria). Read More

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http://dx.doi.org/10.3201/eid2502.180836DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6346466PMC
February 2019
1 Read

Attenuated Replication of Lassa Virus Vaccine Candidate ML29 in STAT-1 Mice.

Pathogens 2019 Jan 15;8(1). Epub 2019 Jan 15.

Department of Pharmacology and Toxicology, University of Louisville Health Sciences Center, Louisville, KY 40292, USA.

Lassa virus (LASV), a highly prevalent mammalian arenavirus endemic in West Africa, can cause Lassa fever (LF), which is responsible for thousands of deaths annually. LASV is transmitted to humans from naturally infected rodents. At present, there is not an effective vaccine nor treatment. Read More

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http://www.mdpi.com/2076-0817/8/1/9
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http://dx.doi.org/10.3390/pathogens8010009DOI Listing
January 2019
8 Reads

Expression of novel long noncoding RNAs defines virus-specific effector and memory CD8 T cells.

Nat Commun 2019 01 14;10(1):196. Epub 2019 Jan 14.

Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, GA, 30307, USA.

In response to viral infection, CD8 T cells undergo expansion and differentiate into distinct classes of effector cells. After clearance of the virus, a small population of long-lived memory cells persists. Comprehensive studies have defined the protein-coding transcriptional changes associated with this process. Read More

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http://dx.doi.org/10.1038/s41467-018-07956-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6331603PMC
January 2019

Identification of clotrimazole-derivatives as specific inhibitors of Arenavirus fusion.

J Virol 2019 Jan 9. Epub 2019 Jan 9.

Institute of Microbiology, Lausanne University Hospital, Lausanne, Switzerland

Arenaviruses are a large family of emerging enveloped negative strand RNA viruses that include several causative agents of viral hemorrhagic fevers. For cell entry, human pathogenic arenaviruses use different cellular receptors and endocytic pathways that converge at the level of acidified late endosomes, where the viral envelope glycoprotein mediates membrane fusion. Inhibitors of arenavirus entry hold promise for therapeutic antiviral intervention and the identification of "druggable" targets is of high priority. Read More

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http://jvi.asm.org/lookup/doi/10.1128/JVI.01744-18
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http://dx.doi.org/10.1128/JVI.01744-18DOI Listing
January 2019
5 Reads

A mini-genome study of Hazara Nairovirus genomic promoters.

J Virol 2019 Jan 9. Epub 2019 Jan 9.

Department of Microbiology, School of Medicine, Wakayama Medical University, Japan.

Hazara Nairovirus (HAZV) is a tri-segmented RNA virus most closely related to Crimean-Congo hemorrhagic fever virus, in the order The terminal roughly 20 nt of their genome ends are highly complementary, similar to those of other segmented negative-strand RNA viruses (sNSV), and act as promoters for RNA synthesis. These promoters contain two elements; the very termini of both strands (promoter element 1 (PE1)) are conserved and virus-specific, and are found bound to separate sites on the polymerase surface in crystal structures of promoter-polymerase complexes. The following sequences (PE2) are segment-specific with the potential to form dsRNA, and this latter aspect is also important for promoter activity. Read More

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http://dx.doi.org/10.1128/JVI.02118-18DOI Listing
January 2019

Understanding Lassa fever.

Authors:
Nahid Bhadelia

Science 2019 01;363(6422):30

Section of Infectious Diseases, Boston University School of Medicine and National Emerging Infectious Diseases Laboratories, Boston University, Boston, MA, USA.

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http://dx.doi.org/10.1126/science.aav8958DOI Listing
January 2019

Identification of Residues in Lassa Virus Glycoprotein Subunit 2 That Are Critical for Protein Function.

Pathogens 2018 Dec 26;8(1). Epub 2018 Dec 26.

Department of Infectious Diseases, Department of Population Health, Center for Vaccines and Immunology, College of Veterinary Medicine, University of Georgia, Athens, GA 30602, USA.

Lassa virus (LASV) is an Old World arenavirus, endemic to West Africa, capable of causing hemorrhagic fever. Currently, there are no approved vaccines or effective antivirals for LASV. However, thorough understanding of the LASV glycoprotein and entry into host cells could accelerate therapeutic design. Read More

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http://www.mdpi.com/2076-0817/8/1/1
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http://dx.doi.org/10.3390/pathogens8010001DOI Listing
December 2018
4 Reads

A potent Lassa virus antiviral targets an arenavirus virulence determinant.

PLoS Pathog 2018 12 21;14(12):e1007439. Epub 2018 Dec 21.

Kineta, Inc., Seattle, Washington, United States of America.

Arenaviruses are a significant cause of hemorrhagic fever, an often-fatal disease for which there is no approved antiviral therapy. Lassa fever in particular generates high morbidity and mortality in West Africa, where the disease is endemic, and a recent outbreak in Nigeria was larger and more geographically diverse than usual. We are developing LHF-535, a small-molecule viral entry inhibitor that targets the arenavirus envelope glycoprotein, as a therapeutic candidate for Lassa fever and other hemorrhagic fevers of arenavirus origin. Read More

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http://dx.doi.org/10.1371/journal.ppat.1007439DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6322784PMC
December 2018
11 Reads

Report of a fatal case of Lassa fever in Parakou in 2018: clinical, therapeutic and diagnostic aspects.

BMC Infect Dis 2018 Dec 17;18(1):667. Epub 2018 Dec 17.

Medicine and Medical Specialities Department, Faculty of Medicine, University of Parakou, P. O Box 123, Parakou, Republic of Benin.

Background: Lassa fever is one of the most lethal neglected tropical diseases in West Africa. It is a serious public health problem in this region of Africa where it is endemic in several countries. However, it remains a very little known disease by healthcare workers. Read More

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http://dx.doi.org/10.1186/s12879-018-3587-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6296101PMC
December 2018
1 Read

Metal chelators for the inhibition of the lymphocytic choriomeningitis virus endonuclease domain.

Antiviral Res 2019 Feb 14;162:79-89. Epub 2018 Dec 14.

Aix-Marseille Université, CNRS UMR 7257, Architecture et Fonction des Macromolécules Biologiques, 163 avenue de Luminy, 13288, Marseille, France. Electronic address:

Arenaviridae is a viral family whose members are associated with rodent-transmitted infections to humans responsible of severe diseases. The current lack of a vaccine and limited therapeutic options make the development of efficacious drugs of high priority. The cap-snatching mechanism of transcription of Arenavirus performed by the endonuclease domain of the L-protein is unique and essential, so we developed a drug design program targeting the endonuclease activity of the prototypic Lymphocytic ChorioMeningitis Virus. Read More

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http://dx.doi.org/10.1016/j.antiviral.2018.12.008DOI Listing
February 2019
2 Reads

LARGE expression in different types of muscular dystrophies other than dystroglycanopathy.

BMC Neurol 2018 Dec 15;18(1):207. Epub 2018 Dec 15.

Department of Medical Biology, Hacettepe University Faculty of Medicine, 06100 Sihhiye, Ankara, Turkey.

Background: Alpha-dystroglycan (αDG) is an extracellular peripheral glycoprotein that acts as a receptor for both extracellular matrix proteins containing laminin globular domains and certain arenaviruses. An important enzyme, known as Like-acetylglucosaminyltransferase (LARGE), has been shown to transfer repeating units of -glucuronic acid-β1,3-xylose-α1,3- (matriglycan) to αDG that is required for functional receptor as an extracellular matrix protein scaffold. The reduction in the amount of LARGE-dependent matriglycan result in heterogeneous forms of dystroglycanopathy that is associated with hypoglycosylation of αDG and a consequent lack of ligand-binding activity. Read More

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https://bmcneurol.biomedcentral.com/articles/10.1186/s12883-
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http://dx.doi.org/10.1186/s12883-018-1207-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6295086PMC
December 2018
14 Reads

Host-Driven Phosphorylation Appears to Regulate the Budding Activity of the Lassa Virus Matrix Protein.

Pathogens 2018 Dec 9;7(4). Epub 2018 Dec 9.

Department of Medicine, Division of Immunobiology, University of Vermont, Burlington, VT 05405, USA.

Lassa mammarenavirus (LASV) is an enveloped RNA virus that can cause Lassa fever, an acute hemorrhagic fever syndrome associated with significant morbidity and high rates of fatality in endemic regions of western Africa. The arenavirus matrix protein Z has several functions during the virus life cycle, including coordinating viral assembly, driving the release of new virus particles, regulating viral polymerase activity, and antagonizing the host antiviral response. There is limited knowledge regarding how the various functions of Z are regulated. Read More

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http://dx.doi.org/10.3390/pathogens7040097DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6313517PMC
December 2018

Genetic characterization of Lassa virus strains isolated from 2012 to 2016 in southeastern Nigeria.

PLoS Negl Trop Dis 2018 11 30;12(11):e0006971. Epub 2018 Nov 30.

Department of Emerging Infectious Diseases, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Nagasaki, Japan.

Lassa virus (LASV) is endemic in parts of West Africa where it causes Lassa fever (LF), a viral hemorrhagic fever with frequent fatal outcomes. The diverse LASV strains are grouped into six major lineages based on the geographical location of the isolated strains. In this study, we have focused on the lineage II strains from southern Nigeria. Read More

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http://dx.doi.org/10.1371/journal.pntd.0006971DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6267959PMC
November 2018
1 Read

Arenavirus genomics: novel insights into viral diversity, origin, and evolution.

Curr Opin Virol 2018 Nov 26;34:18-28. Epub 2018 Nov 26.

Scientific Institute, IRCCS E. MEDEA, Bioinformatics, 23842 Bosisio Parini, Lecco, Italy. Electronic address:

Next-generation sequencing technologies have revolutionized our knowledge of virus diversity and evolution. In the case of arenaviruses, which are the focus of this review, metagenomic/metatranscriptomic approaches identified reptile-infecting and fish-infecting viruses, also showing that bi-segmented genomes are not a universal feature of the Arenaviridae family. Novel mammarenaviruses were described, allowing inference of their geographic origin and evolutionary dynamics. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S18796257183010
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http://dx.doi.org/10.1016/j.coviro.2018.11.001DOI Listing
November 2018

Recombinant Lassa Virus Expressing Green Fluorescent Protein as a Tool for High-Throughput Drug Screens and Neutralizing Antibody Assays.

Viruses 2018 11 20;10(11). Epub 2018 Nov 20.

Integrated Research Facility at Fort Detrick (IRF-Frederick), National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), B-8200 Research Plaza, Fort Detrick, Frederick, MD 21702, USA.

Lassa virus (LASV), a mammarenavirus, infects an estimated 100,000⁻300,000 individuals yearly in western Africa and frequently causes lethal disease. Currently, no LASV-specific antivirals or vaccines are commercially available for prevention or treatment of Lassa fever, the disease caused by LASV. The development of medical countermeasure screening platforms is a crucial step to yield licensable products. Read More

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http://dx.doi.org/10.3390/v10110655DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6266387PMC
November 2018
18 Reads

Characterization of Haartman Institute snake virus-1 (HISV-1) and HISV-like viruses-The representatives of genus Hartmanivirus, family Arenaviridae.

PLoS Pathog 2018 11 14;14(11):e1007415. Epub 2018 Nov 14.

Institute of Veterinary Pathology, Vetsuisse Faculty, University of Zurich, Zurich, Switzerland.

The family Arenaviridae comprises three genera, Mammarenavirus, Reptarenavirus and the most recently added Hartmanivirus. Arenaviruses have a bisegmented genome with ambisense coding strategy. For mammarenaviruses and reptarenaviruses the L segment encodes the Z protein (ZP) and the RNA-dependent RNA polymerase, and the S segment encodes the glycoprotein precursor and the nucleoprotein. Read More

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http://dx.plos.org/10.1371/journal.ppat.1007415
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http://dx.doi.org/10.1371/journal.ppat.1007415DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6261641PMC
November 2018
8 Reads

Lassa virus activates myeloid dendritic cells but suppresses their ability to stimulate T cells.

PLoS Pathog 2018 11 12;14(11):e1007430. Epub 2018 Nov 12.

Unité de Biologie des Infections Virales Emergentes, Institut Pasteur; Centre International de Recherche en Infectiologie (INSERM, CNRS, ENS Lyon, Université Lyon I), Lyon, France.

Lassa virus (LASV) is responsible for a viral hemorrhagic fever in humans and the death of 3,000 to 5,000 people every year. The immune response to LASV is poorly understood, but type I interferon (IFN-I) and T-cell responses appear to be critical for the host. We studied the response of myeloid dendritic cells (mDC) to LASV, as mDCs are involved in both IFN-I production and T-cell activation. Read More

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http://dx.doi.org/10.1371/journal.ppat.1007430DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6258464PMC
November 2018

Rodent control to fight Lassa fever: Evaluation and lessons learned from a 4-year study in Upper Guinea.

PLoS Negl Trop Dis 2018 11 6;12(11):e0006829. Epub 2018 Nov 6.

Department of Virology, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.

Lassa fever is a viral haemorrhagic fever caused by an arenavirus. The disease is endemic in West African countries, including Guinea. The rodents Mastomys natalensis and Mastomys erythroleucus have been identified as Lassa virus reservoirs in Guinea. Read More

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http://dx.plos.org/10.1371/journal.pntd.0006829
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http://dx.doi.org/10.1371/journal.pntd.0006829DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6219765PMC
November 2018
6 Reads

Tangeretin, an extract from Citrus peels, blocks cellular entry of arenaviruses that cause viral hemorrhagic fever.

Antiviral Res 2018 Dec 16;160:87-93. Epub 2018 Oct 16.

State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China; Department of Pharmacology, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100050, China. Electronic address:

The family Arenaviridae consists of numerous enveloped RNA viruses with ambisense coding strategies. Eight arenaviruses, including Lassa virus, are known to cause severe and fatal viral hemorrhagic fever (VHF) in humans, yet vaccines and treatments for disease caused by arenaviruses are very limited. In this study, we screened a natural product library consisting of 131 compounds and identified tangeretin, a polymethoxylated flavone widely present in citrus fruit peels, as a Lassa virus entry inhibitor that blocks viral fusion. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01663542183040
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http://dx.doi.org/10.1016/j.antiviral.2018.10.011DOI Listing
December 2018
15 Reads

Genomic Analysis of Lassa Virus during an Increase in Cases in Nigeria in 2018.

N Engl J Med 2018 11 17;379(18):1745-1753. Epub 2018 Oct 17.

From the Broad Institute of the Massachusetts Institute of Technology (MIT) and Harvard University (K.J.S., K.G.B., S.M., S.F.S., S.M.W., R.R.S., J.Q., S.W., P.B., S.Y., B.C., D.K., A.C., A.G.-Y., C.A.F., D.J.P., N.L.Y., B.L.M., P.C.S.), the Center for Systems Biology, Department of Organismic and Evolutionary Biology (K.J.S., K.G.B., S.F.S., S.W., B.C., D.K., C.A.F., N.L.Y., P.C.S.), and the Faculty of Arts and Sciences (M.N.), Harvard University, Harvard University Extension School (R.R.S.), and Harvard-MIT Health Sciences and Technology, MIT (S.Y., P.C.S.), Cambridge, and the Department of Immunology and Infectious Diseases, Harvard T.H. Chan School of Public Health (K.G.B., S.F.S., B.L.M., P.C.S., C.T.H.), and Beth Israel Deaconess Medical Center, Division of Infectious Diseases (S.M.), Boston - all in Massachusetts; the African Center of Excellence for Genomics of Infectious Diseases (P.E., J.U.O., E.U., T.K., F.A., J.U., A.G., M.M., I.N., P.O., O.T., O.A.F., C.T.H.) and the Department of Biological Sciences, College of Natural Sciences (J.U.O., E.U., T.K., F.A., J.U., P.O., O.A.F., C.T.H.), Redeemer's University, Ede, the Institute of Lassa Fever Research and Control (I.O., C. Iruolagbe, J.A., E.U., P.A., G.O., O.O., B.O., E.B.M., M.A., R.E., B.E., E.O.-E., G.A., S.O., P.O.O., C.T.H.) and the Department of Medicine (P.O.O.), Irrua Specialist Teaching Hospital, Irrua, the Nigeria Center for Disease Control, Abuja (C. Ihekweazu), and the Department of Medicine, Faculty of Clinical Sciences, Ambrose Alli University, Ekpoma (P.O.O.) - all in Nigeria; the Laboratory of Parasitology/Mycology HALD, Cheikh Anta Diop University of Dakar, Dakar, Senegal (A.G.); Kenema Government Hospital, Kenema, Sierra Leone (M.M.); Tulane Health Sciences Center, Tulane University, New Orleans (R.F.G.); the Departments of Immunology and Microbial Science and Integrative Structural and Computational Biology, Scripps Research Institute, and the Scripps Translational Science Institute, La Jolla, CA (K.G.A.); and Howard Hughes Medical Institute, Chevy Chase, MD (P.C.S.).

During 2018, an unusual increase in Lassa fever cases occurred in Nigeria, raising concern among national and international public health agencies. We analyzed 220 Lassa virus genomes from infected patients, including 129 from the 2017-2018 transmission season, to understand the viral populations underpinning the increase. A total of 14 initial genomes from 2018 samples were generated at Redeemer's University in Nigeria, and the findings were shared with the Nigerian Center for Disease Control in real time. Read More

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http://dx.doi.org/10.1056/NEJMoa1804498DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6181183PMC
November 2018
27 Reads
55.873 Impact Factor

Lymphocytic choriomeningitis virus meningoencephalitis in a renal transplant recipient following exposure to mice.

Transpl Infect Dis 2018 Dec 5;20(6):e13013. Epub 2018 Nov 5.

Division of Infectious Disease, St. John Hospital and Medical Center, Grosse Pointe Woods, Michigan.

Solid organ transplant recipients (SOTR) are at increased risk for a wide variety of typical and atypical infections as a consequence of impaired cell mediated and humoral immunity. We report a case of meningoencephalitis in a renal transplant recipient caused by lymphocytic choriomeningitis virus (LCMV) acquired by exposure to mice excreta. The clinical course was complicated by the development of hydrocephalus, requiring a ventriculoperitoneal shunt. Read More

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http://dx.doi.org/10.1111/tid.13013DOI Listing
December 2018
2 Reads

Does exploratory behavior or activity in a wild mouse explain susceptibility to virus infection?

Curr Zool 2018 Oct 12;64(5):585-592. Epub 2017 Sep 12.

Evolutionary Ecology Group, Department of Biology, University of Antwerp, Antwerp, Belgium.

Exploration and activity are often described as trade-offs between the fitness benefits of gathering information and resources, and the potential costs of increasing exposure to predators and parasites. More exploratory individuals are predicted to have higher rates of parasitism, but this relationship has rarely been examined for virus infections in wild populations. Here, we used the multimammate mouse to investigate the relationship between exploration, activity, and infection with Morogoro virus (MORV). Read More

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https://academic.oup.com/cz/article/64/5/585/4139746
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http://dx.doi.org/10.1093/cz/zox053DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6178786PMC
October 2018
8 Reads
1.814 Impact Factor

Non-neutralizing antibodies elicited by recombinant Lassa-Rabies vaccine are critical for protection against Lassa fever.

Nat Commun 2018 10 11;9(1):4223. Epub 2018 Oct 11.

Department of Microbiology and Immunology, Sidney Kimmel Medical College at Thomas Jefferson University, Philadelphia, PA, 19107, USA.

Lassa fever (LF), caused by Lassa virus (LASV), is a viral hemorrhagic fever for which no approved vaccine or potent antiviral treatment is available. LF is a WHO priority disease and, together with rabies, a major health burden in West Africa. Here we present the development and characterization of an inactivated recombinant LASV and rabies vaccine candidate (LASSARAB) that expresses a codon-optimized LASV glycoprotein (coGPC) and is adjuvanted by a TLR-4 agonist (GLA-SE). Read More

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http://www.nature.com/articles/s41467-018-06741-w
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http://dx.doi.org/10.1038/s41467-018-06741-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6181965PMC
October 2018
2 Reads

Structure-Based Classification Defines the Discrete Conformational Classes Adopted by the Arenaviral GP1.

J Virol 2019 Jan 10;93(1). Epub 2018 Dec 10.

Division of Structural Biology, Wellcome Centre for Human Genetics, University of Oxford, Oxford, United Kingdom

The emergence of Old and New World arenaviruses from rodent reservoirs persistently threatens human health. The GP1 subunit of the envelope-displayed arenaviral glycoprotein spike complex (GPC) mediates host cell recognition and is an important determinant of cross-species transmission. Previous structural analyses of Old World arenaviral GP1 glycoproteins, alone and in complex with a cognate GP2 subunit, have revealed that GP1 adopts two distinct conformational states distinguished by differences in the orientations of helical regions of the molecule. Read More

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http://jvi.asm.org/lookup/doi/10.1128/JVI.01048-18
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http://dx.doi.org/10.1128/JVI.01048-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6288339PMC
January 2019
1 Read

Bacterial coinfection restrains antiviral CD8 T-cell response via LPS-induced inhibitory NK cells.

Nat Commun 2018 10 8;9(1):4117. Epub 2018 Oct 8.

Institute for Immunology, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, 79104 Freiburg, Germany.

Infection of specific pathogen-free mice with lymphocytic choriomeningitis virus (LCMV) is a widely used model to study antiviral T-cell immunity. Infections in the real world, however, are often accompanied by coinfections with unrelated pathogens. Here we show that in mice, systemic coinfection with E. Read More

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http://www.nature.com/articles/s41467-018-06609-z
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http://dx.doi.org/10.1038/s41467-018-06609-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6175863PMC
October 2018
11 Reads

Critical role for cholesterol in Lassa fever virus entry identified by a novel small molecule inhibitor targeting the viral receptor LAMP1.

PLoS Pathog 2018 09 28;14(9):e1007322. Epub 2018 Sep 28.

Division of Hematology, Department of Medicine, Brigham and Women's Hospital, Boston, Massachusetts, United States of America.

Lassa fever virus (LASV) is endemic in West Africa and causes severe hemorrhagic fever and sensorineural hearing loss. We identified a small molecule inhibitor of LASV and used it to analyze the mechanism of entry. Using a photo-reactive analog that retains antiviral activity as a probe, we identified the inhibitor target as lysosome-associated membrane protein 1 (LAMP1), a host factor that binds to the LASV glycoprotein (GP) during infection. Read More

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http://dx.plos.org/10.1371/journal.ppat.1007322
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http://dx.doi.org/10.1371/journal.ppat.1007322DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6179309PMC
September 2018
15 Reads

Lupus acceleration by a MAVS-activating RNA virus requires endosomal TLR signaling and host genetic predisposition.

PLoS One 2018 10;13(9):e0203118. Epub 2018 Sep 10.

Department of Immunology and Microbiology, The Scripps Research Institute, La Jolla, California, United States of America.

Viruses have long been implicated in the pathogenesis of autoimmunity, yet their contribution remains circumstantial partly due to the lack of well-documented information on infections prior to autoimmune disease onset. Here, we used the lymphocytic choriomeningitis virus (LCMV) as a model to mechanistically dissect the impact of viral infection on lupus-like autoimmunity. Virus persistence strongly enhanced disease in mice with otherwise weak genetic predisposition but not in highly predisposed or non-autoimmune mice, indicating a synergistic interplay between genetic susceptibility and virus infection. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0203118PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6130858PMC
February 2019
7 Reads

Structural basis for receptor recognition by Lujo virus.

Nat Microbiol 2018 Oct 27;3(10):1153-1160. Epub 2018 Aug 27.

Department of Structural Biology, Weizmann Institute of Science, Rehovot, Israel.

Lujo virus (LUJV) has emerged as a highly fatal human pathogen. Despite its membership among the Arenaviridae, LUJV does not classify with the known Old and New World groups of that viral family. Likewise, LUJV was recently found to use neuropilin-2 (NRP2) as a cellular receptor instead of the canonical receptors used by Old World and New World arenaviruses. Read More

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http://dx.doi.org/10.1038/s41564-018-0224-5DOI Listing
October 2018

Current research for a vaccine against Lassa hemorrhagic fever virus.

Drug Des Devel Ther 2018 14;12:2519-2527. Epub 2018 Aug 14.

Zoonotic Diseases and Special Pathogens Program, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB, Canada,

Lassa virus (LASV) is a rodent-borne arenavirus endemic to several West African countries that causes Lassa fever (LF). LF is typically mild but it can cause severe disease characterized by hemorrhagic fever and multi-organ failure. A current outbreak of LASV in Nigeria has seen greater than 300 cases with a case fatality rate of 22%. Read More

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http://dx.doi.org/10.2147/DDDT.S147276DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6097522PMC
December 2018

Production of recombinant NS1 protein and its possible use in encephalitic flavivirus differential diagnosis.

Protein Expr Purif 2019 Jan 17;153:18-25. Epub 2018 Aug 17.

Área de Virosis Emergentes y Zoonóticas (AVEZ), Laboratorio de Ingeniería Genética y Biología Celular y Molecular (LIGBCM), Departamento de Ciencia y Tecnología (DCyT), Universidad Nacional de Quilmes (UNQ), Roque Sáenz Peña 352, B1876BXD, Bernal, Buenos Aires, Argentina. Electronic address:

Saint Louis encephalitis virus (SLEV) and West Nile virus (WNV) are two of the major causes of arboviral encephalitis in the Americas. The co-circulation of related flaviviruses in the Americas and prior vaccination against flaviviruses pose problems to the diagnostic specificity of serological assays due to the development of cross-reactive antibodies. An accurate diagnosis method capable of differentiating these related viruses is needed. Read More

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http://dx.doi.org/10.1016/j.pep.2018.08.008DOI Listing
January 2019
2 Reads

Review of Biology and Replication.

Front Microbiol 2018 3;9:1751. Epub 2018 Aug 3.

Department of Pathology, University of Texas Medical Branch at Galveston, Galveston, TX, United States.

The family is divided into three genera: , , and . The contain viruses responsible for causing human hemorrhagic fever diseases including New World viruses Junin, Machupo, Guanarito, Sabia, and Chapare virus and Old World viruses Lassa, and Lujo virus. These two groups of arenaviruses share the same genome organization composed of two ambisense RNA segments. Read More

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https://www.frontiersin.org/article/10.3389/fmicb.2018.01751
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http://dx.doi.org/10.3389/fmicb.2018.01751DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6085440PMC
August 2018
9 Reads
3.941 Impact Factor

Cholestasis induced liver pathology results in dysfunctional immune responses after arenavirus infection.

Sci Rep 2018 Aug 15;8(1):12179. Epub 2018 Aug 15.

Department of Molecular Medicine II, Medical Faculty, Heinrich Heine University, Universitätsstrasse. 1, 40225, Düsseldorf, Germany.

Immune responses are critical for defense against pathogens. However, prolonged viral infection can result in defective T cell immunity, leading to chronic viral infection. We studied immune activation in response to arenavirus infection during cholestasis using bile duct ligation (BDL). Read More

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http://dx.doi.org/10.1038/s41598-018-30627-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6093869PMC
August 2018
7 Reads

Visualization of Double-Stranded RNA Colocalizing With Pattern Recognition Receptors in Arenavirus Infected Cells.

Front Cell Infect Microbiol 2018 24;8:251. Epub 2018 Jul 24.

Department of Pathology and Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, TX, United States.

An important step in the initiation of the innate immune response to virus infection is the recognition of non-self, viral RNA, including double-stranded RNA (dsRNA), by cytoplasmic pattern recognition receptors (PRRs). For many positive-sense RNA viruses and DNA viruses, the production of viral dsRNA, and the interaction of viral dsRNA and PRRs are well characterized. However, for negative-sense RNA viruses, viral dsRNA was thought to be produced at low to undetectable levels and PRR recognition of viral dsRNA is still largely unclear. Read More

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http://dx.doi.org/10.3389/fcimb.2018.00251DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6066581PMC
July 2018
2 Reads

A genome-wide siRNA screen identifies a druggable host pathway essential for the Ebola virus life cycle.

Genome Med 2018 08 7;10(1):58. Epub 2018 Aug 7.

Laboratory of Virology, Division of Intramural Research, National Institute for Allergy and Infectious Diseases, National Institutes of Health, 903 S 4th St., Hamilton, MT, 59840, USA.

Background: The 2014-2016 Ebola virus (EBOV) outbreak in West Africa highlighted the need for improved therapeutic options against this virus. Approaches targeting host factors/pathways essential for the virus are advantageous because they can potentially target a wide range of viruses, including newly emerging ones and because the development of resistance is less likely than when targeting the virus directly. However, systematic approaches for screening host factors important for EBOV have been hampered by the necessity to work with this virus at biosafety level 4 (BSL4). Read More

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http://dx.doi.org/10.1186/s13073-018-0570-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6090742PMC
August 2018
23 Reads

Neutralization of Junín virus by single domain antibodies targeted against the nucleoprotein.

Sci Rep 2018 Jul 30;8(1):11451. Epub 2018 Jul 30.

VIB Center for Medical Biotechnology, Ghent, B-9052, Belgium.

The syndrome viral haemorrhagic fever (VHF) designates a broad range of diseases that are caused by different viruses including members of the family Arenaviridae. Prophylaxis for Argentine Haemorrhagic Fever (AHF), caused by the arenavirus Junín (JUNV), has been achieved by the use of a live attenuated vaccine, named Candid#1. The standard treatment of AHF is transfusion of convalescent human plasma. Read More

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http://dx.doi.org/10.1038/s41598-018-29508-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6065417PMC
July 2018
4 Reads

Lymphocytic choriomeningitis virus Clone 13 infection causes either persistence or acute death dependent on IFN-1, cytotoxic T lymphocytes (CTLs), and host genetics.

Proc Natl Acad Sci U S A 2018 08 30;115(33):E7814-E7823. Epub 2018 Jul 30.

Department of Immunology and Microbiology, The Scripps Research Institute, La Jolla, CA 92037.

Understanding of T cell exhaustion and successful therapy to restore T cell function was first described using Clone (Cl) 13 variant selected from the lymphocytic choriomeningitis virus (LCMV) Armstrong (ARM) 53b parental strain. T cell exhaustion plays a pivotal role in both persistent infections and cancers of mice and humans. C57BL/6, BALB, SWR/J, A/J, 129, C3H, and all but one collaborative cross (CC) mouse strain following Cl 13 infection have immunosuppressed T cell responses, high PD-1, and viral titers leading to persistent infection and normal life spans. Read More

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http://dx.doi.org/10.1073/pnas.1804674115DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6099862PMC
August 2018
23 Reads

Generation and Optimization of a Green Fluorescent Protein-Expressing Transcription and Replication-Competent Virus-Like Particle System for Ebola Virus.

J Infect Dis 2018 Nov;218(suppl_5):S360-S364

Institute for Molecular Virology and Cell Biology.

Work with infectious Ebola virus is restricted to biosafety level (BSL) 4 laboratories. To overcome this limitation, life cycle modeling systems, which recapitulate part or all of the virus life cycle under BSL-1 or -2 conditions, have been developed. The tetracistronic transcription and replication-competent virus-like particle (trVLP) system is currently the most advanced of these systems and is particularly useful for drug screening. Read More

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http://dx.doi.org/10.1093/infdis/jiy405DOI Listing
November 2018
4 Reads

The S Genome Segment Is Sufficient to Maintain Pathogenicity in Intra-Clade Lassa Virus Reassortants in a Guinea Pig Model.

Front Cell Infect Microbiol 2018 11;8:240. Epub 2018 Jul 11.

Viral Special Pathogens Branch, Division of High-Consequence Pathogens and Pathology, Centers for Disease Control and Prevention, Atlanta, GA, United States.

Genome reassortment in Lassa virus (LASV) has been reported in nature, but phenotypic consequences of this phenomenon are not well described. Here we characterize, both and , reassortment between 2 LASV strains: the prototypic 1976 Josiah strain and a more recently isolated 2015 Liberian strain. analysis showed that although cis- and trans-acting elements of viral RNA synthesis were compatible between strains, reassortants demonstrated different levels of viral replication. Read More

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http://dx.doi.org/10.3389/fcimb.2018.00240DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6050391PMC
July 2018
4 Reads

Lifecycle modelling systems support inosine monophosphate dehydrogenase (IMPDH) as a pro-viral factor and antiviral target for New World arenaviruses.

Antiviral Res 2018 Sep 19;157:140-150. Epub 2018 Jul 19.

Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Hamilton, MT, USA; Junior Research Group Arenavirus Biology, Friedrich-Loeffler-Institut, Greifswald, Insel Riems, Germany. Electronic address:

Infection with Junín virus (JUNV) is currently being effectively managed in the endemic region using a combination of targeted vaccination and plasma therapy. However, the long-term sustainability of plasma therapy is unclear and similar resources are not available for other New World arenaviruses. As a result, there has been renewed interest regarding the potential of drug-based therapies. Read More

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http://dx.doi.org/10.1016/j.antiviral.2018.07.009DOI Listing
September 2018
4 Reads

BST-2 controls T cell proliferation and exhaustion by shaping the early distribution of a persistent viral infection.

PLoS Pathog 2018 07 20;14(7):e1007172. Epub 2018 Jul 20.

Viral Immunology & Intravital Imaging Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland, United States of America.

The interferon inducible protein, BST-2 (or, tetherin), plays an important role in the innate antiviral defense system by inhibiting the release of many enveloped viruses. Consequently, viruses have evolved strategies to counteract the anti-viral activity of this protein. While the mechanisms by which BST-2 prevents viral dissemination have been defined, less is known about how this protein shapes the early viral distribution and immunological defense against pathogens during the establishment of persistence. Read More

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http://dx.doi.org/10.1371/journal.ppat.1007172DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6080785PMC
July 2018
2 Reads

Widespread arenavirus occurrence and seroprevalence in small mammals, Nigeria.

Parasit Vectors 2018 Jul 13;11(1):416. Epub 2018 Jul 13.

Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.

Background: Lassa fever, killing thousands of people annually, is the most reported viral zoonotic disease in Nigeria. Recently, different rodent species carrying diverse lineages of the Lassa virus (LASV) in addition to a novel Mobala-like genetic sequence were detected within the country. Here, screening 906 small mammal specimens from 11 localities for IgG antibodies and incorporating previous PCR detection data involving the same populations, we further describe arenavirus prevalence across Nigeria in relation to host species and geographical location. Read More

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http://dx.doi.org/10.1186/s13071-018-2991-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6045851PMC
July 2018
12 Reads

DDX3 suppresses type I interferons and favors viral replication during Arenavirus infection.

PLoS Pathog 2018 07 12;14(7):e1007125. Epub 2018 Jul 12.

Division of Biological Sciences, University of California San Diego, La Jolla, CA, United States of America.

Several arenaviruses cause hemorrhagic fever (HF) diseases that are associated with high morbidity and mortality in humans. Accordingly, HF arenaviruses have been listed as top-priority emerging diseases for which countermeasures are urgently needed. Because arenavirus nucleoprotein (NP) plays critical roles in both virus multiplication and immune-evasion, we used an unbiased proteomic approach to identify NP-interacting proteins in human cells. Read More

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http://dx.doi.org/10.1371/journal.ppat.1007125DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6042795PMC
July 2018
9 Reads

Isolation and genomic characterization of lymphocytic choriomeningitis virus in ticks from northeastern China.

Transbound Emerg Dis 2018 Dec 10;65(6):1733-1739. Epub 2018 Jul 10.

School of Life Sciences and Engineering, Foshan University, Foshan, China.

Lymphocytic choriomeningitis virus (LCMV) is a rodent-borne arenavirus that is considered a neglected cause of neurologic diseases in humans. In this study, we described genomic characterization of newly isolated LCMVs in Haemaphysalis longicornis, Dermacentor nuttalli, Dermacentor silvarum and Ixodes persulcatus in Jilin Province, northeastern China. The complete sequences of the small (S) and large (L) segments of LCMVs in ticks contained 3,375 and 7,235-7,241 nucleotides, respectively. Read More

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http://dx.doi.org/10.1111/tbed.12946DOI Listing
December 2018
11 Reads

Serological Evidence for the Circulation of Ebolaviruses in Pigs From Sierra Leone.

J Infect Dis 2018 Nov;218(suppl_5):S305-S311

Friedrich-Loeffler-Institut Institute of Novel and Emerging Infectious Diseases, Greifswald - Insel Riems, Germany.

Many human ebolavirus outbreaks have been linked to contact with wildlife including nonhuman primates and bats, which are assumed to serve as host species. However, it is largely unknown to what extent other animal species, particularly livestock, are involved in the transmission cycle or act as additional hosts for filoviruses. Pigs were identified as a susceptible host for Reston virus with subsequent transmission to humans reported in the Philippines. Read More

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http://dx.doi.org/10.1093/infdis/jiy330DOI Listing
November 2018
17 Reads

Transferrin and transferrin receptors update.

Authors:
Hiroshi Kawabata

Free Radic Biol Med 2019 Mar 30;133:46-54. Epub 2018 Jun 30.

Department of Hematology and Immunology, Kanazawa Medical University, 1-1 Daigaku, Uchinada-machi, Ishikawa-ken 920-0293, Japan. Electronic address:

In vertebrates, transferrin (Tf) safely delivers iron through circulation to cells. Tf-bound iron is incorporated through Tf receptor (TfR) 1-mediated endocytosis. TfR1 can mediate cellular uptake of both Tf and H-ferritin, an iron storage protein. Read More

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http://dx.doi.org/10.1016/j.freeradbiomed.2018.06.037DOI Listing
March 2019
24 Reads

CEACAM1 promotes CD8 T cell responses and improves control of a chronic viral infection.

Nat Commun 2018 07 2;9(1):2561. Epub 2018 Jul 2.

Institute of Immunology, University Hospital Essen, Hufelandstrasse 55, 45147, Essen, Germany.

Dysfunction of CD8 T cells can lead to the development of chronic viral infection. Identifying mechanisms responsible for such T cell dysfunction is therefore of great importance to understand how to prevent persistent viral infection. Here we show using lymphocytic choriomeningitis virus (LCMV) infection that carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) is fundamental for recruiting lymphocyte-specific protein kinase (Lck) into the T cell receptor complex to form an efficient immunological synapse. Read More

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http://dx.doi.org/10.1038/s41467-018-04832-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6028648PMC
July 2018
19 Reads
10.742 Impact Factor